sik2 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	sik2
Entrez Gene ID	101167310
Full Name	salt inducible kinase 2
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004075670.3	XP_004075718.1	serine/threonine-protein kinase SIK2
XM_004075670.4	XP_004075718.1	serine/threonine-protein kinase SIK2

Pathways from BioSystems
Gene Ontology

The following sik2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sik2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa09997	XM_004075670.4 Latest version!	Oryzias latipes salt inducible kinase 2 (sik2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OJa09997	XM_004075670.3	Oryzias latipes salt inducible kinase 2 (sik2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sik2 ( XM_004075670.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_004075718.1 Oryzias latipes salt inducible kinase 2 (sik2), mRNA.

Price & Availability↑

CloneID	OJa09997
Clone ID Related Accession (Same CDS sequence)	XM_004075670.3 , XM_004075670.4
Accession Version	XM_004075670.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-06
Organism	Oryzias latipes(Japanese medaka)
Product	serine/threonine-protein kinase SIK2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_004075670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCATGG CTGACGGTGT CCAAAAGCCG CTGACCCGGG GTCCGGTCCG AGTGGGCTTC 
TACGACATCG AACGCACTTT AGGAAAGGGC AATTTCGCCG TGGTGAAATT GGCTAGACAC 
CGAATAACCA AGACCGAGGT GGCCATTAAG ATCATTGACA AGACCCAACT AGACGCTGTA 
AACCTGGAGA AGATCTACAG GGAAGTTCAA ATCATGAAGA TGTTGGACCA CCCTCACATC 
ATTAAGCTTT ACCAGGTGAT GGAAACAAAG AACATGCTTT ACTTAGTCAC GGAGTATGCC 
AAGAGCGGGG AGATCTTCGA CTATCTGGCA AAGCATGGGC GTCTCAGTGA GCTGGAGGCC 
AGGAGGAAGT TTTGGCAGAT TCTGTCTGCA GTCGAGTACT GTCACAACCG GAACATTGTC 
CACAGAGACC TGAAGGCTGA AAATTTGCTG TTGGACGGTC ACATGAACAT CAAGATTGCA 
GATTTTGGAT TTGGAAACTT CTTTAAACAA GGAGAGCCTT TGGCTACATG GTGTGGCAGC 
CCGCCATACG CTGCTCCAGA GGTCTTTGAA GGACAACAGT ATGAGGGCCC ACAGCTGGAC 
ATCTGGAGTA TGGGGGTGGT GCTTTATGTG CTGGTGTGCG GGGCGTTGCC CTTTGACGGG 
CCCAGTCTGC CTGTCCTCCG ACAGAGAGTC CTAGAAGGAA GGTTCCGCAT CCCATACTTC 
ATGACTGAAG ACTGTGAACA CTTGATCCGC AGGATGTTGG TCCTGGACCC GTCCAAACGC 
CTGTCCTTGG CACAGATTAA GGAGCACAAA TGGATGGCTT TGGACGTCCC AGCACAGAGG 
