Kcnma1 cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnma1
Entrez Gene ID	103747438
Full Name	potassium calcium-activated channel subfamily M alpha 1
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008849856.2	XP_008848078.1	calcium-activated potassium channel subunit alpha-1 isoform X4
XM_008849861.2	XP_008848083.1	calcium-activated potassium channel subunit alpha-1 isoform X9
XM_008849881.2	XP_008848103.1	calcium-activated potassium channel subunit alpha-1 isoform X25
XM_029554474.1	XP_029410334.1	calcium-activated potassium channel subunit alpha-1 isoform X6
XM_008849871.2	XP_008848093.1	calcium-activated potassium channel subunit alpha-1 isoform X11
XM_008849854.2	XP_008848076.1	calcium-activated potassium channel subunit alpha-1 isoform X2
XM_008849889.2	XP_008848111.1	calcium-activated potassium channel subunit alpha-1 isoform X31
XM_008849855.2	XP_008848077.1	calcium-activated potassium channel subunit alpha-1 isoform X1
XM_029554471.1	XP_029410331.1	calcium-activated potassium channel subunit alpha-1 isoform X3
XM_029554473.1	XP_029410333.1	calcium-activated potassium channel subunit alpha-1 isoform X5
XM_008849864.2	XP_008848086.1	calcium-activated potassium channel subunit alpha-1 isoform X8
XM_008849875.2	XP_008848097.1	calcium-activated potassium channel subunit alpha-1 isoform X14
XM_008849880.2	XP_008848102.1	calcium-activated potassium channel subunit alpha-1 isoform X16
XM_008849883.2	XP_008848105.1	calcium-activated potassium channel subunit alpha-1 isoform X19
XM_029554476.1	XP_029410336.1	calcium-activated potassium channel subunit alpha-1 isoform X21
XM_008849853.2	XP_008848075.1	calcium-activated potassium channel subunit alpha-1 isoform X1
XM_008849857.2	XP_008848079.1	calcium-activated potassium channel subunit alpha-1 isoform X5
XM_017802381.1	XP_017657870.1	calcium-activated potassium channel subunit alpha-1 isoform X8
XM_008849863.2	XP_008848085.1	calcium-activated potassium channel subunit alpha-1 isoform X10
XM_008849867.2	XP_008848089.1	calcium-activated potassium channel subunit alpha-1 isoform X14
XM_029554470.1	XP_029410330.1	calcium-activated potassium channel subunit alpha-1 isoform X2
XM_008849865.2	XP_008848087.1	calcium-activated potassium channel subunit alpha-1 isoform X9
XM_008849872.2	XP_008848094.1	calcium-activated potassium channel subunit alpha-1 isoform X12
XM_008849876.2	XP_008848098.1	calcium-activated potassium channel subunit alpha-1 isoform X15
XM_008849881.3	XP_008848103.1	calcium-activated potassium channel subunit alpha-1 isoform X17
XM_029554477.1	XP_029410337.1	calcium-activated potassium channel subunit alpha-1 isoform X22
XM_029554478.1	XP_029410338.1	calcium-activated potassium channel subunit alpha-1 isoform X23
XM_008849858.2	XP_008848080.1	calcium-activated potassium channel subunit alpha-1 isoform X6
XM_008849859.2	XP_008848081.1	calcium-activated potassium channel subunit alpha-1 isoform X7
XM_008849882.2	XP_008848104.1	calcium-activated potassium channel subunit alpha-1 isoform X26
XM_008849887.2	XP_008848109.1	calcium-activated potassium channel subunit alpha-1 isoform X29
XM_008849888.1	XP_008848110.1	calcium-activated potassium channel subunit alpha-1 isoform X30
XM_017802383.1	XP_017657872.1	calcium-activated potassium channel subunit alpha-1 isoform X32
XM_008849870.2	XP_008848092.1	calcium-activated potassium channel subunit alpha-1 isoform X15
XM_008849878.1	XP_008848100.1	calcium-activated potassium channel subunit alpha-1 isoform X21
XM_008849877.2	XP_008848099.1	calcium-activated potassium channel subunit alpha-1 isoform X22
XM_017802382.1	XP_017657871.1	calcium-activated potassium channel subunit alpha-1 isoform X23
XM_029554472.1	XP_029410332.1	calcium-activated potassium channel subunit alpha-1 isoform X4
