twsg1 cDNA ORF clone,

Gene
Clones
gRNAs

The following twsg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twsg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIn01548	XM_024281037.1 Latest version!	Oryzias melastigma twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

twsg1 ( XM_024281037.1 ) cDNA ORF clone, -> XP_024136805.1 Oryzias melastigma twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.

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CloneID	OIn01548
Clone ID Related Accession (Same CDS sequence)	XM_024281037.1
Accession Version	XM_024281037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-10
Organism	Oryzias melastigma(Indian medaka)
Product	twisted gastrulation protein homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020096074.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias melastigma Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCTG CTCAGCTGAT CCTCCCCGCC ACCGCCGCTC TCCTCTTCGT CCTGTCAGGA 
CTGTCTCTGA CCTCTGGCTG TAACAAAGCC CTGTGTGCCA GCGACGTCAG CAAATGTCTC 
ATCCAGGAGC TTTGCCAGTG CCGTCCCTCC GATGAGAACT GCTCCTGCTG CAAAGACTGC 
ATGCTTTGTC TGGGGAACCT CTGGGAGGAG TGCTGCGACT GCGTGGGGAT GTGTAACCCC 
AAGAACTACA ACGACTCTCC GGCCACATCC AAGAGCACGG TGGAGGAGCT GCACCGCCCC 
ATCCCCTCGC TGTTCCGTGC TCTGACGGAG GGTGACGCTC CCATAAACAT GATGGTGGTG 
TCGTTCCCCG TCGCGGAGGA GCTCTCGCAT CACGAGAACT TGGTATCGTT TCTGGAGTCG 
CTCGACAGCC AGGATCCCAA CGTGTCAAAG GCCAGGAACA GCCTGCACCC TGGCTACGAC 
AATCAAGAAA ATATGTGCAC AGTGGTGTAC TTTGACGACT GCGTGTCCAT CCACCAGTGC 
AAACTGTATT GCGAGTCGAT GGGAGGGTCG AAGTACCGGT GGTTTCACAA CGCCTGCTGC 
CAGTGCATCG GGCCGGAGTG CGTGGACTAC GGCAGCAAGG CCGTGAAGTG CTTGAACTGT 
CTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024136805.1
CDS	607..1275
Translation	MRPAQLILPATAALLFVLSGLSLTSGCNKALCASDVSKCLIQELCQCRPSDENCSCCKDCMLCLGNLWEECCDCVGMCNPKNYNDSPATSKSTVEELHRPIPSLFRALTEGDAPINMMVVSFPVAEELSHHENLVSFLESLDSQDPNVSKARNSLHPGYDNQENMCTVVYFDDCVSIHQCKLYCESMGGSKYRWFHNACCQCIGPECVDYGSKAVKCLNCLF

Target ORF information:

RefSeq Version	XM_024281037.1
Organism	Oryzias melastigma(Indian medaka)
Definition	Oryzias melastigma twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024281037.1

ORF Insert Sequence:

ATGAGGCCTG CTCAGCTGAT CCTCCCCGCC ACCGCCGCTC TCCTCTTCGT CCTGTCAGGA 
CTGTCTCTGA CCTCTGGCTG TAACAAAGCC CTGTGTGCCA GCGACGTCAG CAAATGTCTC 
ATCCAGGAGC TTTGCCAGTG CCGTCCCTCC GATGAGAACT GCTCCTGCTG CAAAGACTGC 
ATGCTTTGTC TGGGGAACCT CTGGGAGGAG TGCTGCGACT GCGTGGGGAT GTGTAACCCC 
AAGAACTACA ACGACTCTCC GGCCACATCC AAGAGCACGG TGGAGGAGCT GCACCGCCCC 
ATCCCCTCGC TGTTCCGTGC TCTGACGGAG GGTGACGCTC CCATAAACAT GATGGTGGTG 
TCGTTCCCCG TCGCGGAGGA GCTCTCGCAT CACGAGAACT TGGTATCGTT TCTGGAGTCG 
CTCGACAGCC AGGATCCCAA CGTGTCAAAG GCCAGGAACA GCCTGCACCC TGGCTACGAC 
AATCAAGAAA ATATGTGCAC AGTGGTGTAC TTTGACGACT GCGTGTCCAT CCACCAGTGC 
AAACTGTATT GCGAGTCGAT GGGAGGGTCG AAGTACCGGT GGTTTCACAA CGCCTGCTGC 
CAGTGCATCG GGCCGGAGTG CGTGGACTAC GGCAGCAAGG CCGTGAAGTG CTTGAACTGT 
CTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.