NPHS1 cDNA ORF clone, Orcinus orca(killer whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPHS1
Entrez Gene ID	101287446
Full Name	NPHS1, nephrin
Gene Type	protein-coding
Organism	Orcinus orca(killer whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012538364.1	XP_012393818.1	nephrin

Pathways from BioSystems
Gene Ontology

The following NPHS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPHS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOr164647	XM_012538364.1 Latest version!	Orcinus orca nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPHS1 ( XM_012538364.1 ) cDNA ORF clone, Orcinus orca(killer whale) -> XP_012393818.1 Orcinus orca nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), mRNA.

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CloneID	OOr164647
Clone ID Related Accession (Same CDS sequence)	XM_012538364.1
Accession Version	XM_012538364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-14
Organism	Orcinus orca(killer whale)
Product	nephrin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004438623.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Orcinus orca Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGGAAAACC CAGAGAAAGA CAGAGGCAGA CGCAGGAGGC GGGCAGTTGT CCCTGTGGGA 
GCCACAGGCA GAGGACCTGG GCTGGCTCTG AGGCTCCCCA TCTGGACACC GCTGCTCCTC 
TTGGGGCTGC TGATGACAGG CCTGGCCCAG GTGCCAGTCC CTGCCTCAGC CCCGCGAGGC 
TTCTGGGCTC TGCCTGAAAA CTTGACGGTG GTGGAGGGGG CTGCCGCTGA GCTGTGGTGT 
GGGGTCAGCG CCCCTGGCAG CACAGTCCAA TGGGCCAAAG ATGGGCTGCT CCTAGGCCCT 
GATCCTAGGA TCCCCAGTTT CCCGCGGTAT CGCCTGCAAG GGGACCCTAC GAAAGGTGAA 
TTCCACCTGC ACATTGAAGC ATGTGACCTC AGCGACGACG CAGGGTATGA GTGCCAGGTC 
AGCCGCTCAG AAACAGGTCC TGAGCTCGTG TCGCCCAGAG TGATCCTCTC CATCCTGGTG 
CCCCCCAAAG TGCTTCAGCT GACCCCCGAG GCAGGGAGCA CGGTCACGTG GGTAGCTGGG 
CAGGAGTACT TGGTCAGCTG TGTGTCTGGG GATGCAAAGC CAGCACCTGA CATCACCTTC 
CTCCTGAGTG GACAAACAAT TTCTGACATC TCTGCTAATG TGAATGAGGG ATCCCAGGAG 
AAACTCTTCA CCACAGAGGC CACAGCCAGG GTGACACCCC AGAGCTCGGA TAACGGGCAG 
TTACTGGTCT GTGAGGGGTC CAGCCCAGCT TTGGACACCC CTATCAAGGC TTCAATCACC 
ATGAATGTTC TGTTCCCCCC AGGACCCCCT GTCATTGAGT GGCCAGGCCT GGACGAGGGA 
CACGCGAGGG CAGGGCAGAA CTTGGAGCTG CTGTGCATGG CCCGAGGGGG GAATCCGCTA 
GCCACACTGC AGTGGCTGAA GAACGGCCAG CCAGTGTCCA CAGCCTGGGG CACAGAGCAC 
