VARS cDNA ORF clone, Orcinus orca(killer whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	VARS
Entrez Gene ID	101284813
Full Name	valyl-tRNA synthetase
Gene Type	protein-coding
Organism	Orcinus orca(killer whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004286583.2	XP_004286631.1	valine--tRNA ligase

Pathways from BioSystems
Gene Ontology

The following VARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOr167184	XM_004286583.2 Latest version!	Orcinus orca valyl-tRNA synthetase (VARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VARS ( XM_004286583.2 ) cDNA ORF clone, Orcinus orca(killer whale) -> XP_004286631.1 Orcinus orca valyl-tRNA synthetase (VARS), mRNA.

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CloneID	OOr167184
Clone ID Related Accession (Same CDS sequence)	XM_004286583.2
Accession Version	XM_004286583.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-14
Organism	Orcinus orca(killer whale)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004438747.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_004286583.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Orcinus orca Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGTTC TCTACGTCTC TCCTCACCCC GATGCCTTCC CCAGCCTCCG AGCCCTTATA 
GCTGCTCGCT ACGGGGAGCA TGGGGAGGGT CCGGGATGGG GAGGAGCCCA CCCCCGCATC 
TGTCTCCAGC CGCCCCCGAC CAGCAGGAGT CCCTTTCCGC CACCCCGCCT GCCCGCCCTG 
GAACAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACTG CTGTGGCCCA GCTGCTGTGG 
CCAGCAGGCC TGGGGGGCCC TGGGGGTAGT CGGGCAGCCG TCCTCGTCCA ACAGTGGGTC 
AGTTACGCAG ACACGGAGCT GATACCAGCT GCCTGTGGGG CAACGCTGCC AGCCTTGGGA 
CTCCGAAGCT CTGGCCAGGA CCCCCAGGCT GCACTTGCGG CCCTGGGCAG GGCCCTGAGC 
CCCTTGGAAG AGTGGCTTCG GCTGCACACC TACTTGGCTG GAGAGGCTCC CACTCTGGCT 
GACCTGGCGG CTGTCACAGC TTTGCTGCTG CCTTTCCGTT ATGTCCTGGA CCCCTCTGCC 
CGCCGGATTT GGGGTAATGT GACTCGCTGG TTTATCACAT GTGTCCAGCA GCCAGAATTC 
CGGGCCGTGC TGGGAGAGGT GGCTGTGTAT TCAGGGGCCA GGCCTCTCTC CCAACAGCCA 
GGCTCTGAGG TCCCTGCACC CCCAAAGACA GCTGCTCAAC TCAAGAAAGA GGCAAAGAAA 
CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGGTCC AACAGCAGCA GCCACCCCCT 
GGGGAGAAGA AGCCAAAACC AGAGAAGAGG GAGAAACGGG ATCCTGGGGT CATTACCTAT 
GACCTCCCAA CCCCACCTGG AGAAAAGAAA GATGTCAGTG GCACCATGCC TGACTCCTAC 
AGCCCTCAGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCAGCAGGG CTTCTTCAGG 
