bard1 cDNA ORF clone, Oncorhynchus kisutch(coho salmon)

Gene
Clones
gRNAs

Summary

Gene Symbol	bard1
Entrez Gene ID	109871367
Full Name	BRCA1 associated RING domain 1
Gene Type	protein-coding
Organism	Oncorhynchus kisutch(coho salmon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020462219.2	XP_020317808.1	BRCA1-associated RING domain protein 1
XM_020462219.1	XP_020317808.1	BRCA1-associated RING domain protein 1

Pathways from BioSystems
Gene Ontology

The following bard1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bard1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOn44285	XM_020462219.2 Latest version!	Oncorhynchus kisutch BRCA1 associated RING domain 1 (bard1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OOn44285	XM_020462219.1	Oncorhynchus kisutch BRCA1 associated RING domain 1 (bard1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bard1 ( XM_020462219.2 ) cDNA ORF clone, Oncorhynchus kisutch(coho salmon) -> XP_020317808.1 Oncorhynchus kisutch BRCA1 associated RING domain 1 (bard1), mRNA.

Price & Availability↑

CloneID	OOn44285
Clone ID Related Accession (Same CDS sequence)	XM_020462219.2 , XM_020462219.1
Accession Version	XM_020462219.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-08
Organism	Oncorhynchus kisutch(coho salmon)
Product	BRCA1-associated RING domain protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034199.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2019 this sequence version replaced XM_020462219.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oncorhynchus kisutch Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTG ATCTTTGCAC GGACGAAGGC TGGACGAAAA CGAAGGAGGC TGTCGCGCAT 
TTTCGAAAAC TTTTGTTGTG CTCAAAATGT TCAAAACTGA TGAACGAACC TGTGTGCCTC 
GGGGTTTGTG AACATATGCT TTGCAGGTCG TGTGCTGGTC CTCAGGCTGG AGAGGGATGT 
GTGGTGTGTC ACAGTCCTGC CTGGGTGAAA GACATTCGGA TCAACAGACA ACTCAGCAAT 
ATCACAGAAC TCTATGGCAA CCTAGACTCA CTGCTCAATC CCACTGGCTC CGATTATTCT 
GATGCCCTTC CCATTACACC ACCAGCACAG TCAGATAAAT CTGTCTTAAA GCACAAGAAG 
AACTTCAAGA TCTGGTTTAG TCCCCGCAGC CGCAAGGTGC GCTGCCGGGT AGAGAAGCCT 
GCGGCGGTGC CTCTCCCGGA GGTTGGGAGT GGGACTGTGA AAGCTGCTCC AAGACATCCT 
GAGACACTAC CTGAGTCTAA AGGCAAAGAC CTGTCTGTCT TCAACTTCAA CTCCTCCTCC 
CAAGACTCTG GCTCCTCCTC TCCTCAAAGG GCGACTAACT ATGAAAAGAA GAAGGCGACT 
AAGAAGACGA GAAATGGTGG TGGTGTCAGG AAGCCTGCAG CTGGGGGTGG GCAGAAACCA 
ACCAAGCATG AAGAAACCAA GCAGAATGTC AAGACACAGA GGCTGGAGGC AATCAACCAG 
CAGTGGGGCT TTGGCGGAGC GAGAGAAGGA GATGTCGGAG AGGAAGAAGA GCAGGTCACT 
GCAGAGGAAA GAGAGCACAG GTCTAGTAAA AGAGTTTCCT TCCAAAGTCC AGCCACCTTG 
CCTGTCACTG CGGAGCCTCA GAAGGAGGAT CTCTCTCAGG GGAACACCAT AATCCTCAGC 
