LOC104594089 cDNA ORF clone, Nelumbo nucifera(sacred lotus)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104594089
Entrez Gene ID	104594089
Full Name	U-box domain-containing protein 44-like
Gene Type	protein-coding
Organism	Nelumbo nucifera(sacred lotus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010254239.2	XP_010252541.1	U-box domain-containing protein 44-like isoform X2
XM_010254232.2	XP_010252534.1	U-box domain-containing protein 44-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC104594089 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104594089 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe25079	XM_010254239.2 Latest version!	Nelumbo nucifera U-box domain-containing protein 44-like (LOC104594089), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ONe25078	XM_010254232.2 Latest version!	Nelumbo nucifera U-box domain-containing protein 44-like (LOC104594089), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104594089 ( XM_010254239.2 ) cDNA ORF clone, Nelumbo nucifera(sacred lotus) -> XP_010252541.1 Nelumbo nucifera U-box domain-containing protein 44-like (LOC104594089), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONe25079
Clone ID Related Accession (Same CDS sequence)	XM_010254239.2
Accession Version	XM_010254239.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-15
Organism	Nelumbo nucifera(sacred lotus)
Product	U-box domain-containing protein 44-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010729074.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 16, 2016 this sequence version replaced XM_010254239.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nelumbo nucifera Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTT TTGCATTAGC AGAATTACTT GATCGGATAA TAGAGACGAT AGCCGAGACT 
GCAGAGGCTG CTAAGGATGT GCTTGTTGAG AAGGAAGGTT TTGCAAAGCT GTCGAGTTTC 
TTGGAAAAGG TTGTTCCTAT CCTAAAAGAA TTGACCAAGA AGAACATCAG TAACTCTGAG 
AGCCTAAATA ATGCCATTGA AAATTTCGAT CGGGAGGTCA AAGCTGCGAA GCAGCTAGTT 
CTGGAGTGCA GAAAGAGAAA CAAAGTCTAT CTCTTTCTCA ATTGCCGGAG AGTAGTGAAG 
CGTTTAGAGC AAACTACGAG AGAGATCAGC CGGGCTCTGA GCTTTATCCC TTTGGCATCA 
CTAGATCTCT CATCTAGTAT AAATGAGGAT ATCAGCAACC TATGTGACAA CATGCAGAGG 
GCAGAGTTTA AGGCAGCTGT AGCAGAGGAA GAGATATTGG AGAAAATTGA ATTGGGTATA 
TTAGAGAGAA ATGTTGATCG TACTTACGCA AACAATTTGA TGGTTCTCAT TGCTGAAGCT 
GTTGGGATAT CAACTGAACG CTCAGCACTG AAGAAGGAAT TTGAAGATTT CAAAAGAGAA 
ATAGAAGACA CGCAGGTGAG GAAAGACCAG GCCGAGGCTA TACAAATGGA TCAGATCGTT 
GCATTGCTTG AAAGAGCTGA TGCCACTTCA TCCCCTAGGG AAAAAGAGAT GAAATACTTC 
ACCAAGAGGA AGTCTTTAGG TAATCAGCCA TTGGAACCTC TCCAGTCATT TTATTGCCCA 
ATCACTCGAG ATGTTATGGT GGACCCTGTT GAGACCTCCT CTGGACAGAC ATTCGAGAGA 
GATGCAATTC AGAAGTGGTT TGCAGATGGG AACACCATTT GCCCCCTAAC CATGATCCCC 
TTAGACACAG CAATTTTACG GCCCAACAAA ACTCTACGGC AATCAATTGA AGAATGGAGG 
GATAGAAACA TCATGATTAC AATTGCTTCC ATGAAACCCA AACTCCATTC AGATGATGAA 
CAAGAAATTC TTCATTCCTT GGAACAACTG CAGGATCTTT GCATGGAGAG AGACTTGCAC 
CGAGAATGGG TGACACTGGA GAACTACATA CCAATTCTTA TTGGATTGTT ATCCACAAAA 
AATCGTGAGA TAAGGAACCA TGTTTTAGAC ATCCTTTGCA TACTGGCAAA GGATAGTGAT 
GACACTAAGG AAAGAATTGC TGAAGTGGAA AATGCAATTG AAGCTATTGT GCGATCCCTT 
GCTCGTCGTA TTAGTGAAAG TAAGCTTGCT GTGGCATTGC TGTTGGAACT TTCAAAAAGT 
AATATGGTAC GTAACTACAT TGGGAAAGTA CAGGGATGTA TACTCCTCCT GGTGACTATG 
TCAACGAGTG ATGACACTCA AGCTGCCAAA GATGCAAAGG AACTCTTGGA GAATCTTTCA 
TTCCTTGATG AAAATGTTGT ACAAATGGCC AAGGCAAATT ATTTCAGGCC TTTGTTGGAG 
CGCCTTTCTT CAGGGCCAGA AGATGTCAAG ATGATCATGT TGACCACTCT GGCAGAAATG 
GAGTTGAGTG ACCACAATAA ATTGACTTTG TTTGAGAATG GAGTACTGAA GCCACTTCTT 
CACTTGGTCA CAAATGGCAA CGAGGAGATG AAGAGAGTTT CTGTTAAAGC TATTCAGAAT 
CTTTCCACTT TACCAAGGAA TGCCTTGCAG ATGATTCAGG AAGGTGCAGT GGGTCCACTA 
CTTGACCTAC TCTACCGTCA TAATGCATCC ATGATTTTGC GAGAACAAGT AGCATCAACC 
ATCATGAACC TTGCGATATC AACAGCGAAA CAAGAAGCCA AACAGACAGA AGTGACACTA 
CTTGAATATG ATGAAGACAT TTTCAAACTC TTCTCCTTGG TCAACTTGAC AGCACCTACT 
ATACAACAGA AGATACTGAG GACCTTTCAT GCTATGTGCC AACACCCATC TACAATAGAC 
ATAAGGTCTA AACTTAGACA GTGCTCGGCC ATTCAAATGT TGGTTCAATT TTGTGAGGTT 
GATGATATTG CTCTACGAGC AAATGCTTTA AAGCTGTTCT TCTGCTTGAC GGAAGATGGA 
GATGATGGCT CGTTGTCAGA ATATGTGGAC CAGAGGTGCA TTGGGACCTT GATCAGGATC 
ATCAGAGATT GTCATGATGA GGAAGAAATT ACTGCTGCTC TGGGTATTAT CTCCAACCTT 
CCAATGAAGA ACATCCAAAT CACCCAGTGG CTTCTAGATG CTGAGGCACT TCCAGTAATT 
TTCAGGTTCC TAACTAATGG AAAATTTAAT GGATCCTATA AAAACGATCT AATAGAGAAT 
GCAGTTGGAG CAACATGCCG CTTCACTGTA TCAACAAATC TGGAGTGGCA GAAGAGAGCA 
GCTGAAGCTG GCATTATCCC AGTGCTAGTA CAACTGCTAA GCTCTGGAAC TGCTTTGACA 
AAACAACATG CGGCCACTTC CCTTGCCCAG TTCTCAGAGA GTTCAGTTAG GTTGAGTAGG 
CCAATCAAGA GACATAGGGG GCTCTGGTGC TGCTCACCAC CACCTGAAGT TGGCTGCCCA 
GTGCACATGG GCATATGTAC AGTTGAGTTA TCATTTTGCC TTGTCGAGGC TGGAGCTGTG 
CAACCCCTAG TAACTATACT TGGAGAATCA GATTTTGGAG CCTCCAAGGC TTCTTTACAG 
GCATTGTTGA CACTGATTGA TGGTGAAAGA CTGCAGAGTG GTAGTAAGGT GCTTGCTGAA 
GCAAATGCCA TTCCACCAAT TATTAAGTTA TTAGGCTCCC CTTCTACTGA ATTGCAGGAA 
AGGGCTCTTC TTGCTTTGGA AAGAATATTC CGGTTGATAG AATTCAAACA AAAGTACGGA 
GCATCAGCTC AGATGCCTTT AGTTGACTTA ACTCAGAGGG GAAACAGTAC TACAAAACCT 
TTGGCAGCAC GGATTCTTGC ACACTTGAAT GTACTTCACG AGTCATCATC TTATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010252541.1
CDS	399..3398
Translation	MDIFALAELLDRIIETIAETAEAAKDVLVEKEGFAKLSSFLEKVVPILKELTKKNISNSESLNNAIENFDREVKAAKQLVLECRKRNKVYLFLNCRRVVKRLEQTTREISRALSFIPLASLDLSSSINEDISNLCDNMQRAEFKAAVAEEEILEKIELGILERNVDRTYANNLMVLIAEAVGISTERSALKKEFEDFKREIEDTQVRKDQAEAIQMDQIVALLERADATSSPREKEMKYFTKRKSLGNQPLEPLQSFYCPITRDVMVDPVETSSGQTFERDAIQKWFADGNTICPLTMIPLDTAILRPNKTLRQSIEEWRDRNIMITIASMKPKLHSDDEQEILHSLEQLQDLCMERDLHREWVTLENYIPILIGLLSTKNREIRNHVLDILCILAKDSDDTKERIAEVENAIEAIVRSLARRISESKLAVALLLELSKSNMVRNYIGKVQGCILLLVTMSTSDDTQAAKDAKELLENLSFLDENVVQMAKANYFRPLLERLSSGPEDVKMIMLTTLAEMELSDHNKLTLFENGVLKPLLHLVTNGNEEMKRVSVKAIQNLSTLPRNALQMIQEGAVGPLLDLLYRHNASMILREQVASTIMNLAISTAKQEAKQTEVTLLEYDEDIFKLFSLVNLTAPTIQQKILRTFHAMCQHPSTIDIRSKLRQCSAIQMLVQFCEVDDIALRANALKLFFCLTEDGDDGSLSEYVDQRCIGTLIRIIRDCHDEEEITAALGIISNLPMKNIQITQWLLDAEALPVIFRFLTNGKFNGSYKNDLIENAVGATCRFTVSTNLEWQKRAAEAGIIPVLVQLLSSGTALTKQHAATSLAQFSESSVRLSRPIKRHRGLWCCSPPPEVGCPVHMGICTVELSFCLVEAGAVQPLVTILGESDFGASKASLQALLTLIDGERLQSGSKVLAEANAIPPIIKLLGSPSTELQERALLALERIFRLIEFKQKYGASAQMPLVDLTQRGNSTTKPLAARILAHLNVLHESSSYF

Target ORF information:

RefSeq Version	XM_010254239.2
Organism	Nelumbo nucifera(sacred lotus)
Definition	Nelumbo nucifera U-box domain-containing protein 44-like (LOC104594089), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010254239.2

ORF Insert Sequence:

ATGGATATTT TTGCATTAGC AGAATTACTT GATCGGATAA TAGAGACGAT AGCCGAGACT 
GCAGAGGCTG CTAAGGATGT GCTTGTTGAG AAGGAAGGTT TTGCAAAGCT GTCGAGTTTC 
TTGGAAAAGG TTGTTCCTAT CCTAAAAGAA TTGACCAAGA AGAACATCAG TAACTCTGAG 
AGCCTAAATA ATGCCATTGA AAATTTCGAT CGGGAGGTCA AAGCTGCGAA GCAGCTAGTT 
CTGGAGTGCA GAAAGAGAAA CAAAGTCTAT CTCTTTCTCA ATTGCCGGAG AGTAGTGAAG 
CGTTTAGAGC AAACTACGAG AGAGATCAGC CGGGCTCTGA GCTTTATCCC TTTGGCATCA 
CTAGATCTCT CATCTAGTAT AAATGAGGAT ATCAGCAACC TATGTGACAA CATGCAGAGG 
GCAGAGTTTA AGGCAGCTGT AGCAGAGGAA GAGATATTGG AGAAAATTGA ATTGGGTATA 
TTAGAGAGAA ATGTTGATCG TACTTACGCA AACAATTTGA TGGTTCTCAT TGCTGAAGCT 
GTTGGGATAT CAACTGAACG CTCAGCACTG AAGAAGGAAT TTGAAGATTT CAAAAGAGAA 
ATAGAAGACA CGCAGGTGAG GAAAGACCAG GCCGAGGCTA TACAAATGGA TCAGATCGTT 
GCATTGCTTG AAAGAGCTGA TGCCACTTCA TCCCCTAGGG AAAAAGAGAT GAAATACTTC 
ACCAAGAGGA AGTCTTTAGG TAATCAGCCA TTGGAACCTC TCCAGTCATT TTATTGCCCA 
ATCACTCGAG ATGTTATGGT GGACCCTGTT GAGACCTCCT CTGGACAGAC ATTCGAGAGA 
GATGCAATTC AGAAGTGGTT TGCAGATGGG AACACCATTT GCCCCCTAAC CATGATCCCC 
TTAGACACAG CAATTTTACG GCCCAACAAA ACTCTACGGC AATCAATTGA AGAATGGAGG 
GATAGAAACA TCATGATTAC AATTGCTTCC ATGAAACCCA AACTCCATTC AGATGATGAA 
CAAGAAATTC TTCATTCCTT GGAACAACTG CAGGATCTTT GCATGGAGAG AGACTTGCAC 
CGAGAATGGG TGACACTGGA GAACTACATA CCAATTCTTA TTGGATTGTT ATCCACAAAA 
AATCGTGAGA TAAGGAACCA TGTTTTAGAC ATCCTTTGCA TACTGGCAAA GGATAGTGAT 
GACACTAAGG AAAGAATTGC TGAAGTGGAA AATGCAATTG AAGCTATTGT GCGATCCCTT 
GCTCGTCGTA TTAGTGAAAG TAAGCTTGCT GTGGCATTGC TGTTGGAACT TTCAAAAAGT 
AATATGGTAC GTAACTACAT TGGGAAAGTA CAGGGATGTA TACTCCTCCT GGTGACTATG 
TCAACGAGTG ATGACACTCA AGCTGCCAAA GATGCAAAGG AACTCTTGGA GAATCTTTCA 
TTCCTTGATG AAAATGTTGT ACAAATGGCC AAGGCAAATT ATTTCAGGCC TTTGTTGGAG 
CGCCTTTCTT CAGGGCCAGA AGATGTCAAG ATGATCATGT TGACCACTCT GGCAGAAATG 
GAGTTGAGTG ACCACAATAA ATTGACTTTG TTTGAGAATG GAGTACTGAA GCCACTTCTT 
CACTTGGTCA CAAATGGCAA CGAGGAGATG AAGAGAGTTT CTGTTAAAGC TATTCAGAAT 
CTTTCCACTT TACCAAGGAA TGCCTTGCAG ATGATTCAGG AAGGTGCAGT GGGTCCACTA 
CTTGACCTAC TCTACCGTCA TAATGCATCC ATGATTTTGC GAGAACAAGT AGCATCAACC 
ATCATGAACC TTGCGATATC AACAGCGAAA CAAGAAGCCA AACAGACAGA AGTGACACTA 
CTTGAATATG ATGAAGACAT TTTCAAACTC TTCTCCTTGG TCAACTTGAC AGCACCTACT 
ATACAACAGA AGATACTGAG GACCTTTCAT GCTATGTGCC AACACCCATC TACAATAGAC 
ATAAGGTCTA AACTTAGACA GTGCTCGGCC ATTCAAATGT TGGTTCAATT TTGTGAGGTT 
GATGATATTG CTCTACGAGC AAATGCTTTA AAGCTGTTCT TCTGCTTGAC GGAAGATGGA 
GATGATGGCT CGTTGTCAGA ATATGTGGAC CAGAGGTGCA TTGGGACCTT GATCAGGATC 
ATCAGAGATT GTCATGATGA GGAAGAAATT ACTGCTGCTC TGGGTATTAT CTCCAACCTT 
CCAATGAAGA ACATCCAAAT CACCCAGTGG CTTCTAGATG CTGAGGCACT TCCAGTAATT 
TTCAGGTTCC TAACTAATGG AAAATTTAAT GGATCCTATA AAAACGATCT AATAGAGAAT 
GCAGTTGGAG CAACATGCCG CTTCACTGTA TCAACAAATC TGGAGTGGCA GAAGAGAGCA 
GCTGAAGCTG GCATTATCCC AGTGCTAGTA CAACTGCTAA GCTCTGGAAC TGCTTTGACA 
AAACAACATG CGGCCACTTC CCTTGCCCAG TTCTCAGAGA GTTCAGTTAG GTTGAGTAGG 
CCAATCAAGA GACATAGGGG GCTCTGGTGC TGCTCACCAC CACCTGAAGT TGGCTGCCCA 
GTGCACATGG GCATATGTAC AGTTGAGTTA TCATTTTGCC TTGTCGAGGC TGGAGCTGTG 
CAACCCCTAG TAACTATACT TGGAGAATCA GATTTTGGAG CCTCCAAGGC TTCTTTACAG 
GCATTGTTGA CACTGATTGA TGGTGAAAGA CTGCAGAGTG GTAGTAAGGT GCTTGCTGAA 
GCAAATGCCA TTCCACCAAT TATTAAGTTA TTAGGCTCCC CTTCTACTGA ATTGCAGGAA 
AGGGCTCTTC TTGCTTTGGA AAGAATATTC CGGTTGATAG AATTCAAACA AAAGTACGGA 
GCATCAGCTC AGATGCCTTT AGTTGACTTA ACTCAGAGGG GAAACAGTAC TACAAAACCT 
TTGGCAGCAC GGATTCTTGC ACACTTGAAT GTACTTCACG AGTCATCATC TTATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104594089 ( XM_010254232.2 ) cDNA ORF clone, Nelumbo nucifera(sacred lotus) -> XP_010252534.1 Nelumbo nucifera U-box domain-containing protein 44-like (LOC104594089), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONe25078
Clone ID Related Accession (Same CDS sequence)	XM_010254232.2
Accession Version	XM_010254232.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-15
Organism	Nelumbo nucifera(sacred lotus)
Product	U-box domain-containing protein 44-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010729074.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 16, 2016 this sequence version replaced XM_010254232.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nelumbo nucifera Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTTA CTAGCTTATT TTTCCTGCTA AAAGAGATGG CAACAAATAC CATGATTAAT 
GTTTCACTTG TTCCGGCTTC AGAATTACTT GATCGGATAA TAGAGACGAT AGCCGAGACT 
GCAGAGGCTG CTAAGGATGT GCTTGTTGAG AAGGAAGGTT TTGCAAAGCT GTCGAGTTTC 
TTGGAAAAGG TTGTTCCTAT CCTAAAAGAA TTGACCAAGA AGAACATCAG TAACTCTGAG 
AGCCTAAATA ATGCCATTGA AAATTTCGAT CGGGAGGTCA AAGCTGCGAA GCAGCTAGTT 
CTGGAGTGCA GAAAGAGAAA CAAAGTCTAT CTCTTTCTCA ATTGCCGGAG AGTAGTGAAG 
CGTTTAGAGC AAACTACGAG AGAGATCAGC CGGGCTCTGA GCTTTATCCC TTTGGCATCA 
CTAGATCTCT CATCTAGTAT AAATGAGGAT ATCAGCAACC TATGTGACAA CATGCAGAGG 
GCAGAGTTTA AGGCAGCTGT AGCAGAGGAA GAGATATTGG AGAAAATTGA ATTGGGTATA 
TTAGAGAGAA ATGTTGATCG TACTTACGCA AACAATTTGA TGGTTCTCAT TGCTGAAGCT 
GTTGGGATAT CAACTGAACG CTCAGCACTG AAGAAGGAAT TTGAAGATTT CAAAAGAGAA 
ATAGAAGACA CGCAGGTGAG GAAAGACCAG GCCGAGGCTA TACAAATGGA TCAGATCGTT 
GCATTGCTTG AAAGAGCTGA TGCCACTTCA TCCCCTAGGG AAAAAGAGAT GAAATACTTC 
ACCAAGAGGA AGTCTTTAGG TAATCAGCCA TTGGAACCTC TCCAGTCATT TTATTGCCCA 
ATCACTCGAG ATGTTATGGT GGACCCTGTT GAGACCTCCT CTGGACAGAC ATTCGAGAGA 
GATGCAATTC AGAAGTGGTT TGCAGATGGG AACACCATTT GCCCCCTAAC CATGATCCCC 
TTAGACACAG CAATTTTACG GCCCAACAAA ACTCTACGGC AATCAATTGA AGAATGGAGG 
GATAGAAACA TCATGATTAC AATTGCTTCC ATGAAACCCA AACTCCATTC AGATGATGAA 
CAAGAAATTC TTCATTCCTT GGAACAACTG CAGGATCTTT GCATGGAGAG AGACTTGCAC 
CGAGAATGGG TGACACTGGA GAACTACATA CCAATTCTTA TTGGATTGTT ATCCACAAAA 
AATCGTGAGA TAAGGAACCA TGTTTTAGAC ATCCTTTGCA TACTGGCAAA GGATAGTGAT 
GACACTAAGG AAAGAATTGC TGAAGTGGAA AATGCAATTG AAGCTATTGT GCGATCCCTT 
GCTCGTCGTA TTAGTGAAAG TAAGCTTGCT GTGGCATTGC TGTTGGAACT TTCAAAAAGT 
AATATGGTAC GTAACTACAT TGGGAAAGTA CAGGGATGTA TACTCCTCCT GGTGACTATG 
TCAACGAGTG ATGACACTCA AGCTGCCAAA GATGCAAAGG AACTCTTGGA GAATCTTTCA 
TTCCTTGATG AAAATGTTGT ACAAATGGCC AAGGCAAATT ATTTCAGGCC TTTGTTGGAG 
CGCCTTTCTT CAGGGCCAGA AGATGTCAAG ATGATCATGT TGACCACTCT GGCAGAAATG 
GAGTTGAGTG ACCACAATAA ATTGACTTTG TTTGAGAATG GAGTACTGAA GCCACTTCTT 
CACTTGGTCA CAAATGGCAA CGAGGAGATG AAGAGAGTTT CTGTTAAAGC TATTCAGAAT 
CTTTCCACTT TACCAAGGAA TGCCTTGCAG ATGATTCAGG AAGGTGCAGT GGGTCCACTA 
CTTGACCTAC TCTACCGTCA TAATGCATCC ATGATTTTGC GAGAACAAGT AGCATCAACC 
ATCATGAACC TTGCGATATC AACAGCGAAA CAAGAAGCCA AACAGACAGA AGTGACACTA 
CTTGAATATG ATGAAGACAT TTTCAAACTC TTCTCCTTGG TCAACTTGAC AGCACCTACT 
ATACAACAGA AGATACTGAG GACCTTTCAT GCTATGTGCC AACACCCATC TACAATAGAC 
ATAAGGTCTA AACTTAGACA GTGCTCGGCC ATTCAAATGT TGGTTCAATT TTGTGAGGTT 
GATGATATTG CTCTACGAGC AAATGCTTTA AAGCTGTTCT TCTGCTTGAC GGAAGATGGA 
GATGATGGCT CGTTGTCAGA ATATGTGGAC CAGAGGTGCA TTGGGACCTT GATCAGGATC 
ATCAGAGATT GTCATGATGA GGAAGAAATT ACTGCTGCTC TGGGTATTAT CTCCAACCTT 
CCAATGAAGA ACATCCAAAT CACCCAGTGG CTTCTAGATG CTGAGGCACT TCCAGTAATT 
TTCAGGTTCC TAACTAATGG AAAATTTAAT GGATCCTATA AAAACGATCT AATAGAGAAT 
GCAGTTGGAG CAACATGCCG CTTCACTGTA TCAACAAATC TGGAGTGGCA GAAGAGAGCA 
GCTGAAGCTG GCATTATCCC AGTGCTAGTA CAACTGCTAA GCTCTGGAAC TGCTTTGACA 
AAACAACATG CGGCCACTTC CCTTGCCCAG TTCTCAGAGA GTTCAGTTAG GTTGAGTAGG 
CCAATCAAGA GACATAGGGG GCTCTGGTGC TGCTCACCAC CACCTGAAGT TGGCTGCCCA 
GTGCACATGG GCATATGTAC AGTTGAGTTA TCATTTTGCC TTGTCGAGGC TGGAGCTGTG 
CAACCCCTAG TAACTATACT TGGAGAATCA GATTTTGGAG CCTCCAAGGC TTCTTTACAG 
GCATTGTTGA CACTGATTGA TGGTGAAAGA CTGCAGAGTG GTAGTAAGGT GCTTGCTGAA 
GCAAATGCCA TTCCACCAAT TATTAAGTTA TTAGGCTCCC CTTCTACTGA ATTGCAGGAA 
AGGGCTCTTC TTGCTTTGGA AAGAATATTC CGGTTGATAG AATTCAAACA AAAGTACGGA 
GCATCAGCTC AGATGCCTTT AGTTGACTTA ACTCAGAGGG GAAACAGTAC TACAAAACCT 
TTGGCAGCAC GGATTCTTGC ACACTTGAAT GTACTTCACG AGTCATCATC TTATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010252534.1
CDS	478..3537
