ALKBH8 cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALKBH8
Entrez Gene ID	104409818
Full Name	alkB homolog 8, tRNA methyltransferase
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010019807.1	XP_010018109.1	alkylated DNA repair protein alkB homolog 8

Pathways from BioSystems
Gene Ontology

The following ALKBH8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALKBH8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe116797	XM_010019807.1 Latest version!	Nestor notabilis alkB, alkylation repair homolog 8 (E. coli) (ALKBH8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALKBH8 ( XM_010019807.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010018109.1 Nestor notabilis alkB, alkylation repair homolog 8 (E. coli) (ALKBH8), mRNA.

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CloneID	ONe116797
Clone ID Related Accession (Same CDS sequence)	XM_010019807.1
Accession Version	XM_010019807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	alkylated DNA repair protein alkB homolog 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009939383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTTA AAACCAGCAA AGATCATTTG AAAGTCCAGA AAATGAAGAA GAAGGTATTG 
AGGAAGCAAA TCAGAGCTCA GCACACATTG ATGAGACATG AGGGCATTGA GTGCATCTCC 
GATGCCACAC AGAGCCTGGT TATTGCCAAT GCTGGTCTTG GTAATGGGAT GAGCAGACAC 
CAACTGCTTA GGATAGTAGA AGAATATGGA TTGGTGGAAA CAGTCTTAAT GCCACCGAGT 
AAGCCATATT CATTTGTGAA ATATGGGACA ATAGAAGAAG CAAAGAAAGC CTTTGATGCC 
CTTAATGGAA AAGAAGTAAT ATTGGAAGAC TGTGGCCAAA ATATTGTTCT ATATATTAAT 
TTTGTGGAAA AAGTTTTCTG GAAGAATGCT GTTCCTACAA GCTTGCCTCC AGGCCTAATG 
GTCATTGAAG AGATTATTTC TCCAGAGGAA GAAAGGAGAC TATTGGAAAG TGTTGACTGG 
ATAGGAGGTG AAGACACTCA GAAAGCCCAG AAGACTTTAA AGCATAGAAG AGTAAAACAT 
TTTGGCTATG AGTTTTGCTA TGATAACAAC AATATCGATA AAGACAAACC TTTACCTGGA 
GGTCTTCCTG AAATTTGTGA CCCATTCTTG GAGAAATGCT TGAAGCAGGG ATATATCAAA 
CATAAACCTG ATCAACTGAC TGTAAATCAG TATGAACCTG GACAAGGAAT TCCTCCTCAT 
ATTGATACAC ATTCCGCATT TGAGGATGAA ATAATCTCTC TCAGTTTAGG AGCTGAGATT 
GTGATGGATT TCAAACACCC TGATGGCCAT ACAGTGGCAA TTATGCTGCC CCGATGCAGT 
TTATTGGTGA TGGCTGGAGA ATCCAGATAC CTGTGGACAC ATGGGATTGC ACCCCGAAAA 
TATGATGTCA TTCAAGCATC TGAGCTTGGG CAGAAACTTG GAACTATCAC TGCTGATGCT 
GGAGATTTAA CACTGAATCG AAGGGAAACA AGGACATCAT TTACATTCAG GAAAGTAAGG 
AGAAGCCCTT GCAATTGCAT TTACCCATCT GTCTGTGACA GCCAGAAAGG ACAGCAAAGC 
TGGGTACAGC CTTCGTTTCC TCACAGCGAG ATGGAGGCCT CTAAGCTGGA GCAAGAATAT 
GTACACAAGG TGTATGAAGA GATTGCTACA CACTTCAGCA GTACCAGGCA TAGCCCATGG 
CCCCGAATTG TGGAGTTTCT CAGGTCCCTA CCAAAAGGAT CAATAGTGGC TGATGTTGGT 
TGTGGTAATG GGAAGTATCT GGGCATCAAC GAGGACTTAT ACATGGTTGG CTGTGACCGC 
AGCAAGAACC TGGTGGATAT TTGTGGAGAG AAAAATCTCG AGGCTTTTGT CTGCGATGCG 
CTGTCAGTGC CAGTGCGCAG CGGTTCTTGT GACGCCTGCA TCTCGATTGC TGTAATCCAC 
CATTTCTCAA CAGTGGAGAG GAGACTGGCT ACTATCCGTG AACTCACCCG GCTTCTGAGA 
CCTGGTGGAA CAGCACTGAT TTATGTCTGG GCAATGGAAC AAGAATACAA AAACCAGAAG 
TCTAAATACC TTAAGGAAAA AAATGGCAGT AAAGAGGAGG AAATTAATAA TAGCACAGCC 
CAGAGACCCC TCAGTGATCA AATGTTGAGC AGCAGTCGAG ACTCAGCATG TTCTGACCAG 
TTCCTTAGTG ACCTGAAGGA CAAAGGCTGT GATGCAAAGC CCACGGCAGC TACCACACTG 
CCCATTCACA CTAACAGAAC TTCCTTTCAC TCTCAGGATT TGCTGGTTCC CTGGCACCTG 
AAAGGTGGCT CTAAAAAGAA AGGGGAAAGC ATAGATACAA GGTTATTTCC AGTTGGCTCC 
AAGGAATCAC AAGAACTCAG TCCTGTTTTC CACCGCTATT ACCATGTGTT CTGTGAAGGG 
GAACTGGAAG CAGTGTGCAG ATCCCTGGAT TGTGTGAGAG TTCAAAGGAG TTACTATGAT 
CAGGGAAACT GGTGTGTGGT TCTAGAAAAG TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010018109.1
CDS	1..2016
Translation	MELKTSKDHLKVQKMKKKVLRKQIRAQHTLMRHEGIECISDATQSLVIANAGLGNGMSRHQLLRIVEEYGLVETVLMPPSKPYSFVKYGTIEEAKKAFDALNGKEVILEDCGQNIVLYINFVEKVFWKNAVPTSLPPGLMVIEEIISPEEERRLLESVDWIGGEDTQKAQKTLKHRRVKHFGYEFCYDNNNIDKDKPLPGGLPEICDPFLEKCLKQGYIKHKPDQLTVNQYEPGQGIPPHIDTHSAFEDEIISLSLGAEIVMDFKHPDGHTVAIMLPRCSLLVMAGESRYLWTHGIAPRKYDVIQASELGQKLGTITADAGDLTLNRRETRTSFTFRKVRRSPCNCIYPSVCDSQKGQQSWVQPSFPHSEMEASKLEQEYVHKVYEEIATHFSSTRHSPWPRIVEFLRSLPKGSIVADVGCGNGKYLGINEDLYMVGCDRSKNLVDICGEKNLEAFVCDALSVPVRSGSCDACISIAVIHHFSTVERRLATIRELTRLLRPGGTALIYVWAMEQEYKNQKSKYLKEKNGSKEEEINNSTAQRPLSDQMLSSSRDSACSDQFLSDLKDKGCDAKPTAATTLPIHTNRTSFHSQDLLVPWHLKGGSKKKGESIDTRLFPVGSKESQELSPVFHRYYHVFCEGELEAVCRSLDCVRVQRSYYDQGNWCVVLEKL

