ALPI cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALPI
Entrez Gene ID	104407200
Full Name	alkaline phosphatase, intestinal
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010016413.1	XP_010014715.1	intestinal-type alkaline phosphatase

Pathways from BioSystems
Gene Ontology

The following ALPI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALPI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe115187	XM_010016413.1 Latest version!	Nestor notabilis alkaline phosphatase, intestinal (ALPI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALPI ( XM_010016413.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010014715.1 Nestor notabilis alkaline phosphatase, intestinal (ALPI), mRNA.

Price & Availability↑

CloneID	ONe115187
Clone ID Related Accession (Same CDS sequence)	XM_010016413.1
Accession Version	XM_010016413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	intestinal-type alkaline phosphatase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009932471.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCC CAGCACTTCT CATCCTCTGC CTGGGCCTCC AGGCAGAGCT CAGCACTGCT 
GTCATCCCAG TGGAGGAGGA GGATCCAGCC TTCTGGAACA GGCAGGCAGC TTCAGCCATT 
GAGGCCTCCT TCAAGCTGCA GCCCAGAGTA ACCGAAGCCA AAAACCTCAT CATCTTCCTC 
GGGGATGGAT TCGGGGTCCC CTCCATCACG GCCACCCGCA TTCTAAACGG GCAGCAGCAG 
GGGAAGTTGG GCCCTGAGAC CCCCCTCGCC CTGGATTCTT TCCCCTATGT GGCTCTCTCC 
AAGACATACA GTGTGGACCG GATTGTCCCT GACAGCGCAT CCACATCCAC AGCCTACCTC 
TGCGGCGTGA AGGGCAACTA CCACACAGCA GGAGTGAGTG CAGCCGCTCG CCTCTCTCAG 
TGCAACACCA CAGCAGGCAA TGAGGTGGTC TCAGTGCTGG AGCGAGCACG CAAAGCCGGG 
AAGGCAGTGG GCATAGTGAC AACATCACGG GTACAGCACG CATCACCGTC GGGGGCCTAT 
GCCCATGTGG TGGACCGCAA CTGGTACGCG GATGTCAGCA TACCCGAGGA GGCCCGTCTC 
CAGGGCTGCA AGGACATCGC CTGGCAGCTG GTCCACAATG TCGACATCAA TGTGATCATG 
GGGGGTGGTC GGATATACAT GACCCCCGCA GGGACGCCAG ACCCTGAATA TCCCACCTAT 
GAAACAGAGA ATGGGATACG GGAAGATGGA AAAAACCTCA TCAACATGTG GCGGGAGGCA 
CGGCCGGGTG CCCGCTATGT CTGGAACAGG ACAGAGATGC TGGCTGCTGC TGCTGACCCC 
AACGTGACTT ATTTGATGGG TCTCTTTGAA CCCATGGATA TGAAGTATGA CCTGGTGCGT 
AACACCACCC TGGACCCGTC ACTCACTGAG ATGACAGAGG CAGCCATCAC CATCCTGAGC 
AGAAACCCCA AAGGCTTCTT CCTGTTTGTG GAAGGTGGCA GGATCGACCA TGGCCACCAC 
GAAGGTGCAG CCCAGAAGGC ACTGACAGAT GGGATTGCAT TTGATCAGGC CATTGACCGG 
GCGGGCATCC TGACAGACGA GGAGGACACC CTCACCGTGG TCACTGCTGA CCATTCACAC 
GTCTTCAGCT TTGGTGGCTA CACCCTGCGT GGCTCCTCCA TCTTAGGTCT GGCTCCAGAC 
AAAGCTGCTG ACAATAAGAG CTACACGTCC ATCCTCTATG GGAATGGGCC AGGCTACCTG 
GGTGCTGTTC GGCCCAACGT GAATGAATCC ACAGCCAATG AATTCGACTA CGTGCAGCAG 
GCAGCTGTGC CCCTCAAGTC AGAGTCCCAT GGTGGTGAGG ATGTGGCCAT TCTGGCCAAG 
GGCCCCATGG CCCACCTCTT CCATGGGGTG CAGGAGCAGA CCTACGTGGC CCATGCCATG 
GCTTACGCTG CCTGCCTCGA GCCCTATGTC GACTGCCACC AGCGGAACTC TGCCACTGCA 
GCCTGCACCA CCCTCCTGGC CCTGCTCATC CCCACCCTCT TCCTGTTCCT CTTCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010014715.1
CDS	1..1560
Translation	MKLPALLILCLGLQAELSTAVIPVEEEDPAFWNRQAASAIEASFKLQPRVTEAKNLIIFLGDGFGVPSITATRILNGQQQGKLGPETPLALDSFPYVALSKTYSVDRIVPDSASTSTAYLCGVKGNYHTAGVSAAARLSQCNTTAGNEVVSVLERARKAGKAVGIVTTSRVQHASPSGAYAHVVDRNWYADVSIPEEARLQGCKDIAWQLVHNVDINVIMGGGRIYMTPAGTPDPEYPTYETENGIREDGKNLINMWREARPGARYVWNRTEMLAAAADPNVTYLMGLFEPMDMKYDLVRNTTLDPSLTEMTEAAITILSRNPKGFFLFVEGGRIDHGHHEGAAQKALTDGIAFDQAIDRAGILTDEEDTLTVVTADHSHVFSFGGYTLRGSSILGLAPDKAADNKSYTSILYGNGPGYLGAVRPNVNESTANEFDYVQQAAVPLKSESHGGEDVAILAKGPMAHLFHGVQEQTYVAHAMAYAACLEPYVDCHQRNSATAACTTLLALLIPTLFLFLFH

