nek9 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	nek9
Entrez Gene ID	101166349
Full Name	NIMA related kinase 9
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011490412.2	XP_011488714.1	serine/threonine-protein kinase Nek9
XM_011490412.3	XP_011488714.1	serine/threonine-protein kinase Nek9

Pathways from BioSystems
Gene Ontology

The following nek9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nek9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa30093	XM_011490412.3 Latest version!	Oryzias latipes NIMA related kinase 9 (nek9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OJa30093	XM_011490412.2	Oryzias latipes NIMA related kinase 9 (nek9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nek9 ( XM_011490412.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488714.1 Oryzias latipes NIMA related kinase 9 (nek9), mRNA.

Price & Availability↑

CloneID	OJa30093
Clone ID Related Accession (Same CDS sequence)	XM_011490412.2 , XM_011490412.3
Accession Version	XM_011490412.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-06
Organism	Oryzias latipes(Japanese medaka)
Product	serine/threonine-protein kinase Nek9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011490412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTGC AGGAATATGA GCGGCATTTC GAGTCGCTAA ATTCGGATTT AGGCGGCGAG 
TCTGTGGGCA GTGAGCGGTC ATCTTCTGGG ACGTTTTATG GCGAAGAGGA GAAGTTACAC 
TACATTCCTA TCCGGATCCT GGGGAGGGGG GCGTTTGGGG AAGCGACTCT GTACAGAAGA 
ACTGAGGATA ATTCCTTGGT GGTGTGGAAA GAGGTGGACT TGAACTCGCT CTCAGAGAAG 
GAACGTCGAG ATGTCATGAA TGAAATCAAC ATCCTCTCCG TGTTGGAGCA CAACAACATC 
ATAGCTTACT TCAACCACTT TATGGACAAA AACTCTCTGC TCATTGAGTT GGAGTATTGC 
AACGGAGGAA ATTTGTACGA TAAAATCGTC CAGCAGAAAG GAGTTCCCTT TTCCGAGAAG 
GTAGTGGTAT GGTACCTCTA TCAAATTGCT TCAGCAGTGG CCTACATTCA CAAAGCTGGG 
ATCTTACACA GAGATATCAA AACTTTGAAC ATTTTCCTGA CCAAGACAGA CCTCATCAAG 
CTGGGGGATT ATGGCCTTGC AAAGAAGCTG GACTCTGAGT TTTCAATGGC AGAGACATGT 
GTAGGAACTC CATATTACAT GTCACCTGAA TTGTGCCAGG GAGCAAAGTA CAACTTCAAA 
TCCGACATTT GGGCTATGGG TTGTGTCCTT TATGAAGTCT TAACTCTAAA AAGAACATTT 
GATGCAACAA ACCCGTTAAA CCTCTGTGTT AAAATAGTCC AAGGCAACTG GACTATGGAA 
GGGGTCTCTG ACATTTATTC GTCCGGACTG TTGAAGCTGG TGTACGAGTG TCTGCATCAG 
GATCCTGCAA AAAGGCCAAC AGCTGAGCAC ATTCTGGACC AGCCTTCCAT CTCCTGCTGC 