CCCAGCAGAA GCATCCTGAA GGTGGTTCAG CCAGGAGAGA ACGTCCCACT CTCTGAGGAC 
CAGGACGGTC CAAGCCCACA CACCACCCCT TCTCAGCTGG ATAAGATATC TGCAAAGCAG 
CACTCCAAAC CAAATGAAGA AATGACTAAA AAGCCTGACC TGCCCTCCCC TCCTAAGCGC 
ACTCCCAAAA GATTCTGTGT GGAAGATAAA AACATCTCAC AGTGTACCCC AAAGAAACCC 
AAGGCATCTC CTGGCCAGGG TAGACGTCCG GCAGTGGACA GGGTTGCACT CCCAGCCATG 
CATACCTCGC CCTCCCCCGC TGCCTGCCCC AGACAGGGTC GAACCAGCAC CAGGATATCC 
AGAGAAGGAG AGCAGCAGTG GGGCAGTAGT CCGTGTTCCC CCGCCCCCCT GGGGAAGAGA 
TCCCCGGGCA GACTTTCACA GAACAGCCCA GCGGTAGTGA AGAGAAACCA CAAGGGGGAG 
ACACCCCTAC ACCTGGCAGC AATAAAGGGT GATGTGGAGA CAGTGAAGGA GCTCCTAGAC 
CAAGGGGCTG ACCCCAACCT GAAAGACAAT GCTGGATGGA CACCGCTGCA CGAGGCATGT 
AACCTGGGCC ACCTGGGTGT AGTGGAGGTG TTGGTGGAGA GGGGAGGCGT GCTGTTGAAC 
ACACCAGGCT ACAAGAACGA TTCTCCTCTA CACGACGCTG TCAGGAACGG ACATACTGGC 
ATTGCCATGC TACTGCTGCA GCACGGGGCT TCACAGAGCG TCTTGAATAT GTTTGGCCTT 
CGCCCTGTGG ACTATGCCGT GAGCCCAGAG ATGCAGGCAA TCCTTCTGGC AGCCTCTGAA 
GGACCCCACC CAGTCAACTC ACCTCTCAGT CCTTCACCCA GCCTCAATAA GGCCAGTGAA 
GGTGTGAGAC TGGAGGAACA GGCAGTTGTA CTGGGTAGTA AACTGTCCCA GTCCCAACAG 
ACCCGGCTGG CCAAACTGGG ACATCTCCTG GGAGGAAAGA GGGCAGACGC CTTCTCCTGC 
TCTGTCACTC ATGTGGTGGT ACCAGACGGG CCGATGCCCA CCACCCTGTC CACTCTCCTT 
GGCCTCCTGA ACGGCTGCTG GGTCCTCAAC TTTAGCTGGG TAGAGGCGTG CCTGCAGGCA 
GACAGCTGGA TCTCCGAGAG TGACTATGAA GCAGGAGCAG GACCGCAACG CAGCCGCATT 
AACAGACACA GCCTGCTGCC CCCGCTGTTG GACGGTTGTT TCTTCTACCT CATGGGCTCC 
TTCAGGAAGC CCCCCAGGGA CGAGCTCCTC CAGCTGGTGA GAGAGGCAGG GGGCCAGATT 
CTTACCCGAC AGCCCAAACC AGACAGTGAT GTGACCCAGA CCCTGTCTGC TGCAGCCTAC 
CACGCCTCTC TGGGCTCAGA CCAGACCCTG TGTACCCAGT ACATCATCTA TGATCCCCAG 
GGCTCTTACA GGCCACCCAG GGTGAGGTTA GGAAAGGTGT GGTCAGCTCC ATCTACATGG 
GTTGTAAACT GTATATCGGC CTTCCAGCTA CTGCCAGTGC CTGAGCTTGA ATCAGACACA 
CCTGGTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020317808.1
CDS	257..2728
Translation	MDPDLCTDEGWTKTKEAVAHFRKLLLCSKCSKLMNEPVCLGVCEHMLCRSCAGPQAGEGCVVCHSPAWVKDIRINRQLSNITELYGNLDSLLNPTGSDYSDALPITPPAQSDKSVLKHKKNFKIWFSPRSRKVRCRVEKPAAVPLPEVGSGTVKAAPRHPETLPESKGKDLSVFNFNSSSQDSGSSSPQRATNYEKKKATKKTRNGGGVRKPAAGGGQKPTKHEETKQNVKTQRLEAINQQWGFGGAREGDVGEEEEQVTAEEREHRSSKRVSFQSPATLPVTAEPQKEDLSQGNTIILSPSRSILKVVQPGENVPLSEDQDGPSPHTTPSQLDKISAKQHSKPNEEMTKKPDLPSPPKRTPKRFCVEDKNISQCTPKKPKASPGQGRRPAVDRVALPAMHTSPSPAACPRQGRTSTRISREGEQQWGSSPCSPAPLGKRSPGRLSQNSPAVVKRNHKGETPLHLAAIKGDVETVKELLDQGADPNLKDNAGWTPLHEACNLGHLGVVEVLVERGGVLLNTPGYKNDSPLHDAVRNGHTGIAMLLLQHGASQSVLNMFGLRPVDYAVSPEMQAILLAASEGPHPVNSPLSPSPSLNKASEGVRLEEQAVVLGSKLSQSQQTRLAKLGHLLGGKRADAFSCSVTHVVVPDGPMPTTLSTLLGLLNGCWVLNFSWVEACLQADSWISESDYEAGAGPQRSRINRHSLLPPLLDGCFFYLMGSFRKPPRDELLQLVREAGGQILTRQPKPDSDVTQTLSAAAYHASLGSDQTLCTQYIIYDPQGSYRPPRVRLGKVWSAPSTWVVNCISAFQLLPVPELESDTPGV