Translation	MLVTSLFFLLKEMATNTMINVSLVPASELLDRIIETIAETAEAAKDVLVEKEGFAKLSSFLEKVVPILKELTKKNISNSESLNNAIENFDREVKAAKQLVLECRKRNKVYLFLNCRRVVKRLEQTTREISRALSFIPLASLDLSSSINEDISNLCDNMQRAEFKAAVAEEEILEKIELGILERNVDRTYANNLMVLIAEAVGISTERSALKKEFEDFKREIEDTQVRKDQAEAIQMDQIVALLERADATSSPREKEMKYFTKRKSLGNQPLEPLQSFYCPITRDVMVDPVETSSGQTFERDAIQKWFADGNTICPLTMIPLDTAILRPNKTLRQSIEEWRDRNIMITIASMKPKLHSDDEQEILHSLEQLQDLCMERDLHREWVTLENYIPILIGLLSTKNREIRNHVLDILCILAKDSDDTKERIAEVENAIEAIVRSLARRISESKLAVALLLELSKSNMVRNYIGKVQGCILLLVTMSTSDDTQAAKDAKELLENLSFLDENVVQMAKANYFRPLLERLSSGPEDVKMIMLTTLAEMELSDHNKLTLFENGVLKPLLHLVTNGNEEMKRVSVKAIQNLSTLPRNALQMIQEGAVGPLLDLLYRHNASMILREQVASTIMNLAISTAKQEAKQTEVTLLEYDEDIFKLFSLVNLTAPTIQQKILRTFHAMCQHPSTIDIRSKLRQCSAIQMLVQFCEVDDIALRANALKLFFCLTEDGDDGSLSEYVDQRCIGTLIRIIRDCHDEEEITAALGIISNLPMKNIQITQWLLDAEALPVIFRFLTNGKFNGSYKNDLIENAVGATCRFTVSTNLEWQKRAAEAGIIPVLVQLLSSGTALTKQHAATSLAQFSESSVRLSRPIKRHRGLWCCSPPPEVGCPVHMGICTVELSFCLVEAGAVQPLVTILGESDFGASKASLQALLTLIDGERLQSGSKVLAEANAIPPIIKLLGSPSTELQERALLALERIFRLIEFKQKYGASAQMPLVDLTQRGNSTTKPLAARILAHLNVLHESSSYF

Target ORF information:

RefSeq Version	XM_010254232.2
Organism	Nelumbo nucifera(sacred lotus)
Definition	Nelumbo nucifera U-box domain-containing protein 44-like (LOC104594089), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010254232.2

ORF Insert Sequence:

ATGCTGGTTA CTAGCTTATT TTTCCTGCTA AAAGAGATGG CAACAAATAC CATGATTAAT 
GTTTCACTTG TTCCGGCTTC AGAATTACTT GATCGGATAA TAGAGACGAT AGCCGAGACT 
GCAGAGGCTG CTAAGGATGT GCTTGTTGAG AAGGAAGGTT TTGCAAAGCT GTCGAGTTTC 
TTGGAAAAGG TTGTTCCTAT CCTAAAAGAA TTGACCAAGA AGAACATCAG TAACTCTGAG 
AGCCTAAATA ATGCCATTGA AAATTTCGAT CGGGAGGTCA AAGCTGCGAA GCAGCTAGTT 
CTGGAGTGCA GAAAGAGAAA CAAAGTCTAT CTCTTTCTCA ATTGCCGGAG AGTAGTGAAG 
CGTTTAGAGC AAACTACGAG AGAGATCAGC CGGGCTCTGA GCTTTATCCC TTTGGCATCA 
CTAGATCTCT CATCTAGTAT AAATGAGGAT ATCAGCAACC TATGTGACAA CATGCAGAGG 
GCAGAGTTTA AGGCAGCTGT AGCAGAGGAA GAGATATTGG AGAAAATTGA ATTGGGTATA 
TTAGAGAGAA ATGTTGATCG TACTTACGCA AACAATTTGA TGGTTCTCAT TGCTGAAGCT 
GTTGGGATAT CAACTGAACG CTCAGCACTG AAGAAGGAAT TTGAAGATTT CAAAAGAGAA 
ATAGAAGACA CGCAGGTGAG GAAAGACCAG GCCGAGGCTA TACAAATGGA TCAGATCGTT 
GCATTGCTTG AAAGAGCTGA TGCCACTTCA TCCCCTAGGG AAAAAGAGAT GAAATACTTC 
ACCAAGAGGA AGTCTTTAGG TAATCAGCCA TTGGAACCTC TCCAGTCATT TTATTGCCCA 
ATCACTCGAG ATGTTATGGT GGACCCTGTT GAGACCTCCT CTGGACAGAC ATTCGAGAGA 
GATGCAATTC AGAAGTGGTT TGCAGATGGG AACACCATTT GCCCCCTAAC CATGATCCCC 