CCCGTCCTGT ACCAACAGCC ACTGTCAGCT GAGGGTGAGA TGGGTGTAGG AGAATACAGC 
GAACAGGTCC TTCGCCTGAT GCACAGCCTT GGCATCGACC AACAAAAGAC TATAGAGTCT 
CTTCAGAATA AAAGCTACAA CCACTTCGCT GCGATCTACT ACCTGCTGGT CGAGCGGCTG 
AAGGCCCATC GATGCAGCTT CCCTGTGGAA CAAAGGCTCG ACGCCCGCCA GCGGCGCCCC 
AGCACCATCG CTGAGCAGAC GGTCATCAAG TCAACCAGCG GCTCAGCCCA GGTGGGTTTG 
CTTCCACAGG GCGTTCGTCT GTTGCGTTCC CCCGCGGTTC CTCAGCCCTC GTCGGATGCT 
TTTACCTTCC CCCAGACTGC ATGTCCCCTG GAGCAGAACT TCCTTGTGGA GGATGTCAGC 
ACACCCAAAG TGAATGGCTG CCTTCTGGAC CCCCTTCCTC CCTCTACTGT CCTCCGCAAG 
TCCTCCACTT CGTCACCTAG CAACATGATG GAGACCTCGA TTGACGAAGG CATTGAGACT 
GAGGAGCCCG ACACGGAGGA CGACACGCCC CACCTTCTCT CAGCCTATCA GACGACTCGA 
TTTGGCCAGA GGCGGCACAC CTTATCTGAA GTTTCCAACC CGCCTGGGCC TTCAAGCCAA 
GGTAAATATT TCACTTTAGG CCACAACACC TCCATGGGCA GCGTGGACTC GGACATTGGC 
TACGATATGG GCTCCGTGCA CAGCGACCTC AGTCTGCTGG AGGACGCGCC CTCCCTCAGT 
GAGGTGGTAC CATCCAACAG CTCTGCTCCT GGCATAGCTG CACCTTTTCT GAGTGCCAGG 
CCACCCAACC CAGCAATGGC TGCCCTGACT TCTCAGCACA GGGAGGCGCA CAACCGATCC 
CCGATAAGCT TCCGAGAAGG ACGCCGCGCC TCTGACACCT CCCTCACCCA AGGCCTGGTG 
GCGTTTCGGC AGCACCTCCA AAACTTGGCA AGGACCAAAG GCATCCTGGA CCTCAACAAA 
GTCCAGATGC TGGTGGAGCA GATGGGCTCT GTGGAAGGGG CCAGCATGGG GCCTCTGGGA 
CCACAGCAAC ACTTGCACAA CCTCCTGGAG GGCGAGGCAT GCAAGCAGCA GGAGGGCCTA 
GCTTTATACC ACGGTGGGCT GCAGCCCCCC CTTCTCTCCC GCAGACAGAG TTTGGAAACA 
CAGTACCTCA CCCACAGACT TCAGGCTAAC ATCCTGGCTA ACAGCCCTGC GAGCTGCCAA 
CTTTTCTGCA AGGAGACGCC GAGAAGTTTA GAGCAGCAGC TACAGGAACA CAGGCTGCAT 
CAGAAGAGGA TGTATCTGCA GCAGCACCAG TCACAGGGCA TGGGACTCCA GGCCTACTTC 
AACCAGATGC AGATAGCTGA GGGCTCCTAC GCCCCAAGCG GCCCCATCCT GGATCCATCA 
GCCGCCCAGA ACTCCCCCCA GGCCCCCTCC ATGTCGGCCC CCCACCAGCC TCAGTCCCAG 
CAGCAGGGCA GTCCCCAAGC CTCACCCCCT TTCAGCCACA CCCACAGCTT GAGCCCTTTG 
ATGGAACCCG GCGAGGGCCT GGTTTACGAC CCGTACATGA GCCACCACCA CCCTTACACC 
CAACAGCTGC CCCCCGGAGC TCACCTCATG CACCACCCAC TCCACCATCA GCATTCTCTG 
GAGGCCCCTG TTGGCGGCTT GGGCTTCAGC TACCCAGCAG GCTGTGATCC ACATTCCCCT 
GGTCCTTCCG CTGAGGCTCT CCTGCCAGAG CAATACGAGT TCTCCATGGA CGCCAGCCTG 
CTGCCTCAGC CTGGTCCAGG GGAGGCAGAC GCTGGAGGCC CCGTGGCAGC GCAGACAGAC 
GGCTTAGGCA TGCCTAAACT GCAGGCGTCC CTCCTGGACA GTGAAATGAT GGAGACCGTG 
GACTCGCAGC ATGGCTTCGT GCTGGTCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004075718.1
CDS	380..3172