Target ORF information:

RefSeq Version	XM_020462219.2
Organism	Oncorhynchus kisutch(coho salmon)
Definition	Oncorhynchus kisutch BRCA1 associated RING domain 1 (bard1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020462219.2

ORF Insert Sequence:

ATGGATCCTG ATCTTTGCAC GGACGAAGGC TGGACGAAAA CGAAGGAGGC TGTCGCGCAT 
TTTCGAAAAC TTTTGTTGTG CTCAAAATGT TCAAAACTGA TGAACGAACC TGTGTGCCTC 
GGGGTTTGTG AACATATGCT TTGCAGGTCG TGTGCTGGTC CTCAGGCTGG AGAGGGATGT 
GTGGTGTGTC ACAGTCCTGC CTGGGTGAAA GACATTCGGA TCAACAGACA ACTCAGCAAT 
ATCACAGAAC TCTATGGCAA CCTAGACTCA CTGCTCAATC CCACTGGCTC CGATTATTCT 
GATGCCCTTC CCATTACACC ACCAGCACAG TCAGATAAAT CTGTCTTAAA GCACAAGAAG 
AACTTCAAGA TCTGGTTTAG TCCCCGCAGC CGCAAGGTGC GCTGCCGGGT AGAGAAGCCT 
GCGGCGGTGC CTCTCCCGGA GGTTGGGAGT GGGACTGTGA AAGCTGCTCC AAGACATCCT 
GAGACACTAC CTGAGTCTAA AGGCAAAGAC CTGTCTGTCT TCAACTTCAA CTCCTCCTCC 
CAAGACTCTG GCTCCTCCTC TCCTCAAAGG GCGACTAACT ATGAAAAGAA GAAGGCGACT 
AAGAAGACGA GAAATGGTGG TGGTGTCAGG AAGCCTGCAG CTGGGGGTGG GCAGAAACCA 
ACCAAGCATG AAGAAACCAA GCAGAATGTC AAGACACAGA GGCTGGAGGC AATCAACCAG 
CAGTGGGGCT TTGGCGGAGC GAGAGAAGGA GATGTCGGAG AGGAAGAAGA GCAGGTCACT 
GCAGAGGAAA GAGAGCACAG GTCTAGTAAA AGAGTTTCCT TCCAAAGTCC AGCCACCTTG 
CCTGTCACTG CGGAGCCTCA GAAGGAGGAT CTCTCTCAGG GGAACACCAT AATCCTCAGC 
CCCAGCAGAA GCATCCTGAA GGTGGTTCAG CCAGGAGAGA ACGTCCCACT CTCTGAGGAC 
CAGGACGGTC CAAGCCCACA CACCACCCCT TCTCAGCTGG ATAAGATATC TGCAAAGCAG 
CACTCCAAAC CAAATGAAGA AATGACTAAA AAGCCTGACC TGCCCTCCCC TCCTAAGCGC 
ACTCCCAAAA GATTCTGTGT GGAAGATAAA AACATCTCAC AGTGTACCCC AAAGAAACCC 
AAGGCATCTC CTGGCCAGGG TAGACGTCCG GCAGTGGACA GGGTTGCACT CCCAGCCATG 
