LOC100135979 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100135979
Entrez Gene ID	100135979
Full Name	CC chemokine with stalk CK2
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001124317.1	NP_001117789.1	CC chemokine with stalk CK2 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches.
Salem M, Rexroad CE 3rd, Wang J, Thorgaard GH, Yao J
BMC genomics11564(2010 Oct)

Cloning of a novel rainbow trout (Oncorhynchus mykiss) CC chemokine with a fractalkine-like stalk and a TNF decoy receptor using cDNA fragments containing AU-rich elements.
Liu L, Fujiki K, Dixon B, Sundick RS
Cytokine17(2)71-81(2002 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC100135979 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100135979 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf01126	NM_001124317.1 Latest version!	Oncorhynchus mykiss CC chemokine with stalk CK2 (LOC100135979), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100135979 ( NM_001124317.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001117789.1 Oncorhynchus mykiss CC chemokine with stalk CK2 (LOC100135979), mRNA.

Price & Availability↑

CloneID	OOf01126
Clone ID Related Accession (Same CDS sequence)	NM_001124317.1
Accession Version	NM_001124317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-09
Organism	Oncorhynchus mykiss(rainbow trout)
Product	CC chemokine with stalk CK2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF418561.1. ##Evidence-Data-START## Transcript exon combination :: AF418561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN06640878, SAMN06640882 [ECO:0000348] ##Evidence-Data-END##

ATGGTGACCT GTGGAACTCT GGTGAAGATC TGGACATTAG CCGTCGTCAT AGCAGCACTG 
GGCTGGACTG GGACAGTAGA TGCAGAAAAG CTGGTGTCGT GCTGTAAGAC AGTGTCTAGG 
ACAGAGGTGA ACGATCCCAT CACAGGCTAC TGGATTCAGA ACTATAACGC TCCTTGTGTA 
CGGGCCGTCA TCTTTGAGAC GAAGAAGGGT CTGTTCTGTA GTTACCATAA ACAACCCTGG 
GTACGCCGCA AGATTCATCA GTTTGAAATG GCCCGTCTGA GCTCAACATT TCTCTCGCTC 
TCCATTCCAA ACTCTCTCAC CTCCACCTCC ACCCCTACCA CTACCTCCTT ACCCTCCTCT 
CCACCCTCCT CTCTTTTCCC TCTTTCCTCC TCTTCTCCAT CCGTACCTTC CTCTCCTCCC 
TCTCTTTCCT CCTCTCCTTC CCATCTCCTC TCCTCTCTTT TCCCTGCCTC CTCCTCTCCT 
CCCTCTATCT CTTCCTCTCC TCCCTCTTTC TCCTCCCCCC GTCATTGGGA ATCGACCAAG 
AATGCCTCAA CTCAGCAGTC AACATCCAAC CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001117789.1
CDS	64..639
Translation	MVTCGTLVKIWTLAVVIAALGWTGTVDAEKLVSCCKTVSRTEVNDPITGYWIQNYNAPCVRAVIFETKKGLFCSYHKQPWVRRKIHQFEMARLSSTFLSLSIPNSLTSTSTPTTTSLPSSPPSSLFPLSSSSPSVPSSPPSLSSSPSHLLSSLFPASSSPPSISSSPPSFSSPRHWESTKNASTQQSTSNQ

Target ORF information:

RefSeq Version	NM_001124317.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss CC chemokine with stalk CK2 (LOC100135979), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124317.1

ORF Insert Sequence:

ATGGTGACCT GTGGAACTCT GGTGAAGATC TGGACATTAG CCGTCGTCAT AGCAGCACTG 
GGCTGGACTG GGACAGTAGA TGCAGAAAAG CTGGTGTCGT GCTGTAAGAC AGTGTCTAGG 
ACAGAGGTGA ACGATCCCAT CACAGGCTAC TGGATTCAGA ACTATAACGC TCCTTGTGTA 
CGGGCCGTCA TCTTTGAGAC GAAGAAGGGT CTGTTCTGTA GTTACCATAA ACAACCCTGG 
GTACGCCGCA AGATTCATCA GTTTGAAATG GCCCGTCTGA GCTCAACATT TCTCTCGCTC 
TCCATTCCAA ACTCTCTCAC CTCCACCTCC ACCCCTACCA CTACCTCCTT ACCCTCCTCT 
CCACCCTCCT CTCTTTTCCC TCTTTCCTCC TCTTCTCCAT CCGTACCTTC CTCTCCTCCC 
TCTCTTTCCT CCTCTCCTTC CCATCTCCTC TCCTCTCTTT TCCCTGCCTC CTCCTCTCCT 
CCCTCTATCT CTTCCTCTCC TCCCTCTTTC TCCTCCCCCC GTCATTGGGA ATCGACCAAG 
AATGCCTCAA CTCAGCAGTC AACATCCAAC CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches.
BMC genomics11564(2010 Oct)
Salem M,Rexroad CE 3rd,Wang J,Thorgaard GH,Yao J

Cloning of a novel rainbow trout (Oncorhynchus mykiss) CC chemokine with a fractalkine-like stalk and a TNF decoy receptor using cDNA fragments containing AU-rich elements.
Cytokine17(2)71-81(2002 Jan)
Liu L,Fujiki K,Dixon B,Sundick RS