bmp2k cDNA ORF clone,

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Clones
gRNAs

The following bmp2k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmp2k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIn32266	XM_024260277.1 Latest version!	Oryzias melastigma BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OIn32267	XM_024260278.1 Latest version!	Oryzias melastigma BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bmp2k ( XM_024260277.1 ) cDNA ORF clone, -> XP_024116045.1 Oryzias melastigma BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIn32266
Clone ID Related Accession (Same CDS sequence)	XM_024260277.1
Accession Version	XM_024260277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-10
Organism	Oryzias melastigma(Indian medaka)
Product	BMP-2-inducible protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020096109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias melastigma Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTCCAGGAT GCCAAAGTCC GAAAGCGGCG GGCTCGGAGG GGTGTCCGGC 
TCCGGGCTGA GCAGCTACTT CGGGAAAGTG TTCGCGGTGG GCCGGTACCA GGTCACGGTG 
GAGGAGCTCG TCGCAGAGGG AGGCTTCTCT GTGGTGTTTA TGGCTCGAAC ACACAGTGGT 
GTCCGCTGCG CTCTCAAGAG GATGTACGTG AACAATGTGC AAGACCTGAA CATTTGCAAG 
AGAGAGATCA CTATCATGAA AGAGCTGTCA GGTCACAAGA ACATCGTCAC CTACCTGGAC 
TCCACCATCA GTTCTTTTTC AGACAATGTT TGGGAGGTCC TTATCCTCAT GGAGTTCTGC 
AAAGCGGGTC AGGTGGTGAA ACAGATGAAC CAGAGGTTAA ATGTGGGCTT CAGTGAAGCT 
GAAGTCCTGC AGATTTTCTG CGACACCTGC GAGGCCGTGG CTCGCCTGCA CCAATGCAAG 
ACCCCCATCA TCCACAGAGA CCTGAAAGTG GAGAACATTC TGCTGAATGA CCAGGGGAAC 
TATGTGCTGT GTGACTTTGG CAGTGCCACT CACAAAGTCT TACTGCCTCA CAAGGATGGA 
GTGACTGCTG TGGAGGATGA AATCAAAAAG TACACGACTC TTTCATATCG GGCTCCAGAG 
ATGATTAACT TGTATGCAGG AAAAGGCATC ACCACTAAGG CTGATATTTG GGCTCTGGGA 
TGCTTGCTGT ACAAGCTGTG TTTCTTCACT CTTCCTTTTG GAGAGAGTCA AGTTGCCATT 
TGTGACGGGA GCTTCATTGT TCCAGATAAC TCCAAGTTCT CTTGCAAACT GCACTGCTTG 
ATCAGATATA TGCTGGAGCC AGACCTGGAG AAGAGACCAG ACATTTACCA AGTGTCCCAC 
TTTGCCTTTA AGATAGCTGG AAGAGATAAT CCTGTACCAA ACCTCTGTAG CTCTCCCATC 
CCTACATCAC TCCCTGAGCC GCTCACAGCC AGCGAGGTTG CTGCCAAAAA GAATATGACA 
AAAGCCAGGA TTACAGACTC TGTTGTTCCA ACTGAGACCT CTATAGCTCC CAGGCAGAGA 
CCAAAGGCTG CAAACTCCAA CGTTCTCCCC CTCACTAACA CTGTCACCCC CGTCAAGATG 
ACCGTGCCTT CCACAGCCAT CAGCAATGGC CAGAAAGTCC CTACTCCCAG CTCTGGACAG 
CCATCCATAC AGGCCCAGCC CAGCAGTCAG CAGAACCGAG TCCTGCAGCA GCTGCAGCCA 
