LOC110532149 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110532149
Entrez Gene ID	110532149
Full Name	TBC1 domain family member 25-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021615792.1	XP_021471467.1	TBC1 domain family member 25-like isoform X1
XM_021615793.1	XP_021471468.1	TBC1 domain family member 25-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC110532149 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110532149 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf07516	XM_021615792.1 Latest version!	Oncorhynchus mykiss TBC1 domain family member 25-like (LOC110532149), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOf07517	XM_021615793.1 Latest version!	Oncorhynchus mykiss TBC1 domain family member 25-like (LOC110532149), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110532149 ( XM_021615792.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021471467.1 Oncorhynchus mykiss TBC1 domain family member 25-like (LOC110532149), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf07516
Clone ID Related Accession (Same CDS sequence)	XM_021615792.1
Accession Version	XM_021615792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	TBC1 domain family member 25-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACTG AGGAGGAGAG GGGCGTAGTG CGTGTCAAAG TCAAGAAGTG GGAGGGGATG 
CTGCCAGTGG AGCTCCGCTC GTTTGCTGTG GATCCTCAGA TCACATCACT AGAGGTGCTA 
CAGCACATCC TCATCCGAGC CTTTGAGTTA AATGGCAAAC GCAACTTTGG GATAAGTTAC 
CTGTCCCAGG ACCGTGGAGG TGTGGAAGTG TATCTCTCTC TCCTTTCTGA CTGGGATTTA 
GAAGCTGCTT TTGTCAGTGC AGCTAAACCT TATCTACAGC TCAAGATGGA CATTAATCTC 
TCCGAAGACA GTCCCGTTAT GGAAGACTGG GACATAATAA GCCCCAAAGA TGTGATTGGT 
TCTGACCAGC TTCCTGGGGA TAGGAGGTCT TTGGCTGCTG CCGCCCTTCC CTTCACACAG 
TCCCTGCTCT CACAGATGAT GGGTCGAACG CTGTCTAGAG TACAGCAGGC CTTGAGCTGG 
TCGTATGGGG AGGAGGTGAA GCCTTTCAAG ACCCCTCTGA GCGATGCCGA GTTCCACAGT 
TACCTCAACG GCCAGGGCCA GCTGTCCCGG CCAGAGGAAC TGAGGCTACG TATCTATCAT 
GGTGGGGTGG AGCCCTCTCT ACGCAAGGTT GTCTGGCGAT ACCTCCTGAA CGTGTACCCC 
GATGGGCTGA CCGGTCAGGA GAGAATGGAC TACATGAAGG GCAAGACTAG GGAGTATGAC 
CAACTGAAGG GGGAGTGGTC AGCGAGGGCC AGTCCAGAGG ACCTGGAGTT CATCAGGGGC 
AACGTACTGA AGGACGTCCT GAGGACCGAC CGGGCCCATC CCTATTACGC CGGCTCCGAG 
