il-8 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	il-8
Entrez Gene ID	100136039
Full Name	cxc chemokine-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001124362.1	NP_001117834.1	putative cxc chemokine precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The proximal promoter of a novel interleukin-8-encoding gene in rainbow trout (Oncorhynchus mykiss) is strongly induced by CEBPA, but not NF-?B p65.
Rebl A, Rebl H, Koryt?? T, Goldammer T, Seyfert HM
Developmental and comparative immunology46(2)155-64(2014 Oct)

3D modelling of three pro-inflammatory molecules in selected fish species.
Costantini S, Facchiano AM, Randelli E, Casani D, Scapigliati G, Buonocore F
Current pharmaceutical design16(38)4203-12(2010)

Genomic cloning of novel isotypes of the rainbow trout interleukin-8.
Fujiki K, Gauley J, Bols NC, Dixon B
Immunogenetics55(2)126-31(2003 May)

Molecular cloning of an IL-8-like CXC chemokine and tissue factor in rainbow trout (Oncorhynchus mykiss) by use of suppression subtractive hybridization.
Sangrador-Vegas A, Lennington JB, Smith TJ
Cytokine17(2)66-70(2002 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Oncorhynchus mykiss CEBPA regulates expression of a novel IL-8 encoding gene.
Title: The proximal promoter of a novel interleukin-8-encoding gene in rainbow trout (Oncorhynchus mykiss) is strongly induced by CEBPA, but not NF-?B p65.

The predicted 3D structures of IL-1beta, TNF-alpha, and IL-8 from Oncorhynchus mykiss, Dicentrarchus labrax, and Chionodraco hamatus are reported and the differences between them and their mammalian counterparts investigated.
Title: 3D modelling of three pro-inflammatory molecules in selected fish species.

The following il-8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il-8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf00051	NM_001124362.1 Latest version!	Oncorhynchus mykiss cxc chemokine-like (il-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il-8 ( NM_001124362.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001117834.1 Oncorhynchus mykiss cxc chemokine-like (il-8), mRNA.

Price & Availability↑

CloneID	OOf00051
Clone ID Related Accession (Same CDS sequence)	NM_001124362.1
Accession Version	NM_001124362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Oncorhynchus mykiss(rainbow trout)
Product	putative cxc chemokine precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ300835.1. ##Evidence-Data-START## Transcript exon combination :: AJ300835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN06640878 [ECO:0000348] ##Evidence-Data-END##

ATGAGCATCA GAATGTCAGC CAGCCTTGTC GTTGTGCTCC TGGCCCTCCT GACCATTACT 
GAGGGGATGA GTCTGAGAGG CATGGGGGCT GACCTGCGAT GTCGCTGCAT TGAGACGGAA 
AGCAGACGAA TTGGTAAACT CATTAAGAAG GTGGAGATGT TCCCTCCCAG CTCGCACTGC 
AGAGACACTG AGATCATTGC CACTCTGAGC AAGAGCGGTC AGGAGATTTG TCTGGATGTC 
AGCGCTCCGT GGGTCAAGAG GGTCATTGAG AAGATGCTGG CCAACAACAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001117834.1
CDS	175..468
Translation	MSIRMSASLVVVLLALLTITEGMSLRGMGADLRCRCIETESRRIGKLIKKVEMFPPSSHCRDTEIIATLSKSGQEICLDVSAPWVKRVIEKMLANNK

Target ORF information:

RefSeq Version	NM_001124362.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss cxc chemokine-like (il-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124362.1

ORF Insert Sequence:

ATGAGCATCA GAATGTCAGC CAGCCTTGTC GTTGTGCTCC TGGCCCTCCT GACCATTACT 
GAGGGGATGA GTCTGAGAGG CATGGGGGCT GACCTGCGAT GTCGCTGCAT TGAGACGGAA 
AGCAGACGAA TTGGTAAACT CATTAAGAAG GTGGAGATGT TCCCTCCCAG CTCGCACTGC 
AGAGACACTG AGATCATTGC CACTCTGAGC AAGAGCGGTC AGGAGATTTG TCTGGATGTC 
AGCGCTCCGT GGGTCAAGAG GGTCATTGAG AAGATGCTGG CCAACAACAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The proximal promoter of a novel interleukin-8-encoding gene in rainbow trout (Oncorhynchus mykiss) is strongly induced by CEBPA, but not NF-?B p65.
Developmental and comparative immunology46(2)155-64(2014 Oct)
Rebl A,Rebl H,Koryt?? T,Goldammer T,Seyfert HM

3D modelling of three pro-inflammatory molecules in selected fish species.
Current pharmaceutical design16(38)4203-12(2010)
Costantini S,Facchiano AM,Randelli E,Casani D,Scapigliati G,Buonocore F

Genomic cloning of novel isotypes of the rainbow trout interleukin-8.
Immunogenetics55(2)126-31(2003 May)
Fujiki K,Gauley J,Bols NC,Dixon B

Molecular cloning of an IL-8-like CXC chemokine and tissue factor in rainbow trout (Oncorhynchus mykiss) by use of suppression subtractive hybridization.
Cytokine17(2)66-70(2002 Jan)
Sangrador-Vegas A,Lennington JB,Smith TJ