CATACCTCGC CCTCCCCCGC TGCCTGCCCC AGACAGGGTC GAACCAGCAC CAGGATATCC 
AGAGAAGGAG AGCAGCAGTG GGGCAGTAGT CCGTGTTCCC CCGCCCCCCT GGGGAAGAGA 
TCCCCGGGCA GACTTTCACA GAACAGCCCA GCGGTAGTGA AGAGAAACCA CAAGGGGGAG 
ACACCCCTAC ACCTGGCAGC AATAAAGGGT GATGTGGAGA CAGTGAAGGA GCTCCTAGAC 
CAAGGGGCTG ACCCCAACCT GAAAGACAAT GCTGGATGGA CACCGCTGCA CGAGGCATGT 
AACCTGGGCC ACCTGGGTGT AGTGGAGGTG TTGGTGGAGA GGGGAGGCGT GCTGTTGAAC 
ACACCAGGCT ACAAGAACGA TTCTCCTCTA CACGACGCTG TCAGGAACGG ACATACTGGC 
ATTGCCATGC TACTGCTGCA GCACGGGGCT TCACAGAGCG TCTTGAATAT GTTTGGCCTT 
CGCCCTGTGG ACTATGCCGT GAGCCCAGAG ATGCAGGCAA TCCTTCTGGC AGCCTCTGAA 
GGACCCCACC CAGTCAACTC ACCTCTCAGT CCTTCACCCA GCCTCAATAA GGCCAGTGAA 
GGTGTGAGAC TGGAGGAACA GGCAGTTGTA CTGGGTAGTA AACTGTCCCA GTCCCAACAG 
ACCCGGCTGG CCAAACTGGG ACATCTCCTG GGAGGAAAGA GGGCAGACGC CTTCTCCTGC 
TCTGTCACTC ATGTGGTGGT ACCAGACGGG CCGATGCCCA CCACCCTGTC CACTCTCCTT 
GGCCTCCTGA ACGGCTGCTG GGTCCTCAAC TTTAGCTGGG TAGAGGCGTG CCTGCAGGCA 
GACAGCTGGA TCTCCGAGAG TGACTATGAA GCAGGAGCAG GACCGCAACG CAGCCGCATT 
AACAGACACA GCCTGCTGCC CCCGCTGTTG GACGGTTGTT TCTTCTACCT CATGGGCTCC 
TTCAGGAAGC CCCCCAGGGA CGAGCTCCTC CAGCTGGTGA GAGAGGCAGG GGGCCAGATT 
CTTACCCGAC AGCCCAAACC AGACAGTGAT GTGACCCAGA CCCTGTCTGC TGCAGCCTAC 
CACGCCTCTC TGGGCTCAGA CCAGACCCTG TGTACCCAGT ACATCATCTA TGATCCCCAG 
GGCTCTTACA GGCCACCCAG GGTGAGGTTA GGAAAGGTGT GGTCAGCTCC ATCTACATGG 
GTTGTAAACT GTATATCGGC CTTCCAGCTA CTGCCAGTGC CTGAGCTTGA ATCAGACACA 
CCTGGTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bard1 ( XM_020462219.1 ) cDNA ORF clone, Oncorhynchus kisutch(coho salmon) -> XP_020317808.1 Oncorhynchus kisutch BRCA1 associated RING domain 1 (bard1), mRNA.