GGTGATCTGC GTTTGCAGCA GCTGCAGCAA CAACAAGGAG TGCAGCAGCA GCATGCAAAT 
GTGCAGCAAC TCCAGTACCT TCAGTATCAA CAGGCAGTGC AGCAGTCCCT CCAGCAGCAG 
CAGCAAGTCT TGCTCCAGCA GCAGCAGATG ATGATGCATC CCATGTACCA GCAGCAGGTT 
GCACAGGCCC AGTATGCTGC TATGCTGCAC CAGTACCAAC AGTCCTTTGT CCAGCAGCAG 
CAGCAGCAGT ATCATCCCGC TCAGCAGCCT CTCCCCTACA TGTCTTCCCC TCTTGACTTC 
CAGATCCCTC TGGGGCCCTA CAATGCCACT ACTCCCACTG CTGGGGCTGC TCAGATGGGA 
GGACCATCAC CTCTGGATCC TTCATACTCC ACCCCCAGCA GCAGTAGAAA CCCCCTGGTG 
GTCTCAGATG GAATCGCCCC TCCTTCTCAG ACCGGCGGCG CCATCAGTAA CCCTCCCGAC 
ATGTCGAGCT GGAACCCGTT TGGAGAGGAC AACTTCTCCA AACTAACGGA GGAAGAGCTG 
CTGGACCGGG AGTTTGACCT GCTCAGAGCA AAAAAGCCGG CAGAGAGGAC AGCCAGCACG 
GACGCTGACC AACCGCCACC TCCCAACAAG CCCCCCCCAT CAGAGGACCT GTTTGGCTCC 
ATCCCTTTTG TGGCCAACAC AGGTGCTGCT GTGATGAAAG GTGAGGCACC TGCTGTGGAG 
GCTGGCGTCT GCCCCCCCAC CTCCACAGCC CTAGCTAAGG GGCCCAAACT GGGCAAAAAG 
AGCGGCTGCA GACCTGGAGA AGACTCAGAC AGCGACTTTG AGTCGGATCC TCCTTCTCCA 
AAGAGCAGTG AGGATGAGGA GCAGGAGGAA GAGGAGGCCC TGAACAGTGA GCACAGCCAA 
GACACTGAGC CAGAGAACTT GGGACAGAGA CCTTTGCTGA TGGACTCTGA GGAGGAGGGG 
GACAAGCGCA GCTCTGATTC AGAGTCTGAG CCTCCCAGGA CAAAGAACCG ATCCAGGAAG 
CCTGCAGGGA GCAAAGGAGG AGGAGCAGCT GCTGCCGGCA GGAGCACTCC AGAAGCCTCT 
GTGGCTGCTC CTGTCACCCC CCATCCTGCG TTGCTCAGAG GAACCAGAGC CTCAGAGGCT 
GTAGATATGT TTGGCGCCTT GCCCTTTGTG GGGGGCACCT CCCCTGTGGG GGCCTCACAG 
AGTTCTGCTG TGTTTGCACC TTTCGGGCAG GATGAGGAGC TGGCTGCAGA GGAGTTTGAT 
GTTTTCATCA AAGCTCCCTT CAGCAGGAAC CCCTCAAAGA CGGGAAGAAG CAGCAATGAA 
GCCCCCCTGT CCCCCGATTC CATTGACGTG TTTGGATTCT CTCCTTTCCA GCCGGGCCCT 
GTTGGACCAC CTCCAAGCAC TTCCAGGAGC GCCGAAAACA TCTTCCACCT CCAGGAGCCT 
GCCAGTCCCC GGCAGCAGAG GCTGAAGCAG CGCAGCCTGC AGAAGCTGTC CTTGCGCCAG 
AGGAAGACCA AGCAGGAGGC AGCCGGGGTA GGCGGCAACG GCAAGCGCCA CCACAGCACC 
CCAACCGGGG GCCGCAAGAA CAACAAGCCC ATCTACCGCA CGCCTGAGAA AGTCCGCAGA 
CACAAGAAAG TGGGCCGCAG AGACTCGCAG AGCAGCAACG AGTTCCTGAG CGCCTCCGAC 
TCCAAGGAGA ACATCAGCGT GGACTTCACC ATGACTGATG GGAGGGACAA GGACTCTCTG 
CCGGGGGAGG ACGTCCCTTT AGACCCCTTC GGGGCGCGAC CTTTCCACCC TCAGGATGGG 
GGGCGCCACG TCCAATACCC GTGTCTCAGT GAGGGTCAGA GTGACGTAGC TACAGCCAAC 
GGTAAGTCCT GGGACAGCAG GAGTATGGAT GATTTTGGAG CAGTGCCCTT CACGGAAATT 
GTCATCGGGG GACCGCAGCA GGCCCCCCCC CAGCCGCTGG ACCTGGACCC GTTCGGAGCT 
GCTCCCTTTC CTTCAAAACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024116045.1
CDS	746..4009