AGACAGGAGC TTGAGGAGCA AGTTGTCTTG CTGAATTCAT CAATGAAGAG ACCAAAATTA 
AATCCAGTGT CGGAGAGTTC CATTGCCGTG GTGACACGTT CAAGGGAGGT GTACTATTGG 
GGAGGAGGGA AGTTTACCCC CCAAAAACTT GACTCATTCA AAGCAGGCAG CGGGGCCCAG 
CATGTGTGTG CAGGAGAGAA TCATTTTGCA GTGGTGACGG TGGAGAAGGA GCTGTACACC 
TGGGCTGATG TCCAAGGTGG GGCCAAAATG GTGGGGCAGC TGGGACATGG GGATCAGGCC 
TCCTATCGAC AGCCGAAGCG GGTGGAGAAG CTCCAGGGGA AGGCCATCCG GCAGGTGGCG 
TGTGGAGCGG ACTTTACTGC CTGTGTGACT GATGAAGACC AGATGTACAT GTTCGGGTCA 
GACTATGACG GTTGCATCGG GGTTGAGGGT CAGCTGGGGT CGCAGGTTTT GGAGCCAGTT 
CTACTGGAGT TTTTTGAGGG GCGACCTGTT CAGCTGGTTT CGTGTGGAGA CAACCATGTG 
GTGGTGTTGA CCCGGGATAA GGACATCTAC TCATGGGGCT GCGGAGAATA CGGGCGTCTT 
GGTCTGGAGA GTGAGGACGA ATTTTCCTCT CCGATGCAAG TAAAGGTTCC TATAGGTGCC 
AGTATTTCCT CCGTCTCATG TGGCAGCGAT AGCACCTTCT TCTTGACAGA GTCTGGGAAA 
GTGCTAGCGT GCGGAAACAA CGAGTTCAAC AAACTGGGGT TAAATCAGGG CATCTCTGGA 
ATCAAGAATC ATCCCGGAGA GGGTTACCAA GGGATACCGT ACACCACCGC AATGACCCTG 
GTGAAACAGC TGTCACGCTT CAAGATCCAG TCCATTTCTC CAGGGAAGAC TCACACTGCT 
GCTATTGATG AACGAGGTCG TCTGATCACG TTTGGTTGCA ACAAATTTGG ACAGCTGGGT 
GTGAAAGACT TCAAGAAGCA CGCTGGTGTC CAAGTCCTTG TCGGTCCATT TGGAGGCAAG 
ACGGTGACCA AAGTGGCTTG TGGAGATGGA TTCACCATCG CTGCTACAGA AGACAATCAG 
ATCTTCGCAT GGGGAAATGC AGGAAATGGG AGGCTAGGAA TGCCTCCTGA TAAGGGCTTT 
GGTTCAGAGG TTTGCCCCGC CCTGCCCAGG CCCATTTTTG GTTCCCTCCA TCATGTACCC 
GACCTCTCGT GCCGCGGCTG GCACACTATT ATTATCATGG AAAAAGTCCT CAACTCCAAG 
ACCATCCGCT CAAACAGCAG TGGACTCTCA ATTGGCTTCG GCCATGACGG ATCGACATCA 
ACCGTTGATC TGGACTTGGA TCCCGGATCG GAGACCGAAT GTCGTGGCAG GAGTCTTGGA 
GGCACGCGAG AGGTTAACGT TGACGGATGC TCGATGGAGG CCCCAGTGAT GTCGGCTGCA 
AACCAGACTG GAGACAGCTC CTGTCCGCTG TGGCTTCAAA AGGAGCTTGA AGATGCAGAG 
TTCATCCCAA TGCCAGATAC GTGCGACAAC GACCAGCTGC CTTCTGTCTC CGAGAGCTTT 
ACTCGGCCAT ATGAGGATCT GAAAGAGCTC AAGGCAGCAG CAGATGCTGT CAGCACCGCA 
AAAGGCCTTT CGGCCACACG GATGAGCTTT GATACAGTCA ACGGTCTGGA TGGAGGGGAT 
GTCTGCAGAA AGGGGGAGTC TGGTGCTTGC TGTAAAGCAA GTAGAGAGGT GGCTCAGTTG 
CAAGAAATGC TTGCCCATCA AGAGAGGAGA ATTCAGATGC TGGAAAAGCA GGTCAGTGAA 
CAGAAAAAGG AGAATGAGAA GCTTTGGGCA GCCATCAACC GCACCGCTGT GCAGGAAAGG 
GGTCTTAACA GCGACAGAAA CTGCCTCTCT GCTCCTGGGG GAGGCGGAAG AAGAGGGGAA 
CTCACGAATC ACGGAGGCCG ATCTGCCGGG GCAAGCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488714.1
CDS	273..3134
