MEPCE cDNA ORF clone, Nanorana parkeri

Gene
Clones
gRNAs

Summary

Gene Symbol	MEPCE
Entrez Gene ID	108802527
Full Name	methylphosphate capping enzyme
Gene Type	protein-coding
Organism	Nanorana parkeri

mRNA and Protein(s)

mRNA	Protein	Name
XM_018574388.1	XP_018429890.1	LOW QUALITY PROTEIN: 7SK snRNA methylphosphate capping enzyme

Pathways from BioSystems
Gene Ontology

The following MEPCE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEPCE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONa69443	XM_018574388.1 Latest version!	Nanorana parkeri methylphosphate capping enzyme (MEPCE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MEPCE ( XM_018574388.1 ) cDNA ORF clone, Nanorana parkeri -> XP_018429890.1 Nanorana parkeri methylphosphate capping enzyme (MEPCE), mRNA.

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CloneID	ONa69443
Clone ID Related Accession (Same CDS sequence)	XM_018574388.1
Accession Version	XM_018574388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-29
Organism	Nanorana parkeri
Product	LOW QUALITY PROTEIN: 7SK snRNA methylphosphate capping enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017309185.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nanorana parkeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGCAGGTCC TGTTAGAAAT GGCGTCTTCC CCGGAGGCCT TGCAGCAGCC TGTCGAAACT 
GCCCCTAAAG ATGGGGAAAC TACTACCATG GCTCCGCCCG CTGTCCCTCC CCCGCAGCCA 
GACGTCAAAC TCGCGAAGGA GGGCGCGGAG CCGCAGCTGC CGCCTCGCGT GCCGAAGAAC 
GGCTTCCAGG GGAAGAGGAG GAACAGCTTC AACGTGGGCT TCAAGCACCC GCTGCCATTC 
AAGCGGCGGA GGCGGGTCAA CTCGGACTGC GATCCCGTCC TGCCTTCCAA CTTCCACCTC 
GGGGGCAACA TCTTTGACCC GCTCAACCTC AACAGCTTGC TAGACGAGGA TGTCAACCGG 
ACCATCAACG CCGAGACCCC AAAGTCTTCG CCGCTTCCTC CTCGCAACCG CGACCCCGTG 
GAGATTTTGA TCCCCAAGGA CATTACCGAC CCACTGAGCC TTAACACCGA TGCCAGCGAC 
GTGGAGCTCC TCCTCTCCCC GCTCAAGAGC GGTAGAAAAA GGCGCCGCCA TCGGCATCAC 
CCGTCCACGG ACGTACCCAC CAAGGGGACG GTGGGGCTGG TCGAAGAGGT CGAACCGACG 
CCTGAGGAGC TACGGCCATA CGAACTCAAC ACTGCCATTA ATTGCCGCGA TGACGTCGTC 
CCTTTGCCCT CCCTGGACAA CGAAGGACCC GATGGGGCGA CGGCCAGCGG CACGTGTACG 
TCAGGCACGA GGCACCGCAA GAGGAGGAGG ACATCCAGCA AGTCGGAAGG GAGGCAGGGG 
AGTCCCGAGA AAGGCAAGAC TTTGGCCAGT GTGGTCACCA CCGCCAAGCC TCAAGGCAGT 
ATGGTGGTGG CCTCCAAGCT GCCCTGTTCT GGGCCCAAGG GCAAAGAGAA GAGCCGCTTC 
CAGTACGGGA ATTACTGCCG GTACTACGGA TACCGGAACC CTTGCCGGAC GGACGATCCC 
CGCATGCGGG CTCTGAGGCC CGAGTGGTTC GGGGGCAAGA CGGTGTTGGA CATTGGCTGC 
AACGTTGGCC ATCTGACCCT ATGTGTGGCA CAGCATCTGA GACCCTCGCT CATAGTGGGT 
CTGGACATTG ACGGGTCGTT GATTCGAGCC GCCCGGCAGA ACATAAGACA TTATCTCTCG 
GAGTACGAGA AAGGCGGTGG TGGCAGCTAC CCCGCGGCAC TGGCTGCCAT GCGGGGCCCC 
ATCGCTGCTC CGGTTCTGCC AACTGAGGCA GGGAAGAGGA GCAAAGACGA GAACTGCGTC 
TTCCCGCACA ACGTGACGTT CGTCAAGGGT AACTACGTGG TGGAGCGGGA TGAGCTGGTG 
GAGGTGCAGC AGCCGGAATA CGACGTCGTC TTGTGTCTCA GCATCAGCAA GTGGGTCCAT 
CTCAACTGGG GAGACCAGGG GCTGAAACGC ATGTTCCGGA GAATCTACAG GCACCTGTCT 
CCGGGGGGGG TCTTCATCCT GGAGCCCCAA CCCTGGTCCT CGTACGGCAA GAGGAAGAAG 
CTGACGGAGG GGCTATAATG GGGACCGGCT TGTGATTTTA TCTCCCTGAA GCCGGACCAG 
TTCACCTCCT ACCTCATGTC TTCCGAAGTC GGCTTCTCCA GTTATGAACT GCTGGCTGCG 
CCGTGCGCCT CAACGAAAGG GTTCCAGCGT CCGATCTACG CGTTTCACAA ATCGGGCCCC 
CGAGTGGAGC TTCCTACGCC GTCCTCGCCC CGCAGCGCGC CCCCGGTCCA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018429890.1
CDS	303..2039
Translation	MQVLLEMASSPEALQQPVETAPKDGETTTMAPPAVPPPQPDVKLAKEGAEPQLPPRVPKNGFQGKRRNSFNVGFKHPLPFKRRRRVNSDCDPVLPSNFHLGGNIFDPLNLNSLLDEDVNRTINAETPKSSPLPPRNRDPVEILIPKDITDPLSLNTDASDVELLLSPLKSGRKRRRHRHHPSTDVPTKGTVGLVEEVEPTPEELRPYELNTAINCRDDVVPLPSLDNEGPDGATASGTCTSGTRHRKRRRTSSKSEGRQGSPEKGKTLASVVTTAKPQGSMVVASKLPCSGPKGKEKSRFQYGNYCRYYGYRNPCRTDDPRMRALRPEWFGGKTVLDIGCNVGHLTLCVAQHLRPSLIVGLDIDGSLIRAARQNIRHYLSEYEKGGGGSYPAALAAMRGPIAAPVLPTEAGKRSKDENCVFPHNVTFVKGNYVVERDELVEVQQPEYDVVLCLSISKWVHLNWGDQGLKRMFRRIYRHLSPGGVFILEPQPWSSYGKRKKLTEGL*WGPACDFISLKPDQFTSYLMSSEVGFSSYELLAAPCASTKGFQRPIYAFHKSGPRVELPTPSSPRSAPPVQA

