NFASC cDNA ORF clone, Orcinus orca(killer whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFASC
Entrez Gene ID	101280608
Full Name	neurofascin
Gene Type	protein-coding
Organism	Orcinus orca(killer whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004282457.1	XP_004282505.1	neurofascin isoform X2
XM_004282458.1	XP_004282506.1	neurofascin isoform X3
XM_004282459.1	XP_004282507.1	neurofascin isoform X4
XM_004282460.2	XP_004282508.1	neurofascin isoform X5
XM_004282461.1	XP_004282509.1	neurofascin isoform X6
XM_012537675.1	XP_012393129.1	neurofascin isoform X1
XM_012537676.1	XP_012393130.1	neurofascin isoform X6

Pathways from BioSystems
Gene Ontology

The following NFASC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFASC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOr162782	XM_004282461.1 Latest version!	Orcinus orca neurofascin (NFASC), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OOr162782	XM_012537676.1 Latest version!	Orcinus orca neurofascin (NFASC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OOr162777	XM_012537675.1 Latest version!	Orcinus orca neurofascin (NFASC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OOr162778	XM_004282457.1 Latest version!	Orcinus orca neurofascin (NFASC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr162779	XM_004282458.1 Latest version!	Orcinus orca neurofascin (NFASC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr162780	XM_004282459.1 Latest version!	Orcinus orca neurofascin (NFASC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr162781	XM_004282460.2 Latest version!	Orcinus orca neurofascin (NFASC), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFASC ( XM_004282461.1 ) cDNA ORF clone, Orcinus orca(killer whale) -> XP_004282509.1 Orcinus orca neurofascin (NFASC), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OOr162782
Clone ID Related Accession (Same CDS sequence)	XM_004282461.1 , XM_012537676.1
Accession Version	XM_004282461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-14
Organism	Orcinus orca(killer whale)
Product	neurofascin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004438580.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Orcinus orca Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CTGTCCCAGC CCCCAACCAT CACCAAGCAG 
TCAGTGAAGG AGCACATCGT GGATCCCCGC GATAACATCT TGATTGAGTG CGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACT CGCAACAGTA GGTTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCGATGAG GAGGAGGTCT GGTACCCTGG TGATCGACTT CCGCAGTGGC 
GGGCGGCCAG AGGAGTATGA GGGGGAATAC CAGTGCTTTG CCCGCAACAA GTTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTCGTTCA AGAAGGTGCT CCCTTGACGC TCCAGTGCAA CCCCCCACCT 
GGACTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAGCGTGTCT CCCAGGGCCA TAACGGAGAC CTGTATTTCT CTAATGTGAT GCTGCAGGAC 
ATGCAGACGG ACTACAGCTG CAATGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCATTCA ACCTCAAAGT CCTTACCACC CGAGGAGTTG CAGAAAGAAC ACCCAGCTTC 
ATGTATCCCC AGGGCACTGC AAGCAGTCAG ATGGTGCTGC GTGGCATGGA CCTCCTGCTG 
GAGTGCATCG CCTCCGGGGT CCCAACACCA GACATCGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCGTCTG ACAAAGCCAA GTTTGAGAAC TTTAACAAGG CCCTGCGTAT CACCAACGTG 
TCCGAGGAAG ACTCCGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT 
ATCCTGGCTC CTGGCGAGGA CGGGAGACTG GTGTGTCGAG CCAACGGGAA CCCCAAGCCC 
ACCGTCCAGT GGATGGTGAA TGGGGAACCT TTGCAATCGG CACCACCCAA CCCAAACCGT 
GAGGTGGCCG GGGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG GGCCGTGTAC 
CAGTGCAACA CCTCCAACGA GCACGGCTAC CTGCTGGCCA ACGCCTTCGT CAGCGTGCTG 
GACGTGCCGC CTCGGATGCT GTCACCCCGG AACCAGCTCA TCAGGGTGAT CCTGTACAAC 
CGGACGCGGC TGGACTGCCC GTTCTTTGGG TCTCCCATCC CCACACTCCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGTGGC AACTACCATG TCTACGAGAA CGGCAGTCTG 
GAAATCAAGA TGATCCGCAA AGAGGACCAA GGCATCTACA CCTGTGTTGC CACCAACATC 
CTGGGCAAAG CTGAGAACCA GGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGC 
ATGCCTGAGG ACCAGGTCGC CAAAAGGGGC ACCACGGTGC AGCTGGAGTG TCGCGTGAAG 
CACGACCCCT CCCTGAAGCT CACGGTGTAC TGGCTGAAGG ACGACGAGCC CCTCTACGTC 
GGGAACAGGA TGAAGAAGGA AGAGGACTCC CTGACCATCT TTGGTGTGGC CGAGCGGGAC 
CAGGGTAGCT ACACATGCGT TGCCAGCACC GAGCTGGACC AAGACCTGGC CAAGGCCTAC 
CTGACCGTGC TAGGTAACTG CCGCTGCCCA CACTGGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004282509.1
CDS	319..2160
Translation	MARQQPPPWVHTAFLFCLLSLSGAIEIPMDLSQPPTITKQSVKEHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFNLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVYWLKDDEPLYVGNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGNCRCPHWR

Target ORF information:

RefSeq Version	XM_004282461.1
Organism	Orcinus orca(killer whale)
Definition	Orcinus orca neurofascin (NFASC), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004282461.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CTGTCCCAGC CCCCAACCAT CACCAAGCAG 
TCAGTGAAGG AGCACATCGT GGATCCCCGC GATAACATCT TGATTGAGTG CGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACT CGCAACAGTA GGTTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCGATGAG GAGGAGGTCT GGTACCCTGG TGATCGACTT CCGCAGTGGC 
GGGCGGCCAG AGGAGTATGA GGGGGAATAC CAGTGCTTTG CCCGCAACAA GTTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTCGTTCA AGAAGGTGCT CCCTTGACGC TCCAGTGCAA CCCCCCACCT 
GGACTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAGCGTGTCT CCCAGGGCCA TAACGGAGAC CTGTATTTCT CTAATGTGAT GCTGCAGGAC 
ATGCAGACGG ACTACAGCTG CAATGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCATTCA ACCTCAAAGT CCTTACCACC CGAGGAGTTG CAGAAAGAAC ACCCAGCTTC 
ATGTATCCCC AGGGCACTGC AAGCAGTCAG ATGGTGCTGC GTGGCATGGA CCTCCTGCTG 
GAGTGCATCG CCTCCGGGGT CCCAACACCA GACATCGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCGTCTG ACAAAGCCAA GTTTGAGAAC TTTAACAAGG CCCTGCGTAT CACCAACGTG 
TCCGAGGAAG ACTCCGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT 
ATCCTGGCTC CTGGCGAGGA CGGGAGACTG GTGTGTCGAG CCAACGGGAA CCCCAAGCCC 
ACCGTCCAGT GGATGGTGAA TGGGGAACCT TTGCAATCGG CACCACCCAA CCCAAACCGT 
GAGGTGGCCG GGGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG GGCCGTGTAC 
CAGTGCAACA CCTCCAACGA GCACGGCTAC CTGCTGGCCA ACGCCTTCGT CAGCGTGCTG 
GACGTGCCGC CTCGGATGCT GTCACCCCGG AACCAGCTCA TCAGGGTGAT CCTGTACAAC 
CGGACGCGGC TGGACTGCCC GTTCTTTGGG TCTCCCATCC CCACACTCCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGTGGC AACTACCATG TCTACGAGAA CGGCAGTCTG 
GAAATCAAGA TGATCCGCAA AGAGGACCAA GGCATCTACA CCTGTGTTGC CACCAACATC 
CTGGGCAAAG CTGAGAACCA GGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGC 
ATGCCTGAGG ACCAGGTCGC CAAAAGGGGC ACCACGGTGC AGCTGGAGTG TCGCGTGAAG 
CACGACCCCT CCCTGAAGCT CACGGTGTAC TGGCTGAAGG ACGACGAGCC CCTCTACGTC 
GGGAACAGGA TGAAGAAGGA AGAGGACTCC CTGACCATCT TTGGTGTGGC CGAGCGGGAC 
CAGGGTAGCT ACACATGCGT TGCCAGCACC GAGCTGGACC AAGACCTGGC CAAGGCCTAC 
CTGACCGTGC TAGGTAACTG CCGCTGCCCA CACTGGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_012537676.1 ) cDNA ORF clone, Orcinus orca(killer whale) -> XP_012393130.1 Orcinus orca neurofascin (NFASC), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OOr162782
Clone ID Related Accession (Same CDS sequence)	XM_004282461.1 , XM_012537676.1
Accession Version	XM_012537676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-14
Organism	Orcinus orca(killer whale)
Product	neurofascin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004438580.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Orcinus orca Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CTGTCCCAGC CCCCAACCAT CACCAAGCAG 
