ap4e1 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ap4e1
Entrez Gene ID	107388637
Full Name	adaptor related protein complex 4 epsilon 1 subunit
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015964242.1	XP_015819728.1	AP-4 complex subunit epsilon-1 isoform X2
XM_015964241.1	XP_015819727.1	AP-4 complex subunit epsilon-1 isoform X1

Pathways from BioSystems
Gene Ontology

The following ap4e1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ap4e1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo20654	XM_015964241.1 Latest version!	Nothobranchius furzeri adaptor related protein complex 4 epsilon 1 subunit (ap4e1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ONo20655	XM_015964242.1 Latest version!	Nothobranchius furzeri adaptor related protein complex 4 epsilon 1 subunit (ap4e1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ap4e1 ( XM_015964241.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015819727.1 Nothobranchius furzeri adaptor related protein complex 4 epsilon 1 subunit (ap4e1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONo20654
Clone ID Related Accession (Same CDS sequence)	XM_015964241.1
Accession Version	XM_015964241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	AP-4 complex subunit epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443579.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGATG TTGTGGAGAA GACGCTGACA GCTCTGCCAA GCCTCCTCTC TTTAGACTCT 
CAGCCTGGCT CAGCGAAACT ATCCACCAAC TCCAAGCTAG GAAGCCTCAT TAGAGGAATT 
ACTGAACTAA CATCTAAACA TGAGGAGGAG AAACTAATTC AGCGTGAGCT ATCATCAATT 
AAAGAGCAGG TGTCCTCCCC CAACACCACC ATGAGGCAGA TGAAGGAGCT CATGGTGAGA 
ACCATCTACT GTGAGATGTT GGGCTACGAG GCAACGTTCA GTTATATTCA TGCCATTAAA 
CTGGCCCAGC AAGGGAATGT CCTGGAGAAG AGAGTTGGTT ATCTTGCTGT TTCCTTGTTT 
TTGAATGAAA GCCACGAGTT GCTGCTTCTC CTGGTAAACA CAGTGCTAAA GGATCTCCAG 
AGTACAAACC TGATTGAAGT GTGCATGGCT CTCACTGTTG TCAGCCAGAT GTTCCCTAAA 
GACATGATTC CTGCCATTCT TCCTCTGGTG GAGGAAAAGC TCAACCACTC CAAGGAAATC 
ATTCGACGAA AAGCCGTCCT GGCGTTGTAT AAATTCTACC TGATTGCCCC AAATCAAGTT 
CAGCACATTC ACAACAGGTT TCGTAAAGCG CTGTGTGACA AAGACCCCGG TGTGATGTCG 
GCTTCTTTAC ACATCTACCT ACAGCTGATT CAGGAGAATC CAGAAAGTTA CAAGGACCTC 
ACCGCAAGTT TTGTCACAAT CCTCAAGCAA GTGGTGGCCG GAAAACTTCC CATGGATTTC 
AACTATCACA GTGTTCCTGC TCCATGGCTT CAAATCCAGC TTCTCAGAAT TCTCTCCCTA 
CTTGGGAAAA GTGACCAGAG CACAAGTGAG GTCATGTATG AAATCCTGGA TGAGTCTTTA 
CGGAGAGCAG AGATGAATCA CAACATCACC TATGCTATTT TATACGAGTG TGTAAAATGC 