GACAGCCCCC ACCTCACTGC TCTCACCGAC CTGCTCACCA CCTTTGCCAT TACACATCCA 
CAGGTGTCAT ACTGCCAAGG CATGAGTGAT ATTGCTTCTC CGATACTTGC CGTCATGGAC 
AATGAGGCAC ATGCATTCAT CTGTTTCTGT GGCATCATGA AACGCCTAGA GGGCAACTTC 
CGTCCGGATG GCCATTTCAT GTCCGTCAAG TTCCAGCATC TGAAGCTGCT ACTGCAGTAC 
TCAGACCCCG AGTTCTACTG TTATTTGGTG TCCCGCGGAG CTGATGACCT CTTCTTCTGT 
TACCGCTGGC TGCTCTTGGA ACTCAAACGG GAGTTTGCCT TTGACGATGC CCTGAGGATG 
CTGGAGGTCA CCTGGAGCTC CCTGCCCCCT GACCCTCCCG AAACAGAAGT GGAGCTTCTT 
GGGCCGCCGC TAGAAACAGA CCAAACCATA GTGACATGTG AGAGTGGTGT TGAAAAGAGG 
CCCGCCATTA CATGGGATGA TGGAAATGAA GGGGAGCTGA AAGAGAAGCA GCGGAGACGA 
CACATGTTGA GGCCCTCAAG AGAGGAGGCA GATGGGGGGA GAAAGATCAA AATGGAGGAT 
AGAGAGAGGG AGGACAGAGG AGCCAGAAGA GAGGGTGAGG GGAACTTTGA CATGCCTGAC 
GTGGGAAAGG AGAAGGCTCA CAACAACGTG CCTGTTCCTT CCTTCGAGAA GCAGACCAGC 
TTTGGAGAGT TCAAATACTA CAGTGCACGG AACAAAGACG GCTTTGATTT CGAGCAGACA 
GACCCACCAG TGGGTCCTGT TGAGATGGAA GCCGCCTCAC ACCCATGGGA GAGGGCGCCT 
CTTATAAAGG ACATTAACAA GTCTTCCAGC CCAGATACAG AGGTGAGGAG CTCCCCAAAT 
GGACAAATAG CCTCTCCGGT CTCCTCGTTT CCTCCTGGTC TGCCAAACTG GAAAAACGTT 
TCCTCTCTGT CTCCAGGCTC CAATCCCTCC CCCTCAAGTT GGAGAGGTGC CTCCCCGGAC 
ACCCCGTCAC CAAAGTTCAC CTCTCCCATT TTATCCAACG GAAGGATGGC GGTGCCGAAT 
GGAACCGGAG CCAACTCACC CGCTACCCCC ACAGGGAGGT CCTTAGTTTC CTCTCCGCTG 
TTGTCTCGAG CGTTGCTCTC CTCGCCAATT TTGTCCTTTG GAAGAGCTAT TCCTTCGCTG 
CCCGTTGGAC CGGTGAACAA GTCCTTTTCT AGTAACCTTC AATCTCCTTG CAGCAGAGCT 
TTCAGCACTG CGCCTCACAC TCCAAATTCA ACCAAACCAG AGAATACCAC CATCAAACCT 
TGTTCCCTGC CACCTCCTCA AGAGTTTGGC AAAGGAAATC CTTTCATGTT GTTCCTGTGC 
CTGTCGATCC TGCTGGAGCA TCGGGACCAC ATTATAAAGA ACGGCTTTGA TTACAACGAG 
CTGGCTATGC ACTTTGACCG CCTTGTTCGG CGACACAATC TGGGCAGGGT GCTGCAGCGT 
GCCAAGGCCT TGTTTGCAGA CTACCTGCAG AGTGAGGTAT GGGACTCAGA GGAAGGGGAT 
GAGGTCAGCT CGGATTCGCC CACCACAGTC ACTGCTGCTC TGTATTCCCC GCCCCCCTCT 
GCCACCAGGC TTCTTTTTAC CCCGTTGTCA TCCCCACAAC CATCCTCCCC AAACTCCACC 
TATAACCTGG CTGACACCAT ACCTTCCCCA TCACCTGTCT CCCTCTCTCC ATCCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021471467.1
CDS	123..2702
