Dennd4b cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dennd4b
Entrez Gene ID	103751724
Full Name	DENN domain containing 4B
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029557494.1	XP_029413354.1	DENN domain-containing protein 4B isoform X3
XM_008855239.2	XP_008853461.2	DENN domain-containing protein 4B isoform X1
XM_008855255.2	XP_008853477.1	DENN domain-containing protein 4B isoform X3
XM_029557487.1	XP_029413347.1	DENN domain-containing protein 4B isoform X1
XM_017795122.2	XP_017650611.1	DENN domain-containing protein 4B isoform X2
XM_008855255.3	XP_008853477.1	DENN domain-containing protein 4B isoform X4
XM_017795122.1	XP_017650611.1	DENN domain-containing protein 4B isoform X2

Pathways from BioSystems
Gene Ontology

The following Dennd4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dennd4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa139792	XM_029557494.1 Latest version!	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ONa139791	XM_029557487.1 Latest version!	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ONa43209	XM_008855239.2 Latest version!	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ONa43210	XM_017795122.2 Latest version!	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ONa43211	XM_008855255.3 Latest version!	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
ONa43211	XM_008855255.2	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa43210	XM_017795122.1	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dennd4b ( XM_029557494.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029413354.1 Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ONa139792
Clone ID Related Accession (Same CDS sequence)	XM_029557494.1
Accession Version	XM_029557494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	DENN domain-containing protein 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008330926.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCCGTGAG TGAAGGGGGG GCTATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TTGTGGTAGC TGGGCTCGCT GGAAACGGAG CTCCTATACC TGAGGAAACA 
TGGGTTCCTG AACCCACTGG GCCCCTGCGC CCTCCCAGGC CAGCAGAGCC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAAGTGCCCC AGGGTTACAC GTGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGTAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GTGTGTACCC 
CTACCCATGC ACCAGGGAAT ATTGGGAGGG ACCTTTAGGG TGTTGTATGA GGGGAAGGAA 
CGACCCAAGC TTGGTTTTCA AGTGCTCGAC ACGACACCAT ATAGCCACTC GGCCAACCTG 
GCCCCTCCAG GCCCTGGACA CCCCCGCACC TACCTCATGT ACCGGCGGGC AGCAGAGGGG 
GCAGGGCTGC ATGCCCTGGG CATCACTGAC CTCTGCCTGG TACTGCCCAG CAAGGGTGAA 
GGCACTCCTC ATACTTACTG CCGGTTGCCC CGCAATCTTA ACCCTGGCAT GTGGGGCCCA 
GCAGTATACC TGTGCTACAA GGTGGGCCTG GCTAAGGCCA ACACACTGGT GTATGAGGCA 
GAGCTGCTGG GCCGCTACCC AGAGGAGGAC AATGAGGCAT TCCCGCTGCC CGAGTCAGTG 
CCCGTCTTCT GCCTGCCCAT GGGGGCCACT ATCGAGTGCT GGCCTGCCCA GACCAAGTAC 
CCGGTGCCTG TCTTCTCCAC CTTTGTGCTC ACGGGTGCTG CTGGTGATAA GGTGTATGGT 
GCCGCCCTGC AGTTCTACGA AGCGTTCCCT AGGGCCAGGC TGTCAGAGAG GCAGGCACGG 
GCACTGGGCC TGCTGAGTGC TGTGGAACGG GGCCGGGCAC TGGGGGGCCG AGCTGTGCGC 
AGCCGGCGAG CCATTGCTGT ACTGTCCCGC TGGCCTGCCT TCCCAGCCTT CCGAGCCTTC 
CTTACCTTCC TTTACCGCTA CTCGGTCTCA GGCCCTCATC GCCTGCCCTT GGAAGCGCAC 
ATCTCACACT TCATTCACAA CGTCCCATTC CCTTCTCCAC AGAGACCACG CATCCTAGTG 
CAGATGTCTC CCTATGACAA CCTGCTTCTC TGTCAACCTG TGTCCTCACC CCTGCCCCTC 
AGTGGTGCTA GCTTTTTGCA GCTGCTGCAG AGCCTGGGCC CTGAGCTGGC TATTACATTG 
CTGCTGGCTG TGCTCACAGA GCACAAACTG CTGGTCCACT CACTGCGACC AGACCTGCTC 
ACCAGCGTCT GTGAGGCCCT TGTCTCAATG ATCTTCCCGC TGCACTGGCA GTGCCCCTAC 
ATTCCGCTGT GCCCGCTGGT GCTGGCGGAT GTGCTGAGTG CCCCAGTGCC CTTCATTGTG 
GGTATCCACT CCAGCTATTT TGATCTGCAC GACCCACCTG CTGATGTCAT CTGTGTTGAT 
CTTGACACCA ACACACTTTT CCAGACAGAA GAAAAGAAGC CCCTCTCCGC TAGGACCCTG 
CCACGCAGAC CCTACAAGCT TTTACTGGCT ACACTGACAA ATTTGTACCA GCAGCTGGAC 
CAGAGTTTCC TCAAGTCCCG GGAACGCTCA AGCCACAAAC TGTACTCTCA GCTGCTGCAC 
ACGCAGATGT TTTCACAGTT CATTGAGGAA TGCTCTTTTG GCTCTGCTCG GCATGCTGCC 
CTTGAATTCT TTGACTCTTG TGTTGACAAG GTCCACCCAG AGCAGGAAAA GCCGGAGCCA 
ACACCCTTAG TGGAGCTGGA GGAGCTGTCA GGAAGTGAGC TCACTGTCTT CATCACACCC 
CCAGAGGAGC CTCCAGTACT GGAGGGCAGT GAATCTATAC CACAGTACTG TTACGATGGG 
TTCCCAGAGC TAAGAGCTGA GCTATTTGAG TCTCCTCAAG AACAACAGGG GGCCTTGCCT 
GTGCCAGGCC CATCTCGTAG TGCCCCTAGC AGTCCTGCCC CTCGCCGTAC CAAACAGGAG 
ATGAAGGTTG CACAGCGGAT GGCACAGAAG TCAGCAGCTG TGCCTGAGCT GTGGGCCCGG 
TGCCTTCTGG GGCACTGCTA TGGACTGTGG TTCCTCTGCC TGCCTGCCTA TGTGCGTTCA 
GCACCCTCTC GGGTACGAGC ACTGCACACA GCCTACCATG TTCTGCGTGA GATGGAGAGC 
CGCAAAGTGG TACTCCCCGA TGAGGTGTGT TACCGAGTAC TGATGCAGCT CTGCTCACAC 
TATGGGCAGC CTGTGTTGTC TGTTCGAGTC ATGTTGGAGA TGCGGCGGGC TGGCATCGTG 
CCCAACACAA TCACTTATGG TTACTACAAC AAGGCTGTGC TGGAAAGCAA GTGGCCGTCT 
GGTACACCAG GTGGGCGCCT ACGCTGGGCC AAGCTCCGGA ATGTTGTCCT CGGAGCTGCT 
CAGTTCCGCC AGCCCTTGAA AGAACGTTGG CAGCAGCAGC AGCGGCAGCA GCAACAGCAG 
CGGCAGCGGC GGCGGCAGCA GCAGCAGCAA CAGATGGCCA CGCACCAAGA AAGAGGCAGC 
TCTCAGACAG AGTCCTATCT GGAGCGCCCC TCCCCAACCC GCCCTCTTCA GCGCCAGACT 
ACCTGGGCTG GCCGAAGTCT TCGGGAACCA GCCTCACCTG CTGGGCGCCT GGTCAAAAGT 
GGCAGCCTAG GCAGTGCCCG AGGGGCACAG CCTACCGTGG AGGCTGGTGT GGCTCACATG 
ATAGAAGCCT TGGGGGTCCT AGAACCCCGT GGATCACCTG TGCCTTGGCA AGATGGAAGC 
CTTTCAGACC TGAGCCTGAC AGGGGAAGAG CTGGCATCTG GGGGCAGTCC AGGGGGCTCA 
GGCTCGGCCC TGAGTGCCCA GTCCACTGAG GCCCTAGAAG GGCTAAGTGG GAGAGGTCCC 
AAAACTGGAG GATGGCAGGA GGAGGCAGGC ACCCCCAGAA GAGGACTGGG TGCCCGCCTT 
CAACAGCTGC TCACTCCTTC CCGCCGCTCC TCTGCCTCCC GCATCCCCCC ACCAGAGTTG 
TCCCCTGACC TTCCTCCTGT AGCCCGTCGC AGTCCCATGG ATAGCCTTCT GTGGCCCCGA 
GAACGCCCTG GATCCACTGC TTCTGAGAGC TCAGCCTCAC TGGGCAGTGA GTGGGATCTC 
TCAGAGTCTT CTCTCAGCAG TCTGAGTCTC CGCCATTCCT CAGAGCGCCT CAGTGACACT 
CCTGGACCCT TTCAGCCACC TTCCCTGGAA ATTCTGCTGT CTAGCTGTTC CTTGTGTCGT 
GCCTGTGATT CACTGGTGTA TGATGAGGAA ATCATGGCTG GCTGGGCACC TGATGACTCC 
AACCTCAACA CAATTTGCCC CTTCTGCGCC TGCCCGTTTG TGCCGCTCCT CAGTGTCCAG 
ACCCTTGATT CCCGACCTAG TGCCCCTAGC CCTAAGTCTA CCCTTGCCAG TGCCAGTGGT 
AGCAAAGATG CTCCTGTCCC TGGGGGTCCT GGCCCTGTGC TCAGTGACCG AAGGCTCTGT 
CTTGCCTTGG ATGAACCCCA GCTCTGCAAT GGACATTTGG GGGGTGTCTC CCGGCGGGTT 
GAGAGTGGGG CATGGGCATA CCTAAGCCCC CTGGTGCTGC GTAAGGAGCT AGAGTCGCTG 
GTAGAGAATG AAGGCAGCGA GGTGTTGGCA TTGCCTGAAC TGCCTGCTGC CCACCCCATC 
ATTTTCTGGA ACCTTCTGTG GTATTTCCAA CGGCTACGCC TGCCTAGTAT TCTACCAGGC 
CTAGTGCTGG CCTCTTATGA TGGGCCCCCA CCCAACCAGC TCTCCCAGGC ACCATCTCCT 
TGGTTAACCC CTGATCCAGC CTCTGTGCAT GTGCGGCTGT TGTGGGATGT CTTGACCCCT 
GATCCTAACA GCTGCCCACC TCTCTACGTG CTCTGGAGGG TCCACAGCCA GATCCCTCAA 
CGGGTGGTAT GGCCAGGCCC AGTACCTTCA TGTCTTAGCT TGGCATTATT GGAGTCTGTG 
CTGCGTCATG TTGGACTTAA TGAGGTGCAC AAGGCTGTGG GGCTTCTGCT GGAAACTCTA 
GGACCCCCTC CCACTGGCCT GCACCTGCAG AGGAGCATCT ATCGTGAGAT CTTATTCCTG 
ACAATGGCAG CTCTGGGCAA GGATCATGTG GACATAGTGG CCTTCGACAA GAAGTACAAG 
TCTGCCTTTA ACAAGCTGGC CAGCAGCATG GGCAAGGAGG AACTAAGGCA ACGACGGGCA 
CAGATGCCTA CTCCCAAGGC GATTGACTGC CGAAAATGTT TTGGAGCACC TCTGGAATGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029413354.1
CDS	149..4531
Translation	MEADAVSEGGAMAEERPPRLVDYFVVAGLAGNGAPIPEETWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGCVPLPMHQGILGGTFRVLYEGKERPKLGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSARTLPRRPYKLLLATLTNLYQQLDQSFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESIPQYCYDGFPELRAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYHVLREMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKERWQQQQRQQQQQRQRRRQQQQQQMATHQERGSSQTESYLERPSPTRPLQRQTTWAGRSLREPASPAGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEELASGGSPGGSGSALSAQSTEALEGLSGRGPKTGGWQEEAGTPRRGLGARLQQLLTPSRRSSASRIPPPELSPDLPPVARRSPMDSLLWPRERPGSTASESSASLGSEWDLSESSLSSLSLRHSSERLSDTPGPFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTICPFCACPFVPLLSVQTLDSRPSAPSPKSTLASASGSKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHLGGVSRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPGLVLASYDGPPPNQLSQAPSPWLTPDPASVHVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRSIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_029557494.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029557494.1

ORF Insert Sequence:

ATGGAGGCAG ATGCCGTGAG TGAAGGGGGG GCTATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TTGTGGTAGC TGGGCTCGCT GGAAACGGAG CTCCTATACC TGAGGAAACA 
TGGGTTCCTG AACCCACTGG GCCCCTGCGC CCTCCCAGGC CAGCAGAGCC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAAGTGCCCC AGGGTTACAC GTGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGTAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GTGTGTACCC 
CTACCCATGC ACCAGGGAAT ATTGGGAGGG ACCTTTAGGG TGTTGTATGA GGGGAAGGAA 
CGACCCAAGC TTGGTTTTCA AGTGCTCGAC ACGACACCAT ATAGCCACTC GGCCAACCTG 
GCCCCTCCAG GCCCTGGACA CCCCCGCACC TACCTCATGT ACCGGCGGGC AGCAGAGGGG 
GCAGGGCTGC ATGCCCTGGG CATCACTGAC CTCTGCCTGG TACTGCCCAG CAAGGGTGAA 
GGCACTCCTC ATACTTACTG CCGGTTGCCC CGCAATCTTA ACCCTGGCAT GTGGGGCCCA 
GCAGTATACC TGTGCTACAA GGTGGGCCTG GCTAAGGCCA ACACACTGGT GTATGAGGCA 
GAGCTGCTGG GCCGCTACCC AGAGGAGGAC AATGAGGCAT TCCCGCTGCC CGAGTCAGTG 
CCCGTCTTCT GCCTGCCCAT GGGGGCCACT ATCGAGTGCT GGCCTGCCCA GACCAAGTAC 
CCGGTGCCTG TCTTCTCCAC CTTTGTGCTC ACGGGTGCTG CTGGTGATAA GGTGTATGGT 
GCCGCCCTGC AGTTCTACGA AGCGTTCCCT AGGGCCAGGC TGTCAGAGAG GCAGGCACGG 
GCACTGGGCC TGCTGAGTGC TGTGGAACGG GGCCGGGCAC TGGGGGGCCG AGCTGTGCGC 
AGCCGGCGAG CCATTGCTGT ACTGTCCCGC TGGCCTGCCT TCCCAGCCTT CCGAGCCTTC 
CTTACCTTCC TTTACCGCTA CTCGGTCTCA GGCCCTCATC GCCTGCCCTT GGAAGCGCAC 
ATCTCACACT TCATTCACAA CGTCCCATTC CCTTCTCCAC AGAGACCACG CATCCTAGTG 
CAGATGTCTC CCTATGACAA CCTGCTTCTC TGTCAACCTG TGTCCTCACC CCTGCCCCTC 
AGTGGTGCTA GCTTTTTGCA GCTGCTGCAG AGCCTGGGCC CTGAGCTGGC TATTACATTG 
CTGCTGGCTG TGCTCACAGA GCACAAACTG CTGGTCCACT CACTGCGACC AGACCTGCTC 
ACCAGCGTCT GTGAGGCCCT TGTCTCAATG ATCTTCCCGC TGCACTGGCA GTGCCCCTAC 
ATTCCGCTGT GCCCGCTGGT GCTGGCGGAT GTGCTGAGTG CCCCAGTGCC CTTCATTGTG 
GGTATCCACT CCAGCTATTT TGATCTGCAC GACCCACCTG CTGATGTCAT CTGTGTTGAT 
CTTGACACCA ACACACTTTT CCAGACAGAA GAAAAGAAGC CCCTCTCCGC TAGGACCCTG 
CCACGCAGAC CCTACAAGCT TTTACTGGCT ACACTGACAA ATTTGTACCA GCAGCTGGAC 
CAGAGTTTCC TCAAGTCCCG GGAACGCTCA AGCCACAAAC TGTACTCTCA GCTGCTGCAC 
ACGCAGATGT TTTCACAGTT CATTGAGGAA TGCTCTTTTG GCTCTGCTCG GCATGCTGCC 
CTTGAATTCT TTGACTCTTG TGTTGACAAG GTCCACCCAG AGCAGGAAAA GCCGGAGCCA 
ACACCCTTAG TGGAGCTGGA GGAGCTGTCA GGAAGTGAGC TCACTGTCTT CATCACACCC 
CCAGAGGAGC CTCCAGTACT GGAGGGCAGT GAATCTATAC CACAGTACTG TTACGATGGG 
TTCCCAGAGC TAAGAGCTGA GCTATTTGAG TCTCCTCAAG AACAACAGGG GGCCTTGCCT 
GTGCCAGGCC CATCTCGTAG TGCCCCTAGC AGTCCTGCCC CTCGCCGTAC CAAACAGGAG 
ATGAAGGTTG CACAGCGGAT GGCACAGAAG TCAGCAGCTG TGCCTGAGCT GTGGGCCCGG 
TGCCTTCTGG GGCACTGCTA TGGACTGTGG TTCCTCTGCC TGCCTGCCTA TGTGCGTTCA 
GCACCCTCTC GGGTACGAGC ACTGCACACA GCCTACCATG TTCTGCGTGA GATGGAGAGC 
CGCAAAGTGG TACTCCCCGA TGAGGTGTGT TACCGAGTAC TGATGCAGCT CTGCTCACAC 
TATGGGCAGC CTGTGTTGTC TGTTCGAGTC ATGTTGGAGA TGCGGCGGGC TGGCATCGTG 
CCCAACACAA TCACTTATGG TTACTACAAC AAGGCTGTGC TGGAAAGCAA GTGGCCGTCT 
GGTACACCAG GTGGGCGCCT ACGCTGGGCC AAGCTCCGGA ATGTTGTCCT CGGAGCTGCT 
CAGTTCCGCC AGCCCTTGAA AGAACGTTGG CAGCAGCAGC AGCGGCAGCA GCAACAGCAG 
CGGCAGCGGC GGCGGCAGCA GCAGCAGCAA CAGATGGCCA CGCACCAAGA AAGAGGCAGC 
TCTCAGACAG AGTCCTATCT GGAGCGCCCC TCCCCAACCC GCCCTCTTCA GCGCCAGACT 
ACCTGGGCTG GCCGAAGTCT TCGGGAACCA GCCTCACCTG CTGGGCGCCT GGTCAAAAGT 
GGCAGCCTAG GCAGTGCCCG AGGGGCACAG CCTACCGTGG AGGCTGGTGT GGCTCACATG 
ATAGAAGCCT TGGGGGTCCT AGAACCCCGT GGATCACCTG TGCCTTGGCA AGATGGAAGC 
CTTTCAGACC TGAGCCTGAC AGGGGAAGAG CTGGCATCTG GGGGCAGTCC AGGGGGCTCA 
GGCTCGGCCC TGAGTGCCCA GTCCACTGAG GCCCTAGAAG GGCTAAGTGG GAGAGGTCCC 
AAAACTGGAG GATGGCAGGA GGAGGCAGGC ACCCCCAGAA GAGGACTGGG TGCCCGCCTT 
CAACAGCTGC TCACTCCTTC CCGCCGCTCC TCTGCCTCCC GCATCCCCCC ACCAGAGTTG 
TCCCCTGACC TTCCTCCTGT AGCCCGTCGC AGTCCCATGG ATAGCCTTCT GTGGCCCCGA 
GAACGCCCTG GATCCACTGC TTCTGAGAGC TCAGCCTCAC TGGGCAGTGA GTGGGATCTC 
TCAGAGTCTT CTCTCAGCAG TCTGAGTCTC CGCCATTCCT CAGAGCGCCT CAGTGACACT 
CCTGGACCCT TTCAGCCACC TTCCCTGGAA ATTCTGCTGT CTAGCTGTTC CTTGTGTCGT 
GCCTGTGATT CACTGGTGTA TGATGAGGAA ATCATGGCTG GCTGGGCACC TGATGACTCC 
AACCTCAACA CAATTTGCCC CTTCTGCGCC TGCCCGTTTG TGCCGCTCCT CAGTGTCCAG 
ACCCTTGATT CCCGACCTAG TGCCCCTAGC CCTAAGTCTA CCCTTGCCAG TGCCAGTGGT 
AGCAAAGATG CTCCTGTCCC TGGGGGTCCT GGCCCTGTGC TCAGTGACCG AAGGCTCTGT 
CTTGCCTTGG ATGAACCCCA GCTCTGCAAT GGACATTTGG GGGGTGTCTC CCGGCGGGTT 
GAGAGTGGGG CATGGGCATA CCTAAGCCCC CTGGTGCTGC GTAAGGAGCT AGAGTCGCTG 
GTAGAGAATG AAGGCAGCGA GGTGTTGGCA TTGCCTGAAC TGCCTGCTGC CCACCCCATC 
ATTTTCTGGA ACCTTCTGTG GTATTTCCAA CGGCTACGCC TGCCTAGTAT TCTACCAGGC 
CTAGTGCTGG CCTCTTATGA TGGGCCCCCA CCCAACCAGC TCTCCCAGGC ACCATCTCCT 
TGGTTAACCC CTGATCCAGC CTCTGTGCAT GTGCGGCTGT TGTGGGATGT CTTGACCCCT 
GATCCTAACA GCTGCCCACC TCTCTACGTG CTCTGGAGGG TCCACAGCCA GATCCCTCAA 
CGGGTGGTAT GGCCAGGCCC AGTACCTTCA TGTCTTAGCT TGGCATTATT GGAGTCTGTG 
CTGCGTCATG TTGGACTTAA TGAGGTGCAC AAGGCTGTGG GGCTTCTGCT GGAAACTCTA 
GGACCCCCTC CCACTGGCCT GCACCTGCAG AGGAGCATCT ATCGTGAGAT CTTATTCCTG 
ACAATGGCAG CTCTGGGCAA GGATCATGTG GACATAGTGG CCTTCGACAA GAAGTACAAG 
TCTGCCTTTA ACAAGCTGGC CAGCAGCATG GGCAAGGAGG AACTAAGGCA ACGACGGGCA 
CAGATGCCTA CTCCCAAGGC GATTGACTGC CGAAAATGTT TTGGAGCACC TCTGGAATGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_029557487.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029413347.1 Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONa139791
Clone ID Related Accession (Same CDS sequence)	XM_029557487.1
Accession Version	XM_029557487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	DENN domain-containing protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008330926.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCCGTGAG TGAAGGGGGG GCTATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TTGTGGTAGC TGGGCTCGCT GGAAACGGAG CTCCTATACC TGAGGAAACA 
TGGGTTCCTG AACCCACTGG GCCCCTGCGC CCTCCCAGGC CAGCAGAGCC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAAGTGCCCC AGGGTTACAC GTGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGTAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GTGTGTACCC 
CTACCCATGC ACCAGGGAAT ATTGGGAGGG ACCTTTAGGG TGTTGTATGA GGGGAAGGAA 
CGACCCAAGC TTGGTTTTCA AGTGCTCGAC ACGACACCAT ATAGCCACTC GGCCAACCTG 
GCCCCTCCAG GCCCTGGACA CCCCCGCACC TACCTCATGT ACCGGCGGGC AGCAGAGGGG 
GCAGGGCTGC ATGCCCTGGG CATCACTGAC CTCTGCCTGG TACTGCCCAG CAAGGGTGAA 
GGCACTCCTC ATACTTACTG CCGGTTGCCC CGCAATCTTA ACCCTGGCAT GTGGGGCCCA 
GCAGTATACC TGTGCTACAA GGTGGGCCTG GCTAAGGCCA ACACACTGGT GTATGAGGCA 
GAGCTGCTGG GCCGCTACCC AGAGGAGGAC AATGAGGCAT TCCCGCTGCC CGAGTCAGTG 
CCCGTCTTCT GCCTGCCCAT GGGGGCCACT ATCGAGTGCT GGCCTGCCCA GACCAAGTAC 
CCGGTGCCTG TCTTCTCCAC CTTTGTGCTC ACGGGTGCTG CTGGTGATAA GGTGTATGGT 
GCCGCCCTGC AGTTCTACGA AGCGTTCCCT AGGGCCAGGC TGTCAGAGAG GCAGGCACGG 
GCACTGGGCC TGCTGAGTGC TGTGGAACGG GGCCGGGCAC TGGGGGGCCG AGCTGTGCGC 
AGCCGGCGAG CCATTGCTGT ACTGTCCCGC TGGCCTGCCT TCCCAGCCTT CCGAGCCTTC 
CTTACCTTCC TTTACCGCTA CTCGGTCTCA GGCCCTCATC GCCTGCCCTT GGAAGCGCAC 
ATCTCACACT TCATTCACAA CGTCCCATTC CCTTCTCCAC AGAGACCACG CATCCTAGTG 
CAGATGTCTC CCTATGACAA CCTGCTTCTC TGTCAACCTG TGTCCTCACC CCTGCCCCTC 
AGTGGTGCTA GCTTTTTGCA GCTGCTGCAG AGCCTGGGCC CTGAGCTGGC TATTACATTG 
CTGCTGGCTG TGCTCACAGA GCACAAACTG CTGGTCCACT CACTGCGACC AGACCTGCTC 
ACCAGCGTCT GTGAGGCCCT TGTCTCAATG ATCTTCCCGC TGCACTGGCA GTGCCCCTAC 
ATTCCGCTGT GCCCGCTGGT GCTGGCGGAT GTGCTGAGTG CCCCAGTGCC CTTCATTGTG 
GGTATCCACT CCAGCTATTT TGATCTGCAC GACCCACCTG CTGATGTCAT CTGTGTTGAT 
CTTGACACCA ACACACTTTT CCAGACAGAA GAAAAGAAGC CCCTCTCCGC TAGGACCCTG 
CCACGCAGAC CCTACAAGCT TTTACTGGCT ACACTGACAA ATTTGTACCA GCAGCTGGAC 
CAGACTTACA CTGGACCAGA GGAGGAGGCC TCCCTGGAAT TCCTGCTGAC AGACTACGAG 
GCAGTGTGTG GCCGTAGGGC ACGCCTGGAG CGGGAAGTCC AGGGAGCCTT CCTGCGCTTC 
ATGGCCTGTC TGCTCAAAGG CTACCGGGAC TTTCTGCGCC CCCTCACCCA GGCCCCCTCT 
GAGGGCTCTC GTGATGTTGA CAACCTTTTC TACCTTCAGG GTTTCCTCAA GTCCCGGGAA 
CGCTCAAGCC ACAAACTGTA CTCTCAGCTG CTGCACACGC AGATGTTTTC ACAGTTCATT 
GAGGAATGCT CTTTTGGCTC TGCTCGGCAT GCTGCCCTTG AATTCTTTGA CTCTTGTGTT 
GACAAGGTCC ACCCAGAGCA GGAAAAGCCG GAGCCAACAC CCTTAGTGGA GCTGGAGGAG 
CTGTCAGGAA GTGAGCTCAC TGTCTTCATC ACACCCCCAG AGGAGCCTCC AGTACTGGAG 
GGCAGTGAAT CTATACCACA GTACTGTTAC GATGGGTTCC CAGAGCTAAG AGCTGAGCTA 
TTTGAGTCTC CTCAAGAACA ACAGGGGGCC TTGCCTGTGC CAGGCCCATC TCGTAGTGCC 
CCTAGCAGTC CTGCCCCTCG CCGTACCAAA CAGGAGATGA AGGTTGCACA GCGGATGGCA 
CAGAAGTCAG CAGCTGTGCC TGAGCTGTGG GCCCGGTGCC TTCTGGGGCA CTGCTATGGA 
CTGTGGTTCC TCTGCCTGCC TGCCTATGTG CGTTCAGCAC CCTCTCGGGT ACGAGCACTG 
CACACAGCCT ACCATGTTCT GCGTGAGATG GAGAGCCGCA AAGTGGTACT CCCCGATGAG 
GTGTGTTACC GAGTACTGAT GCAGCTCTGC TCACACTATG GGCAGCCTGT GTTGTCTGTT 
CGAGTCATGT TGGAGATGCG GCGGGCTGGC ATCGTGCCCA ACACAATCAC TTATGGTTAC 
TACAACAAGG CTGTGCTGGA AAGCAAGTGG CCGTCTGGTA CACCAGGTGG GCGCCTACGC 
TGGGCCAAGC TCCGGAATGT TGTCCTCGGA GCTGCTCAGT TCCGCCAGCC CTTGAAAGAA 
CGTTGGCAGC AGCAGCAGCG GCAGCAGCAA CAGCAGCGGC AGCGGCGGCG GCAGCAGCAG 
CAGCAACAGA TGGCCACGCA CCAAGAAAGA GGCAGCTCTC AGACAGAGTC CTATCTGGAG 
CGCCCCTCCC CAACCCGCCC TCTTCAGCGC CAGACTACCT GGGCTGGCCG AAGTCTTCGG 
GAACCAGCCT CACCTGCTGG GCGCCTGGTC AAAAGTGGCA GCCTAGGCAG TGCCCGAGGG 
GCACAGCCTA CCGTGGAGGC TGGTGTGGCT CACATGATAG AAGCCTTGGG GGTCCTAGAA 
CCCCGTGGAT CACCTGTGCC TTGGCAAGAT GGAAGCCTTT CAGACCTGAG CCTGACAGGG 
GAAGAGCTGG CATCTGGGGG CAGTCCAGGG GGCTCAGGCT CGGCCCTGAG TGCCCAGTCC 
ACTGAGGCCC TAGAAGGGCT AAGTGGGAGA GGTCCCAAAA CTGGAGGATG GCAGGAGGAG 
GCAGGCACCC CCAGAAGAGG ACTGGGTGCC CGCCTTCAAC AGCTGCTCAC TCCTTCCCGC 
CGCTCCTCTG CCTCCCGCAT CCCCCCACCA GAGTTGTCCC CTGACCTTCC TCCTGTAGCC 
CGTCGCAGTC CCATGGATAG CCTTCTGTGG CCCCGAGAAC GCCCTGGATC CACTGCTTCT 
GAGAGCTCAG CCTCACTGGG CAGTGAGTGG GATCTCTCAG AGTCTTCTCT CAGCAGTCTG 
AGTCTCCGCC ATTCCTCAGA GCGCCTCAGT GACACTCCTG GACCCTTTCA GCCACCTTCC 
CTGGAAATTC TGCTGTCTAG CTGTTCCTTG TGTCGTGCCT GTGATTCACT GGTGTATGAT 
GAGGAAATCA TGGCTGGCTG GGCACCTGAT GACTCCAACC TCAACACAAT TTGCCCCTTC 
TGCGCCTGCC CGTTTGTGCC GCTCCTCAGT GTCCAGACCC TTGATTCCCG ACCTAGTGCC 
CCTAGCCCTA AGTCTACCCT TGCCAGTGCC AGTGGTAGCA AAGATGCTCC TGTCCCTGGG 
GGTCCTGGCC CTGTGCTCAG TGACCGAAGG CTCTGTCTTG CCTTGGATGA ACCCCAGCTC 
TGCAATGGAC ATTTGGGGGG TGTCTCCCGG CGGGTTGAGA GTGGGGCATG GGCATACCTA 
AGCCCCCTGG TGCTGCGTAA GGAGCTAGAG TCGCTGGTAG AGAATGAAGG CAGCGAGGTG 
TTGGCATTGC CTGAACTGCC TGCTGCCCAC CCCATCATTT TCTGGAACCT TCTGTGGTAT 
TTCCAACGGC TACGCCTGCC TAGTATTCTA CCAGGCCTAG TGCTGGCCTC TTATGATGGG 
CCCCCACCCA ACCAGCTCTC CCAGGCACCA TCTCCTTGGT TAACCCCTGA TCCAGCCTCT 
GTGCATGTGC GGCTGTTGTG GGATGTCTTG ACCCCTGATC CTAACAGCTG CCCACCTCTC 
TACGTGCTCT GGAGGGTCCA CAGCCAGATC CCTCAACGGG TGGTATGGCC AGGCCCAGTA 
CCTTCATGTC TTAGCTTGGC ATTATTGGAG TCTGTGCTGC GTCATGTTGG ACTTAATGAG 
GTGCACAAGG CTGTGGGGCT TCTGCTGGAA ACTCTAGGAC CCCCTCCCAC TGGCCTGCAC 
CTGCAGAGGA GCATCTATCG TGAGATCTTA TTCCTGACAA TGGCAGCTCT GGGCAAGGAT 
CATGTGGACA TAGTGGCCTT CGACAAGAAG TACAAGTCTG CCTTTAACAA GCTGGCCAGC 
AGCATGGGCA AGGAGGAACT AAGGCAACGA CGGGCACAGA TGCCTACTCC CAAGGCGATT 
GACTGCCGAA AATGTTTTGG AGCACCTCTG GAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029413347.1
CDS	149..4747
Translation	MEADAVSEGGAMAEERPPRLVDYFVVAGLAGNGAPIPEETWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGCVPLPMHQGILGGTFRVLYEGKERPKLGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSARTLPRRPYKLLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESIPQYCYDGFPELRAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYHVLREMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKERWQQQQRQQQQQRQRRRQQQQQQMATHQERGSSQTESYLERPSPTRPLQRQTTWAGRSLREPASPAGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEELASGGSPGGSGSALSAQSTEALEGLSGRGPKTGGWQEEAGTPRRGLGARLQQLLTPSRRSSASRIPPPELSPDLPPVARRSPMDSLLWPRERPGSTASESSASLGSEWDLSESSLSSLSLRHSSERLSDTPGPFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTICPFCACPFVPLLSVQTLDSRPSAPSPKSTLASASGSKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHLGGVSRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPGLVLASYDGPPPNQLSQAPSPWLTPDPASVHVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRSIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_029557487.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029557487.1

ORF Insert Sequence:

ATGGAGGCAG ATGCCGTGAG TGAAGGGGGG GCTATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TTGTGGTAGC TGGGCTCGCT GGAAACGGAG CTCCTATACC TGAGGAAACA 
TGGGTTCCTG AACCCACTGG GCCCCTGCGC CCTCCCAGGC CAGCAGAGCC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAAGTGCCCC AGGGTTACAC GTGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGTAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GTGTGTACCC 
CTACCCATGC ACCAGGGAAT ATTGGGAGGG ACCTTTAGGG TGTTGTATGA GGGGAAGGAA 
CGACCCAAGC TTGGTTTTCA AGTGCTCGAC ACGACACCAT ATAGCCACTC GGCCAACCTG 
GCCCCTCCAG GCCCTGGACA CCCCCGCACC TACCTCATGT ACCGGCGGGC AGCAGAGGGG 
GCAGGGCTGC ATGCCCTGGG CATCACTGAC CTCTGCCTGG TACTGCCCAG CAAGGGTGAA 
GGCACTCCTC ATACTTACTG CCGGTTGCCC CGCAATCTTA ACCCTGGCAT GTGGGGCCCA 
GCAGTATACC TGTGCTACAA GGTGGGCCTG GCTAAGGCCA ACACACTGGT GTATGAGGCA 
GAGCTGCTGG GCCGCTACCC AGAGGAGGAC AATGAGGCAT TCCCGCTGCC CGAGTCAGTG 
CCCGTCTTCT GCCTGCCCAT GGGGGCCACT ATCGAGTGCT GGCCTGCCCA GACCAAGTAC 
CCGGTGCCTG TCTTCTCCAC CTTTGTGCTC ACGGGTGCTG CTGGTGATAA GGTGTATGGT 
GCCGCCCTGC AGTTCTACGA AGCGTTCCCT AGGGCCAGGC TGTCAGAGAG GCAGGCACGG 
GCACTGGGCC TGCTGAGTGC TGTGGAACGG GGCCGGGCAC TGGGGGGCCG AGCTGTGCGC 
AGCCGGCGAG CCATTGCTGT ACTGTCCCGC TGGCCTGCCT TCCCAGCCTT CCGAGCCTTC 
CTTACCTTCC TTTACCGCTA CTCGGTCTCA GGCCCTCATC GCCTGCCCTT GGAAGCGCAC 
ATCTCACACT TCATTCACAA CGTCCCATTC CCTTCTCCAC AGAGACCACG CATCCTAGTG 
CAGATGTCTC CCTATGACAA CCTGCTTCTC TGTCAACCTG TGTCCTCACC CCTGCCCCTC 
AGTGGTGCTA GCTTTTTGCA GCTGCTGCAG AGCCTGGGCC CTGAGCTGGC TATTACATTG 
CTGCTGGCTG TGCTCACAGA GCACAAACTG CTGGTCCACT CACTGCGACC AGACCTGCTC 
ACCAGCGTCT GTGAGGCCCT TGTCTCAATG ATCTTCCCGC TGCACTGGCA GTGCCCCTAC 
ATTCCGCTGT GCCCGCTGGT GCTGGCGGAT GTGCTGAGTG CCCCAGTGCC CTTCATTGTG 
GGTATCCACT CCAGCTATTT TGATCTGCAC GACCCACCTG CTGATGTCAT CTGTGTTGAT 
CTTGACACCA ACACACTTTT CCAGACAGAA GAAAAGAAGC CCCTCTCCGC TAGGACCCTG 
CCACGCAGAC CCTACAAGCT TTTACTGGCT ACACTGACAA ATTTGTACCA GCAGCTGGAC 
CAGACTTACA CTGGACCAGA GGAGGAGGCC TCCCTGGAAT TCCTGCTGAC AGACTACGAG 
GCAGTGTGTG GCCGTAGGGC ACGCCTGGAG CGGGAAGTCC AGGGAGCCTT CCTGCGCTTC 
ATGGCCTGTC TGCTCAAAGG CTACCGGGAC TTTCTGCGCC CCCTCACCCA GGCCCCCTCT 
GAGGGCTCTC GTGATGTTGA CAACCTTTTC TACCTTCAGG GTTTCCTCAA GTCCCGGGAA 
CGCTCAAGCC ACAAACTGTA CTCTCAGCTG CTGCACACGC AGATGTTTTC ACAGTTCATT 
GAGGAATGCT CTTTTGGCTC TGCTCGGCAT GCTGCCCTTG AATTCTTTGA CTCTTGTGTT 
GACAAGGTCC ACCCAGAGCA GGAAAAGCCG GAGCCAACAC CCTTAGTGGA GCTGGAGGAG 
CTGTCAGGAA GTGAGCTCAC TGTCTTCATC ACACCCCCAG AGGAGCCTCC AGTACTGGAG 
GGCAGTGAAT CTATACCACA GTACTGTTAC GATGGGTTCC CAGAGCTAAG AGCTGAGCTA 
TTTGAGTCTC CTCAAGAACA ACAGGGGGCC TTGCCTGTGC CAGGCCCATC TCGTAGTGCC 
CCTAGCAGTC CTGCCCCTCG CCGTACCAAA CAGGAGATGA AGGTTGCACA GCGGATGGCA 
CAGAAGTCAG CAGCTGTGCC TGAGCTGTGG GCCCGGTGCC TTCTGGGGCA CTGCTATGGA 
CTGTGGTTCC TCTGCCTGCC TGCCTATGTG CGTTCAGCAC CCTCTCGGGT ACGAGCACTG 
CACACAGCCT ACCATGTTCT GCGTGAGATG GAGAGCCGCA AAGTGGTACT CCCCGATGAG 
GTGTGTTACC GAGTACTGAT GCAGCTCTGC TCACACTATG GGCAGCCTGT GTTGTCTGTT 
CGAGTCATGT TGGAGATGCG GCGGGCTGGC ATCGTGCCCA ACACAATCAC TTATGGTTAC 
TACAACAAGG CTGTGCTGGA AAGCAAGTGG CCGTCTGGTA CACCAGGTGG GCGCCTACGC 
TGGGCCAAGC TCCGGAATGT TGTCCTCGGA GCTGCTCAGT TCCGCCAGCC CTTGAAAGAA 
CGTTGGCAGC AGCAGCAGCG GCAGCAGCAA CAGCAGCGGC AGCGGCGGCG GCAGCAGCAG 
CAGCAACAGA TGGCCACGCA CCAAGAAAGA GGCAGCTCTC AGACAGAGTC CTATCTGGAG 
CGCCCCTCCC CAACCCGCCC TCTTCAGCGC CAGACTACCT GGGCTGGCCG AAGTCTTCGG 
GAACCAGCCT CACCTGCTGG GCGCCTGGTC AAAAGTGGCA GCCTAGGCAG TGCCCGAGGG 
GCACAGCCTA CCGTGGAGGC TGGTGTGGCT CACATGATAG AAGCCTTGGG GGTCCTAGAA 
CCCCGTGGAT CACCTGTGCC TTGGCAAGAT GGAAGCCTTT CAGACCTGAG CCTGACAGGG 
GAAGAGCTGG CATCTGGGGG CAGTCCAGGG GGCTCAGGCT CGGCCCTGAG TGCCCAGTCC 
ACTGAGGCCC TAGAAGGGCT AAGTGGGAGA GGTCCCAAAA CTGGAGGATG GCAGGAGGAG 
GCAGGCACCC CCAGAAGAGG ACTGGGTGCC CGCCTTCAAC AGCTGCTCAC TCCTTCCCGC 
CGCTCCTCTG CCTCCCGCAT CCCCCCACCA GAGTTGTCCC CTGACCTTCC TCCTGTAGCC 
CGTCGCAGTC CCATGGATAG CCTTCTGTGG CCCCGAGAAC GCCCTGGATC CACTGCTTCT 
GAGAGCTCAG CCTCACTGGG CAGTGAGTGG GATCTCTCAG AGTCTTCTCT CAGCAGTCTG 
AGTCTCCGCC ATTCCTCAGA GCGCCTCAGT GACACTCCTG GACCCTTTCA GCCACCTTCC 
CTGGAAATTC TGCTGTCTAG CTGTTCCTTG TGTCGTGCCT GTGATTCACT GGTGTATGAT 
GAGGAAATCA TGGCTGGCTG GGCACCTGAT GACTCCAACC TCAACACAAT TTGCCCCTTC 
TGCGCCTGCC CGTTTGTGCC GCTCCTCAGT GTCCAGACCC TTGATTCCCG ACCTAGTGCC 
CCTAGCCCTA AGTCTACCCT TGCCAGTGCC AGTGGTAGCA AAGATGCTCC TGTCCCTGGG 
GGTCCTGGCC CTGTGCTCAG TGACCGAAGG CTCTGTCTTG CCTTGGATGA ACCCCAGCTC 
TGCAATGGAC ATTTGGGGGG TGTCTCCCGG CGGGTTGAGA GTGGGGCATG GGCATACCTA 
AGCCCCCTGG TGCTGCGTAA GGAGCTAGAG TCGCTGGTAG AGAATGAAGG CAGCGAGGTG 
TTGGCATTGC CTGAACTGCC TGCTGCCCAC CCCATCATTT TCTGGAACCT TCTGTGGTAT 
TTCCAACGGC TACGCCTGCC TAGTATTCTA CCAGGCCTAG TGCTGGCCTC TTATGATGGG 
CCCCCACCCA ACCAGCTCTC CCAGGCACCA TCTCCTTGGT TAACCCCTGA TCCAGCCTCT 
GTGCATGTGC GGCTGTTGTG GGATGTCTTG ACCCCTGATC CTAACAGCTG CCCACCTCTC 
TACGTGCTCT GGAGGGTCCA CAGCCAGATC CCTCAACGGG TGGTATGGCC AGGCCCAGTA 
CCTTCATGTC TTAGCTTGGC ATTATTGGAG TCTGTGCTGC GTCATGTTGG ACTTAATGAG 
GTGCACAAGG CTGTGGGGCT TCTGCTGGAA ACTCTAGGAC CCCCTCCCAC TGGCCTGCAC 
CTGCAGAGGA GCATCTATCG TGAGATCTTA TTCCTGACAA TGGCAGCTCT GGGCAAGGAT 
CATGTGGACA TAGTGGCCTT CGACAAGAAG TACAAGTCTG CCTTTAACAA GCTGGCCAGC 
AGCATGGGCA AGGAGGAACT AAGGCAACGA CGGGCACAGA TGCCTACTCC CAAGGCGATT 
GACTGCCGAA AATGTTTTGG AGCACCTCTG GAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_008855239.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008853461.2 Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X1, mRNA.

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CloneID	ONa43209
Clone ID Related Accession (Same CDS sequence)	XM_008855239.2
Accession Version	XM_008855239.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	DENN domain-containing protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008330926.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008855239.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAG CAGCCTCTCT TGCTCCATCT TTTCCTCAAT TGATCATTCC AGATGCCGTG 
AGTGAAGGGG GGGCTATGGC GGAGGAGCGG CCCCCCCGGC TGGTGGATTA CTTTGTGGTA 
GCTGGGCTCG CTGGAAACGG AGCTCCTATA CCTGAGGAAA CATGGGTTCC TGAACCCACT 
GGGCCCCTGC GCCCTCCCAG GCCAGCAGAG CCTATCACAG ATGTGGCAGT CATCGCTAGG 
GCACTGGGCG AGGAAGTGCC CCAGGGTTAC ACGTGCATCC AGGCTTCTGC TGGGGGCCAC 
CCCTTGGAAC TCAGTGCTGG GCTCCTGGGT GGAACTCAAC CAGTCATCTG CTACCGTAGG 
GGGCGTGACA AGCCCCCCCT CGTTGAGCTG GGGGTGTTGT ATGAGGGGAA GGAACGACCC 
AAGCTTGGTT TTCAAGTGCT CGACACGACA CCATATAGCC ACTCGGCCAA CCTGGCCCCT 
CCAGGCCCTG GACACCCCCG CACCTACCTC ATGTACCGGC GGGCAGCAGA GGGGGCAGGG 
CTGCATGCCC TGGGCATCAC TGACCTCTGC CTGGTACTGC CCAGCAAGGG TGAAGGCACT 
CCTCATACTT ACTGCCGGTT GCCCCGCAAT CTTAACCCTG GCATGTGGGG CCCAGCAGTA 
TACCTGTGCT ACAAGGTGGG CCTGGCTAAG GCCAACACAC TGGTGTATGA GGCAGAGCTG 
CTGGGCCGCT ACCCAGAGGA GGACAATGAG GCATTCCCGC TGCCCGAGTC AGTGCCCGTC 
TTCTGCCTGC CCATGGGGGC CACTATCGAG TGCTGGCCTG CCCAGACCAA GTACCCGGTG 
CCTGTCTTCT CCACCTTTGT GCTCACGGGT GCTGCTGGTG ATAAGGTGTA TGGTGCCGCC 
CTGCAGTTCT ACGAAGCGTT CCCTAGGGCC AGGCTGTCAG AGAGGCAGGC ACGGGCACTG 
GGCCTGCTGA GTGCTGTGGA ACGGGGCCGG GCACTGGGGG GCCGAGCTGT GCGCAGCCGG 
CGAGCCATTG CTGTACTGTC CCGCTGGCCT GCCTTCCCAG CCTTCCGAGC CTTCCTTACC 
TTCCTTTACC GCTACTCGGT CTCAGGCCCT CATCGCCTGC CCTTGGAAGC GCACATCTCA 
CACTTCATTC ACAACGTCCC ATTCCCTTCT CCACAGAGAC CACGCATCCT AGTGCAGATG 
TCTCCCTATG ACAACCTGCT TCTCTGTCAA CCTGTGTCCT CACCCCTGCC CCTCAGTGGT 
GCTAGCTTTT TGCAGCTGCT GCAGAGCCTG GGCCCTGAGC TGGCTATTAC ATTGCTGCTG 
GCTGTGCTCA CAGAGCACAA ACTGCTGGTC CACTCACTGC GACCAGACCT GCTCACCAGC 
GTCTGTGAGG CCCTTGTCTC AATGATCTTC CCGCTGCACT GGCAGTGCCC CTACATTCCG 
CTGTGCCCGC TGGTGCTGGC GGATGTGCTG AGTGCCCCAG TGCCCTTCAT TGTGGGTATC 
CACTCCAGCT ATTTTGATCT GCACGACCCA CCTGCTGATG TCATCTGTGT TGATCTTGAC 
ACCAACACAC TTTTCCAGAC AGAAGAAAAG AAGCCCCTCT CCGCTAGGAC CCTGCCACGC 
AGACCCTACA AGCTTTTACT GGCTACACTG ACAAATTTGT ACCAGCAGCT GGACCAGACT 
TACACTGGAC CAGAGGAGGA GGCCTCCCTG GAATTCCTGC TGACAGACTA CGAGGCAGTG 
TGTGGCCGTA GGGCACGCCT GGAGCGGGAA GTCCAGGGAG CCTTCCTGCG CTTCATGGCC 
TGTCTGCTCA AAGGCTACCG GGACTTTCTG CGCCCCCTCA CCCAGGCCCC CTCTGAGGGC 
TCTCGTGATG TTGACAACCT TTTCTACCTT CAGGGTTTCC TCAAGTCCCG GGAACGCTCA 
AGCCACAAAC TGTACTCTCA GCTGCTGCAC ACGCAGATGT TTTCACAGTT CATTGAGGAA 
TGCTCTTTTG GCTCTGCTCG GCATGCTGCC CTTGAATTCT TTGACTCTTG TGTTGACAAG 
GTCCACCCAG AGCAGGAAAA GCCGGAGCCA ACACCCTTAG TGGAGCTGGA GGAGCTGTCA 
GGAAGTGAGC TCACTGTCTT CATCACACCC CCAGAGGAGC CTCCAGTACT GGAGGGCAGT 
GAATCTATAC CACAGTACTG TTACGATGGG TTCCCAGAGC TAAGAGCTGA GCTATTTGAG 
TCTCCTCAAG AACAACAGGG GGCCTTGCCT GTGCCAGGCC CATCTCGTAG TGCCCCTAGC 
AGTCCTGCCC CTCGCCGTAC CAAACAGGAG ATGAAGGTTG CACAGCGGAT GGCACAGAAG 
TCAGCAGCTG TGCCTGAGCT GTGGGCCCGG TGCCTTCTGG GGCACTGCTA TGGACTGTGG 
TTCCTCTGCC TGCCTGCCTA TGTGCGTTCA GCACCCTCTC GGGTACGAGC ACTGCACACA 
GCCTACCATG TTCTGCGTGA GATGGAGAGC CGCAAAGTGG TACTCCCCGA TGAGGTGTGT 
TACCGAGTAC TGATGCAGCT CTGCTCACAC TATGGGCAGC CTGTGTTGTC TGTTCGAGTC 
ATGTTGGAGA TGCGGCGGGC TGGCATCGTG CCCAACACAA TCACTTATGG TTACTACAAC 
AAGGCTGTGC TGGAAAGCAA GTGGCCGTCT GGTACACCAG GTGGGCGCCT ACGCTGGGCC 
AAGCTCCGGA ATGTTGTCCT CGGAGCTGCT CAGTTCCGCC AGCCCTTGAA AGAACGTTGG 
CAGCAGCAGC AGCGGCAGCA GCAACAGCAG CGGCAGCGGC GGCGGCAGCA GCAGCAGCAA 
CAGATGGCCA CGCACCAAGA AAGAGGCAGC TCTCAGACAG AGTCCTATCT GGAGCGCCCC 
TCCCCAACCC GCCCTCTTCA GCGCCAGACT ACCTGGGCTG GCCGAAGTCT TCGGGAACCA 
GCCTCACCTG CTGGGCGCCT GGTCAAAAGT GGCAGCCTAG GCAGTGCCCG AGGGGCACAG 
CCTACCGTGG AGGCTGGTGT GGCTCACATG ATAGAAGCCT TGGGGGTCCT AGAACCCCGT 
GGATCACCTG TGCCTTGGCA AGATGGAAGC CTTTCAGACC TGAGCCTGAC AGGGGAAGAG 
CTGGCATCTG GGGGCAGTCC AGGGGGCTCA GGCTCGGCCC TGAGTGCCCA GTCCACTGAG 
GCCCTAGAAG GGCTAAGTGG GAGAGGTCCC AAAACTGGAG GATGGCAGGA GGAGGCAGGC 
ACCCCCAGAA GAGGACTGGG TGCCCGCCTT CAACAGCTGC TCACTCCTTC CCGCCGCTCC 
TCTGCCTCCC GCATCCCCCC ACCAGAGTTG TCCCCTGACC TTCCTCCTGT AGCCCGTCGC 
AGTCCCATGG ATAGCCTTCT GTGGCCCCGA GAACGCCCTG GATCCACTGC TTCTGAGAGC 
TCAGCCTCAC TGGGCAGTGA GTGGGATCTC TCAGAGTCTT CTCTCAGCAG TCTGAGTCTC 
CGCCATTCCT CAGAGCGCCT CAGTGACACT CCTGGACCCT TTCAGCCACC TTCCCTGGAA 
ATTCTGCTGT CTAGCTGTTC CTTGTGTCGT GCCTGTGATT CACTGGTGTA TGATGAGGAA 
ATCATGGCTG GCTGGGCACC TGATGACTCC AACCTCAACA CAATTTGCCC CTTCTGCGCC 
TGCCCGTTTG TGCCGCTCCT CAGTGTCCAG ACCCTTGATT CCCGACCTAG TGCCCCTAGC 
CCTAAGTCTA CCCTTGCCAG TGCCAGTGGT AGCAAAGATG CTCCTGTCCC TGGGGGTCCT 
GGCCCTGTGC TCAGTGACCG AAGGCTCTGT CTTGCCTTGG ATGAACCCCA GCTCTGCAAT 
GGACATTTGG GGGGTGTCTC CCGGCGGGTT GAGAGTGGGG CATGGGCATA CCTAAGCCCC 
CTGGTGCTGC GTAAGGAGCT AGAGTCGCTG GTAGAGAATG AAGGCAGCGA GGTGTTGGCA 
TTGCCTGAAC TGCCTGCTGC CCACCCCATC ATTTTCTGGA ACCTTCTGTG GTATTTCCAA 
CGGCTACGCC TGCCTAGTAT TCTACCAGGC CTAGTGCTGG CCTCTTATGA TGGGCCCCCA 
CCCAACCAGC TCTCCCAGGC ACCATCTCCT TGGTTAACCC CTGATCCAGC CTCTGTGCAT 
GTGCGGCTGT TGTGGGATGT CTTGACCCCT GATCCTAACA GCTGCCCACC TCTCTACGTG 
CTCTGGAGGG TCCACAGCCA GATCCCTCAA CGGGTGGTAT GGCCAGGCCC AGTACCTTCA 
TGTCTTAGCT TGGCATTATT GGAGTCTGTG CTGCGTCATG TTGGACTTAA TGAGGTGCAC 
AAGGCTGTGG GGCTTCTGCT GGAAACTCTA GGACCCCCTC CCACTGGCCT GCACCTGCAG 
AGGAGCATCT ATCGTGAGAT CTTATTCCTG ACAATGGCAG CTCTGGGCAA GGATCATGTG 
GACATAGTGG CCTTCGACAA GAAGTACAAG TCTGCCTTTA ACAAGCTGGC CAGCAGCATG 
GGCAAGGAGG AACTAAGGCA ACGACGGGCA CAGATGCCTA CTCCCAAGGC GATTGACTGC 
CGAAAATGTT TTGGAGCACC TCTGGAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008853461.2
CDS	17..4609
Translation	METAASLAPSFPQLIIPDAVSEGGAMAEERPPRLVDYFVVAGLAGNGAPIPEETWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKLGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSARTLPRRPYKLLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESIPQYCYDGFPELRAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYHVLREMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKERWQQQQRQQQQQRQRRRQQQQQQMATHQERGSSQTESYLERPSPTRPLQRQTTWAGRSLREPASPAGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEELASGGSPGGSGSALSAQSTEALEGLSGRGPKTGGWQEEAGTPRRGLGARLQQLLTPSRRSSASRIPPPELSPDLPPVARRSPMDSLLWPRERPGSTASESSASLGSEWDLSESSLSSLSLRHSSERLSDTPGPFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTICPFCACPFVPLLSVQTLDSRPSAPSPKSTLASASGSKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHLGGVSRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPGLVLASYDGPPPNQLSQAPSPWLTPDPASVHVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRSIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_008855239.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008855239.2