Translation	MSVQEYERHFESLNSDLGGESVGSERSSSGTFYGEEEKLHYIPIRILGRGAFGEATLYRRTEDNSLVVWKEVDLNSLSEKERRDVMNEINILSVLEHNNIIAYFNHFMDKNSLLIELEYCNGGNLYDKIVQQKGVPFSEKVVVWYLYQIASAVAYIHKAGILHRDIKTLNIFLTKTDLIKLGDYGLAKKLDSEFSMAETCVGTPYYMSPELCQGAKYNFKSDIWAMGCVLYEVLTLKRTFDATNPLNLCVKIVQGNWTMEGVSDIYSSGLLKLVYECLHQDPAKRPTAEHILDQPSISCCRQELEEQVVLLNSSMKRPKLNPVSESSIAVVTRSREVYYWGGGKFTPQKLDSFKAGSGAQHVCAGENHFAVVTVEKELYTWADVQGGAKMVGQLGHGDQASYRQPKRVEKLQGKAIRQVACGADFTACVTDEDQMYMFGSDYDGCIGVEGQLGSQVLEPVLLEFFEGRPVQLVSCGDNHVVVLTRDKDIYSWGCGEYGRLGLESEDEFSSPMQVKVPIGASISSVSCGSDSTFFLTESGKVLACGNNEFNKLGLNQGISGIKNHPGEGYQGIPYTTAMTLVKQLSRFKIQSISPGKTHTAAIDERGRLITFGCNKFGQLGVKDFKKHAGVQVLVGPFGGKTVTKVACGDGFTIAATEDNQIFAWGNAGNGRLGMPPDKGFGSEVCPALPRPIFGSLHHVPDLSCRGWHTIIIMEKVLNSKTIRSNSSGLSIGFGHDGSTSTVDLDLDPGSETECRGRSLGGTREVNVDGCSMEAPVMSAANQTGDSSCPLWLQKELEDAEFIPMPDTCDNDQLPSVSESFTRPYEDLKELKAAADAVSTAKGLSATRMSFDTVNGLDGGDVCRKGESGACCKASREVAQLQEMLAHQERRIQMLEKQVSEQKKENEKLWAAINRTAVQERGLNSDRNCLSAPGGGGRRGELTNHGGRSAGASV

Target ORF information:

RefSeq Version	XM_011490412.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NIMA related kinase 9 (nek9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490412.2

ORF Insert Sequence:

ATGTCAGTGC AGGAATATGA GCGGCATTTC GAGTCGCTAA ATTCGGATTT AGGCGGCGAG 
TCTGTGGGCA GTGAGCGGTC ATCTTCTGGG ACGTTTTATG GCGAAGAGGA GAAGTTACAC 
TACATTCCTA TCCGGATCCT GGGGAGGGGG GCGTTTGGGG AAGCGACTCT GTACAGAAGA 
ACTGAGGATA ATTCCTTGGT GGTGTGGAAA GAGGTGGACT TGAACTCGCT CTCAGAGAAG 
GAACGTCGAG ATGTCATGAA TGAAATCAAC ATCCTCTCCG TGTTGGAGCA CAACAACATC 
ATAGCTTACT TCAACCACTT TATGGACAAA AACTCTCTGC TCATTGAGTT GGAGTATTGC 
AACGGAGGAA ATTTGTACGA TAAAATCGTC CAGCAGAAAG GAGTTCCCTT TTCCGAGAAG 
GTAGTGGTAT GGTACCTCTA TCAAATTGCT TCAGCAGTGG CCTACATTCA CAAAGCTGGG 
ATCTTACACA GAGATATCAA AACTTTGAAC ATTTTCCTGA CCAAGACAGA CCTCATCAAG 
CTGGGGGATT ATGGCCTTGC AAAGAAGCTG GACTCTGAGT TTTCAATGGC AGAGACATGT 
GTAGGAACTC CATATTACAT GTCACCTGAA TTGTGCCAGG GAGCAAAGTA CAACTTCAAA 
TCCGACATTT GGGCTATGGG TTGTGTCCTT TATGAAGTCT TAACTCTAAA AAGAACATTT 
GATGCAACAA ACCCGTTAAA CCTCTGTGTT AAAATAGTCC AAGGCAACTG GACTATGGAA 
GGGGTCTCTG ACATTTATTC GTCCGGACTG TTGAAGCTGG TGTACGAGTG TCTGCATCAG 
GATCCTGCAA AAAGGCCAAC AGCTGAGCAC ATTCTGGACC AGCCTTCCAT CTCCTGCTGC 
AGACAGGAGC TTGAGGAGCA AGTTGTCTTG CTGAATTCAT CAATGAAGAG ACCAAAATTA 
