DIS3L cDNA ORF clone, Neomonachus schauinslandi(Hawaiian monk seal)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIS3L
Entrez Gene ID	110583882
Full Name	DIS3 like exosome 3'-5' exoribonuclease
Gene Type	protein-coding
Organism	Neomonachus schauinslandi(Hawaiian monk seal)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021693908.1	XP_021549583.1	DIS3-like exonuclease 1 isoform X1
XM_021693909.1	XP_021549584.1	DIS3-like exonuclease 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following DIS3L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIS3L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe146399	XM_021693908.1 Latest version!	Neomonachus schauinslandi DIS3 like exosome 3'-5' exoribonuclease (DIS3L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ONe146400	XM_021693909.1 Latest version!	Neomonachus schauinslandi DIS3 like exosome 3'-5' exoribonuclease (DIS3L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIS3L ( XM_021693908.1 ) cDNA ORF clone, Neomonachus schauinslandi(Hawaiian monk seal) -> XP_021549583.1 Neomonachus schauinslandi DIS3 like exosome 3'-5' exoribonuclease (DIS3L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONe146399
Clone ID Related Accession (Same CDS sequence)	XM_021693908.1
Accession Version	XM_021693908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-27
Organism	Neomonachus schauinslandi(Hawaiian monk seal)
Product	DIS3-like exonuclease 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018734350.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Neomonachus schauinslandi Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGTT CTCTTCTGCC TGATGGGAAA CTCTTGTCTA GTGATGTGAC TCATTACATG 
ATCCCAGATT GGAAAGTTGT TCAAGATTAC CTGGAGATCC TTGAGTTCCC AGAGTTGAAG 
GGAATTATTT TCATGCAGAC GGCTTGTCAA ACTGTGCAGC ATCAAAAAGG CCGCAGACAG 
TATAACAAAT TGCGAAATCT CCTGAAGGAT GCACGTCATG ATTGCATTCT CTTTGCTAAT 
GAATTCCAGC AGCACTGCTA TCTCCCTCGA GAAAGAGGAG AATCCATGGA AAAGTGGCAG 
ACCAGGAGCA TATACAATGC AGCCGTGTGG TACTTTCACC ACTGCCAGGA TAGGATGCCA 
ATCGTTATGG TGACAGAAGA CAAAGAGGCA ATTCAGCAGT ATGGAAGTGA GACAGAAGGA 
GTGTTTGTGA TTTCTTTCAA GAATTACCTG GACAATTTTT GGCCCGACTT AAAGGCCGCC 
CACGAGCTCT GTGACTCTAT CCTTCAGTCT CGACGGGAAA GAGAGAATGA GAGTCAGGAG 
AGCCACGGGA AGGAGTACAC AGAACATCTT CCCCTGGAAG TGTTAGAAGC CGGCATTAAG 
TCTGGGCGCT ACATCCAGGG AATCCTGAAT GTCAACAAAC ACAGGGCACA AATAGAAGCT 
TTCGTTCGAC TTCAAGGAGC CAGCTGTAAA GATTCAGGTT TAGTCAGTGA CATCCTCATC 
CACGGGATGA AGGCTCGAAA CCGCTCGATC CATGGAGACG TGGTAGTCGT GGAATTGCTC 
CCTAGAAACG AATGGAAAGG AAGAACGGCT GCCCTGTGTG AGAATGACAG TGAGGACAAG 
GCCTTGGGAG ACCCCCTGAG TGAGCCCATG CCCACAGGCA GAGTGGTGGG CATCCTTCAG 
AAGAACTGGC GGGATTATGT GGTGACATTT CCGTCCAAAG AAGAGGTCCA GTCTCAGGGC 
AAAAACGCTC AGAAAATCCT AGTTACACCT TGGGATTACA GAATTCCCAA AATCCGCATT 
AGCACTCAGC AAGCAGAAGC GCTCCAGGAC TTCCGGGTGG TTGTACGCAT CGATTCCTGG 
GAGTCGACAT CTGTGTATCC AAATGGACAT TTTGTGCGTG TTTTAGGAAG AATCGGGGAC 
CTGGAAGGGG AAATCGCAAC CATCTTGGTG GAAAACAATA TTTCGGTTGT CCCTTTCTCC 
GAAGCTCAGA TGTGTGAGAT GCCAGTCAAC ACACCAGGAA ATCCCTGGAA AGTGAGTCCT 
GAAGAGGAAC GAGAACGGAA AGACTTGAGG GAAACCCATC TTGTGTTCAG CATTGACCCA 
AAAGGCTGTG AAGATGTGGA CGACACGCTC TCAGTCAGAA CCTTAACTAA TGGCAACTTG 
GAACTTGGGG TCCACATTGC AGACGTAACG CACTTCGTGC CACCAAATTC TTATATCGAC 
ATTGAAGCTA GGACAAGGGC CACCACTTAC TATTTAGCAG ACCGTCGCTA TGACATGCTG 
CCCTCCATCC TCAGCGCTGA TTTGTGCTCT CTTCTGGGAG GCGTTGATAG GTACGCCGTA 
AGTGTCATAT GGGAATTGGA TAAAACCTCC TATGAAATTA AGAAAGTGTG GTACGGCAGA 
ACCATTATTC GATCAGCATA CAAACTGTTT TATGAAGCAG CCCAGGAACT GCTGGACGGA 
AACCTCCACA TTGTTGATGA TATTCCAGAG TTCAGAGATT TGGATGAGAA GAGCAGACAA 
GCCAAGCTAG AGGAGTTAGT GTGGGCCATC AGCAAGTTGA CGGACATAGC GCGCCACATC 
CGGGCTAAGC GAGACCATTG CGGTGCCCTC GAACTGGAGG GGGTAGAGAT TCGTGTTCAG 
CTGGATGAGA AAAAGAACAT CCACGACCTC ATCCCCAAGC AGCCCCTGGA AGTCCACGAG 
ACGGTGGCCG AATGCATGAT CCTGGCCAAC CACTGGGTAG CCAAGAAGAT CTGGGAGAGC 
TTCCCTCATC AGGCCTTATT GCGCCAGCAC CCTCCTCCAC ACCAGGAGTT TTTTTCTGAA 
CTGCGAGAAT GTGCTAAAGC AAAAGCCTTC TTCATAGATA CACGGTCCAA TAAAACACTG 
GCTGATTCTC TGGATAATGC GAATGACCCC AACGATCCCA TCGTAAACAG GTTGCTGCGA 
TCCATGGCCA CGCAGGCCAT GTCTAATGCA CTGTATTTCT CCACTGGGTC GTGTGCGGAG 
GAAGAGTTCC ATCATTATGG CCTTGCATTA GATAAATATA CTCACTTTAC ATCTCCGATC 
AGAAGATACT CAGATATAGT AGTACACCGA TTGTTAATGG CAGCCATTTC GAAAGATAAG 
AAAATGGAAA TTAAGGACAA TTTGTTCAGC AACAAAGATC TTGAGGAATT ATGCAGACAC 
ATCAACAACA GAAACCGAGC AGCACAGCAT TCTCAGAAGC AATCTACTGA GCTCTTCCAG 
TGCATGTATT TTAAAGACAA AGACCCAGAG AATGAGGAGC GTTGCATATC TGATGGGGTT 
ATATATTCAA TTAGAACAAA TGGTGTGCTT GTATTTATAC CAAGGTTTGG GATTAAAGGT 
GCTGCTTATT TAAGAAATAA AGATGGTTTG GTGATCTCAT GTGGCTCAGA CAGCCGTTCG 
GAATGGAAAC CGGGATCCCT TCAACGATTT CAAAACAAAA TCACCTCTAC CACAACAGGC 
GGGGAGTCTG TCACGTTCCA TCTGTTTGAC CATGTAACAG TGAGAATATC TGTACAGGCC 
TCCCGTTGCC ATTCTGATAC AATCAGGCTT GAAATAATAA GCAACAAACC GTACATGATA 
CCAGATACAG AACTTTTTCA TCAGAGTTCC CTCTTGTTAA AGAGTGATTT AGTGAAAGAA 
GTAACTAGAT CTGTGGAGGA AGCTCAGCTT GCCCAAGAAG TCAAAGTAAA CGTCATTCAG 
GAAGATTATC AAAAATACTG CCAAACAAAG GGAAGAAGCC TGTACACACT CCTAGAGGAG 
ATAAGGGATC TAGCTCTCCT GGATGTTTCC AACAGTTATG GAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021549583.1
CDS	27..3074
Translation	MTGSLLPDGKLLSSDVTHYMIPDWKVVQDYLEILEFPELKGIIFMQTACQTVQHQKGRRQYNKLRNLLKDARHDCILFANEFQQHCYLPRERGESMEKWQTRSIYNAAVWYFHHCQDRMPIVMVTEDKEAIQQYGSETEGVFVISFKNYLDNFWPDLKAAHELCDSILQSRRERENESQESHGKEYTEHLPLEVLEAGIKSGRYIQGILNVNKHRAQIEAFVRLQGASCKDSGLVSDILIHGMKARNRSIHGDVVVVELLPRNEWKGRTAALCENDSEDKALGDPLSEPMPTGRVVGILQKNWRDYVVTFPSKEEVQSQGKNAQKILVTPWDYRIPKIRISTQQAEALQDFRVVVRIDSWESTSVYPNGHFVRVLGRIGDLEGEIATILVENNISVVPFSEAQMCEMPVNTPGNPWKVSPEEERERKDLRETHLVFSIDPKGCEDVDDTLSVRTLTNGNLELGVHIADVTHFVPPNSYIDIEARTRATTYYLADRRYDMLPSILSADLCSLLGGVDRYAVSVIWELDKTSYEIKKVWYGRTIIRSAYKLFYEAAQELLDGNLHIVDDIPEFRDLDEKSRQAKLEELVWAISKLTDIARHIRAKRDHCGALELEGVEIRVQLDEKKNIHDLIPKQPLEVHETVAECMILANHWVAKKIWESFPHQALLRQHPPPHQEFFSELRECAKAKAFFIDTRSNKTLADSLDNANDPNDPIVNRLLRSMATQAMSNALYFSTGSCAEEEFHHYGLALDKYTHFTSPIRRYSDIVVHRLLMAAISKDKKMEIKDNLFSNKDLEELCRHINNRNRAAQHSQKQSTELFQCMYFKDKDPENEERCISDGVIYSIRTNGVLVFIPRFGIKGAAYLRNKDGLVISCGSDSRSEWKPGSLQRFQNKITSTTTGGESVTFHLFDHVTVRISVQASRCHSDTIRLEIISNKPYMIPDTELFHQSSLLLKSDLVKEVTRSVEEAQLAQEVKVNVIQEDYQKYCQTKGRSLYTLLEEIRDLALLDVSNSYGM

Target ORF information:

RefSeq Version	XM_021693908.1
Organism	Neomonachus schauinslandi(Hawaiian monk seal)
Definition	Neomonachus schauinslandi DIS3 like exosome 3'-5' exoribonuclease (DIS3L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021693908.1

ORF Insert Sequence:

ATGACTGGTT CTCTTCTGCC TGATGGGAAA CTCTTGTCTA GTGATGTGAC TCATTACATG 
ATCCCAGATT GGAAAGTTGT TCAAGATTAC CTGGAGATCC TTGAGTTCCC AGAGTTGAAG 
GGAATTATTT TCATGCAGAC GGCTTGTCAA ACTGTGCAGC ATCAAAAAGG CCGCAGACAG 
TATAACAAAT TGCGAAATCT CCTGAAGGAT GCACGTCATG ATTGCATTCT CTTTGCTAAT 
GAATTCCAGC AGCACTGCTA TCTCCCTCGA GAAAGAGGAG AATCCATGGA AAAGTGGCAG 
ACCAGGAGCA TATACAATGC AGCCGTGTGG TACTTTCACC ACTGCCAGGA TAGGATGCCA 
ATCGTTATGG TGACAGAAGA CAAAGAGGCA ATTCAGCAGT ATGGAAGTGA GACAGAAGGA 
GTGTTTGTGA TTTCTTTCAA GAATTACCTG GACAATTTTT GGCCCGACTT AAAGGCCGCC 
CACGAGCTCT GTGACTCTAT CCTTCAGTCT CGACGGGAAA GAGAGAATGA GAGTCAGGAG 
AGCCACGGGA AGGAGTACAC AGAACATCTT CCCCTGGAAG TGTTAGAAGC CGGCATTAAG 
TCTGGGCGCT ACATCCAGGG AATCCTGAAT GTCAACAAAC ACAGGGCACA AATAGAAGCT 
TTCGTTCGAC TTCAAGGAGC CAGCTGTAAA GATTCAGGTT TAGTCAGTGA CATCCTCATC 
CACGGGATGA AGGCTCGAAA CCGCTCGATC CATGGAGACG TGGTAGTCGT GGAATTGCTC 
CCTAGAAACG AATGGAAAGG AAGAACGGCT GCCCTGTGTG AGAATGACAG TGAGGACAAG 
GCCTTGGGAG ACCCCCTGAG TGAGCCCATG CCCACAGGCA GAGTGGTGGG CATCCTTCAG 
AAGAACTGGC GGGATTATGT GGTGACATTT CCGTCCAAAG AAGAGGTCCA GTCTCAGGGC 
AAAAACGCTC AGAAAATCCT AGTTACACCT TGGGATTACA GAATTCCCAA AATCCGCATT 
AGCACTCAGC AAGCAGAAGC GCTCCAGGAC TTCCGGGTGG TTGTACGCAT CGATTCCTGG 
GAGTCGACAT CTGTGTATCC AAATGGACAT TTTGTGCGTG TTTTAGGAAG AATCGGGGAC 
CTGGAAGGGG AAATCGCAAC CATCTTGGTG GAAAACAATA TTTCGGTTGT CCCTTTCTCC 
GAAGCTCAGA TGTGTGAGAT GCCAGTCAAC ACACCAGGAA ATCCCTGGAA AGTGAGTCCT 
GAAGAGGAAC GAGAACGGAA AGACTTGAGG GAAACCCATC TTGTGTTCAG CATTGACCCA 
AAAGGCTGTG AAGATGTGGA CGACACGCTC TCAGTCAGAA CCTTAACTAA TGGCAACTTG 
GAACTTGGGG TCCACATTGC AGACGTAACG CACTTCGTGC CACCAAATTC TTATATCGAC 
ATTGAAGCTA GGACAAGGGC CACCACTTAC TATTTAGCAG ACCGTCGCTA TGACATGCTG 
CCCTCCATCC TCAGCGCTGA TTTGTGCTCT CTTCTGGGAG GCGTTGATAG GTACGCCGTA 
AGTGTCATAT GGGAATTGGA TAAAACCTCC TATGAAATTA AGAAAGTGTG GTACGGCAGA 
ACCATTATTC GATCAGCATA CAAACTGTTT TATGAAGCAG CCCAGGAACT GCTGGACGGA 
AACCTCCACA TTGTTGATGA TATTCCAGAG TTCAGAGATT TGGATGAGAA GAGCAGACAA 
GCCAAGCTAG AGGAGTTAGT GTGGGCCATC AGCAAGTTGA CGGACATAGC GCGCCACATC 
CGGGCTAAGC GAGACCATTG CGGTGCCCTC GAACTGGAGG GGGTAGAGAT TCGTGTTCAG 
CTGGATGAGA AAAAGAACAT CCACGACCTC ATCCCCAAGC AGCCCCTGGA AGTCCACGAG 
ACGGTGGCCG AATGCATGAT CCTGGCCAAC CACTGGGTAG CCAAGAAGAT CTGGGAGAGC 
TTCCCTCATC AGGCCTTATT GCGCCAGCAC CCTCCTCCAC ACCAGGAGTT TTTTTCTGAA 
CTGCGAGAAT GTGCTAAAGC AAAAGCCTTC TTCATAGATA CACGGTCCAA TAAAACACTG 
GCTGATTCTC TGGATAATGC GAATGACCCC AACGATCCCA TCGTAAACAG GTTGCTGCGA 
TCCATGGCCA CGCAGGCCAT GTCTAATGCA CTGTATTTCT CCACTGGGTC GTGTGCGGAG 
GAAGAGTTCC ATCATTATGG CCTTGCATTA GATAAATATA CTCACTTTAC ATCTCCGATC 
AGAAGATACT CAGATATAGT AGTACACCGA TTGTTAATGG CAGCCATTTC GAAAGATAAG 
AAAATGGAAA TTAAGGACAA TTTGTTCAGC AACAAAGATC TTGAGGAATT ATGCAGACAC 
ATCAACAACA GAAACCGAGC AGCACAGCAT TCTCAGAAGC AATCTACTGA GCTCTTCCAG 
TGCATGTATT TTAAAGACAA AGACCCAGAG AATGAGGAGC GTTGCATATC TGATGGGGTT 
ATATATTCAA TTAGAACAAA TGGTGTGCTT GTATTTATAC CAAGGTTTGG GATTAAAGGT 
GCTGCTTATT TAAGAAATAA AGATGGTTTG GTGATCTCAT GTGGCTCAGA CAGCCGTTCG 
GAATGGAAAC CGGGATCCCT TCAACGATTT CAAAACAAAA TCACCTCTAC CACAACAGGC 
GGGGAGTCTG TCACGTTCCA TCTGTTTGAC CATGTAACAG TGAGAATATC TGTACAGGCC 