ORF Insert Sequence:

ATGGAAACAG CAGCCTCTCT TGCTCCATCT TTTCCTCAAT TGATCATTCC AGATGCCGTG 
AGTGAAGGGG GGGCTATGGC GGAGGAGCGG CCCCCCCGGC TGGTGGATTA CTTTGTGGTA 
GCTGGGCTCG CTGGAAACGG AGCTCCTATA CCTGAGGAAA CATGGGTTCC TGAACCCACT 
GGGCCCCTGC GCCCTCCCAG GCCAGCAGAG CCTATCACAG ATGTGGCAGT CATCGCTAGG 
GCACTGGGCG AGGAAGTGCC CCAGGGTTAC ACGTGCATCC AGGCTTCTGC TGGGGGCCAC 
CCCTTGGAAC TCAGTGCTGG GCTCCTGGGT GGAACTCAAC CAGTCATCTG CTACCGTAGG 
GGGCGTGACA AGCCCCCCCT CGTTGAGCTG GGGGTGTTGT ATGAGGGGAA GGAACGACCC 
AAGCTTGGTT TTCAAGTGCT CGACACGACA CCATATAGCC ACTCGGCCAA CCTGGCCCCT 
CCAGGCCCTG GACACCCCCG CACCTACCTC ATGTACCGGC GGGCAGCAGA GGGGGCAGGG 
CTGCATGCCC TGGGCATCAC TGACCTCTGC CTGGTACTGC CCAGCAAGGG TGAAGGCACT 
CCTCATACTT ACTGCCGGTT GCCCCGCAAT CTTAACCCTG GCATGTGGGG CCCAGCAGTA 
TACCTGTGCT ACAAGGTGGG CCTGGCTAAG GCCAACACAC TGGTGTATGA GGCAGAGCTG 
CTGGGCCGCT ACCCAGAGGA GGACAATGAG GCATTCCCGC TGCCCGAGTC AGTGCCCGTC 
TTCTGCCTGC CCATGGGGGC CACTATCGAG TGCTGGCCTG CCCAGACCAA GTACCCGGTG 
CCTGTCTTCT CCACCTTTGT GCTCACGGGT GCTGCTGGTG ATAAGGTGTA TGGTGCCGCC 
CTGCAGTTCT ACGAAGCGTT CCCTAGGGCC AGGCTGTCAG AGAGGCAGGC ACGGGCACTG 
GGCCTGCTGA GTGCTGTGGA ACGGGGCCGG GCACTGGGGG GCCGAGCTGT GCGCAGCCGG 
CGAGCCATTG CTGTACTGTC CCGCTGGCCT GCCTTCCCAG CCTTCCGAGC CTTCCTTACC 
TTCCTTTACC GCTACTCGGT CTCAGGCCCT CATCGCCTGC CCTTGGAAGC GCACATCTCA 
CACTTCATTC ACAACGTCCC ATTCCCTTCT CCACAGAGAC CACGCATCCT AGTGCAGATG 
TCTCCCTATG ACAACCTGCT TCTCTGTCAA CCTGTGTCCT CACCCCTGCC CCTCAGTGGT 
GCTAGCTTTT TGCAGCTGCT GCAGAGCCTG GGCCCTGAGC TGGCTATTAC ATTGCTGCTG 
GCTGTGCTCA CAGAGCACAA ACTGCTGGTC CACTCACTGC GACCAGACCT GCTCACCAGC 
GTCTGTGAGG CCCTTGTCTC AATGATCTTC CCGCTGCACT GGCAGTGCCC CTACATTCCG 
CTGTGCCCGC TGGTGCTGGC GGATGTGCTG AGTGCCCCAG TGCCCTTCAT TGTGGGTATC 
CACTCCAGCT ATTTTGATCT GCACGACCCA CCTGCTGATG TCATCTGTGT TGATCTTGAC 
ACCAACACAC TTTTCCAGAC AGAAGAAAAG AAGCCCCTCT CCGCTAGGAC CCTGCCACGC 
AGACCCTACA AGCTTTTACT GGCTACACTG ACAAATTTGT ACCAGCAGCT GGACCAGACT 
TACACTGGAC CAGAGGAGGA GGCCTCCCTG GAATTCCTGC TGACAGACTA CGAGGCAGTG 
TGTGGCCGTA GGGCACGCCT GGAGCGGGAA GTCCAGGGAG CCTTCCTGCG CTTCATGGCC 
TGTCTGCTCA AAGGCTACCG GGACTTTCTG CGCCCCCTCA CCCAGGCCCC CTCTGAGGGC 
TCTCGTGATG TTGACAACCT TTTCTACCTT CAGGGTTTCC TCAAGTCCCG GGAACGCTCA 
AGCCACAAAC TGTACTCTCA GCTGCTGCAC ACGCAGATGT TTTCACAGTT CATTGAGGAA 
TGCTCTTTTG GCTCTGCTCG GCATGCTGCC CTTGAATTCT TTGACTCTTG TGTTGACAAG 
GTCCACCCAG AGCAGGAAAA GCCGGAGCCA ACACCCTTAG TGGAGCTGGA GGAGCTGTCA 
GGAAGTGAGC TCACTGTCTT CATCACACCC CCAGAGGAGC CTCCAGTACT GGAGGGCAGT 
GAATCTATAC CACAGTACTG TTACGATGGG TTCCCAGAGC TAAGAGCTGA GCTATTTGAG 
TCTCCTCAAG AACAACAGGG GGCCTTGCCT GTGCCAGGCC CATCTCGTAG TGCCCCTAGC 
AGTCCTGCCC CTCGCCGTAC CAAACAGGAG ATGAAGGTTG CACAGCGGAT GGCACAGAAG 
TCAGCAGCTG TGCCTGAGCT GTGGGCCCGG TGCCTTCTGG GGCACTGCTA TGGACTGTGG 
TTCCTCTGCC TGCCTGCCTA TGTGCGTTCA GCACCCTCTC GGGTACGAGC ACTGCACACA 
GCCTACCATG TTCTGCGTGA GATGGAGAGC CGCAAAGTGG TACTCCCCGA TGAGGTGTGT 
TACCGAGTAC TGATGCAGCT CTGCTCACAC TATGGGCAGC CTGTGTTGTC TGTTCGAGTC 
ATGTTGGAGA TGCGGCGGGC TGGCATCGTG CCCAACACAA TCACTTATGG TTACTACAAC 
AAGGCTGTGC TGGAAAGCAA GTGGCCGTCT GGTACACCAG GTGGGCGCCT ACGCTGGGCC 
AAGCTCCGGA ATGTTGTCCT CGGAGCTGCT CAGTTCCGCC AGCCCTTGAA AGAACGTTGG 
CAGCAGCAGC AGCGGCAGCA GCAACAGCAG CGGCAGCGGC GGCGGCAGCA GCAGCAGCAA 
CAGATGGCCA CGCACCAAGA AAGAGGCAGC TCTCAGACAG AGTCCTATCT GGAGCGCCCC 
TCCCCAACCC GCCCTCTTCA GCGCCAGACT ACCTGGGCTG GCCGAAGTCT TCGGGAACCA 
GCCTCACCTG CTGGGCGCCT GGTCAAAAGT GGCAGCCTAG GCAGTGCCCG AGGGGCACAG 
CCTACCGTGG AGGCTGGTGT GGCTCACATG ATAGAAGCCT TGGGGGTCCT AGAACCCCGT 
GGATCACCTG TGCCTTGGCA AGATGGAAGC CTTTCAGACC TGAGCCTGAC AGGGGAAGAG 
CTGGCATCTG GGGGCAGTCC AGGGGGCTCA GGCTCGGCCC TGAGTGCCCA GTCCACTGAG 
GCCCTAGAAG GGCTAAGTGG GAGAGGTCCC AAAACTGGAG GATGGCAGGA GGAGGCAGGC 
ACCCCCAGAA GAGGACTGGG TGCCCGCCTT CAACAGCTGC TCACTCCTTC CCGCCGCTCC 
TCTGCCTCCC GCATCCCCCC ACCAGAGTTG TCCCCTGACC TTCCTCCTGT AGCCCGTCGC 
AGTCCCATGG ATAGCCTTCT GTGGCCCCGA GAACGCCCTG GATCCACTGC TTCTGAGAGC 
TCAGCCTCAC TGGGCAGTGA GTGGGATCTC TCAGAGTCTT CTCTCAGCAG TCTGAGTCTC 
CGCCATTCCT CAGAGCGCCT CAGTGACACT CCTGGACCCT TTCAGCCACC TTCCCTGGAA 
ATTCTGCTGT CTAGCTGTTC CTTGTGTCGT GCCTGTGATT CACTGGTGTA TGATGAGGAA 
ATCATGGCTG GCTGGGCACC TGATGACTCC AACCTCAACA CAATTTGCCC CTTCTGCGCC 
TGCCCGTTTG TGCCGCTCCT CAGTGTCCAG ACCCTTGATT CCCGACCTAG TGCCCCTAGC 
CCTAAGTCTA CCCTTGCCAG TGCCAGTGGT AGCAAAGATG CTCCTGTCCC TGGGGGTCCT 
GGCCCTGTGC TCAGTGACCG AAGGCTCTGT CTTGCCTTGG ATGAACCCCA GCTCTGCAAT 
GGACATTTGG GGGGTGTCTC CCGGCGGGTT GAGAGTGGGG CATGGGCATA CCTAAGCCCC 
CTGGTGCTGC GTAAGGAGCT AGAGTCGCTG GTAGAGAATG AAGGCAGCGA GGTGTTGGCA 
TTGCCTGAAC TGCCTGCTGC CCACCCCATC ATTTTCTGGA ACCTTCTGTG GTATTTCCAA 
CGGCTACGCC TGCCTAGTAT TCTACCAGGC CTAGTGCTGG CCTCTTATGA TGGGCCCCCA 
CCCAACCAGC TCTCCCAGGC ACCATCTCCT TGGTTAACCC CTGATCCAGC CTCTGTGCAT 
GTGCGGCTGT TGTGGGATGT CTTGACCCCT GATCCTAACA GCTGCCCACC TCTCTACGTG 
CTCTGGAGGG TCCACAGCCA GATCCCTCAA CGGGTGGTAT GGCCAGGCCC AGTACCTTCA 
TGTCTTAGCT TGGCATTATT GGAGTCTGTG CTGCGTCATG TTGGACTTAA TGAGGTGCAC 
AAGGCTGTGG GGCTTCTGCT GGAAACTCTA GGACCCCCTC CCACTGGCCT GCACCTGCAG 
AGGAGCATCT ATCGTGAGAT CTTATTCCTG ACAATGGCAG CTCTGGGCAA GGATCATGTG 
GACATAGTGG CCTTCGACAA GAAGTACAAG TCTGCCTTTA ACAAGCTGGC CAGCAGCATG 
GGCAAGGAGG AACTAAGGCA ACGACGGGCA CAGATGCCTA CTCCCAAGGC GATTGACTGC 
CGAAAATGTT TTGGAGCACC TCTGGAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_017795122.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017650611.1 Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X2, mRNA.