CCCGAGTATG GGCGTTCCAG TGTGTCAGCA CCAAATCCTC GGGGCATCTT CATGATGTGC 
ATTCCACCCC CCAACGTGAC AGGCTCTCTG CACCTGGGCC ACGCGCTCAC CAACGCCATC 
CAGGACTCCC TGACCCGATG GCACCGCATG CGCGGGGAGA CCACCCTGTG GAATCCTGGC 
TGTGACCACG CGGGCATTGC CACCCAGGTG GTGGTGGAGA AGAAACTCTG GCGCGAGCAG 
GGGCTGAGTC GGCACCAGCT GGGCCGAGAG GCCTTTCTGC GGGAGGTCTG GAAGTGGAAG 
GAGGAGAAAG GTGACCGGAT TTACCACCAG CTGAAGAAGC TTGGCAGCTC CTTGGACTGG 
AATCGAGCCT GTTTCACCAT GGATCCTAAA CTGTCAGCAG CCGTGACGGA GGCCTTTGTC 
CAGCTCCATG AGGAAGGAGT CATCTACCGT AGTACCCGCC TTGTCAACTG GTCCTGCACC 
CTCAACTCTG CCATCTCGGA CATCGAGGTG GATAAGAAGG AGTTGACAGG CCGCACACTG 
CTCTCTGTGC CTGGCTACAA GGAGAAGGTG GAGTTTGGGG TCCTCGTCTC CTTTGCTTAC 
AAGGTCCAAG GCTCAGACAG CGACGAGGAG GTGCTGGTGG CAACAACTCG GATTGAGACG 
ATGCTGGGAG ACGTGGCCGT AGCCGTGCAC CCCAAAGACC CCAGATACCA GCACCTGAAG 
GGGAAGAGCG TGACCCACCC GTTCGTGTCT CGGAGCCTCC CCGTCATCTT CGATGATTTT 
GTGGACATGG AGTTCGGCAC AGGCGCGGTG AAGATCACCC CTGCACACGA CCAGAATGAC 
TATGAGGTTG GGCAGCGGCA TGGGCTGGAG GCCATCAGCA TCATGGACGC CCAAGGGGCC 
CTTGTCAACG TGCCCCCACC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG AAAGGCAGTG 
TTGGCGGCGC TGAAGGAGCG GGGACTGTTC CGTGGCACTG AGGACAACCC CATGGTGGTG 
CCACTTTGCA ACCGCTCCAA GGATGTGGTG GAGCCTCTTC TGCGGCCGCA GTGGTACGTG 
CGCTGTGGGG AGATGGCCCA GGCCGCCAGC GCTGCTGTGA CACGGGGCGA CCTCCGCATC 
CTGCCCGAGG CCCATCAACG CACATGGCAT GCCTGGATGG ACAACATCAG GGACTGGTGC 
ATCTCCCGGC AGCTGTGGTG GGGCCATCGC ATTCCAGCCT ATTTCGTCAC TGTCAGTGAC 
CCAGCTGTAC CCCCAGGGGA GGACCCTGAT GGGCGGTACT GGGTGAGCGG GCGCACCAAG 
GCCGAGGCTC TGGAGAAGGC AGCCGAGGAG TTCGGAGTGT CCCCGGACAA GATCAGTCTC 
CAGCAAGATG AAGACGTACT GGACACCTGG TTCTCCTCTG GCCTCTTCCC CTTCTCCATC 
CTGGGCTGGC CCAACCAGTC GGAAGATCTG AGTGTGTTCT ACCCGGGGAC GCTGCTGGAG 
ACGGGCCACG ATATCCTCTT CTTCTGGGTG GCCAGGATGG TCATGCTTGG CCTGAAGCTC 
ACTGGCAGGC TGCCCTTCAG AGAGGTCTAC CTCCACGCCA TCGTGCGGGA TGCCCACGGC 
CGGAAGATGA GCAAGTCTCT AGGCAACGTC ATTGACCCCC TGGACGTTAT CCACGGAGTC 
TCTCTGCAGG GCCTCCACGA CCAGTTACTG AACAGCAACC TGGATCCCAG CGAGGTGGAA 
AAGGCTAAAG AAGGGCAGAA GGCAGATTTC CCGGCGGGGA TCCCTGAGTG TGGCACCGAT 
GCTCTCCGGT TTGGACTGTG CGCCTACACG TCCCAGGGCC GTGACATCAA CCTGGATGTG 
AACCGGATAT TGGGATACCG CCATTTCTGC AACAAGCTCT GGAACGCCAC CAAGTTTGCC 
CTCCGTGGCC TTGGGAAGGA TTTTGTGCCC TCACCCACCT CTGAGCCTGG AGGCCGCGGG 
