bmp4 cDNA ORF clone,

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gRNAs

The following bmp4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmp4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIn01285	XM_024277561.1 Latest version!	Oryzias melastigma bone morphogenetic protein 4 (bmp4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bmp4 ( XM_024277561.1 ) cDNA ORF clone, -> XP_024133329.1 Oryzias melastigma bone morphogenetic protein 4 (bmp4), mRNA.

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CloneID	OIn01285
Clone ID Related Accession (Same CDS sequence)	XM_024277561.1
Accession Version	XM_024277561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-10
Organism	Oryzias melastigma(Indian medaka)
Product	bone morphogenetic protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020096073.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias melastigma Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCT GCAGCCTCCC AGCACCACCT AACATTTCCT GGAAAAACGT GTCCATTGCC 
TGCTCCCAAC AAACATGGAC TGTTTTCCCA TGCTCTATTT TCTGTCGAGA CATCATGATT 
CCTGGTAATC GAATGCTGAT GGTCATTTTA ATATCCCAAG TCCTGCTGGG AGAGAGCAAC 
CATGCTAGTC TGATACCCGA AGAAGGGAAA AAGAAGGTAC CTGGCCTGCA GGGTCGCTCG 
ACCGCTCAGA GCCATGAACT TTTACGGGAC TTCGAGGCCA CGCTGTTGCA CATGTTTGGC 
CTCAAAAAGC GCCCGCGTCC AAGCAAGTCC GCCGCCGTGC CCCGCTACCT GCTGGATCTC 
TATCGACTGC AGTCCGGGGA GGCTGAGGAC GTAGAGGCAC ACGACATTCC TTTTGAGTAT 
CCCGAGAAGA CAGCCAGCCG GGCCAACACC GTCAGGGGCT TCCACCATGA AGAGCACATG 
GAGAGGATAC ATGAAGATGA TGGAGAATCC ATTCCTCTTC GCTTCCTGTT CAACCTGAGC 
AGCATTCCAG AGGACGAGCT GCTGTCTTCC GCAGAGCTGC GACTCTACCG TCAGCAGCTG 
GATGAGGCCA GCGCTGATAG CCTCCTAGAC GAGCCGGGCC TTCATCGGAT AAACATATAC 
GAGGTGCTTA AGCCCCCGCG GCCCGGGCAG CTTATCACTC AGCTCCTGGA CACCCGGCTC 
GTGCACCACA ACGCTTCGCG CTGGGAGAGC TTTGACGTCA GCCCCGCTGT GCTGCGTTGG 
ACTCGGGAAC GGCTCCCTAA TTATGGACTG GCTGTGGAGG TTCTGCACCT TAATCAGACT 
CCACACAACC AGCACCGACA CGTCCGCATC AGCCGTTCCT TACATCAGGA GCCAGGAGAG 
GACTGGGACC AGGTACGCCC TCTTCTGGTT ACCTTTGGCC ACGATGGAAA GGGTCACTCA 
TTGACTCGCC GGACGAAACG CAGCCCCAAG CAACGGGGGC GTAAGCGGAA CCGCAACTGC 
CGGCGCCACG CCCTGTACGT GGACTTTAGC GATGTAGGCT GGAACGACTG GATAGTGGCG 
CCTCCCGGCT ACGAGGCCTA TTACTGCCAC GGAGAGTGCC CTTTTCCTCT GGCAGATCAT 
CTGAACTCAA CGAACCACGC CATCGTTCAG ACACTGGTGA ACTCTGTGAA CAACAAAATT 
CCCAAGGCCT GCTGCATACC AACAGAGCTC AGCGCCATCT CAATGCTCTA CCTAGACGAA 
TACGACAAGG TGGTCCTAAA AAACTACCAG GAAATGGTGG TGGAGGGCTG CGGCTGCCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024133329.1
CDS	399..1721
Translation	MFSCSLPAPPNISWKNVSIACSQQTWTVFPCSIFCRDIMIPGNRMLMVILISQVLLGESNHASLIPEEGKKKVPGLQGRSTAQSHELLRDFEATLLHMFGLKKRPRPSKSAAVPRYLLDLYRLQSGEAEDVEAHDIPFEYPEKTASRANTVRGFHHEEHMERIHEDDGESIPLRFLFNLSSIPEDELLSSAELRLYRQQLDEASADSLLDEPGLHRINIYEVLKPPRPGQLITQLLDTRLVHHNASRWESFDVSPAVLRWTRERLPNYGLAVEVLHLNQTPHNQHRHVRISRSLHQEPGEDWDQVRPLLVTFGHDGKGHSLTRRTKRSPKQRGRKRNRNCRRHALYVDFSDVGWNDWIVAPPGYEAYYCHGECPFPLADHLNSTNHAIVQTLVNSVNNKIPKACCIPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR

Target ORF information:

RefSeq Version	XM_024277561.1
Organism	Oryzias melastigma(Indian medaka)
Definition	Oryzias melastigma bone morphogenetic protein 4 (bmp4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024277561.1

ORF Insert Sequence:

ATGTTCTCCT GCAGCCTCCC AGCACCACCT AACATTTCCT GGAAAAACGT GTCCATTGCC 
TGCTCCCAAC AAACATGGAC TGTTTTCCCA TGCTCTATTT TCTGTCGAGA CATCATGATT 
CCTGGTAATC GAATGCTGAT GGTCATTTTA ATATCCCAAG TCCTGCTGGG AGAGAGCAAC 
CATGCTAGTC TGATACCCGA AGAAGGGAAA AAGAAGGTAC CTGGCCTGCA GGGTCGCTCG 
ACCGCTCAGA GCCATGAACT TTTACGGGAC TTCGAGGCCA CGCTGTTGCA CATGTTTGGC 
CTCAAAAAGC GCCCGCGTCC AAGCAAGTCC GCCGCCGTGC CCCGCTACCT GCTGGATCTC 
TATCGACTGC AGTCCGGGGA GGCTGAGGAC GTAGAGGCAC ACGACATTCC TTTTGAGTAT 
CCCGAGAAGA CAGCCAGCCG GGCCAACACC GTCAGGGGCT TCCACCATGA AGAGCACATG 
GAGAGGATAC ATGAAGATGA TGGAGAATCC ATTCCTCTTC GCTTCCTGTT CAACCTGAGC 
AGCATTCCAG AGGACGAGCT GCTGTCTTCC GCAGAGCTGC GACTCTACCG TCAGCAGCTG 
GATGAGGCCA GCGCTGATAG CCTCCTAGAC GAGCCGGGCC TTCATCGGAT AAACATATAC 
GAGGTGCTTA AGCCCCCGCG GCCCGGGCAG CTTATCACTC AGCTCCTGGA CACCCGGCTC 
GTGCACCACA ACGCTTCGCG CTGGGAGAGC TTTGACGTCA GCCCCGCTGT GCTGCGTTGG 
ACTCGGGAAC GGCTCCCTAA TTATGGACTG GCTGTGGAGG TTCTGCACCT TAATCAGACT 
CCACACAACC AGCACCGACA CGTCCGCATC AGCCGTTCCT TACATCAGGA GCCAGGAGAG 
GACTGGGACC AGGTACGCCC TCTTCTGGTT ACCTTTGGCC ACGATGGAAA GGGTCACTCA 
TTGACTCGCC GGACGAAACG CAGCCCCAAG CAACGGGGGC GTAAGCGGAA CCGCAACTGC 
CGGCGCCACG CCCTGTACGT GGACTTTAGC GATGTAGGCT GGAACGACTG GATAGTGGCG 
CCTCCCGGCT ACGAGGCCTA TTACTGCCAC GGAGAGTGCC CTTTTCCTCT GGCAGATCAT 
CTGAACTCAA CGAACCACGC CATCGTTCAG ACACTGGTGA ACTCTGTGAA CAACAAAATT 
CCCAAGGCCT GCTGCATACC AACAGAGCTC AGCGCCATCT CAATGCTCTA CCTAGACGAA 
TACGACAAGG TGGTCCTAAA AAACTACCAG GAAATGGTGG TGGAGGGCTG CGGCTGCCGC 
TGA