TCCCGTTGCC ATTCTGATAC AATCAGGCTT GAAATAATAA GCAACAAACC GTACATGATA 
CCAGATACAG AACTTTTTCA TCAGAGTTCC CTCTTGTTAA AGAGTGATTT AGTGAAAGAA 
GTAACTAGAT CTGTGGAGGA AGCTCAGCTT GCCCAAGAAG TCAAAGTAAA CGTCATTCAG 
GAAGATTATC AAAAATACTG CCAAACAAAG GGAAGAAGCC TGTACACACT CCTAGAGGAG 
ATAAGGGATC TAGCTCTCCT GGATGTTTCC AACAGTTATG GAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIS3L ( XM_021693909.1 ) cDNA ORF clone, Neomonachus schauinslandi(Hawaiian monk seal) -> XP_021549584.1 Neomonachus schauinslandi DIS3 like exosome 3'-5' exoribonuclease (DIS3L), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONe146400
Clone ID Related Accession (Same CDS sequence)	XM_021693909.1
Accession Version	XM_021693909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-27
Organism	Neomonachus schauinslandi(Hawaiian monk seal)
Product	DIS3-like exonuclease 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018734350.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Neomonachus schauinslandi Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGA AGCGGGAGAA GGTGCTGCTC CTGAGGACCT TCCAGGGCCG GACGCTGCGG 
ATCGTGCGGG AGCACTACCT TAGACCTTCG CCCACTTGCC AGGTTCGCAA TTGTTGCCTA 
ATCGCTTACA TTTTGAATTA TGTTCTAGAT GGGAAACTCT TGTCTAGTGA TGTGACTCAT 
TACATGATCC CAGATTGGAA AGTTGTTCAA GATTACCTGG AGATCCTTGA GTTCCCAGAG 
TTGAAGGGAA TTATTTTCAT GCAGACGGCT TGTCAAACTG TGCAGCATCA AAAAGGCCGC 
AGACAGTATA ACAAATTGCG AAATCTCCTG AAGGATGCAC GTCATGATTG CATTCTCTTT 
GCTAATGAAT TCCAGCAGCA CTGCTATCTC CCTCGAGAAA GAGGAGAATC CATGGAAAAG 
TGGCAGACCA GGAGCATATA CAATGCAGCC GTGTGGTACT TTCACCACTG CCAGGATAGG 
ATGCCAATCG TTATGGTGAC AGAAGACAAA GAGGCAATTC AGCAGTATGG AAGTGAGACA 
GAAGGAGTGT TTGTGATTTC TTTCAAGAAT TACCTGGACA ATTTTTGGCC CGACTTAAAG 
GCCGCCCACG AGCTCTGTGA CTCTATCCTT CAGTCTCGAC GGGAAAGAGA GAATGAGAGT 
CAGGAGAGCC ACGGGAAGGA GTACACAGAA CATCTTCCCC TGGAAGTGTT AGAAGCCGGC 
ATTAAGTCTG GGCGCTACAT CCAGGGAATC CTGAATGTCA ACAAACACAG GGCACAAATA 
GAAGCTTTCG TTCGACTTCA AGGAGCCAGC TGTAAAGATT CAGGTTTAGT CAGTGACATC 
CTCATCCACG GGATGAAGGC TCGAAACCGC TCGATCCATG GAGACGTGGT AGTCGTGGAA 
TTGCTCCCTA GAAACGAATG GAAAGGAAGA ACGGCTGCCC TGTGTGAGAA TGACAGTGAG 
GACAAGGCCT TGGGAGACCC CCTGAGTGAG CCCATGCCCA CAGGCAGAGT GGTGGGCATC 
CTTCAGAAGA ACTGGCGGGA TTATGTGGTG ACATTTCCGT CCAAAGAAGA GGTCCAGTCT 
CAGGGCAAAA ACGCTCAGAA AATCCTAGTT ACACCTTGGG ATTACAGAAT TCCCAAAATC 
CGCATTAGCA CTCAGCAAGC AGAAGCGCTC CAGGACTTCC GGGTGGTTGT ACGCATCGAT 
TCCTGGGAGT CGACATCTGT GTATCCAAAT GGACATTTTG TGCGTGTTTT AGGAAGAATC 