AGCCTGGTGG ACCGCTGGAT CCGCAGCCGG CTGACCGAAG CTGTGAGGCT CAGCAACCAG 
GGTTTCCAGG CCTACGACTT CCCAGCTGCC ACCACCGCCC AGTACAGCTT CTGGCTCTAC 
GAGCTCTGTG ATGTGTACCT GGAGTGCCTG AAGCCTGTGC TGAGTGGGGT GGACCAGGTG 
GCGGCCGAGC ACGCCCGCCA GACCCTCTAC ACCTGCCTGG ACGTCGGCCT GCGGCTGCTC 
TCGCCCTTCA TGCCCTTTGT GACCGAGGAG CTGTTCCAGA GGCTGCCCCG GCGGACGCCA 
CAAGCTCCCC CTAGCCTCTG CGTTACCTCC TACCCAGAGC CCTCGGAGTG CTCCTGGAAG 
GACCCTGAGG CGGAAGGCGC CTTCGAGCTG GCCCTGAGCA TCACTCGAGC CGTGCGCTCC 
CTGCGTGCCG ACTACAACCT CACCCGGATC CGGCCCGACT GTTTCCTGGA AGTGGCTGAT 
GAGGCCACAG GCGCCCTGGC ATCAGCAGTG TCGGGCTATG TGCAGACGCT GGCCAGCGCG 
GGTATAGTGG CTGTCCTGGC GCTGGGGGCT TCTGCACCCC AGGGCTGCGC TGTGGCCCTG 
GCTTCTGACC GCTGCTCTGT CCACCTGCAG CTGCAGGGCC TGGTAGACCC GGCCCGGGAG 
CTGGGCAAAC TGCAGGCCAA GCGCAGTGAG GCGCAGCGGC AGGCCCAGCG TCTACGGGAA 
CGCCGCGCTG CCTCGGGCTA CACTGTCAAG GTGCCCCTCA AAGTCCAGGA GGCAGATGAA 
GCCAAGCTCC AGCAGACAGA AGCAGAGCTC AGGAAGGTCG ATGAGGCCAT CTCCCTATTT 
CAGAAGATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004286631.1
CDS	190..3984
Translation	MSVLYVSPHPDAFPSLRALIAARYGEHGEGPGWGGAHPRICLQPPPTSRSPFPPPRLPALEQGPGGLWVWGATAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRSSGQDPQAALAALGRALSPLEEWLRLHTYLAGEAPTLADLAAVTALLLPFRYVLDPSARRIWGNVTRWFITCVQQPEFRAVLGEVAVYSGARPLSQQPGSEVPAPPKTAAQLKKEAKKREKLEKFQQKQKVQQQQPPPGEKKPKPEKREKRDPGVITYDLPTPPGEKKDVSGTMPDSYSPQYVEAAWYPWWEQQGFFRPEYGRSSVSAPNPRGIFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLREVWKWKEEKGDRIYHQLKKLGSSLDWNRACFTMDPKLSAAVTEAFVQLHEEGVIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSDEEVLVATTRIETMLGDVAVAVHPKDPRYQHLKGKSVTHPFVSRSLPVIFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHGLEAISIMDAQGALVNVPPPFLGLPRFEARKAVLAALKERGLFRGTEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIRDWCISRQLWWGHRIPAYFVTVSDPAVPPGEDPDGRYWVSGRTKAEALEKAAEEFGVSPDKISLQQDEDVLDTWFSSGLFPFSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGRLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLHDQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKDFVPSPTSEPGGRGSLVDRWIRSRLTEAVRLSNQGFQAYDFPAATTAQYSFWLYELCDVYLECLKPVLSGVDQVAAEHARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRTPQAPPSLCVTSYPEPSECSWKDPEAEGAFELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQTLASAGIVAVLALGASAPQGCAVALASDRCSVHLQLQGLVDPARELGKLQAKRSEAQRQAQRLRERRAASGYTVKVPLKVQEADEAKLQQTEAELRKVDEAISLFQKML