TCAGTGAAGG AGCACATCGT GGATCCCCGC GATAACATCT TGATTGAGTG CGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACT CGCAACAGTA GGTTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCGATGAG GAGGAGGTCT GGTACCCTGG TGATCGACTT CCGCAGTGGC 
GGGCGGCCAG AGGAGTATGA GGGGGAATAC CAGTGCTTTG CCCGCAACAA GTTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTCGTTCA AGAAGGTGCT CCCTTGACGC TCCAGTGCAA CCCCCCACCT 
GGACTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAGCGTGTCT CCCAGGGCCA TAACGGAGAC CTGTATTTCT CTAATGTGAT GCTGCAGGAC 
ATGCAGACGG ACTACAGCTG CAATGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCATTCA ACCTCAAAGT CCTTACCACC CGAGGAGTTG CAGAAAGAAC ACCCAGCTTC 
ATGTATCCCC AGGGCACTGC AAGCAGTCAG ATGGTGCTGC GTGGCATGGA CCTCCTGCTG 
GAGTGCATCG CCTCCGGGGT CCCAACACCA GACATCGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCGTCTG ACAAAGCCAA GTTTGAGAAC TTTAACAAGG CCCTGCGTAT CACCAACGTG 
TCCGAGGAAG ACTCCGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT 
ATCCTGGCTC CTGGCGAGGA CGGGAGACTG GTGTGTCGAG CCAACGGGAA CCCCAAGCCC 
ACCGTCCAGT GGATGGTGAA TGGGGAACCT TTGCAATCGG CACCACCCAA CCCAAACCGT 
GAGGTGGCCG GGGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG GGCCGTGTAC 
CAGTGCAACA CCTCCAACGA GCACGGCTAC CTGCTGGCCA ACGCCTTCGT CAGCGTGCTG 
GACGTGCCGC CTCGGATGCT GTCACCCCGG AACCAGCTCA TCAGGGTGAT CCTGTACAAC 
CGGACGCGGC TGGACTGCCC GTTCTTTGGG TCTCCCATCC CCACACTCCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGTGGC AACTACCATG TCTACGAGAA CGGCAGTCTG 
GAAATCAAGA TGATCCGCAA AGAGGACCAA GGCATCTACA CCTGTGTTGC CACCAACATC 
CTGGGCAAAG CTGAGAACCA GGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGC 
ATGCCTGAGG ACCAGGTCGC CAAAAGGGGC ACCACGGTGC AGCTGGAGTG TCGCGTGAAG 
CACGACCCCT CCCTGAAGCT CACGGTGTAC TGGCTGAAGG ACGACGAGCC CCTCTACGTC 
GGGAACAGGA TGAAGAAGGA AGAGGACTCC CTGACCATCT TTGGTGTGGC CGAGCGGGAC 
CAGGGTAGCT ACACATGCGT TGCCAGCACC GAGCTGGACC AAGACCTGGC CAAGGCCTAC 
CTGACCGTGC TAGGTAACTG CCGCTGCCCA CACTGGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012393130.1
CDS	298..2139
Translation	MARQQPPPWVHTAFLFCLLSLSGAIEIPMDLSQPPTITKQSVKEHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFNLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVYWLKDDEPLYVGNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGNCRCPHWR

Target ORF information:

RefSeq Version	XM_012537676.1
Organism	Orcinus orca(killer whale)
Definition	Orcinus orca neurofascin (NFASC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012537676.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CTGTCCCAGC CCCCAACCAT CACCAAGCAG 
TCAGTGAAGG AGCACATCGT GGATCCCCGC GATAACATCT TGATTGAGTG CGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACT CGCAACAGTA GGTTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCGATGAG GAGGAGGTCT GGTACCCTGG TGATCGACTT CCGCAGTGGC 
GGGCGGCCAG AGGAGTATGA GGGGGAATAC CAGTGCTTTG CCCGCAACAA GTTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTCGTTCA AGAAGGTGCT CCCTTGACGC TCCAGTGCAA CCCCCCACCT 
GGACTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAGCGTGTCT CCCAGGGCCA TAACGGAGAC CTGTATTTCT CTAATGTGAT GCTGCAGGAC 
ATGCAGACGG ACTACAGCTG CAATGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCATTCA ACCTCAAAGT CCTTACCACC CGAGGAGTTG CAGAAAGAAC ACCCAGCTTC 
ATGTATCCCC AGGGCACTGC AAGCAGTCAG ATGGTGCTGC GTGGCATGGA CCTCCTGCTG 
GAGTGCATCG CCTCCGGGGT CCCAACACCA GACATCGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCGTCTG ACAAAGCCAA GTTTGAGAAC TTTAACAAGG CCCTGCGTAT CACCAACGTG 
TCCGAGGAAG ACTCCGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT 
ATCCTGGCTC CTGGCGAGGA CGGGAGACTG GTGTGTCGAG CCAACGGGAA CCCCAAGCCC 
ACCGTCCAGT GGATGGTGAA TGGGGAACCT TTGCAATCGG CACCACCCAA CCCAAACCGT 
GAGGTGGCCG GGGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG GGCCGTGTAC 
CAGTGCAACA CCTCCAACGA GCACGGCTAC CTGCTGGCCA ACGCCTTCGT CAGCGTGCTG 
GACGTGCCGC CTCGGATGCT GTCACCCCGG AACCAGCTCA TCAGGGTGAT CCTGTACAAC 
CGGACGCGGC TGGACTGCCC GTTCTTTGGG TCTCCCATCC CCACACTCCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGTGGC AACTACCATG TCTACGAGAA CGGCAGTCTG 
GAAATCAAGA TGATCCGCAA AGAGGACCAA GGCATCTACA CCTGTGTTGC CACCAACATC 
CTGGGCAAAG CTGAGAACCA GGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGC 
ATGCCTGAGG ACCAGGTCGC CAAAAGGGGC ACCACGGTGC AGCTGGAGTG TCGCGTGAAG 
CACGACCCCT CCCTGAAGCT CACGGTGTAC TGGCTGAAGG ACGACGAGCC CCTCTACGTC 
GGGAACAGGA TGAAGAAGGA AGAGGACTCC CTGACCATCT TTGGTGTGGC CGAGCGGGAC 
CAGGGTAGCT ACACATGCGT TGCCAGCACC GAGCTGGACC AAGACCTGGC CAAGGCCTAC 
CTGACCGTGC TAGGTAACTG CCGCTGCCCA CACTGGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_012537675.1 ) cDNA ORF clone, Orcinus orca(killer whale) -> XP_012393129.1 Orcinus orca neurofascin (NFASC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOr162777
Clone ID Related Accession (Same CDS sequence)	XM_012537675.1
Accession Version	XM_012537675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-14
Organism	Orcinus orca(killer whale)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004438580.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Orcinus orca Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT GTCCCAGCCC 
CCAACCATCA CCAAGCAGTC AGTGAAGGAG CACATCGTGG ATCCCCGCGA TAACATCTTG 
ATTGAGTGCG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG CAACAGTAGG 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCGATGAGGA GGAGGTCTGG TACCCTGGTG 
ATCGACTTCC GCAGTGGCGG GCGGCCAGAG GAGTATGAGG GGGAATACCA GTGCTTTGCC 
CGCAACAAGT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTCGTTCAAG AAGGTGCTCC CTTGACGCTC 
CAGTGCAACC CCCCACCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCATA ACGGAGACCT GTATTTCTCT 
AATGTGATGC TGCAGGACAT GCAGACGGAC TACAGCTGCA ATGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCATTCAAC CTCAAAGTCC TTACCACCCG AGGAGTTGCA 
GAAAGAACAC CCAGCTTCAT GTATCCCCAG GGCACTGCAA GCAGTCAGAT GGTGCTGCGT 
GGCATGGACC TCCTGCTGGA GTGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCGTCTGAC AAAGCCAAGT TTGAGAACTT TAACAAGGCC 
CTGCGTATCA CCAACGTGTC CGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT CCTGGCTCCT GGCGAGGACG GGAGACTGGT GTGTCGAGCC 
AACGGGAACC CCAAGCCCAC CGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCCAACC CAAACCGTGA GGTGGCCGGG GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CCGTGTACCA GTGCAACACC TCCAACGAGC ACGGCTACCT GCTGGCCAAC 
GCCTTCGTCA GCGTGCTGGA CGTGCCGCCT CGGATGCTGT CACCCCGGAA CCAGCTCATC 
AGGGTGATCC TGTACAACCG GACGCGGCTG GACTGCCCGT TCTTTGGGTC TCCCATCCCC 
ACACTCCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTC 
TACGAGAACG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC 
TGTGTTGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGCAT GCCTGAGGAC CAGGTCGCCA AAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GCGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTGTACTG GCTGAAGGAC 
GACGAGCCCC TCTACGTCGG GAACAGGATG AAGAAGGAAG AGGACTCCCT GACCATCTTT 
GGTGTGGCCG AGCGGGACCA GGGTAGCTAC ACATGCGTTG CCAGCACCGA GCTGGACCAA 
GACCTGGCCA AGGCCTACCT GACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACC TGGAGCTCAC CGACCTGGCC 
GAGAGGAGCG TGAGACTGAC CTGGATTCCG GGGGACGATA ACAACAGCCC CATCACAGAC 
TACGTTGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG 
TTCCCAGGAA GTGTCAACTC AGCCATCCTC CAGCTGTCCC CCTATGTCAA CTACCAGTTC 
CGGGTCATTG CCATCAATGA GGTGGGCAGC AGCCATCCCA GCCTCCCGTC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC TGAGTCCAAC CCCTCTGATG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCG ATGAACGCCA CCTCCGCCTT TGGCCCCAAC 
CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAGACCCGAG AGACCTGGAA CAATGTCACT 