ATTTACACAA TTCATCCTAA ATCTGAGCTT TTGGAAAAAG CTGCGAAGGT CATCGGCAAT 
TTTGTTCTGT CACAGAGGAT AAATCTAAAG TATCTGGGCC TGAAGGCTCT CACCTATGTG 
GTCCATCAGG ATCCCAGACT GGCCTTGCAG CATCAGATGA CCATTATCGA GTGTCTCGAT 
CACCCCGATG TCATCATAAA GCGAGAGACG CTGGAGCTAC TCTTTCGGAT AACTAACGAC 
CAGAATGTAA CAGTAATCGT AGAGAAGATG CTGGACTTTA TGAGCATCAA TAAGGAGGAT 
CACACCACCG CAGACCTTGT GGGGAAAGTG GCAGAACTGG CAGAAAAATA TGCACCAGAC 
AACGAGTGGT TTGTTGAGAC CATGAACACG GTGTTCTCTG TAGGCGGAGA CACGTTGCAG 
CCCGACATCC CAAACAGTTT CCTTAAACTG CTCTCAGAAG GATTTGATGA TGAGGAGGAG 
AACAAGAAGT TGAGGCTGTT TGCGGTGAAC TCCTACATTT CTCTGCTTCA GGGAGAGCCC 
AGCAAGCTGC CCCAGCGCTT CCTCCAGGCC ATCTGCTGGG TTCTTGGAGA ATATTCTCAT 
TTACGGGAGG ATGTTGAGCC CGATCTGGTC TTGAGGCTGT TGGTTAAACT GTTGAATATA 
AAGAGCACCA GCAGTGAGAC TAAAAGCTGG ATCCTCATGG CGATGACCAA ACTCTGCTCG 
GGTGGAGCAC CTCTTTCCGT GCCTCTGGAG GTTTCTGAAA CATACAGCAG TTCCATGGAC 
ACAATGCTGC GACAAAGAGC TCAGGAGCTG CAGTACCTCA GCCAAAACCC TGAGCTACAG 
GCCAGGGTGC TACCTCGGGG TGTCAGCCAG GAGCCCATGG AGGTAGATTC ATCGCTGTCG 
TTTCTGGATG GATTTGTGTC AGAAGCGCTA GCTGCAGGCG CCGCTCCCTA TAAGCCTCCC 
CATCAAAGAC AGGAGGAACT GTCCCGATCC AAAGCACTGA GTTTGGAGCC CTATGGCCTG 
TCCCTGCCTA TCAGCATGTC CTCTTGCAGC ATCGCAGACA GACAGTCCCC TACGCTGCTG 
TCCGTGAGCT CTGGTCTGTC AGGCGACCGC ACGGATCACT CACACAAAGG CGGCTCCACA 
ACCTTGAAGC TGGATGGAGT GAGGAGGGTC TGGGGGAAAG AAGGTTATCT GGCTCAGAGA 
GAATCTGCAG AGGAAACTGC TCCGGTCGAG GCTCCCAGTC CGCTCCAGCC TCTCGTTCAG 
CAGGGTGACG GCGACGCCTC CTACAGCCAG ACTCCTACAC CAATCCCCGA ATGTGAACAG 
GAAAAACAGC AGCTGGCATC CTCACTGTTC GTTGGCCTGG CCTCCCAGAG CTCCGTGTGT 
CTGATGGGAA AATCTGAGCA AACATCTCAA CGATTCAGAA GAAAACTCAA AGATCCAGCT 
GCATCATTCC CCTCCAGCAC GCAGAACCCA CTGGTTTCTC CTCCCAGTAG TGTGGGTCAC 
CTGCTGCTGG ACTCCAGCAT TTCCTCAGAT CTAATTTCTT CACCTGAACA GACTCATCTA 
AGCTCTGTTG ACCTCACTGT TGCTAATGGA GAAGTAGAGT TACCTGACAG AGACATGAAG 
GGAATAAAAA GTACTCTACT TAGAGAAGAC TCCGCATTAG CAGCCGGTTC TTCTCCACAC 
GAAGAGCTAG ACAGACCTAA AGACTCGTGT CTCACTTCAC ATCTTCCAGC AGAACTTTCT 
GGACTCCCTC ACTCAGAAAT CACTCCCCTA TGCACCAGCC CAAGCCTGGA TCTGTCTTCC 
TGTCATGTTC AAAAAGACGA TTCTTTAGTG CTTCTCGTCT TCATGAAGAG CTCGTCTGAT 
TATGACATAC AGCAGATTCA AGTTCAAGTC ACCTGTGAGG AACTTGAGGT GTCCTGTGTG 
TGTGACAGTC CAGTAGAGGA GTTAAAAAGC CACGGCGTAG TGGTGTGTCA GTATTTTCTA 
ACGGTGAAGA GTCCCTCGGT TTACTTTGTG CTGTCTGGGA TGATGTCGCA CCAGATTCCT 
GATAATTCGC TTCAGGAAAC ACAATTCTCA CACAGAATTC CTTTAACCAG CTTCCTCAGA 
CCTTTGAGAC TTTCCACTGA GGAGTACGGG ACAATGTGGC TGGCCTTTTC ACACGACACC 