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CloneID	ONa43210
Clone ID Related Accession (Same CDS sequence)	XM_017795122.2 , XM_017795122.1
Accession Version	XM_017795122.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008330926.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_017795122.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCCGTGAG TGAAGGGGGG GCTATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TTGTGGTAGC TGGGCTCGCT GGAAACGGAG CTCCTATACC TGAGGAAACA 
TGGGTTCCTG AACCCACTGG GCCCCTGCGC CCTCCCAGGC CAGCAGAGCC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAAGTGCCCC AGGGTTACAC GTGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGTAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGACCCAA GCTTGGTTTT CAAGTGCTCG ACACGACACC ATATAGCCAC 
TCGGCCAACC TGGCCCCTCC AGGCCCTGGA CACCCCCGCA CCTACCTCAT GTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGTG AAGGCACTCC TCATACTTAC TGCCGGTTGC CCCGCAATCT TAACCCTGGC 
ATGTGGGGCC CAGCAGTATA CCTGTGCTAC AAGGTGGGCC TGGCTAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC ATTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCGGTGCC TGTCTTCTCC ACCTTTGTGC TCACGGGTGC TGCTGGTGAT 
AAGGTGTATG GTGCCGCCCT GCAGTTCTAC GAAGCGTTCC CTAGGGCCAG GCTGTCAGAG 
AGGCAGGCAC GGGCACTGGG CCTGCTGAGT GCTGTGGAAC GGGGCCGGGC ACTGGGGGGC 
CGAGCTGTGC GCAGCCGGCG AGCCATTGCT GTACTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGAGCCT TCCTTACCTT CCTTTACCGC TACTCGGTCT CAGGCCCTCA TCGCCTGCCC 
TTGGAAGCGC ACATCTCACA CTTCATTCAC AACGTCCCAT TCCCTTCTCC ACAGAGACCA 
CGCATCCTAG TGCAGATGTC TCCCTATGAC AACCTGCTTC TCTGTCAACC TGTGTCCTCA 
CCCCTGCCCC TCAGTGGTGC TAGCTTTTTG CAGCTGCTGC AGAGCCTGGG CCCTGAGCTG 
GCTATTACAT TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTGGTCCA CTCACTGCGA 
CCAGACCTGC TCACCAGCGT CTGTGAGGCC CTTGTCTCAA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCGCTG GTGCTGGCGG ATGTGCTGAG TGCCCCAGTG 
CCCTTCATTG TGGGTATCCA CTCCAGCTAT TTTGATCTGC ACGACCCACC TGCTGATGTC 
ATCTGTGTTG ATCTTGACAC CAACACACTT TTCCAGACAG AAGAAAAGAA GCCCCTCTCC 
GCTAGGACCC TGCCACGCAG ACCCTACAAG CTTTTACTGG CTACACTGAC AAATTTGTAC 
CAGCAGCTGG ACCAGACTTA CACTGGACCA GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACAGACTACG AGGCAGTGTG TGGCCGTAGG GCACGCCTGG AGCGGGAAGT CCAGGGAGCC 
TTCCTGCGCT TCATGGCCTG TCTGCTCAAA GGCTACCGGG ACTTTCTGCG CCCCCTCACC 
CAGGCCCCCT CTGAGGGCTC TCGTGATGTT GACAACCTTT TCTACCTTCA GGGTTTCCTC 
AAGTCCCGGG AACGCTCAAG CCACAAACTG TACTCTCAGC TGCTGCACAC GCAGATGTTT 
TCACAGTTCA TTGAGGAATG CTCTTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TTGACAAGGT CCACCCAGAG CAGGAAAAGC CGGAGCCAAC ACCCTTAGTG 
GAGCTGGAGG AGCTGTCAGG AAGTGAGCTC ACTGTCTTCA TCACACCCCC AGAGGAGCCT 
CCAGTACTGG AGGGCAGTGA ATCTATACCA CAGTACTGTT ACGATGGGTT CCCAGAGCTA 
AGAGCTGAGC TATTTGAGTC TCCTCAAGAA CAACAGGGGG CCTTGCCTGT GCCAGGCCCA 
TCTCGTAGTG CCCCTAGCAG TCCTGCCCCT CGCCGTACCA AACAGGAGAT GAAGGTTGCA 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTTCTGGGG 
CACTGCTATG GACTGTGGTT CCTCTGCCTG CCTGCCTATG TGCGTTCAGC ACCCTCTCGG 
GTACGAGCAC TGCACACAGC CTACCATGTT CTGCGTGAGA TGGAGAGCCG CAAAGTGGTA 
CTCCCCGATG AGGTGTGTTA CCGAGTACTG ATGCAGCTCT GCTCACACTA TGGGCAGCCT 
GTGTTGTCTG TTCGAGTCAT GTTGGAGATG CGGCGGGCTG GCATCGTGCC CAACACAATC 
ACTTATGGTT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG TACACCAGGT 
GGGCGCCTAC GCTGGGCCAA GCTCCGGAAT GTTGTCCTCG GAGCTGCTCA GTTCCGCCAG 
CCCTTGAAAG AACGTTGGCA GCAGCAGCAG CGGCAGCAGC AACAGCAGCG GCAGCGGCGG 
CGGCAGCAGC AGCAGCAACA GATGGCCACG CACCAAGAAA GAGGCAGCTC TCAGACAGAG 
TCCTATCTGG AGCGCCCCTC CCCAACCCGC CCTCTTCAGC GCCAGACTAC CTGGGCTGGC 
CGAAGTCTTC GGGAACCAGC CTCACCTGCT GGGCGCCTGG TCAAAAGTGG CAGCCTAGGC 
AGTGCCCGAG GGGCACAGCC TACCGTGGAG GCTGGTGTGG CTCACATGAT AGAAGCCTTG 
GGGGTCCTAG AACCCCGTGG ATCACCTGTG CCTTGGCAAG ATGGAAGCCT TTCAGACCTG 
AGCCTGACAG GGGAAGAGCT GGCATCTGGG GGCAGTCCAG GGGGCTCAGG CTCGGCCCTG 
AGTGCCCAGT CCACTGAGGC CCTAGAAGGG CTAAGTGGGA GAGGTCCCAA AACTGGAGGA 
TGGCAGGAGG AGGCAGGCAC CCCCAGAAGA GGACTGGGTG CCCGCCTTCA ACAGCTGCTC 
ACTCCTTCCC GCCGCTCCTC TGCCTCCCGC ATCCCCCCAC CAGAGTTGTC CCCTGACCTT 
CCTCCTGTAG CCCGTCGCAG TCCCATGGAT AGCCTTCTGT GGCCCCGAGA ACGCCCTGGA 
TCCACTGCTT CTGAGAGCTC AGCCTCACTG GGCAGTGAGT GGGATCTCTC AGAGTCTTCT 
CTCAGCAGTC TGAGTCTCCG CCATTCCTCA GAGCGCCTCA GTGACACTCC TGGACCCTTT 
CAGCCACCTT CCCTGGAAAT TCTGCTGTCT AGCTGTTCCT TGTGTCGTGC CTGTGATTCA 
CTGGTGTATG ATGAGGAAAT CATGGCTGGC TGGGCACCTG ATGACTCCAA CCTCAACACA 
ATTTGCCCCT TCTGCGCCTG CCCGTTTGTG CCGCTCCTCA GTGTCCAGAC CCTTGATTCC 
CGACCTAGTG CCCCTAGCCC TAAGTCTACC CTTGCCAGTG CCAGTGGTAG CAAAGATGCT 
CCTGTCCCTG GGGGTCCTGG CCCTGTGCTC AGTGACCGAA GGCTCTGTCT TGCCTTGGAT 
GAACCCCAGC TCTGCAATGG ACATTTGGGG GGTGTCTCCC GGCGGGTTGA GAGTGGGGCA 
TGGGCATACC TAAGCCCCCT GGTGCTGCGT AAGGAGCTAG AGTCGCTGGT AGAGAATGAA 
GGCAGCGAGG TGTTGGCATT GCCTGAACTG CCTGCTGCCC ACCCCATCAT TTTCTGGAAC 
CTTCTGTGGT ATTTCCAACG GCTACGCCTG CCTAGTATTC TACCAGGCCT AGTGCTGGCC 
TCTTATGATG GGCCCCCACC CAACCAGCTC TCCCAGGCAC CATCTCCTTG GTTAACCCCT 
GATCCAGCCT CTGTGCATGT GCGGCTGTTG TGGGATGTCT TGACCCCTGA TCCTAACAGC 
TGCCCACCTC TCTACGTGCT CTGGAGGGTC CACAGCCAGA TCCCTCAACG GGTGGTATGG 
CCAGGCCCAG TACCTTCATG TCTTAGCTTG GCATTATTGG AGTCTGTGCT GCGTCATGTT 
GGACTTAATG AGGTGCACAA GGCTGTGGGG CTTCTGCTGG AAACTCTAGG ACCCCCTCCC 
ACTGGCCTGC ACCTGCAGAG GAGCATCTAT CGTGAGATCT TATTCCTGAC AATGGCAGCT 
CTGGGCAAGG ATCATGTGGA CATAGTGGCC TTCGACAAGA AGTACAAGTC TGCCTTTAAC 
AAGCTGGCCA GCAGCATGGG CAAGGAGGAA CTAAGGCAAC GACGGGCACA GATGCCTACT 
CCCAAGGCGA TTGACTGCCG AAAATGTTTT GGAGCACCTC TGGAATGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017650611.1
CDS	149..4699
Translation	MEADAVSEGGAMAEERPPRLVDYFVVAGLAGNGAPIPEETWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKLGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSARTLPRRPYKLLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESIPQYCYDGFPELRAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYHVLREMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKERWQQQQRQQQQQRQRRRQQQQQQMATHQERGSSQTESYLERPSPTRPLQRQTTWAGRSLREPASPAGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEELASGGSPGGSGSALSAQSTEALEGLSGRGPKTGGWQEEAGTPRRGLGARLQQLLTPSRRSSASRIPPPELSPDLPPVARRSPMDSLLWPRERPGSTASESSASLGSEWDLSESSLSSLSLRHSSERLSDTPGPFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTICPFCACPFVPLLSVQTLDSRPSAPSPKSTLASASGSKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHLGGVSRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPGLVLASYDGPPPNQLSQAPSPWLTPDPASVHVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRSIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_017795122.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017795122.2

ORF Insert Sequence:

ATGGAGGCAG ATGCCGTGAG TGAAGGGGGG GCTATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TTGTGGTAGC TGGGCTCGCT GGAAACGGAG CTCCTATACC TGAGGAAACA 
TGGGTTCCTG AACCCACTGG GCCCCTGCGC CCTCCCAGGC CAGCAGAGCC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAAGTGCCCC AGGGTTACAC GTGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGTAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGACCCAA GCTTGGTTTT CAAGTGCTCG ACACGACACC ATATAGCCAC 
TCGGCCAACC TGGCCCCTCC AGGCCCTGGA CACCCCCGCA CCTACCTCAT GTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGTG AAGGCACTCC TCATACTTAC TGCCGGTTGC CCCGCAATCT TAACCCTGGC 
ATGTGGGGCC CAGCAGTATA CCTGTGCTAC AAGGTGGGCC TGGCTAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC ATTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCGGTGCC TGTCTTCTCC ACCTTTGTGC TCACGGGTGC TGCTGGTGAT 
AAGGTGTATG GTGCCGCCCT GCAGTTCTAC GAAGCGTTCC CTAGGGCCAG GCTGTCAGAG 
AGGCAGGCAC GGGCACTGGG CCTGCTGAGT GCTGTGGAAC GGGGCCGGGC ACTGGGGGGC 
CGAGCTGTGC GCAGCCGGCG AGCCATTGCT GTACTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGAGCCT TCCTTACCTT CCTTTACCGC TACTCGGTCT CAGGCCCTCA TCGCCTGCCC 
TTGGAAGCGC ACATCTCACA CTTCATTCAC AACGTCCCAT TCCCTTCTCC ACAGAGACCA 
CGCATCCTAG TGCAGATGTC TCCCTATGAC AACCTGCTTC TCTGTCAACC TGTGTCCTCA 
CCCCTGCCCC TCAGTGGTGC TAGCTTTTTG CAGCTGCTGC AGAGCCTGGG CCCTGAGCTG 
GCTATTACAT TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTGGTCCA CTCACTGCGA 
CCAGACCTGC TCACCAGCGT CTGTGAGGCC CTTGTCTCAA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCGCTG GTGCTGGCGG ATGTGCTGAG TGCCCCAGTG 
CCCTTCATTG TGGGTATCCA CTCCAGCTAT TTTGATCTGC ACGACCCACC TGCTGATGTC 
ATCTGTGTTG ATCTTGACAC CAACACACTT TTCCAGACAG AAGAAAAGAA GCCCCTCTCC 
GCTAGGACCC TGCCACGCAG ACCCTACAAG CTTTTACTGG CTACACTGAC AAATTTGTAC 
CAGCAGCTGG ACCAGACTTA CACTGGACCA GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACAGACTACG AGGCAGTGTG TGGCCGTAGG GCACGCCTGG AGCGGGAAGT CCAGGGAGCC 
TTCCTGCGCT TCATGGCCTG TCTGCTCAAA GGCTACCGGG ACTTTCTGCG CCCCCTCACC 
CAGGCCCCCT CTGAGGGCTC TCGTGATGTT GACAACCTTT TCTACCTTCA GGGTTTCCTC 
AAGTCCCGGG AACGCTCAAG CCACAAACTG TACTCTCAGC TGCTGCACAC GCAGATGTTT 
TCACAGTTCA TTGAGGAATG CTCTTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TTGACAAGGT CCACCCAGAG CAGGAAAAGC CGGAGCCAAC ACCCTTAGTG 
GAGCTGGAGG AGCTGTCAGG AAGTGAGCTC ACTGTCTTCA TCACACCCCC AGAGGAGCCT 
CCAGTACTGG AGGGCAGTGA ATCTATACCA CAGTACTGTT ACGATGGGTT CCCAGAGCTA 
AGAGCTGAGC TATTTGAGTC TCCTCAAGAA CAACAGGGGG CCTTGCCTGT GCCAGGCCCA 
TCTCGTAGTG CCCCTAGCAG TCCTGCCCCT CGCCGTACCA AACAGGAGAT GAAGGTTGCA 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTTCTGGGG 
CACTGCTATG GACTGTGGTT CCTCTGCCTG CCTGCCTATG TGCGTTCAGC ACCCTCTCGG 
GTACGAGCAC TGCACACAGC CTACCATGTT CTGCGTGAGA TGGAGAGCCG CAAAGTGGTA 
CTCCCCGATG AGGTGTGTTA CCGAGTACTG ATGCAGCTCT GCTCACACTA TGGGCAGCCT 
GTGTTGTCTG TTCGAGTCAT GTTGGAGATG CGGCGGGCTG GCATCGTGCC CAACACAATC 
ACTTATGGTT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG TACACCAGGT 
GGGCGCCTAC GCTGGGCCAA GCTCCGGAAT GTTGTCCTCG GAGCTGCTCA GTTCCGCCAG 
CCCTTGAAAG AACGTTGGCA GCAGCAGCAG CGGCAGCAGC AACAGCAGCG GCAGCGGCGG 
CGGCAGCAGC AGCAGCAACA GATGGCCACG CACCAAGAAA GAGGCAGCTC TCAGACAGAG 
TCCTATCTGG AGCGCCCCTC CCCAACCCGC CCTCTTCAGC GCCAGACTAC CTGGGCTGGC 
CGAAGTCTTC GGGAACCAGC CTCACCTGCT GGGCGCCTGG TCAAAAGTGG CAGCCTAGGC 
AGTGCCCGAG GGGCACAGCC TACCGTGGAG GCTGGTGTGG CTCACATGAT AGAAGCCTTG 
GGGGTCCTAG AACCCCGTGG ATCACCTGTG CCTTGGCAAG ATGGAAGCCT TTCAGACCTG 
AGCCTGACAG GGGAAGAGCT GGCATCTGGG GGCAGTCCAG GGGGCTCAGG CTCGGCCCTG 
AGTGCCCAGT CCACTGAGGC CCTAGAAGGG CTAAGTGGGA GAGGTCCCAA AACTGGAGGA 
TGGCAGGAGG AGGCAGGCAC CCCCAGAAGA GGACTGGGTG CCCGCCTTCA ACAGCTGCTC 
ACTCCTTCCC GCCGCTCCTC TGCCTCCCGC ATCCCCCCAC CAGAGTTGTC CCCTGACCTT 
CCTCCTGTAG CCCGTCGCAG TCCCATGGAT AGCCTTCTGT GGCCCCGAGA ACGCCCTGGA 
TCCACTGCTT CTGAGAGCTC AGCCTCACTG GGCAGTGAGT GGGATCTCTC AGAGTCTTCT 
CTCAGCAGTC TGAGTCTCCG CCATTCCTCA GAGCGCCTCA GTGACACTCC TGGACCCTTT 
CAGCCACCTT CCCTGGAAAT TCTGCTGTCT AGCTGTTCCT TGTGTCGTGC CTGTGATTCA 
CTGGTGTATG ATGAGGAAAT CATGGCTGGC TGGGCACCTG ATGACTCCAA CCTCAACACA 
ATTTGCCCCT TCTGCGCCTG CCCGTTTGTG CCGCTCCTCA GTGTCCAGAC CCTTGATTCC 
CGACCTAGTG CCCCTAGCCC TAAGTCTACC CTTGCCAGTG CCAGTGGTAG CAAAGATGCT 
CCTGTCCCTG GGGGTCCTGG CCCTGTGCTC AGTGACCGAA GGCTCTGTCT TGCCTTGGAT 
GAACCCCAGC TCTGCAATGG ACATTTGGGG GGTGTCTCCC GGCGGGTTGA GAGTGGGGCA 
TGGGCATACC TAAGCCCCCT GGTGCTGCGT AAGGAGCTAG AGTCGCTGGT AGAGAATGAA 
GGCAGCGAGG TGTTGGCATT GCCTGAACTG CCTGCTGCCC ACCCCATCAT TTTCTGGAAC 
CTTCTGTGGT ATTTCCAACG GCTACGCCTG CCTAGTATTC TACCAGGCCT AGTGCTGGCC 
TCTTATGATG GGCCCCCACC CAACCAGCTC TCCCAGGCAC CATCTCCTTG GTTAACCCCT 
GATCCAGCCT CTGTGCATGT GCGGCTGTTG TGGGATGTCT TGACCCCTGA TCCTAACAGC 
TGCCCACCTC TCTACGTGCT CTGGAGGGTC CACAGCCAGA TCCCTCAACG GGTGGTATGG 
CCAGGCCCAG TACCTTCATG TCTTAGCTTG GCATTATTGG AGTCTGTGCT GCGTCATGTT 
GGACTTAATG AGGTGCACAA GGCTGTGGGG CTTCTGCTGG AAACTCTAGG ACCCCCTCCC 
ACTGGCCTGC ACCTGCAGAG GAGCATCTAT CGTGAGATCT TATTCCTGAC AATGGCAGCT 
CTGGGCAAGG ATCATGTGGA CATAGTGGCC TTCGACAAGA AGTACAAGTC TGCCTTTAAC 
AAGCTGGCCA GCAGCATGGG CAAGGAGGAA CTAAGGCAAC GACGGGCACA GATGCCTACT 
CCCAAGGCGA TTGACTGCCG AAAATGTTTT GGAGCACCTC TGGAATGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_017795122.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017650611.1 Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X2, mRNA.