AATCCAGTGT CGGAGAGTTC CATTGCCGTG GTGACACGTT CAAGGGAGGT GTACTATTGG 
GGAGGAGGGA AGTTTACCCC CCAAAAACTT GACTCATTCA AAGCAGGCAG CGGGGCCCAG 
CATGTGTGTG CAGGAGAGAA TCATTTTGCA GTGGTGACGG TGGAGAAGGA GCTGTACACC 
TGGGCTGATG TCCAAGGTGG GGCCAAAATG GTGGGGCAGC TGGGACATGG GGATCAGGCC 
TCCTATCGAC AGCCGAAGCG GGTGGAGAAG CTCCAGGGGA AGGCCATCCG GCAGGTGGCG 
TGTGGAGCGG ACTTTACTGC CTGTGTGACT GATGAAGACC AGATGTACAT GTTCGGGTCA 
GACTATGACG GTTGCATCGG GGTTGAGGGT CAGCTGGGGT CGCAGGTTTT GGAGCCAGTT 
CTACTGGAGT TTTTTGAGGG GCGACCTGTT CAGCTGGTTT CGTGTGGAGA CAACCATGTG 
GTGGTGTTGA CCCGGGATAA GGACATCTAC TCATGGGGCT GCGGAGAATA CGGGCGTCTT 
GGTCTGGAGA GTGAGGACGA ATTTTCCTCT CCGATGCAAG TAAAGGTTCC TATAGGTGCC 
AGTATTTCCT CCGTCTCATG TGGCAGCGAT AGCACCTTCT TCTTGACAGA GTCTGGGAAA 
GTGCTAGCGT GCGGAAACAA CGAGTTCAAC AAACTGGGGT TAAATCAGGG CATCTCTGGA 
ATCAAGAATC ATCCCGGAGA GGGTTACCAA GGGATACCGT ACACCACCGC AATGACCCTG 
GTGAAACAGC TGTCACGCTT CAAGATCCAG TCCATTTCTC CAGGGAAGAC TCACACTGCT 
GCTATTGATG AACGAGGTCG TCTGATCACG TTTGGTTGCA ACAAATTTGG ACAGCTGGGT 
GTGAAAGACT TCAAGAAGCA CGCTGGTGTC CAAGTCCTTG TCGGTCCATT TGGAGGCAAG 
ACGGTGACCA AAGTGGCTTG TGGAGATGGA TTCACCATCG CTGCTACAGA AGACAATCAG 
ATCTTCGCAT GGGGAAATGC AGGAAATGGG AGGCTAGGAA TGCCTCCTGA TAAGGGCTTT 
GGTTCAGAGG TTTGCCCCGC CCTGCCCAGG CCCATTTTTG GTTCCCTCCA TCATGTACCC 
GACCTCTCGT GCCGCGGCTG GCACACTATT ATTATCATGG AAAAAGTCCT CAACTCCAAG 
ACCATCCGCT CAAACAGCAG TGGACTCTCA ATTGGCTTCG GCCATGACGG ATCGACATCA 
ACCGTTGATC TGGACTTGGA TCCCGGATCG GAGACCGAAT GTCGTGGCAG GAGTCTTGGA 
GGCACGCGAG AGGTTAACGT TGACGGATGC TCGATGGAGG CCCCAGTGAT GTCGGCTGCA 
AACCAGACTG GAGACAGCTC CTGTCCGCTG TGGCTTCAAA AGGAGCTTGA AGATGCAGAG 
TTCATCCCAA TGCCAGATAC GTGCGACAAC GACCAGCTGC CTTCTGTCTC CGAGAGCTTT 
ACTCGGCCAT ATGAGGATCT GAAAGAGCTC AAGGCAGCAG CAGATGCTGT CAGCACCGCA 
AAAGGCCTTT CGGCCACACG GATGAGCTTT GATACAGTCA ACGGTCTGGA TGGAGGGGAT 
GTCTGCAGAA AGGGGGAGTC TGGTGCTTGC TGTAAAGCAA GTAGAGAGGT GGCTCAGTTG 
CAAGAAATGC TTGCCCATCA AGAGAGGAGA ATTCAGATGC TGGAAAAGCA GGTCAGTGAA 
CAGAAAAAGG AGAATGAGAA GCTTTGGGCA GCCATCAACC GCACCGCTGT GCAGGAAAGG 
GGTCTTAACA GCGACAGAAA CTGCCTCTCT GCTCCTGGGG GAGGCGGAAG AAGAGGGGAA 
CTCACGAATC ACGGAGGCCG ATCTGCCGGG GCAAGCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nek9 ( XM_011490412.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488714.1 Oryzias latipes NIMA related kinase 9 (nek9), mRNA.