GTGTGGGGCT CCCGCTATGT GGTGGGGCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCAG AAAATGACTT TGGGAAGGGC CCTGAACCAG ACACTGTCAT CGGTTACTCT 
GGAGAAGATT ATCCCAGAGC TGCACCCACT GATGTTAAAA TCCGAGTCCT GAACAGCACA 
GCCATCAGCC TTCAGTGGAA CCGCGTCTAC TCCGACACGG TCCAGGGCCA GCTCAGAGAG 
TACCGAGCCT ACTACTGGAG GGAGAGCAGC TTGTTGAAGA ACCTGTGGGT GTCTCAGAAG 
AGGCAGCAAG CAGGCTTCCC CGGCGACCGC CTCCGTGGCG TGGTGTCCCG CCTCTTCCCC 
TACAGTAACT ACAAGCTGGA GATGGCTGTG GTCAATGGGC GAGGCGATGG GCCTCGCAGT 
GAGACCAAGG AGTTCACCAC CCCGGAAGGA GTACCCAGTG CCCCCAGGCG TTTCCGAGTC 
CGGCAGCCCA ACCTGGAGAC AATCAACCTG GAATGGGATC ATCCAGAACA CCCCAATGGG 
ATCCTGACTG GATACACTCT CAAATACGTG GCCTTTAATG GAACCAAAGT AGGAAAGCAG 
ATAGTGGAAA ACTTCTCTCC CAATCAGACC AAGTTCACGA TGCAAAGAGC AGACCCCGTG 
TCGCGCTACC GCTTTTCCCT CAGTGCCAGG ACGCAGGTGG GCTCTGGGGA AGCAGTCACA 
GAGGAGTCAC CAGCACCCCC GAATGAAGCT GCTCCAACCG CAGCTCCTCC CACGTTGCCC 
CCGACCACGG TGGGTGTGAC GGGCACGGTG GGCAGTACTG ATGCCACTGC TGCCACCACC 
GAAGCCACAA CAGTCCCCAC CATCCCAACT GTCGCACCCT CCACCGTCGC CACCACCACC 
GTCGCCACAA CTACTACAAC CACTGCCGCC GCCACCACCA CGGAGAGTCC TCCCACCACC 
ACCAGGACTG AGATTCAAGA ATCCGCCCCC GATGAGCAAT CCATCTGGAA CGTCACAGTG 
CTCCCCAACA GTAAATGGGC CAACATCACC TGGAAGCACA ATTTCGGGCC CGGAACTGAC 
TTTGTGGTTG AGTACATCGA CAGCAACCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG 
GCCCAGCCCA TACAGCTGAC AGACCTCTAT CCCGGTATGA CATACACGTT GCGAGTTTAT 
TCCCGGGACA ACGAGGGCAT CAGCAGTACC GTCATCACCT TTATGACCAG TACAGCCTAC 
ACCAACAACC AGGCGGACAT CGCCACCCAG GGCTGGTTCA TTGGGCTCAT GTGTGCCGTC 
GCCCTCCTGG TGCTGACCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG AGGCGGCAAG 
TACCCAGTGC GAGAAAAGAA GGATGTTCCC CTTGGCCCTG AGGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCTTGC AGGGCAGCCA GACGTCCCTG 
GATGGTACCA TCAAGCAGCA GGAGAGTGAT GACAGCCTGG TGGACTACGG CGAGGGCGGC 
GAGGGACAGT TCAATGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA AAAGGACAAG 
GAAGAGACAG AGGGCAACGA AAGCTCAGAG GCCACATCTC CTGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012393129.1
CDS	1..4029
Translation	MARQQPPPWVHTAFLFCLLSLSGAIEIPMDPSIQNELSQPPTITKQSVKEHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFNLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVYWLKDDEPLYVGNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAILQLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMAVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILTGYTLKYVAFNGTKVGKQIVENFSPNQTKFTMQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAAPTAAPPTLPPTTVGVTGTVGSTDATAATTEATTVPTIPTVAPSTVATTTVATTTTTTAAATTTESPPTTTRTEIQESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAVALLVLTLLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_012537675.1
Organism	Orcinus orca(killer whale)
Definition	Orcinus orca neurofascin (NFASC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012537675.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT GTCCCAGCCC 
CCAACCATCA CCAAGCAGTC AGTGAAGGAG CACATCGTGG ATCCCCGCGA TAACATCTTG 
ATTGAGTGCG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG CAACAGTAGG 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCGATGAGGA GGAGGTCTGG TACCCTGGTG 
ATCGACTTCC GCAGTGGCGG GCGGCCAGAG GAGTATGAGG GGGAATACCA GTGCTTTGCC 
CGCAACAAGT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTCGTTCAAG AAGGTGCTCC CTTGACGCTC 
CAGTGCAACC CCCCACCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCATA ACGGAGACCT GTATTTCTCT 
AATGTGATGC TGCAGGACAT GCAGACGGAC TACAGCTGCA ATGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCATTCAAC CTCAAAGTCC TTACCACCCG AGGAGTTGCA 
GAAAGAACAC CCAGCTTCAT GTATCCCCAG GGCACTGCAA GCAGTCAGAT GGTGCTGCGT 
GGCATGGACC TCCTGCTGGA GTGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCGTCTGAC AAAGCCAAGT TTGAGAACTT TAACAAGGCC 
CTGCGTATCA CCAACGTGTC CGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT CCTGGCTCCT GGCGAGGACG GGAGACTGGT GTGTCGAGCC 
AACGGGAACC CCAAGCCCAC CGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCCAACC CAAACCGTGA GGTGGCCGGG GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CCGTGTACCA GTGCAACACC TCCAACGAGC ACGGCTACCT GCTGGCCAAC 
GCCTTCGTCA GCGTGCTGGA CGTGCCGCCT CGGATGCTGT CACCCCGGAA CCAGCTCATC 
AGGGTGATCC TGTACAACCG GACGCGGCTG GACTGCCCGT TCTTTGGGTC TCCCATCCCC 
ACACTCCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTC 
TACGAGAACG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC 
TGTGTTGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGCAT GCCTGAGGAC CAGGTCGCCA AAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GCGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTGTACTG GCTGAAGGAC 
GACGAGCCCC TCTACGTCGG GAACAGGATG AAGAAGGAAG AGGACTCCCT GACCATCTTT 
GGTGTGGCCG AGCGGGACCA GGGTAGCTAC ACATGCGTTG CCAGCACCGA GCTGGACCAA 
GACCTGGCCA AGGCCTACCT GACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACC TGGAGCTCAC CGACCTGGCC 
GAGAGGAGCG TGAGACTGAC CTGGATTCCG GGGGACGATA ACAACAGCCC CATCACAGAC 
TACGTTGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG 
TTCCCAGGAA GTGTCAACTC AGCCATCCTC CAGCTGTCCC CCTATGTCAA CTACCAGTTC 
CGGGTCATTG CCATCAATGA GGTGGGCAGC AGCCATCCCA GCCTCCCGTC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC TGAGTCCAAC CCCTCTGATG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCG ATGAACGCCA CCTCCGCCTT TGGCCCCAAC 
CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAGACCCGAG AGACCTGGAA CAATGTCACT 
GTGTGGGGCT CCCGCTATGT GGTGGGGCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCAG AAAATGACTT TGGGAAGGGC CCTGAACCAG ACACTGTCAT CGGTTACTCT 
GGAGAAGATT ATCCCAGAGC TGCACCCACT GATGTTAAAA TCCGAGTCCT GAACAGCACA 
GCCATCAGCC TTCAGTGGAA CCGCGTCTAC TCCGACACGG TCCAGGGCCA GCTCAGAGAG 
TACCGAGCCT ACTACTGGAG GGAGAGCAGC TTGTTGAAGA ACCTGTGGGT GTCTCAGAAG 
AGGCAGCAAG CAGGCTTCCC CGGCGACCGC CTCCGTGGCG TGGTGTCCCG CCTCTTCCCC 
TACAGTAACT ACAAGCTGGA GATGGCTGTG GTCAATGGGC GAGGCGATGG GCCTCGCAGT 
GAGACCAAGG AGTTCACCAC CCCGGAAGGA GTACCCAGTG CCCCCAGGCG TTTCCGAGTC 
CGGCAGCCCA ACCTGGAGAC AATCAACCTG GAATGGGATC ATCCAGAACA CCCCAATGGG 
ATCCTGACTG GATACACTCT CAAATACGTG GCCTTTAATG GAACCAAAGT AGGAAAGCAG 
ATAGTGGAAA ACTTCTCTCC CAATCAGACC AAGTTCACGA TGCAAAGAGC AGACCCCGTG 
TCGCGCTACC GCTTTTCCCT CAGTGCCAGG ACGCAGGTGG GCTCTGGGGA AGCAGTCACA 
GAGGAGTCAC CAGCACCCCC GAATGAAGCT GCTCCAACCG CAGCTCCTCC CACGTTGCCC 
CCGACCACGG TGGGTGTGAC GGGCACGGTG GGCAGTACTG ATGCCACTGC TGCCACCACC 
GAAGCCACAA CAGTCCCCAC CATCCCAACT GTCGCACCCT CCACCGTCGC CACCACCACC 
GTCGCCACAA CTACTACAAC CACTGCCGCC GCCACCACCA CGGAGAGTCC TCCCACCACC 
ACCAGGACTG AGATTCAAGA ATCCGCCCCC GATGAGCAAT CCATCTGGAA CGTCACAGTG 
CTCCCCAACA GTAAATGGGC CAACATCACC TGGAAGCACA ATTTCGGGCC CGGAACTGAC 
TTTGTGGTTG AGTACATCGA CAGCAACCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG 
GCCCAGCCCA TACAGCTGAC AGACCTCTAT CCCGGTATGA CATACACGTT GCGAGTTTAT 
TCCCGGGACA ACGAGGGCAT CAGCAGTACC GTCATCACCT TTATGACCAG TACAGCCTAC 
ACCAACAACC AGGCGGACAT CGCCACCCAG GGCTGGTTCA TTGGGCTCAT GTGTGCCGTC 
GCCCTCCTGG TGCTGACCCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG AGGCGGCAAG 
TACCCAGTGC GAGAAAAGAA GGATGTTCCC CTTGGCCCTG AGGACCCCAA AGAAGAGGAT 
GGCTCCTTTG ATTACAGTGA CGAGGACAAC AAGCCCTTGC AGGGCAGCCA GACGTCCCTG 
GATGGTACCA TCAAGCAGCA GGAGAGTGAT GACAGCCTGG TGGACTACGG CGAGGGCGGC 
GAGGGACAGT TCAATGAGGA CGGCTCCTTC ATCGGCCAGT ACACAGTCAA AAAGGACAAG 
GAAGAGACAG AGGGCAACGA AAGCTCAGAG GCCACATCTC CTGTCAACGC CATCTATTCT 
CTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_004282457.1 ) cDNA ORF clone, Orcinus orca(killer whale) -> XP_004282505.1 Orcinus orca neurofascin (NFASC), transcript variant X2, mRNA.