Price & Availability↑

CloneID	OOn44285
Clone ID Related Accession (Same CDS sequence)	XM_020462219.2 , XM_020462219.1
Accession Version	XM_020462219.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-12
Organism	Oncorhynchus kisutch(coho salmon)
Product	BRCA1-associated RING domain protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034199.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus kisutch Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTG ATCTTTGCAC GGACGAAGGC TGGACGAAAA CGAAGGAGGC TGTCGCGCAT 
TTTCGAAAAC TTTTGTTGTG CTCAAAATGT TCAAAACTGA TGAACGAACC TGTGTGCCTC 
GGGGTTTGTG AACATATGCT TTGCAGGTCG TGTGCTGGTC CTCAGGCTGG AGAGGGATGT 
GTGGTGTGTC ACAGTCCTGC CTGGGTGAAA GACATTCGGA TCAACAGACA ACTCAGCAAT 
ATCACAGAAC TCTATGGCAA CCTAGACTCA CTGCTCAATC CCACTGGCTC CGATTATTCT 
GATGCCCTTC CCATTACACC ACCAGCACAG TCAGATAAAT CTGTCTTAAA GCACAAGAAG 
AACTTCAAGA TCTGGTTTAG TCCCCGCAGC CGCAAGGTGC GCTGCCGGGT AGAGAAGCCT 
GCGGCGGTGC CTCTCCCGGA GGTTGGGAGT GGGACTGTGA AAGCTGCTCC AAGACATCCT 
GAGACACTAC CTGAGTCTAA AGGCAAAGAC CTGTCTGTCT TCAACTTCAA CTCCTCCTCC 
CAAGACTCTG GCTCCTCCTC TCCTCAAAGG GCGACTAACT ATGAAAAGAA GAAGGCGACT 
AAGAAGACGA GAAATGGTGG TGGTGTCAGG AAGCCTGCAG CTGGGGGTGG GCAGAAACCA 
ACCAAGCATG AAGAAACCAA GCAGAATGTC AAGACACAGA GGCTGGAGGC AATCAACCAG 
CAGTGGGGCT TTGGCGGAGC GAGAGAAGGA GATGTCGGAG AGGAAGAAGA GCAGGTCACT 
GCAGAGGAAA GAGAGCACAG GTCTAGTAAA AGAGTTTCCT TCCAAAGTCC AGCCACCTTG 
CCTGTCACTG CGGAGCCTCA GAAGGAGGAT CTCTCTCAGG GGAACACCAT AATCCTCAGC 
CCCAGCAGAA GCATCCTGAA GGTGGTTCAG CCAGGAGAGA ACGTCCCACT CTCTGAGGAC 
CAGGACGGTC CAAGCCCACA CACCACCCCT TCTCAGCTGG ATAAGATATC TGCAAAGCAG 
CACTCCAAAC CAAATGAAGA AATGACTAAA AAGCCTGACC TGCCCTCCCC TCCTAAGCGC 
ACTCCCAAAA GATTCTGTGT GGAAGATAAA AACATCTCAC AGTGTACCCC AAAGAAACCC 
AAGGCATCTC CTGGCCAGGG TAGACGTCCG GCAGTGGACA GGGTTGCACT CCCAGCCATG 
CATACCTCGC CCTCCCCCGC TGCCTGCCCC AGACAGGGTC GAACCAGCAC CAGGATATCC 
AGAGAAGGAG AGCAGCAGTG GGGCAGTAGT CCGTGTTCCC CCGCCCCCCT GGGGAAGAGA 
TCCCCGGGCA GACTTTCACA GAACAGCCCA GCGGTAGTGA AGAGAAACCA CAAGGGGGAG 
ACACCCCTAC ACCTGGCAGC AATAAAGGGT GATGTGGAGA CAGTGAAGGA GCTCCTAGAC 