Target ORF information:

RefSeq Version	XM_018574388.1
Organism	Nanorana parkeri
Definition	Nanorana parkeri methylphosphate capping enzyme (MEPCE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018574388.1

ORF Insert Sequence:

ATGCAGGTCC TGTTAGAAAT GGCGTCTTCC CCGGAGGCCT TGCAGCAGCC TGTCGAAACT 
GCCCCTAAAG ATGGGGAAAC TACTACCATG GCTCCGCCCG CTGTCCCTCC CCCGCAGCCA 
GACGTCAAAC TCGCGAAGGA GGGCGCGGAG CCGCAGCTGC CGCCTCGCGT GCCGAAGAAC 
GGCTTCCAGG GGAAGAGGAG GAACAGCTTC AACGTGGGCT TCAAGCACCC GCTGCCATTC 
AAGCGGCGGA GGCGGGTCAA CTCGGACTGC GATCCCGTCC TGCCTTCCAA CTTCCACCTC 
GGGGGCAACA TCTTTGACCC GCTCAACCTC AACAGCTTGC TAGACGAGGA TGTCAACCGG 
ACCATCAACG CCGAGACCCC AAAGTCTTCG CCGCTTCCTC CTCGCAACCG CGACCCCGTG 
GAGATTTTGA TCCCCAAGGA CATTACCGAC CCACTGAGCC TTAACACCGA TGCCAGCGAC 
GTGGAGCTCC TCCTCTCCCC GCTCAAGAGC GGTAGAAAAA GGCGCCGCCA TCGGCATCAC 
CCGTCCACGG ACGTACCCAC CAAGGGGACG GTGGGGCTGG TCGAAGAGGT CGAACCGACG 
CCTGAGGAGC TACGGCCATA CGAACTCAAC ACTGCCATTA ATTGCCGCGA TGACGTCGTC 
CCTTTGCCCT CCCTGGACAA CGAAGGACCC GATGGGGCGA CGGCCAGCGG CACGTGTACG 
TCAGGCACGA GGCACCGCAA GAGGAGGAGG ACATCCAGCA AGTCGGAAGG GAGGCAGGGG 
AGTCCCGAGA AAGGCAAGAC TTTGGCCAGT GTGGTCACCA CCGCCAAGCC TCAAGGCAGT 
ATGGTGGTGG CCTCCAAGCT GCCCTGTTCT GGGCCCAAGG GCAAAGAGAA GAGCCGCTTC 
CAGTACGGGA ATTACTGCCG GTACTACGGA TACCGGAACC CTTGCCGGAC GGACGATCCC 
CGCATGCGGG CTCTGAGGCC CGAGTGGTTC GGGGGCAAGA CGGTGTTGGA CATTGGCTGC 
AACGTTGGCC ATCTGACCCT ATGTGTGGCA CAGCATCTGA GACCCTCGCT CATAGTGGGT 
CTGGACATTG ACGGGTCGTT GATTCGAGCC GCCCGGCAGA ACATAAGACA TTATCTCTCG 
GAGTACGAGA AAGGCGGTGG TGGCAGCTAC CCCGCGGCAC TGGCTGCCAT GCGGGGCCCC 
ATCGCTGCTC CGGTTCTGCC AACTGAGGCA GGGAAGAGGA GCAAAGACGA GAACTGCGTC 
TTCCCGCACA ACGTGACGTT CGTCAAGGGT AACTACGTGG TGGAGCGGGA TGAGCTGGTG 
GAGGTGCAGC AGCCGGAATA CGACGTCGTC TTGTGTCTCA GCATCAGCAA GTGGGTCCAT 
CTCAACTGGG GAGACCAGGG GCTGAAACGC ATGTTCCGGA GAATCTACAG GCACCTGTCT 
CCGGGGGGGG TCTTCATCCT GGAGCCCCAA CCCTGGTCCT CGTACGGCAA GAGGAAGAAG 
CTGACGGAGG GGCTATAATG GGGACCGGCT TGTGATTTTA TCTCCCTGAA GCCGGACCAG 
TTCACCTCCT ACCTCATGTC TTCCGAAGTC GGCTTCTCCA GTTATGAACT GCTGGCTGCG 
CCGTGCGCCT CAACGAAAGG GTTCCAGCGT CCGATCTACG CGTTTCACAA ATCGGGCCCC 
CGAGTGGAGC TTCCTACGCC GTCCTCGCCC CGCAGCGCGC CCCCGGTCCA GGCCTAG