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CloneID	ONa43210
Clone ID Related Accession (Same CDS sequence)	XM_017795122.2 , XM_017795122.1
Accession Version	XM_017795122.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008330926.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCCGTGAG TGAAGGGGGG GCTATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TTGTGGTAGC TGGGCTCGCT GGAAACGGAG CTCCTATACC TGAGGAAACA 
TGGGTTCCTG AACCCACTGG GCCCCTGCGC CCTCCCAGGC CAGCAGAGCC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAAGTGCCCC AGGGTTACAC GTGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGTAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGACCCAA GCTTGGTTTT CAAGTGCTCG ACACGACACC ATATAGCCAC 
TCGGCCAACC TGGCCCCTCC AGGCCCTGGA CACCCCCGCA CCTACCTCAT GTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGTG AAGGCACTCC TCATACTTAC TGCCGGTTGC CCCGCAATCT TAACCCTGGC 
ATGTGGGGCC CAGCAGTATA CCTGTGCTAC AAGGTGGGCC TGGCTAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC ATTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCGGTGCC TGTCTTCTCC ACCTTTGTGC TCACGGGTGC TGCTGGTGAT 
AAGGTGTATG GTGCCGCCCT GCAGTTCTAC GAAGCGTTCC CTAGGGCCAG GCTGTCAGAG 
AGGCAGGCAC GGGCACTGGG CCTGCTGAGT GCTGTGGAAC GGGGCCGGGC ACTGGGGGGC 
CGAGCTGTGC GCAGCCGGCG AGCCATTGCT GTACTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGAGCCT TCCTTACCTT CCTTTACCGC TACTCGGTCT CAGGCCCTCA TCGCCTGCCC 
TTGGAAGCGC ACATCTCACA CTTCATTCAC AACGTCCCAT TCCCTTCTCC ACAGAGACCA 
CGCATCCTAG TGCAGATGTC TCCCTATGAC AACCTGCTTC TCTGTCAACC TGTGTCCTCA 
CCCCTGCCCC TCAGTGGTGC TAGCTTTTTG CAGCTGCTGC AGAGCCTGGG CCCTGAGCTG 
GCTATTACAT TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTGGTCCA CTCACTGCGA 
CCAGACCTGC TCACCAGCGT CTGTGAGGCC CTTGTCTCAA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCGCTG GTGCTGGCGG ATGTGCTGAG TGCCCCAGTG 
CCCTTCATTG TGGGTATCCA CTCCAGCTAT TTTGATCTGC ACGACCCACC TGCTGATGTC 
ATCTGTGTTG ATCTTGACAC CAACACACTT TTCCAGACAG AAGAAAAGAA GCCCCTCTCC 
GCTAGGACCC TGCCACGCAG ACCCTACAAG CTTTTACTGG CTACACTGAC AAATTTGTAC 
CAGCAGCTGG ACCAGACTTA CACTGGACCA GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACAGACTACG AGGCAGTGTG TGGCCGTAGG GCACGCCTGG AGCGGGAAGT CCAGGGAGCC 
TTCCTGCGCT TCATGGCCTG TCTGCTCAAA GGCTACCGGG ACTTTCTGCG CCCCCTCACC 
CAGGCCCCCT CTGAGGGCTC TCGTGATGTT GACAACCTTT TCTACCTTCA GGGTTTCCTC 
AAGTCCCGGG AACGCTCAAG CCACAAACTG TACTCTCAGC TGCTGCACAC GCAGATGTTT 
TCACAGTTCA TTGAGGAATG CTCTTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TTGACAAGGT CCACCCAGAG CAGGAAAAGC CGGAGCCAAC ACCCTTAGTG 
GAGCTGGAGG AGCTGTCAGG AAGTGAGCTC ACTGTCTTCA TCACACCCCC AGAGGAGCCT 
CCAGTACTGG AGGGCAGTGA ATCTATACCA CAGTACTGTT ACGATGGGTT CCCAGAGCTA 
AGAGCTGAGC TATTTGAGTC TCCTCAAGAA CAACAGGGGG CCTTGCCTGT GCCAGGCCCA 
TCTCGTAGTG CCCCTAGCAG TCCTGCCCCT CGCCGTACCA AACAGGAGAT GAAGGTTGCA 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTTCTGGGG 
CACTGCTATG GACTGTGGTT CCTCTGCCTG CCTGCCTATG TGCGTTCAGC ACCCTCTCGG 
GTACGAGCAC TGCACACAGC CTACCATGTT CTGCGTGAGA TGGAGAGCCG CAAAGTGGTA 
CTCCCCGATG AGGTGTGTTA CCGAGTACTG ATGCAGCTCT GCTCACACTA TGGGCAGCCT 
GTGTTGTCTG TTCGAGTCAT GTTGGAGATG CGGCGGGCTG GCATCGTGCC CAACACAATC 
ACTTATGGTT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG TACACCAGGT 
GGGCGCCTAC GCTGGGCCAA GCTCCGGAAT GTTGTCCTCG GAGCTGCTCA GTTCCGCCAG 
CCCTTGAAAG AACGTTGGCA GCAGCAGCAG CGGCAGCAGC AACAGCAGCG GCAGCGGCGG 
CGGCAGCAGC AGCAGCAACA GATGGCCACG CACCAAGAAA GAGGCAGCTC TCAGACAGAG 
TCCTATCTGG AGCGCCCCTC CCCAACCCGC CCTCTTCAGC GCCAGACTAC CTGGGCTGGC 
CGAAGTCTTC GGGAACCAGC CTCACCTGCT GGGCGCCTGG TCAAAAGTGG CAGCCTAGGC 
AGTGCCCGAG GGGCACAGCC TACCGTGGAG GCTGGTGTGG CTCACATGAT AGAAGCCTTG 
GGGGTCCTAG AACCCCGTGG ATCACCTGTG CCTTGGCAAG ATGGAAGCCT TTCAGACCTG 
AGCCTGACAG GGGAAGAGCT GGCATCTGGG GGCAGTCCAG GGGGCTCAGG CTCGGCCCTG 
AGTGCCCAGT CCACTGAGGC CCTAGAAGGG CTAAGTGGGA GAGGTCCCAA AACTGGAGGA 
TGGCAGGAGG AGGCAGGCAC CCCCAGAAGA GGACTGGGTG CCCGCCTTCA ACAGCTGCTC 
ACTCCTTCCC GCCGCTCCTC TGCCTCCCGC ATCCCCCCAC CAGAGTTGTC CCCTGACCTT 
CCTCCTGTAG CCCGTCGCAG TCCCATGGAT AGCCTTCTGT GGCCCCGAGA ACGCCCTGGA 
TCCACTGCTT CTGAGAGCTC AGCCTCACTG GGCAGTGAGT GGGATCTCTC AGAGTCTTCT 
CTCAGCAGTC TGAGTCTCCG CCATTCCTCA GAGCGCCTCA GTGACACTCC TGGACCCTTT 
CAGCCACCTT CCCTGGAAAT TCTGCTGTCT AGCTGTTCCT TGTGTCGTGC CTGTGATTCA 
CTGGTGTATG ATGAGGAAAT CATGGCTGGC TGGGCACCTG ATGACTCCAA CCTCAACACA 
ATTTGCCCCT TCTGCGCCTG CCCGTTTGTG CCGCTCCTCA GTGTCCAGAC CCTTGATTCC 
CGACCTAGTG CCCCTAGCCC TAAGTCTACC CTTGCCAGTG CCAGTGGTAG CAAAGATGCT 
CCTGTCCCTG GGGGTCCTGG CCCTGTGCTC AGTGACCGAA GGCTCTGTCT TGCCTTGGAT 
GAACCCCAGC TCTGCAATGG ACATTTGGGG GGTGTCTCCC GGCGGGTTGA GAGTGGGGCA 
TGGGCATACC TAAGCCCCCT GGTGCTGCGT AAGGAGCTAG AGTCGCTGGT AGAGAATGAA 
GGCAGCGAGG TGTTGGCATT GCCTGAACTG CCTGCTGCCC ACCCCATCAT TTTCTGGAAC 
CTTCTGTGGT ATTTCCAACG GCTACGCCTG CCTAGTATTC TACCAGGCCT AGTGCTGGCC 
TCTTATGATG GGCCCCCACC CAACCAGCTC TCCCAGGCAC CATCTCCTTG GTTAACCCCT 
GATCCAGCCT CTGTGCATGT GCGGCTGTTG TGGGATGTCT TGACCCCTGA TCCTAACAGC 
TGCCCACCTC TCTACGTGCT CTGGAGGGTC CACAGCCAGA TCCCTCAACG GGTGGTATGG 
CCAGGCCCAG TACCTTCATG TCTTAGCTTG GCATTATTGG AGTCTGTGCT GCGTCATGTT 
GGACTTAATG AGGTGCACAA GGCTGTGGGG CTTCTGCTGG AAACTCTAGG ACCCCCTCCC 
ACTGGCCTGC ACCTGCAGAG GAGCATCTAT CGTGAGATCT TATTCCTGAC AATGGCAGCT 
CTGGGCAAGG ATCATGTGGA CATAGTGGCC TTCGACAAGA AGTACAAGTC TGCCTTTAAC 
AAGCTGGCCA GCAGCATGGG CAAGGAGGAA CTAAGGCAAC GACGGGCACA GATGCCTACT 
CCCAAGGCGA TTGACTGCCG AAAATGTTTT GGAGCACCTC TGGAATGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017650611.1
CDS	368..4918
Translation	MEADAVSEGGAMAEERPPRLVDYFVVAGLAGNGAPIPEETWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKLGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSARTLPRRPYKLLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESIPQYCYDGFPELRAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYHVLREMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKERWQQQQRQQQQQRQRRRQQQQQQMATHQERGSSQTESYLERPSPTRPLQRQTTWAGRSLREPASPAGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEELASGGSPGGSGSALSAQSTEALEGLSGRGPKTGGWQEEAGTPRRGLGARLQQLLTPSRRSSASRIPPPELSPDLPPVARRSPMDSLLWPRERPGSTASESSASLGSEWDLSESSLSSLSLRHSSERLSDTPGPFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTICPFCACPFVPLLSVQTLDSRPSAPSPKSTLASASGSKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHLGGVSRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPGLVLASYDGPPPNQLSQAPSPWLTPDPASVHVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRSIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_017795122.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017795122.1

ORF Insert Sequence:

ATGGAGGCAG ATGCCGTGAG TGAAGGGGGG GCTATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TTGTGGTAGC TGGGCTCGCT GGAAACGGAG CTCCTATACC TGAGGAAACA 
TGGGTTCCTG AACCCACTGG GCCCCTGCGC CCTCCCAGGC CAGCAGAGCC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAAGTGCCCC AGGGTTACAC GTGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGTAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGACCCAA GCTTGGTTTT CAAGTGCTCG ACACGACACC ATATAGCCAC 
TCGGCCAACC TGGCCCCTCC AGGCCCTGGA CACCCCCGCA CCTACCTCAT GTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGTG AAGGCACTCC TCATACTTAC TGCCGGTTGC CCCGCAATCT TAACCCTGGC 
ATGTGGGGCC CAGCAGTATA CCTGTGCTAC AAGGTGGGCC TGGCTAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC ATTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCGGTGCC TGTCTTCTCC ACCTTTGTGC TCACGGGTGC TGCTGGTGAT 
AAGGTGTATG GTGCCGCCCT GCAGTTCTAC GAAGCGTTCC CTAGGGCCAG GCTGTCAGAG 
AGGCAGGCAC GGGCACTGGG CCTGCTGAGT GCTGTGGAAC GGGGCCGGGC ACTGGGGGGC 
CGAGCTGTGC GCAGCCGGCG AGCCATTGCT GTACTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGAGCCT TCCTTACCTT CCTTTACCGC TACTCGGTCT CAGGCCCTCA TCGCCTGCCC 
TTGGAAGCGC ACATCTCACA CTTCATTCAC AACGTCCCAT TCCCTTCTCC ACAGAGACCA 
CGCATCCTAG TGCAGATGTC TCCCTATGAC AACCTGCTTC TCTGTCAACC TGTGTCCTCA 
CCCCTGCCCC TCAGTGGTGC TAGCTTTTTG CAGCTGCTGC AGAGCCTGGG CCCTGAGCTG 
GCTATTACAT TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTGGTCCA CTCACTGCGA 
CCAGACCTGC TCACCAGCGT CTGTGAGGCC CTTGTCTCAA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCGCTG GTGCTGGCGG ATGTGCTGAG TGCCCCAGTG 
CCCTTCATTG TGGGTATCCA CTCCAGCTAT TTTGATCTGC ACGACCCACC TGCTGATGTC 
ATCTGTGTTG ATCTTGACAC CAACACACTT TTCCAGACAG AAGAAAAGAA GCCCCTCTCC 
GCTAGGACCC TGCCACGCAG ACCCTACAAG CTTTTACTGG CTACACTGAC AAATTTGTAC 
CAGCAGCTGG ACCAGACTTA CACTGGACCA GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACAGACTACG AGGCAGTGTG TGGCCGTAGG GCACGCCTGG AGCGGGAAGT CCAGGGAGCC 
TTCCTGCGCT TCATGGCCTG TCTGCTCAAA GGCTACCGGG ACTTTCTGCG CCCCCTCACC 
CAGGCCCCCT CTGAGGGCTC TCGTGATGTT GACAACCTTT TCTACCTTCA GGGTTTCCTC 
AAGTCCCGGG AACGCTCAAG CCACAAACTG TACTCTCAGC TGCTGCACAC GCAGATGTTT 
TCACAGTTCA TTGAGGAATG CTCTTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TTGACAAGGT CCACCCAGAG CAGGAAAAGC CGGAGCCAAC ACCCTTAGTG 
GAGCTGGAGG AGCTGTCAGG AAGTGAGCTC ACTGTCTTCA TCACACCCCC AGAGGAGCCT 
CCAGTACTGG AGGGCAGTGA ATCTATACCA CAGTACTGTT ACGATGGGTT CCCAGAGCTA 
AGAGCTGAGC TATTTGAGTC TCCTCAAGAA CAACAGGGGG CCTTGCCTGT GCCAGGCCCA 
TCTCGTAGTG CCCCTAGCAG TCCTGCCCCT CGCCGTACCA AACAGGAGAT GAAGGTTGCA 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTTCTGGGG 
CACTGCTATG GACTGTGGTT CCTCTGCCTG CCTGCCTATG TGCGTTCAGC ACCCTCTCGG 
GTACGAGCAC TGCACACAGC CTACCATGTT CTGCGTGAGA TGGAGAGCCG CAAAGTGGTA 
CTCCCCGATG AGGTGTGTTA CCGAGTACTG ATGCAGCTCT GCTCACACTA TGGGCAGCCT 
GTGTTGTCTG TTCGAGTCAT GTTGGAGATG CGGCGGGCTG GCATCGTGCC CAACACAATC 
ACTTATGGTT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG TACACCAGGT 
GGGCGCCTAC GCTGGGCCAA GCTCCGGAAT GTTGTCCTCG GAGCTGCTCA GTTCCGCCAG 
CCCTTGAAAG AACGTTGGCA GCAGCAGCAG CGGCAGCAGC AACAGCAGCG GCAGCGGCGG 
CGGCAGCAGC AGCAGCAACA GATGGCCACG CACCAAGAAA GAGGCAGCTC TCAGACAGAG 
TCCTATCTGG AGCGCCCCTC CCCAACCCGC CCTCTTCAGC GCCAGACTAC CTGGGCTGGC 
CGAAGTCTTC GGGAACCAGC CTCACCTGCT GGGCGCCTGG TCAAAAGTGG CAGCCTAGGC 
AGTGCCCGAG GGGCACAGCC TACCGTGGAG GCTGGTGTGG CTCACATGAT AGAAGCCTTG 
GGGGTCCTAG AACCCCGTGG ATCACCTGTG CCTTGGCAAG ATGGAAGCCT TTCAGACCTG 
AGCCTGACAG GGGAAGAGCT GGCATCTGGG GGCAGTCCAG GGGGCTCAGG CTCGGCCCTG 
AGTGCCCAGT CCACTGAGGC CCTAGAAGGG CTAAGTGGGA GAGGTCCCAA AACTGGAGGA 
TGGCAGGAGG AGGCAGGCAC CCCCAGAAGA GGACTGGGTG CCCGCCTTCA ACAGCTGCTC 
ACTCCTTCCC GCCGCTCCTC TGCCTCCCGC ATCCCCCCAC CAGAGTTGTC CCCTGACCTT 
CCTCCTGTAG CCCGTCGCAG TCCCATGGAT AGCCTTCTGT GGCCCCGAGA ACGCCCTGGA 
TCCACTGCTT CTGAGAGCTC AGCCTCACTG GGCAGTGAGT GGGATCTCTC AGAGTCTTCT 
CTCAGCAGTC TGAGTCTCCG CCATTCCTCA GAGCGCCTCA GTGACACTCC TGGACCCTTT 
CAGCCACCTT CCCTGGAAAT TCTGCTGTCT AGCTGTTCCT TGTGTCGTGC CTGTGATTCA 
CTGGTGTATG ATGAGGAAAT CATGGCTGGC TGGGCACCTG ATGACTCCAA CCTCAACACA 
ATTTGCCCCT TCTGCGCCTG CCCGTTTGTG CCGCTCCTCA GTGTCCAGAC CCTTGATTCC 
CGACCTAGTG CCCCTAGCCC TAAGTCTACC CTTGCCAGTG CCAGTGGTAG CAAAGATGCT 
CCTGTCCCTG GGGGTCCTGG CCCTGTGCTC AGTGACCGAA GGCTCTGTCT TGCCTTGGAT 
GAACCCCAGC TCTGCAATGG ACATTTGGGG GGTGTCTCCC GGCGGGTTGA GAGTGGGGCA 
TGGGCATACC TAAGCCCCCT GGTGCTGCGT AAGGAGCTAG AGTCGCTGGT AGAGAATGAA 
GGCAGCGAGG TGTTGGCATT GCCTGAACTG CCTGCTGCCC ACCCCATCAT TTTCTGGAAC 
CTTCTGTGGT ATTTCCAACG GCTACGCCTG CCTAGTATTC TACCAGGCCT AGTGCTGGCC 
TCTTATGATG GGCCCCCACC CAACCAGCTC TCCCAGGCAC CATCTCCTTG GTTAACCCCT 
GATCCAGCCT CTGTGCATGT GCGGCTGTTG TGGGATGTCT TGACCCCTGA TCCTAACAGC 
TGCCCACCTC TCTACGTGCT CTGGAGGGTC CACAGCCAGA TCCCTCAACG GGTGGTATGG 
CCAGGCCCAG TACCTTCATG TCTTAGCTTG GCATTATTGG AGTCTGTGCT GCGTCATGTT 
GGACTTAATG AGGTGCACAA GGCTGTGGGG CTTCTGCTGG AAACTCTAGG ACCCCCTCCC 
ACTGGCCTGC ACCTGCAGAG GAGCATCTAT CGTGAGATCT TATTCCTGAC AATGGCAGCT 
CTGGGCAAGG ATCATGTGGA CATAGTGGCC TTCGACAAGA AGTACAAGTC TGCCTTTAAC 
AAGCTGGCCA GCAGCATGGG CAAGGAGGAA CTAAGGCAAC GACGGGCACA GATGCCTACT 
CCCAAGGCGA TTGACTGCCG AAAATGTTTT GGAGCACCTC TGGAATGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_008855255.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008853477.1 Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ONa43211
Clone ID Related Accession (Same CDS sequence)	XM_008855255.2 , XM_008855255.3
Accession Version	XM_008855255.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	DENN domain-containing protein 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008330926.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008855255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCAGG TGTATGGTGC CGCCCTGCAG TTCTACGAAG CGTTCCCTAG GGCCAGGCTG 
TCAGAGAGGC AGGCACGGGC ACTGGGCCTG CTGAGTGCTG TGGAACGGGG CCGGGCACTG 
GGGGGCCGAG CTGTGCGCAG CCGGCGAGCC ATTGCTGTAC TGTCCCGCTG GCCTGCCTTC 
CCAGCCTTCC GAGCCTTCCT TACCTTCCTT TACCGCTACT CGGTCTCAGG CCCTCATCGC 
CTGCCCTTGG AAGCGCACAT CTCACACTTC ATTCACAACG TCCCATTCCC TTCTCCACAG 
AGACCACGCA TCCTAGTGCA GATGTCTCCC TATGACAACC TGCTTCTCTG TCAACCTGTG 
TCCTCACCCC TGCCCCTCAG TGGTGCTAGC TTTTTGCAGC TGCTGCAGAG CCTGGGCCCT 
GAGCTGGCTA TTACATTGCT GCTGGCTGTG CTCACAGAGC ACAAACTGCT GGTCCACTCA 
CTGCGACCAG ACCTGCTCAC CAGCGTCTGT GAGGCCCTTG TCTCAATGAT CTTCCCGCTG 
CACTGGCAGT GCCCCTACAT TCCGCTGTGC CCGCTGGTGC TGGCGGATGT GCTGAGTGCC 
CCAGTGCCCT TCATTGTGGG TATCCACTCC AGCTATTTTG ATCTGCACGA CCCACCTGCT 
GATGTCATCT GTGTTGATCT TGACACCAAC ACACTTTTCC AGACAGAAGA AAAGAAGCCC 
CTCTCCGCTA GGACCCTGCC ACGCAGACCC TACAAGCTTT TACTGGCTAC ACTGACAAAT 
TTGTACCAGC AGCTGGACCA GACTTACACT GGACCAGAGG AGGAGGCCTC CCTGGAATTC 
CTGCTGACAG ACTACGAGGC AGTGTGTGGC CGTAGGGCAC GCCTGGAGCG GGAAGTCCAG 
GGAGCCTTCC TGCGCTTCAT GGCCTGTCTG CTCAAAGGCT ACCGGGACTT TCTGCGCCCC 
CTCACCCAGG CCCCCTCTGA GGGCTCTCGT GATGTTGACA ACCTTTTCTA CCTTCAGGGT 
TTCCTCAAGT CCCGGGAACG CTCAAGCCAC AAACTGTACT CTCAGCTGCT GCACACGCAG 
ATGTTTTCAC AGTTCATTGA GGAATGCTCT TTTGGCTCTG CTCGGCATGC TGCCCTTGAA 
TTCTTTGACT CTTGTGTTGA CAAGGTCCAC CCAGAGCAGG AAAAGCCGGA GCCAACACCC 
TTAGTGGAGC TGGAGGAGCT GTCAGGAAGT GAGCTCACTG TCTTCATCAC ACCCCCAGAG 
GAGCCTCCAG TACTGGAGGG CAGTGAATCT ATACCACAGT ACTGTTACGA TGGGTTCCCA 
GAGCTAAGAG CTGAGCTATT TGAGTCTCCT CAAGAACAAC AGGGGGCCTT GCCTGTGCCA 
GGCCCATCTC GTAGTGCCCC TAGCAGTCCT GCCCCTCGCC GTACCAAACA GGAGATGAAG 
GTTGCACAGC GGATGGCACA GAAGTCAGCA GCTGTGCCTG AGCTGTGGGC CCGGTGCCTT 
CTGGGGCACT GCTATGGACT GTGGTTCCTC TGCCTGCCTG CCTATGTGCG TTCAGCACCC 
TCTCGGGTAC GAGCACTGCA CACAGCCTAC CATGTTCTGC GTGAGATGGA GAGCCGCAAA 
GTGGTACTCC CCGATGAGGT GTGTTACCGA GTACTGATGC AGCTCTGCTC ACACTATGGG 
CAGCCTGTGT TGTCTGTTCG AGTCATGTTG GAGATGCGGC GGGCTGGCAT CGTGCCCAAC 
ACAATCACTT ATGGTTACTA CAACAAGGCT GTGCTGGAAA GCAAGTGGCC GTCTGGTACA 
CCAGGTGGGC GCCTACGCTG GGCCAAGCTC CGGAATGTTG TCCTCGGAGC TGCTCAGTTC 
CGCCAGCCCT TGAAAGAACG TTGGCAGCAG CAGCAGCGGC AGCAGCAACA GCAGCGGCAG 
CGGCGGCGGC AGCAGCAGCA GCAACAGATG GCCACGCACC AAGAAAGAGG CAGCTCTCAG 
ACAGAGTCCT ATCTGGAGCG CCCCTCCCCA ACCCGCCCTC TTCAGCGCCA GACTACCTGG 
GCTGGCCGAA GTCTTCGGGA ACCAGCCTCA CCTGCTGGGC GCCTGGTCAA AAGTGGCAGC 
CTAGGCAGTG CCCGAGGGGC ACAGCCTACC GTGGAGGCTG GTGTGGCTCA CATGATAGAA 
GCCTTGGGGG TCCTAGAACC CCGTGGATCA CCTGTGCCTT GGCAAGATGG AAGCCTTTCA 
GACCTGAGCC TGACAGGGGA AGAGCTGGCA TCTGGGGGCA GTCCAGGGGG CTCAGGCTCG 
GCCCTGAGTG CCCAGTCCAC TGAGGCCCTA GAAGGGCTAA GTGGGAGAGG TCCCAAAACT 
GGAGGATGGC AGGAGGAGGC AGGCACCCCC AGAAGAGGAC TGGGTGCCCG CCTTCAACAG 
CTGCTCACTC CTTCCCGCCG CTCCTCTGCC TCCCGCATCC CCCCACCAGA GTTGTCCCCT 
GACCTTCCTC CTGTAGCCCG TCGCAGTCCC ATGGATAGCC TTCTGTGGCC CCGAGAACGC 
CCTGGATCCA CTGCTTCTGA GAGCTCAGCC TCACTGGGCA GTGAGTGGGA TCTCTCAGAG 
TCTTCTCTCA GCAGTCTGAG TCTCCGCCAT TCCTCAGAGC GCCTCAGTGA CACTCCTGGA 
CCCTTTCAGC CACCTTCCCT GGAAATTCTG CTGTCTAGCT GTTCCTTGTG TCGTGCCTGT 
GATTCACTGG TGTATGATGA GGAAATCATG GCTGGCTGGG CACCTGATGA CTCCAACCTC 
AACACAATTT GCCCCTTCTG CGCCTGCCCG TTTGTGCCGC TCCTCAGTGT CCAGACCCTT 
GATTCCCGAC CTAGTGCCCC TAGCCCTAAG TCTACCCTTG CCAGTGCCAG TGGTAGCAAA 
GATGCTCCTG TCCCTGGGGG TCCTGGCCCT GTGCTCAGTG ACCGAAGGCT CTGTCTTGCC 
TTGGATGAAC CCCAGCTCTG CAATGGACAT TTGGGGGGTG TCTCCCGGCG GGTTGAGAGT 
GGGGCATGGG CATACCTAAG CCCCCTGGTG CTGCGTAAGG AGCTAGAGTC GCTGGTAGAG 
AATGAAGGCA GCGAGGTGTT GGCATTGCCT GAACTGCCTG CTGCCCACCC CATCATTTTC 
TGGAACCTTC TGTGGTATTT CCAACGGCTA CGCCTGCCTA GTATTCTACC AGGCCTAGTG 
CTGGCCTCTT ATGATGGGCC CCCACCCAAC CAGCTCTCCC AGGCACCATC TCCTTGGTTA 
ACCCCTGATC CAGCCTCTGT GCATGTGCGG CTGTTGTGGG ATGTCTTGAC CCCTGATCCT 
AACAGCTGCC CACCTCTCTA CGTGCTCTGG AGGGTCCACA GCCAGATCCC TCAACGGGTG 
GTATGGCCAG GCCCAGTACC TTCATGTCTT AGCTTGGCAT TATTGGAGTC TGTGCTGCGT 
CATGTTGGAC TTAATGAGGT GCACAAGGCT GTGGGGCTTC TGCTGGAAAC TCTAGGACCC 
CCTCCCACTG GCCTGCACCT GCAGAGGAGC ATCTATCGTG AGATCTTATT CCTGACAATG 
GCAGCTCTGG GCAAGGATCA TGTGGACATA GTGGCCTTCG ACAAGAAGTA CAAGTCTGCC 
TTTAACAAGC TGGCCAGCAG CATGGGCAAG GAGGAACTAA GGCAACGACG GGCACAGATG 
CCTACTCCCA AGGCGATTGA CTGCCGAAAA TGTTTTGGAG CACCTCTGGA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008853477.1
CDS	155..3871
Translation	MRQVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSARTLPRRPYKLLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESIPQYCYDGFPELRAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYHVLREMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKERWQQQQRQQQQQRQRRRQQQQQQMATHQERGSSQTESYLERPSPTRPLQRQTTWAGRSLREPASPAGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEELASGGSPGGSGSALSAQSTEALEGLSGRGPKTGGWQEEAGTPRRGLGARLQQLLTPSRRSSASRIPPPELSPDLPPVARRSPMDSLLWPRERPGSTASESSASLGSEWDLSESSLSSLSLRHSSERLSDTPGPFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTICPFCACPFVPLLSVQTLDSRPSAPSPKSTLASASGSKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHLGGVSRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPGLVLASYDGPPPNQLSQAPSPWLTPDPASVHVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRSIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_008855255.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008855255.2

