BO94DRAFT_540805 cDNA ORF clone,

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gRNAs

The following BO94DRAFT_540805 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BO94DRAFT_540805 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs365266	XM_025612992.1 Latest version!	Aspergillus sclerotioniger CBS 115572 histidine-tagged glucoamylase (BO94DRAFT_540805), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BO94DRAFT_540805 ( XM_025612992.1 ) cDNA ORF clone, -> XP_025461462.1 Aspergillus sclerotioniger CBS 115572 histidine-tagged glucoamylase (BO94DRAFT_540805), mRNA.

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CloneID	OAs365266
Clone ID Related Accession (Same CDS sequence)	XM_025612992.1
Accession Version	XM_025612992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-11
Organism	Aspergillus sclerotioniger CBS 115572
Product	histidine-tagged glucoamylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_020291047). ##Metadata-START## Organism Display Name :: Aspergillus sclerotioniger CBS 115572 v1.0 GOLD Stamp ID :: Gp0047359 ##Metadata-END##

ATGTCGCTTC GATCTCTTCT CACCGTGAGC GGCCTCGTTT GTTCGGGCCT GGCCGGTGTT 
CTGCCCAAGC GGGCGACTCT AGATTCCTGG CTTAGCAGCG AAGCGACCGT GGCGCGTACC 
GCCATCCTGA ATAACATCGG GGCGGACGGC GCGTGGGTTT CGGGCGCGGA CTCGGGCATT 
GTCGTCGCCA GTCCCAGCAC GGATAACCCA GACTACTTTT ACACCTGGAC CCGTGACTCT 
GGCCTTGTCA TCAAGACCTT AGTCGATCTC TTCAGAAATG GAGACTCCGC CCTTCTCTCC 
ACCATCGAGC ACTACATTTC GTCGCAGGCA ATCGTCCAAG GCATTAGCAA CCCCTCTGGA 
GACCTCTCGT CGGGTGGATT GGGAGAACCC AAGTTCAATG TCGACGAGAC GGCCTTCACG 
GGGTCTTGGG GACGGCCTCA GCGGGATGGC CCGGCTTTGC GAGCCACCGC GATGATCGAC 
TTTGGCCAGT GGTTGATTGA AAATGGCTAC ACCAGTGTCG CGACCGATAT TGTTTGGCCC 
CTGGTTCGGA ACGATCTGTC GTATGTGGCG CAATACTGGA ACCAGACGGG ATATGATCTC 
TGGGAGGAAG TGAATGGTTC GTCTTTTTTT ACCATCGCTG TGCAGCACCG CGCCCTGGTG 
GAAGGTAGCA GTTTCGCGGC CAGCGTCGGC TCGTCCTGCT CGTGGTGCGA TTCACAGGCC 
CCTGAGATCC TCTGTTATCT GCAGTCTTTC TGGACGGGCG AATACATCTT GGCCAACTTT 
GATAGCAGTC GTTCTGGCAA GGATGCGAAC ACCCTCCTGG GAAGCATCCA CACCTTTGAC 
CCTGAGGCTG CATGTGATGA CACGACCTTC CAGCCGTGTT CGCCGCGTGC GCTTGCCAAC 
