EVI5 cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	EVI5
Entrez Gene ID	104405285
Full Name	ecotropic viral integration site 5
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010013950.1	XP_010012252.1	ecotropic viral integration site 5 protein homolog isoform X2
XM_010013949.1	XP_010012251.1	ecotropic viral integration site 5 protein homolog isoform X1

Pathways from BioSystems
Gene Ontology

The following EVI5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EVI5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe114013	XM_010013949.1 Latest version!	Nestor notabilis ecotropic viral integration site 5 (EVI5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ONe114014	XM_010013950.1 Latest version!	Nestor notabilis ecotropic viral integration site 5 (EVI5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EVI5 ( XM_010013949.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010012251.1 Nestor notabilis ecotropic viral integration site 5 (EVI5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONe114013
Clone ID Related Accession (Same CDS sequence)	XM_010013949.1
Accession Version	XM_010013949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	ecotropic viral integration site 5 protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009927174.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGTC AGGTGGCAAG TCCATCTGCC TCATTACACA CTACATCCTC CTCTACCACT 
CTGTCTACAC CCGTGCTATC GCCATCCTCA CCAACACAGC TGAGTCCAGA CGACTTAGAG 
TTACTGGCTA AACTTGAGGA ACAGAACAGA CTCTTGGAAA CAGATAGCAA ATCCTTGCGA 
TCAGTAAATG GGTCAAGAAG AAATAGTGGA TCTTCTCTTG TATCTAGTTC ATCAGCTTCT 
AGCAATCTCA GCCATCTTGA GGAAGACTCA TGGATCCTCT GGGGGAGGAT TGTGAATGAA 
TGGGAAGATG TGCGGAAAAA GAAAGAAAAG CAAGTTAAGG AGCTTGTTCG AAAAGGGATA 
CCTCATCATT TCAGAGCAAT TGTTTGGCAA CTCTTATGCA GTGCTCAAAG CATGCCGATT 
AAGGATCAGT ATTCAGAGCT CTTGAAAATG ACATCTCCTT GTGAGAAACT AATAAGAAGG 
GACATTGCTA GAACATACCC TGAACATGAT TTTTTTAAGG AAAAAGACAG CCTTGGGCAG 
GAGGTCTTGT TCAATGTCAT GAAGGCTTAT TCTTTGGTGG ATCGTGAGGT TGGATACTGT 
CAAGGAAGCG CTTTTATAGT TGGACTACTG CTTATGCAGA TGCCAGAAGA AGAGGCATTC 
TGTGTGTTTG TTAAATTAAT GCAAGATTAC AGACTACGAG AACTCTTTAA ACCAAGCATG 