Price & Availability↑

CloneID	OJa30093
Clone ID Related Accession (Same CDS sequence)	XM_011490412.2 , XM_011490412.3
Accession Version	XM_011490412.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-14
Organism	Oryzias latipes(Japanese medaka)
Product	serine/threonine-protein kinase Nek9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011490412.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTGC AGGAATATGA GCGGCATTTC GAGTCGCTAA ATTCGGATTT AGGCGGCGAG 
TCTGTGGGCA GTGAGCGGTC ATCTTCTGGG ACGTTTTATG GCGAAGAGGA GAAGTTACAC 
TACATTCCTA TCCGGATCCT GGGGAGGGGG GCGTTTGGGG AAGCGACTCT GTACAGAAGA 
ACTGAGGATA ATTCCTTGGT GGTGTGGAAA GAGGTGGACT TGAACTCGCT CTCAGAGAAG 
GAACGTCGAG ATGTCATGAA TGAAATCAAC ATCCTCTCCG TGTTGGAGCA CAACAACATC 
ATAGCTTACT TCAACCACTT TATGGACAAA AACTCTCTGC TCATTGAGTT GGAGTATTGC 
AACGGAGGAA ATTTGTACGA TAAAATCGTC CAGCAGAAAG GAGTTCCCTT TTCCGAGAAG 
GTAGTGGTAT GGTACCTCTA TCAAATTGCT TCAGCAGTGG CCTACATTCA CAAAGCTGGG 
ATCTTACACA GAGATATCAA AACTTTGAAC ATTTTCCTGA CCAAGACAGA CCTCATCAAG 
CTGGGGGATT ATGGCCTTGC AAAGAAGCTG GACTCTGAGT TTTCAATGGC AGAGACATGT 
GTAGGAACTC CATATTACAT GTCACCTGAA TTGTGCCAGG GAGCAAAGTA CAACTTCAAA 
TCCGACATTT GGGCTATGGG TTGTGTCCTT TATGAAGTCT TAACTCTAAA AAGAACATTT 
GATGCAACAA ACCCGTTAAA CCTCTGTGTT AAAATAGTCC AAGGCAACTG GACTATGGAA 
GGGGTCTCTG ACATTTATTC GTCCGGACTG TTGAAGCTGG TGTACGAGTG TCTGCATCAG 
GATCCTGCAA AAAGGCCAAC AGCTGAGCAC ATTCTGGACC AGCCTTCCAT CTCCTGCTGC 
AGACAGGAGC TTGAGGAGCA AGTTGTCTTG CTGAATTCAT CAATGAAGAG ACCAAAATTA 
AATCCAGTGT CGGAGAGTTC CATTGCCGTG GTGACACGTT CAAGGGAGGT GTACTATTGG 
GGAGGAGGGA AGTTTACCCC CCAAAAACTT GACTCATTCA AAGCAGGCAG CGGGGCCCAG 
CATGTGTGTG CAGGAGAGAA TCATTTTGCA GTGGTGACGG TGGAGAAGGA GCTGTACACC 
TGGGCTGATG TCCAAGGTGG GGCCAAAATG GTGGGGCAGC TGGGACATGG GGATCAGGCC 
TCCTATCGAC AGCCGAAGCG GGTGGAGAAG CTCCAGGGGA AGGCCATCCG GCAGGTGGCG 
TGTGGAGCGG ACTTTACTGC CTGTGTGACT GATGAAGACC AGATGTACAT GTTCGGGTCA 
GACTATGACG GTTGCATCGG GGTTGAGGGT CAGCTGGGGT CGCAGGTTTT GGAGCCAGTT 
CTACTGGAGT TTTTTGAGGG GCGACCTGTT CAGCTGGTTT CGTGTGGAGA CAACCATGTG 