CAAGGGGCTG ACCCCAACCT GAAAGACAAT GCTGGATGGA CACCGCTGCA CGAGGCATGT 
AACCTGGGCC ACCTGGGTGT AGTGGAGGTG TTGGTGGAGA GGGGAGGCGT GCTGTTGAAC 
ACACCAGGCT ACAAGAACGA TTCTCCTCTA CACGACGCTG TCAGGAACGG ACATACTGGC 
ATTGCCATGC TACTGCTGCA GCACGGGGCT TCACAGAGCG TCTTGAATAT GTTTGGCCTT 
CGCCCTGTGG ACTATGCCGT GAGCCCAGAG ATGCAGGCAA TCCTTCTGGC AGCCTCTGAA 
GGACCCCACC CAGTCAACTC ACCTCTCAGT CCTTCACCCA GCCTCAATAA GGCCAGTGAA 
GGTGTGAGAC TGGAGGAACA GGCAGTTGTA CTGGGTAGTA AACTGTCCCA GTCCCAACAG 
ACCCGGCTGG CCAAACTGGG ACATCTCCTG GGAGGAAAGA GGGCAGACGC CTTCTCCTGC 
TCTGTCACTC ATGTGGTGGT ACCAGACGGG CCGATGCCCA CCACCCTGTC CACTCTCCTT 
GGCCTCCTGA ACGGCTGCTG GGTCCTCAAC TTTAGCTGGG TAGAGGCGTG CCTGCAGGCA 
GACAGCTGGA TCTCCGAGAG TGACTATGAA GCAGGAGCAG GACCGCAACG CAGCCGCATT 
AACAGACACA GCCTGCTGCC CCCGCTGTTG GACGGTTGTT TCTTCTACCT CATGGGCTCC 
TTCAGGAAGC CCCCCAGGGA CGAGCTCCTC CAGCTGGTGA GAGAGGCAGG GGGCCAGATT 
CTTACCCGAC AGCCCAAACC AGACAGTGAT GTGACCCAGA CCCTGTCTGC TGCAGCCTAC 
CACGCCTCTC TGGGCTCAGA CCAGACCCTG TGTACCCAGT ACATCATCTA TGATCCCCAG 
GGCTCTTACA GGCCACCCAG GGTGAGGTTA GGAAAGGTGT GGTCAGCTCC ATCTACATGG 
GTTGTAAACT GTATATCGGC CTTCCAGCTA CTGCCAGTGC CTGAGCTTGA ATCAGACACA 
CCTGGTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020317808.1
CDS	129..2600
Translation	MDPDLCTDEGWTKTKEAVAHFRKLLLCSKCSKLMNEPVCLGVCEHMLCRSCAGPQAGEGCVVCHSPAWVKDIRINRQLSNITELYGNLDSLLNPTGSDYSDALPITPPAQSDKSVLKHKKNFKIWFSPRSRKVRCRVEKPAAVPLPEVGSGTVKAAPRHPETLPESKGKDLSVFNFNSSSQDSGSSSPQRATNYEKKKATKKTRNGGGVRKPAAGGGQKPTKHEETKQNVKTQRLEAINQQWGFGGAREGDVGEEEEQVTAEEREHRSSKRVSFQSPATLPVTAEPQKEDLSQGNTIILSPSRSILKVVQPGENVPLSEDQDGPSPHTTPSQLDKISAKQHSKPNEEMTKKPDLPSPPKRTPKRFCVEDKNISQCTPKKPKASPGQGRRPAVDRVALPAMHTSPSPAACPRQGRTSTRISREGEQQWGSSPCSPAPLGKRSPGRLSQNSPAVVKRNHKGETPLHLAAIKGDVETVKELLDQGADPNLKDNAGWTPLHEACNLGHLGVVEVLVERGGVLLNTPGYKNDSPLHDAVRNGHTGIAMLLLQHGASQSVLNMFGLRPVDYAVSPEMQAILLAASEGPHPVNSPLSPSPSLNKASEGVRLEEQAVVLGSKLSQSQQTRLAKLGHLLGGKRADAFSCSVTHVVVPDGPMPTTLSTLLGLLNGCWVLNFSWVEACLQADSWISESDYEAGAGPQRSRINRHSLLPPLLDGCFFYLMGSFRKPPRDELLQLVREAGGQILTRQPKPDSDVTQTLSAAAYHASLGSDQTLCTQYIIYDPQGSYRPPRVRLGKVWSAPSTWVVNCISAFQLLPVPELESDTPGV