XM_029554475.1	XP_029410335.1	calcium-activated potassium channel subunit alpha-1 isoform X7
XM_008849866.2	XP_008848088.1	calcium-activated potassium channel subunit alpha-1 isoform X10
XM_008849873.2	XP_008848095.1	calcium-activated potassium channel subunit alpha-1 isoform X13
XM_008849882.3	XP_008848104.1	calcium-activated potassium channel subunit alpha-1 isoform X18
XM_008849885.2	XP_008848107.1	calcium-activated potassium channel subunit alpha-1 isoform X20

Pathways from BioSystems
Gene Ontology

The following Kcnma1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnma1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa135688	XM_029554477.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ONa135687	XM_029554476.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ONa135681	XM_029554470.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONa135682	XM_029554471.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONa135683	XM_029554472.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONa135684	XM_029554473.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONa135685	XM_029554474.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONa135686	XM_029554475.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONa135689	XM_029554478.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONa32274	XM_008849853.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32275	XM_008849854.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32276	XM_008849855.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32277	XM_008849856.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32278	XM_008849857.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32279	XM_008849858.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32280	XM_008849859.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32281	XM_017802381.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32282	XM_008849861.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32283	XM_008849863.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32284	XM_008849864.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32285	XM_008849865.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32286	XM_008849866.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32287	XM_008849867.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32288	XM_008849870.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32289	XM_008849871.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32290	XM_008849872.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32291	XM_008849873.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32292	XM_008849875.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32293	XM_008849876.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32294	XM_008849878.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32295	XM_008849877.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32296	XM_017802382.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32297	XM_008849880.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32298	XM_008849881.3 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32299	XM_008849882.3 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32300	XM_008849883.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32301	XM_008849885.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32302	XM_008849887.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32303	XM_008849888.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32304	XM_008849889.2 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32305	XM_017802383.1 Latest version!	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
ONa32298	XM_008849881.2	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONa32299	XM_008849882.2	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnma1 ( XM_029554477.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029410337.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	ONa135688
Clone ID Related Accession (Same CDS sequence)	XM_029554477.1
Accession Version	XM_029554477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTGT CTTCGTGTTC AGGTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029410337.1
CDS	156..725
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVSSCSGL

Target ORF information:

RefSeq Version	XM_029554477.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029554477.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTGT CTTCGTGTTC AGGTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_029554476.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029410336.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	ONa135687
Clone ID Related Accession (Same CDS sequence)	XM_029554476.1
Accession Version	XM_029554476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTG TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029410336.1
CDS	156..2282
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIYCE

Target ORF information:

RefSeq Version	XM_029554476.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029554476.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTG TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_029554470.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029410330.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONa135681
Clone ID Related Accession (Same CDS sequence)	XM_029554470.1
Accession Version	XM_029554470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGCTAAAC CGGGCAAGTT GCCTTTGGTA 
TCAGTCAATC AGGAAAAAAA CAGTGGGACG CACATTCTAA TGATAACGGA ATTGGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAATGCAA CAAGGATGAA TAGGATGGGC CAAGAAAAGA AATGGTTTAC AGATGAACCG 
GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GTACCCACAT GGCTAACCAG 
ATCAACCAAT ATAAATCCAC AAGCAGCCTG ATCCCTCCAA TCAGAGAAGT TGAAGATGAA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029410330.1
CDS	156..4121
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_029554470.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029554470.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGCTAAAC CGGGCAAGTT GCCTTTGGTA 
TCAGTCAATC AGGAAAAAAA CAGTGGGACG CACATTCTAA TGATAACGGA ATTGGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAATGCAA CAAGGATGAA TAGGATGGGC CAAGAAAAGA AATGGTTTAC AGATGAACCG 
GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GTACCCACAT GGCTAACCAG 
ATCAACCAAT ATAAATCCAC AAGCAGCCTG ATCCCTCCAA TCAGAGAAGT TGAAGATGAA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_029554471.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029410331.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ONa135682
Clone ID Related Accession (Same CDS sequence)	XM_029554471.1
Accession Version	XM_029554471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCCC 
AAGACGTCCA TCTACAAGAG AATGAGCCGA GCATGTTGTT TTGATTGCGG ACGTTCTGAG 
CGTGACTGCT CATGCATGTC AGGCCGTGTG CGTGGTAACG TGGACACCCC TGAGAGAACC 
TTCCCGCTTT CTTCTGTCTC TGTTAATGAT TGCGCCACCA GTTTCCGTGC CTTTGAAGAT 
GAACAGCCGT CAACGCTGTC ACCCAAAAAA AAACAGCGCA ACGGGGGCAT GAGGAACTCG 
CCCAACTCCT CCCCGAAGCT GATGAGGCAT GACCCCTTAC TAATTCCTGG CAATGATCAG 
ATCGACAACA TGGACTCCAA TGTGAAGAAA TACGACTCTA CAGGGATGTT TCACTGGTGC 
GCAGCCAAGG AGATCGAGAA AGTCATCCTG ACTCGAAGCG AAGCTGCCAT GACTGTCCTG 
AGTGGCCACG TCGTAGTCTG CATCTTTGGG GATGTCAGCT CAGCCCTGAT TGGCCTCCGG 
AACCTGGTGA TGCCGCTTCG TGCCAGCAAC TTCCACTACC ATGAGCTCAA ACACATTGTG 
TTTGTGGGAT CCATTGAGTA CCTCAAGCGG GAATGGGAGA CACTTCACAA CTTCCCCAAA 
GTGTCCATAT TGCCGGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGTGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TACTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATCGGGGTCT TGCAGGCTAA TTCCCAAGGA TTCACACCTC CAGGAATGGA CAGATCCTCT 
