WLS cDNA ORF clone, Numida meleagris(helmeted guineafowl)

Gene
Clones
gRNAs

Summary

Gene Symbol	WLS
Entrez Gene ID	110402665
Full Name	wntless Wnt ligand secretion mediator
Gene Type	protein-coding
Organism	Numida meleagris(helmeted guineafowl)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021405021.1	XP_021260696.1	protein wntless homolog

Pathways from BioSystems
Gene Ontology

The following WLS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WLS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONu28947	XM_021405021.1 Latest version!	Numida meleagris wntless Wnt ligand secretion mediator (WLS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WLS ( XM_021405021.1 ) cDNA ORF clone, Numida meleagris(helmeted guineafowl) -> XP_021260696.1 Numida meleagris wntless Wnt ligand secretion mediator (WLS), mRNA.

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CloneID	ONu28947
Clone ID Related Accession (Same CDS sequence)	XM_021405021.1
Accession Version	XM_021405021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-06
Organism	Numida meleagris(helmeted guineafowl)
Product	protein wntless homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034415.1) and transcript sequence (GBYG01017971.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Numida meleagris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 186 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCCGGGG CCATCATCGA GAACATGAGC ACCCGCAAGC TGTGCATCGT CGGCGGGATC 
CTGCTGGTGT TCCAAGTGAT CGCCTTTCTG GTGGGAGGTC TGATCGCTCC CAGCCCCACT 
ACAGCTGTTC CTTACATGTC AGTGAAGTGC ATTGATGTAA GAAAAAACCA CCACAAGACC 
AAGTGGCTGA TGCCCTGGGG ACCCAACCAC TGCGAGAAGC TCAAGGACTT TGACGAGGCG 
GTCAGCAGGC AGATCGAGGC CAATGACATT GTCTTTGCTG TGCACATCCC TCTCCCCAGC 
AAGGAGATGA GCCCCTGGTT CCAGTTCATG CTCTTCATCA TGCAGCTGGA CATCGCCTTC 
AAGATGGACA ATGACCTGAA AGAAAATGCA GAAATTACCC TGGATGTGTC CTTAGCATAT 
CGTGATGACA TGTTTGGTGA CTGGGAAGAA ATAGCACACG CAATAGAGAT CAGGAAGCTG 
AAATGCACCT TTGGTTCCCC AAAAACCCTG GAGTCTGAAG GCCGTCACTA CGACTGCGAC 
TTCCTTCCCT TCATGGAGAT CGGCAGCGTG GCTCACAAGT ACTACCTCAT CAACATCCGC 
CTGCCCGTGA ACGAGAGGAA GGGCATTAAT GTGGGCATCG GGGAAATCAA GGACATCCGC 
CTGGTGGGTA TCCACCAAAA CGGAGGCTTT ACGAAAGTGT GGTTTGCCAT GAAGACCTTC 
CTCACCCCCA GCATTTTGAT CATCATGGTC TGGTACTGGA GACGGATCAC GCTGATGACA 
CGGGCTCCTG TCCTGCTGGA GAAGGTCATC TTTGCTCTGG GAATTTCCAT GACGTTCATT 
AACATCCCAG TGGAGTGGTT TTCCATCGGG TTTGACTGGA CCTGGATGTT GCTCTTTGGA 
GACATTCGAC AAGGCATTTT CTACGCCATG CTGCTGTCAT TTTGGATCAT CTTCTGTGGA 
GAGCACATGA TGGACCAGAA CGAGCGGAAC CGCCTCTCGG GGTACTGGAA GCAGGTTGGA 
CCCATAGCTG TTGGCTCCTT CTGCCTCTTC ATCTTCGACA TGTGTGAGAG GGGAGTGCAG 
CTGAAAAACC CCTTCTACAG CATCTGGACC ACCGAGGTTG GCACGGAGCT GGCTATGGCC 
TTTATTATAG TTGCGGGAAT TTGCTTGTGC CTCTATTTTC TCTTCCTGTG TTTTATGGTC 
TTCCAAGTGT TCAGAAACAT CAGTGGAAAG CAATCCAGCC TGCCAGCGAT GAGCAAGGCC 
CGCCGCCTGC ATTACGAGGG GCTGATCTTT AGGTTCAAGT TCCTGATGCT CATCACCCTG 
GCTTGCGCAG CAATGACAGT CATTTTCTTC ATCGTCAGCC AGGTGACAGA AGGCCACTGG 
AAGTGGGGGG ACATCACGAT ACAAGTGAAC AGTGCCTTCT TCACCGGCAT CTACGGGATG 
TGGAACCTCT ACGTGTTTGC ACTCATGTTC CTGTATGCGC CGTCGCACAA GAACTACGGG 
GAAGATCAGT CGAATGGTGA CCTGGGGGTG AGCAGCGGGG AGGAGCTCCA GCTCACCACC 
ACCATCACCC ACGTGGACGG CCCCACGGAG GTTTACAAGC TGGCTCGCAA GGAGGCTCAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021260696.1
CDS	81..1706
Translation	MAGAIIENMSTRKLCIVGGILLVFQVIAFLVGGLIAPSPTTAVPYMSVKCIDVRKNHHKTKWLMPWGPNHCEKLKDFDEAVSRQIEANDIVFAVHIPLPSKEMSPWFQFMLFIMQLDIAFKMDNDLKENAEITLDVSLAYRDDMFGDWEEIAHAIEIRKLKCTFGSPKTLESEGRHYDCDFLPFMEIGSVAHKYYLINIRLPVNERKGINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSILIIMVWYWRRITLMTRAPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQNERNRLSGYWKQVGPIAVGSFCLFIFDMCERGVQLKNPFYSIWTTEVGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKARRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGDITIQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVSSGEELQLTTTITHVDGPTEVYKLARKEAQE