ACTCAGGCAG TGGCCCGCAG TATGTTAGTG ATGATCGTGC GGCCGGAAGA CCATGGGGCA 
AGGCTCAGCT GCGAGGCCTA CAACAGCGTA TCTACAGGGA TCCAGGAACG CGGGGTCACG 
CTGCAGGTCA CCTTTCCCCC CAGTGCCATT ACCATCCTGG GATCTGCATC CCAGTCTGAG 
AACAAGAACG TGACACTCTC CTGCATCACC AAGTCCAGTC GCCCAAGGGT CCTACTGCGA 
TGGTGGCTGG GCTGGCGGCA GCTGCTGTCC ACAGAGGAGA CGGTCATGGA TGGCCTGCAT 
GGTGGCCACA TCTCCATGTC TAACCTGACA TTCCTGGCGC GACGGGAGGA CAATGGTCTG 
ACCCTCACTT GTGAGGCCTT TAGTGAGGCC TTCACCAAGG AGACCTTCAA GAAGTCACTC 
GCCCTGAATG TGAAATATCC TGCCCAGAAG CTATGGATTG AGGGCCCCCC AGAGGGGCAG 
CGTCTCCGGG CTGGGACCCG GGTGAGGCTG GTATGTTTGG CCATTGGAGG CAATCCAGAC 
CCTTCCCTCA CCTGGTACAA GGACTCGCGC ACGGTGACCG AGCCGCGGCC ACCCCAGGAG 
CCACGGCGGG TGCAGCTGGG AAGTCTGGAG AAGTCCGGGA GCACCTTCTC CCGAGAACTG 
GTACTGATCA CCGGTCCGTC GGACAACCAG GCCAAGTTCA CGTGCAAAGC CGGCCAGCTC 
AGCGCGTCCA CGCAGCTTGT GGTGCAGTTC CCCCCAACTA ACGTGACGAT CCTGGCCAAC 
GCGTCGGCGC TGCGCCCCGG GGATGCCTTA AACTTGACGT GTGTCAGCGT TAGCAGCAAC 
CCTCCGGTCA ACTTGTCGTG GGACAAGGAG GGGGAGAGGC TGGAGGGTGT GGCCGCCACG 
CCCCGGAGTG CTCCATTCAA AGGCTCCGCT GCCGCTAGGA GCGTCCTTCT GAGAATGTCA 
TCCCGCGACC ACGGCCACCG CGTGACCTGC CGTGCCCACA GCACCGAGCT ACGGGAAACC 
GTGAGCGCGT TCTACCGCCT CAACGTGCTG TACCCTCCAG AGTTCCTGGG TGAGCAGGTG 
CTTATGGTGA CCGCGGTGGA GCAGGGCGAG GCACTGCTAC CCGTGTCCGT GTCTGCCAAC 
CCCGCCCCGG AGGCCTTCAA CTGGACCTTC CGCGGCTACC GCCTCAGCCC AGCTGGCGGC 
CCCCGGCATC GTATCCTGTC TGGTGGAGCT CTGCAGCTAT GGAACGTGAC CCGCGCTGAC 
GATGGCCTCT ACCAGCTGCA CTGCCAGAAC TCAGAGGGCA CCGCTGAAGC TCTCGTGAGG 
CTGGACGTAC ACTATGCTCC CATCATCCGA GCTCTCCAAG ACCCCACTGA AGTGAATATT 
GGGGGCTCTG TGGACATAGT CTGCACTGTT GATGCCAATC CCATCCTTCC TGAGATGTTC 
AACTGGGAGA GGCTGGGAGA AGAGGAGGAG GACCAGAGCC TGGATGATAT GGAGAAGATA 
TCAAAGGGGT CCACAGGGCG TCTTCGGATT CACCATGCCA AGTTGACCCA GGCTGGTGCT 
TACCAGTGCA TTGTGGACAA TGGGGTGGCA CCTCCAGCCA GAGGGCTGGT CCGTCTTGTT 
GTTAGATTTG CCCCCCACGT GGAGCATCCC TCTCCTCTAA CTAAAGTGGC TGCAGCTGGA 
GACAGCACTA GTTCTGCCAC CCTCCACTGT CGTGCCCGAG GTGTCCCCAA TATCGTCTTC 
ACTTGGACCA AAAATGGGGT CCCTCTGGAT CTCCAGGATC CCAGGTACAC AGAGCACACA 
TACCACCAGA GTGGTGTCCA CAGCAGCCTC CTGACCATTG CCAACGTGTC CGCAGCCCAG 
GACTATGCCC TCTTCACGTG CACAGCCACC AACCCCCTCG GCTCGGACCA CACCGATATT 
CAACTCGTCA GCATCAGCCG CCCTGATCCG CCATTGGGAT TGAAGGTCGT GAGCATGACC 
CCATACTCGG TGGGGCTGGA GTGGAAGCCT GGCTTTGATG GGGGCTTGCC ACAAAGTTTC 