GTGGTGTTGA CCCGGGATAA GGACATCTAC TCATGGGGCT GCGGAGAATA CGGGCGTCTT 
GGTCTGGAGA GTGAGGACGA ATTTTCCTCT CCGATGCAAG TAAAGGTTCC TATAGGTGCC 
AGTATTTCCT CCGTCTCATG TGGCAGCGAT AGCACCTTCT TCTTGACAGA GTCTGGGAAA 
GTGCTAGCGT GCGGAAACAA CGAGTTCAAC AAACTGGGGT TAAATCAGGG CATCTCTGGA 
ATCAAGAATC ATCCCGGAGA GGGTTACCAA GGGATACCGT ACACCACCGC AATGACCCTG 
GTGAAACAGC TGTCACGCTT CAAGATCCAG TCCATTTCTC CAGGGAAGAC TCACACTGCT 
GCTATTGATG AACGAGGTCG TCTGATCACG TTTGGTTGCA ACAAATTTGG ACAGCTGGGT 
GTGAAAGACT TCAAGAAGCA CGCTGGTGTC CAAGTCCTTG TCGGTCCATT TGGAGGCAAG 
ACGGTGACCA AAGTGGCTTG TGGAGATGGA TTCACCATCG CTGCTACAGA AGACAATCAG 
ATCTTCGCAT GGGGAAATGC AGGAAATGGG AGGCTAGGAA TGCCTCCTGA TAAGGGCTTT 
GGTTCAGAGG TTTGCCCCGC CCTGCCCAGG CCCATTTTTG GTTCCCTCCA TCATGTACCC 
GACCTCTCGT GCCGCGGCTG GCACACTATT ATTATCATGG AAAAAGTCCT CAACTCCAAG 
ACCATCCGCT CAAACAGCAG TGGACTCTCA ATTGGCTTCG GCCATGACGG ATCGACATCA 
ACCGTTGATC TGGACTTGGA TCCCGGATCG GAGACCGAAT GTCGTGGCAG GAGTCTTGGA 
GGCACGCGAG AGGTTAACGT TGACGGATGC TCGATGGAGG CCCCAGTGAT GTCGGCTGCA 
AACCAGACTG GAGACAGCTC CTGTCCGCTG TGGCTTCAAA AGGAGCTTGA AGATGCAGAG 
TTCATCCCAA TGCCAGATAC GTGCGACAAC GACCAGCTGC CTTCTGTCTC CGAGAGCTTT 
ACTCGGCCAT ATGAGGATCT GAAAGAGCTC AAGGCAGCAG CAGATGCTGT CAGCACCGCA 
AAAGGCCTTT CGGCCACACG GATGAGCTTT GATACAGTCA ACGGTCTGGA TGGAGGGGAT 
GTCTGCAGAA AGGGGGAGTC TGGTGCTTGC TGTAAAGCAA GTAGAGAGGT GGCTCAGTTG 
CAAGAAATGC TTGCCCATCA AGAGAGGAGA ATTCAGATGC TGGAAAAGCA GGTCAGTGAA 
CAGAAAAAGG AGAATGAGAA GCTTTGGGCA GCCATCAACC GCACCGCTGT GCAGGAAAGG 
GGTCTTAACA GCGACAGAAA CTGCCTCTCT GCTCCTGGGG GAGGCGGAAG AAGAGGGGAA 
CTCACGAATC ACGGAGGCCG ATCTGCCGGG GCAAGCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488714.1
CDS	282..3143
Translation	MSVQEYERHFESLNSDLGGESVGSERSSSGTFYGEEEKLHYIPIRILGRGAFGEATLYRRTEDNSLVVWKEVDLNSLSEKERRDVMNEINILSVLEHNNIIAYFNHFMDKNSLLIELEYCNGGNLYDKIVQQKGVPFSEKVVVWYLYQIASAVAYIHKAGILHRDIKTLNIFLTKTDLIKLGDYGLAKKLDSEFSMAETCVGTPYYMSPELCQGAKYNFKSDIWAMGCVLYEVLTLKRTFDATNPLNLCVKIVQGNWTMEGVSDIYSSGLLKLVYECLHQDPAKRPTAEHILDQPSISCCRQELEEQVVLLNSSMKRPKLNPVSESSIAVVTRSREVYYWGGGKFTPQKLDSFKAGSGAQHVCAGENHFAVVTVEKELYTWADVQGGAKMVGQLGHGDQASYRQPKRVEKLQGKAIRQVACGADFTACVTDEDQMYMFGSDYDGCIGVEGQLGSQVLEPVLLEFFEGRPVQLVSCGDNHVVVLTRDKDIYSWGCGEYGRLGLESEDEFSSPMQVKVPIGASISSVSCGSDSTFFLTESGKVLACGNNEFNKLGLNQGISGIKNHPGEGYQGIPYTTAMTLVKQLSRFKIQSISPGKTHTAAIDERGRLITFGCNKFGQLGVKDFKKHAGVQVLVGPFGGKTVTKVACGDGFTIAATEDNQIFAWGNAGNGRLGMPPDKGFGSEVCPALPRPIFGSLHHVPDLSCRGWHTIIIMEKVLNSKTIRSNSSGLSIGFGHDGSTSTVDLDLDPGSETECRGRSLGGTREVNVDGCSMEAPVMSAANQTGDSSCPLWLQKELEDAEFIPMPDTCDNDQLPSVSESFTRPYEDLKELKAAADAVSTAKGLSATRMSFDTVNGLDGGDVCRKGESGACCKASREVAQLQEMLAHQERRIQMLEKQVSEQKKENEKLWAAINRTAVQERGLNSDRNCLSAPGGGGRRGELTNHGGRSAGASV

Target ORF information:

RefSeq Version	XM_011490412.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes NIMA related kinase 9 (nek9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490412.3

ORF Insert Sequence:

ATGTCAGTGC AGGAATATGA GCGGCATTTC GAGTCGCTAA ATTCGGATTT AGGCGGCGAG 
TCTGTGGGCA GTGAGCGGTC ATCTTCTGGG ACGTTTTATG GCGAAGAGGA GAAGTTACAC 
TACATTCCTA TCCGGATCCT GGGGAGGGGG GCGTTTGGGG AAGCGACTCT GTACAGAAGA 
ACTGAGGATA ATTCCTTGGT GGTGTGGAAA GAGGTGGACT TGAACTCGCT CTCAGAGAAG 
GAACGTCGAG ATGTCATGAA TGAAATCAAC ATCCTCTCCG TGTTGGAGCA CAACAACATC 
ATAGCTTACT TCAACCACTT TATGGACAAA AACTCTCTGC TCATTGAGTT GGAGTATTGC 
AACGGAGGAA ATTTGTACGA TAAAATCGTC CAGCAGAAAG GAGTTCCCTT TTCCGAGAAG 
GTAGTGGTAT GGTACCTCTA TCAAATTGCT TCAGCAGTGG CCTACATTCA CAAAGCTGGG 
ATCTTACACA GAGATATCAA AACTTTGAAC ATTTTCCTGA CCAAGACAGA CCTCATCAAG 
CTGGGGGATT ATGGCCTTGC AAAGAAGCTG GACTCTGAGT TTTCAATGGC AGAGACATGT 
GTAGGAACTC CATATTACAT GTCACCTGAA TTGTGCCAGG GAGCAAAGTA CAACTTCAAA 
TCCGACATTT GGGCTATGGG TTGTGTCCTT TATGAAGTCT TAACTCTAAA AAGAACATTT 
GATGCAACAA ACCCGTTAAA CCTCTGTGTT AAAATAGTCC AAGGCAACTG GACTATGGAA 
GGGGTCTCTG ACATTTATTC GTCCGGACTG TTGAAGCTGG TGTACGAGTG TCTGCATCAG 
GATCCTGCAA AAAGGCCAAC AGCTGAGCAC ATTCTGGACC AGCCTTCCAT CTCCTGCTGC 
AGACAGGAGC TTGAGGAGCA AGTTGTCTTG CTGAATTCAT CAATGAAGAG ACCAAAATTA 
AATCCAGTGT CGGAGAGTTC CATTGCCGTG GTGACACGTT CAAGGGAGGT GTACTATTGG 
GGAGGAGGGA AGTTTACCCC CCAAAAACTT GACTCATTCA AAGCAGGCAG CGGGGCCCAG 