AAGCAGAATC TGCCGCTGAT GATGGACGCG GAGGAGCCGC TCACCGCTAC GCTGAAGGTG 
CTGAAGGACA AGCTTCAGCT TCATGTTGTA GAGATTATAG GAAACGAAGG GATTGTAGCT 
TGCTGCCTCA GACAGGATCA GCCGTGCCTC CTGCACTGTC GCGTTCACGC TGGCATGCTG 
GCGGTGTGGC TGCGCTCCCC CATCCCCGAG TTGCCCGACT GCCTCCTCTA CCACTGTCAG 
AGAGCGCTAC AGGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015819727.1
CDS	182..3562
Translation	MSDVVEKTLTALPSLLSLDSQPGSAKLSTNSKLGSLIRGITELTSKHEEEKLIQRELSSIKEQVSSPNTTMRQMKELMVRTIYCEMLGYEATFSYIHAIKLAQQGNVLEKRVGYLAVSLFLNESHELLLLLVNTVLKDLQSTNLIEVCMALTVVSQMFPKDMIPAILPLVEEKLNHSKEIIRRKAVLALYKFYLIAPNQVQHIHNRFRKALCDKDPGVMSASLHIYLQLIQENPESYKDLTASFVTILKQVVAGKLPMDFNYHSVPAPWLQIQLLRILSLLGKSDQSTSEVMYEILDESLRRAEMNHNITYAILYECVKCIYTIHPKSELLEKAAKVIGNFVLSQRINLKYLGLKALTYVVHQDPRLALQHQMTIIECLDHPDVIIKRETLELLFRITNDQNVTVIVEKMLDFMSINKEDHTTADLVGKVAELAEKYAPDNEWFVETMNTVFSVGGDTLQPDIPNSFLKLLSEGFDDEEENKKLRLFAVNSYISLLQGEPSKLPQRFLQAICWVLGEYSHLREDVEPDLVLRLLVKLLNIKSTSSETKSWILMAMTKLCSGGAPLSVPLEVSETYSSSMDTMLRQRAQELQYLSQNPELQARVLPRGVSQEPMEVDSSLSFLDGFVSEALAAGAAPYKPPHQRQEELSRSKALSLEPYGLSLPISMSSCSIADRQSPTLLSVSSGLSGDRTDHSHKGGSTTLKLDGVRRVWGKEGYLAQRESAEETAPVEAPSPLQPLVQQGDGDASYSQTPTPIPECEQEKQQLASSLFVGLASQSSVCLMGKSEQTSQRFRRKLKDPAASFPSSTQNPLVSPPSSVGHLLLDSSISSDLISSPEQTHLSSVDLTVANGEVELPDRDMKGIKSTLLREDSALAAGSSPHEELDRPKDSCLTSHLPAELSGLPHSEITPLCTSPSLDLSSCHVQKDDSLVLLVFMKSSSDYDIQQIQVQVTCEELEVSCVCDSPVEELKSHGVVVCQYFLTVKSPSVYFVLSGMMSHQIPDNSLQETQFSHRIPLTSFLRPLRLSTEEYGTMWLAFSHDTKQNLPLMMDAEEPLTATLKVLKDKLQLHVVEIIGNEGIVACCLRQDQPCLLHCRVHAGMLAVWLRSPIPELPDCLLYHCQRALQES

Target ORF information:

RefSeq Version	XM_015964241.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri adaptor related protein complex 4 epsilon 1 subunit (ap4e1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015964241.1

ORF Insert Sequence:

ATGAGCGATG TTGTGGAGAA GACGCTGACA GCTCTGCCAA GCCTCCTCTC TTTAGACTCT 
CAGCCTGGCT CAGCGAAACT ATCCACCAAC TCCAAGCTAG GAAGCCTCAT TAGAGGAATT 
ACTGAACTAA CATCTAAACA TGAGGAGGAG AAACTAATTC AGCGTGAGCT ATCATCAATT 
AAAGAGCAGG TGTCCTCCCC CAACACCACC ATGAGGCAGA TGAAGGAGCT CATGGTGAGA 
ACCATCTACT GTGAGATGTT GGGCTACGAG GCAACGTTCA GTTATATTCA TGCCATTAAA 
CTGGCCCAGC AAGGGAATGT CCTGGAGAAG AGAGTTGGTT ATCTTGCTGT TTCCTTGTTT 
TTGAATGAAA GCCACGAGTT GCTGCTTCTC CTGGTAAACA CAGTGCTAAA GGATCTCCAG 
AGTACAAACC TGATTGAAGT GTGCATGGCT CTCACTGTTG TCAGCCAGAT GTTCCCTAAA 
GACATGATTC CTGCCATTCT TCCTCTGGTG GAGGAAAAGC TCAACCACTC CAAGGAAATC 
ATTCGACGAA AAGCCGTCCT GGCGTTGTAT AAATTCTACC TGATTGCCCC AAATCAAGTT 
