il12b cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	il12b
Entrez Gene ID	100136086
Full Name	interleukin-12 beta chain
Synonyms	il-12B1
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001124392.1	NP_001117864.1	interleukin-12 beta chain precursor

Pathways from BioSystems
Gene Ontology

The following il12b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il12b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf01137	NM_001124392.1 Latest version!	Oncorhynchus mykiss interleukin-12 beta chain (il12b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il12b ( NM_001124392.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001117864.1 Oncorhynchus mykiss interleukin-12 beta chain (il12b), mRNA.

Price & Availability↑

CloneID	OOf01137
Clone ID Related Accession (Same CDS sequence)	NM_001124392.1
Accession Version	NM_001124392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-05
Organism	Oncorhynchus mykiss(rainbow trout)
Product	interleukin-12 beta chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ548829.1. ##Evidence-Data-START## Transcript exon combination :: AJ548829.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00041048, SAMD00041049 [ECO:0000348] ##Evidence-Data-END##

ATGACTGTAG TACCATGGCT CATCATGCTC CTCTGTGTTA TTCTACCGAC AGTGAATGGT 
CTCACCTCCT TCCATGAACT CTACATGGTT GATAAGGGGG ACAGTTTGGT GACTCTGACA 
TGTCCCACGG CATTCTCTGG AGCTATGACG TGGAAGTACG AGGGAGAGAC TGAGGATTTA 
GACAAAACTC TGGTCCAAGG CCGTAACCTG ATACTGAATG AGGTGGACTG GTATGGAGAA 
TACAGCTGCT GGGATGGAGA CAAGAAACTA GGATCCATCT ATCTACTGGA GGAGCTGAAG 
AACCCAGACG ACGATGATTT AATCAGCTGT CGGGCCAAGT CTTATGGCTG CACCTTTAAC 
TGTAGCTGGA CCCACAGTGA ATTCACAGCT GTCCGTCTTG GGCTGGGCAA TGACTGTACT 
GACAGCCAGG AATCTTGCAC CTGGGTGTAC CCAACGACCA GCCTCCAAGA TGGAGGGTTG 
GAGTTTGAAC TGACACATTC GCTTTCACCC TACACTGAGG AGGCCGCTCC TCTAGTGGTC 
ACTGTTGAGG CCATCACCAG GCAGGATTAC CTCAGGAAGA CTAAGAGGTT CTACCTACAC 
GACATTGTCC AGCCAGACAG TCCTGCAGGG ATGAAGTGCC AGGTGATGGA TCAGAGATTG 
AAGGTGAAAG TAGAACCGCC AGCCACCTGG AACTGTCCAC CCAGCTACTT CCCTCTGGAG 
CACCAGATAG GATACATTTT TAAAGACAAC GGAAAGGAGG AGCACTCTAC GAATCCTCTC 
ATACCGAGAG GGGTCAGTAA GCTGAGGGCC CGCTCCAGAG ACCCCCTGGT CCCTTCCCCC 
TGGAGCCAGT GGACCCCCTG GAAAAATGTA ACCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001117864.1
CDS	31..909
Translation	MTVVPWLIMLLCVILPTVNGLTSFHELYMVDKGDSLVTLTCPTAFSGAMTWKYEGETEDLDKTLVQGRNLILNEVDWYGEYSCWDGDKKLGSIYLLEELKNPDDDDLISCRAKSYGCTFNCSWTHSEFTAVRLGLGNDCTDSQESCTWVYPTTSLQDGGLEFELTHSLSPYTEEAAPLVVTVEAITRQDYLRKTKRFYLHDIVQPDSPAGMKCQVMDQRLKVKVEPPATWNCPPSYFPLEHQIGYIFKDNGKEEHSTNPLIPRGVSKLRARSRDPLVPSPWSQWTPWKNVTN

Target ORF information:

RefSeq Version	NM_001124392.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss interleukin-12 beta chain (il12b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124392.1

ORF Insert Sequence:

ATGACTGTAG TACCATGGCT CATCATGCTC CTCTGTGTTA TTCTACCGAC AGTGAATGGT 
CTCACCTCCT TCCATGAACT CTACATGGTT GATAAGGGGG ACAGTTTGGT GACTCTGACA 
TGTCCCACGG CATTCTCTGG AGCTATGACG TGGAAGTACG AGGGAGAGAC TGAGGATTTA 
GACAAAACTC TGGTCCAAGG CCGTAACCTG ATACTGAATG AGGTGGACTG GTATGGAGAA 
TACAGCTGCT GGGATGGAGA CAAGAAACTA GGATCCATCT ATCTACTGGA GGAGCTGAAG 
AACCCAGACG ACGATGATTT AATCAGCTGT CGGGCCAAGT CTTATGGCTG CACCTTTAAC 
TGTAGCTGGA CCCACAGTGA ATTCACAGCT GTCCGTCTTG GGCTGGGCAA TGACTGTACT 
GACAGCCAGG AATCTTGCAC CTGGGTGTAC CCAACGACCA GCCTCCAAGA TGGAGGGTTG 
GAGTTTGAAC TGACACATTC GCTTTCACCC TACACTGAGG AGGCCGCTCC TCTAGTGGTC 
ACTGTTGAGG CCATCACCAG GCAGGATTAC CTCAGGAAGA CTAAGAGGTT CTACCTACAC 
GACATTGTCC AGCCAGACAG TCCTGCAGGG ATGAAGTGCC AGGTGATGGA TCAGAGATTG 
AAGGTGAAAG TAGAACCGCC AGCCACCTGG AACTGTCCAC CCAGCTACTT CCCTCTGGAG 
CACCAGATAG GATACATTTT TAAAGACAAC GGAAAGGAGG AGCACTCTAC GAATCCTCTC 
ATACCGAGAG GGGTCAGTAA GCTGAGGGCC CGCTCCAGAG ACCCCCTGGT CCCTTCCCCC 
TGGAGCCAGT GGACCCCCTG GAAAAATGTA ACCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.