Translation	MVMADGVQKPLTRGPVRVGFYDIERTLGKGNFAVVKLARHRITKTEVAIKIIDKTQLDAVNLEKIYREVQIMKMLDHPHIIKLYQVMETKNMLYLVTEYAKSGEIFDYLAKHGRLSELEARRKFWQILSAVEYCHNRNIVHRDLKAENLLLDGHMNIKIADFGFGNFFKQGEPLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPSLPVLRQRVLEGRFRIPYFMTEDCEHLIRRMLVLDPSKRLSLAQIKEHKWMALDVPAQRPVLYQQPLSAEGEMGVGEYSEQVLRLMHSLGIDQQKTIESLQNKSYNHFAAIYYLLVERLKAHRCSFPVEQRLDARQRRPSTIAEQTVIKSTSGSAQVGLLPQGVRLLRSPAVPQPSSDAFTFPQTACPLEQNFLVEDVSTPKVNGCLLDPLPPSTVLRKSSTSSPSNMMETSIDEGIETEEPDTEDDTPHLLSAYQTTRFGQRRHTLSEVSNPPGPSSQGKYFTLGHNTSMGSVDSDIGYDMGSVHSDLSLLEDAPSLSEVVPSNSSAPGIAAPFLSARPPNPAMAALTSQHREAHNRSPISFREGRRASDTSLTQGLVAFRQHLQNLARTKGILDLNKVQMLVEQMGSVEGASMGPLGPQQHLHNLLEGEACKQQEGLALYHGGLQPPLLSRRQSLETQYLTHRLQANILANSPASCQLFCKETPRSLEQQLQEHRLHQKRMYLQQHQSQGMGLQAYFNQMQIAEGSYAPSGPILDPSAAQNSPQAPSMSAPHQPQSQQQGSPQASPPFSHTHSLSPLMEPGEGLVYDPYMSHHHPYTQQLPPGAHLMHHPLHHQHSLEAPVGGLGFSYPAGCDPHSPGPSAEALLPEQYEFSMDASLLPQPGPGEADAGGPVAAQTDGLGMPKLQASLLDSEMMETVDSQHGFVLVN

Target ORF information:

RefSeq Version	XM_004075670.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes salt inducible kinase 2 (sik2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004075670.3

ORF Insert Sequence:

ATGGTCATGG CTGACGGTGT CCAAAAGCCG CTGACCCGGG GTCCGGTCCG AGTGGGCTTC 
TACGACATCG AACGCACTTT AGGAAAGGGC AATTTCGCCG TGGTGAAATT GGCTAGACAC 
CGAATAACCA AGACCGAGGT GGCCATTAAG ATCATTGACA AGACCCAACT AGACGCTGTA 
AACCTGGAGA AGATCTACAG GGAAGTTCAA ATCATGAAGA TGTTGGACCA CCCTCACATC 
ATTAAGCTTT ACCAGGTGAT GGAAACAAAG AACATGCTTT ACTTAGTCAC GGAGTATGCC 
AAGAGCGGGG AGATCTTCGA CTATCTGGCA AAGCATGGGC GTCTCAGTGA GCTGGAGGCC 
AGGAGGAAGT TTTGGCAGAT TCTGTCTGCA GTCGAGTACT GTCACAACCG GAACATTGTC 
CACAGAGACC TGAAGGCTGA AAATTTGCTG TTGGACGGTC ACATGAACAT CAAGATTGCA 
GATTTTGGAT TTGGAAACTT CTTTAAACAA GGAGAGCCTT TGGCTACATG GTGTGGCAGC 
CCGCCATACG CTGCTCCAGA GGTCTTTGAA GGACAACAGT ATGAGGGCCC ACAGCTGGAC 
ATCTGGAGTA TGGGGGTGGT GCTTTATGTG CTGGTGTGCG GGGCGTTGCC CTTTGACGGG 
CCCAGTCTGC CTGTCCTCCG ACAGAGAGTC CTAGAAGGAA GGTTCCGCAT CCCATACTTC 
ATGACTGAAG ACTGTGAACA CTTGATCCGC AGGATGTTGG TCCTGGACCC GTCCAAACGC 
CTGTCCTTGG CACAGATTAA GGAGCACAAA TGGATGGCTT TGGACGTCCC AGCACAGAGG 
CCCGTCCTGT ACCAACAGCC ACTGTCAGCT GAGGGTGAGA TGGGTGTAGG AGAATACAGC 
GAACAGGTCC TTCGCCTGAT GCACAGCCTT GGCATCGACC AACAAAAGAC TATAGAGTCT 