Translation	MKKFSRMPKSESGGLGGVSGSGLSSYFGKVFAVGRYQVTVEELVAEGGFSVVFMARTHSGVRCALKRMYVNNVQDLNICKREITIMKELSGHKNIVTYLDSTISSFSDNVWEVLILMEFCKAGQVVKQMNQRLNVGFSEAEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLNDQGNYVLCDFGSATHKVLLPHKDGVTAVEDEIKKYTTLSYRAPEMINLYAGKGITTKADIWALGCLLYKLCFFTLPFGESQVAICDGSFIVPDNSKFSCKLHCLIRYMLEPDLEKRPDIYQVSHFAFKIAGRDNPVPNLCSSPIPTSLPEPLTASEVAAKKNMTKARITDSVVPTETSIAPRQRPKAANSNVLPLTNTVTPVKMTVPSTAISNGQKVPTPSSGQPSIQAQPSSQQNRVLQQLQPGDLRLQQLQQQQGVQQQHANVQQLQYLQYQQAVQQSLQQQQQVLLQQQQMMMHPMYQQQVAQAQYAAMLHQYQQSFVQQQQQQYHPAQQPLPYMSSPLDFQIPLGPYNATTPTAGAAQMGGPSPLDPSYSTPSSSRNPLVVSDGIAPPSQTGGAISNPPDMSSWNPFGEDNFSKLTEEELLDREFDLLRAKKPAERTASTDADQPPPPNKPPPSEDLFGSIPFVANTGAAVMKGEAPAVEAGVCPPTSTALAKGPKLGKKSGCRPGEDSDSDFESDPPSPKSSEDEEQEEEEALNSEHSQDTEPENLGQRPLLMDSEEEGDKRSSDSESEPPRTKNRSRKPAGSKGGGAAAAGRSTPEASVAAPVTPHPALLRGTRASEAVDMFGALPFVGGTSPVGASQSSAVFAPFGQDEELAAEEFDVFIKAPFSRNPSKTGRSSNEAPLSPDSIDVFGFSPFQPGPVGPPPSTSRSAENIFHLQEPASPRQQRLKQRSLQKLSLRQRKTKQEAAGVGGNGKRHHSTPTGGRKNNKPIYRTPEKVRRHKKVGRRDSQSSNEFLSASDSKENISVDFTMTDGRDKDSLPGEDVPLDPFGARPFHPQDGGRHVQYPCLSEGQSDVATANGKSWDSRSMDDFGAVPFTEIVIGGPQQAPPQPLDLDPFGAAPFPSKH

Target ORF information:

RefSeq Version	XM_024260277.1
Organism	Oryzias melastigma(Indian medaka)
Definition	Oryzias melastigma BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024260277.1

ORF Insert Sequence:

ATGAAGAAGT TTTCCAGGAT GCCAAAGTCC GAAAGCGGCG GGCTCGGAGG GGTGTCCGGC 
TCCGGGCTGA GCAGCTACTT CGGGAAAGTG TTCGCGGTGG GCCGGTACCA GGTCACGGTG 
GAGGAGCTCG TCGCAGAGGG AGGCTTCTCT GTGGTGTTTA TGGCTCGAAC ACACAGTGGT 
GTCCGCTGCG CTCTCAAGAG GATGTACGTG AACAATGTGC AAGACCTGAA CATTTGCAAG 
AGAGAGATCA CTATCATGAA AGAGCTGTCA GGTCACAAGA ACATCGTCAC CTACCTGGAC 
TCCACCATCA GTTCTTTTTC AGACAATGTT TGGGAGGTCC TTATCCTCAT GGAGTTCTGC 
AAAGCGGGTC AGGTGGTGAA ACAGATGAAC CAGAGGTTAA ATGTGGGCTT CAGTGAAGCT 
GAAGTCCTGC AGATTTTCTG CGACACCTGC GAGGCCGTGG CTCGCCTGCA CCAATGCAAG 
ACCCCCATCA TCCACAGAGA CCTGAAAGTG GAGAACATTC TGCTGAATGA CCAGGGGAAC 
TATGTGCTGT GTGACTTTGG CAGTGCCACT CACAAAGTCT TACTGCCTCA CAAGGATGGA 
GTGACTGCTG TGGAGGATGA AATCAAAAAG TACACGACTC TTTCATATCG GGCTCCAGAG 
ATGATTAACT TGTATGCAGG AAAAGGCATC ACCACTAAGG CTGATATTTG GGCTCTGGGA 
TGCTTGCTGT ACAAGCTGTG TTTCTTCACT CTTCCTTTTG GAGAGAGTCA AGTTGCCATT 
TGTGACGGGA GCTTCATTGT TCCAGATAAC TCCAAGTTCT CTTGCAAACT GCACTGCTTG 
ATCAGATATA TGCTGGAGCC AGACCTGGAG AAGAGACCAG ACATTTACCA AGTGTCCCAC 
TTTGCCTTTA AGATAGCTGG AAGAGATAAT CCTGTACCAA ACCTCTGTAG CTCTCCCATC 