CAGCACATTC ACAACAGGTT TCGTAAAGCG CTGTGTGACA AAGACCCCGG TGTGATGTCG 
GCTTCTTTAC ACATCTACCT ACAGCTGATT CAGGAGAATC CAGAAAGTTA CAAGGACCTC 
ACCGCAAGTT TTGTCACAAT CCTCAAGCAA GTGGTGGCCG GAAAACTTCC CATGGATTTC 
AACTATCACA GTGTTCCTGC TCCATGGCTT CAAATCCAGC TTCTCAGAAT TCTCTCCCTA 
CTTGGGAAAA GTGACCAGAG CACAAGTGAG GTCATGTATG AAATCCTGGA TGAGTCTTTA 
CGGAGAGCAG AGATGAATCA CAACATCACC TATGCTATTT TATACGAGTG TGTAAAATGC 
ATTTACACAA TTCATCCTAA ATCTGAGCTT TTGGAAAAAG CTGCGAAGGT CATCGGCAAT 
TTTGTTCTGT CACAGAGGAT AAATCTAAAG TATCTGGGCC TGAAGGCTCT CACCTATGTG 
GTCCATCAGG ATCCCAGACT GGCCTTGCAG CATCAGATGA CCATTATCGA GTGTCTCGAT 
CACCCCGATG TCATCATAAA GCGAGAGACG CTGGAGCTAC TCTTTCGGAT AACTAACGAC 
CAGAATGTAA CAGTAATCGT AGAGAAGATG CTGGACTTTA TGAGCATCAA TAAGGAGGAT 
CACACCACCG CAGACCTTGT GGGGAAAGTG GCAGAACTGG CAGAAAAATA TGCACCAGAC 
AACGAGTGGT TTGTTGAGAC CATGAACACG GTGTTCTCTG TAGGCGGAGA CACGTTGCAG 
CCCGACATCC CAAACAGTTT CCTTAAACTG CTCTCAGAAG GATTTGATGA TGAGGAGGAG 
AACAAGAAGT TGAGGCTGTT TGCGGTGAAC TCCTACATTT CTCTGCTTCA GGGAGAGCCC 
AGCAAGCTGC CCCAGCGCTT CCTCCAGGCC ATCTGCTGGG TTCTTGGAGA ATATTCTCAT 
TTACGGGAGG ATGTTGAGCC CGATCTGGTC TTGAGGCTGT TGGTTAAACT GTTGAATATA 
AAGAGCACCA GCAGTGAGAC TAAAAGCTGG ATCCTCATGG CGATGACCAA ACTCTGCTCG 
GGTGGAGCAC CTCTTTCCGT GCCTCTGGAG GTTTCTGAAA CATACAGCAG TTCCATGGAC 
ACAATGCTGC GACAAAGAGC TCAGGAGCTG CAGTACCTCA GCCAAAACCC TGAGCTACAG 
GCCAGGGTGC TACCTCGGGG TGTCAGCCAG GAGCCCATGG AGGTAGATTC ATCGCTGTCG 
TTTCTGGATG GATTTGTGTC AGAAGCGCTA GCTGCAGGCG CCGCTCCCTA TAAGCCTCCC 
CATCAAAGAC AGGAGGAACT GTCCCGATCC AAAGCACTGA GTTTGGAGCC CTATGGCCTG 
TCCCTGCCTA TCAGCATGTC CTCTTGCAGC ATCGCAGACA GACAGTCCCC TACGCTGCTG 
TCCGTGAGCT CTGGTCTGTC AGGCGACCGC ACGGATCACT CACACAAAGG CGGCTCCACA 
ACCTTGAAGC TGGATGGAGT GAGGAGGGTC TGGGGGAAAG AAGGTTATCT GGCTCAGAGA 
GAATCTGCAG AGGAAACTGC TCCGGTCGAG GCTCCCAGTC CGCTCCAGCC TCTCGTTCAG 
CAGGGTGACG GCGACGCCTC CTACAGCCAG ACTCCTACAC CAATCCCCGA ATGTGAACAG 
GAAAAACAGC AGCTGGCATC CTCACTGTTC GTTGGCCTGG CCTCCCAGAG CTCCGTGTGT 
CTGATGGGAA AATCTGAGCA AACATCTCAA CGATTCAGAA GAAAACTCAA AGATCCAGCT 
GCATCATTCC CCTCCAGCAC GCAGAACCCA CTGGTTTCTC CTCCCAGTAG TGTGGGTCAC 
CTGCTGCTGG ACTCCAGCAT TTCCTCAGAT CTAATTTCTT CACCTGAACA GACTCATCTA 
AGCTCTGTTG ACCTCACTGT TGCTAATGGA GAAGTAGAGT TACCTGACAG AGACATGAAG 
GGAATAAAAA GTACTCTACT TAGAGAAGAC TCCGCATTAG CAGCCGGTTC TTCTCCACAC 
GAAGAGCTAG ACAGACCTAA AGACTCGTGT CTCACTTCAC ATCTTCCAGC AGAACTTTCT 
GGACTCCCTC ACTCAGAAAT CACTCCCCTA TGCACCAGCC CAAGCCTGGA TCTGTCTTCC 