ORF Insert Sequence:

ATGAGGCAGG TGTATGGTGC CGCCCTGCAG TTCTACGAAG CGTTCCCTAG GGCCAGGCTG 
TCAGAGAGGC AGGCACGGGC ACTGGGCCTG CTGAGTGCTG TGGAACGGGG CCGGGCACTG 
GGGGGCCGAG CTGTGCGCAG CCGGCGAGCC ATTGCTGTAC TGTCCCGCTG GCCTGCCTTC 
CCAGCCTTCC GAGCCTTCCT TACCTTCCTT TACCGCTACT CGGTCTCAGG CCCTCATCGC 
CTGCCCTTGG AAGCGCACAT CTCACACTTC ATTCACAACG TCCCATTCCC TTCTCCACAG 
AGACCACGCA TCCTAGTGCA GATGTCTCCC TATGACAACC TGCTTCTCTG TCAACCTGTG 
TCCTCACCCC TGCCCCTCAG TGGTGCTAGC TTTTTGCAGC TGCTGCAGAG CCTGGGCCCT 
GAGCTGGCTA TTACATTGCT GCTGGCTGTG CTCACAGAGC ACAAACTGCT GGTCCACTCA 
CTGCGACCAG ACCTGCTCAC CAGCGTCTGT GAGGCCCTTG TCTCAATGAT CTTCCCGCTG 
CACTGGCAGT GCCCCTACAT TCCGCTGTGC CCGCTGGTGC TGGCGGATGT GCTGAGTGCC 
CCAGTGCCCT TCATTGTGGG TATCCACTCC AGCTATTTTG ATCTGCACGA CCCACCTGCT 
GATGTCATCT GTGTTGATCT TGACACCAAC ACACTTTTCC AGACAGAAGA AAAGAAGCCC 
CTCTCCGCTA GGACCCTGCC ACGCAGACCC TACAAGCTTT TACTGGCTAC ACTGACAAAT 
TTGTACCAGC AGCTGGACCA GACTTACACT GGACCAGAGG AGGAGGCCTC CCTGGAATTC 
CTGCTGACAG ACTACGAGGC AGTGTGTGGC CGTAGGGCAC GCCTGGAGCG GGAAGTCCAG 
GGAGCCTTCC TGCGCTTCAT GGCCTGTCTG CTCAAAGGCT ACCGGGACTT TCTGCGCCCC 
CTCACCCAGG CCCCCTCTGA GGGCTCTCGT GATGTTGACA ACCTTTTCTA CCTTCAGGGT 
TTCCTCAAGT CCCGGGAACG CTCAAGCCAC AAACTGTACT CTCAGCTGCT GCACACGCAG 
ATGTTTTCAC AGTTCATTGA GGAATGCTCT TTTGGCTCTG CTCGGCATGC TGCCCTTGAA 
TTCTTTGACT CTTGTGTTGA CAAGGTCCAC CCAGAGCAGG AAAAGCCGGA GCCAACACCC 
TTAGTGGAGC TGGAGGAGCT GTCAGGAAGT GAGCTCACTG TCTTCATCAC ACCCCCAGAG 
GAGCCTCCAG TACTGGAGGG CAGTGAATCT ATACCACAGT ACTGTTACGA TGGGTTCCCA 
GAGCTAAGAG CTGAGCTATT TGAGTCTCCT CAAGAACAAC AGGGGGCCTT GCCTGTGCCA 
GGCCCATCTC GTAGTGCCCC TAGCAGTCCT GCCCCTCGCC GTACCAAACA GGAGATGAAG 
GTTGCACAGC GGATGGCACA GAAGTCAGCA GCTGTGCCTG AGCTGTGGGC CCGGTGCCTT 
CTGGGGCACT GCTATGGACT GTGGTTCCTC TGCCTGCCTG CCTATGTGCG TTCAGCACCC 
TCTCGGGTAC GAGCACTGCA CACAGCCTAC CATGTTCTGC GTGAGATGGA GAGCCGCAAA 
GTGGTACTCC CCGATGAGGT GTGTTACCGA GTACTGATGC AGCTCTGCTC ACACTATGGG 
CAGCCTGTGT TGTCTGTTCG AGTCATGTTG GAGATGCGGC GGGCTGGCAT CGTGCCCAAC 
ACAATCACTT ATGGTTACTA CAACAAGGCT GTGCTGGAAA GCAAGTGGCC GTCTGGTACA 
CCAGGTGGGC GCCTACGCTG GGCCAAGCTC CGGAATGTTG TCCTCGGAGC TGCTCAGTTC 
CGCCAGCCCT TGAAAGAACG TTGGCAGCAG CAGCAGCGGC AGCAGCAACA GCAGCGGCAG 
CGGCGGCGGC AGCAGCAGCA GCAACAGATG GCCACGCACC AAGAAAGAGG CAGCTCTCAG 
ACAGAGTCCT ATCTGGAGCG CCCCTCCCCA ACCCGCCCTC TTCAGCGCCA GACTACCTGG 
GCTGGCCGAA GTCTTCGGGA ACCAGCCTCA CCTGCTGGGC GCCTGGTCAA AAGTGGCAGC 
CTAGGCAGTG CCCGAGGGGC ACAGCCTACC GTGGAGGCTG GTGTGGCTCA CATGATAGAA 
GCCTTGGGGG TCCTAGAACC CCGTGGATCA CCTGTGCCTT GGCAAGATGG AAGCCTTTCA 
GACCTGAGCC TGACAGGGGA AGAGCTGGCA TCTGGGGGCA GTCCAGGGGG CTCAGGCTCG 
GCCCTGAGTG CCCAGTCCAC TGAGGCCCTA GAAGGGCTAA GTGGGAGAGG TCCCAAAACT 
GGAGGATGGC AGGAGGAGGC AGGCACCCCC AGAAGAGGAC TGGGTGCCCG CCTTCAACAG 
CTGCTCACTC CTTCCCGCCG CTCCTCTGCC TCCCGCATCC CCCCACCAGA GTTGTCCCCT 
GACCTTCCTC CTGTAGCCCG TCGCAGTCCC ATGGATAGCC TTCTGTGGCC CCGAGAACGC 
CCTGGATCCA CTGCTTCTGA GAGCTCAGCC TCACTGGGCA GTGAGTGGGA TCTCTCAGAG 
TCTTCTCTCA GCAGTCTGAG TCTCCGCCAT TCCTCAGAGC GCCTCAGTGA CACTCCTGGA 
CCCTTTCAGC CACCTTCCCT GGAAATTCTG CTGTCTAGCT GTTCCTTGTG TCGTGCCTGT 
GATTCACTGG TGTATGATGA GGAAATCATG GCTGGCTGGG CACCTGATGA CTCCAACCTC 
AACACAATTT GCCCCTTCTG CGCCTGCCCG TTTGTGCCGC TCCTCAGTGT CCAGACCCTT 
GATTCCCGAC CTAGTGCCCC TAGCCCTAAG TCTACCCTTG CCAGTGCCAG TGGTAGCAAA 
GATGCTCCTG TCCCTGGGGG TCCTGGCCCT GTGCTCAGTG ACCGAAGGCT CTGTCTTGCC 
TTGGATGAAC CCCAGCTCTG CAATGGACAT TTGGGGGGTG TCTCCCGGCG GGTTGAGAGT 
GGGGCATGGG CATACCTAAG CCCCCTGGTG CTGCGTAAGG AGCTAGAGTC GCTGGTAGAG 
AATGAAGGCA GCGAGGTGTT GGCATTGCCT GAACTGCCTG CTGCCCACCC CATCATTTTC 
TGGAACCTTC TGTGGTATTT CCAACGGCTA CGCCTGCCTA GTATTCTACC AGGCCTAGTG 
CTGGCCTCTT ATGATGGGCC CCCACCCAAC CAGCTCTCCC AGGCACCATC TCCTTGGTTA 
ACCCCTGATC CAGCCTCTGT GCATGTGCGG CTGTTGTGGG ATGTCTTGAC CCCTGATCCT 
AACAGCTGCC CACCTCTCTA CGTGCTCTGG AGGGTCCACA GCCAGATCCC TCAACGGGTG 
GTATGGCCAG GCCCAGTACC TTCATGTCTT AGCTTGGCAT TATTGGAGTC TGTGCTGCGT 
CATGTTGGAC TTAATGAGGT GCACAAGGCT GTGGGGCTTC TGCTGGAAAC TCTAGGACCC 
CCTCCCACTG GCCTGCACCT GCAGAGGAGC ATCTATCGTG AGATCTTATT CCTGACAATG 
GCAGCTCTGG GCAAGGATCA TGTGGACATA GTGGCCTTCG ACAAGAAGTA CAAGTCTGCC 
TTTAACAAGC TGGCCAGCAG CATGGGCAAG GAGGAACTAA GGCAACGACG GGCACAGATG 
CCTACTCCCA AGGCGATTGA CTGCCGAAAA TGTTTTGGAG CACCTCTGGA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_008855255.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008853477.1 Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X4, mRNA.