GCTGAACTGG GCCTTTGTAT GTACCAGTTT GAATGCATGA TACAGGAGCA TCTTCCAGAG 
CTTTACGTGC ACTTTCAGTC TCAGAGTTTC CACACTTCTA TGTATGCATC GTCATGGTTT 
TTAACTATAT TCCTTACAAC TTTTCCACTA CCAATTGCAA CAAGAGTATT TGATATCTTT 
ATGTCTGAGG GTTTAGAGAT AGTATTTCGA GTAGGTTTAG CAGTACTTCA GATGAACCAA 
GCAGAGTTAC TGCAACTTGA CATGGAAGGA ATGTTACAGC ACTTTCAAAA GGTCATTCCA 
CATCAGTTTG ACACTGGTCC AGACAAATTA ATTCAAGCAT CTTACCAAGT CAAATACAAT 
GCAAAAAAGA TGAAAAAGTT AGAAAAGGAA TACACTACAA TAAAGACAAA AGAAATGGAA 
GAACAAGTTG AAATTAAAAG GCTACGAACT GAAAATAGAC TCTTGAAACA GCGCATTGAA 
ACACTAGAAA AAGAAAGTGC TTCCTTGGCA GATAGATTGA TACAGGGACA AGTGACAAGG 
GCCCAGGAGG CTGAGGAAAA CTACCTTGTA AAACGGGAGC TGGCCACCAT CAAACAGCAG 
AGTGATGAGG CCATTACTAA ACTGGAGCAA GCTGAGAATA CCATCAGGGA GCTCCAACAG 
CAGCAGCAAT GGCATAAATG CAGTTCACAA TACAGTGAAG ACTTTGTGCT ACAGCTGGAA 
AAAGAGTTGG TCCAAGCCAG GCTGAGTGAA GCAGAATCCC ATTGTGCCCT GAAGGAGATG 
CAAGATAAAG TTCTAGAGAT GGAAAAGAGG AACAGCTCGC TCCCTGATGA GGAAAATGTT 
GCTAGACTAC AAGAAGAACT GATTGCAGTA AAATTAAGAG AAGCAGAAGC TCTGATGGGT 
CTCAAAGAAC TAAGGCAACA AGTCAAAGAT TTGGAGGAAC ACTGGCAGCG TCATCTAGCT 
CGTACCACTG GAAGATGGAA AGATCCCCCC AGAAAAAACA CAGTGAATGA GCTACAGGAT 
GAGCTGATGA CAGTCCGATT GAGAGAAGCG GAAACTCAGG CTGAACTGAA AGAGACCAAA 
CAAAGAATGA TGGAGATGGA AACACAGAAT CAGATCAATA GTAACCACTT ACGAAGGGCA 
GAGCAGGAGG TTACCAACTT ACAGGGGAAG GTACAGTATC TTTCTGCACA GAACAAAGGA 
CTCCTTGCTC AGTTGAATGA AGCAAAACGT AGACAAGCAG AGATTGAATG CAAGAGTAAA 
GAAGAGGTGA TGGCAGTCCG GCTCCGTGAG GCAGACCGCA TTGCAGCTGT GGCTGAGCTC 
CAACAGCATA TTGCTGAACT AGAAATCCAG AAAGAAGAAG GAAAAATTCA AGGGCAGCTT 
AATAAATCTG ATTCTAACCA GTACATCAGA GAGCTGAAAG ATCAGATAGC TGAGCTGCAC 
CATGAGATCA AATGTCTAAA AGGCCAGAGA GACTTCTCAG ACCAACCCAC TTTTGATGGG 
ATTCACATTG TCAACCATTT CACAGGAGAT GATGAATCAT TTCATTCTTC TGATGAAGAT 
TTTATAACTA ACTCCATACA GGAGAGTGGG GTGAACTTTC ATCTACATAG GAGGACTGGT 
CAGATGGCTT TGGATCACAC AGTTGTAGAC AGCAGTGACA GTGATGCTGA GGAAAGTGCC 
CTTCAGACTG AGAATTCAGA CAAGATGGTT TCCAAGCAGA GAAGAATGGA GTCTTACTCT 
ACTACTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010012251.1
CDS	80..2551