CAAGTCAGGT ATGAGGCTCT GGGGACTCCA GGGTTTCTCT ACGTGAATGT CCTACCACCC 
CAGGCCACCG CCTTCACACT GACTGGGCTG CAGCCTTCTA CACGATACAG GGTCTGGTTG 
CTGGCCAGCA ATGCCCTGGG CGACAGTGGA ATAGCTGACA AAGGGTCCCA GATCTCAGTC 
ACCACTCCAG GTCTAGACCA ACCTTCTGGA GAACCTGACT ACCAGCTGCC CACAGAGCAG 
CCCGCAGGAC CCTCAAAGCT GCCCCTGCTG CCTGTGCTGT TTGCTGTTGG GGGTCTTCTG 
CTGCTTTCTA ACGCCTCCTG TGTCGGGGGA TTCCTCTGGC AGCGGAGACT GAAGCGTCTT 
GCTGAGGGCA TCTCAGAGAA GACAGAGGCA GGGTCGGAGG AAGACCGAGT CAGGAATGAA 
TACGAGGAGA GCCAGTGGAC TGGAGACCAT GACACTCGAA GCTCCATGGT TAGCACAACA 
GATGTAGAGC CATATTACCA CTCCATGAGG GACTTCAGCC CCCAGCTTCC CCCCACACTG 
GAGGAGGTGT CTTATCCCCG AGGTGTCACA GGTCTTAAGG AGGAGAATAT GGCTTTTCCT 
GGGCACGTGT ATGATGAGCT GGAAAGACTG TACGCCCCGT CTGGGGCCTG GGGACCCCTC 
TATGATGAAG TACCAATGGG CTCCTGTGAC CTCTACTGGC CTGAAGACAG GTATGAAGAT 
GCAAGAGGAA TCTATGATCA GGTGGCAGGA GATTTGGACC CTATGGAACC TGATGCTCTA 
CCCTTTGAGC TGAGGGGACA GCTGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012393818.1
CDS	1..3810
Translation	MENPEKDRGRRRRRAVVPVGATGRGPGLALRLPIWTPLLLLGLLMTGLAQVPVPASAPRGFWALPENLTVVEGAAAELWCGVSAPGSTVQWAKDGLLLGPDPRIPSFPRYRLQGDPTKGEFHLHIEACDLSDDAGYECQVSRSETGPELVSPRVILSILVPPKVLQLTPEAGSTVTWVAGQEYLVSCVSGDAKPAPDITFLLSGQTISDISANVNEGSQEKLFTTEATARVTPQSSDNGQLLVCEGSSPALDTPIKASITMNVLFPPGPPVIEWPGLDEGHARAGQNLELLCMARGGNPLATLQWLKNGQPVSTAWGTEHTQAVARSMLVMIVRPEDHGARLSCEAYNSVSTGIQERGVTLQVTFPPSAITILGSASQSENKNVTLSCITKSSRPRVLLRWWLGWRQLLSTEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLALNVKYPAQKLWIEGPPEGQRLRAGTRVRLVCLAIGGNPDPSLTWYKDSRTVTEPRPPQEPRRVQLGSLEKSGSTFSRELVLITGPSDNQAKFTCKAGQLSASTQLVVQFPPTNVTILANASALRPGDALNLTCVSVSSNPPVNLSWDKEGERLEGVAATPRSAPFKGSAAARSVLLRMSSRDHGHRVTCRAHSTELRETVSAFYRLNVLYPPEFLGEQVLMVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAEALVRLDVHYAPIIRALQDPTEVNIGGSVDIVCTVDANPILPEMFNWERLGEEEEDQSLDDMEKISKGSTGRLRIHHAKLTQAGAYQCIVDNGVAPPARGLVRLVVRFAPHVEHPSPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPLDLQDPRYTEHTYHQSGVHSSLLTIANVSAAQDYALFTCTATNPLGSDHTDIQLVSISRPDPPLGLKVVSMTPYSVGLEWKPGFDGGLPQSFQVRYEALGTPGFLYVNVLPPQATAFTLTGLQPSTRYRVWLLASNALGDSGIADKGSQISVTTPGLDQPSGEPDYQLPTEQPAGPSKLPLLPVLFAVGGLLLLSNASCVGGFLWQRRLKRLAEGISEKTEAGSEEDRVRNEYEESQWTGDHDTRSSMVSTTDVEPYYHSMRDFSPQLPPTLEEVSYPRGVTGLKEENMAFPGHVYDELERLYAPSGAWGPLYDEVPMGSCDLYWPEDRYEDARGIYDQVAGDLDPMEPDALPFELRGQLV