Translation	MTTEEERGVVRVKVKKWEGMLPVELRSFAVDPQITSLEVLQHILIRAFELNGKRNFGISYLSQDRGGVEVYLSLLSDWDLEAAFVSAAKPYLQLKMDINLSEDSPVMEDWDIISPKDVIGSDQLPGDRRSLAAAALPFTQSLLSQMMGRTLSRVQQALSWSYGEEVKPFKTPLSDAEFHSYLNGQGQLSRPEELRLRIYHGGVEPSLRKVVWRYLLNVYPDGLTGQERMDYMKGKTREYDQLKGEWSARASPEDLEFIRGNVLKDVLRTDRAHPYYAGSEDSPHLTALTDLLTTFAITHPQVSYCQGMSDIASPILAVMDNEAHAFICFCGIMKRLEGNFRPDGHFMSVKFQHLKLLLQYSDPEFYCYLVSRGADDLFFCYRWLLLELKREFAFDDALRMLEVTWSSLPPDPPETEVELLGPPLETDQTIVTCESGVEKRPAITWDDGNEGELKEKQRRRHMLRPSREEADGGRKIKMEDREREDRGARREGEGNFDMPDVGKEKAHNNVPVPSFEKQTSFGEFKYYSARNKDGFDFEQTDPPVGPVEMEAASHPWERAPLIKDINKSSSPDTEVRSSPNGQIASPVSSFPPGLPNWKNVSSLSPGSNPSPSSWRGASPDTPSPKFTSPILSNGRMAVPNGTGANSPATPTGRSLVSSPLLSRALLSSPILSFGRAIPSLPVGPVNKSFSSNLQSPCSRAFSTAPHTPNSTKPENTTIKPCSLPPPQEFGKGNPFMLFLCLSILLEHRDHIIKNGFDYNELAMHFDRLVRRHNLGRVLQRAKALFADYLQSEVWDSEEGDEVSSDSPTTVTAALYSPPPSATRLLFTPLSSPQPSSPNSTYNLADTIPSPSPVSLSPSS

Target ORF information:

RefSeq Version	XM_021615792.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss TBC1 domain family member 25-like (LOC110532149), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021615792.1

ORF Insert Sequence:

ATGACAACTG AGGAGGAGAG GGGCGTAGTG CGTGTCAAAG TCAAGAAGTG GGAGGGGATG 
CTGCCAGTGG AGCTCCGCTC GTTTGCTGTG GATCCTCAGA TCACATCACT AGAGGTGCTA 
CAGCACATCC TCATCCGAGC CTTTGAGTTA AATGGCAAAC GCAACTTTGG GATAAGTTAC 
CTGTCCCAGG ACCGTGGAGG TGTGGAAGTG TATCTCTCTC TCCTTTCTGA CTGGGATTTA 
GAAGCTGCTT TTGTCAGTGC AGCTAAACCT TATCTACAGC TCAAGATGGA CATTAATCTC 
TCCGAAGACA GTCCCGTTAT GGAAGACTGG GACATAATAA GCCCCAAAGA TGTGATTGGT 
TCTGACCAGC TTCCTGGGGA TAGGAGGTCT TTGGCTGCTG CCGCCCTTCC CTTCACACAG 
TCCCTGCTCT CACAGATGAT GGGTCGAACG CTGTCTAGAG TACAGCAGGC CTTGAGCTGG 
TCGTATGGGG AGGAGGTGAA GCCTTTCAAG ACCCCTCTGA GCGATGCCGA GTTCCACAGT 
TACCTCAACG GCCAGGGCCA GCTGTCCCGG CCAGAGGAAC TGAGGCTACG TATCTATCAT 
GGTGGGGTGG AGCCCTCTCT ACGCAAGGTT GTCTGGCGAT ACCTCCTGAA CGTGTACCCC 
GATGGGCTGA CCGGTCAGGA GAGAATGGAC TACATGAAGG GCAAGACTAG GGAGTATGAC 
CAACTGAAGG GGGAGTGGTC AGCGAGGGCC AGTCCAGAGG ACCTGGAGTT CATCAGGGGC 
AACGTACTGA AGGACGTCCT GAGGACCGAC CGGGCCCATC CCTATTACGC CGGCTCCGAG 
GACAGCCCCC ACCTCACTGC TCTCACCGAC CTGCTCACCA CCTTTGCCAT TACACATCCA 
CAGGTGTCAT ACTGCCAAGG CATGAGTGAT ATTGCTTCTC CGATACTTGC CGTCATGGAC 
AATGAGGCAC ATGCATTCAT CTGTTTCTGT GGCATCATGA AACGCCTAGA GGGCAACTTC 
CGTCCGGATG GCCATTTCAT GTCCGTCAAG TTCCAGCATC TGAAGCTGCT ACTGCAGTAC 
TCAGACCCCG AGTTCTACTG TTATTTGGTG TCCCGCGGAG CTGATGACCT CTTCTTCTGT 
TACCGCTGGC TGCTCTTGGA ACTCAAACGG GAGTTTGCCT TTGACGATGC CCTGAGGATG 
