EDEM1 cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	EDEM1
Entrez Gene ID	104406408
Full Name	ER degradation enhancing alpha-mannosidase like protein 1
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010015376.1	XP_010013678.1	ER degradation-enhancing alpha-mannosidase-like protein 1

Pathways from BioSystems
Gene Ontology

The following EDEM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDEM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe114672	XM_010015376.1 Latest version!	Nestor notabilis ER degradation enhancer, mannosidase alpha-like 1 (EDEM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EDEM1 ( XM_010015376.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010013678.1 Nestor notabilis ER degradation enhancer, mannosidase alpha-like 1 (EDEM1), mRNA.

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CloneID	ONe114672
Clone ID Related Accession (Same CDS sequence)	XM_010015376.1
Accession Version	XM_010015376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	ER degradation-enhancing alpha-mannosidase-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009930404.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CTTCAGAATT CCAGAAAGCA GTGAAGTTGG TGATTGACAC AGTTTCATTT 
GATAAAGACT CAACAGTCCA AGTTTTTGAG GCAACAATCA GGGTTTTGGG AAGCTTGCTT 
TCTGCCCACA TAATAATCAC AGATACCAAA CAGCCTTTTG GTGATATGAC CATTAAGGAT 
TATGACAATG AACTGTTGCA TATGGCTCAT GACCTAGCAG TGAGACTACT TCCAGCCTTT 
GAGAACACCA AAACTGGAAT TCCATATCCT CGGGTCAATC TGAAGAAGGG GGTACCTCCA 
GACAGCTATA ATGAAACTTG TACTGCAGGA GCTGGTTCCT TGCTGGTGGA ATTTGGGATC 
CTTAGCAGGC TGCTTGGTGA TTCCACATTT GAATGGGTGG CCAGGAGAGC CGTCAAAGCA 
CTCTGGAGTC TCAGGAGCAA TAACACTGGG CTGCTAGGAA ATGTTGTGAA TATTCAAACT 
GGTCATTGGG TTGGAAAGCA AAGTGGATTG GGAGCAGGGT CGGATTCATT CTATGAATAC 
CTTCTGAAGT CTTACATCCT CTTTGGAGAA AAGGAAGACT TGGAGATGTT CAATGATGCT 
TATCGGAATA TTCAGAACCA CTTAAGAAGA GGTCGAGAAG CTTGCAATGA AGGTGAAGGT 
GACCCTCCTT TGTATGTTAA CGTAAACATG TTCACAGGAC AGCTGATGAA CACGTGGATT 
GACTCTTTGC AAGCCTTTTT TCCTGGACTA CAGGTATTGA TAGGAGATGT GGAAGATGCT 
ATTTGTCTCC ATGCTTTTTA TTATGCCATA TGGAAACGAT ACGGAGCTCT CCCAGAGAGA 
TACAACTGGC AATTGCAAGC GCCGGATGTT CCCTTTTATC CACTGAGACC AGAGTTAGTG 
GAATCCACGT ATCTTCTTTA TCAGGCCACA AAGAACCCGT TTTACCTTCA CGTGGGGATG 
GATATTCTGC AGAGTTTGGA AAAATACACA AAAGCAAAGT GTGGCTATGC CACATTACAC 
CACGTTGTAG AGAAGACAAA GGAAGACCGA ATGGAGAGCT TTTTTCTCAG TGAAACATGT 
AAATACTTGT ACCTGTTGTT TGATGAAGAA AATCCAGTGC ATAAATCTGG AAATAAGTAT 
ATGTTTACTA CTGAGGGGCA TATTGTGTCT GTGGATGAAC GTTTTCGTAA CTCACTGTGG 
CAAGACATCC TCCCTGGAGA AGAGAACGGC GCAGAAAAAA TTAAACCTAA TGAGTTAAAG 
GCAGTCAACT TCAGCTCTAA TTGTAACAGA GTTCCTGATG AGAGGAGGTA TTTGCTGCCC 
TTGAAGAGTA ATTACATGAG ACAGATTGAT CGAATGGTGG GCTTGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010013678.1
CDS	3..1373
Translation	MGNSSEFQKAVKLVIDTVSFDKDSTVQVFEATIRVLGSLLSAHIIITDTKQPFGDMTIKDYDNELLHMAHDLAVRLLPAFENTKTGIPYPRVNLKKGVPPDSYNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWSLRSNNTGLLGNVVNIQTGHWVGKQSGLGAGSDSFYEYLLKSYILFGEKEDLEMFNDAYRNIQNHLRRGREACNEGEGDPPLYVNVNMFTGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVPFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKAKCGYATLHHVVEKTKEDRMESFFLSETCKYLYLLFDEENPVHKSGNKYMFTTEGHIVSVDERFRNSLWQDILPGEENGAEKIKPNELKAVNFSSNCNRVPDERRYLLPLKSNYMRQIDRMVGLI