CCTACATCAC TCCCTGAGCC GCTCACAGCC AGCGAGGTTG CTGCCAAAAA GAATATGACA 
AAAGCCAGGA TTACAGACTC TGTTGTTCCA ACTGAGACCT CTATAGCTCC CAGGCAGAGA 
CCAAAGGCTG CAAACTCCAA CGTTCTCCCC CTCACTAACA CTGTCACCCC CGTCAAGATG 
ACCGTGCCTT CCACAGCCAT CAGCAATGGC CAGAAAGTCC CTACTCCCAG CTCTGGACAG 
CCATCCATAC AGGCCCAGCC CAGCAGTCAG CAGAACCGAG TCCTGCAGCA GCTGCAGCCA 
GGTGATCTGC GTTTGCAGCA GCTGCAGCAA CAACAAGGAG TGCAGCAGCA GCATGCAAAT 
GTGCAGCAAC TCCAGTACCT TCAGTATCAA CAGGCAGTGC AGCAGTCCCT CCAGCAGCAG 
CAGCAAGTCT TGCTCCAGCA GCAGCAGATG ATGATGCATC CCATGTACCA GCAGCAGGTT 
GCACAGGCCC AGTATGCTGC TATGCTGCAC CAGTACCAAC AGTCCTTTGT CCAGCAGCAG 
CAGCAGCAGT ATCATCCCGC TCAGCAGCCT CTCCCCTACA TGTCTTCCCC TCTTGACTTC 
CAGATCCCTC TGGGGCCCTA CAATGCCACT ACTCCCACTG CTGGGGCTGC TCAGATGGGA 
GGACCATCAC CTCTGGATCC TTCATACTCC ACCCCCAGCA GCAGTAGAAA CCCCCTGGTG 
GTCTCAGATG GAATCGCCCC TCCTTCTCAG ACCGGCGGCG CCATCAGTAA CCCTCCCGAC 
ATGTCGAGCT GGAACCCGTT TGGAGAGGAC AACTTCTCCA AACTAACGGA GGAAGAGCTG 
CTGGACCGGG AGTTTGACCT GCTCAGAGCA AAAAAGCCGG CAGAGAGGAC AGCCAGCACG 
GACGCTGACC AACCGCCACC TCCCAACAAG CCCCCCCCAT CAGAGGACCT GTTTGGCTCC 
ATCCCTTTTG TGGCCAACAC AGGTGCTGCT GTGATGAAAG GTGAGGCACC TGCTGTGGAG 
GCTGGCGTCT GCCCCCCCAC CTCCACAGCC CTAGCTAAGG GGCCCAAACT GGGCAAAAAG 
AGCGGCTGCA GACCTGGAGA AGACTCAGAC AGCGACTTTG AGTCGGATCC TCCTTCTCCA 
AAGAGCAGTG AGGATGAGGA GCAGGAGGAA GAGGAGGCCC TGAACAGTGA GCACAGCCAA 
GACACTGAGC CAGAGAACTT GGGACAGAGA CCTTTGCTGA TGGACTCTGA GGAGGAGGGG 
GACAAGCGCA GCTCTGATTC AGAGTCTGAG CCTCCCAGGA CAAAGAACCG ATCCAGGAAG 
CCTGCAGGGA GCAAAGGAGG AGGAGCAGCT GCTGCCGGCA GGAGCACTCC AGAAGCCTCT 
GTGGCTGCTC CTGTCACCCC CCATCCTGCG TTGCTCAGAG GAACCAGAGC CTCAGAGGCT 
GTAGATATGT TTGGCGCCTT GCCCTTTGTG GGGGGCACCT CCCCTGTGGG GGCCTCACAG 
AGTTCTGCTG TGTTTGCACC TTTCGGGCAG GATGAGGAGC TGGCTGCAGA GGAGTTTGAT 
GTTTTCATCA AAGCTCCCTT CAGCAGGAAC CCCTCAAAGA CGGGAAGAAG CAGCAATGAA 
GCCCCCCTGT CCCCCGATTC CATTGACGTG TTTGGATTCT CTCCTTTCCA GCCGGGCCCT 
GTTGGACCAC CTCCAAGCAC TTCCAGGAGC GCCGAAAACA TCTTCCACCT CCAGGAGCCT 
GCCAGTCCCC GGCAGCAGAG GCTGAAGCAG CGCAGCCTGC AGAAGCTGTC CTTGCGCCAG 
AGGAAGACCA AGCAGGAGGC AGCCGGGGTA GGCGGCAACG GCAAGCGCCA CCACAGCACC 
CCAACCGGGG GCCGCAAGAA CAACAAGCCC ATCTACCGCA CGCCTGAGAA AGTCCGCAGA 
CACAAGAAAG TGGGCCGCAG AGACTCGCAG AGCAGCAACG AGTTCCTGAG CGCCTCCGAC 
TCCAAGGAGA ACATCAGCGT GGACTTCACC ATGACTGATG GGAGGGACAA GGACTCTCTG 
CCGGGGGAGG ACGTCCCTTT AGACCCCTTC GGGGCGCGAC CTTTCCACCC TCAGGATGGG 