TGTCATGTTC AAAAAGACGA TTCTTTAGTG CTTCTCGTCT TCATGAAGAG CTCGTCTGAT 
TATGACATAC AGCAGATTCA AGTTCAAGTC ACCTGTGAGG AACTTGAGGT GTCCTGTGTG 
TGTGACAGTC CAGTAGAGGA GTTAAAAAGC CACGGCGTAG TGGTGTGTCA GTATTTTCTA 
ACGGTGAAGA GTCCCTCGGT TTACTTTGTG CTGTCTGGGA TGATGTCGCA CCAGATTCCT 
GATAATTCGC TTCAGGAAAC ACAATTCTCA CACAGAATTC CTTTAACCAG CTTCCTCAGA 
CCTTTGAGAC TTTCCACTGA GGAGTACGGG ACAATGTGGC TGGCCTTTTC ACACGACACC 
AAGCAGAATC TGCCGCTGAT GATGGACGCG GAGGAGCCGC TCACCGCTAC GCTGAAGGTG 
CTGAAGGACA AGCTTCAGCT TCATGTTGTA GAGATTATAG GAAACGAAGG GATTGTAGCT 
TGCTGCCTCA GACAGGATCA GCCGTGCCTC CTGCACTGTC GCGTTCACGC TGGCATGCTG 
GCGGTGTGGC TGCGCTCCCC CATCCCCGAG TTGCCCGACT GCCTCCTCTA CCACTGTCAG 
AGAGCGCTAC AGGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ap4e1 ( XM_015964242.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015819728.1 Nothobranchius furzeri adaptor related protein complex 4 epsilon 1 subunit (ap4e1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONo20655
Clone ID Related Accession (Same CDS sequence)	XM_015964242.1
Accession Version	XM_015964242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	AP-4 complex subunit epsilon-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443579.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCAGA TGAAGGAGCT CATGGTGAGA ACCATCTACT GTGAGATGTT GGGCTACGAG 
GCAACGTTCA GTTATATTCA TGCCATTAAA CTGGCCCAGC AAGGGAATGT CCTGGAGAAG 
AGAGTTGGTT ATCTTGCTGT TTCCTTGTTT TTGAATGAAA GCCACGAGTT GCTGCTTCTC 
CTGGTAAACA CAGTGCTAAA GGATCTCCAG AGTACAAACC TGATTGAAGT GTGCATGGCT 
CTCACTGTTG TCAGCCAGAT GTTCCCTAAA GACATGATTC CTGCCATTCT TCCTCTGGTG 
GAGGAAAAGC TCAACCACTC CAAGGAAATC ATTCGACGAA AAGCCGTCCT GGCGTTGTAT 
AAATTCTACC TGATTGCCCC AAATCAAGTT CAGCACATTC ACAACAGGTT TCGTAAAGCG 
CTGTGTGACA AAGACCCCGG TGTGATGTCG GCTTCTTTAC ACATCTACCT ACAGCTGATT 
CAGGAGAATC CAGAAAGTTA CAAGGACCTC ACCGCAAGTT TTGTCACAAT CCTCAAGCAA 
GTGGTGGCCG GAAAACTTCC CATGGATTTC AACTATCACA GTGTTCCTGC TCCATGGCTT 
CAAATCCAGC TTCTCAGAAT TCTCTCCCTA CTTGGGAAAA GTGACCAGAG CACAAGTGAG 
GTCATGTATG AAATCCTGGA TGAGTCTTTA CGGAGAGCAG AGATGAATCA CAACATCACC 
TATGCTATTT TATACGAGTG TGTAAAATGC ATTTACACAA TTCATCCTAA ATCTGAGCTT 
TTGGAAAAAG CTGCGAAGGT CATCGGCAAT TTTGTTCTGT CACAGAGGAT AAATCTAAAG 
TATCTGGGCC TGAAGGCTCT CACCTATGTG GTCCATCAGG ATCCCAGACT GGCCTTGCAG 
CATCAGATGA CCATTATCGA GTGTCTCGAT CACCCCGATG TCATCATAAA GCGAGAGACG 
CTGGAGCTAC TCTTTCGGAT AACTAACGAC CAGAATGTAA CAGTAATCGT AGAGAAGATG 
CTGGACTTTA TGAGCATCAA TAAGGAGGAT CACACCACCG CAGACCTTGT GGGGAAAGTG 