Target ORF information:

RefSeq Version	XM_021405021.1
Organism	Numida meleagris(helmeted guineafowl)
Definition	Numida meleagris wntless Wnt ligand secretion mediator (WLS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021405021.1

ORF Insert Sequence:

ATGGCCGGGG CCATCATCGA GAACATGAGC ACCCGCAAGC TGTGCATCGT CGGCGGGATC 
CTGCTGGTGT TCCAAGTGAT CGCCTTTCTG GTGGGAGGTC TGATCGCTCC CAGCCCCACT 
ACAGCTGTTC CTTACATGTC AGTGAAGTGC ATTGATGTAA GAAAAAACCA CCACAAGACC 
AAGTGGCTGA TGCCCTGGGG ACCCAACCAC TGCGAGAAGC TCAAGGACTT TGACGAGGCG 
GTCAGCAGGC AGATCGAGGC CAATGACATT GTCTTTGCTG TGCACATCCC TCTCCCCAGC 
AAGGAGATGA GCCCCTGGTT CCAGTTCATG CTCTTCATCA TGCAGCTGGA CATCGCCTTC 
AAGATGGACA ATGACCTGAA AGAAAATGCA GAAATTACCC TGGATGTGTC CTTAGCATAT 
CGTGATGACA TGTTTGGTGA CTGGGAAGAA ATAGCACACG CAATAGAGAT CAGGAAGCTG 
AAATGCACCT TTGGTTCCCC AAAAACCCTG GAGTCTGAAG GCCGTCACTA CGACTGCGAC 
TTCCTTCCCT TCATGGAGAT CGGCAGCGTG GCTCACAAGT ACTACCTCAT CAACATCCGC 
CTGCCCGTGA ACGAGAGGAA GGGCATTAAT GTGGGCATCG GGGAAATCAA GGACATCCGC 
CTGGTGGGTA TCCACCAAAA CGGAGGCTTT ACGAAAGTGT GGTTTGCCAT GAAGACCTTC 
CTCACCCCCA GCATTTTGAT CATCATGGTC TGGTACTGGA GACGGATCAC GCTGATGACA 
CGGGCTCCTG TCCTGCTGGA GAAGGTCATC TTTGCTCTGG GAATTTCCAT GACGTTCATT 
AACATCCCAG TGGAGTGGTT TTCCATCGGG TTTGACTGGA CCTGGATGTT GCTCTTTGGA 
GACATTCGAC AAGGCATTTT CTACGCCATG CTGCTGTCAT TTTGGATCAT CTTCTGTGGA 
GAGCACATGA TGGACCAGAA CGAGCGGAAC CGCCTCTCGG GGTACTGGAA GCAGGTTGGA 
CCCATAGCTG TTGGCTCCTT CTGCCTCTTC ATCTTCGACA TGTGTGAGAG GGGAGTGCAG 
CTGAAAAACC CCTTCTACAG CATCTGGACC ACCGAGGTTG GCACGGAGCT GGCTATGGCC 
TTTATTATAG TTGCGGGAAT TTGCTTGTGC CTCTATTTTC TCTTCCTGTG TTTTATGGTC 
TTCCAAGTGT TCAGAAACAT CAGTGGAAAG CAATCCAGCC TGCCAGCGAT GAGCAAGGCC 
CGCCGCCTGC ATTACGAGGG GCTGATCTTT AGGTTCAAGT TCCTGATGCT CATCACCCTG 
GCTTGCGCAG CAATGACAGT CATTTTCTTC ATCGTCAGCC AGGTGACAGA AGGCCACTGG 
AAGTGGGGGG ACATCACGAT ACAAGTGAAC AGTGCCTTCT TCACCGGCAT CTACGGGATG 
TGGAACCTCT ACGTGTTTGC ACTCATGTTC CTGTATGCGC CGTCGCACAA GAACTACGGG 
GAAGATCAGT CGAATGGTGA CCTGGGGGTG AGCAGCGGGG AGGAGCTCCA GCTCACCACC 
ACCATCACCC ACGTGGACGG CCCCACGGAG GTTTACAAGC TGGCTCGCAA GGAGGCTCAG 
GAGTGA