CAST cDNA ORF clone, Ochotona princeps(American pika)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAST
Entrez Gene ID	101517902
Full Name	calpastatin
Gene Type	protein-coding
Organism	Ochotona princeps(American pika)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012927289.1	XP_012782743.1	calpastatin

Pathways from BioSystems
Gene Ontology

The following CAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOc75020	XM_012927289.1 Latest version!	Ochotona princeps calpastatin (CAST), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAST ( XM_012927289.1 ) cDNA ORF clone, Ochotona princeps(American pika) -> XP_012782743.1 Ochotona princeps calpastatin (CAST), mRNA.

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CloneID	OOc75020
Clone ID Related Accession (Same CDS sequence)	XM_012927289.1
Accession Version	XM_012927289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-18
Organism	Ochotona princeps(American pika)
Product	calpastatin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004535459.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ochotona princeps Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCTA CGGGAGCCAA GACTGCAAAA ACAGAGAAGT CACAACCAAC CCAGACGACC 
GATGGTCCCG AGAAAAAACC CCGAGAAGCT AAGCCAAAGG AACAACCCGA GCCAAAAAGC 
TCTTCTAGAC ACACATCCGA GCAAGGAAGC AAGGATGGTT CTAAGGAGAA GGAGCCGTCT 
GCACCAGAGA AAGCAGCAGA GCCAAAGACT AAACCACGGG ACACGGTATC TGCTGGTGAG 
AAGAATGTTG CTGCTGGGAT AACCGCAACA CCTGGAAAAC CAGATGACCA GAAGAAGCAA 
AAGAAACCAT TGGCAGCCGC TGCACCAGCT GAACCAAAGC CGGATAAGCC ATCGGGAAAG 
TCAGGCATGG AAGCGGCTTT GGATGACCTA ATAGACACGT TAGGAGATCC CGGCGAGACA 
AATGAAGATA CTACGCCTTA CACAGGACCA GAAATCTCAG ACCCCATGAT TTCCACCTAC 
CTAGAGGAAC TGGGTAAAAG AGAAGGTACA ATTCCTCCAG AATACAGAGA GCTTCTGGCC 
AAAAAAACGG GGATCACAGT GCCTCCACCA GACTCTTCGG AGTCCATGGG ACCCGATGAT 
GCCATCGACG CCCTGTCGTC CGACTTCACC TGCCCCTCTC CTGCGGCCGG CGCGAAGGAA 
GCCAAGAAAG AGAAATCTGA AGGAGAGGTT TTAAAAGCTG AATCAGCCAA AGTAATCAAA 
AGTGCAGCCC CACCCCAAGA GAAGAAAAGA AAAGTGGAGG AGGATGCCAT GAGTGACCAA 
GCATTGGAGG CCCTGTCGGC TTCCCTGGGC ACCCGGATAC CAGAACCCGA GCTTGACCTC 