TTAGACACAG CAATTTTACG GCCCAACAAA ACTCTACGGC AATCAATTGA AGAATGGAGG 
GATAGAAACA TCATGATTAC AATTGCTTCC ATGAAACCCA AACTCCATTC AGATGATGAA 
CAAGAAATTC TTCATTCCTT GGAACAACTG CAGGATCTTT GCATGGAGAG AGACTTGCAC 
CGAGAATGGG TGACACTGGA GAACTACATA CCAATTCTTA TTGGATTGTT ATCCACAAAA 
AATCGTGAGA TAAGGAACCA TGTTTTAGAC ATCCTTTGCA TACTGGCAAA GGATAGTGAT 
GACACTAAGG AAAGAATTGC TGAAGTGGAA AATGCAATTG AAGCTATTGT GCGATCCCTT 
GCTCGTCGTA TTAGTGAAAG TAAGCTTGCT GTGGCATTGC TGTTGGAACT TTCAAAAAGT 
AATATGGTAC GTAACTACAT TGGGAAAGTA CAGGGATGTA TACTCCTCCT GGTGACTATG 
TCAACGAGTG ATGACACTCA AGCTGCCAAA GATGCAAAGG AACTCTTGGA GAATCTTTCA 
TTCCTTGATG AAAATGTTGT ACAAATGGCC AAGGCAAATT ATTTCAGGCC TTTGTTGGAG 
CGCCTTTCTT CAGGGCCAGA AGATGTCAAG ATGATCATGT TGACCACTCT GGCAGAAATG 
GAGTTGAGTG ACCACAATAA ATTGACTTTG TTTGAGAATG GAGTACTGAA GCCACTTCTT 
CACTTGGTCA CAAATGGCAA CGAGGAGATG AAGAGAGTTT CTGTTAAAGC TATTCAGAAT 
CTTTCCACTT TACCAAGGAA TGCCTTGCAG ATGATTCAGG AAGGTGCAGT GGGTCCACTA 
CTTGACCTAC TCTACCGTCA TAATGCATCC ATGATTTTGC GAGAACAAGT AGCATCAACC 
ATCATGAACC TTGCGATATC AACAGCGAAA CAAGAAGCCA AACAGACAGA AGTGACACTA 
CTTGAATATG ATGAAGACAT TTTCAAACTC TTCTCCTTGG TCAACTTGAC AGCACCTACT 
ATACAACAGA AGATACTGAG GACCTTTCAT GCTATGTGCC AACACCCATC TACAATAGAC 
ATAAGGTCTA AACTTAGACA GTGCTCGGCC ATTCAAATGT TGGTTCAATT TTGTGAGGTT 
GATGATATTG CTCTACGAGC AAATGCTTTA AAGCTGTTCT TCTGCTTGAC GGAAGATGGA 
GATGATGGCT CGTTGTCAGA ATATGTGGAC CAGAGGTGCA TTGGGACCTT GATCAGGATC 
ATCAGAGATT GTCATGATGA GGAAGAAATT ACTGCTGCTC TGGGTATTAT CTCCAACCTT 
CCAATGAAGA ACATCCAAAT CACCCAGTGG CTTCTAGATG CTGAGGCACT TCCAGTAATT 
TTCAGGTTCC TAACTAATGG AAAATTTAAT GGATCCTATA AAAACGATCT AATAGAGAAT 
GCAGTTGGAG CAACATGCCG CTTCACTGTA TCAACAAATC TGGAGTGGCA GAAGAGAGCA 
GCTGAAGCTG GCATTATCCC AGTGCTAGTA CAACTGCTAA GCTCTGGAAC TGCTTTGACA 
AAACAACATG CGGCCACTTC CCTTGCCCAG TTCTCAGAGA GTTCAGTTAG GTTGAGTAGG 
CCAATCAAGA GACATAGGGG GCTCTGGTGC TGCTCACCAC CACCTGAAGT TGGCTGCCCA 
GTGCACATGG GCATATGTAC AGTTGAGTTA TCATTTTGCC TTGTCGAGGC TGGAGCTGTG 
CAACCCCTAG TAACTATACT TGGAGAATCA GATTTTGGAG CCTCCAAGGC TTCTTTACAG 
GCATTGTTGA CACTGATTGA TGGTGAAAGA CTGCAGAGTG GTAGTAAGGT GCTTGCTGAA 
GCAAATGCCA TTCCACCAAT TATTAAGTTA TTAGGCTCCC CTTCTACTGA ATTGCAGGAA 
AGGGCTCTTC TTGCTTTGGA AAGAATATTC CGGTTGATAG AATTCAAACA AAAGTACGGA 
GCATCAGCTC AGATGCCTTT AGTTGACTTA ACTCAGAGGG GAAACAGTAC TACAAAACCT 
TTGGCAGCAC GGATTCTTGC ACACTTGAAT GTACTTCACG AGTCATCATC TTATTTTTAG