Translation	MASQVASPSASLHTTSSSTTLSTPVLSPSSPTQLSPDDLELLAKLEEQNRLLETDSKSLRSVNGSRRNSGSSLVSSSSASSNLSHLEEDSWILWGRIVNEWEDVRKKKEKQVKELVRKGIPHHFRAIVWQLLCSAQSMPIKDQYSELLKMTSPCEKLIRRDIARTYPEHDFFKEKDSLGQEVLFNVMKAYSLVDREVGYCQGSAFIVGLLLMQMPEEEAFCVFVKLMQDYRLRELFKPSMAELGLCMYQFECMIQEHLPELYVHFQSQSFHTSMYASSWFLTIFLTTFPLPIATRVFDIFMSEGLEIVFRVGLAVLQMNQAELLQLDMEGMLQHFQKVIPHQFDTGPDKLIQASYQVKYNAKKMKKLEKEYTTIKTKEMEEQVEIKRLRTENRLLKQRIETLEKESASLADRLIQGQVTRAQEAEENYLVKRELATIKQQSDEAITKLEQAENTIRELQQQQQWHKCSSQYSEDFVLQLEKELVQARLSEAESHCALKEMQDKVLEMEKRNSSLPDEENVARLQEELIAVKLREAEALMGLKELRQQVKDLEEHWQRHLARTTGRWKDPPRKNTVNELQDELMTVRLREAETQAELKETKQRMMEMETQNQINSNHLRRAEQEVTNLQGKVQYLSAQNKGLLAQLNEAKRRQAEIECKSKEEVMAVRLREADRIAAVAELQQHIAELEIQKEEGKIQGQLNKSDSNQYIRELKDQIAELHHEIKCLKGQRDFSDQPTFDGIHIVNHFTGDDESFHSSDEDFITNSIQESGVNFHLHRRTGQMALDHTVVDSSDSDAEESALQTENSDKMVSKQRRMESYSTTV

Target ORF information:

RefSeq Version	XM_010013949.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis ecotropic viral integration site 5 (EVI5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010013949.1

ORF Insert Sequence:

ATGGCCAGTC AGGTGGCAAG TCCATCTGCC TCATTACACA CTACATCCTC CTCTACCACT 
CTGTCTACAC CCGTGCTATC GCCATCCTCA CCAACACAGC TGAGTCCAGA CGACTTAGAG 
TTACTGGCTA AACTTGAGGA ACAGAACAGA CTCTTGGAAA CAGATAGCAA ATCCTTGCGA 
TCAGTAAATG GGTCAAGAAG AAATAGTGGA TCTTCTCTTG TATCTAGTTC ATCAGCTTCT 
AGCAATCTCA GCCATCTTGA GGAAGACTCA TGGATCCTCT GGGGGAGGAT TGTGAATGAA 
TGGGAAGATG TGCGGAAAAA GAAAGAAAAG CAAGTTAAGG AGCTTGTTCG AAAAGGGATA 
CCTCATCATT TCAGAGCAAT TGTTTGGCAA CTCTTATGCA GTGCTCAAAG CATGCCGATT 
AAGGATCAGT ATTCAGAGCT CTTGAAAATG ACATCTCCTT GTGAGAAACT AATAAGAAGG 
GACATTGCTA GAACATACCC TGAACATGAT TTTTTTAAGG AAAAAGACAG CCTTGGGCAG 
GAGGTCTTGT TCAATGTCAT GAAGGCTTAT TCTTTGGTGG ATCGTGAGGT TGGATACTGT 
CAAGGAAGCG CTTTTATAGT TGGACTACTG CTTATGCAGA TGCCAGAAGA AGAGGCATTC 
TGTGTGTTTG TTAAATTAAT GCAAGATTAC AGACTACGAG AACTCTTTAA ACCAAGCATG 
GCTGAACTGG GCCTTTGTAT GTACCAGTTT GAATGCATGA TACAGGAGCA TCTTCCAGAG 
CTTTACGTGC ACTTTCAGTC TCAGAGTTTC CACACTTCTA TGTATGCATC GTCATGGTTT 
TTAACTATAT TCCTTACAAC TTTTCCACTA CCAATTGCAA CAAGAGTATT TGATATCTTT 
ATGTCTGAGG GTTTAGAGAT AGTATTTCGA GTAGGTTTAG CAGTACTTCA GATGAACCAA 
GCAGAGTTAC TGCAACTTGA CATGGAAGGA ATGTTACAGC ACTTTCAAAA GGTCATTCCA 
CATCAGTTTG ACACTGGTCC AGACAAATTA ATTCAAGCAT CTTACCAAGT CAAATACAAT 