GGCTCCATTA AGGAAGTGGC CGAGTCAAAA GCTAAAGAAG AGAAAGTCGA GAAGTGCGGT 
GAGGATGATG AGACTGTACC AGCCGAGTAC AGACTAAAGC CAGCCATGGA TAAAGATGGG 
AAGCCACTGT TACCAGAGCC TGAAGAGAAA CCGAAGGTGC GGAGTGAGTC GGAACTCATC 
GATGAGCTTT CCGAAGGATT CAGCCGCACG AAATGGAAAG ACAAACAGTC GAAGCCAGCT 
GAAAACACAG AGGACTCCAA GGCCGCTGCA TCCGCTCCAG TGACGGAAGC CGTGGGCCGG 
ACCTCCATGT GCAGCGTGCA GTCGGCCCCC GCCAAGCCTG CCTCCCAGAA GGGCCGGGTG 
CCAGATGACG CCGTGGACAC CCTGGCCAGC AGCCTGGGAA GGAAGGAAGC AGACCCAGAG 
GATGGAAAAC CTGTGGCGGA TCAAACCAAG GAGAAAGCCA AGGAAAAAGA CCGAGAAAAG 
CTTGGTGAAA AGGAAGAAAC GATCCCCCCC GATTATAGAT TGGAAGAGGT TAAGGATAAA 
GATGGAAAAC CACTCCCACC AGCAGAGCCC AAGGCAGAGT TTCCAGCCAT GACCGAGGAC 
GATCTACTGG ATGCCTTGTC TGAGGACTTC GGGAGTTTGT CAAAAGCATC GCCACTGAAA 
TTCGACGATG CCAAACTCGC CGCCGCTGTC TCTGAAGTGG TCTCCCACAC ACCAGCGCCC 
GTCACCCACG CTGCAGCCCC GCCCCCTCAC GCACAGCCTA GTGACAAAGA GCTTGACGAT 
GCTTTGGACA AACTTTCTGA CAGTCTTGGG CAAAGGCAGC CTGATCCAGA TGAGAACAAA 
CCAATGGAGG ATAAAGTGAA GGAGAGAGCC AAAAAGGAGC ATAGAGACAA GCTTGGGGAG 
AGAGATGACA CCATCCCCCC CGAGTACAGA CACCTGCTGG ACCAGGGTGA CAAGGACAAA 
CCGGAGAAGC CAACTACAAA GAAATCAAAG GAAATGAAGA AGCCGGCAGG TGAACAGGAC 
CCCATTGATG CCCTCTCATC AGACCTGGAC AGCTGCCCTT CAGCCACAGA GACCTCACAG 
GACGCCAAGA AGGAAAAGAG CAAGAAGACA GTGTCCAGTT CCAAAGCGGG CAAACATGCA 
GACAAAGCAA AGAATTCCAC AAAGACGACA GAGGAAACCT CCAAGCCACA AAAGAATGAA 
AAAACTACGA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012782743.1
CDS	127..2181
Translation	MNPTGAKTAKTEKSQPTQTTDGPEKKPREAKPKEQPEPKSSSRHTSEQGSKDGSKEKEPSAPEKAAEPKTKPRDTVSAGEKNVAAGITATPGKPDDQKKQKKPLAAAAPAEPKPDKPSGKSGMEAALDDLIDTLGDPGETNEDTTPYTGPEISDPMISTYLEELGKREGTIPPEYRELLAKKTGITVPPPDSSESMGPDDAIDALSSDFTCPSPAAGAKEAKKEKSEGEVLKAESAKVIKSAAPPQEKKRKVEEDAMSDQALEALSASLGTRIPEPELDLGSIKEVAESKAKEEKVEKCGEDDETVPAEYRLKPAMDKDGKPLLPEPEEKPKVRSESELIDELSEGFSRTKWKDKQSKPAENTEDSKAAASAPVTEAVGRTSMCSVQSAPAKPASQKGRVPDDAVDTLASSLGRKEADPEDGKPVADQTKEKAKEKDREKLGEKEETIPPDYRLEEVKDKDGKPLPPAEPKAEFPAMTEDDLLDALSEDFGSLSKASPLKFDDAKLAAAVSEVVSHTPAPVTHAAAPPPHAQPSDKELDDALDKLSDSLGQRQPDPDENKPMEDKVKERAKKEHRDKLGERDDTIPPEYRHLLDQGDKDKPEKPTTKKSKEMKKPAGEQDPIDALSSDLDSCPSATETSQDAKKEKSKKTVSSSKAGKHADKAKNSTKTTEETSKPQKNEKTTS