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CloneID	ONa43211
Clone ID Related Accession (Same CDS sequence)	XM_008855255.2 , XM_008855255.3
Accession Version	XM_008855255.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	DENN domain-containing protein 4B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008330926.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008855255.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCAGG TGTATGGTGC CGCCCTGCAG TTCTACGAAG CGTTCCCTAG GGCCAGGCTG 
TCAGAGAGGC AGGCACGGGC ACTGGGCCTG CTGAGTGCTG TGGAACGGGG CCGGGCACTG 
GGGGGCCGAG CTGTGCGCAG CCGGCGAGCC ATTGCTGTAC TGTCCCGCTG GCCTGCCTTC 
CCAGCCTTCC GAGCCTTCCT TACCTTCCTT TACCGCTACT CGGTCTCAGG CCCTCATCGC 
CTGCCCTTGG AAGCGCACAT CTCACACTTC ATTCACAACG TCCCATTCCC TTCTCCACAG 
AGACCACGCA TCCTAGTGCA GATGTCTCCC TATGACAACC TGCTTCTCTG TCAACCTGTG 
TCCTCACCCC TGCCCCTCAG TGGTGCTAGC TTTTTGCAGC TGCTGCAGAG CCTGGGCCCT 
GAGCTGGCTA TTACATTGCT GCTGGCTGTG CTCACAGAGC ACAAACTGCT GGTCCACTCA 
CTGCGACCAG ACCTGCTCAC CAGCGTCTGT GAGGCCCTTG TCTCAATGAT CTTCCCGCTG 
CACTGGCAGT GCCCCTACAT TCCGCTGTGC CCGCTGGTGC TGGCGGATGT GCTGAGTGCC 
CCAGTGCCCT TCATTGTGGG TATCCACTCC AGCTATTTTG ATCTGCACGA CCCACCTGCT 
GATGTCATCT GTGTTGATCT TGACACCAAC ACACTTTTCC AGACAGAAGA AAAGAAGCCC 
CTCTCCGCTA GGACCCTGCC ACGCAGACCC TACAAGCTTT TACTGGCTAC ACTGACAAAT 
TTGTACCAGC AGCTGGACCA GACTTACACT GGACCAGAGG AGGAGGCCTC CCTGGAATTC 
CTGCTGACAG ACTACGAGGC AGTGTGTGGC CGTAGGGCAC GCCTGGAGCG GGAAGTCCAG 
GGAGCCTTCC TGCGCTTCAT GGCCTGTCTG CTCAAAGGCT ACCGGGACTT TCTGCGCCCC 
CTCACCCAGG CCCCCTCTGA GGGCTCTCGT GATGTTGACA ACCTTTTCTA CCTTCAGGGT 
TTCCTCAAGT CCCGGGAACG CTCAAGCCAC AAACTGTACT CTCAGCTGCT GCACACGCAG 
ATGTTTTCAC AGTTCATTGA GGAATGCTCT TTTGGCTCTG CTCGGCATGC TGCCCTTGAA 
TTCTTTGACT CTTGTGTTGA CAAGGTCCAC CCAGAGCAGG AAAAGCCGGA GCCAACACCC 
TTAGTGGAGC TGGAGGAGCT GTCAGGAAGT GAGCTCACTG TCTTCATCAC ACCCCCAGAG 
GAGCCTCCAG TACTGGAGGG CAGTGAATCT ATACCACAGT ACTGTTACGA TGGGTTCCCA 
GAGCTAAGAG CTGAGCTATT TGAGTCTCCT CAAGAACAAC AGGGGGCCTT GCCTGTGCCA 
GGCCCATCTC GTAGTGCCCC TAGCAGTCCT GCCCCTCGCC GTACCAAACA GGAGATGAAG 
GTTGCACAGC GGATGGCACA GAAGTCAGCA GCTGTGCCTG AGCTGTGGGC CCGGTGCCTT 
CTGGGGCACT GCTATGGACT GTGGTTCCTC TGCCTGCCTG CCTATGTGCG TTCAGCACCC 
TCTCGGGTAC GAGCACTGCA CACAGCCTAC CATGTTCTGC GTGAGATGGA GAGCCGCAAA 
GTGGTACTCC CCGATGAGGT GTGTTACCGA GTACTGATGC AGCTCTGCTC ACACTATGGG 
CAGCCTGTGT TGTCTGTTCG AGTCATGTTG GAGATGCGGC GGGCTGGCAT CGTGCCCAAC 
ACAATCACTT ATGGTTACTA CAACAAGGCT GTGCTGGAAA GCAAGTGGCC GTCTGGTACA 
CCAGGTGGGC GCCTACGCTG GGCCAAGCTC CGGAATGTTG TCCTCGGAGC TGCTCAGTTC 
CGCCAGCCCT TGAAAGAACG TTGGCAGCAG CAGCAGCGGC AGCAGCAACA GCAGCGGCAG 
CGGCGGCGGC AGCAGCAGCA GCAACAGATG GCCACGCACC AAGAAAGAGG CAGCTCTCAG 
ACAGAGTCCT ATCTGGAGCG CCCCTCCCCA ACCCGCCCTC TTCAGCGCCA GACTACCTGG 
GCTGGCCGAA GTCTTCGGGA ACCAGCCTCA CCTGCTGGGC GCCTGGTCAA AAGTGGCAGC 
CTAGGCAGTG CCCGAGGGGC ACAGCCTACC GTGGAGGCTG GTGTGGCTCA CATGATAGAA 
GCCTTGGGGG TCCTAGAACC CCGTGGATCA CCTGTGCCTT GGCAAGATGG AAGCCTTTCA 
GACCTGAGCC TGACAGGGGA AGAGCTGGCA TCTGGGGGCA GTCCAGGGGG CTCAGGCTCG 
GCCCTGAGTG CCCAGTCCAC TGAGGCCCTA GAAGGGCTAA GTGGGAGAGG TCCCAAAACT 
GGAGGATGGC AGGAGGAGGC AGGCACCCCC AGAAGAGGAC TGGGTGCCCG CCTTCAACAG 
CTGCTCACTC CTTCCCGCCG CTCCTCTGCC TCCCGCATCC CCCCACCAGA GTTGTCCCCT 
GACCTTCCTC CTGTAGCCCG TCGCAGTCCC ATGGATAGCC TTCTGTGGCC CCGAGAACGC 
CCTGGATCCA CTGCTTCTGA GAGCTCAGCC TCACTGGGCA GTGAGTGGGA TCTCTCAGAG 
TCTTCTCTCA GCAGTCTGAG TCTCCGCCAT TCCTCAGAGC GCCTCAGTGA CACTCCTGGA 
CCCTTTCAGC CACCTTCCCT GGAAATTCTG CTGTCTAGCT GTTCCTTGTG TCGTGCCTGT 
GATTCACTGG TGTATGATGA GGAAATCATG GCTGGCTGGG CACCTGATGA CTCCAACCTC 
AACACAATTT GCCCCTTCTG CGCCTGCCCG TTTGTGCCGC TCCTCAGTGT CCAGACCCTT 
GATTCCCGAC CTAGTGCCCC TAGCCCTAAG TCTACCCTTG CCAGTGCCAG TGGTAGCAAA 
GATGCTCCTG TCCCTGGGGG TCCTGGCCCT GTGCTCAGTG ACCGAAGGCT CTGTCTTGCC 
TTGGATGAAC CCCAGCTCTG CAATGGACAT TTGGGGGGTG TCTCCCGGCG GGTTGAGAGT 
GGGGCATGGG CATACCTAAG CCCCCTGGTG CTGCGTAAGG AGCTAGAGTC GCTGGTAGAG 
AATGAAGGCA GCGAGGTGTT GGCATTGCCT GAACTGCCTG CTGCCCACCC CATCATTTTC 
TGGAACCTTC TGTGGTATTT CCAACGGCTA CGCCTGCCTA GTATTCTACC AGGCCTAGTG 
CTGGCCTCTT ATGATGGGCC CCCACCCAAC CAGCTCTCCC AGGCACCATC TCCTTGGTTA 
ACCCCTGATC CAGCCTCTGT GCATGTGCGG CTGTTGTGGG ATGTCTTGAC CCCTGATCCT 
AACAGCTGCC CACCTCTCTA CGTGCTCTGG AGGGTCCACA GCCAGATCCC TCAACGGGTG 
GTATGGCCAG GCCCAGTACC TTCATGTCTT AGCTTGGCAT TATTGGAGTC TGTGCTGCGT 
CATGTTGGAC TTAATGAGGT GCACAAGGCT GTGGGGCTTC TGCTGGAAAC TCTAGGACCC 
CCTCCCACTG GCCTGCACCT GCAGAGGAGC ATCTATCGTG AGATCTTATT CCTGACAATG 
GCAGCTCTGG GCAAGGATCA TGTGGACATA GTGGCCTTCG ACAAGAAGTA CAAGTCTGCC 
TTTAACAAGC TGGCCAGCAG CATGGGCAAG GAGGAACTAA GGCAACGACG GGCACAGATG 
CCTACTCCCA AGGCGATTGA CTGCCGAAAA TGTTTTGGAG CACCTCTGGA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008853477.1
CDS	120..3836
Translation	MRQVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSARTLPRRPYKLLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESIPQYCYDGFPELRAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYHVLREMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKERWQQQQRQQQQQRQRRRQQQQQQMATHQERGSSQTESYLERPSPTRPLQRQTTWAGRSLREPASPAGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEELASGGSPGGSGSALSAQSTEALEGLSGRGPKTGGWQEEAGTPRRGLGARLQQLLTPSRRSSASRIPPPELSPDLPPVARRSPMDSLLWPRERPGSTASESSASLGSEWDLSESSLSSLSLRHSSERLSDTPGPFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTICPFCACPFVPLLSVQTLDSRPSAPSPKSTLASASGSKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHLGGVSRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPGLVLASYDGPPPNQLSQAPSPWLTPDPASVHVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRSIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_008855255.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili DENN domain containing 4B (Dennd4b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008855255.3

ORF Insert Sequence:

ATGAGGCAGG TGTATGGTGC CGCCCTGCAG TTCTACGAAG CGTTCCCTAG GGCCAGGCTG 
TCAGAGAGGC AGGCACGGGC ACTGGGCCTG CTGAGTGCTG TGGAACGGGG CCGGGCACTG 
GGGGGCCGAG CTGTGCGCAG CCGGCGAGCC ATTGCTGTAC TGTCCCGCTG GCCTGCCTTC 
CCAGCCTTCC GAGCCTTCCT TACCTTCCTT TACCGCTACT CGGTCTCAGG CCCTCATCGC 
CTGCCCTTGG AAGCGCACAT CTCACACTTC ATTCACAACG TCCCATTCCC TTCTCCACAG 
AGACCACGCA TCCTAGTGCA GATGTCTCCC TATGACAACC TGCTTCTCTG TCAACCTGTG 
TCCTCACCCC TGCCCCTCAG TGGTGCTAGC TTTTTGCAGC TGCTGCAGAG CCTGGGCCCT 
GAGCTGGCTA TTACATTGCT GCTGGCTGTG CTCACAGAGC ACAAACTGCT GGTCCACTCA 
CTGCGACCAG ACCTGCTCAC CAGCGTCTGT GAGGCCCTTG TCTCAATGAT CTTCCCGCTG 
CACTGGCAGT GCCCCTACAT TCCGCTGTGC CCGCTGGTGC TGGCGGATGT GCTGAGTGCC 
CCAGTGCCCT TCATTGTGGG TATCCACTCC AGCTATTTTG ATCTGCACGA CCCACCTGCT 
GATGTCATCT GTGTTGATCT TGACACCAAC ACACTTTTCC AGACAGAAGA AAAGAAGCCC 
CTCTCCGCTA GGACCCTGCC ACGCAGACCC TACAAGCTTT TACTGGCTAC ACTGACAAAT 
TTGTACCAGC AGCTGGACCA GACTTACACT GGACCAGAGG AGGAGGCCTC CCTGGAATTC 
CTGCTGACAG ACTACGAGGC AGTGTGTGGC CGTAGGGCAC GCCTGGAGCG GGAAGTCCAG 
GGAGCCTTCC TGCGCTTCAT GGCCTGTCTG CTCAAAGGCT ACCGGGACTT TCTGCGCCCC 
CTCACCCAGG CCCCCTCTGA GGGCTCTCGT GATGTTGACA ACCTTTTCTA CCTTCAGGGT 
TTCCTCAAGT CCCGGGAACG CTCAAGCCAC AAACTGTACT CTCAGCTGCT GCACACGCAG 
ATGTTTTCAC AGTTCATTGA GGAATGCTCT TTTGGCTCTG CTCGGCATGC TGCCCTTGAA 
TTCTTTGACT CTTGTGTTGA CAAGGTCCAC CCAGAGCAGG AAAAGCCGGA GCCAACACCC 
TTAGTGGAGC TGGAGGAGCT GTCAGGAAGT GAGCTCACTG TCTTCATCAC ACCCCCAGAG 
GAGCCTCCAG TACTGGAGGG CAGTGAATCT ATACCACAGT ACTGTTACGA TGGGTTCCCA 
GAGCTAAGAG CTGAGCTATT TGAGTCTCCT CAAGAACAAC AGGGGGCCTT GCCTGTGCCA 
GGCCCATCTC GTAGTGCCCC TAGCAGTCCT GCCCCTCGCC GTACCAAACA GGAGATGAAG 
GTTGCACAGC GGATGGCACA GAAGTCAGCA GCTGTGCCTG AGCTGTGGGC CCGGTGCCTT 
CTGGGGCACT GCTATGGACT GTGGTTCCTC TGCCTGCCTG CCTATGTGCG TTCAGCACCC 
TCTCGGGTAC GAGCACTGCA CACAGCCTAC CATGTTCTGC GTGAGATGGA GAGCCGCAAA 
GTGGTACTCC CCGATGAGGT GTGTTACCGA GTACTGATGC AGCTCTGCTC ACACTATGGG 
CAGCCTGTGT TGTCTGTTCG AGTCATGTTG GAGATGCGGC GGGCTGGCAT CGTGCCCAAC 
ACAATCACTT ATGGTTACTA CAACAAGGCT GTGCTGGAAA GCAAGTGGCC GTCTGGTACA 
CCAGGTGGGC GCCTACGCTG GGCCAAGCTC CGGAATGTTG TCCTCGGAGC TGCTCAGTTC 
CGCCAGCCCT TGAAAGAACG TTGGCAGCAG CAGCAGCGGC AGCAGCAACA GCAGCGGCAG 
CGGCGGCGGC AGCAGCAGCA GCAACAGATG GCCACGCACC AAGAAAGAGG CAGCTCTCAG 
ACAGAGTCCT ATCTGGAGCG CCCCTCCCCA ACCCGCCCTC TTCAGCGCCA GACTACCTGG 
GCTGGCCGAA GTCTTCGGGA ACCAGCCTCA CCTGCTGGGC GCCTGGTCAA AAGTGGCAGC 
CTAGGCAGTG CCCGAGGGGC ACAGCCTACC GTGGAGGCTG GTGTGGCTCA CATGATAGAA 
GCCTTGGGGG TCCTAGAACC CCGTGGATCA CCTGTGCCTT GGCAAGATGG AAGCCTTTCA 
GACCTGAGCC TGACAGGGGA AGAGCTGGCA TCTGGGGGCA GTCCAGGGGG CTCAGGCTCG 
GCCCTGAGTG CCCAGTCCAC TGAGGCCCTA GAAGGGCTAA GTGGGAGAGG TCCCAAAACT 
GGAGGATGGC AGGAGGAGGC AGGCACCCCC AGAAGAGGAC TGGGTGCCCG CCTTCAACAG 
CTGCTCACTC CTTCCCGCCG CTCCTCTGCC TCCCGCATCC CCCCACCAGA GTTGTCCCCT 
GACCTTCCTC CTGTAGCCCG TCGCAGTCCC ATGGATAGCC TTCTGTGGCC CCGAGAACGC 
CCTGGATCCA CTGCTTCTGA GAGCTCAGCC TCACTGGGCA GTGAGTGGGA TCTCTCAGAG 
TCTTCTCTCA GCAGTCTGAG TCTCCGCCAT TCCTCAGAGC GCCTCAGTGA CACTCCTGGA 
CCCTTTCAGC CACCTTCCCT GGAAATTCTG CTGTCTAGCT GTTCCTTGTG TCGTGCCTGT 
GATTCACTGG TGTATGATGA GGAAATCATG GCTGGCTGGG CACCTGATGA CTCCAACCTC 
AACACAATTT GCCCCTTCTG CGCCTGCCCG TTTGTGCCGC TCCTCAGTGT CCAGACCCTT 
GATTCCCGAC CTAGTGCCCC TAGCCCTAAG TCTACCCTTG CCAGTGCCAG TGGTAGCAAA 
GATGCTCCTG TCCCTGGGGG TCCTGGCCCT GTGCTCAGTG ACCGAAGGCT CTGTCTTGCC 
TTGGATGAAC CCCAGCTCTG CAATGGACAT TTGGGGGGTG TCTCCCGGCG GGTTGAGAGT 
GGGGCATGGG CATACCTAAG CCCCCTGGTG CTGCGTAAGG AGCTAGAGTC GCTGGTAGAG 
AATGAAGGCA GCGAGGTGTT GGCATTGCCT GAACTGCCTG CTGCCCACCC CATCATTTTC 
TGGAACCTTC TGTGGTATTT CCAACGGCTA CGCCTGCCTA GTATTCTACC AGGCCTAGTG 
CTGGCCTCTT ATGATGGGCC CCCACCCAAC CAGCTCTCCC AGGCACCATC TCCTTGGTTA 
ACCCCTGATC CAGCCTCTGT GCATGTGCGG CTGTTGTGGG ATGTCTTGAC CCCTGATCCT 
AACAGCTGCC CACCTCTCTA CGTGCTCTGG AGGGTCCACA GCCAGATCCC TCAACGGGTG 
GTATGGCCAG GCCCAGTACC TTCATGTCTT AGCTTGGCAT TATTGGAGTC TGTGCTGCGT 
CATGTTGGAC TTAATGAGGT GCACAAGGCT GTGGGGCTTC TGCTGGAAAC TCTAGGACCC 
CCTCCCACTG GCCTGCACCT GCAGAGGAGC ATCTATCGTG AGATCTTATT CCTGACAATG 
GCAGCTCTGG GCAAGGATCA TGTGGACATA GTGGCCTTCG ACAAGAAGTA CAAGTCTGCC 
TTTAACAAGC TGGCCAGCAG CATGGGCAAG GAGGAACTAA GGCAACGACG GGCACAGATG 
CCTACTCCCA AGGCGATTGA CTGCCGAAAA TGTTTTGGAG CACCTCTGGA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Dennd4b cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム