DGKI cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKI
Entrez Gene ID	104413432
Full Name	diacylglycerol kinase iota
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010024426.1	XP_010022728.1	diacylglycerol kinase iota

Pathways from BioSystems
Gene Ontology

The following DGKI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe111548	XM_010024426.1 Latest version!	Nestor notabilis diacylglycerol kinase, iota (DGKI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKI ( XM_010024426.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010022728.1 Nestor notabilis diacylglycerol kinase, iota (DGKI), mRNA.

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CloneID	ONe111548
Clone ID Related Accession (Same CDS sequence)	XM_010024426.1
Accession Version	XM_010024426.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	diacylglycerol kinase iota
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009916064.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGAGGCTCC 
AGATCTCCGA GAGAAAACTT TGTCCGCCAT CACTGGGTGC ACAGGAGGAG GCAGGAGGGG 
AAATGTAAAC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGTTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCCCTG GGGGCTCACG CTGCTGTTAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG GTGGTCTCCA TACCAGGAGA CGAGCCTGAT 
TGGGCAGGAG ACTGGAGCCC CGAAGGGGAA ACGGGCGTCT CCTACCCCTA TTGGGAGGGT 
TGGCAGAATT CGTTAAAGAC TTCAACACGG CGAAAGAAGA GAACATCTTT TAAAAGAAAA 
GCCAGCAAAA GAGGCAATGA AGATAACAAA GGTCGGCCAT TTGTGATAAA GCCTATCTCC 
TCTCCGCTCA TGAAGCCGCT GCTGGTTTTT GTCAATCCAA AAAGTGGAGG GAATCAGGGC 
ACAAAGGTTC TCCAAATGTT TATGTGGTAT TTGAATCCAC GCCAGGTCTT TGATCTCTCT 
CAGGAAGGGC CAAGAGATGC GCTGGAGCTG TATAGAAAAG TGCCAAACCT GCGGATCCTG 
GCCTGCGGTG GGGATGGGAC GGTGGGCTGG ATCCTCTCCA TCCTTGACGA GCTGCAACTC 
AGCCCCCCAC CTCCTGTGGC TGTCCTTCCA CTTGGCACTG GGAACGACCT TGCCCGCACT 
CTCAACTGGG GTGGGGGTTA TACAGATGAG CCAGTGTCCA AGATCCTGTG CCATGTGGAA 
GATGGGACCA TTGTCCAGCT GGACCGCTGG AATCTTCAGG TTGAGCGCAA CCCTGATTTG 
CCACAGGATG AGCTGGAGGA TGGGGCACGT AAGCTTCCTC TCAGTGTCTT CAATAATTAC 
TTCAGCCTTG GATTTGATGC ACATGTTACA CTGGAGTTCC ATGAATCTAG AGAAGCAAAT 
CCAGAGAAAT TCAACAGCCG ATTTAGAAAT AAAATGTTTT ATGCAGGGGC AGCCTTTACA 
GACTTTCTGC AAAGAAGCTC AAGAGATTTA TCCAAGCATG TTAAAGTTGT GTGTGATGGT 
ACAGACCTGA CTCCAAAGAT CCAGGAGCTG AAGTTCCAGT GTATAGTGTT TTTAAACATA 
CCCAGGTATT GTGCTGGCAC AATGCCCTGG GGAAACCCTG GAGATCACAG AGACTTTGAG 
CCTCAGCGCC ATGATGATGG CTACATTGAA GTCATTGGGT TCACCATGGC CTCACTGGCT 
GCTCTCCAGG TGGGTGGTCA TGGAGAGAGG TTGCATCAGT GCCGTGAGGT GACACTTTTA 
ACATACAAGT CTATCCCCAT GCAAGTGGAT GGTGAACCAT GTCGATTGGC TCCCTCACTC 
ATTCGGATCT CCTTAAGGAA CCAAGCCAAC ATGGTGCAGA AGAGCAAGAG GAGAACATCT 
ATGCCTTTGC TGAATGATCC TCATTCCATC CCAGATCGCC TGCGGATCAG AGTGAACAGG 
ATCAATTTAC AAGAATACGA GGGGCTACAT TATGACAAGG AGAAACTCCG GGAAGCTTCC 
ATTCCTCTTG GAATTATAGT TGTACGAGGA GACTGTGACT TGGAGACCTG TCGTATGTAC 
ATTGACCGTC TACAAGAGGA CCTACAGTCA GTAACTTCTA CTACACAGAG AGTTCATTAC 
CAGGACCAGG AAAGCTCTTT CCCCAGAGTA CTTTCTGTTC AGAGGCTCTC TCCTAGATGG 
TGCTTCCTAG ATGCAACTTC TGCTGATCGT TTTTACCGCA TTGACAGATC TCAGGAACAT 
TTGCACTTTG TGACGGAAAT TTCGCAGGAC GAGATTTTTA TTCTGGACCC AGAGATGGTA 
ATGTCACAGC AGGTGGGGAC GCCACCAGGA ATGCCTGATC TGGTAGTGGA GCAGGCCTCT 
GGAATATCAG ATCGGTGGAA CCCTGCCGTC CGAAAGAGGA TGCTGAGTGA CAGCGGCCTT 
GGCAAGATTT CCCCACACTA CGAGGTACCT GACAAACAAA AGGACGCCAG CCAGACACAC 
ATGCTGCAGT CACCGGTCTC TTCAGAAGAT CAAGCACTTT TACAGGCAGT CATAGCTGGT 
GATCTGCTGA AGTTCATAGA ATGCTGTAAA AAAGGAGCCA ATTTGTTAAT CCAAGGACCT 
GATCGCTGCT CCTTGTTGCA CCATGCTGCC AAAACAGGGC ATGGCGAGAT TGTGAAATAC 
ATCCTGGAAC ATGGTCCACC TGAATTACTG GACATGACAG ACAGCGAAAC GGGTGAGACA 
GCATTACATA AGGCAGCCTG CCAGCGCCAT CGTGCCGTCT GCCAGCTCCT GGTGGATGCA 
GGAGCCTCGC TGAGAAAGAC AGACTCCAAG GGTAAGACCC CTCGTGACAG GGCTCAGCAA 
GCTGGTGACC CAGATCTGGC TTCCTACCTG GAGAGTCGAC AAAACTACCA AATGGTTTCC 
CATGAGGATC TGGAGACAGC AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010022728.1
CDS	1..2607
Translation	MEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKLAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQVVSIPGDEPDWAGDWSPEGETGVSYPYWEGWQNSLKTSTRRKKRTSFKRKASKRGNEDNKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPRDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPPPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKILCHVEDGTIVQLDRWNLQVERNPDLPQDELEDGARKLPLSVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFTDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHRDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVTLLTYKSIPMQVDGEPCRLAPSLIRISLRNQANMVQKSKRRTSMPLLNDPHSIPDRLRIRVNRINLQEYEGLHYDKEKLREASIPLGIIVVRGDCDLETCRMYIDRLQEDLQSVTSTTQRVHYQDQESSFPRVLSVQRLSPRWCFLDATSADRFYRIDRSQEHLHFVTEISQDEIFILDPEMVMSQQVGTPPGMPDLVVEQASGISDRWNPAVRKRMLSDSGLGKISPHYEVPDKQKDASQTHMLQSPVSSEDQALLQAVIAGDLLKFIECCKKGANLLIQGPDRCSLLHHAAKTGHGEIVKYILEHGPPELLDMTDSETGETALHKAACQRHRAVCQLLVDAGASLRKTDSKGKTPRDRAQQAGDPDLASYLESRQNYQMVSHEDLETAV

Target ORF information:

RefSeq Version	XM_010024426.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis diacylglycerol kinase, iota (DGKI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010024426.1

ORF Insert Sequence:

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGAGGCTCC 
AGATCTCCGA GAGAAAACTT TGTCCGCCAT CACTGGGTGC ACAGGAGGAG GCAGGAGGGG 
AAATGTAAAC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGTTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCCCTG GGGGCTCACG CTGCTGTTAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG GTGGTCTCCA TACCAGGAGA CGAGCCTGAT 
TGGGCAGGAG ACTGGAGCCC CGAAGGGGAA ACGGGCGTCT CCTACCCCTA TTGGGAGGGT 
TGGCAGAATT CGTTAAAGAC TTCAACACGG CGAAAGAAGA GAACATCTTT TAAAAGAAAA 
GCCAGCAAAA GAGGCAATGA AGATAACAAA GGTCGGCCAT TTGTGATAAA GCCTATCTCC 
TCTCCGCTCA TGAAGCCGCT GCTGGTTTTT GTCAATCCAA AAAGTGGAGG GAATCAGGGC 
ACAAAGGTTC TCCAAATGTT TATGTGGTAT TTGAATCCAC GCCAGGTCTT TGATCTCTCT 
CAGGAAGGGC CAAGAGATGC GCTGGAGCTG TATAGAAAAG TGCCAAACCT GCGGATCCTG 
GCCTGCGGTG GGGATGGGAC GGTGGGCTGG ATCCTCTCCA TCCTTGACGA GCTGCAACTC 
AGCCCCCCAC CTCCTGTGGC TGTCCTTCCA CTTGGCACTG GGAACGACCT TGCCCGCACT 
CTCAACTGGG GTGGGGGTTA TACAGATGAG CCAGTGTCCA AGATCCTGTG CCATGTGGAA 
GATGGGACCA TTGTCCAGCT GGACCGCTGG AATCTTCAGG TTGAGCGCAA CCCTGATTTG 
CCACAGGATG AGCTGGAGGA TGGGGCACGT AAGCTTCCTC TCAGTGTCTT CAATAATTAC 
TTCAGCCTTG GATTTGATGC ACATGTTACA CTGGAGTTCC ATGAATCTAG AGAAGCAAAT 
CCAGAGAAAT TCAACAGCCG ATTTAGAAAT AAAATGTTTT ATGCAGGGGC AGCCTTTACA 
GACTTTCTGC AAAGAAGCTC AAGAGATTTA TCCAAGCATG TTAAAGTTGT GTGTGATGGT 
ACAGACCTGA CTCCAAAGAT CCAGGAGCTG AAGTTCCAGT GTATAGTGTT TTTAAACATA 
CCCAGGTATT GTGCTGGCAC AATGCCCTGG GGAAACCCTG GAGATCACAG AGACTTTGAG 
CCTCAGCGCC ATGATGATGG CTACATTGAA GTCATTGGGT TCACCATGGC CTCACTGGCT 
GCTCTCCAGG TGGGTGGTCA TGGAGAGAGG TTGCATCAGT GCCGTGAGGT GACACTTTTA 
ACATACAAGT CTATCCCCAT GCAAGTGGAT GGTGAACCAT GTCGATTGGC TCCCTCACTC 
ATTCGGATCT CCTTAAGGAA CCAAGCCAAC ATGGTGCAGA AGAGCAAGAG GAGAACATCT 
ATGCCTTTGC TGAATGATCC TCATTCCATC CCAGATCGCC TGCGGATCAG AGTGAACAGG 
ATCAATTTAC AAGAATACGA GGGGCTACAT TATGACAAGG AGAAACTCCG GGAAGCTTCC 
ATTCCTCTTG GAATTATAGT TGTACGAGGA GACTGTGACT TGGAGACCTG TCGTATGTAC 
ATTGACCGTC TACAAGAGGA CCTACAGTCA GTAACTTCTA CTACACAGAG AGTTCATTAC 
CAGGACCAGG AAAGCTCTTT CCCCAGAGTA CTTTCTGTTC AGAGGCTCTC TCCTAGATGG 
TGCTTCCTAG ATGCAACTTC TGCTGATCGT TTTTACCGCA TTGACAGATC TCAGGAACAT 
TTGCACTTTG TGACGGAAAT TTCGCAGGAC GAGATTTTTA TTCTGGACCC AGAGATGGTA 
ATGTCACAGC AGGTGGGGAC GCCACCAGGA ATGCCTGATC TGGTAGTGGA GCAGGCCTCT 
GGAATATCAG ATCGGTGGAA CCCTGCCGTC CGAAAGAGGA TGCTGAGTGA CAGCGGCCTT 
GGCAAGATTT CCCCACACTA CGAGGTACCT GACAAACAAA AGGACGCCAG CCAGACACAC 
ATGCTGCAGT CACCGGTCTC TTCAGAAGAT CAAGCACTTT TACAGGCAGT CATAGCTGGT 
GATCTGCTGA AGTTCATAGA ATGCTGTAAA AAAGGAGCCA ATTTGTTAAT CCAAGGACCT 
GATCGCTGCT CCTTGTTGCA CCATGCTGCC AAAACAGGGC ATGGCGAGAT TGTGAAATAC 
ATCCTGGAAC ATGGTCCACC TGAATTACTG GACATGACAG ACAGCGAAAC GGGTGAGACA 
GCATTACATA AGGCAGCCTG CCAGCGCCAT CGTGCCGTCT GCCAGCTCCT GGTGGATGCA 
GGAGCCTCGC TGAGAAAGAC AGACTCCAAG GGTAAGACCC CTCGTGACAG GGCTCAGCAA 
GCTGGTGACC CAGATCTGGC TTCCTACCTG GAGAGTCGAC AAAACTACCA AATGGTTTCC 
CATGAGGATC TGGAGACAGC AGTCTGA