CTTCAGAATA AAAGCTACAA CCACTTCGCT GCGATCTACT ACCTGCTGGT CGAGCGGCTG 
AAGGCCCATC GATGCAGCTT CCCTGTGGAA CAAAGGCTCG ACGCCCGCCA GCGGCGCCCC 
AGCACCATCG CTGAGCAGAC GGTCATCAAG TCAACCAGCG GCTCAGCCCA GGTGGGTTTG 
CTTCCACAGG GCGTTCGTCT GTTGCGTTCC CCCGCGGTTC CTCAGCCCTC GTCGGATGCT 
TTTACCTTCC CCCAGACTGC ATGTCCCCTG GAGCAGAACT TCCTTGTGGA GGATGTCAGC 
ACACCCAAAG TGAATGGCTG CCTTCTGGAC CCCCTTCCTC CCTCTACTGT CCTCCGCAAG 
TCCTCCACTT CGTCACCTAG CAACATGATG GAGACCTCGA TTGACGAAGG CATTGAGACT 
GAGGAGCCCG ACACGGAGGA CGACACGCCC CACCTTCTCT CAGCCTATCA GACGACTCGA 
TTTGGCCAGA GGCGGCACAC CTTATCTGAA GTTTCCAACC CGCCTGGGCC TTCAAGCCAA 
GGTAAATATT TCACTTTAGG CCACAACACC TCCATGGGCA GCGTGGACTC GGACATTGGC 
TACGATATGG GCTCCGTGCA CAGCGACCTC AGTCTGCTGG AGGACGCGCC CTCCCTCAGT 
GAGGTGGTAC CATCCAACAG CTCTGCTCCT GGCATAGCTG CACCTTTTCT GAGTGCCAGG 
CCACCCAACC CAGCAATGGC TGCCCTGACT TCTCAGCACA GGGAGGCGCA CAACCGATCC 
CCGATAAGCT TCCGAGAAGG ACGCCGCGCC TCTGACACCT CCCTCACCCA AGGCCTGGTG 
GCGTTTCGGC AGCACCTCCA AAACTTGGCA AGGACCAAAG GCATCCTGGA CCTCAACAAA 
GTCCAGATGC TGGTGGAGCA GATGGGCTCT GTGGAAGGGG CCAGCATGGG GCCTCTGGGA 
CCACAGCAAC ACTTGCACAA CCTCCTGGAG GGCGAGGCAT GCAAGCAGCA GGAGGGCCTA 
GCTTTATACC ACGGTGGGCT GCAGCCCCCC CTTCTCTCCC GCAGACAGAG TTTGGAAACA 
CAGTACCTCA CCCACAGACT TCAGGCTAAC ATCCTGGCTA ACAGCCCTGC GAGCTGCCAA 
CTTTTCTGCA AGGAGACGCC GAGAAGTTTA GAGCAGCAGC TACAGGAACA CAGGCTGCAT 
CAGAAGAGGA TGTATCTGCA GCAGCACCAG TCACAGGGCA TGGGACTCCA GGCCTACTTC 
AACCAGATGC AGATAGCTGA GGGCTCCTAC GCCCCAAGCG GCCCCATCCT GGATCCATCA 
GCCGCCCAGA ACTCCCCCCA GGCCCCCTCC ATGTCGGCCC CCCACCAGCC TCAGTCCCAG 
CAGCAGGGCA GTCCCCAAGC CTCACCCCCT TTCAGCCACA CCCACAGCTT GAGCCCTTTG 
ATGGAACCCG GCGAGGGCCT GGTTTACGAC CCGTACATGA GCCACCACCA CCCTTACACC 
CAACAGCTGC CCCCCGGAGC TCACCTCATG CACCACCCAC TCCACCATCA GCATTCTCTG 
GAGGCCCCTG TTGGCGGCTT GGGCTTCAGC TACCCAGCAG GCTGTGATCC ACATTCCCCT 
GGTCCTTCCG CTGAGGCTCT CCTGCCAGAG CAATACGAGT TCTCCATGGA CGCCAGCCTG 
CTGCCTCAGC CTGGTCCAGG GGAGGCAGAC GCTGGAGGCC CCGTGGCAGC GCAGACAGAC 
GGCTTAGGCA TGCCTAAACT GCAGGCGTCC CTCCTGGACA GTGAAATGAT GGAGACCGTG 
GACTCGCAGC ATGGCTTCGT GCTGGTCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sik2 ( XM_004075670.4 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_004075718.1 Oryzias latipes salt inducible kinase 2 (sik2), mRNA.

Price & Availability↑

CloneID	OJa09997
Clone ID Related Accession (Same CDS sequence)	XM_004075670.3 , XM_004075670.4
Accession Version	XM_004075670.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-14
Organism	Oryzias latipes(Japanese medaka)
Product	serine/threonine-protein kinase SIK2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019871.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_004075670.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCATGG CTGACGGTGT CCAAAAGCCG CTGACCCGGG GTCCGGTCCG AGTGGGCTTC 
TACGACATCG AACGCACTTT AGGAAAGGGC AATTTCGCCG TGGTGAAATT GGCTAGACAC 
CGAATAACCA AGACCGAGGT GGCCATTAAG ATCATTGACA AGACCCAACT AGACGCTGTA 
AACCTGGAGA AGATCTACAG GGAAGTTCAA ATCATGAAGA TGTTGGACCA CCCTCACATC 
ATTAAGCTTT ACCAGGTGAT GGAAACAAAG AACATGCTTT ACTTAGTCAC GGAGTATGCC 
AAGAGCGGGG AGATCTTCGA CTATCTGGCA AAGCATGGGC GTCTCAGTGA GCTGGAGGCC 
AGGAGGAAGT TTTGGCAGAT TCTGTCTGCA GTCGAGTACT GTCACAACCG GAACATTGTC 
CACAGAGACC TGAAGGCTGA AAATTTGCTG TTGGACGGTC ACATGAACAT CAAGATTGCA 
GATTTTGGAT TTGGAAACTT CTTTAAACAA GGAGAGCCTT TGGCTACATG GTGTGGCAGC 
CCGCCATACG CTGCTCCAGA GGTCTTTGAA GGACAACAGT ATGAGGGCCC ACAGCTGGAC 
ATCTGGAGTA TGGGGGTGGT GCTTTATGTG CTGGTGTGCG GGGCGTTGCC CTTTGACGGG 
CCCAGTCTGC CTGTCCTCCG ACAGAGAGTC CTAGAAGGAA GGTTCCGCAT CCCATACTTC 
ATGACTGAAG ACTGTGAACA CTTGATCCGC AGGATGTTGG TCCTGGACCC GTCCAAACGC 
CTGTCCTTGG CACAGATTAA GGAGCACAAA TGGATGGCTT TGGACGTCCC AGCACAGAGG 
CCCGTCCTGT ACCAACAGCC ACTGTCAGCT GAGGGTGAGA TGGGTGTAGG AGAATACAGC 
GAACAGGTCC TTCGCCTGAT GCACAGCCTT GGCATCGACC AACAAAAGAC TATAGAGTCT 
CTTCAGAATA AAAGCTACAA CCACTTCGCT GCGATCTACT ACCTGCTGGT CGAGCGGCTG 
AAGGCCCATC GATGCAGCTT CCCTGTGGAA CAAAGGCTCG ACGCCCGCCA GCGGCGCCCC 
AGCACCATCG CTGAGCAGAC GGTCATCAAG TCAACCAGCG GCTCAGCCCA GGTGGGTTTG 
CTTCCACAGG GCGTTCGTCT GTTGCGTTCC CCCGCGGTTC CTCAGCCCTC GTCGGATGCT 
TTTACCTTCC CCCAGACTGC ATGTCCCCTG GAGCAGAACT TCCTTGTGGA GGATGTCAGC 
ACACCCAAAG TGAATGGCTG CCTTCTGGAC CCCCTTCCTC CCTCTACTGT CCTCCGCAAG 
TCCTCCACTT CGTCACCTAG CAACATGATG GAGACCTCGA TTGACGAAGG CATTGAGACT 
GAGGAGCCCG ACACGGAGGA CGACACGCCC CACCTTCTCT CAGCCTATCA GACGACTCGA 
TTTGGCCAGA GGCGGCACAC CTTATCTGAA GTTTCCAACC CGCCTGGGCC TTCAAGCCAA 
GGTAAATATT TCACTTTAGG CCACAACACC TCCATGGGCA GCGTGGACTC GGACATTGGC 
TACGATATGG GCTCCGTGCA CAGCGACCTC AGTCTGCTGG AGGACGCGCC CTCCCTCAGT 
GAGGTGGTAC CATCCAACAG CTCTGCTCCT GGCATAGCTG CACCTTTTCT GAGTGCCAGG 
CCACCCAACC CAGCAATGGC TGCCCTGACT TCTCAGCACA GGGAGGCGCA CAACCGATCC 
CCGATAAGCT TCCGAGAAGG ACGCCGCGCC TCTGACACCT CCCTCACCCA AGGCCTGGTG 
GCGTTTCGGC AGCACCTCCA AAACTTGGCA AGGACCAAAG GCATCCTGGA CCTCAACAAA 
GTCCAGATGC TGGTGGAGCA GATGGGCTCT GTGGAAGGGG CCAGCATGGG GCCTCTGGGA 
CCACAGCAAC ACTTGCACAA CCTCCTGGAG GGCGAGGCAT GCAAGCAGCA GGAGGGCCTA 
GCTTTATACC ACGGTGGGCT GCAGCCCCCC CTTCTCTCCC GCAGACAGAG TTTGGAAACA 
CAGTACCTCA CCCACAGACT TCAGGCTAAC ATCCTGGCTA ACAGCCCTGC GAGCTGCCAA 
CTTTTCTGCA AGGAGACGCC GAGAAGTTTA GAGCAGCAGC TACAGGAACA CAGGCTGCAT 
CAGAAGAGGA TGTATCTGCA GCAGCACCAG TCACAGGGCA TGGGACTCCA GGCCTACTTC 
AACCAGATGC AGATAGCTGA GGGCTCCTAC GCCCCAAGCG GCCCCATCCT GGATCCATCA 
GCCGCCCAGA ACTCCCCCCA GGCCCCCTCC ATGTCGGCCC CCCACCAGCC TCAGTCCCAG 
CAGCAGGGCA GTCCCCAAGC CTCACCCCCT TTCAGCCACA CCCACAGCTT GAGCCCTTTG 
ATGGAACCCG GCGAGGGCCT GGTTTACGAC CCGTACATGA GCCACCACCA CCCTTACACC 
CAACAGCTGC CCCCCGGAGC TCACCTCATG CACCACCCAC TCCACCATCA GCATTCTCTG 
GAGGCCCCTG TTGGCGGCTT GGGCTTCAGC TACCCAGCAG GCTGTGATCC ACATTCCCCT 
GGTCCTTCCG CTGAGGCTCT CCTGCCAGAG CAATACGAGT TCTCCATGGA CGCCAGCCTG 
CTGCCTCAGC CTGGTCCAGG GGAGGCAGAC GCTGGAGGCC CCGTGGCAGC GCAGACAGAC 
GGCTTAGGCA TGCCTAAACT GCAGGCGTCC CTCCTGGACA GTGAAATGAT GGAGACCGTG 
GACTCGCAGC ATGGCTTCGT GCTGGTCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004075718.1
CDS	383..3175
Translation	MVMADGVQKPLTRGPVRVGFYDIERTLGKGNFAVVKLARHRITKTEVAIKIIDKTQLDAVNLEKIYREVQIMKMLDHPHIIKLYQVMETKNMLYLVTEYAKSGEIFDYLAKHGRLSELEARRKFWQILSAVEYCHNRNIVHRDLKAENLLLDGHMNIKIADFGFGNFFKQGEPLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPSLPVLRQRVLEGRFRIPYFMTEDCEHLIRRMLVLDPSKRLSLAQIKEHKWMALDVPAQRPVLYQQPLSAEGEMGVGEYSEQVLRLMHSLGIDQQKTIESLQNKSYNHFAAIYYLLVERLKAHRCSFPVEQRLDARQRRPSTIAEQTVIKSTSGSAQVGLLPQGVRLLRSPAVPQPSSDAFTFPQTACPLEQNFLVEDVSTPKVNGCLLDPLPPSTVLRKSSTSSPSNMMETSIDEGIETEEPDTEDDTPHLLSAYQTTRFGQRRHTLSEVSNPPGPSSQGKYFTLGHNTSMGSVDSDIGYDMGSVHSDLSLLEDAPSLSEVVPSNSSAPGIAAPFLSARPPNPAMAALTSQHREAHNRSPISFREGRRASDTSLTQGLVAFRQHLQNLARTKGILDLNKVQMLVEQMGSVEGASMGPLGPQQHLHNLLEGEACKQQEGLALYHGGLQPPLLSRRQSLETQYLTHRLQANILANSPASCQLFCKETPRSLEQQLQEHRLHQKRMYLQQHQSQGMGLQAYFNQMQIAEGSYAPSGPILDPSAAQNSPQAPSMSAPHQPQSQQQGSPQASPPFSHTHSLSPLMEPGEGLVYDPYMSHHHPYTQQLPPGAHLMHHPLHHQHSLEAPVGGLGFSYPAGCDPHSPGPSAEALLPEQYEFSMDASLLPQPGPGEADAGGPVAAQTDGLGMPKLQASLLDSEMMETVDSQHGFVLVN

Target ORF information:

RefSeq Version	XM_004075670.4
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes salt inducible kinase 2 (sik2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004075670.4

ORF Insert Sequence:

ATGGTCATGG CTGACGGTGT CCAAAAGCCG CTGACCCGGG GTCCGGTCCG AGTGGGCTTC 
TACGACATCG AACGCACTTT AGGAAAGGGC AATTTCGCCG TGGTGAAATT GGCTAGACAC 
CGAATAACCA AGACCGAGGT GGCCATTAAG ATCATTGACA AGACCCAACT AGACGCTGTA 
AACCTGGAGA AGATCTACAG GGAAGTTCAA ATCATGAAGA TGTTGGACCA CCCTCACATC 
ATTAAGCTTT ACCAGGTGAT GGAAACAAAG AACATGCTTT ACTTAGTCAC GGAGTATGCC 
AAGAGCGGGG AGATCTTCGA CTATCTGGCA AAGCATGGGC GTCTCAGTGA GCTGGAGGCC 
AGGAGGAAGT TTTGGCAGAT TCTGTCTGCA GTCGAGTACT GTCACAACCG GAACATTGTC 
CACAGAGACC TGAAGGCTGA AAATTTGCTG TTGGACGGTC ACATGAACAT CAAGATTGCA 
GATTTTGGAT TTGGAAACTT CTTTAAACAA GGAGAGCCTT TGGCTACATG GTGTGGCAGC 
CCGCCATACG CTGCTCCAGA GGTCTTTGAA GGACAACAGT ATGAGGGCCC ACAGCTGGAC 
ATCTGGAGTA TGGGGGTGGT GCTTTATGTG CTGGTGTGCG GGGCGTTGCC CTTTGACGGG 
CCCAGTCTGC CTGTCCTCCG ACAGAGAGTC CTAGAAGGAA GGTTCCGCAT CCCATACTTC 
ATGACTGAAG ACTGTGAACA CTTGATCCGC AGGATGTTGG TCCTGGACCC GTCCAAACGC 
CTGTCCTTGG CACAGATTAA GGAGCACAAA TGGATGGCTT TGGACGTCCC AGCACAGAGG 
CCCGTCCTGT ACCAACAGCC ACTGTCAGCT GAGGGTGAGA TGGGTGTAGG AGAATACAGC 
GAACAGGTCC TTCGCCTGAT GCACAGCCTT GGCATCGACC AACAAAAGAC TATAGAGTCT 
CTTCAGAATA AAAGCTACAA CCACTTCGCT GCGATCTACT ACCTGCTGGT CGAGCGGCTG 