GGGGACCTGG AAGGGGAAAT CGCAACCATC TTGGTGGAAA ACAATATTTC GGTTGTCCCT 
TTCTCCGAAG CTCAGATGTG TGAGATGCCA GTCAACACAC CAGGAAATCC CTGGAAAGTG 
AGTCCTGAAG AGGAACGAGA ACGGAAAGAC TTGAGGGAAA CCCATCTTGT GTTCAGCATT 
GACCCAAAAG GCTGTGAAGA TGTGGACGAC ACGCTCTCAG TCAGAACCTT AACTAATGGC 
AACTTGGAAC TTGGGGTCCA CATTGCAGAC GTAACGCACT TCGTGCCACC AAATTCTTAT 
ATCGACATTG AAGCTAGGAC AAGGGCCACC ACTTACTATT TAGCAGACCG TCGCTATGAC 
ATGCTGCCCT CCATCCTCAG CGCTGATTTG TGCTCTCTTC TGGGAGGCGT TGATAGGTAC 
GCCGTAAGTG TCATATGGGA ATTGGATAAA ACCTCCTATG AAATTAAGAA AGTGTGGTAC 
GGCAGAACCA TTATTCGATC AGCATACAAA CTGTTTTATG AAGCAGCCCA GGAACTGCTG 
GACGGAAACC TCCACATTGT TGATGATATT CCAGAGTTCA GAGATTTGGA TGAGAAGAGC 
AGACAAGCCA AGCTAGAGGA GTTAGTGTGG GCCATCAGCA AGTTGACGGA CATAGCGCGC 
CACATCCGGG CTAAGCGAGA CCATTGCGGT GCCCTCGAAC TGGAGGGGGT AGAGATTCGT 
GTTCAGCTGG ATGAGAAAAA GAACATCCAC GACCTCATCC CCAAGCAGCC CCTGGAAGTC 
CACGAGACGG TGGCCGAATG CATGATCCTG GCCAACCACT GGGTAGCCAA GAAGATCTGG 
GAGAGCTTCC CTCATCAGGC CTTATTGCGC CAGCACCCTC CTCCACACCA GGAGTTTTTT 
TCTGAACTGC GAGAATGTGC TAAAGCAAAA GCCTTCTTCA TAGATACACG GTCCAATAAA 
ACACTGGCTG ATTCTCTGGA TAATGCGAAT GACCCCAACG ATCCCATCGT AAACAGGTTG 
CTGCGATCCA TGGCCACGCA GGCCATGTCT AATGCACTGT ATTTCTCCAC TGGGTCGTGT 
GCGGAGGAAG AGTTCCATCA TTATGGCCTT GCATTAGATA AATATACTCA CTTTACATCT 
CCGATCAGAA GATACTCAGA TATAGTAGTA CACCGATTGT TAATGGCAGC CATTTCGAAA 
GATAAGAAAA TGGAAATTAA GGACAATTTG TTCAGCAACA AAGATCTTGA GGAATTATGC 
AGACACATCA ACAACAGAAA CCGAGCAGCA CAGCATTCTC AGAAGCAATC TACTGAGCTC 
TTCCAGTGCA TGTATTTTAA AGACAAAGAC CCAGAGAATG AGGAGCGTTG CATATCTGAT 
GGGGTTATAT ATTCAATTAG AACAAATGGT GTGCTTGTAT TTATACCAAG GTTTGGGATT 
AAAGGTGCTG CTTATTTAAG AAATAAAGAT GGTTTGGTGA TCTCATGTGG CTCAGACAGC 
CGTTCGGAAT GGAAACCGGG ATCCCTTCAA CGATTTCAAA ACAAAATCAC CTCTACCACA 
ACAGGCGGGG AGTCTGTCAC GTTCCATCTG TTTGACCATG TAACAGTGAG AATATCTGTA 
CAGGCCTCCC GTTGCCATTC TGATACAATC AGGCTTGAAA TAATAAGCAA CAAACCGTAC 
ATGATACCAG ATACAGAACT TTTTCATCAG AGTTCCCTCT TGTTAAAGAG TGATTTAGTG 
AAAGAAGTAA CTAGATCTGT GGAGGAAGCT CAGCTTGCCC AAGAAGTCAA AGTAAACGTC 
ATTCAGGAAG ATTATCAAAA ATACTGCCAA ACAAAGGGAA GAAGCCTGTA CACACTCCTA 
GAGGAGATAA GGGATCTAGC TCTCCTGGAT GTTTCCAACA GTTATGGAAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021549584.1
CDS	46..3219