Target ORF information:

RefSeq Version	XM_010015376.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis ER degradation enhancer, mannosidase alpha-like 1 (EDEM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010015376.1

ORF Insert Sequence:

ATGGGAAACT CTTCAGAATT CCAGAAAGCA GTGAAGTTGG TGATTGACAC AGTTTCATTT 
GATAAAGACT CAACAGTCCA AGTTTTTGAG GCAACAATCA GGGTTTTGGG AAGCTTGCTT 
TCTGCCCACA TAATAATCAC AGATACCAAA CAGCCTTTTG GTGATATGAC CATTAAGGAT 
TATGACAATG AACTGTTGCA TATGGCTCAT GACCTAGCAG TGAGACTACT TCCAGCCTTT 
GAGAACACCA AAACTGGAAT TCCATATCCT CGGGTCAATC TGAAGAAGGG GGTACCTCCA 
GACAGCTATA ATGAAACTTG TACTGCAGGA GCTGGTTCCT TGCTGGTGGA ATTTGGGATC 
CTTAGCAGGC TGCTTGGTGA TTCCACATTT GAATGGGTGG CCAGGAGAGC CGTCAAAGCA 
CTCTGGAGTC TCAGGAGCAA TAACACTGGG CTGCTAGGAA ATGTTGTGAA TATTCAAACT 
GGTCATTGGG TTGGAAAGCA AAGTGGATTG GGAGCAGGGT CGGATTCATT CTATGAATAC 
CTTCTGAAGT CTTACATCCT CTTTGGAGAA AAGGAAGACT TGGAGATGTT CAATGATGCT 
TATCGGAATA TTCAGAACCA CTTAAGAAGA GGTCGAGAAG CTTGCAATGA AGGTGAAGGT 
GACCCTCCTT TGTATGTTAA CGTAAACATG TTCACAGGAC AGCTGATGAA CACGTGGATT 
GACTCTTTGC AAGCCTTTTT TCCTGGACTA CAGGTATTGA TAGGAGATGT GGAAGATGCT 
ATTTGTCTCC ATGCTTTTTA TTATGCCATA TGGAAACGAT ACGGAGCTCT CCCAGAGAGA 
TACAACTGGC AATTGCAAGC GCCGGATGTT CCCTTTTATC CACTGAGACC AGAGTTAGTG 
GAATCCACGT ATCTTCTTTA TCAGGCCACA AAGAACCCGT TTTACCTTCA CGTGGGGATG 
GATATTCTGC AGAGTTTGGA AAAATACACA AAAGCAAAGT GTGGCTATGC CACATTACAC 
CACGTTGTAG AGAAGACAAA GGAAGACCGA ATGGAGAGCT TTTTTCTCAG TGAAACATGT 
AAATACTTGT ACCTGTTGTT TGATGAAGAA AATCCAGTGC ATAAATCTGG AAATAAGTAT 
ATGTTTACTA CTGAGGGGCA TATTGTGTCT GTGGATGAAC GTTTTCGTAA CTCACTGTGG 
CAAGACATCC TCCCTGGAGA AGAGAACGGC GCAGAAAAAA TTAAACCTAA TGAGTTAAAG 
GCAGTCAACT TCAGCTCTAA TTGTAACAGA GTTCCTGATG AGAGGAGGTA TTTGCTGCCC 
TTGAAGAGTA ATTACATGAG ACAGATTGAT CGAATGGTGG GCTTGATCTG A