CCTGACAACA GCCCAGTGCA CGGGATGTTA CGGCAGCCAT CCATCACGAC TGGGGTCAAT 
ATCCCCATCA TCACAGAACT CGCTAAACCG GGCAAGTTGC CTTTGGTATC AGTCAATCAG 
GAAAAAAACA GTGGGACGCA CATTCTAATG ATAACGGAAT TGGTGAATGA CACTAATGTT 
CAGTTTTTGG ACCAAGATGA TGACGATGAC CCCGACACAG AGCTGTACCT CACGCAACCC 
TTTGCGTGTG GGACAGCTTT TGCCGTCAGC GTCCTGGACT CGCTCATGAG TGCGACATAC 
TTCAATGACA ATATCCTCAC CCTGATACGG ACCTTGGTGA CAGGAGGAGC CACACCAGAG 
CTCGAGGCTC TGATTGCTGA GGAGAATGCA CTTCGAGGGG GCTACAGCAC TCCCCAGACA 
CTGGCCAACA GGGACCGTTG CAGAGTGGCC CAGTTAGCTC TGTTGGATGG GCCCTTTGCA 
GACTTAGGGG ATGGTGGTTG TTATGGTGAT TTGTTCTGCA AAGCTCTGAA AACATATAAT 
ATGCTTTGTT TTGGAATTTA CCGGCTGAGA GATGCCCACC TCAGCACCCC CAGCCAGTGT 
ACGAAAAGGT ACGTCATCAC CAACCCCCCC TACGAGTTTG AGCTGGTGCC GACAGACCTG 
ATCTTCTGCC TGATGCAGTT TGACCACAAC GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT 
TCCTCCCACT CATCACAGTC CTCCAGCAAG AAGAGCTCCT CTGTCCACTC CATCCCGTCC 
ACAGCAAACC GGCCAAACCG GCCCAAGTCC AGGGAGTCCC GCGACAAACA GAATGCAACA 
AGGATGAATA GGATGGGCCA AGCAGAAAAG AAATGGTTTA CAGATGAACC GGATAATGCC 
TATCCCAGAA ACATTCAAAT CAAGCCCATG AGTACCCACA TGGCTAACCA GATCAACCAA 
TATAAATCCA CAAGCAGCCT GATCCCTCCA ATCAGAGAAG TTGAAGATGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029410331.1
CDS	156..4112
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_029554471.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029554471.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCCC 
AAGACGTCCA TCTACAAGAG AATGAGCCGA GCATGTTGTT TTGATTGCGG ACGTTCTGAG 
CGTGACTGCT CATGCATGTC AGGCCGTGTG CGTGGTAACG TGGACACCCC TGAGAGAACC 
TTCCCGCTTT CTTCTGTCTC TGTTAATGAT TGCGCCACCA GTTTCCGTGC CTTTGAAGAT 
GAACAGCCGT CAACGCTGTC ACCCAAAAAA AAACAGCGCA ACGGGGGCAT GAGGAACTCG 
CCCAACTCCT CCCCGAAGCT GATGAGGCAT GACCCCTTAC TAATTCCTGG CAATGATCAG 
ATCGACAACA TGGACTCCAA TGTGAAGAAA TACGACTCTA CAGGGATGTT TCACTGGTGC 
GCAGCCAAGG AGATCGAGAA AGTCATCCTG ACTCGAAGCG AAGCTGCCAT GACTGTCCTG 
AGTGGCCACG TCGTAGTCTG CATCTTTGGG GATGTCAGCT CAGCCCTGAT TGGCCTCCGG 
AACCTGGTGA TGCCGCTTCG TGCCAGCAAC TTCCACTACC ATGAGCTCAA ACACATTGTG 
TTTGTGGGAT CCATTGAGTA CCTCAAGCGG GAATGGGAGA CACTTCACAA CTTCCCCAAA 
GTGTCCATAT TGCCGGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGTGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TACTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATCGGGGTCT TGCAGGCTAA TTCCCAAGGA TTCACACCTC CAGGAATGGA CAGATCCTCT 
CCTGACAACA GCCCAGTGCA CGGGATGTTA CGGCAGCCAT CCATCACGAC TGGGGTCAAT 
ATCCCCATCA TCACAGAACT CGCTAAACCG GGCAAGTTGC CTTTGGTATC AGTCAATCAG 
GAAAAAAACA GTGGGACGCA CATTCTAATG ATAACGGAAT TGGTGAATGA CACTAATGTT 
CAGTTTTTGG ACCAAGATGA TGACGATGAC CCCGACACAG AGCTGTACCT CACGCAACCC 
TTTGCGTGTG GGACAGCTTT TGCCGTCAGC GTCCTGGACT CGCTCATGAG TGCGACATAC 
TTCAATGACA ATATCCTCAC CCTGATACGG ACCTTGGTGA CAGGAGGAGC CACACCAGAG 
CTCGAGGCTC TGATTGCTGA GGAGAATGCA CTTCGAGGGG GCTACAGCAC TCCCCAGACA 
CTGGCCAACA GGGACCGTTG CAGAGTGGCC CAGTTAGCTC TGTTGGATGG GCCCTTTGCA 
GACTTAGGGG ATGGTGGTTG TTATGGTGAT TTGTTCTGCA AAGCTCTGAA AACATATAAT 
ATGCTTTGTT TTGGAATTTA CCGGCTGAGA GATGCCCACC TCAGCACCCC CAGCCAGTGT 
ACGAAAAGGT ACGTCATCAC CAACCCCCCC TACGAGTTTG AGCTGGTGCC GACAGACCTG 
ATCTTCTGCC TGATGCAGTT TGACCACAAC GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT 
TCCTCCCACT CATCACAGTC CTCCAGCAAG AAGAGCTCCT CTGTCCACTC CATCCCGTCC 
ACAGCAAACC GGCCAAACCG GCCCAAGTCC AGGGAGTCCC GCGACAAACA GAATGCAACA 
AGGATGAATA GGATGGGCCA AGCAGAAAAG AAATGGTTTA CAGATGAACC GGATAATGCC 
TATCCCAGAA ACATTCAAAT CAAGCCCATG AGTACCCACA TGGCTAACCA GATCAACCAA 
TATAAATCCA CAAGCAGCCT GATCCCTCCA ATCAGAGAAG TTGAAGATGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_029554472.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029410332.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X4, mRNA.