Translation	MLQKREKVLLLRTFQGRTLRIVREHYLRPSPTCQVRNCCLIAYILNYVLDGKLLSSDVTHYMIPDWKVVQDYLEILEFPELKGIIFMQTACQTVQHQKGRRQYNKLRNLLKDARHDCILFANEFQQHCYLPRERGESMEKWQTRSIYNAAVWYFHHCQDRMPIVMVTEDKEAIQQYGSETEGVFVISFKNYLDNFWPDLKAAHELCDSILQSRRERENESQESHGKEYTEHLPLEVLEAGIKSGRYIQGILNVNKHRAQIEAFVRLQGASCKDSGLVSDILIHGMKARNRSIHGDVVVVELLPRNEWKGRTAALCENDSEDKALGDPLSEPMPTGRVVGILQKNWRDYVVTFPSKEEVQSQGKNAQKILVTPWDYRIPKIRISTQQAEALQDFRVVVRIDSWESTSVYPNGHFVRVLGRIGDLEGEIATILVENNISVVPFSEAQMCEMPVNTPGNPWKVSPEEERERKDLRETHLVFSIDPKGCEDVDDTLSVRTLTNGNLELGVHIADVTHFVPPNSYIDIEARTRATTYYLADRRYDMLPSILSADLCSLLGGVDRYAVSVIWELDKTSYEIKKVWYGRTIIRSAYKLFYEAAQELLDGNLHIVDDIPEFRDLDEKSRQAKLEELVWAISKLTDIARHIRAKRDHCGALELEGVEIRVQLDEKKNIHDLIPKQPLEVHETVAECMILANHWVAKKIWESFPHQALLRQHPPPHQEFFSELRECAKAKAFFIDTRSNKTLADSLDNANDPNDPIVNRLLRSMATQAMSNALYFSTGSCAEEEFHHYGLALDKYTHFTSPIRRYSDIVVHRLLMAAISKDKKMEIKDNLFSNKDLEELCRHINNRNRAAQHSQKQSTELFQCMYFKDKDPENEERCISDGVIYSIRTNGVLVFIPRFGIKGAAYLRNKDGLVISCGSDSRSEWKPGSLQRFQNKITSTTTGGESVTFHLFDHVTVRISVQASRCHSDTIRLEIISNKPYMIPDTELFHQSSLLLKSDLVKEVTRSVEEAQLAQEVKVNVIQEDYQKYCQTKGRSLYTLLEEIRDLALLDVSNSYGM

Target ORF information:

RefSeq Version	XM_021693909.1
Organism	Neomonachus schauinslandi(Hawaiian monk seal)
Definition	Neomonachus schauinslandi DIS3 like exosome 3'-5' exoribonuclease (DIS3L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021693909.1

ORF Insert Sequence:

ATGCTGCAGA AGCGGGAGAA GGTGCTGCTC CTGAGGACCT TCCAGGGCCG GACGCTGCGG 
ATCGTGCGGG AGCACTACCT TAGACCTTCG CCCACTTGCC AGGTTCGCAA TTGTTGCCTA 
ATCGCTTACA TTTTGAATTA TGTTCTAGAT GGGAAACTCT TGTCTAGTGA TGTGACTCAT 
TACATGATCC CAGATTGGAA AGTTGTTCAA GATTACCTGG AGATCCTTGA GTTCCCAGAG 
TTGAAGGGAA TTATTTTCAT GCAGACGGCT TGTCAAACTG TGCAGCATCA AAAAGGCCGC 
AGACAGTATA ACAAATTGCG AAATCTCCTG AAGGATGCAC GTCATGATTG CATTCTCTTT 
GCTAATGAAT TCCAGCAGCA CTGCTATCTC CCTCGAGAAA GAGGAGAATC CATGGAAAAG 
TGGCAGACCA GGAGCATATA CAATGCAGCC GTGTGGTACT TTCACCACTG CCAGGATAGG 
ATGCCAATCG TTATGGTGAC AGAAGACAAA GAGGCAATTC AGCAGTATGG AAGTGAGACA 
GAAGGAGTGT TTGTGATTTC TTTCAAGAAT TACCTGGACA ATTTTTGGCC CGACTTAAAG 
GCCGCCCACG AGCTCTGTGA CTCTATCCTT CAGTCTCGAC GGGAAAGAGA GAATGAGAGT 
CAGGAGAGCC ACGGGAAGGA GTACACAGAA CATCTTCCCC TGGAAGTGTT AGAAGCCGGC 
ATTAAGTCTG GGCGCTACAT CCAGGGAATC CTGAATGTCA ACAAACACAG GGCACAAATA 
GAAGCTTTCG TTCGACTTCA AGGAGCCAGC TGTAAAGATT CAGGTTTAGT CAGTGACATC 
CTCATCCACG GGATGAAGGC TCGAAACCGC TCGATCCATG GAGACGTGGT AGTCGTGGAA 
TTGCTCCCTA GAAACGAATG GAAAGGAAGA ACGGCTGCCC TGTGTGAGAA TGACAGTGAG 
GACAAGGCCT TGGGAGACCC CCTGAGTGAG CCCATGCCCA CAGGCAGAGT GGTGGGCATC 