GGGCGCCACG TCCAATACCC GTGTCTCAGT GAGGGTCAGA GTGACGTAGC TACAGCCAAC 
GGTAAGTCCT GGGACAGCAG GAGTATGGAT GATTTTGGAG CAGTGCCCTT CACGGAAATT 
GTCATCGGGG GACCGCAGCA GGCCCCCCCC CAGCCGCTGG ACCTGGACCC GTTCGGAGCT 
GCTCCCTTTC CTTCAAAACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bmp2k ( XM_024260278.1 ) cDNA ORF clone, -> XP_024116046.1 Oryzias melastigma BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIn32267
Clone ID Related Accession (Same CDS sequence)	XM_024260278.1
Accession Version	XM_024260278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-10
Organism	Oryzias melastigma(Indian medaka)
Product	BMP-2-inducible protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020096109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias melastigma Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTCCAGGAT GCCAAAGTCC GAAAGCGGCG GGCTCGGAGG GGTGTCCGGC 
TCCGGGCTGA GCAGCTACTT CGGGAAAGTG TTCGCGGTGG GCCGGTACCA GGTCACGGTG 
GAGGAGCTCG TCGCAGAGGG AGGCTTCTCT GTGGTGTTTA TGGCTCGAAC ACACAGTGGT 
GTCCGCTGCG CTCTCAAGAG GATGTACGTG AACAATGTGC AAGACCTGAA CATTTGCAAG 
AGAGAGATCA CTATCATGAA AGAGCTGTCA GGTCACAAGA ACATCGTCAC CTACCTGGAC 
TCCACCATCA GTTCTTTTTC AGACAATGTT TGGGAGGTCC TTATCCTCAT GGAGTTCTGC 
AAAGCGGGTC AGGTGGTGAA ACAGATGAAC CAGAGGTTAA ATGTGGGCTT CAGTGAAGCT 
GAAGTCCTGC AGATTTTCTG CGACACCTGC GAGGCCGTGG CTCGCCTGCA CCAATGCAAG 
ACCCCCATCA TCCACAGAGA CCTGAAAGTG GAGAACATTC TGCTGAATGA CCAGGGGAAC 
TATGTGCTGT GTGACTTTGG CAGTGCCACT CACAAAGTCT TACTGCCTCA CAAGGATGGA 
GTGACTGCTG TGGAGGATGA AATCAAAAAG TACACGACTC TTTCATATCG GGCTCCAGAG 
ATGATTAACT TGTATGCAGG AAAAGGCATC ACCACTAAGG CTGATATTTG GGCTCTGGGA 
TGCTTGCTGT ACAAGCTGTG TTTCTTCACT CTTCCTTTTG GAGAGAGTCA AGTTGCCATT 
TGTGACGGGA GCTTCATTGT TCCAGATAAC TCCAAGTTCT CTTGCAAACT GCACTGCTTG 
ATCAGATATA TGCTGGAGCC AGACCTGGAG AAGAGACCAG ACATTTACCA AGTGTCCCAC 
TTTGCCTTTA AGATAGCTGG AAGAGATAAT CCTGTACCAA ACCTCTGTAG CTCTCCCATC 
CCTACATCAC TCCCTGAGCC GCTCACAGCC AGCGAGGTTG CTGCCAAAAA GAATATGACA 
AAAGCCAGGA TTACAGACTC TGTTGTTCCA ACTGAGACCT CTATAGCTCC CAGGCAGAGA 
CCAAAGGCTG CAAACTCCAA CGTTCTCCCC CTCACTAACA CTGTCACCCC CGTCAAGATG 
ACCGTGCCTT CCACAGCCAT CAGCAATGGC CAGAAAGTCC CTACTCCCAG CTCTGGACAG 
CCATCCATAC AGGCCCAGCC CAGCAGTCAG CAGAACCGAG TCCTGCAGCA GCTGCAGCCA 
GGTGATCTGC GTTTGCAGCA GCTGCAGCAA CAACAAGGAG TGCAGCAGCA GCATGCAAAT 
GTGCAGCAAC TCCAGTACCT TCAGTATCAA CAGGCAGTGC AGCAGTCCCT CCAGCAGCAG 
CAGCAAGTCT TGCTCCAGCA GCAGCAGATG ATGATGCATC CCATGTACCA GCAGCAGGTT 