GCAGAACTGG CAGAAAAATA TGCACCAGAC AACGAGTGGT TTGTTGAGAC CATGAACACG 
GTGTTCTCTG TAGGCGGAGA CACGTTGCAG CCCGACATCC CAAACAGTTT CCTTAAACTG 
CTCTCAGAAG GATTTGATGA TGAGGAGGAG AACAAGAAGT TGAGGCTGTT TGCGGTGAAC 
TCCTACATTT CTCTGCTTCA GGGAGAGCCC AGCAAGCTGC CCCAGCGCTT CCTCCAGGCC 
ATCTGCTGGG TTCTTGGAGA ATATTCTCAT TTACGGGAGG ATGTTGAGCC CGATCTGGTC 
TTGAGGCTGT TGGTTAAACT GTTGAATATA AAGAGCACCA GCAGTGAGAC TAAAAGCTGG 
ATCCTCATGG CGATGACCAA ACTCTGCTCG GGTGGAGCAC CTCTTTCCGT GCCTCTGGAG 
GTTTCTGAAA CATACAGCAG TTCCATGGAC ACAATGCTGC GACAAAGAGC TCAGGAGCTG 
CAGTACCTCA GCCAAAACCC TGAGCTACAG GCCAGGGTGC TACCTCGGGG TGTCAGCCAG 
GAGCCCATGG AGGTAGATTC ATCGCTGTCG TTTCTGGATG GATTTGTGTC AGAAGCGCTA 
GCTGCAGGCG CCGCTCCCTA TAAGCCTCCC CATCAAAGAC AGGAGGAACT GTCCCGATCC 
AAAGCACTGA GTTTGGAGCC CTATGGCCTG TCCCTGCCTA TCAGCATGTC CTCTTGCAGC 
ATCGCAGACA GACAGTCCCC TACGCTGCTG TCCGTGAGCT CTGGTCTGTC AGGCGACCGC 
ACGGATCACT CACACAAAGG CGGCTCCACA ACCTTGAAGC TGGATGGAGT GAGGAGGGTC 
TGGGGGAAAG AAGGTTATCT GGCTCAGAGA GAATCTGCAG AGGAAACTGC TCCGGTCGAG 
GCTCCCAGTC CGCTCCAGCC TCTCGTTCAG CAGGGTGACG GCGACGCCTC CTACAGCCAG 
ACTCCTACAC CAATCCCCGA ATGTGAACAG GAAAAACAGC AGCTGGCATC CTCACTGTTC 
GTTGGCCTGG CCTCCCAGAG CTCCGTGTGT CTGATGGGAA AATCTGAGCA AACATCTCAA 
CGATTCAGAA GAAAACTCAA AGATCCAGCT GCATCATTCC CCTCCAGCAC GCAGAACCCA 
CTGGTTTCTC CTCCCAGTAG TGTGGGTCAC CTGCTGCTGG ACTCCAGCAT TTCCTCAGAT 
CTAATTTCTT CACCTGAACA GACTCATCTA AGCTCTGTTG ACCTCACTGT TGCTAATGGA 
GAAGTAGAGT TACCTGACAG AGACATGAAG GGAATAAAAA GTACTCTACT TAGAGAAGAC 
TCCGCATTAG CAGCCGGTTC TTCTCCACAC GAAGAGCTAG ACAGACCTAA AGACTCGTGT 
CTCACTTCAC ATCTTCCAGC AGAACTTTCT GGACTCCCTC ACTCAGAAAT CACTCCCCTA 
TGCACCAGCC CAAGCCTGGA TCTGTCTTCC TGTCATGTTC AAAAAGACGA TTCTTTAGTG 
CTTCTCGTCT TCATGAAGAG CTCGTCTGAT TATGACATAC AGCAGATTCA AGTTCAAGTC 
ACCTGTGAGG AACTTGAGGT GTCCTGTGTG TGTGACAGTC CAGTAGAGGA GTTAAAAAGC 
CACGGCGTAG TGGTGTGTCA GTATTTTCTA ACGGTGAAGA GTCCCTCGGT TTACTTTGTG 
CTGTCTGGGA TGATGTCGCA CCAGATTCCT GATAATTCGC TTCAGGAAAC ACAATTCTCA 
CACAGAATTC CTTTAACCAG CTTCCTCAGA CCTTTGAGAC TTTCCACTGA GGAGTACGGG 
ACAATGTGGC TGGCCTTTTC ACACGACACC AAGCAGAATC TGCCGCTGAT GATGGACGCG 
GAGGAGCCGC TCACCGCTAC GCTGAAGGTG CTGAAGGACA AGCTTCAGCT TCATGTTGTA 
GAGATTATAG GAAACGAAGG GATTGTAGCT TGCTGCCTCA GACAGGATCA GCCGTGCCTC 
CTGCACTGTC GCGTTCACGC TGGCATGCTG GCGGTGTGGC TGCGCTCCCC CATCCCCGAG 
TTGCCCGACT GCCTCCTCTA CCACTGTCAG AGAGCGCTAC AGGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015819728.1
CDS	219..3389
