LOC114145015 cDNA ORF clone,

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gRNAs

The following LOC114145015 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC114145015 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMo186273	XM_028018333.1 Latest version!	Xiphophorus couchianus TGF-beta receptor type-2-like (LOC114145015), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC114145015 ( XM_028018333.1 ) cDNA ORF clone, -> XP_027874134.1 Xiphophorus couchianus TGF-beta receptor type-2-like (LOC114145015), mRNA.

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CloneID	OMo186273
Clone ID Related Accession (Same CDS sequence)	XM_028018333.1
Accession Version	XM_028018333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-10
Organism	Xiphophorus couchianus(Monterrey platyfish)
Product	TGF-beta receptor type-2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040232.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus couchianus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGGTT TCAGAAGCTG CCTCAGTAAC ATGCACGGAC CGCAGTGCTG CTCATCGTCA 
CGGGGAGTTT CGTTACCATC ATCATTAGTA TTATGGGCTG TTTTCTTTTT GCCGCTGCTC 
AAACCTGGGA CAGCTGGTAT ACCAGGCATG TCCATGTACC CCATCAGTCG GCTGTGTAAG 
TTCTGTGACC GACAGAGTTC CTCCTGCAAT GGCACAGAGA GCTGCATGTC CCACTGCTCC 
ATTACGTCTG TCTGTGATTC GCGTGAAGAG GTCTGTGTTG CTATATGGAT AAAGAATGAG 
ACCAGCTTCA CCGTGGAGAC CCTTTGCCAC AACCCGTCCG TGTCGCTGTA CGGCATCATG 
CTGGACGACT ACAACAGCTC CAGCTGTGTG ATGAAGGAGA AGAACTCCAC CAGCGGGATG 
GTGCACGTCT GTTCCTGCAC AGAAGAAGAG TGTAATGACA AAATGGTGTT CGTTCCCAAA 
GCAAAAGATC AAGCCGTGGG CGATTCCCTC ATCTCTATTA TTCTGGTAAG CCTGCTTCCC 
CTTATTGTGA TGGGAGTCTT GGTTGCCGCG GTGATCCACT GGTACTGCGT CAATCGGCGG 
CGCCATCTTA GCAAGAAGTG GGAGAGCAAC GTTAAAATGC GTAAGCCAAA AGCCGGCGGG 
TTGGACTGCA GAGACGCCTG CGCCTTCATG GTGGATGACG ACGGCTCTGA CAGCAGCTCC 
ATTCACGCCA ACAACCTGAA TCACAACACC GAACCTCTGC CTATAGAGCT GGATCTGCTG 
GTGGGTAAGG GCCGCTTCGC TCAGGTGTAC AAGGCAAAGC TGAAGCAGAG CAGCTCAGCT 
CAGTTTGAAA CCGTGGCTGT GAAGATCTTC CCCTCTGAGG AGTACGCATC CTGGAAGAAC 
GAGAAGGACA TCTTTTCCAA CAAGGACCTC AGACATGAGA ACGTCCTCCA CTTTCTGACA 
GCTGAGGAGA GGAAGGCAGA GAAACAGTAC TGGCTCATCA CAGCTTTCCA CCCCAGTGGG 
AACCTCCAGG AGTATCTGAC GCGCCACGTG ATCAGCTGGG AGGAACTGCA GGCGTTGAGC 
AGCTCTCTGG CCCGCGGAGT CGCCCACCTT CACAGCGACC GGCTCGCAAG TGGACGGCCC 
AAGGTGCCCA TAGTTCACAG AGACCTAAAG AGCTCCAACG TGCTGGTGAA GAACGACCTG 
ACCTGCTGCA TATGTGACTT TGGCCTGGCG CTCTGTCTGG ACAGCAGCCT GTCAGTGGAT 
GATCTCGCCA ACAGCGGCCA GGTGGGTACG GCGCGCTACA TGGCCCCTGA GGTCCTGGAG 
GCCCGGCTCG ATCTGGAGAA CATCGAGTCC TTCAAGCAGA CCGACATTTA CTCCATGGCT 
CTGGTCCTGT GGGAAATGAC ATCAAGATGC AAAACCATAG GAGAGGTGAA GGACTATGAG 
CCAGCGTTCG GCTCCAAGGT GCGACAGCAC CCCTATGTGG AGAGCATGAA GGACAACGAC 
AGACCCGACA TCCCCGACAG CTGGCTCCGG CATCAGGGCA TTTTGGCTAT CTGCACCACT 
ATCAAGGAAT GCTGGGACCA CGACCCGGAG GCCCGTCTCA CGGCCCACTG CGTGGCCGAG 
CGACTCTCTG AGATCGAGGA GGAGCTGGAC AAGCTGTCGA GCCGCAGCTC CTCGGCTGAG 
AAGATCCGAT ACGAGGTGCA CATCCCCGTC GCCATGGAGA TCCCAGAGGA GGAGGTGAAG 
ATCTCCGAAA TCCAGAACAT CATAGCTGCG GACTGCTCTG TGAGCGACAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027874134.1
CDS	134..1930
Translation	MNGFRSCLSNMHGPQCCSSSRGVSLPSSLVLWAVFFLPLLKPGTAGIPGMSMYPISRLCKFCDRQSSSCNGTESCMSHCSITSVCDSREEVCVAIWIKNETSFTVETLCHNPSVSLYGIMLDDYNSSSCVMKEKNSTSGMVHVCSCTEEECNDKMVFVPKAKDQAVGDSLISIILVSLLPLIVMGVLVAAVIHWYCVNRRRHLSKKWESNVKMRKPKAGGLDCRDACAFMVDDDGSDSSSIHANNLNHNTEPLPIELDLLVGKGRFAQVYKAKLKQSSSAQFETVAVKIFPSEEYASWKNEKDIFSNKDLRHENVLHFLTAEERKAEKQYWLITAFHPSGNLQEYLTRHVISWEELQALSSSLARGVAHLHSDRLASGRPKVPIVHRDLKSSNVLVKNDLTCCICDFGLALCLDSSLSVDDLANSGQVGTARYMAPEVLEARLDLENIESFKQTDIYSMALVLWEMTSRCKTIGEVKDYEPAFGSKVRQHPYVESMKDNDRPDIPDSWLRHQGILAICTTIKECWDHDPEARLTAHCVAERLSEIEEELDKLSSRSSSAEKIRYEVHIPVAMEIPEEEVKISEIQNIIAADCSVSDKK