Target ORF information:

RefSeq Version	XM_012538364.1
Organism	Orcinus orca(killer whale)
Definition	Orcinus orca nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012538364.1

ORF Insert Sequence:

ATGGAAAACC CAGAGAAAGA CAGAGGCAGA CGCAGGAGGC GGGCAGTTGT CCCTGTGGGA 
GCCACAGGCA GAGGACCTGG GCTGGCTCTG AGGCTCCCCA TCTGGACACC GCTGCTCCTC 
TTGGGGCTGC TGATGACAGG CCTGGCCCAG GTGCCAGTCC CTGCCTCAGC CCCGCGAGGC 
TTCTGGGCTC TGCCTGAAAA CTTGACGGTG GTGGAGGGGG CTGCCGCTGA GCTGTGGTGT 
GGGGTCAGCG CCCCTGGCAG CACAGTCCAA TGGGCCAAAG ATGGGCTGCT CCTAGGCCCT 
GATCCTAGGA TCCCCAGTTT CCCGCGGTAT CGCCTGCAAG GGGACCCTAC GAAAGGTGAA 
TTCCACCTGC ACATTGAAGC ATGTGACCTC AGCGACGACG CAGGGTATGA GTGCCAGGTC 
AGCCGCTCAG AAACAGGTCC TGAGCTCGTG TCGCCCAGAG TGATCCTCTC CATCCTGGTG 
CCCCCCAAAG TGCTTCAGCT GACCCCCGAG GCAGGGAGCA CGGTCACGTG GGTAGCTGGG 
CAGGAGTACT TGGTCAGCTG TGTGTCTGGG GATGCAAAGC CAGCACCTGA CATCACCTTC 
CTCCTGAGTG GACAAACAAT TTCTGACATC TCTGCTAATG TGAATGAGGG ATCCCAGGAG 
AAACTCTTCA CCACAGAGGC CACAGCCAGG GTGACACCCC AGAGCTCGGA TAACGGGCAG 
TTACTGGTCT GTGAGGGGTC CAGCCCAGCT TTGGACACCC CTATCAAGGC TTCAATCACC 
ATGAATGTTC TGTTCCCCCC AGGACCCCCT GTCATTGAGT GGCCAGGCCT GGACGAGGGA 
CACGCGAGGG CAGGGCAGAA CTTGGAGCTG CTGTGCATGG CCCGAGGGGG GAATCCGCTA 
GCCACACTGC AGTGGCTGAA GAACGGCCAG CCAGTGTCCA CAGCCTGGGG CACAGAGCAC 
ACTCAGGCAG TGGCCCGCAG TATGTTAGTG ATGATCGTGC GGCCGGAAGA CCATGGGGCA 
AGGCTCAGCT GCGAGGCCTA CAACAGCGTA TCTACAGGGA TCCAGGAACG CGGGGTCACG 
CTGCAGGTCA CCTTTCCCCC CAGTGCCATT ACCATCCTGG GATCTGCATC CCAGTCTGAG 
AACAAGAACG TGACACTCTC CTGCATCACC AAGTCCAGTC GCCCAAGGGT CCTACTGCGA 
TGGTGGCTGG GCTGGCGGCA GCTGCTGTCC ACAGAGGAGA CGGTCATGGA TGGCCTGCAT 
GGTGGCCACA TCTCCATGTC TAACCTGACA TTCCTGGCGC GACGGGAGGA CAATGGTCTG 
ACCCTCACTT GTGAGGCCTT TAGTGAGGCC TTCACCAAGG AGACCTTCAA GAAGTCACTC 
GCCCTGAATG TGAAATATCC TGCCCAGAAG CTATGGATTG AGGGCCCCCC AGAGGGGCAG 
CGTCTCCGGG CTGGGACCCG GGTGAGGCTG GTATGTTTGG CCATTGGAGG CAATCCAGAC 
CCTTCCCTCA CCTGGTACAA GGACTCGCGC ACGGTGACCG AGCCGCGGCC ACCCCAGGAG 
CCACGGCGGG TGCAGCTGGG AAGTCTGGAG AAGTCCGGGA GCACCTTCTC CCGAGAACTG 
GTACTGATCA CCGGTCCGTC GGACAACCAG GCCAAGTTCA CGTGCAAAGC CGGCCAGCTC 
AGCGCGTCCA CGCAGCTTGT GGTGCAGTTC CCCCCAACTA ACGTGACGAT CCTGGCCAAC 
GCGTCGGCGC TGCGCCCCGG GGATGCCTTA AACTTGACGT GTGTCAGCGT TAGCAGCAAC 