CATGTGTGTG CAGGAGAGAA TCATTTTGCA GTGGTGACGG TGGAGAAGGA GCTGTACACC 
TGGGCTGATG TCCAAGGTGG GGCCAAAATG GTGGGGCAGC TGGGACATGG GGATCAGGCC 
TCCTATCGAC AGCCGAAGCG GGTGGAGAAG CTCCAGGGGA AGGCCATCCG GCAGGTGGCG 
TGTGGAGCGG ACTTTACTGC CTGTGTGACT GATGAAGACC AGATGTACAT GTTCGGGTCA 
GACTATGACG GTTGCATCGG GGTTGAGGGT CAGCTGGGGT CGCAGGTTTT GGAGCCAGTT 
CTACTGGAGT TTTTTGAGGG GCGACCTGTT CAGCTGGTTT CGTGTGGAGA CAACCATGTG 
GTGGTGTTGA CCCGGGATAA GGACATCTAC TCATGGGGCT GCGGAGAATA CGGGCGTCTT 
GGTCTGGAGA GTGAGGACGA ATTTTCCTCT CCGATGCAAG TAAAGGTTCC TATAGGTGCC 
AGTATTTCCT CCGTCTCATG TGGCAGCGAT AGCACCTTCT TCTTGACAGA GTCTGGGAAA 
GTGCTAGCGT GCGGAAACAA CGAGTTCAAC AAACTGGGGT TAAATCAGGG CATCTCTGGA 
ATCAAGAATC ATCCCGGAGA GGGTTACCAA GGGATACCGT ACACCACCGC AATGACCCTG 
GTGAAACAGC TGTCACGCTT CAAGATCCAG TCCATTTCTC CAGGGAAGAC TCACACTGCT 
GCTATTGATG AACGAGGTCG TCTGATCACG TTTGGTTGCA ACAAATTTGG ACAGCTGGGT 
GTGAAAGACT TCAAGAAGCA CGCTGGTGTC CAAGTCCTTG TCGGTCCATT TGGAGGCAAG 
ACGGTGACCA AAGTGGCTTG TGGAGATGGA TTCACCATCG CTGCTACAGA AGACAATCAG 
ATCTTCGCAT GGGGAAATGC AGGAAATGGG AGGCTAGGAA TGCCTCCTGA TAAGGGCTTT 
GGTTCAGAGG TTTGCCCCGC CCTGCCCAGG CCCATTTTTG GTTCCCTCCA TCATGTACCC 
GACCTCTCGT GCCGCGGCTG GCACACTATT ATTATCATGG AAAAAGTCCT CAACTCCAAG 
ACCATCCGCT CAAACAGCAG TGGACTCTCA ATTGGCTTCG GCCATGACGG ATCGACATCA 
ACCGTTGATC TGGACTTGGA TCCCGGATCG GAGACCGAAT GTCGTGGCAG GAGTCTTGGA 
GGCACGCGAG AGGTTAACGT TGACGGATGC TCGATGGAGG CCCCAGTGAT GTCGGCTGCA 
AACCAGACTG GAGACAGCTC CTGTCCGCTG TGGCTTCAAA AGGAGCTTGA AGATGCAGAG 
TTCATCCCAA TGCCAGATAC GTGCGACAAC GACCAGCTGC CTTCTGTCTC CGAGAGCTTT 
ACTCGGCCAT ATGAGGATCT GAAAGAGCTC AAGGCAGCAG CAGATGCTGT CAGCACCGCA 
AAAGGCCTTT CGGCCACACG GATGAGCTTT GATACAGTCA ACGGTCTGGA TGGAGGGGAT 
GTCTGCAGAA AGGGGGAGTC TGGTGCTTGC TGTAAAGCAA GTAGAGAGGT GGCTCAGTTG 
CAAGAAATGC TTGCCCATCA AGAGAGGAGA ATTCAGATGC TGGAAAAGCA GGTCAGTGAA 
CAGAAAAAGG AGAATGAGAA GCTTTGGGCA GCCATCAACC GCACCGCTGT GCAGGAAAGG 
GGTCTTAACA GCGACAGAAA CTGCCTCTCT GCTCCTGGGG GAGGCGGAAG AAGAGGGGAA 
CTCACGAATC ACGGAGGCCG ATCTGCCGGG GCAAGCGTGT GA