Target ORF information:

RefSeq Version	XM_020462219.1
Organism	Oncorhynchus kisutch(coho salmon)
Definition	Oncorhynchus kisutch BRCA1 associated RING domain 1 (bard1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020462219.1

ORF Insert Sequence:

ATGGATCCTG ATCTTTGCAC GGACGAAGGC TGGACGAAAA CGAAGGAGGC TGTCGCGCAT 
TTTCGAAAAC TTTTGTTGTG CTCAAAATGT TCAAAACTGA TGAACGAACC TGTGTGCCTC 
GGGGTTTGTG AACATATGCT TTGCAGGTCG TGTGCTGGTC CTCAGGCTGG AGAGGGATGT 
GTGGTGTGTC ACAGTCCTGC CTGGGTGAAA GACATTCGGA TCAACAGACA ACTCAGCAAT 
ATCACAGAAC TCTATGGCAA CCTAGACTCA CTGCTCAATC CCACTGGCTC CGATTATTCT 
GATGCCCTTC CCATTACACC ACCAGCACAG TCAGATAAAT CTGTCTTAAA GCACAAGAAG 
AACTTCAAGA TCTGGTTTAG TCCCCGCAGC CGCAAGGTGC GCTGCCGGGT AGAGAAGCCT 
GCGGCGGTGC CTCTCCCGGA GGTTGGGAGT GGGACTGTGA AAGCTGCTCC AAGACATCCT 
GAGACACTAC CTGAGTCTAA AGGCAAAGAC CTGTCTGTCT TCAACTTCAA CTCCTCCTCC 
CAAGACTCTG GCTCCTCCTC TCCTCAAAGG GCGACTAACT ATGAAAAGAA GAAGGCGACT 
AAGAAGACGA GAAATGGTGG TGGTGTCAGG AAGCCTGCAG CTGGGGGTGG GCAGAAACCA 
ACCAAGCATG AAGAAACCAA GCAGAATGTC AAGACACAGA GGCTGGAGGC AATCAACCAG 
CAGTGGGGCT TTGGCGGAGC GAGAGAAGGA GATGTCGGAG AGGAAGAAGA GCAGGTCACT 
GCAGAGGAAA GAGAGCACAG GTCTAGTAAA AGAGTTTCCT TCCAAAGTCC AGCCACCTTG 
CCTGTCACTG CGGAGCCTCA GAAGGAGGAT CTCTCTCAGG GGAACACCAT AATCCTCAGC 
CCCAGCAGAA GCATCCTGAA GGTGGTTCAG CCAGGAGAGA ACGTCCCACT CTCTGAGGAC 
CAGGACGGTC CAAGCCCACA CACCACCCCT TCTCAGCTGG ATAAGATATC TGCAAAGCAG 
CACTCCAAAC CAAATGAAGA AATGACTAAA AAGCCTGACC TGCCCTCCCC TCCTAAGCGC 
ACTCCCAAAA GATTCTGTGT GGAAGATAAA AACATCTCAC AGTGTACCCC AAAGAAACCC 
AAGGCATCTC CTGGCCAGGG TAGACGTCCG GCAGTGGACA GGGTTGCACT CCCAGCCATG 
CATACCTCGC CCTCCCCCGC TGCCTGCCCC AGACAGGGTC GAACCAGCAC CAGGATATCC 
AGAGAAGGAG AGCAGCAGTG GGGCAGTAGT CCGTGTTCCC CCGCCCCCCT GGGGAAGAGA 
TCCCCGGGCA GACTTTCACA GAACAGCCCA GCGGTAGTGA AGAGAAACCA CAAGGGGGAG 
ACACCCCTAC ACCTGGCAGC AATAAAGGGT GATGTGGAGA CAGTGAAGGA GCTCCTAGAC 
CAAGGGGCTG ACCCCAACCT GAAAGACAAT GCTGGATGGA CACCGCTGCA CGAGGCATGT 
AACCTGGGCC ACCTGGGTGT AGTGGAGGTG TTGGTGGAGA GGGGAGGCGT GCTGTTGAAC 
ACACCAGGCT ACAAGAACGA TTCTCCTCTA CACGACGCTG TCAGGAACGG ACATACTGGC 
ATTGCCATGC TACTGCTGCA GCACGGGGCT TCACAGAGCG TCTTGAATAT GTTTGGCCTT 
CGCCCTGTGG ACTATGCCGT GAGCCCAGAG ATGCAGGCAA TCCTTCTGGC AGCCTCTGAA 
GGACCCCACC CAGTCAACTC ACCTCTCAGT CCTTCACCCA GCCTCAATAA GGCCAGTGAA 
GGTGTGAGAC TGGAGGAACA GGCAGTTGTA CTGGGTAGTA AACTGTCCCA GTCCCAACAG 
ACCCGGCTGG CCAAACTGGG ACATCTCCTG GGAGGAAAGA GGGCAGACGC CTTCTCCTGC 
TCTGTCACTC ATGTGGTGGT ACCAGACGGG CCGATGCCCA CCACCCTGTC CACTCTCCTT 
GGCCTCCTGA ACGGCTGCTG GGTCCTCAAC TTTAGCTGGG TAGAGGCGTG CCTGCAGGCA 
GACAGCTGGA TCTCCGAGAG TGACTATGAA GCAGGAGCAG GACCGCAACG CAGCCGCATT 
AACAGACACA GCCTGCTGCC CCCGCTGTTG GACGGTTGTT TCTTCTACCT CATGGGCTCC 
TTCAGGAAGC CCCCCAGGGA CGAGCTCCTC CAGCTGGTGA GAGAGGCAGG GGGCCAGATT 
CTTACCCGAC AGCCCAAACC AGACAGTGAT GTGACCCAGA CCCTGTCTGC TGCAGCCTAC 
CACGCCTCTC TGGGCTCAGA CCAGACCCTG TGTACCCAGT ACATCATCTA TGATCCCCAG 
GGCTCTTACA GGCCACCCAG GGTGAGGTTA GGAAAGGTGT GGTCAGCTCC ATCTACATGG 
GTTGTAAACT GTATATCGGC CTTCCAGCTA CTGCCAGTGC CTGAGCTTGA ATCAGACACA 
CCTGGTGTGT AA