GCACAGGCCC AGTATGCTGC TATGCTGCAC CAGTACCAAC AGTCCTTTGT CCAGCAGCAG 
CAGCAGCAGT ATCATCCCGC TCAGCAGCCT CTCCCCTACA TGTCTTCCCC TCTTGACTTC 
CAGATCCCTC TGGGGCCCTA CAATGCCACT ACTCCCACTG CTGGGGCTGC TCAGATGGGA 
GGACCATCAC CTCTGGATCC TTCATACTCC ACCCCCAGCA GTAGAAACCC CCTGGTGGTC 
TCAGATGGAA TCGCCCCTCC TTCTCAGACC GGCGGCGCCA TCAGTAACCC TCCCGACATG 
TCGAGCTGGA ACCCGTTTGG AGAGGACAAC TTCTCCAAAC TAACGGAGGA AGAGCTGCTG 
GACCGGGAGT TTGACCTGCT CAGAGCAAAA AAGCCGGCAG AGAGGACAGC CAGCACGGAC 
GCTGACCAAC CGCCACCTCC CAACAAGCCC CCCCCATCAG AGGACCTGTT TGGCTCCATC 
CCTTTTGTGG CCAACACAGG TGCTGCTGTG ATGAAAGGTG AGGCACCTGC TGTGGAGGCT 
GGCGTCTGCC CCCCCACCTC CACAGCCCTA GCTAAGGGGC CCAAACTGGG CAAAAAGAGC 
GGCTGCAGAC CTGGAGAAGA CTCAGACAGC GACTTTGAGT CGGATCCTCC TTCTCCAAAG 
AGCAGTGAGG ATGAGGAGCA GGAGGAAGAG GAGGCCCTGA ACAGTGAGCA CAGCCAAGAC 
ACTGAGCCAG AGAACTTGGG ACAGAGACCT TTGCTGATGG ACTCTGAGGA GGAGGGGGAC 
AAGCGCAGCT CTGATTCAGA GTCTGAGCCT CCCAGGACAA AGAACCGATC CAGGAAGCCT 
GCAGGGAGCA AAGGAGGAGG AGCAGCTGCT GCCGGCAGGA GCACTCCAGA AGCCTCTGTG 
GCTGCTCCTG TCACCCCCCA TCCTGCGTTG CTCAGAGGAA CCAGAGCCTC AGAGGCTGTA 
GATATGTTTG GCGCCTTGCC CTTTGTGGGG GGCACCTCCC CTGTGGGGGC CTCACAGAGT 
TCTGCTGTGT TTGCACCTTT CGGGCAGGAT GAGGAGCTGG CTGCAGAGGA GTTTGATGTT 
TTCATCAAAG CTCCCTTCAG CAGGAACCCC TCAAAGACGG GAAGAAGCAG CAATGAAGCC 
CCCCTGTCCC CCGATTCCAT TGACGTGTTT GGATTCTCTC CTTTCCAGCC GGGCCCTGTT 
GGACCACCTC CAAGCACTTC CAGGAGCGCC GAAAACATCT TCCACCTCCA GGAGCCTGCC 
AGTCCCCGGC AGCAGAGGCT GAAGCAGCGC AGCCTGCAGA AGCTGTCCTT GCGCCAGAGG 
AAGACCAAGC AGGAGGCAGC CGGGGTAGGC GGCAACGGCA AGCGCCACCA CAGCACCCCA 
ACCGGGGGCC GCAAGAACAA CAAGCCCATC TACCGCACGC CTGAGAAAGT CCGCAGACAC 
AAGAAAGTGG GCCGCAGAGA CTCGCAGAGC AGCAACGAGT TCCTGAGCGC CTCCGACTCC 
AAGGAGAACA TCAGCGTGGA CTTCACCATG ACTGATGGGA GGGACAAGGA CTCTCTGCCG 
GGGGAGGACG TCCCTTTAGA CCCCTTCGGG GCGCGACCTT TCCACCCTCA GGATGGGGGG 
CGCCACGTCC AATACCCGTG TCTCAGTGAG GGTCAGAGTG ACGTAGCTAC AGCCAACGGT 
AAGTCCTGGG ACAGCAGGAG TATGGATGAT TTTGGAGCAG TGCCCTTCAC GGAAATTGTC 
ATCGGGGGAC CGCAGCAGGC CCCCCCCCAG CCGCTGGACC TGGACCCGTT CGGAGCTGCT 
CCCTTTCCTT CAAAACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024116046.1
CDS	745..4005