GCAAAAAAGA TGAAAAAGTT AGAAAAGGAA TACACTACAA TAAAGACAAA AGAAATGGAA 
GAACAAGTTG AAATTAAAAG GCTACGAACT GAAAATAGAC TCTTGAAACA GCGCATTGAA 
ACACTAGAAA AAGAAAGTGC TTCCTTGGCA GATAGATTGA TACAGGGACA AGTGACAAGG 
GCCCAGGAGG CTGAGGAAAA CTACCTTGTA AAACGGGAGC TGGCCACCAT CAAACAGCAG 
AGTGATGAGG CCATTACTAA ACTGGAGCAA GCTGAGAATA CCATCAGGGA GCTCCAACAG 
CAGCAGCAAT GGCATAAATG CAGTTCACAA TACAGTGAAG ACTTTGTGCT ACAGCTGGAA 
AAAGAGTTGG TCCAAGCCAG GCTGAGTGAA GCAGAATCCC ATTGTGCCCT GAAGGAGATG 
CAAGATAAAG TTCTAGAGAT GGAAAAGAGG AACAGCTCGC TCCCTGATGA GGAAAATGTT 
GCTAGACTAC AAGAAGAACT GATTGCAGTA AAATTAAGAG AAGCAGAAGC TCTGATGGGT 
CTCAAAGAAC TAAGGCAACA AGTCAAAGAT TTGGAGGAAC ACTGGCAGCG TCATCTAGCT 
CGTACCACTG GAAGATGGAA AGATCCCCCC AGAAAAAACA CAGTGAATGA GCTACAGGAT 
GAGCTGATGA CAGTCCGATT GAGAGAAGCG GAAACTCAGG CTGAACTGAA AGAGACCAAA 
CAAAGAATGA TGGAGATGGA AACACAGAAT CAGATCAATA GTAACCACTT ACGAAGGGCA 
GAGCAGGAGG TTACCAACTT ACAGGGGAAG GTACAGTATC TTTCTGCACA GAACAAAGGA 
CTCCTTGCTC AGTTGAATGA AGCAAAACGT AGACAAGCAG AGATTGAATG CAAGAGTAAA 
GAAGAGGTGA TGGCAGTCCG GCTCCGTGAG GCAGACCGCA TTGCAGCTGT GGCTGAGCTC 
CAACAGCATA TTGCTGAACT AGAAATCCAG AAAGAAGAAG GAAAAATTCA AGGGCAGCTT 
AATAAATCTG ATTCTAACCA GTACATCAGA GAGCTGAAAG ATCAGATAGC TGAGCTGCAC 
CATGAGATCA AATGTCTAAA AGGCCAGAGA GACTTCTCAG ACCAACCCAC TTTTGATGGG 
ATTCACATTG TCAACCATTT CACAGGAGAT GATGAATCAT TTCATTCTTC TGATGAAGAT 
TTTATAACTA ACTCCATACA GGAGAGTGGG GTGAACTTTC ATCTACATAG GAGGACTGGT 
CAGATGGCTT TGGATCACAC AGTTGTAGAC AGCAGTGACA GTGATGCTGA GGAAAGTGCC 
CTTCAGACTG AGAATTCAGA CAAGATGGTT TCCAAGCAGA GAAGAATGGA GTCTTACTCT 
ACTACTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVI5 ( XM_010013950.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010012252.1 Nestor notabilis ecotropic viral integration site 5 (EVI5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONe114014
Clone ID Related Accession (Same CDS sequence)	XM_010013950.1
Accession Version	XM_010013950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	ecotropic viral integration site 5 protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009927174.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCTG TGGCGACAGA CAAGGTTGCA GGAAAGCTGA GCTCTACTCT CTCATGGGTG 