Translation	MRQMKELMVRTIYCEMLGYEATFSYIHAIKLAQQGNVLEKRVGYLAVSLFLNESHELLLLLVNTVLKDLQSTNLIEVCMALTVVSQMFPKDMIPAILPLVEEKLNHSKEIIRRKAVLALYKFYLIAPNQVQHIHNRFRKALCDKDPGVMSASLHIYLQLIQENPESYKDLTASFVTILKQVVAGKLPMDFNYHSVPAPWLQIQLLRILSLLGKSDQSTSEVMYEILDESLRRAEMNHNITYAILYECVKCIYTIHPKSELLEKAAKVIGNFVLSQRINLKYLGLKALTYVVHQDPRLALQHQMTIIECLDHPDVIIKRETLELLFRITNDQNVTVIVEKMLDFMSINKEDHTTADLVGKVAELAEKYAPDNEWFVETMNTVFSVGGDTLQPDIPNSFLKLLSEGFDDEEENKKLRLFAVNSYISLLQGEPSKLPQRFLQAICWVLGEYSHLREDVEPDLVLRLLVKLLNIKSTSSETKSWILMAMTKLCSGGAPLSVPLEVSETYSSSMDTMLRQRAQELQYLSQNPELQARVLPRGVSQEPMEVDSSLSFLDGFVSEALAAGAAPYKPPHQRQEELSRSKALSLEPYGLSLPISMSSCSIADRQSPTLLSVSSGLSGDRTDHSHKGGSTTLKLDGVRRVWGKEGYLAQRESAEETAPVEAPSPLQPLVQQGDGDASYSQTPTPIPECEQEKQQLASSLFVGLASQSSVCLMGKSEQTSQRFRRKLKDPAASFPSSTQNPLVSPPSSVGHLLLDSSISSDLISSPEQTHLSSVDLTVANGEVELPDRDMKGIKSTLLREDSALAAGSSPHEELDRPKDSCLTSHLPAELSGLPHSEITPLCTSPSLDLSSCHVQKDDSLVLLVFMKSSSDYDIQQIQVQVTCEELEVSCVCDSPVEELKSHGVVVCQYFLTVKSPSVYFVLSGMMSHQIPDNSLQETQFSHRIPLTSFLRPLRLSTEEYGTMWLAFSHDTKQNLPLMMDAEEPLTATLKVLKDKLQLHVVEIIGNEGIVACCLRQDQPCLLHCRVHAGMLAVWLRSPIPELPDCLLYHCQRALQES

Target ORF information:

RefSeq Version	XM_015964242.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri adaptor related protein complex 4 epsilon 1 subunit (ap4e1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015964242.1

ORF Insert Sequence:

ATGAGGCAGA TGAAGGAGCT CATGGTGAGA ACCATCTACT GTGAGATGTT GGGCTACGAG 
GCAACGTTCA GTTATATTCA TGCCATTAAA CTGGCCCAGC AAGGGAATGT CCTGGAGAAG 
AGAGTTGGTT ATCTTGCTGT TTCCTTGTTT TTGAATGAAA GCCACGAGTT GCTGCTTCTC 
CTGGTAAACA CAGTGCTAAA GGATCTCCAG AGTACAAACC TGATTGAAGT GTGCATGGCT 
CTCACTGTTG TCAGCCAGAT GTTCCCTAAA GACATGATTC CTGCCATTCT TCCTCTGGTG 
GAGGAAAAGC TCAACCACTC CAAGGAAATC ATTCGACGAA AAGCCGTCCT GGCGTTGTAT 
AAATTCTACC TGATTGCCCC AAATCAAGTT CAGCACATTC ACAACAGGTT TCGTAAAGCG 
CTGTGTGACA AAGACCCCGG TGTGATGTCG GCTTCTTTAC ACATCTACCT ACAGCTGATT 
CAGGAGAATC CAGAAAGTTA CAAGGACCTC ACCGCAAGTT TTGTCACAAT CCTCAAGCAA 
GTGGTGGCCG GAAAACTTCC CATGGATTTC AACTATCACA GTGTTCCTGC TCCATGGCTT 
CAAATCCAGC TTCTCAGAAT TCTCTCCCTA CTTGGGAAAA GTGACCAGAG CACAAGTGAG 
GTCATGTATG AAATCCTGGA TGAGTCTTTA CGGAGAGCAG AGATGAATCA CAACATCACC 
TATGCTATTT TATACGAGTG TGTAAAATGC ATTTACACAA TTCATCCTAA ATCTGAGCTT 
TTGGAAAAAG CTGCGAAGGT CATCGGCAAT TTTGTTCTGT CACAGAGGAT AAATCTAAAG 
TATCTGGGCC TGAAGGCTCT CACCTATGTG GTCCATCAGG ATCCCAGACT GGCCTTGCAG 
CATCAGATGA CCATTATCGA GTGTCTCGAT CACCCCGATG TCATCATAAA GCGAGAGACG 
CTGGAGCTAC TCTTTCGGAT AACTAACGAC CAGAATGTAA CAGTAATCGT AGAGAAGATG 