CTTCAGAAGA ACTGGCGGGA TTATGTGGTG ACATTTCCGT CCAAAGAAGA GGTCCAGTCT 
CAGGGCAAAA ACGCTCAGAA AATCCTAGTT ACACCTTGGG ATTACAGAAT TCCCAAAATC 
CGCATTAGCA CTCAGCAAGC AGAAGCGCTC CAGGACTTCC GGGTGGTTGT ACGCATCGAT 
TCCTGGGAGT CGACATCTGT GTATCCAAAT GGACATTTTG TGCGTGTTTT AGGAAGAATC 
GGGGACCTGG AAGGGGAAAT CGCAACCATC TTGGTGGAAA ACAATATTTC GGTTGTCCCT 
TTCTCCGAAG CTCAGATGTG TGAGATGCCA GTCAACACAC CAGGAAATCC CTGGAAAGTG 
AGTCCTGAAG AGGAACGAGA ACGGAAAGAC TTGAGGGAAA CCCATCTTGT GTTCAGCATT 
GACCCAAAAG GCTGTGAAGA TGTGGACGAC ACGCTCTCAG TCAGAACCTT AACTAATGGC 
AACTTGGAAC TTGGGGTCCA CATTGCAGAC GTAACGCACT TCGTGCCACC AAATTCTTAT 
ATCGACATTG AAGCTAGGAC AAGGGCCACC ACTTACTATT TAGCAGACCG TCGCTATGAC 
ATGCTGCCCT CCATCCTCAG CGCTGATTTG TGCTCTCTTC TGGGAGGCGT TGATAGGTAC 
GCCGTAAGTG TCATATGGGA ATTGGATAAA ACCTCCTATG AAATTAAGAA AGTGTGGTAC 
GGCAGAACCA TTATTCGATC AGCATACAAA CTGTTTTATG AAGCAGCCCA GGAACTGCTG 
GACGGAAACC TCCACATTGT TGATGATATT CCAGAGTTCA GAGATTTGGA TGAGAAGAGC 
AGACAAGCCA AGCTAGAGGA GTTAGTGTGG GCCATCAGCA AGTTGACGGA CATAGCGCGC 
CACATCCGGG CTAAGCGAGA CCATTGCGGT GCCCTCGAAC TGGAGGGGGT AGAGATTCGT 
GTTCAGCTGG ATGAGAAAAA GAACATCCAC GACCTCATCC CCAAGCAGCC CCTGGAAGTC 
CACGAGACGG TGGCCGAATG CATGATCCTG GCCAACCACT GGGTAGCCAA GAAGATCTGG 
GAGAGCTTCC CTCATCAGGC CTTATTGCGC CAGCACCCTC CTCCACACCA GGAGTTTTTT 
TCTGAACTGC GAGAATGTGC TAAAGCAAAA GCCTTCTTCA TAGATACACG GTCCAATAAA 
ACACTGGCTG ATTCTCTGGA TAATGCGAAT GACCCCAACG ATCCCATCGT AAACAGGTTG 
CTGCGATCCA TGGCCACGCA GGCCATGTCT AATGCACTGT ATTTCTCCAC TGGGTCGTGT 
GCGGAGGAAG AGTTCCATCA TTATGGCCTT GCATTAGATA AATATACTCA CTTTACATCT 
CCGATCAGAA GATACTCAGA TATAGTAGTA CACCGATTGT TAATGGCAGC CATTTCGAAA 
GATAAGAAAA TGGAAATTAA GGACAATTTG TTCAGCAACA AAGATCTTGA GGAATTATGC 
AGACACATCA ACAACAGAAA CCGAGCAGCA CAGCATTCTC AGAAGCAATC TACTGAGCTC 
TTCCAGTGCA TGTATTTTAA AGACAAAGAC CCAGAGAATG AGGAGCGTTG CATATCTGAT 
GGGGTTATAT ATTCAATTAG AACAAATGGT GTGCTTGTAT TTATACCAAG GTTTGGGATT 
AAAGGTGCTG CTTATTTAAG AAATAAAGAT GGTTTGGTGA TCTCATGTGG CTCAGACAGC 
CGTTCGGAAT GGAAACCGGG ATCCCTTCAA CGATTTCAAA ACAAAATCAC CTCTACCACA 
ACAGGCGGGG AGTCTGTCAC GTTCCATCTG TTTGACCATG TAACAGTGAG AATATCTGTA 
CAGGCCTCCC GTTGCCATTC TGATACAATC AGGCTTGAAA TAATAAGCAA CAAACCGTAC 
ATGATACCAG ATACAGAACT TTTTCATCAG AGTTCCCTCT TGTTAAAGAG TGATTTAGTG 
AAAGAAGTAA CTAGATCTGT GGAGGAAGCT CAGCTTGCCC AAGAAGTCAA AGTAAACGTC 
ATTCAGGAAG ATTATCAAAA ATACTGCCAA ACAAAGGGAA GAAGCCTGTA CACACTCCTA 
GAGGAGATAA GGGATCTAGC TCTCCTGGAT GTTTCCAACA GTTATGGAAT GTGA