CTGGAGGTCA CCTGGAGCTC CCTGCCCCCT GACCCTCCCG AAACAGAAGT GGAGCTTCTT 
GGGCCGCCGC TAGAAACAGA CCAAACCATA GTGACATGTG AGAGTGGTGT TGAAAAGAGG 
CCCGCCATTA CATGGGATGA TGGAAATGAA GGGGAGCTGA AAGAGAAGCA GCGGAGACGA 
CACATGTTGA GGCCCTCAAG AGAGGAGGCA GATGGGGGGA GAAAGATCAA AATGGAGGAT 
AGAGAGAGGG AGGACAGAGG AGCCAGAAGA GAGGGTGAGG GGAACTTTGA CATGCCTGAC 
GTGGGAAAGG AGAAGGCTCA CAACAACGTG CCTGTTCCTT CCTTCGAGAA GCAGACCAGC 
TTTGGAGAGT TCAAATACTA CAGTGCACGG AACAAAGACG GCTTTGATTT CGAGCAGACA 
GACCCACCAG TGGGTCCTGT TGAGATGGAA GCCGCCTCAC ACCCATGGGA GAGGGCGCCT 
CTTATAAAGG ACATTAACAA GTCTTCCAGC CCAGATACAG AGGTGAGGAG CTCCCCAAAT 
GGACAAATAG CCTCTCCGGT CTCCTCGTTT CCTCCTGGTC TGCCAAACTG GAAAAACGTT 
TCCTCTCTGT CTCCAGGCTC CAATCCCTCC CCCTCAAGTT GGAGAGGTGC CTCCCCGGAC 
ACCCCGTCAC CAAAGTTCAC CTCTCCCATT TTATCCAACG GAAGGATGGC GGTGCCGAAT 
GGAACCGGAG CCAACTCACC CGCTACCCCC ACAGGGAGGT CCTTAGTTTC CTCTCCGCTG 
TTGTCTCGAG CGTTGCTCTC CTCGCCAATT TTGTCCTTTG GAAGAGCTAT TCCTTCGCTG 
CCCGTTGGAC CGGTGAACAA GTCCTTTTCT AGTAACCTTC AATCTCCTTG CAGCAGAGCT 
TTCAGCACTG CGCCTCACAC TCCAAATTCA ACCAAACCAG AGAATACCAC CATCAAACCT 
TGTTCCCTGC CACCTCCTCA AGAGTTTGGC AAAGGAAATC CTTTCATGTT GTTCCTGTGC 
CTGTCGATCC TGCTGGAGCA TCGGGACCAC ATTATAAAGA ACGGCTTTGA TTACAACGAG 
CTGGCTATGC ACTTTGACCG CCTTGTTCGG CGACACAATC TGGGCAGGGT GCTGCAGCGT 
GCCAAGGCCT TGTTTGCAGA CTACCTGCAG AGTGAGGTAT GGGACTCAGA GGAAGGGGAT 
GAGGTCAGCT CGGATTCGCC CACCACAGTC ACTGCTGCTC TGTATTCCCC GCCCCCCTCT 
GCCACCAGGC TTCTTTTTAC CCCGTTGTCA TCCCCACAAC CATCCTCCCC AAACTCCACC 
TATAACCTGG CTGACACCAT ACCTTCCCCA TCACCTGTCT CCCTCTCTCC ATCCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110532149 ( XM_021615793.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021471468.1 Oncorhynchus mykiss TBC1 domain family member 25-like (LOC110532149), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOf07517
Clone ID Related Accession (Same CDS sequence)	XM_021615793.1
Accession Version	XM_021615793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	TBC1 domain family member 25-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCAG TGGAGCTCCG CTCGTTTGCT GTGGATCCTC AGATCACATC ACTAGAGGTG 
CTACAGCACA TCCTCATCCG AGCCTTTGAG TTAAATGGCA AACGCAACTT TGGGATAAGT 