Target ORF information:

RefSeq Version	XM_010019807.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis alkB, alkylation repair homolog 8 (E. coli) (ALKBH8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010019807.1

ORF Insert Sequence:

ATGGAACTTA AAACCAGCAA AGATCATTTG AAAGTCCAGA AAATGAAGAA GAAGGTATTG 
AGGAAGCAAA TCAGAGCTCA GCACACATTG ATGAGACATG AGGGCATTGA GTGCATCTCC 
GATGCCACAC AGAGCCTGGT TATTGCCAAT GCTGGTCTTG GTAATGGGAT GAGCAGACAC 
CAACTGCTTA GGATAGTAGA AGAATATGGA TTGGTGGAAA CAGTCTTAAT GCCACCGAGT 
AAGCCATATT CATTTGTGAA ATATGGGACA ATAGAAGAAG CAAAGAAAGC CTTTGATGCC 
CTTAATGGAA AAGAAGTAAT ATTGGAAGAC TGTGGCCAAA ATATTGTTCT ATATATTAAT 
TTTGTGGAAA AAGTTTTCTG GAAGAATGCT GTTCCTACAA GCTTGCCTCC AGGCCTAATG 
GTCATTGAAG AGATTATTTC TCCAGAGGAA GAAAGGAGAC TATTGGAAAG TGTTGACTGG 
ATAGGAGGTG AAGACACTCA GAAAGCCCAG AAGACTTTAA AGCATAGAAG AGTAAAACAT 
TTTGGCTATG AGTTTTGCTA TGATAACAAC AATATCGATA AAGACAAACC TTTACCTGGA 
GGTCTTCCTG AAATTTGTGA CCCATTCTTG GAGAAATGCT TGAAGCAGGG ATATATCAAA 
CATAAACCTG ATCAACTGAC TGTAAATCAG TATGAACCTG GACAAGGAAT TCCTCCTCAT 
ATTGATACAC ATTCCGCATT TGAGGATGAA ATAATCTCTC TCAGTTTAGG AGCTGAGATT 
GTGATGGATT TCAAACACCC TGATGGCCAT ACAGTGGCAA TTATGCTGCC CCGATGCAGT 
TTATTGGTGA TGGCTGGAGA ATCCAGATAC CTGTGGACAC ATGGGATTGC ACCCCGAAAA 
TATGATGTCA TTCAAGCATC TGAGCTTGGG CAGAAACTTG GAACTATCAC TGCTGATGCT 
GGAGATTTAA CACTGAATCG AAGGGAAACA AGGACATCAT TTACATTCAG GAAAGTAAGG 
AGAAGCCCTT GCAATTGCAT TTACCCATCT GTCTGTGACA GCCAGAAAGG ACAGCAAAGC 
TGGGTACAGC CTTCGTTTCC TCACAGCGAG ATGGAGGCCT CTAAGCTGGA GCAAGAATAT 
GTACACAAGG TGTATGAAGA GATTGCTACA CACTTCAGCA GTACCAGGCA TAGCCCATGG 
CCCCGAATTG TGGAGTTTCT CAGGTCCCTA CCAAAAGGAT CAATAGTGGC TGATGTTGGT 
TGTGGTAATG GGAAGTATCT GGGCATCAAC GAGGACTTAT ACATGGTTGG CTGTGACCGC 
AGCAAGAACC TGGTGGATAT TTGTGGAGAG AAAAATCTCG AGGCTTTTGT CTGCGATGCG 
CTGTCAGTGC CAGTGCGCAG CGGTTCTTGT GACGCCTGCA TCTCGATTGC TGTAATCCAC 
CATTTCTCAA CAGTGGAGAG GAGACTGGCT ACTATCCGTG AACTCACCCG GCTTCTGAGA 
CCTGGTGGAA CAGCACTGAT TTATGTCTGG GCAATGGAAC AAGAATACAA AAACCAGAAG 
TCTAAATACC TTAAGGAAAA AAATGGCAGT AAAGAGGAGG AAATTAATAA TAGCACAGCC 
CAGAGACCCC TCAGTGATCA AATGTTGAGC AGCAGTCGAG ACTCAGCATG TTCTGACCAG 
TTCCTTAGTG ACCTGAAGGA CAAAGGCTGT GATGCAAAGC CCACGGCAGC TACCACACTG 
CCCATTCACA CTAACAGAAC TTCCTTTCAC TCTCAGGATT TGCTGGTTCC CTGGCACCTG 
AAAGGTGGCT CTAAAAAGAA AGGGGAAAGC ATAGATACAA GGTTATTTCC AGTTGGCTCC 
AAGGAATCAC AAGAACTCAG TCCTGTTTTC CACCGCTATT ACCATGTGTT CTGTGAAGGG 
GAACTGGAAG CAGTGTGCAG ATCCCTGGAT TGTGTGAGAG TTCAAAGGAG TTACTATGAT 
CAGGGAAACT GGTGTGTGGT TCTAGAAAAG TTGTAG