il21 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	il21
Entrez Gene ID	100653437
Full Name	interleukin 21
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001246331.1	NP_001233260.1	interleukin 21 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Functional characterization of a nonmammalian IL-21: rainbow trout Oncorhynchus mykiss IL-21 upregulates the expression of the Th cell signature cytokines IFN-gamma, IL-10, and IL-22.
Wang T, Diaz-Rosales P, Costa MM, Campbell S, Snow M, Collet B, Martin SA, Secombes CJ
Journal of immunology (Baltimore, Md. : 1950)186(2)708-21(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following il21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf00666	NM_001246331.1 Latest version!	Oncorhynchus mykiss interleukin 21 (il21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il21 ( NM_001246331.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001233260.1 Oncorhynchus mykiss interleukin 21 (il21), mRNA.

Price & Availability↑

CloneID	OOf00666
Clone ID Related Accession (Same CDS sequence)	NM_001246331.1
Accession Version	NM_001246331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Oncorhynchus mykiss(rainbow trout)
Product	interleukin 21 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FM883701.1. ##Evidence-Data-START## Transcript exon combination :: FM883701.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00041049, SAMN07203783 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCTGC TTGTTTGTTA TCTCCTAGCC ATCACCTGTG TATTGGTGGA TGCAGACAAG 
GGTGAGAGAA TCCTGAAGCT GACTGAAGTA ATAAAGGAAT TAAAAGCGCT AAACAGAACT 
GTGACGCACA ACAGTGTGAT GTCGAACGCT CCTTCAATGG ATACGGAAGA GTGCTGTTCC 
CAGTCTGCCT TGAAGTGCTT CCGGGCGATG GTACCTCATC TCAAGGCCAA AAATAAGACC 
CTTCAGCGTA AAGTTATCAA AAACCTCAAC AACCGAATGA TTTTGGGAAG TCTGGACTCC 
TGCAGTCAGG AGGAGAGAGA GAAAACAGTC TGCCAAGGAT GTGATTCATA TCCAAAGGAC 
AGCCAGAAAT GTGTACAACA GTTGGAATCT CTGCTTCAAA AGGCCATCAG TAGACTGGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001233260.1
CDS	71..493
Translation	MKLLVCYLLAITCVLVDADKGERILKLTEVIKELKALNRTVTHNSVMSNAPSMDTEECCSQSALKCFRAMVPHLKAKNKTLQRKVIKNLNNRMILGSLDSCSQEEREKTVCQGCDSYPKDSQKCVQQLESLLQKAISRLA

Target ORF information:

RefSeq Version	NM_001246331.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss interleukin 21 (il21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001246331.1

ORF Insert Sequence:

ATGAAGCTGC TTGTTTGTTA TCTCCTAGCC ATCACCTGTG TATTGGTGGA TGCAGACAAG 
GGTGAGAGAA TCCTGAAGCT GACTGAAGTA ATAAAGGAAT TAAAAGCGCT AAACAGAACT 
GTGACGCACA ACAGTGTGAT GTCGAACGCT CCTTCAATGG ATACGGAAGA GTGCTGTTCC 
CAGTCTGCCT TGAAGTGCTT CCGGGCGATG GTACCTCATC TCAAGGCCAA AAATAAGACC 
CTTCAGCGTA AAGTTATCAA AAACCTCAAC AACCGAATGA TTTTGGGAAG TCTGGACTCC 
TGCAGTCAGG AGGAGAGAGA GAAAACAGTC TGCCAAGGAT GTGATTCATA TCCAAAGGAC 
AGCCAGAAAT GTGTACAACA GTTGGAATCT CTGCTTCAAA AGGCCATCAG TAGACTGGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional characterization of a nonmammalian IL-21: rainbow trout Oncorhynchus mykiss IL-21 upregulates the expression of the Th cell signature cytokines IFN-gamma, IL-10, and IL-22.
Journal of immunology (Baltimore, Md. : 1950)186(2)708-21(2011 Jan)
Wang T,Diaz-Rosales P,Costa MM,Campbell S,Snow M,Collet B,Martin SA,Secombes CJ