TACCTGTCCC AGGACCGTGG AGGTGTGGAA GTGTATCTCT CTCTCCTTTC TGACTGGGAT 
TTAGAAGCTG CTTTTGTCAG TGCAGCTAAA CCTTATCTAC AGCTCAAGAT GGACATTAAT 
CTCTCCGAAG ACAGTCCCGT TATGGAAGAC TGGGACATAA TAAGCCCCAA AGATGTGATT 
GGTTCTGACC AGCTTCCTGG GGATAGGAGG TCTTTGGCTG CTGCCGCCCT TCCCTTCACA 
CAGTCCCTGC TCTCACAGAT GATGGGTCGA ACGCTGTCTA GAGTACAGCA GGCCTTGAGC 
TGGTCGTATG GGGAGGAGGT GAAGCCTTTC AAGACCCCTC TGAGCGATGC CGAGTTCCAC 
AGTTACCTCA ACGGCCAGGG CCAGCTGTCC CGGCCAGAGG AACTGAGGCT ACGTATCTAT 
CATGGTGGGG TGGAGCCCTC TCTACGCAAG GTTGTCTGGC GATACCTCCT GAACGTGTAC 
CCCGATGGGC TGACCGGTCA GGAGAGAATG GACTACATGA AGGGCAAGAC TAGGGAGTAT 
GACCAACTGA AGGGGGAGTG GTCAGCGAGG GCCAGTCCAG AGGACCTGGA GTTCATCAGG 
GGCAACGTAC TGAAGGACGT CCTGAGGACC GACCGGGCCC ATCCCTATTA CGCCGGCTCC 
GAGGACAGCC CCCACCTCAC TGCTCTCACC GACCTGCTCA CCACCTTTGC CATTACACAT 
CCACAGGTGT CATACTGCCA AGGCATGAGT GATATTGCTT CTCCGATACT TGCCGTCATG 
GACAATGAGG CACATGCATT CATCTGTTTC TGTGGCATCA TGAAACGCCT AGAGGGCAAC 
TTCCGTCCGG ATGGCCATTT CATGTCCGTC AAGTTCCAGC ATCTGAAGCT GCTACTGCAG 
TACTCAGACC CCGAGTTCTA CTGTTATTTG GTGTCCCGCG GAGCTGATGA CCTCTTCTTC 
TGTTACCGCT GGCTGCTCTT GGAACTCAAA CGGGAGTTTG CCTTTGACGA TGCCCTGAGG 
ATGCTGGAGG TCACCTGGAG CTCCCTGCCC CCTGACCCTC CCGAAACAGA AGTGGAGCTT 
CTTGGGCCGC CGCTAGAAAC AGACCAAACC ATAGTGACAT GTGAGAGTGG TGTTGAAAAG 
AGGCCCGCCA TTACATGGGA TGATGGAAAT GAAGGGGAGC TGAAAGAGAA GCAGCGGAGA 
CGACACATGT TGAGGCCCTC AAGAGAGGAG GCAGATGGGG GGAGAAAGAT CAAAATGGAG 
GATAGAGAGA GGGAGGACAG AGGAGCCAGA AGAGAGGGTG AGGGGAACTT TGACATGCCT 
GACGTGGGAA AGGAGAAGGC TCACAACAAC GTGCCTGTTC CTTCCTTCGA GAAGCAGACC 
AGCTTTGGAG AGTTCAAATA CTACAGTGCA CGGAACAAAG ACGGCTTTGA TTTCGAGCAG 
ACAGACCCAC CAGTGGGTCC TGTTGAGATG GAAGCCGCCT CACACCCATG GGAGAGGGCG 
CCTCTTATAA AGGACATTAA CAAGTCTTCC AGCCCAGATA CAGAGGTGAG GAGCTCCCCA 
AATGGACAAA TAGCCTCTCC GGTCTCCTCG TTTCCTCCTG GTCTGCCAAA CTGGAAAAAC 
GTTTCCTCTC TGTCTCCAGG CTCCAATCCC TCCCCCTCAA GTTGGAGAGG TGCCTCCCCG 
GACACCCCGT CACCAAAGTT CACCTCTCCC ATTTTATCCA ACGGAAGGAT GGCGGTGCCG 
AATGGAACCG GAGCCAACTC ACCCGCTACC CCCACAGGGA GGTCCTTAGT TTCCTCTCCG 
CTGTTGTCTC GAGCGTTGCT CTCCTCGCCA ATTTTGTCCT TTGGAAGAGC TATTCCTTCG 
CTGCCCGTTG GACCGGTGAA CAAGTCCTTT TCTAGTAACC TTCAATCTCC TTGCAGCAGA 
GCTTTCAGCA CTGCGCCTCA CACTCCAAAT TCAACCAAAC CAGAGAATAC CACCATCAAA 