AAGAACACCG TCTCGCACAC CGTCAGTCAA ATGGCCAGTC AGGTGGCAAG TCCATCTGCC 
TCATTACACA CTACATCCTC CTCTACCACT CTGTCTACAC CCGTGCTATC GCCATCCTCA 
CCAACACAGC TGAGTCCAGA CGACTTAGAG TTACTGGCTA AACTTGAGGA ACAGAACAGA 
CTCTTGGAAA CAGATAGCAA ATCCTTGCGA TCAGTAAATG GGTCAAGAAG AAATAGTGGA 
TCTTCTCTTG TATCTAGTTC ATCAGCTTCT AGCAATCTCA GCCATCTTGA GGAAGACTCA 
TGGATCCTCT GGGGGAGGAT TGTGAATGAA TGGGAAGATG TGCGGAAAAA GAAAGAAAAG 
CAAGTTAAGG AGCTTGTTCG AAAAGGGATA CCTCATCATT TCAGAGCAAT TGTTTGGCAA 
CTCTTATGCA GTGCTCAAAG CATGCCGATT AAGGATCAGT ATTCAGAGCT CTTGAAAATG 
ACATCTCCTT GTGAGAAACT AATAAGAAGG GACATTGCTA GAACATACCC TGAACATGAT 
TTTTTTAAGG AAAAAGACAG CCTTGGGCAG GAGGTCTTGT TCAATGTCAT GAAGGCTTAT 
TCTTTGGTGG ATCGTGAGGT TGGATACTGT CAAGGAAGCG CTTTTATAGT TGGACTACTG 
CTTATGCAGA TGCCAGAAGA AGAGGCATTC TGTGTGTTTG TTAAATTAAT GCAAGATTAC 
AGACTACGAG AACTCTTTAA ACCAAGCATG GCTGAACTGG GCCTTTGTAT GTACCAGTTT 
GAATGCATGA TACAGGAGCA TCTTCCAGAG CTTTACGTGC ACTTTCAGTC TCAGAGTTTC 
CACACTTCTA TGTATGCATC GTCATGGTTT TTAACTATAT TCCTTACAAC TTTTCCACTA 
CCAATTGCAA CAAGAGTATT TGATATCTTT ATGTCTGAGG GTTTAGAGAT AGTATTTCGA 
GTAGGTTTAG CAGTACTTCA GATGAACCAA GCAGAGTTAC TGCAACTTGA CATGGAAGGA 
ATGTTACAGC ACTTTCAAAA GGTCATTCCA CATCAGTTTG ACACTGGTCC AGACAAATTA 
ATTCAAGCAT CTTACCAAGT CAAATACAAT GCAAAAAAGA TGAAAAAGTT AGAAAAGGAA 
TACACTACAA TAAAGACAAA AGAAATGGAA GAACAAGTTG AAATTAAAAG GCTACGAACT 
GAAAATAGAC TCTTGAAACA GCGCATTGAA ACACTAGAAA AACATAAATG CAGTTCACAA 
TACAGTGAAG ACTTTGTGCT ACAGCTGGAA AAAGAGTTGG TCCAAGCCAG GCTGAGTGAA 
GCAGAATCCC ATTGTGCCCT GAAGGAGATG CAAGATAAAG TTCTAGAGAT GGAAAAGAGG 
AACAGCTCGC TCCCTGATGA GGAAAATGTT GCTAGACTAC AAGAAGAACT GATTGCAGTA 
AAATTAAGAG AAGCAGAAGC TCTGATGGGT CTCAAAGAAC TAAGGCAACA AGTCAAAGAT 
TTGGAGGAAC ACTGGCAGCG TCATCTAGCT CGTACCACTG GAAGATGGAA AGATCCCCCC 
AGAAAAAACA CAGTGAATGA GCTACAGGAT GAGCTGATGA CAGTCCGATT GAGAGAAGCG 
GAAACTCAGG CTGAACTGAA AGAGACCAAA CAAAGAATGA TGGAGATGGA AACACAGAAT 
CAGATCAATA GTAACCACTT ACGAAGGGCA GAGCAGGAGG TTACCAACTT ACAGGGGAAG 
GTACAGTATC TTTCTGCACA GAACAAAGGA CTCCTTGCTC AGTTGAATGA AGCAAAACGT 
AGACAAGCAG AGATTGAATG CAAGAGTAAA GAAGAGGTGA TGGCAGTCCG GCTCCGTGAG 