CCTCCGGTCA ACTTGTCGTG GGACAAGGAG GGGGAGAGGC TGGAGGGTGT GGCCGCCACG 
CCCCGGAGTG CTCCATTCAA AGGCTCCGCT GCCGCTAGGA GCGTCCTTCT GAGAATGTCA 
TCCCGCGACC ACGGCCACCG CGTGACCTGC CGTGCCCACA GCACCGAGCT ACGGGAAACC 
GTGAGCGCGT TCTACCGCCT CAACGTGCTG TACCCTCCAG AGTTCCTGGG TGAGCAGGTG 
CTTATGGTGA CCGCGGTGGA GCAGGGCGAG GCACTGCTAC CCGTGTCCGT GTCTGCCAAC 
CCCGCCCCGG AGGCCTTCAA CTGGACCTTC CGCGGCTACC GCCTCAGCCC AGCTGGCGGC 
CCCCGGCATC GTATCCTGTC TGGTGGAGCT CTGCAGCTAT GGAACGTGAC CCGCGCTGAC 
GATGGCCTCT ACCAGCTGCA CTGCCAGAAC TCAGAGGGCA CCGCTGAAGC TCTCGTGAGG 
CTGGACGTAC ACTATGCTCC CATCATCCGA GCTCTCCAAG ACCCCACTGA AGTGAATATT 
GGGGGCTCTG TGGACATAGT CTGCACTGTT GATGCCAATC CCATCCTTCC TGAGATGTTC 
AACTGGGAGA GGCTGGGAGA AGAGGAGGAG GACCAGAGCC TGGATGATAT GGAGAAGATA 
TCAAAGGGGT CCACAGGGCG TCTTCGGATT CACCATGCCA AGTTGACCCA GGCTGGTGCT 
TACCAGTGCA TTGTGGACAA TGGGGTGGCA CCTCCAGCCA GAGGGCTGGT CCGTCTTGTT 
GTTAGATTTG CCCCCCACGT GGAGCATCCC TCTCCTCTAA CTAAAGTGGC TGCAGCTGGA 
GACAGCACTA GTTCTGCCAC CCTCCACTGT CGTGCCCGAG GTGTCCCCAA TATCGTCTTC 
ACTTGGACCA AAAATGGGGT CCCTCTGGAT CTCCAGGATC CCAGGTACAC AGAGCACACA 
TACCACCAGA GTGGTGTCCA CAGCAGCCTC CTGACCATTG CCAACGTGTC CGCAGCCCAG 
GACTATGCCC TCTTCACGTG CACAGCCACC AACCCCCTCG GCTCGGACCA CACCGATATT 
CAACTCGTCA GCATCAGCCG CCCTGATCCG CCATTGGGAT TGAAGGTCGT GAGCATGACC 
CCATACTCGG TGGGGCTGGA GTGGAAGCCT GGCTTTGATG GGGGCTTGCC ACAAAGTTTC 
CAAGTCAGGT ATGAGGCTCT GGGGACTCCA GGGTTTCTCT ACGTGAATGT CCTACCACCC 
CAGGCCACCG CCTTCACACT GACTGGGCTG CAGCCTTCTA CACGATACAG GGTCTGGTTG 
CTGGCCAGCA ATGCCCTGGG CGACAGTGGA ATAGCTGACA AAGGGTCCCA GATCTCAGTC 
ACCACTCCAG GTCTAGACCA ACCTTCTGGA GAACCTGACT ACCAGCTGCC CACAGAGCAG 
CCCGCAGGAC CCTCAAAGCT GCCCCTGCTG CCTGTGCTGT TTGCTGTTGG GGGTCTTCTG 
CTGCTTTCTA ACGCCTCCTG TGTCGGGGGA TTCCTCTGGC AGCGGAGACT GAAGCGTCTT 
GCTGAGGGCA TCTCAGAGAA GACAGAGGCA GGGTCGGAGG AAGACCGAGT CAGGAATGAA 
TACGAGGAGA GCCAGTGGAC TGGAGACCAT GACACTCGAA GCTCCATGGT TAGCACAACA 
GATGTAGAGC CATATTACCA CTCCATGAGG GACTTCAGCC CCCAGCTTCC CCCCACACTG 
GAGGAGGTGT CTTATCCCCG AGGTGTCACA GGTCTTAAGG AGGAGAATAT GGCTTTTCCT 
GGGCACGTGT ATGATGAGCT GGAAAGACTG TACGCCCCGT CTGGGGCCTG GGGACCCCTC 
TATGATGAAG TACCAATGGG CTCCTGTGAC CTCTACTGGC CTGAAGACAG GTATGAAGAT 
GCAAGAGGAA TCTATGATCA GGTGGCAGGA GATTTGGACC CTATGGAACC TGATGCTCTA 
CCCTTTGAGC TGAGGGGACA GCTGGTGTGA