CACAAGGAGG TGGTCGACTC GTTCCGGTCG ATCTACACCA TCAACGATGG CTACACTACC 
AGCGAGGCCG TGGCGGTTGG TCGGTACCCC GAGGATTCGT ACTACAACGG TAACCCCTGG 
TTCTTGTGCA CACTGGCCGC GGCGGAGCAG CTGTACGACG CGCTCTACCA ATGGAACAAG 
CAGGGGTCTC TGGAGGTGAC CGACGTGTCG CTGGACTTTT TCCAGGCGCT GTACAGTGAT 
GCCGCAACGG GCACGTATTC CTCATCCAGC GCCACCTACA GCAGCATTGT GGATGCCGTC 
AAGACTTTTG CGGATGGCTT TGTTTCTATT GTCGAATCCC ATGCGGCCTC CAACGGCTCC 
TTGTCCGAGC AGTTCGGCAA GTCGGATGGC CTGGAACTCT CTGCCCGCGA CTTGACCTGG 
TCGTATGCTG CTCTGCTGAC TGCCAACAAC CGCCGTAACT CCGTTGTGCC GGCTTCCTGG 
GGTGAGACCT CTGCCAGCAG CGTGATCGGC ACCTGTGCGG CTACGTCTGC CTCTGGCACC 
TACAGCAGTG TGACTGTCAC CTCGTGGCCG ACGATCGTGG CTAGTGGTAC CACGTCTGCG 
ACGACTGCCA CGGGCACAGG CAGTGCGAAA TCTACCACGA GCAGCAAGAC CACCACCACT 
GCAAGCAAGA CGACGACCTC TTGCAGCACT CCTACTGCCG TGGCAGTGAC CTTTGATCTG 
ACCGAGACCA CCACCTACGG GGAGAATATC TACCTGAGCG GGTCGATCTC GCAGCTGGGT 
GACTGGGATA CCAGTGACGC CGTGGCGCTG AGTGCGGACA AGTACACCTC CAGCGACCCG 
TTGTGGTATG TGACGGTGAG CCTGCCCGCA GGCGAGTCGT TCGAGTACAA GTTCATCAAG 
GTTGAGTCGG ATGGCACAGT GGTATGGGAG AGCGACCCGA ACCGGGAATA CACTGTCCCT 
CAAGCGTGCG GCACATCGAC TGCGACGGTG ACTGATACAT GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025461462.1
CDS	170..2077
Translation	MSLRSLLTVSGLVCSGLAGVLPKRATLDSWLSSEATVARTAILNNIGADGAWVSGADSGIVVASPSTDNPDYFYTWTRDSGLVIKTLVDLFRNGDSALLSTIEHYISSQAIVQGISNPSGDLSSGGLGEPKFNVDETAFTGSWGRPQRDGPALRATAMIDFGQWLIENGYTSVATDIVWPLVRNDLSYVAQYWNQTGYDLWEEVNGSSFFTIAVQHRALVEGSSFAASVGSSCSWCDSQAPEILCYLQSFWTGEYILANFDSSRSGKDANTLLGSIHTFDPEAACDDTTFQPCSPRALANHKEVVDSFRSIYTINDGYTTSEAVAVGRYPEDSYYNGNPWFLCTLAAAEQLYDALYQWNKQGSLEVTDVSLDFFQALYSDAATGTYSSSSATYSSIVDAVKTFADGFVSIVESHAASNGSLSEQFGKSDGLELSARDLTWSYAALLTANNRRNSVVPASWGETSASSVIGTCAATSASGTYSSVTVTSWPTIVASGTTSATTATGTGSAKSTTSSKTTTTASKTTTSCSTPTAVAVTFDLTETTTYGENIYLSGSISQLGDWDTSDAVALSADKYTSSDPLWYVTVSLPAGESFEYKFIKVESDGTVVWESDPNREYTVPQACGTSTATVTDTWR

Target ORF information:

RefSeq Version	XM_025612992.1
Organism	Aspergillus sclerotioniger CBS 115572
Definition	Aspergillus sclerotioniger CBS 115572 histidine-tagged glucoamylase (BO94DRAFT_540805), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025612992.1

ORF Insert Sequence:

ATGTCGCTTC GATCTCTTCT CACCGTGAGC GGCCTCGTTT GTTCGGGCCT GGCCGGTGTT 
CTGCCCAAGC GGGCGACTCT AGATTCCTGG CTTAGCAGCG AAGCGACCGT GGCGCGTACC 
GCCATCCTGA ATAACATCGG GGCGGACGGC GCGTGGGTTT CGGGCGCGGA CTCGGGCATT 
GTCGTCGCCA GTCCCAGCAC GGATAACCCA GACTACTTTT ACACCTGGAC CCGTGACTCT 
GGCCTTGTCA TCAAGACCTT AGTCGATCTC TTCAGAAATG GAGACTCCGC CCTTCTCTCC 
ACCATCGAGC ACTACATTTC GTCGCAGGCA ATCGTCCAAG GCATTAGCAA CCCCTCTGGA 
GACCTCTCGT CGGGTGGATT GGGAGAACCC AAGTTCAATG TCGACGAGAC GGCCTTCACG 
GGGTCTTGGG GACGGCCTCA GCGGGATGGC CCGGCTTTGC GAGCCACCGC GATGATCGAC 
TTTGGCCAGT GGTTGATTGA AAATGGCTAC ACCAGTGTCG CGACCGATAT TGTTTGGCCC 
CTGGTTCGGA ACGATCTGTC GTATGTGGCG CAATACTGGA ACCAGACGGG ATATGATCTC 
TGGGAGGAAG TGAATGGTTC GTCTTTTTTT ACCATCGCTG TGCAGCACCG CGCCCTGGTG 
GAAGGTAGCA GTTTCGCGGC CAGCGTCGGC TCGTCCTGCT CGTGGTGCGA TTCACAGGCC 
CCTGAGATCC TCTGTTATCT GCAGTCTTTC TGGACGGGCG AATACATCTT GGCCAACTTT 
GATAGCAGTC GTTCTGGCAA GGATGCGAAC ACCCTCCTGG GAAGCATCCA CACCTTTGAC 
CCTGAGGCTG CATGTGATGA CACGACCTTC CAGCCGTGTT CGCCGCGTGC GCTTGCCAAC 
CACAAGGAGG TGGTCGACTC GTTCCGGTCG ATCTACACCA TCAACGATGG CTACACTACC 
AGCGAGGCCG TGGCGGTTGG TCGGTACCCC GAGGATTCGT ACTACAACGG TAACCCCTGG 
TTCTTGTGCA CACTGGCCGC GGCGGAGCAG CTGTACGACG CGCTCTACCA ATGGAACAAG 
CAGGGGTCTC TGGAGGTGAC CGACGTGTCG CTGGACTTTT TCCAGGCGCT GTACAGTGAT 
GCCGCAACGG GCACGTATTC CTCATCCAGC GCCACCTACA GCAGCATTGT GGATGCCGTC 
AAGACTTTTG CGGATGGCTT TGTTTCTATT GTCGAATCCC ATGCGGCCTC CAACGGCTCC 
TTGTCCGAGC AGTTCGGCAA GTCGGATGGC CTGGAACTCT CTGCCCGCGA CTTGACCTGG 
TCGTATGCTG CTCTGCTGAC TGCCAACAAC CGCCGTAACT CCGTTGTGCC GGCTTCCTGG 
GGTGAGACCT CTGCCAGCAG CGTGATCGGC ACCTGTGCGG CTACGTCTGC CTCTGGCACC 
TACAGCAGTG TGACTGTCAC CTCGTGGCCG ACGATCGTGG CTAGTGGTAC CACGTCTGCG 
ACGACTGCCA CGGGCACAGG CAGTGCGAAA TCTACCACGA GCAGCAAGAC CACCACCACT 
GCAAGCAAGA CGACGACCTC TTGCAGCACT CCTACTGCCG TGGCAGTGAC CTTTGATCTG 
ACCGAGACCA CCACCTACGG GGAGAATATC TACCTGAGCG GGTCGATCTC GCAGCTGGGT 
GACTGGGATA CCAGTGACGC CGTGGCGCTG AGTGCGGACA AGTACACCTC CAGCGACCCG 
TTGTGGTATG TGACGGTGAG CCTGCCCGCA GGCGAGTCGT TCGAGTACAA GTTCATCAAG 
GTTGAGTCGG ATGGCACAGT GGTATGGGAG AGCGACCCGA ACCGGGAATA CACTGTCCCT 
CAAGCGTGCG GCACATCGAC TGCGACGGTG ACTGATACAT GGCGGTGA