GCAGACCGCA TTGCAGCTGT GGCTGAGCTC CAACAGCATA TTGCTGAACT AGAAATCCAG 
AAAGAAGAAG GAAAAATTCA AGGGCAGCTT AATAAATCTG ATTCTAACCA GTACATCAGA 
GAGCTGAAAG ATCAGATAGC TGAGCTGCAC CATGAGATCA AATGTCTAAA AGGCCAGAGA 
GACTTCTCAG ACCAACCCAC TTTTGATGGG ATTCACATTG TCAACCATTT CACAGGAGAT 
GATGAATCAT TTCATTCTTC TGATGAAGAT TTTATAACTA ACTCCATACA GGAGAGTGGG 
GTGAACTTTC ATCTACATAG GAGGACTGGT CAGATGGCTT TGGATCACAC AGTTGTAGAC 
AGCAGTGACA GTGATGCTGA GGAAAGTGCC CTTCAGACTG AGAATTCAGA CAAGATGGTT 
TCCAAGCAGA GAAGAATGGA GTCTTACTCT ACTACTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010012252.1
CDS	1..2382
Translation	MSSVATDKVAGKLSSTLSWVKNTVSHTVSQMASQVASPSASLHTTSSSTTLSTPVLSPSSPTQLSPDDLELLAKLEEQNRLLETDSKSLRSVNGSRRNSGSSLVSSSSASSNLSHLEEDSWILWGRIVNEWEDVRKKKEKQVKELVRKGIPHHFRAIVWQLLCSAQSMPIKDQYSELLKMTSPCEKLIRRDIARTYPEHDFFKEKDSLGQEVLFNVMKAYSLVDREVGYCQGSAFIVGLLLMQMPEEEAFCVFVKLMQDYRLRELFKPSMAELGLCMYQFECMIQEHLPELYVHFQSQSFHTSMYASSWFLTIFLTTFPLPIATRVFDIFMSEGLEIVFRVGLAVLQMNQAELLQLDMEGMLQHFQKVIPHQFDTGPDKLIQASYQVKYNAKKMKKLEKEYTTIKTKEMEEQVEIKRLRTENRLLKQRIETLEKHKCSSQYSEDFVLQLEKELVQARLSEAESHCALKEMQDKVLEMEKRNSSLPDEENVARLQEELIAVKLREAEALMGLKELRQQVKDLEEHWQRHLARTTGRWKDPPRKNTVNELQDELMTVRLREAETQAELKETKQRMMEMETQNQINSNHLRRAEQEVTNLQGKVQYLSAQNKGLLAQLNEAKRRQAEIECKSKEEVMAVRLREADRIAAVAELQQHIAELEIQKEEGKIQGQLNKSDSNQYIRELKDQIAELHHEIKCLKGQRDFSDQPTFDGIHIVNHFTGDDESFHSSDEDFITNSIQESGVNFHLHRRTGQMALDHTVVDSSDSDAEESALQTENSDKMVSKQRRMESYSTTV

Target ORF information:

RefSeq Version	XM_010013950.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis ecotropic viral integration site 5 (EVI5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010013950.1

ORF Insert Sequence:

ATGAGCTCTG TGGCGACAGA CAAGGTTGCA GGAAAGCTGA GCTCTACTCT CTCATGGGTG 
AAGAACACCG TCTCGCACAC CGTCAGTCAA ATGGCCAGTC AGGTGGCAAG TCCATCTGCC 
TCATTACACA CTACATCCTC CTCTACCACT CTGTCTACAC CCGTGCTATC GCCATCCTCA 
CCAACACAGC TGAGTCCAGA CGACTTAGAG TTACTGGCTA AACTTGAGGA ACAGAACAGA 
CTCTTGGAAA CAGATAGCAA ATCCTTGCGA TCAGTAAATG GGTCAAGAAG AAATAGTGGA 
TCTTCTCTTG TATCTAGTTC ATCAGCTTCT AGCAATCTCA GCCATCTTGA GGAAGACTCA 
TGGATCCTCT GGGGGAGGAT TGTGAATGAA TGGGAAGATG TGCGGAAAAA GAAAGAAAAG 
CAAGTTAAGG AGCTTGTTCG AAAAGGGATA CCTCATCATT TCAGAGCAAT TGTTTGGCAA 