AAGGCCCATC GATGCAGCTT CCCTGTGGAA CAAAGGCTCG ACGCCCGCCA GCGGCGCCCC 
AGCACCATCG CTGAGCAGAC GGTCATCAAG TCAACCAGCG GCTCAGCCCA GGTGGGTTTG 
CTTCCACAGG GCGTTCGTCT GTTGCGTTCC CCCGCGGTTC CTCAGCCCTC GTCGGATGCT 
TTTACCTTCC CCCAGACTGC ATGTCCCCTG GAGCAGAACT TCCTTGTGGA GGATGTCAGC 
ACACCCAAAG TGAATGGCTG CCTTCTGGAC CCCCTTCCTC CCTCTACTGT CCTCCGCAAG 
TCCTCCACTT CGTCACCTAG CAACATGATG GAGACCTCGA TTGACGAAGG CATTGAGACT 
GAGGAGCCCG ACACGGAGGA CGACACGCCC CACCTTCTCT CAGCCTATCA GACGACTCGA 
TTTGGCCAGA GGCGGCACAC CTTATCTGAA GTTTCCAACC CGCCTGGGCC TTCAAGCCAA 
GGTAAATATT TCACTTTAGG CCACAACACC TCCATGGGCA GCGTGGACTC GGACATTGGC 
TACGATATGG GCTCCGTGCA CAGCGACCTC AGTCTGCTGG AGGACGCGCC CTCCCTCAGT 
GAGGTGGTAC CATCCAACAG CTCTGCTCCT GGCATAGCTG CACCTTTTCT GAGTGCCAGG 
CCACCCAACC CAGCAATGGC TGCCCTGACT TCTCAGCACA GGGAGGCGCA CAACCGATCC 
CCGATAAGCT TCCGAGAAGG ACGCCGCGCC TCTGACACCT CCCTCACCCA AGGCCTGGTG 
GCGTTTCGGC AGCACCTCCA AAACTTGGCA AGGACCAAAG GCATCCTGGA CCTCAACAAA 
GTCCAGATGC TGGTGGAGCA GATGGGCTCT GTGGAAGGGG CCAGCATGGG GCCTCTGGGA 
CCACAGCAAC ACTTGCACAA CCTCCTGGAG GGCGAGGCAT GCAAGCAGCA GGAGGGCCTA 
GCTTTATACC ACGGTGGGCT GCAGCCCCCC CTTCTCTCCC GCAGACAGAG TTTGGAAACA 
CAGTACCTCA CCCACAGACT TCAGGCTAAC ATCCTGGCTA ACAGCCCTGC GAGCTGCCAA 
CTTTTCTGCA AGGAGACGCC GAGAAGTTTA GAGCAGCAGC TACAGGAACA CAGGCTGCAT 
CAGAAGAGGA TGTATCTGCA GCAGCACCAG TCACAGGGCA TGGGACTCCA GGCCTACTTC 
AACCAGATGC AGATAGCTGA GGGCTCCTAC GCCCCAAGCG GCCCCATCCT GGATCCATCA 
GCCGCCCAGA ACTCCCCCCA GGCCCCCTCC ATGTCGGCCC CCCACCAGCC TCAGTCCCAG 
CAGCAGGGCA GTCCCCAAGC CTCACCCCCT TTCAGCCACA CCCACAGCTT GAGCCCTTTG 
ATGGAACCCG GCGAGGGCCT GGTTTACGAC CCGTACATGA GCCACCACCA CCCTTACACC 
CAACAGCTGC CCCCCGGAGC TCACCTCATG CACCACCCAC TCCACCATCA GCATTCTCTG 
GAGGCCCCTG TTGGCGGCTT GGGCTTCAGC TACCCAGCAG GCTGTGATCC ACATTCCCCT 
GGTCCTTCCG CTGAGGCTCT CCTGCCAGAG CAATACGAGT TCTCCATGGA CGCCAGCCTG 
CTGCCTCAGC CTGGTCCAGG GGAGGCAGAC GCTGGAGGCC CCGTGGCAGC GCAGACAGAC 
GGCTTAGGCA TGCCTAAACT GCAGGCGTCC CTCCTGGACA GTGAAATGAT GGAGACCGTG 
GACTCGCAGC ATGGCTTCGT GCTGGTCAAC TAA