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CloneID	OOr162778
Clone ID Related Accession (Same CDS sequence)	XM_004282457.1
Accession Version	XM_004282457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-14
Organism	Orcinus orca(killer whale)
Product	neurofascin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004438580.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Orcinus orca Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT GTCCCAGCCC 
CCAACCATCA CCAAGCAGTC AGTGAAGGAG CACATCGTGG ATCCCCGCGA TAACATCTTG 
ATTGAGTGCG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG CAACAGTAGG 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCGATGAGGA GGAGGTCTGG TACCCTGGTG 
ATCGACTTCC GCAGTGGCGG GCGGCCAGAG GAGTATGAGG GGGAATACCA GTGCTTTGCC 
CGCAACAAGT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTCGTTCAAG AAGGTGCTCC CTTGACGCTC 
CAGTGCAACC CCCCACCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCATA ACGGAGACCT GTATTTCTCT 
AATGTGATGC TGCAGGACAT GCAGACGGAC TACAGCTGCA ATGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCATTCAAC CTCAAAGTCC TTACCACCCG AGGAGTTGCA 
GAAAGAACAC CCAGCTTCAT GTATCCCCAG GGCACTGCAA GCAGTCAGAT GGTGCTGCGT 
GGCATGGACC TCCTGCTGGA GTGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCGTCTGAC AAAGCCAAGT TTGAGAACTT TAACAAGGCC 
CTGCGTATCA CCAACGTGTC CGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT CCTGGCTCCT GGCGAGGACG GGAGACTGGT GTGTCGAGCC 
AACGGGAACC CCAAGCCCAC CGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCCAACC CAAACCGTGA GGTGGCCGGG GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CCGTGTACCA GTGCAACACC TCCAACGAGC ACGGCTACCT GCTGGCCAAC 
GCCTTCGTCA GCGTGCTGGA CGTGCCGCCT CGGATGCTGT CACCCCGGAA CCAGCTCATC 
AGGGTGATCC TGTACAACCG GACGCGGCTG GACTGCCCGT TCTTTGGGTC TCCCATCCCC 
ACACTCCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTC 
TACGAGAACG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC 
TGTGTTGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGCAT GCCTGAGGAC CAGGTCGCCA AAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GCGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTGTACTG GCTGAAGGAC 
GACGAGCCCC TCTACGTCGG GAACAGGATG AAGAAGGAAG AGGACTCCCT GACCATCTTT 
GGTGTGGCCG AGCGGGACCA GGGTAGCTAC ACATGCGTTG CCAGCACCGA GCTGGACCAA 
GACCTGGCCA AGGCCTACCT GACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACC TGGAGCTCAC CGACCTGGCC 
GAGAGGAGCG TGAGACTGAC CTGGATTCCG GGGGACGATA ACAACAGCCC CATCACAGAC 
TACGTTGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG 
TTCCCAGGAA GTGTCAACTC AGCCATCCTC CAGCTGTCCC CCTATGTCAA CTACCAGTTC 
CGGGTCATTG CCATCAATGA GGTGGGCAGC AGCCATCCCA GCCTCCCGTC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC TGAGTCCAAC CCCTCTGATG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCG ATGAACGCCA CCTCCGCCTT TGGCCCCAAC 
CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAGACCCGAG AGACCTGGAA CAATGTCACT 
GTGTGGGGCT CCCGCTATGT GGTGGGGCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCAG AAAATGACTT TGGGAAGGGC CCTGAACCAG ACACTGTCAT CGGTTACTCT 
GGAGAAGATT TACCCAGTGC CCCCAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCAGAACAC CCCAATGGGA TCCTGACTGG ATACACTCTC 
AAATACGTGG CCTTTAATGG AACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGAT GCAAAGAGCA GACCCCGTGT CGCGCTACCG CTTTTCCCTC 
AGTGCCAGGA CGCAGGTGGG CTCTGGGGAA GCAGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTG CTCCAACCGC AGCTCCTCCC ACGTTGCCCC CGACCACGGT GGGTGTGACG 
GGCACGGTGG GCAGTACTGA TGCCACTGCT GCCACCACCG AAGCCACAAC AGTCCCCACC 
ATCCCAACTG TCGCACCCTC CACCGTCGCC ACCACCACCG TCGCCACAAC TACTACAACC 
ACTGCCGCCG CCACCACCAC GGAGAGTCCT CCCACCACCA CCAGGACTGA GATTCAAGAA 
TCCGCCCCCG ATGAGCAATC CATCTGGAAC GTCACAGTGC TCCCCAACAG TAAATGGGCC 
AACATCACCT GGAAGCACAA TTTCGGGCCC GGAACTGACT TTGTGGTTGA GTACATCGAC 
AGCAACCATA CGAAAAAAAC TGTCCCTGTT AAGGCCCAGG CCCAGCCCAT ACAGCTGACA 
GACCTCTATC CCGGTATGAC ATACACGTTG CGAGTTTATT CCCGGGACAA CGAGGGCATC 
AGCAGTACCG TCATCACCTT TATGACCAGT ACAGCCTACA CCAACAACCA GGCGGACATC 
GCCACCCAGG GCTGGTTCAT TGGGCTCATG TGTGCCGTCG CCCTCCTGGT GCTGACCCTG 
CTCATCGTCT GTTTCATCAA GAGGAGTCGA GGCGGCAAGT ACCCAGTGCG AGAAAAGAAG 
GATGTTCCCC TTGGCCCTGA GGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC 
GAGGACAACA AGCCCTTGCA GGGCAGCCAG ACGTCCCTGG ATGGTACCAT CAAGCAGCAG 
GAGAGTGATG ACAGCCTGGT GGACTACGGC GAGGGCGGCG AGGGACAGTT CAATGAGGAC 
GGCTCCTTCA TCGGCCAGTA CACAGTCAAA AAGGACAAGG AAGAGACAGA GGGCAACGAA 
AGCTCAGAGG CCACATCTCC TGTCAACGCC ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004282505.1
CDS	319..4026
Translation	MARQQPPPWVHTAFLFCLLSLSGAIEIPMDPSIQNELSQPPTITKQSVKEHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFNLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVYWLKDDEPLYVGNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAILQLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILTGYTLKYVAFNGTKVGKQIVENFSPNQTKFTMQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAAPTAAPPTLPPTTVGVTGTVGSTDATAATTEATTVPTIPTVAPSTVATTTVATTTTTTAAATTTESPPTTTRTEIQESAPDEQSIWNVTVLPNSKWANITWKHNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAVALLVLTLLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_004282457.1
Organism	Orcinus orca(killer whale)
Definition	Orcinus orca neurofascin (NFASC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004282457.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT GTCCCAGCCC 
CCAACCATCA CCAAGCAGTC AGTGAAGGAG CACATCGTGG ATCCCCGCGA TAACATCTTG 
ATTGAGTGCG AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG CAACAGTAGG 
TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCGATGAGGA GGAGGTCTGG TACCCTGGTG 
ATCGACTTCC GCAGTGGCGG GCGGCCAGAG GAGTATGAGG GGGAATACCA GTGCTTTGCC 
CGCAACAAGT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAATCTCCT 
CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTCGTTCAAG AAGGTGCTCC CTTGACGCTC 
CAGTGCAACC CCCCACCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCATA ACGGAGACCT GTATTTCTCT 
AATGTGATGC TGCAGGACAT GCAGACGGAC TACAGCTGCA ATGCCCGCTT CCACTTCACC 
CACACCATCC AGCAGAAGAA CCCATTCAAC CTCAAAGTCC TTACCACCCG AGGAGTTGCA 
GAAAGAACAC CCAGCTTCAT GTATCCCCAG GGCACTGCAA GCAGTCAGAT GGTGCTGCGT 
GGCATGGACC TCCTGCTGGA GTGCATCGCC TCCGGGGTCC CAACACCAGA CATCGCATGG 
TACAAGAAAG GTGGGGACCT CCCGTCTGAC AAAGCCAAGT TTGAGAACTT TAACAAGGCC 
CTGCGTATCA CCAACGTGTC CGAGGAAGAC TCCGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG 
GACGAACCCA AGAACCTTAT CCTGGCTCCT GGCGAGGACG GGAGACTGGT GTGTCGAGCC 
AACGGGAACC CCAAGCCCAC CGTCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA 
CCACCCAACC CAAACCGTGA GGTGGCCGGG GACACCATCA TCTTCCGGGA CACCCAGATC 
AGCAGCAGGG CCGTGTACCA GTGCAACACC TCCAACGAGC ACGGCTACCT GCTGGCCAAC 
GCCTTCGTCA GCGTGCTGGA CGTGCCGCCT CGGATGCTGT CACCCCGGAA CCAGCTCATC 
AGGGTGATCC TGTACAACCG GACGCGGCTG GACTGCCCGT TCTTTGGGTC TCCCATCCCC 
ACACTCCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGCAA CTACCATGTC 
TACGAGAACG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC 
TGTGTTGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACCGCAT GCCTGAGGAC CAGGTCGCCA AAAGGGGCAC CACGGTGCAG 
CTGGAGTGTC GCGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTGTACTG GCTGAAGGAC 
GACGAGCCCC TCTACGTCGG GAACAGGATG AAGAAGGAAG AGGACTCCCT GACCATCTTT 
GGTGTGGCCG AGCGGGACCA GGGTAGCTAC ACATGCGTTG CCAGCACCGA GCTGGACCAA 
GACCTGGCCA AGGCCTACCT GACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACC TGGAGCTCAC CGACCTGGCC 
GAGAGGAGCG TGAGACTGAC CTGGATTCCG GGGGACGATA ACAACAGCCC CATCACAGAC 
TACGTTGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG 
TTCCCAGGAA GTGTCAACTC AGCCATCCTC CAGCTGTCCC CCTATGTCAA CTACCAGTTC 
CGGGTCATTG CCATCAATGA GGTGGGCAGC AGCCATCCCA GCCTCCCGTC CGAGCGCTAC 
CGAACCAGTG GAGCACCCCC TGAGTCCAAC CCCTCTGATG TGAAGGGAGA GGGGACCAGA 
AAGAACAACA TGGAGATCAC GTGGACGCCG ATGAACGCCA CCTCCGCCTT TGGCCCCAAC 
CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAGACCCGAG AGACCTGGAA CAATGTCACT 
GTGTGGGGCT CCCGCTATGT GGTGGGGCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA 
GTCCAGGCAG AAAATGACTT TGGGAAGGGC CCTGAACCAG ACACTGTCAT CGGTTACTCT 
GGAGAAGATT TACCCAGTGC CCCCAGGCGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA 
ATCAACCTGG AATGGGATCA TCCAGAACAC CCCAATGGGA TCCTGACTGG ATACACTCTC 
AAATACGTGG CCTTTAATGG AACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCACGAT GCAAAGAGCA GACCCCGTGT CGCGCTACCG CTTTTCCCTC 
AGTGCCAGGA CGCAGGTGGG CTCTGGGGAA GCAGTCACAG AGGAGTCACC AGCACCCCCG 
AATGAAGCTG CTCCAACCGC AGCTCCTCCC ACGTTGCCCC CGACCACGGT GGGTGTGACG 
GGCACGGTGG GCAGTACTGA TGCCACTGCT GCCACCACCG AAGCCACAAC AGTCCCCACC 
ATCCCAACTG TCGCACCCTC CACCGTCGCC ACCACCACCG TCGCCACAAC TACTACAACC 
ACTGCCGCCG CCACCACCAC GGAGAGTCCT CCCACCACCA CCAGGACTGA GATTCAAGAA 
TCCGCCCCCG ATGAGCAATC CATCTGGAAC GTCACAGTGC TCCCCAACAG TAAATGGGCC 
AACATCACCT GGAAGCACAA TTTCGGGCCC GGAACTGACT TTGTGGTTGA GTACATCGAC 
AGCAACCATA CGAAAAAAAC TGTCCCTGTT AAGGCCCAGG CCCAGCCCAT ACAGCTGACA 
GACCTCTATC CCGGTATGAC ATACACGTTG CGAGTTTATT CCCGGGACAA CGAGGGCATC 
AGCAGTACCG TCATCACCTT TATGACCAGT ACAGCCTACA CCAACAACCA GGCGGACATC 
GCCACCCAGG GCTGGTTCAT TGGGCTCATG TGTGCCGTCG CCCTCCTGGT GCTGACCCTG 
CTCATCGTCT GTTTCATCAA GAGGAGTCGA GGCGGCAAGT ACCCAGTGCG AGAAAAGAAG 
GATGTTCCCC TTGGCCCTGA GGACCCCAAA GAAGAGGATG GCTCCTTTGA TTACAGTGAC 
GAGGACAACA AGCCCTTGCA GGGCAGCCAG ACGTCCCTGG ATGGTACCAT CAAGCAGCAG 
GAGAGTGATG ACAGCCTGGT GGACTACGGC GAGGGCGGCG AGGGACAGTT CAATGAGGAC 
GGCTCCTTCA TCGGCCAGTA CACAGTCAAA AAGGACAAGG AAGAGACAGA GGGCAACGAA 
AGCTCAGAGG CCACATCTCC TGTCAACGCC ATCTATTCTC TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_004282458.1 ) cDNA ORF clone, Orcinus orca(killer whale) -> XP_004282506.1 Orcinus orca neurofascin (NFASC), transcript variant X3, mRNA.

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CloneID	OOr162779
Clone ID Related Accession (Same CDS sequence)	XM_004282458.1
Accession Version	XM_004282458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-14
Organism	Orcinus orca(killer whale)
Product	neurofascin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004438580.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Orcinus orca Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CTGTCCCAGC CCCCAACCAT CACCAAGCAG 
TCAGTGAAGG AGCACATCGT GGATCCCCGC GATAACATCT TGATTGAGTG CGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACT CGCAACAGTA GGTTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCGATGAG GAGGAGGTCT GGTACCCTGG TGATCGACTT CCGCAGTGGC 
GGGCGGCCAG AGGAGTATGA GGGGGAATAC CAGTGCTTTG CCCGCAACAA GTTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTCGTTCA AGAAGGTGCT CCCTTGACGC TCCAGTGCAA CCCCCCACCT 
GGACTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAGCGTGTCT CCCAGGGCCA TAACGGAGAC CTGTATTTCT CTAATGTGAT GCTGCAGGAC 
ATGCAGACGG ACTACAGCTG CAATGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCATTCA ACCTCAAAGT CCTTACCAAC CACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCCAGCTT CATGTATCCC 
CAGGGCACTG CAAGCAGTCA GATGGTGCTG CGTGGCATGG ACCTCCTGCT GGAGTGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCGTCT 
GACAAAGCCA AGTTTGAGAA CTTTAACAAG GCCCTGCGTA TCACCAACGT GTCCGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT 
CCTGGCGAGG ACGGGAGACT GGTGTGTCGA GCCAACGGGA ACCCCAAGCC CACCGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCC 
GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCCGTGTA CCAGTGCAAC 
ACCTCCAACG AGCACGGCTA CCTGCTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCG 
CCTCGGATGC TGTCACCCCG GAACCAGCTC ATCAGGGTGA TCCTGTACAA CCGGACGCGG 
CTGGACTGCC CGTTCTTTGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTCTACGAGA ACGGCAGTCT GGAAATCAAG 
ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTTG CCACCAACAT CCTGGGCAAA 
GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG CATGCCTGAG 
GACCAGGTCG CCAAAAGGGG CACCACGGTG CAGCTGGAGT GTCGCGTGAA GCACGACCCC 
TCCCTGAAGC TCACGGTGTA CTGGCTGAAG GACGACGAGC CCCTCTACGT CGGGAACAGG 
ATGAAGAAGG AAGAGGACTC CCTGACCATC TTTGGTGTGG CCGAGCGGGA CCAGGGTAGC 
TACACATGCG TTGCCAGCAC CGAGCTGGAC CAAGACCTGG CCAAGGCCTA CCTGACCGTG 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC 
CGACCCCGGG ACCTGGAGCT CACCGACCTG GCCGAGAGGA GCGTGAGACT GACCTGGATT 
CCGGGGGACG ATAACAACAG CCCCATCACA GACTACGTTG TCCAGTTTGA AGAGGACCAG 
TTCCAGCCAG GGGTCTGGCA TGACCATTCC AAGTTCCCAG GAAGTGTCAA CTCAGCCATC 
CTCCAGCTGT CCCCCTATGT CAACTACCAG TTCCGGGTCA TTGCCATCAA TGAGGTGGGC 
AGCAGCCATC CCAGCCTCCC GTCCGAGCGC TACCGAACCA GTGGAGCACC CCCTGAGTCC 
AACCCCTCTG ATGTGAAGGG AGAGGGGACC AGAAAGAACA ACATGGAGAT CACGTGGACG 
CCGATGAACG CCACCTCCGC CTTTGGCCCC AACCTGCGCT ACATCGTCAA GTGGCGGCGG 
AGAGAGACCC GAGAGACCTG GAACAATGTC ACTGTGTGGG GCTCCCGCTA TGTGGTGGGG 
CAGACCCCTG TCTACGTGCC CTATGAGATC CGAGTCCAGG CAGAAAATGA CTTTGGGAAG 
GGCCCTGAAC CAGACACTGT CATCGGTTAC TCTGGAGAAG ATTATCCCAG AGCTGCACCC 
ACTGATGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC 
TACTCCGACA CGGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC 
AGCTTGTTGA AGAACCTGTG GGTGTCTCAG AAGAGGCAGC AAGCAGGCTT CCCCGGCGAC 
CGCCTCCGTG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGCT 
GTGGTCAATG GGCGAGGCGA TGGGCCTCGC AGTGAGACCA AGGAGTTCAC CACCCCGGAA 
GGAGTACCCA GTGCCCCCAG GCGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC 
CTGGAATGGG ATCATCCAGA ACACCCCAAT GGGATCCTGA CTGGATACAC TCTCAAATAC 
GTGGCCTTTA ATGGAACCAA AGTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCA CGATGCAAAG AGCAGACCCC GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC 
AGGACGCAGG TGGGCTCTGG GGAAGCAGTC ACAGAGGAGT CACCAGCACC CCCGAATGAA 
GCTGCTCCAA CCGCAGCCTA CACCAACAAC CAGGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTCA TGTGTGCCGT CGCCCTCCTG GTGCTGACCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GAGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAGGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCTTG 
CAGGGCAGCC AGACGTCCCT GGATGGTACC ATCAAGCAGC AGGAGAGTGA TGACAGCCTG 
GTGGACTACG GCGAGGGCGG CGAGGGACAG TTCAATGAGG ACGGCTCCTT CATCGGCCAG 
TACACAGTCA AAAAGGACAA GGAAGAGACA GAGGGCAACG AAAGCTCAGA GGCCACATCT 
CCTGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004282506.1
CDS	75..3644
Translation	MARQQPPPWVHTAFLFCLLSLSGAIEIPMDLSQPPTITKQSVKEHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFNLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVYWLKDDEPLYVGNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAILQLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMAVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILTGYTLKYVAFNGTKVGKQIVENFSPNQTKFTMQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAAPTAAYTNNQADIATQGWFIGLMCAVALLVLTLLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_004282458.1
Organism	Orcinus orca(killer whale)
Definition	Orcinus orca neurofascin (NFASC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004282458.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CTGTCCCAGC CCCCAACCAT CACCAAGCAG 
TCAGTGAAGG AGCACATCGT GGATCCCCGC GATAACATCT TGATTGAGTG CGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACT CGCAACAGTA GGTTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCGATGAG GAGGAGGTCT GGTACCCTGG TGATCGACTT CCGCAGTGGC 
GGGCGGCCAG AGGAGTATGA GGGGGAATAC CAGTGCTTTG CCCGCAACAA GTTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTCGTTCA AGAAGGTGCT CCCTTGACGC TCCAGTGCAA CCCCCCACCT 
GGACTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAGCGTGTCT CCCAGGGCCA TAACGGAGAC CTGTATTTCT CTAATGTGAT GCTGCAGGAC 
ATGCAGACGG ACTACAGCTG CAATGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCATTCA ACCTCAAAGT CCTTACCAAC CACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCCAGCTT CATGTATCCC 
CAGGGCACTG CAAGCAGTCA GATGGTGCTG CGTGGCATGG ACCTCCTGCT GGAGTGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCGTCT 
GACAAAGCCA AGTTTGAGAA CTTTAACAAG GCCCTGCGTA TCACCAACGT GTCCGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT 
CCTGGCGAGG ACGGGAGACT GGTGTGTCGA GCCAACGGGA ACCCCAAGCC CACCGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCC 
GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCCGTGTA CCAGTGCAAC 
ACCTCCAACG AGCACGGCTA CCTGCTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCG 
CCTCGGATGC TGTCACCCCG GAACCAGCTC ATCAGGGTGA TCCTGTACAA CCGGACGCGG 
CTGGACTGCC CGTTCTTTGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTCTACGAGA ACGGCAGTCT GGAAATCAAG 
ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTTG CCACCAACAT CCTGGGCAAA 
GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG CATGCCTGAG 
GACCAGGTCG CCAAAAGGGG CACCACGGTG CAGCTGGAGT GTCGCGTGAA GCACGACCCC 
TCCCTGAAGC TCACGGTGTA CTGGCTGAAG GACGACGAGC CCCTCTACGT CGGGAACAGG 
ATGAAGAAGG AAGAGGACTC CCTGACCATC TTTGGTGTGG CCGAGCGGGA CCAGGGTAGC 
TACACATGCG TTGCCAGCAC CGAGCTGGAC CAAGACCTGG CCAAGGCCTA CCTGACCGTG 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC 
CGACCCCGGG ACCTGGAGCT CACCGACCTG GCCGAGAGGA GCGTGAGACT GACCTGGATT 
CCGGGGGACG ATAACAACAG CCCCATCACA GACTACGTTG TCCAGTTTGA AGAGGACCAG 
TTCCAGCCAG GGGTCTGGCA TGACCATTCC AAGTTCCCAG GAAGTGTCAA CTCAGCCATC 
CTCCAGCTGT CCCCCTATGT CAACTACCAG TTCCGGGTCA TTGCCATCAA TGAGGTGGGC 
AGCAGCCATC CCAGCCTCCC GTCCGAGCGC TACCGAACCA GTGGAGCACC CCCTGAGTCC 
AACCCCTCTG ATGTGAAGGG AGAGGGGACC AGAAAGAACA ACATGGAGAT CACGTGGACG 
CCGATGAACG CCACCTCCGC CTTTGGCCCC AACCTGCGCT ACATCGTCAA GTGGCGGCGG 
AGAGAGACCC GAGAGACCTG GAACAATGTC ACTGTGTGGG GCTCCCGCTA TGTGGTGGGG 
CAGACCCCTG TCTACGTGCC CTATGAGATC CGAGTCCAGG CAGAAAATGA CTTTGGGAAG 
GGCCCTGAAC CAGACACTGT CATCGGTTAC TCTGGAGAAG ATTATCCCAG AGCTGCACCC 
ACTGATGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC 
TACTCCGACA CGGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC 
AGCTTGTTGA AGAACCTGTG GGTGTCTCAG AAGAGGCAGC AAGCAGGCTT CCCCGGCGAC 
CGCCTCCGTG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGCT 
GTGGTCAATG GGCGAGGCGA TGGGCCTCGC AGTGAGACCA AGGAGTTCAC CACCCCGGAA 
GGAGTACCCA GTGCCCCCAG GCGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC 
CTGGAATGGG ATCATCCAGA ACACCCCAAT GGGATCCTGA CTGGATACAC TCTCAAATAC 
GTGGCCTTTA ATGGAACCAA AGTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCA CGATGCAAAG AGCAGACCCC GTGTCGCGCT ACCGCTTTTC CCTCAGTGCC 
AGGACGCAGG TGGGCTCTGG GGAAGCAGTC ACAGAGGAGT CACCAGCACC CCCGAATGAA 
GCTGCTCCAA CCGCAGCCTA CACCAACAAC CAGGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTCA TGTGTGCCGT CGCCCTCCTG GTGCTGACCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GAGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAGGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCTTG 
CAGGGCAGCC AGACGTCCCT GGATGGTACC ATCAAGCAGC AGGAGAGTGA TGACAGCCTG 
GTGGACTACG GCGAGGGCGG CGAGGGACAG TTCAATGAGG ACGGCTCCTT CATCGGCCAG 
TACACAGTCA AAAAGGACAA GGAAGAGACA GAGGGCAACG AAAGCTCAGA GGCCACATCT 
CCTGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_004282459.1 ) cDNA ORF clone, Orcinus orca(killer whale) -> XP_004282507.1 Orcinus orca neurofascin (NFASC), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOr162780
Clone ID Related Accession (Same CDS sequence)	XM_004282459.1
Accession Version	XM_004282459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-14
Organism	Orcinus orca(killer whale)
Product	neurofascin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004438580.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Orcinus orca Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CTGTCCCAGC CCCCAACCAT CACCAAGCAG 
TCAGTGAAGG AGCACATCGT GGATCCCCGC GATAACATCT TGATTGAGTG CGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACT CGCAACAGTA GGTTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCGATGAG GAGGAGGTCT GGTACCCTGG TGATCGACTT CCGCAGTGGC 
GGGCGGCCAG AGGAGTATGA GGGGGAATAC CAGTGCTTTG CCCGCAACAA GTTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTCGTTCA AGAAGGTGCT CCCTTGACGC TCCAGTGCAA CCCCCCACCT 
GGACTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAGCGTGTCT CCCAGGGCCA TAACGGAGAC CTGTATTTCT CTAATGTGAT GCTGCAGGAC 
ATGCAGACGG ACTACAGCTG CAATGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCATTCA ACCTCAAAGT CCTTACCAAC CACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCCAGCTT CATGTATCCC 
CAGGGCACTG CAAGCAGTCA GATGGTGCTG CGTGGCATGG ACCTCCTGCT GGAGTGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCGTCT 
GACAAAGCCA AGTTTGAGAA CTTTAACAAG GCCCTGCGTA TCACCAACGT GTCCGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT 
CCTGGCGAGG ACGGGAGACT GGTGTGTCGA GCCAACGGGA ACCCCAAGCC CACCGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCC 
GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCCGTGTA CCAGTGCAAC 
ACCTCCAACG AGCACGGCTA CCTGCTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCG 
CCTCGGATGC TGTCACCCCG GAACCAGCTC ATCAGGGTGA TCCTGTACAA CCGGACGCGG 
CTGGACTGCC CGTTCTTTGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTCTACGAGA ACGGCAGTCT GGAAATCAAG 
ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTTG CCACCAACAT CCTGGGCAAA 
GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG CATGCCTGAG 
GACCAGGTCG CCAAAAGGGG CACCACGGTG CAGCTGGAGT GTCGCGTGAA GCACGACCCC 
TCCCTGAAGC TCACGGTGTA CTGGCTGAAG GACGACGAGC CCCTCTACGT CGGGAACAGG 
ATGAAGAAGG AAGAGGACTC CCTGACCATC TTTGGTGTGG CCGAGCGGGA CCAGGGTAGC 
TACACATGCG TTGCCAGCAC CGAGCTGGAC CAAGACCTGG CCAAGGCCTA CCTGACCGTG 
CTAGGACGGC CAGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCCGA GAGGAGCGTG 
AGACTGACCT GGATTCCGGG GGACGATAAC AACAGCCCCA TCACAGACTA CGTTGTCCAG 
TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGAAGT 
GTCAACTCAG CCATCCTCCA GCTGTCCCCC TATGTCAACT ACCAGTTCCG GGTCATTGCC 
ATCAATGAGG TGGGCAGCAG CCATCCCAGC CTCCCGTCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCTG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCGAT GAACGCCACC TCCGCCTTTG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGAGAG ACCTGGAACA ATGTCACTGT GTGGGGCTCC 
CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCAGAA 
AATGACTTTG GGAAGGGCCC TGAACCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGAGCTG CACCCACTGA TGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GTTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCA 
GGCTTCCCCG GCGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGCTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGACTGGA 
TACACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGATG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CAGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCTGC TCCAACCGCA GCCTACACCA ACAACCAGGC GGACATCGCC 
ACCCAGGGCT GGTTCATTGG GCTCATGTGT GCCGTCGCCC TCCTGGTGCT GACCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGAGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT 
GTTCCCCTTG GCCCTGAGGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCTTGCAGGG CAGCCAGACG TCCCTGGATG GTACCATCAA GCAGCAGGAG 
AGTGATGACA GCCTGGTGGA CTACGGCGAG GGCGGCGAGG GACAGTTCAA TGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAAAAG GACAAGGAAG AGACAGAGGG CAACGAAAGC 
TCAGAGGCCA CATCTCCTGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004282507.1
CDS	319..3843
Translation	MARQQPPPWVHTAFLFCLLSLSGAIEIPMDLSQPPTITKQSVKEHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFNLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVYWLKDDEPLYVGNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAILQLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMAVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILTGYTLKYVAFNGTKVGKQIVENFSPNQTKFTMQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAAPTAAYTNNQADIATQGWFIGLMCAVALLVLTLLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_004282459.1
Organism	Orcinus orca(killer whale)
Definition	Orcinus orca neurofascin (NFASC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004282459.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CTGTCCCAGC CCCCAACCAT CACCAAGCAG 
TCAGTGAAGG AGCACATCGT GGATCCCCGC GATAACATCT TGATTGAGTG CGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACT CGCAACAGTA GGTTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCGATGAG GAGGAGGTCT GGTACCCTGG TGATCGACTT CCGCAGTGGC 
GGGCGGCCAG AGGAGTATGA GGGGGAATAC CAGTGCTTTG CCCGCAACAA GTTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTCGTTCA AGAAGGTGCT CCCTTGACGC TCCAGTGCAA CCCCCCACCT 
GGACTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAGCGTGTCT CCCAGGGCCA TAACGGAGAC CTGTATTTCT CTAATGTGAT GCTGCAGGAC 
ATGCAGACGG ACTACAGCTG CAATGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCATTCA ACCTCAAAGT CCTTACCAAC CACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCCAGCTT CATGTATCCC 
CAGGGCACTG CAAGCAGTCA GATGGTGCTG CGTGGCATGG ACCTCCTGCT GGAGTGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCGTCT 
GACAAAGCCA AGTTTGAGAA CTTTAACAAG GCCCTGCGTA TCACCAACGT GTCCGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT 
CCTGGCGAGG ACGGGAGACT GGTGTGTCGA GCCAACGGGA ACCCCAAGCC CACCGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCC 
GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCCGTGTA CCAGTGCAAC 
ACCTCCAACG AGCACGGCTA CCTGCTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCG 
CCTCGGATGC TGTCACCCCG GAACCAGCTC ATCAGGGTGA TCCTGTACAA CCGGACGCGG 
CTGGACTGCC CGTTCTTTGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTCTACGAGA ACGGCAGTCT GGAAATCAAG 
ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTTG CCACCAACAT CCTGGGCAAA 
GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG CATGCCTGAG 
GACCAGGTCG CCAAAAGGGG CACCACGGTG CAGCTGGAGT GTCGCGTGAA GCACGACCCC 
TCCCTGAAGC TCACGGTGTA CTGGCTGAAG GACGACGAGC CCCTCTACGT CGGGAACAGG 
ATGAAGAAGG AAGAGGACTC CCTGACCATC TTTGGTGTGG CCGAGCGGGA CCAGGGTAGC 
TACACATGCG TTGCCAGCAC CGAGCTGGAC CAAGACCTGG CCAAGGCCTA CCTGACCGTG 
CTAGGACGGC CAGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCCGA GAGGAGCGTG 
AGACTGACCT GGATTCCGGG GGACGATAAC AACAGCCCCA TCACAGACTA CGTTGTCCAG 
TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGAAGT 
GTCAACTCAG CCATCCTCCA GCTGTCCCCC TATGTCAACT ACCAGTTCCG GGTCATTGCC 
ATCAATGAGG TGGGCAGCAG CCATCCCAGC CTCCCGTCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCTG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCGAT GAACGCCACC TCCGCCTTTG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGAGAG ACCTGGAACA ATGTCACTGT GTGGGGCTCC 
CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCAGAA 
AATGACTTTG GGAAGGGCCC TGAACCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGAGCTG CACCCACTGA TGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GTTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCA 
GGCTTCCCCG GCGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGCTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGACTGGA 
TACACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGATG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CAGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCTGC TCCAACCGCA GCCTACACCA ACAACCAGGC GGACATCGCC 
ACCCAGGGCT GGTTCATTGG GCTCATGTGT GCCGTCGCCC TCCTGGTGCT GACCCTGCTC 
ATCGTCTGTT TCATCAAGAG GAGTCGAGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT 
GTTCCCCTTG GCCCTGAGGA CCCCAAAGAA GAGGATGGCT CCTTTGATTA CAGTGACGAG 
GACAACAAGC CCTTGCAGGG CAGCCAGACG TCCCTGGATG GTACCATCAA GCAGCAGGAG 
AGTGATGACA GCCTGGTGGA CTACGGCGAG GGCGGCGAGG GACAGTTCAA TGAGGACGGC 
TCCTTCATCG GCCAGTACAC AGTCAAAAAG GACAAGGAAG AGACAGAGGG CAACGAAAGC 
TCAGAGGCCA CATCTCCTGT CAACGCCATC TATTCTCTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_004282460.2 ) cDNA ORF clone, Orcinus orca(killer whale) -> XP_004282508.1 Orcinus orca neurofascin (NFASC), transcript variant X5, mRNA.

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CloneID	OOr162781
Clone ID Related Accession (Same CDS sequence)	XM_004282460.2
Accession Version	XM_004282460.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-14
Organism	Orcinus orca(killer whale)
Product	neurofascin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004438580.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_004282460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Orcinus orca Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CTGTCCCAGC CCCCAACCAT CACCAAGCAG 
TCAGTGAAGG AGCACATCGT GGATCCCCGC GATAACATCT TGATTGAGTG CGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACT CGCAACAGTA GGTTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCGATGAG GAGGAGGTCT GGTACCCTGG TGATCGACTT CCGCAGTGGC 
GGGCGGCCAG AGGAGTATGA GGGGGAATAC CAGTGCTTTG CCCGCAACAA GTTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTCGTTCA AGAAGGTGCT CCCTTGACGC TCCAGTGCAA CCCCCCACCT 
GGACTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAGCGTGTCT CCCAGGGCCA TAACGGAGAC CTGTATTTCT CTAATGTGAT GCTGCAGGAC 
ATGCAGACGG ACTACAGCTG CAATGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCATTCA ACCTCAAAGT CCTTACCAAC CACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCCAGCTT CATGTATCCC 
CAGGGCACTG CAAGCAGTCA GATGGTGCTG CGTGGCATGG ACCTCCTGCT GGAGTGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCGTCT 
GACAAAGCCA AGTTTGAGAA CTTTAACAAG GCCCTGCGTA TCACCAACGT GTCCGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT 
CCTGGCGAGG ACGGGAGACT GGTGTGTCGA GCCAACGGGA ACCCCAAGCC CACCGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCC 
GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCCGTGTA CCAGTGCAAC 
ACCTCCAACG AGCACGGCTA CCTGCTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCG 
CCTCGGATGC TGTCACCCCG GAACCAGCTC ATCAGGGTGA TCCTGTACAA CCGGACGCGG 
CTGGACTGCC CGTTCTTTGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTCTACGAGA ACGGCAGTCT GGAAATCAAG 
ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTTG CCACCAACAT CCTGGGCAAA 
GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG CATGCCTGAG 
GACCAGGTCG CCAAAAGGGG CACCACGGTG CAGCTGGAGT GTCGCGTGAA GCACGACCCC 
TCCCTGAAGC TCACGGTGTA CTGGCTGAAG GACGACGAGC CCCTCTACGT CGGGAACAGG 
ATGAAGAAGG AAGAGGACTC CCTGACCATC TTTGGTGTGG CCGAGCGGGA CCAGGGTAGC 
TACACATGCG TTGCCAGCAC CGAGCTGGAC CAAGACCTGG CCAAGGCCTA CCTGACCGTG 
CTAGGACGGC CAGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCCGA GAGGAGCGTG 
AGACTGACCT GGATTCCGGG GGACGATAAC AACAGCCCCA TCACAGACTA CGTTGTCCAG 
TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGAAGT 
GTCAACTCAG CCATCCTCCA GCTGTCCCCC TATGTCAACT ACCAGTTCCG GGTCATTGCC 
ATCAATGAGG TGGGCAGCAG CCATCCCAGC CTCCCGTCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCTG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCGAT GAACGCCACC TCCGCCTTTG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGAGAG ACCTGGAACA ATGTCACTGT GTGGGGCTCC 
CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCAGAA 
AATGACTTTG GGAAGGGCCC TGAACCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGAGCTG CACCCACTGA TGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GTTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCA 
GGCTTCCCCG GCGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGCTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGACTGGA 
TACACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGATG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CAGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCCTA CACCAACAAC CAGGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTCA TGTGTGCCGT CGCCCTCCTG GTGCTGACCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GAGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAGGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCTTG 
CAGGGCAGCC AGACGTCCCT GGATGGTACC ATCAAGCAGC AGGAGAGTGA TGACAGCCTG 
GTGGACTACG GCGAGGGCGG CGAGGGACAG TTCAATGAGG ACGGCTCCTT CATCGGCCAG 
TACACAGTCA AAAAGGACAA GGAAGAGACA GAGGGCAACG AAAGCTCAGA GGCCACATCT 
CCTGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004282508.1
CDS	319..3828
Translation	MARQQPPPWVHTAFLFCLLSLSGAIEIPMDLSQPPTITKQSVKEHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFNLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVYWLKDDEPLYVGNRMKKEEDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAILQLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTVIGYSGEDYPRAAPTDVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQAGFPGDRLRGVVSRLFPYSNYKLEMAVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILTGYTLKYVAFNGTKVGKQIVENFSPNQTKFTMQRADPVSRYRFSLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAVALLVLTLLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_004282460.2
Organism	Orcinus orca(killer whale)
Definition	Orcinus orca neurofascin (NFASC), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004282460.2

ORF Insert Sequence:

ATGGCCAGGC AGCAGCCACC GCCCTGGGTC CACACAGCCT TCCTCTTCTG CCTGCTCAGC 
CTCAGCGGGG CCATCGAGAT TCCCATGGAT CTGTCCCAGC CCCCAACCAT CACCAAGCAG 
TCAGTGAAGG AGCACATCGT GGATCCCCGC GATAACATCT TGATTGAGTG CGAAGCAAAA 
GGGAACCCTG CCCCCAGCTT CCACTGGACT CGCAACAGTA GGTTCTTCAA CATCGCCAAG 
GACCCCCGGG TGTCGATGAG GAGGAGGTCT GGTACCCTGG TGATCGACTT CCGCAGTGGC 
GGGCGGCCAG AGGAGTATGA GGGGGAATAC CAGTGCTTTG CCCGCAACAA GTTTGGCACG 
GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAATCTC CTCTGTGGCC CAAGGAAAAC 
CTAGACCCTG TCGTCGTTCA AGAAGGTGCT CCCTTGACGC TCCAGTGCAA CCCCCCACCT 
GGACTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAAGAC 
AAGCGTGTCT CCCAGGGCCA TAACGGAGAC CTGTATTTCT CTAATGTGAT GCTGCAGGAC 
ATGCAGACGG ACTACAGCTG CAATGCCCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG 
AACCCATTCA ACCTCAAAGT CCTTACCAAC CACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TGTACAGTGC CCGAGGAGTT GCAGAAAGAA CACCCAGCTT CATGTATCCC 
CAGGGCACTG CAAGCAGTCA GATGGTGCTG CGTGGCATGG ACCTCCTGCT GGAGTGCATC 
GCCTCCGGGG TCCCAACACC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCGTCT 
GACAAAGCCA AGTTTGAGAA CTTTAACAAG GCCCTGCGTA TCACCAACGT GTCCGAGGAA 
GACTCCGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT 
CCTGGCGAGG ACGGGAGACT GGTGTGTCGA GCCAACGGGA ACCCCAAGCC CACCGTCCAG 
TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCC 
GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GGGCCGTGTA CCAGTGCAAC 
ACCTCCAACG AGCACGGCTA CCTGCTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCG 
CCTCGGATGC TGTCACCCCG GAACCAGCTC ATCAGGGTGA TCCTGTACAA CCGGACGCGG 
CTGGACTGCC CGTTCTTTGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG CAACTACCAT GTCTACGAGA ACGGCAGTCT GGAAATCAAG 
ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTTG CCACCAACAT CCTGGGCAAA 
GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG CATGCCTGAG 
GACCAGGTCG CCAAAAGGGG CACCACGGTG CAGCTGGAGT GTCGCGTGAA GCACGACCCC 
TCCCTGAAGC TCACGGTGTA CTGGCTGAAG GACGACGAGC CCCTCTACGT CGGGAACAGG 
ATGAAGAAGG AAGAGGACTC CCTGACCATC TTTGGTGTGG CCGAGCGGGA CCAGGGTAGC 
TACACATGCG TTGCCAGCAC CGAGCTGGAC CAAGACCTGG CCAAGGCCTA CCTGACCGTG 
CTAGGACGGC CAGACCGACC CCGGGACCTG GAGCTCACCG ACCTGGCCGA GAGGAGCGTG 
AGACTGACCT GGATTCCGGG GGACGATAAC AACAGCCCCA TCACAGACTA CGTTGTCCAG 
TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGAAGT 
GTCAACTCAG CCATCCTCCA GCTGTCCCCC TATGTCAACT ACCAGTTCCG GGTCATTGCC 
ATCAATGAGG TGGGCAGCAG CCATCCCAGC CTCCCGTCCG AGCGCTACCG AACCAGTGGA 
GCACCCCCTG AGTCCAACCC CTCTGATGTG AAGGGAGAGG GGACCAGAAA GAACAACATG 
GAGATCACGT GGACGCCGAT GAACGCCACC TCCGCCTTTG GCCCCAACCT GCGCTACATC 
GTCAAGTGGC GGCGGAGAGA GACCCGAGAG ACCTGGAACA ATGTCACTGT GTGGGGCTCC 
CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCAGAA 
AATGACTTTG GGAAGGGCCC TGAACCAGAC ACTGTCATCG GTTACTCTGG AGAAGATTAT 
CCCAGAGCTG CACCCACTGA TGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC 
TACTGGAGGG AGAGCAGCTT GTTGAAGAAC CTGTGGGTGT CTCAGAAGAG GCAGCAAGCA 
GGCTTCCCCG GCGACCGCCT CCGTGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGCTGTGGT CAATGGGCGA GGCGATGGGC CTCGCAGTGA GACCAAGGAG 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCCGAGTCCG GCAGCCCAAC 
CTGGAGACAA TCAACCTGGA ATGGGATCAT CCAGAACACC CCAATGGGAT CCTGACTGGA 
TACACTCTCA AATACGTGGC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCACGATG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC 
TTTTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CAGTCACAGA GGAGTCACCA 
GCACCCCCGA ATGAAGCCTA CACCAACAAC CAGGCGGACA TCGCCACCCA GGGCTGGTTC 
ATTGGGCTCA TGTGTGCCGT CGCCCTCCTG GTGCTGACCC TGCTCATCGT CTGTTTCATC 
AAGAGGAGTC GAGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTTCC CCTTGGCCCT 
GAGGACCCCA AAGAAGAGGA TGGCTCCTTT GATTACAGTG ACGAGGACAA CAAGCCCTTG 
CAGGGCAGCC AGACGTCCCT GGATGGTACC ATCAAGCAGC AGGAGAGTGA TGACAGCCTG 
GTGGACTACG GCGAGGGCGG CGAGGGACAG TTCAATGAGG ACGGCTCCTT CATCGGCCAG 
TACACAGTCA AAAAGGACAA GGAAGAGACA GAGGGCAACG AAAGCTCAGA GGCCACATCT 
CCTGTCAACG CCATCTATTC TCTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NFASC cDNA ORF clone, Orcinus orca(killer whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

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FAX

オンラインフォーム