CCTTGTTCCC TGCCACCTCC TCAAGAGTTT GGCAAAGGAA ATCCTTTCAT GTTGTTCCTG 
TGCCTGTCGA TCCTGCTGGA GCATCGGGAC CACATTATAA AGAACGGCTT TGATTACAAC 
GAGCTGGCTA TGCACTTTGA CCGCCTTGTT CGGCGACACA ATCTGGGCAG GGTGCTGCAG 
CGTGCCAAGG CCTTGTTTGC AGACTACCTG CAGAGTGAGG TATGGGACTC AGAGGAAGGG 
GATGAGGTCA GCTCGGATTC GCCCACCACA GTCACTGCTG CTCTGTATTC CCCGCCCCCC 
TCTGCCACCA GGCTTCTTTT TACCCCGTTG TCATCCCCAC AACCATCCTC CCCAAACTCC 
ACCTATAACC TGGCTGACAC CATACCTTCC CCATCACCTG TCTCCCTCTC TCCATCCTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021471468.1
CDS	211..2733
Translation	MLPVELRSFAVDPQITSLEVLQHILIRAFELNGKRNFGISYLSQDRGGVEVYLSLLSDWDLEAAFVSAAKPYLQLKMDINLSEDSPVMEDWDIISPKDVIGSDQLPGDRRSLAAAALPFTQSLLSQMMGRTLSRVQQALSWSYGEEVKPFKTPLSDAEFHSYLNGQGQLSRPEELRLRIYHGGVEPSLRKVVWRYLLNVYPDGLTGQERMDYMKGKTREYDQLKGEWSARASPEDLEFIRGNVLKDVLRTDRAHPYYAGSEDSPHLTALTDLLTTFAITHPQVSYCQGMSDIASPILAVMDNEAHAFICFCGIMKRLEGNFRPDGHFMSVKFQHLKLLLQYSDPEFYCYLVSRGADDLFFCYRWLLLELKREFAFDDALRMLEVTWSSLPPDPPETEVELLGPPLETDQTIVTCESGVEKRPAITWDDGNEGELKEKQRRRHMLRPSREEADGGRKIKMEDREREDRGARREGEGNFDMPDVGKEKAHNNVPVPSFEKQTSFGEFKYYSARNKDGFDFEQTDPPVGPVEMEAASHPWERAPLIKDINKSSSPDTEVRSSPNGQIASPVSSFPPGLPNWKNVSSLSPGSNPSPSSWRGASPDTPSPKFTSPILSNGRMAVPNGTGANSPATPTGRSLVSSPLLSRALLSSPILSFGRAIPSLPVGPVNKSFSSNLQSPCSRAFSTAPHTPNSTKPENTTIKPCSLPPPQEFGKGNPFMLFLCLSILLEHRDHIIKNGFDYNELAMHFDRLVRRHNLGRVLQRAKALFADYLQSEVWDSEEGDEVSSDSPTTVTAALYSPPPSATRLLFTPLSSPQPSSPNSTYNLADTIPSPSPVSLSPSS

Target ORF information:

RefSeq Version	XM_021615793.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss TBC1 domain family member 25-like (LOC110532149), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021615793.1

ORF Insert Sequence:

ATGCTGCCAG TGGAGCTCCG CTCGTTTGCT GTGGATCCTC AGATCACATC ACTAGAGGTG 
CTACAGCACA TCCTCATCCG AGCCTTTGAG TTAAATGGCA AACGCAACTT TGGGATAAGT 
TACCTGTCCC AGGACCGTGG AGGTGTGGAA GTGTATCTCT CTCTCCTTTC TGACTGGGAT 
TTAGAAGCTG CTTTTGTCAG TGCAGCTAAA CCTTATCTAC AGCTCAAGAT GGACATTAAT 
CTCTCCGAAG ACAGTCCCGT TATGGAAGAC TGGGACATAA TAAGCCCCAA AGATGTGATT 
GGTTCTGACC AGCTTCCTGG GGATAGGAGG TCTTTGGCTG CTGCCGCCCT TCCCTTCACA 
CAGTCCCTGC TCTCACAGAT GATGGGTCGA ACGCTGTCTA GAGTACAGCA GGCCTTGAGC 
TGGTCGTATG GGGAGGAGGT GAAGCCTTTC AAGACCCCTC TGAGCGATGC CGAGTTCCAC 
AGTTACCTCA ACGGCCAGGG CCAGCTGTCC CGGCCAGAGG AACTGAGGCT ACGTATCTAT 