Target ORF information:

RefSeq Version	XM_010016413.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis alkaline phosphatase, intestinal (ALPI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010016413.1

ORF Insert Sequence:

ATGAAGCTCC CAGCACTTCT CATCCTCTGC CTGGGCCTCC AGGCAGAGCT CAGCACTGCT 
GTCATCCCAG TGGAGGAGGA GGATCCAGCC TTCTGGAACA GGCAGGCAGC TTCAGCCATT 
GAGGCCTCCT TCAAGCTGCA GCCCAGAGTA ACCGAAGCCA AAAACCTCAT CATCTTCCTC 
GGGGATGGAT TCGGGGTCCC CTCCATCACG GCCACCCGCA TTCTAAACGG GCAGCAGCAG 
GGGAAGTTGG GCCCTGAGAC CCCCCTCGCC CTGGATTCTT TCCCCTATGT GGCTCTCTCC 
AAGACATACA GTGTGGACCG GATTGTCCCT GACAGCGCAT CCACATCCAC AGCCTACCTC 
TGCGGCGTGA AGGGCAACTA CCACACAGCA GGAGTGAGTG CAGCCGCTCG CCTCTCTCAG 
TGCAACACCA CAGCAGGCAA TGAGGTGGTC TCAGTGCTGG AGCGAGCACG CAAAGCCGGG 
AAGGCAGTGG GCATAGTGAC AACATCACGG GTACAGCACG CATCACCGTC GGGGGCCTAT 
GCCCATGTGG TGGACCGCAA CTGGTACGCG GATGTCAGCA TACCCGAGGA GGCCCGTCTC 
CAGGGCTGCA AGGACATCGC CTGGCAGCTG GTCCACAATG TCGACATCAA TGTGATCATG 
GGGGGTGGTC GGATATACAT GACCCCCGCA GGGACGCCAG ACCCTGAATA TCCCACCTAT 
GAAACAGAGA ATGGGATACG GGAAGATGGA AAAAACCTCA TCAACATGTG GCGGGAGGCA 
CGGCCGGGTG CCCGCTATGT CTGGAACAGG ACAGAGATGC TGGCTGCTGC TGCTGACCCC 
AACGTGACTT ATTTGATGGG TCTCTTTGAA CCCATGGATA TGAAGTATGA CCTGGTGCGT 
AACACCACCC TGGACCCGTC ACTCACTGAG ATGACAGAGG CAGCCATCAC CATCCTGAGC 
AGAAACCCCA AAGGCTTCTT CCTGTTTGTG GAAGGTGGCA GGATCGACCA TGGCCACCAC 
GAAGGTGCAG CCCAGAAGGC ACTGACAGAT GGGATTGCAT TTGATCAGGC CATTGACCGG 
GCGGGCATCC TGACAGACGA GGAGGACACC CTCACCGTGG TCACTGCTGA CCATTCACAC 
GTCTTCAGCT TTGGTGGCTA CACCCTGCGT GGCTCCTCCA TCTTAGGTCT GGCTCCAGAC 
AAAGCTGCTG ACAATAAGAG CTACACGTCC ATCCTCTATG GGAATGGGCC AGGCTACCTG 
GGTGCTGTTC GGCCCAACGT GAATGAATCC ACAGCCAATG AATTCGACTA CGTGCAGCAG 
GCAGCTGTGC CCCTCAAGTC AGAGTCCCAT GGTGGTGAGG ATGTGGCCAT TCTGGCCAAG 
GGCCCCATGG CCCACCTCTT CCATGGGGTG CAGGAGCAGA CCTACGTGGC CCATGCCATG 
GCTTACGCTG CCTGCCTCGA GCCCTATGTC GACTGCCACC AGCGGAACTC TGCCACTGCA 
GCCTGCACCA CCCTCCTGGC CCTGCTCATC CCCACCCTCT TCCTGTTCCT CTTCCACTGA