Translation	MKKFSRMPKSESGGLGGVSGSGLSSYFGKVFAVGRYQVTVEELVAEGGFSVVFMARTHSGVRCALKRMYVNNVQDLNICKREITIMKELSGHKNIVTYLDSTISSFSDNVWEVLILMEFCKAGQVVKQMNQRLNVGFSEAEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLNDQGNYVLCDFGSATHKVLLPHKDGVTAVEDEIKKYTTLSYRAPEMINLYAGKGITTKADIWALGCLLYKLCFFTLPFGESQVAICDGSFIVPDNSKFSCKLHCLIRYMLEPDLEKRPDIYQVSHFAFKIAGRDNPVPNLCSSPIPTSLPEPLTASEVAAKKNMTKARITDSVVPTETSIAPRQRPKAANSNVLPLTNTVTPVKMTVPSTAISNGQKVPTPSSGQPSIQAQPSSQQNRVLQQLQPGDLRLQQLQQQQGVQQQHANVQQLQYLQYQQAVQQSLQQQQQVLLQQQQMMMHPMYQQQVAQAQYAAMLHQYQQSFVQQQQQQYHPAQQPLPYMSSPLDFQIPLGPYNATTPTAGAAQMGGPSPLDPSYSTPSSRNPLVVSDGIAPPSQTGGAISNPPDMSSWNPFGEDNFSKLTEEELLDREFDLLRAKKPAERTASTDADQPPPPNKPPPSEDLFGSIPFVANTGAAVMKGEAPAVEAGVCPPTSTALAKGPKLGKKSGCRPGEDSDSDFESDPPSPKSSEDEEQEEEEALNSEHSQDTEPENLGQRPLLMDSEEEGDKRSSDSESEPPRTKNRSRKPAGSKGGGAAAAGRSTPEASVAAPVTPHPALLRGTRASEAVDMFGALPFVGGTSPVGASQSSAVFAPFGQDEELAAEEFDVFIKAPFSRNPSKTGRSSNEAPLSPDSIDVFGFSPFQPGPVGPPPSTSRSAENIFHLQEPASPRQQRLKQRSLQKLSLRQRKTKQEAAGVGGNGKRHHSTPTGGRKNNKPIYRTPEKVRRHKKVGRRDSQSSNEFLSASDSKENISVDFTMTDGRDKDSLPGEDVPLDPFGARPFHPQDGGRHVQYPCLSEGQSDVATANGKSWDSRSMDDFGAVPFTEIVIGGPQQAPPQPLDLDPFGAAPFPSKH

Target ORF information:

RefSeq Version	XM_024260278.1
Organism	Oryzias melastigma(Indian medaka)
Definition	Oryzias melastigma BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024260278.1

ORF Insert Sequence:

ATGAAGAAGT TTTCCAGGAT GCCAAAGTCC GAAAGCGGCG GGCTCGGAGG GGTGTCCGGC 
TCCGGGCTGA GCAGCTACTT CGGGAAAGTG TTCGCGGTGG GCCGGTACCA GGTCACGGTG 
GAGGAGCTCG TCGCAGAGGG AGGCTTCTCT GTGGTGTTTA TGGCTCGAAC ACACAGTGGT 
GTCCGCTGCG CTCTCAAGAG GATGTACGTG AACAATGTGC AAGACCTGAA CATTTGCAAG 
AGAGAGATCA CTATCATGAA AGAGCTGTCA GGTCACAAGA ACATCGTCAC CTACCTGGAC 
TCCACCATCA GTTCTTTTTC AGACAATGTT TGGGAGGTCC TTATCCTCAT GGAGTTCTGC 
AAAGCGGGTC AGGTGGTGAA ACAGATGAAC CAGAGGTTAA ATGTGGGCTT CAGTGAAGCT 
GAAGTCCTGC AGATTTTCTG CGACACCTGC GAGGCCGTGG CTCGCCTGCA CCAATGCAAG 
ACCCCCATCA TCCACAGAGA CCTGAAAGTG GAGAACATTC TGCTGAATGA CCAGGGGAAC 
TATGTGCTGT GTGACTTTGG CAGTGCCACT CACAAAGTCT TACTGCCTCA CAAGGATGGA 
GTGACTGCTG TGGAGGATGA AATCAAAAAG TACACGACTC TTTCATATCG GGCTCCAGAG 
ATGATTAACT TGTATGCAGG AAAAGGCATC ACCACTAAGG CTGATATTTG GGCTCTGGGA 
TGCTTGCTGT ACAAGCTGTG TTTCTTCACT CTTCCTTTTG GAGAGAGTCA AGTTGCCATT 
TGTGACGGGA GCTTCATTGT TCCAGATAAC TCCAAGTTCT CTTGCAAACT GCACTGCTTG 
ATCAGATATA TGCTGGAGCC AGACCTGGAG AAGAGACCAG ACATTTACCA AGTGTCCCAC 
TTTGCCTTTA AGATAGCTGG AAGAGATAAT CCTGTACCAA ACCTCTGTAG CTCTCCCATC 
CCTACATCAC TCCCTGAGCC GCTCACAGCC AGCGAGGTTG CTGCCAAAAA GAATATGACA 
AAAGCCAGGA TTACAGACTC TGTTGTTCCA ACTGAGACCT CTATAGCTCC CAGGCAGAGA 
CCAAAGGCTG CAAACTCCAA CGTTCTCCCC CTCACTAACA CTGTCACCCC CGTCAAGATG 