Target ORF information:

RefSeq Version	XM_028018333.1
Organism	Xiphophorus couchianus(Monterrey platyfish)
Definition	Xiphophorus couchianus TGF-beta receptor type-2-like (LOC114145015), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028018333.1

ORF Insert Sequence:

ATGAACGGTT TCAGAAGCTG CCTCAGTAAC ATGCACGGAC CGCAGTGCTG CTCATCGTCA 
CGGGGAGTTT CGTTACCATC ATCATTAGTA TTATGGGCTG TTTTCTTTTT GCCGCTGCTC 
AAACCTGGGA CAGCTGGTAT ACCAGGCATG TCCATGTACC CCATCAGTCG GCTGTGTAAG 
TTCTGTGACC GACAGAGTTC CTCCTGCAAT GGCACAGAGA GCTGCATGTC CCACTGCTCC 
ATTACGTCTG TCTGTGATTC GCGTGAAGAG GTCTGTGTTG CTATATGGAT AAAGAATGAG 
ACCAGCTTCA CCGTGGAGAC CCTTTGCCAC AACCCGTCCG TGTCGCTGTA CGGCATCATG 
CTGGACGACT ACAACAGCTC CAGCTGTGTG ATGAAGGAGA AGAACTCCAC CAGCGGGATG 
GTGCACGTCT GTTCCTGCAC AGAAGAAGAG TGTAATGACA AAATGGTGTT CGTTCCCAAA 
GCAAAAGATC AAGCCGTGGG CGATTCCCTC ATCTCTATTA TTCTGGTAAG CCTGCTTCCC 
CTTATTGTGA TGGGAGTCTT GGTTGCCGCG GTGATCCACT GGTACTGCGT CAATCGGCGG 
CGCCATCTTA GCAAGAAGTG GGAGAGCAAC GTTAAAATGC GTAAGCCAAA AGCCGGCGGG 
TTGGACTGCA GAGACGCCTG CGCCTTCATG GTGGATGACG ACGGCTCTGA CAGCAGCTCC 
ATTCACGCCA ACAACCTGAA TCACAACACC GAACCTCTGC CTATAGAGCT GGATCTGCTG 
GTGGGTAAGG GCCGCTTCGC TCAGGTGTAC AAGGCAAAGC TGAAGCAGAG CAGCTCAGCT 
CAGTTTGAAA CCGTGGCTGT GAAGATCTTC CCCTCTGAGG AGTACGCATC CTGGAAGAAC 
GAGAAGGACA TCTTTTCCAA CAAGGACCTC AGACATGAGA ACGTCCTCCA CTTTCTGACA 
GCTGAGGAGA GGAAGGCAGA GAAACAGTAC TGGCTCATCA CAGCTTTCCA CCCCAGTGGG 
AACCTCCAGG AGTATCTGAC GCGCCACGTG ATCAGCTGGG AGGAACTGCA GGCGTTGAGC 
AGCTCTCTGG CCCGCGGAGT CGCCCACCTT CACAGCGACC GGCTCGCAAG TGGACGGCCC 
AAGGTGCCCA TAGTTCACAG AGACCTAAAG AGCTCCAACG TGCTGGTGAA GAACGACCTG 
ACCTGCTGCA TATGTGACTT TGGCCTGGCG CTCTGTCTGG ACAGCAGCCT GTCAGTGGAT 
GATCTCGCCA ACAGCGGCCA GGTGGGTACG GCGCGCTACA TGGCCCCTGA GGTCCTGGAG 
GCCCGGCTCG ATCTGGAGAA CATCGAGTCC TTCAAGCAGA CCGACATTTA CTCCATGGCT 
CTGGTCCTGT GGGAAATGAC ATCAAGATGC AAAACCATAG GAGAGGTGAA GGACTATGAG 
CCAGCGTTCG GCTCCAAGGT GCGACAGCAC CCCTATGTGG AGAGCATGAA GGACAACGAC 
AGACCCGACA TCCCCGACAG CTGGCTCCGG CATCAGGGCA TTTTGGCTAT CTGCACCACT 
ATCAAGGAAT GCTGGGACCA CGACCCGGAG GCCCGTCTCA CGGCCCACTG CGTGGCCGAG 
CGACTCTCTG AGATCGAGGA GGAGCTGGAC AAGCTGTCGA GCCGCAGCTC CTCGGCTGAG 
AAGATCCGAT ACGAGGTGCA CATCCCCGTC GCCATGGAGA TCCCAGAGGA GGAGGTGAAG 
ATCTCCGAAA TCCAGAACAT CATAGCTGCG GACTGCTCTG TGAGCGACAA GAAGTGA