Target ORF information:

RefSeq Version	XM_004286583.2
Organism	Orcinus orca(killer whale)
Definition	Orcinus orca valyl-tRNA synthetase (VARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004286583.2

ORF Insert Sequence:

ATGTCCGTTC TCTACGTCTC TCCTCACCCC GATGCCTTCC CCAGCCTCCG AGCCCTTATA 
GCTGCTCGCT ACGGGGAGCA TGGGGAGGGT CCGGGATGGG GAGGAGCCCA CCCCCGCATC 
TGTCTCCAGC CGCCCCCGAC CAGCAGGAGT CCCTTTCCGC CACCCCGCCT GCCCGCCCTG 
GAACAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACTG CTGTGGCCCA GCTGCTGTGG 
CCAGCAGGCC TGGGGGGCCC TGGGGGTAGT CGGGCAGCCG TCCTCGTCCA ACAGTGGGTC 
AGTTACGCAG ACACGGAGCT GATACCAGCT GCCTGTGGGG CAACGCTGCC AGCCTTGGGA 
CTCCGAAGCT CTGGCCAGGA CCCCCAGGCT GCACTTGCGG CCCTGGGCAG GGCCCTGAGC 
CCCTTGGAAG AGTGGCTTCG GCTGCACACC TACTTGGCTG GAGAGGCTCC CACTCTGGCT 
GACCTGGCGG CTGTCACAGC TTTGCTGCTG CCTTTCCGTT ATGTCCTGGA CCCCTCTGCC 
CGCCGGATTT GGGGTAATGT GACTCGCTGG TTTATCACAT GTGTCCAGCA GCCAGAATTC 
CGGGCCGTGC TGGGAGAGGT GGCTGTGTAT TCAGGGGCCA GGCCTCTCTC CCAACAGCCA 
GGCTCTGAGG TCCCTGCACC CCCAAAGACA GCTGCTCAAC TCAAGAAAGA GGCAAAGAAA 
CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGGTCC AACAGCAGCA GCCACCCCCT 
GGGGAGAAGA AGCCAAAACC AGAGAAGAGG GAGAAACGGG ATCCTGGGGT CATTACCTAT 
GACCTCCCAA CCCCACCTGG AGAAAAGAAA GATGTCAGTG GCACCATGCC TGACTCCTAC 
AGCCCTCAGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCAGCAGGG CTTCTTCAGG 
CCCGAGTATG GGCGTTCCAG TGTGTCAGCA CCAAATCCTC GGGGCATCTT CATGATGTGC 
ATTCCACCCC CCAACGTGAC AGGCTCTCTG CACCTGGGCC ACGCGCTCAC CAACGCCATC 
CAGGACTCCC TGACCCGATG GCACCGCATG CGCGGGGAGA CCACCCTGTG GAATCCTGGC 
TGTGACCACG CGGGCATTGC CACCCAGGTG GTGGTGGAGA AGAAACTCTG GCGCGAGCAG 
GGGCTGAGTC GGCACCAGCT GGGCCGAGAG GCCTTTCTGC GGGAGGTCTG GAAGTGGAAG 
GAGGAGAAAG GTGACCGGAT TTACCACCAG CTGAAGAAGC TTGGCAGCTC CTTGGACTGG 
AATCGAGCCT GTTTCACCAT GGATCCTAAA CTGTCAGCAG CCGTGACGGA GGCCTTTGTC 
CAGCTCCATG AGGAAGGAGT CATCTACCGT AGTACCCGCC TTGTCAACTG GTCCTGCACC 
CTCAACTCTG CCATCTCGGA CATCGAGGTG GATAAGAAGG AGTTGACAGG CCGCACACTG 
CTCTCTGTGC CTGGCTACAA GGAGAAGGTG GAGTTTGGGG TCCTCGTCTC CTTTGCTTAC 
AAGGTCCAAG GCTCAGACAG CGACGAGGAG GTGCTGGTGG CAACAACTCG GATTGAGACG 
ATGCTGGGAG ACGTGGCCGT AGCCGTGCAC CCCAAAGACC CCAGATACCA GCACCTGAAG 
GGGAAGAGCG TGACCCACCC GTTCGTGTCT CGGAGCCTCC CCGTCATCTT CGATGATTTT 