ACCGTGCCTT CCACAGCCAT CAGCAATGGC CAGAAAGTCC CTACTCCCAG CTCTGGACAG 
CCATCCATAC AGGCCCAGCC CAGCAGTCAG CAGAACCGAG TCCTGCAGCA GCTGCAGCCA 
GGTGATCTGC GTTTGCAGCA GCTGCAGCAA CAACAAGGAG TGCAGCAGCA GCATGCAAAT 
GTGCAGCAAC TCCAGTACCT TCAGTATCAA CAGGCAGTGC AGCAGTCCCT CCAGCAGCAG 
CAGCAAGTCT TGCTCCAGCA GCAGCAGATG ATGATGCATC CCATGTACCA GCAGCAGGTT 
GCACAGGCCC AGTATGCTGC TATGCTGCAC CAGTACCAAC AGTCCTTTGT CCAGCAGCAG 
CAGCAGCAGT ATCATCCCGC TCAGCAGCCT CTCCCCTACA TGTCTTCCCC TCTTGACTTC 
CAGATCCCTC TGGGGCCCTA CAATGCCACT ACTCCCACTG CTGGGGCTGC TCAGATGGGA 
GGACCATCAC CTCTGGATCC TTCATACTCC ACCCCCAGCA GTAGAAACCC CCTGGTGGTC 
TCAGATGGAA TCGCCCCTCC TTCTCAGACC GGCGGCGCCA TCAGTAACCC TCCCGACATG 
TCGAGCTGGA ACCCGTTTGG AGAGGACAAC TTCTCCAAAC TAACGGAGGA AGAGCTGCTG 
GACCGGGAGT TTGACCTGCT CAGAGCAAAA AAGCCGGCAG AGAGGACAGC CAGCACGGAC 
GCTGACCAAC CGCCACCTCC CAACAAGCCC CCCCCATCAG AGGACCTGTT TGGCTCCATC 
CCTTTTGTGG CCAACACAGG TGCTGCTGTG ATGAAAGGTG AGGCACCTGC TGTGGAGGCT 
GGCGTCTGCC CCCCCACCTC CACAGCCCTA GCTAAGGGGC CCAAACTGGG CAAAAAGAGC 
GGCTGCAGAC CTGGAGAAGA CTCAGACAGC GACTTTGAGT CGGATCCTCC TTCTCCAAAG 
AGCAGTGAGG ATGAGGAGCA GGAGGAAGAG GAGGCCCTGA ACAGTGAGCA CAGCCAAGAC 
ACTGAGCCAG AGAACTTGGG ACAGAGACCT TTGCTGATGG ACTCTGAGGA GGAGGGGGAC 
AAGCGCAGCT CTGATTCAGA GTCTGAGCCT CCCAGGACAA AGAACCGATC CAGGAAGCCT 
GCAGGGAGCA AAGGAGGAGG AGCAGCTGCT GCCGGCAGGA GCACTCCAGA AGCCTCTGTG 
GCTGCTCCTG TCACCCCCCA TCCTGCGTTG CTCAGAGGAA CCAGAGCCTC AGAGGCTGTA 
GATATGTTTG GCGCCTTGCC CTTTGTGGGG GGCACCTCCC CTGTGGGGGC CTCACAGAGT 
TCTGCTGTGT TTGCACCTTT CGGGCAGGAT GAGGAGCTGG CTGCAGAGGA GTTTGATGTT 
TTCATCAAAG CTCCCTTCAG CAGGAACCCC TCAAAGACGG GAAGAAGCAG CAATGAAGCC 
CCCCTGTCCC CCGATTCCAT TGACGTGTTT GGATTCTCTC CTTTCCAGCC GGGCCCTGTT 
GGACCACCTC CAAGCACTTC CAGGAGCGCC GAAAACATCT TCCACCTCCA GGAGCCTGCC 
AGTCCCCGGC AGCAGAGGCT GAAGCAGCGC AGCCTGCAGA AGCTGTCCTT GCGCCAGAGG 
AAGACCAAGC AGGAGGCAGC CGGGGTAGGC GGCAACGGCA AGCGCCACCA CAGCACCCCA 
ACCGGGGGCC GCAAGAACAA CAAGCCCATC TACCGCACGC CTGAGAAAGT CCGCAGACAC 
AAGAAAGTGG GCCGCAGAGA CTCGCAGAGC AGCAACGAGT TCCTGAGCGC CTCCGACTCC 
AAGGAGAACA TCAGCGTGGA CTTCACCATG ACTGATGGGA GGGACAAGGA CTCTCTGCCG 
GGGGAGGACG TCCCTTTAGA CCCCTTCGGG GCGCGACCTT TCCACCCTCA GGATGGGGGG 
CGCCACGTCC AATACCCGTG TCTCAGTGAG GGTCAGAGTG ACGTAGCTAC AGCCAACGGT 
AAGTCCTGGG ACAGCAGGAG TATGGATGAT TTTGGAGCAG TGCCCTTCAC GGAAATTGTC 
ATCGGGGGAC CGCAGCAGGC CCCCCCCCAG CCGCTGGACC TGGACCCGTT CGGAGCTGCT 
CCCTTTCCTT CAAAACACTG A