CTCTTATGCA GTGCTCAAAG CATGCCGATT AAGGATCAGT ATTCAGAGCT CTTGAAAATG 
ACATCTCCTT GTGAGAAACT AATAAGAAGG GACATTGCTA GAACATACCC TGAACATGAT 
TTTTTTAAGG AAAAAGACAG CCTTGGGCAG GAGGTCTTGT TCAATGTCAT GAAGGCTTAT 
TCTTTGGTGG ATCGTGAGGT TGGATACTGT CAAGGAAGCG CTTTTATAGT TGGACTACTG 
CTTATGCAGA TGCCAGAAGA AGAGGCATTC TGTGTGTTTG TTAAATTAAT GCAAGATTAC 
AGACTACGAG AACTCTTTAA ACCAAGCATG GCTGAACTGG GCCTTTGTAT GTACCAGTTT 
GAATGCATGA TACAGGAGCA TCTTCCAGAG CTTTACGTGC ACTTTCAGTC TCAGAGTTTC 
CACACTTCTA TGTATGCATC GTCATGGTTT TTAACTATAT TCCTTACAAC TTTTCCACTA 
CCAATTGCAA CAAGAGTATT TGATATCTTT ATGTCTGAGG GTTTAGAGAT AGTATTTCGA 
GTAGGTTTAG CAGTACTTCA GATGAACCAA GCAGAGTTAC TGCAACTTGA CATGGAAGGA 
ATGTTACAGC ACTTTCAAAA GGTCATTCCA CATCAGTTTG ACACTGGTCC AGACAAATTA 
ATTCAAGCAT CTTACCAAGT CAAATACAAT GCAAAAAAGA TGAAAAAGTT AGAAAAGGAA 
TACACTACAA TAAAGACAAA AGAAATGGAA GAACAAGTTG AAATTAAAAG GCTACGAACT 
GAAAATAGAC TCTTGAAACA GCGCATTGAA ACACTAGAAA AACATAAATG CAGTTCACAA 
TACAGTGAAG ACTTTGTGCT ACAGCTGGAA AAAGAGTTGG TCCAAGCCAG GCTGAGTGAA 
GCAGAATCCC ATTGTGCCCT GAAGGAGATG CAAGATAAAG TTCTAGAGAT GGAAAAGAGG 
AACAGCTCGC TCCCTGATGA GGAAAATGTT GCTAGACTAC AAGAAGAACT GATTGCAGTA 
AAATTAAGAG AAGCAGAAGC TCTGATGGGT CTCAAAGAAC TAAGGCAACA AGTCAAAGAT 
TTGGAGGAAC ACTGGCAGCG TCATCTAGCT CGTACCACTG GAAGATGGAA AGATCCCCCC 
AGAAAAAACA CAGTGAATGA GCTACAGGAT GAGCTGATGA CAGTCCGATT GAGAGAAGCG 
GAAACTCAGG CTGAACTGAA AGAGACCAAA CAAAGAATGA TGGAGATGGA AACACAGAAT 
CAGATCAATA GTAACCACTT ACGAAGGGCA GAGCAGGAGG TTACCAACTT ACAGGGGAAG 
GTACAGTATC TTTCTGCACA GAACAAAGGA CTCCTTGCTC AGTTGAATGA AGCAAAACGT 
AGACAAGCAG AGATTGAATG CAAGAGTAAA GAAGAGGTGA TGGCAGTCCG GCTCCGTGAG 
GCAGACCGCA TTGCAGCTGT GGCTGAGCTC CAACAGCATA TTGCTGAACT AGAAATCCAG 
AAAGAAGAAG GAAAAATTCA AGGGCAGCTT AATAAATCTG ATTCTAACCA GTACATCAGA 
GAGCTGAAAG ATCAGATAGC TGAGCTGCAC CATGAGATCA AATGTCTAAA AGGCCAGAGA 
GACTTCTCAG ACCAACCCAC TTTTGATGGG ATTCACATTG TCAACCATTT CACAGGAGAT 
GATGAATCAT TTCATTCTTC TGATGAAGAT TTTATAACTA ACTCCATACA GGAGAGTGGG 
GTGAACTTTC ATCTACATAG GAGGACTGGT CAGATGGCTT TGGATCACAC AGTTGTAGAC 
AGCAGTGACA GTGATGCTGA GGAAAGTGCC CTTCAGACTG AGAATTCAGA CAAGATGGTT 
TCCAAGCAGA GAAGAATGGA GTCTTACTCT ACTACTGTGT GA