Target ORF information:

RefSeq Version	XM_012927289.1
Organism	Ochotona princeps(American pika)
Definition	Ochotona princeps calpastatin (CAST), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012927289.1

ORF Insert Sequence:

ATGAATCCTA CGGGAGCCAA GACTGCAAAA ACAGAGAAGT CACAACCAAC CCAGACGACC 
GATGGTCCCG AGAAAAAACC CCGAGAAGCT AAGCCAAAGG AACAACCCGA GCCAAAAAGC 
TCTTCTAGAC ACACATCCGA GCAAGGAAGC AAGGATGGTT CTAAGGAGAA GGAGCCGTCT 
GCACCAGAGA AAGCAGCAGA GCCAAAGACT AAACCACGGG ACACGGTATC TGCTGGTGAG 
AAGAATGTTG CTGCTGGGAT AACCGCAACA CCTGGAAAAC CAGATGACCA GAAGAAGCAA 
AAGAAACCAT TGGCAGCCGC TGCACCAGCT GAACCAAAGC CGGATAAGCC ATCGGGAAAG 
TCAGGCATGG AAGCGGCTTT GGATGACCTA ATAGACACGT TAGGAGATCC CGGCGAGACA 
AATGAAGATA CTACGCCTTA CACAGGACCA GAAATCTCAG ACCCCATGAT TTCCACCTAC 
CTAGAGGAAC TGGGTAAAAG AGAAGGTACA ATTCCTCCAG AATACAGAGA GCTTCTGGCC 
AAAAAAACGG GGATCACAGT GCCTCCACCA GACTCTTCGG AGTCCATGGG ACCCGATGAT 
GCCATCGACG CCCTGTCGTC CGACTTCACC TGCCCCTCTC CTGCGGCCGG CGCGAAGGAA 
GCCAAGAAAG AGAAATCTGA AGGAGAGGTT TTAAAAGCTG AATCAGCCAA AGTAATCAAA 
AGTGCAGCCC CACCCCAAGA GAAGAAAAGA AAAGTGGAGG AGGATGCCAT GAGTGACCAA 
GCATTGGAGG CCCTGTCGGC TTCCCTGGGC ACCCGGATAC CAGAACCCGA GCTTGACCTC 
GGCTCCATTA AGGAAGTGGC CGAGTCAAAA GCTAAAGAAG AGAAAGTCGA GAAGTGCGGT 
GAGGATGATG AGACTGTACC AGCCGAGTAC AGACTAAAGC CAGCCATGGA TAAAGATGGG 
AAGCCACTGT TACCAGAGCC TGAAGAGAAA CCGAAGGTGC GGAGTGAGTC GGAACTCATC 
GATGAGCTTT CCGAAGGATT CAGCCGCACG AAATGGAAAG ACAAACAGTC GAAGCCAGCT 
GAAAACACAG AGGACTCCAA GGCCGCTGCA TCCGCTCCAG TGACGGAAGC CGTGGGCCGG 
ACCTCCATGT GCAGCGTGCA GTCGGCCCCC GCCAAGCCTG CCTCCCAGAA GGGCCGGGTG 
CCAGATGACG CCGTGGACAC CCTGGCCAGC AGCCTGGGAA GGAAGGAAGC AGACCCAGAG 
GATGGAAAAC CTGTGGCGGA TCAAACCAAG GAGAAAGCCA AGGAAAAAGA CCGAGAAAAG 
CTTGGTGAAA AGGAAGAAAC GATCCCCCCC GATTATAGAT TGGAAGAGGT TAAGGATAAA 
GATGGAAAAC CACTCCCACC AGCAGAGCCC AAGGCAGAGT TTCCAGCCAT GACCGAGGAC 
GATCTACTGG ATGCCTTGTC TGAGGACTTC GGGAGTTTGT CAAAAGCATC GCCACTGAAA 
TTCGACGATG CCAAACTCGC CGCCGCTGTC TCTGAAGTGG TCTCCCACAC ACCAGCGCCC 
GTCACCCACG CTGCAGCCCC GCCCCCTCAC GCACAGCCTA GTGACAAAGA GCTTGACGAT 
GCTTTGGACA AACTTTCTGA CAGTCTTGGG CAAAGGCAGC CTGATCCAGA TGAGAACAAA 
CCAATGGAGG ATAAAGTGAA GGAGAGAGCC AAAAAGGAGC ATAGAGACAA GCTTGGGGAG 
AGAGATGACA CCATCCCCCC CGAGTACAGA CACCTGCTGG ACCAGGGTGA CAAGGACAAA 
CCGGAGAAGC CAACTACAAA GAAATCAAAG GAAATGAAGA AGCCGGCAGG TGAACAGGAC 
CCCATTGATG CCCTCTCATC AGACCTGGAC AGCTGCCCTT CAGCCACAGA GACCTCACAG 
GACGCCAAGA AGGAAAAGAG CAAGAAGACA GTGTCCAGTT CCAAAGCGGG CAAACATGCA 
GACAAAGCAA AGAATTCCAC AAAGACGACA GAGGAAACCT CCAAGCCACA AAAGAATGAA 
AAAACTACGA GTTAA