CATGGTGGGG TGGAGCCCTC TCTACGCAAG GTTGTCTGGC GATACCTCCT GAACGTGTAC 
CCCGATGGGC TGACCGGTCA GGAGAGAATG GACTACATGA AGGGCAAGAC TAGGGAGTAT 
GACCAACTGA AGGGGGAGTG GTCAGCGAGG GCCAGTCCAG AGGACCTGGA GTTCATCAGG 
GGCAACGTAC TGAAGGACGT CCTGAGGACC GACCGGGCCC ATCCCTATTA CGCCGGCTCC 
GAGGACAGCC CCCACCTCAC TGCTCTCACC GACCTGCTCA CCACCTTTGC CATTACACAT 
CCACAGGTGT CATACTGCCA AGGCATGAGT GATATTGCTT CTCCGATACT TGCCGTCATG 
GACAATGAGG CACATGCATT CATCTGTTTC TGTGGCATCA TGAAACGCCT AGAGGGCAAC 
TTCCGTCCGG ATGGCCATTT CATGTCCGTC AAGTTCCAGC ATCTGAAGCT GCTACTGCAG 
TACTCAGACC CCGAGTTCTA CTGTTATTTG GTGTCCCGCG GAGCTGATGA CCTCTTCTTC 
TGTTACCGCT GGCTGCTCTT GGAACTCAAA CGGGAGTTTG CCTTTGACGA TGCCCTGAGG 
ATGCTGGAGG TCACCTGGAG CTCCCTGCCC CCTGACCCTC CCGAAACAGA AGTGGAGCTT 
CTTGGGCCGC CGCTAGAAAC AGACCAAACC ATAGTGACAT GTGAGAGTGG TGTTGAAAAG 
AGGCCCGCCA TTACATGGGA TGATGGAAAT GAAGGGGAGC TGAAAGAGAA GCAGCGGAGA 
CGACACATGT TGAGGCCCTC AAGAGAGGAG GCAGATGGGG GGAGAAAGAT CAAAATGGAG 
GATAGAGAGA GGGAGGACAG AGGAGCCAGA AGAGAGGGTG AGGGGAACTT TGACATGCCT 
GACGTGGGAA AGGAGAAGGC TCACAACAAC GTGCCTGTTC CTTCCTTCGA GAAGCAGACC 
AGCTTTGGAG AGTTCAAATA CTACAGTGCA CGGAACAAAG ACGGCTTTGA TTTCGAGCAG 
ACAGACCCAC CAGTGGGTCC TGTTGAGATG GAAGCCGCCT CACACCCATG GGAGAGGGCG 
CCTCTTATAA AGGACATTAA CAAGTCTTCC AGCCCAGATA CAGAGGTGAG GAGCTCCCCA 
AATGGACAAA TAGCCTCTCC GGTCTCCTCG TTTCCTCCTG GTCTGCCAAA CTGGAAAAAC 
GTTTCCTCTC TGTCTCCAGG CTCCAATCCC TCCCCCTCAA GTTGGAGAGG TGCCTCCCCG 
GACACCCCGT CACCAAAGTT CACCTCTCCC ATTTTATCCA ACGGAAGGAT GGCGGTGCCG 
AATGGAACCG GAGCCAACTC ACCCGCTACC CCCACAGGGA GGTCCTTAGT TTCCTCTCCG 
CTGTTGTCTC GAGCGTTGCT CTCCTCGCCA ATTTTGTCCT TTGGAAGAGC TATTCCTTCG 
CTGCCCGTTG GACCGGTGAA CAAGTCCTTT TCTAGTAACC TTCAATCTCC TTGCAGCAGA 
GCTTTCAGCA CTGCGCCTCA CACTCCAAAT TCAACCAAAC CAGAGAATAC CACCATCAAA 
CCTTGTTCCC TGCCACCTCC TCAAGAGTTT GGCAAAGGAA ATCCTTTCAT GTTGTTCCTG 
TGCCTGTCGA TCCTGCTGGA GCATCGGGAC CACATTATAA AGAACGGCTT TGATTACAAC 
GAGCTGGCTA TGCACTTTGA CCGCCTTGTT CGGCGACACA ATCTGGGCAG GGTGCTGCAG 
CGTGCCAAGG CCTTGTTTGC AGACTACCTG CAGAGTGAGG TATGGGACTC AGAGGAAGGG 
GATGAGGTCA GCTCGGATTC GCCCACCACA GTCACTGCTG CTCTGTATTC CCCGCCCCCC 
TCTGCCACCA GGCTTCTTTT TACCCCGTTG TCATCCCCAC AACCATCCTC CCCAAACTCC 
ACCTATAACC TGGCTGACAC CATACCTTCC CCATCACCTG TCTCCCTCTC TCCATCCTCT 
TGA