CTGGACTTTA TGAGCATCAA TAAGGAGGAT CACACCACCG CAGACCTTGT GGGGAAAGTG 
GCAGAACTGG CAGAAAAATA TGCACCAGAC AACGAGTGGT TTGTTGAGAC CATGAACACG 
GTGTTCTCTG TAGGCGGAGA CACGTTGCAG CCCGACATCC CAAACAGTTT CCTTAAACTG 
CTCTCAGAAG GATTTGATGA TGAGGAGGAG AACAAGAAGT TGAGGCTGTT TGCGGTGAAC 
TCCTACATTT CTCTGCTTCA GGGAGAGCCC AGCAAGCTGC CCCAGCGCTT CCTCCAGGCC 
ATCTGCTGGG TTCTTGGAGA ATATTCTCAT TTACGGGAGG ATGTTGAGCC CGATCTGGTC 
TTGAGGCTGT TGGTTAAACT GTTGAATATA AAGAGCACCA GCAGTGAGAC TAAAAGCTGG 
ATCCTCATGG CGATGACCAA ACTCTGCTCG GGTGGAGCAC CTCTTTCCGT GCCTCTGGAG 
GTTTCTGAAA CATACAGCAG TTCCATGGAC ACAATGCTGC GACAAAGAGC TCAGGAGCTG 
CAGTACCTCA GCCAAAACCC TGAGCTACAG GCCAGGGTGC TACCTCGGGG TGTCAGCCAG 
GAGCCCATGG AGGTAGATTC ATCGCTGTCG TTTCTGGATG GATTTGTGTC AGAAGCGCTA 
GCTGCAGGCG CCGCTCCCTA TAAGCCTCCC CATCAAAGAC AGGAGGAACT GTCCCGATCC 
AAAGCACTGA GTTTGGAGCC CTATGGCCTG TCCCTGCCTA TCAGCATGTC CTCTTGCAGC 
ATCGCAGACA GACAGTCCCC TACGCTGCTG TCCGTGAGCT CTGGTCTGTC AGGCGACCGC 
ACGGATCACT CACACAAAGG CGGCTCCACA ACCTTGAAGC TGGATGGAGT GAGGAGGGTC 
TGGGGGAAAG AAGGTTATCT GGCTCAGAGA GAATCTGCAG AGGAAACTGC TCCGGTCGAG 
GCTCCCAGTC CGCTCCAGCC TCTCGTTCAG CAGGGTGACG GCGACGCCTC CTACAGCCAG 
ACTCCTACAC CAATCCCCGA ATGTGAACAG GAAAAACAGC AGCTGGCATC CTCACTGTTC 
GTTGGCCTGG CCTCCCAGAG CTCCGTGTGT CTGATGGGAA AATCTGAGCA AACATCTCAA 
CGATTCAGAA GAAAACTCAA AGATCCAGCT GCATCATTCC CCTCCAGCAC GCAGAACCCA 
CTGGTTTCTC CTCCCAGTAG TGTGGGTCAC CTGCTGCTGG ACTCCAGCAT TTCCTCAGAT 
CTAATTTCTT CACCTGAACA GACTCATCTA AGCTCTGTTG ACCTCACTGT TGCTAATGGA 
GAAGTAGAGT TACCTGACAG AGACATGAAG GGAATAAAAA GTACTCTACT TAGAGAAGAC 
TCCGCATTAG CAGCCGGTTC TTCTCCACAC GAAGAGCTAG ACAGACCTAA AGACTCGTGT 
CTCACTTCAC ATCTTCCAGC AGAACTTTCT GGACTCCCTC ACTCAGAAAT CACTCCCCTA 
TGCACCAGCC CAAGCCTGGA TCTGTCTTCC TGTCATGTTC AAAAAGACGA TTCTTTAGTG 
CTTCTCGTCT TCATGAAGAG CTCGTCTGAT TATGACATAC AGCAGATTCA AGTTCAAGTC 
ACCTGTGAGG AACTTGAGGT GTCCTGTGTG TGTGACAGTC CAGTAGAGGA GTTAAAAAGC 
CACGGCGTAG TGGTGTGTCA GTATTTTCTA ACGGTGAAGA GTCCCTCGGT TTACTTTGTG 
CTGTCTGGGA TGATGTCGCA CCAGATTCCT GATAATTCGC TTCAGGAAAC ACAATTCTCA 
CACAGAATTC CTTTAACCAG CTTCCTCAGA CCTTTGAGAC TTTCCACTGA GGAGTACGGG 
ACAATGTGGC TGGCCTTTTC ACACGACACC AAGCAGAATC TGCCGCTGAT GATGGACGCG 
GAGGAGCCGC TCACCGCTAC GCTGAAGGTG CTGAAGGACA AGCTTCAGCT TCATGTTGTA 
GAGATTATAG GAAACGAAGG GATTGTAGCT TGCTGCCTCA GACAGGATCA GCCGTGCCTC 
CTGCACTGTC GCGTTCACGC TGGCATGCTG GCGGTGTGGC TGCGCTCCCC CATCCCCGAG 
TTGCCCGACT GCCTCCTCTA CCACTGTCAG AGAGCGCTAC AGGAGAGCTG A