GTGGACATGG AGTTCGGCAC AGGCGCGGTG AAGATCACCC CTGCACACGA CCAGAATGAC 
TATGAGGTTG GGCAGCGGCA TGGGCTGGAG GCCATCAGCA TCATGGACGC CCAAGGGGCC 
CTTGTCAACG TGCCCCCACC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG AAAGGCAGTG 
TTGGCGGCGC TGAAGGAGCG GGGACTGTTC CGTGGCACTG AGGACAACCC CATGGTGGTG 
CCACTTTGCA ACCGCTCCAA GGATGTGGTG GAGCCTCTTC TGCGGCCGCA GTGGTACGTG 
CGCTGTGGGG AGATGGCCCA GGCCGCCAGC GCTGCTGTGA CACGGGGCGA CCTCCGCATC 
CTGCCCGAGG CCCATCAACG CACATGGCAT GCCTGGATGG ACAACATCAG GGACTGGTGC 
ATCTCCCGGC AGCTGTGGTG GGGCCATCGC ATTCCAGCCT ATTTCGTCAC TGTCAGTGAC 
CCAGCTGTAC CCCCAGGGGA GGACCCTGAT GGGCGGTACT GGGTGAGCGG GCGCACCAAG 
GCCGAGGCTC TGGAGAAGGC AGCCGAGGAG TTCGGAGTGT CCCCGGACAA GATCAGTCTC 
CAGCAAGATG AAGACGTACT GGACACCTGG TTCTCCTCTG GCCTCTTCCC CTTCTCCATC 
CTGGGCTGGC CCAACCAGTC GGAAGATCTG AGTGTGTTCT ACCCGGGGAC GCTGCTGGAG 
ACGGGCCACG ATATCCTCTT CTTCTGGGTG GCCAGGATGG TCATGCTTGG CCTGAAGCTC 
ACTGGCAGGC TGCCCTTCAG AGAGGTCTAC CTCCACGCCA TCGTGCGGGA TGCCCACGGC 
CGGAAGATGA GCAAGTCTCT AGGCAACGTC ATTGACCCCC TGGACGTTAT CCACGGAGTC 
TCTCTGCAGG GCCTCCACGA CCAGTTACTG AACAGCAACC TGGATCCCAG CGAGGTGGAA 
AAGGCTAAAG AAGGGCAGAA GGCAGATTTC CCGGCGGGGA TCCCTGAGTG TGGCACCGAT 
GCTCTCCGGT TTGGACTGTG CGCCTACACG TCCCAGGGCC GTGACATCAA CCTGGATGTG 
AACCGGATAT TGGGATACCG CCATTTCTGC AACAAGCTCT GGAACGCCAC CAAGTTTGCC 
CTCCGTGGCC TTGGGAAGGA TTTTGTGCCC TCACCCACCT CTGAGCCTGG AGGCCGCGGG 
AGCCTGGTGG ACCGCTGGAT CCGCAGCCGG CTGACCGAAG CTGTGAGGCT CAGCAACCAG 
GGTTTCCAGG CCTACGACTT CCCAGCTGCC ACCACCGCCC AGTACAGCTT CTGGCTCTAC 
GAGCTCTGTG ATGTGTACCT GGAGTGCCTG AAGCCTGTGC TGAGTGGGGT GGACCAGGTG 
GCGGCCGAGC ACGCCCGCCA GACCCTCTAC ACCTGCCTGG ACGTCGGCCT GCGGCTGCTC 
TCGCCCTTCA TGCCCTTTGT GACCGAGGAG CTGTTCCAGA GGCTGCCCCG GCGGACGCCA 
CAAGCTCCCC CTAGCCTCTG CGTTACCTCC TACCCAGAGC CCTCGGAGTG CTCCTGGAAG 
GACCCTGAGG CGGAAGGCGC CTTCGAGCTG GCCCTGAGCA TCACTCGAGC CGTGCGCTCC 
CTGCGTGCCG ACTACAACCT CACCCGGATC CGGCCCGACT GTTTCCTGGA AGTGGCTGAT 
GAGGCCACAG GCGCCCTGGC ATCAGCAGTG TCGGGCTATG TGCAGACGCT GGCCAGCGCG 
GGTATAGTGG CTGTCCTGGC GCTGGGGGCT TCTGCACCCC AGGGCTGCGC TGTGGCCCTG 
GCTTCTGACC GCTGCTCTGT CCACCTGCAG CTGCAGGGCC TGGTAGACCC GGCCCGGGAG 
CTGGGCAAAC TGCAGGCCAA GCGCAGTGAG GCGCAGCGGC AGGCCCAGCG TCTACGGGAA 
CGCCGCGCTG CCTCGGGCTA CACTGTCAAG GTGCCCCTCA AAGTCCAGGA GGCAGATGAA 
GCCAAGCTCC AGCAGACAGA AGCAGAGCTC AGGAAGGTCG ATGAGGCCAT CTCCCTATTT 
CAGAAGATGC TGTGA