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CloneID	ONa135683
Clone ID Related Accession (Same CDS sequence)	XM_029554472.1
Accession Version	XM_029554472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGTGAATG ACACTAATGT TCAGTTTTTG 
GACCAAGATG ATGACGATGA CCCCGACACA GAGCTGTACC TCACGCAACC CTTTGCGTGT 
GGGACAGCTT TTGCCGTCAG CGTCCTGGAC TCGCTCATGA GTGCGACATA CTTCAATGAC 
AATATCCTCA CCCTGATACG GACCTTGGTG ACAGGAGGAG CCACACCAGA GCTCGAGGCT 
CTGATTGCTG AGGAGAATGC ACTTCGAGGG GGCTACAGCA CTCCCCAGAC ACTGGCCAAC 
AGGGACCGTT GCAGAGTGGC CCAGTTAGCT CTGTTGGATG GGCCCTTTGC AGACTTAGGG 
GATGGTGGTT GTTATGGTGA TTTGTTCTGC AAAGCTCTGA AAACATATAA TATGCTTTGT 
TTTGGAATTT ACCGGCTGAG AGATGCCCAC CTCAGCACCC CCAGCCAGTG TACGAAAAGG 
TACGTCATCA CCAACCCCCC CTACGAGTTT GAGCTGGTGC CGACAGACCT GATCTTCTGC 
CTGATGCAGT TTGACCACAA CGCCGGCCAG TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC 
TCATCACAGT CCTCCAGCAA GAAGAGCTCC TCTGTCCACT CCATCCCGTC CACAGCAAAC 
CGGCCAAACC GGCCCAAGTC CAGGGAGTCC CGCGACAAAC AGAATGCAAC AAGGATGAAT 
AGGATGGGCC AAGCAGAAAA GAAATGGTTT ACAGATGAAC CGGATAATGC CTATCCCAGA 
AACATTCAAA TCAAGCCCAT GAGTACCCAC ATGGCTAACC AGATCAACCA ATATAAATCC 
ACAAGCAGCC TGATCCCTCC AATCAGAGAA GTTGAAGATG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029410332.1
CDS	156..4043
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_029554472.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029554472.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGTGAATG ACACTAATGT TCAGTTTTTG 
GACCAAGATG ATGACGATGA CCCCGACACA GAGCTGTACC TCACGCAACC CTTTGCGTGT 
GGGACAGCTT TTGCCGTCAG CGTCCTGGAC TCGCTCATGA GTGCGACATA CTTCAATGAC 
AATATCCTCA CCCTGATACG GACCTTGGTG ACAGGAGGAG CCACACCAGA GCTCGAGGCT 
CTGATTGCTG AGGAGAATGC ACTTCGAGGG GGCTACAGCA CTCCCCAGAC ACTGGCCAAC 
AGGGACCGTT GCAGAGTGGC CCAGTTAGCT CTGTTGGATG GGCCCTTTGC AGACTTAGGG 
GATGGTGGTT GTTATGGTGA TTTGTTCTGC AAAGCTCTGA AAACATATAA TATGCTTTGT 
TTTGGAATTT ACCGGCTGAG AGATGCCCAC CTCAGCACCC CCAGCCAGTG TACGAAAAGG 
TACGTCATCA CCAACCCCCC CTACGAGTTT GAGCTGGTGC CGACAGACCT GATCTTCTGC 
CTGATGCAGT TTGACCACAA CGCCGGCCAG TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC 
TCATCACAGT CCTCCAGCAA GAAGAGCTCC TCTGTCCACT CCATCCCGTC CACAGCAAAC 
CGGCCAAACC GGCCCAAGTC CAGGGAGTCC CGCGACAAAC AGAATGCAAC AAGGATGAAT 
AGGATGGGCC AAGCAGAAAA GAAATGGTTT ACAGATGAAC CGGATAATGC CTATCCCAGA 
AACATTCAAA TCAAGCCCAT GAGTACCCAC ATGGCTAACC AGATCAACCA ATATAAATCC 
ACAAGCAGCC TGATCCCTCC AATCAGAGAA GTTGAAGATG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_029554473.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029410333.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ONa135684
Clone ID Related Accession (Same CDS sequence)	XM_029554473.1
Accession Version	XM_029554473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGTGAATG ACACTAATGT TCAGTTTTTG 
GACCAAGATG ATGACGATGA CCCCGACACA GAGCTGTACC TCACGCAACC CTTTGCGTGT 
GGGACAGCTT TTGCCGTCAG CGTCCTGGAC TCGCTCATGA GTGCGACATA CTTCAATGAC 
AATATCCTCA CCCTGATACG GACCTTGGTG ACAGGAGGAG CCACACCAGA GCTCGAGGCT 
CTGATTGCTG AGGAGAATGC ACTTCGAGGG GGCTACAGCA CTCCCCAGAC ACTGGCCAAC 
AGGGACCGTT GCAGAGTGGC CCAGTTAGCT CTGTTGGATG GGCCCTTTGC AGACTTAGGG 
GATGGTGGTT GTTATGGTGA TTTGTTCTGC AAAGCTCTGA AAACATATAA TATGCTTTGT 
TTTGGAATTT ACCGGCTGAG AGATGCCCAC CTCAGCACCC CCAGCCAGTG TACGAAAAGG 
TACGTCATCA CCAACCCCCC CTACGAGTTT GAGCTGGTGC CGACAGACCT GATCTTCTGC 
CTGATGCAGT TTGACCACAA CGCCGGCCAG TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC 
TCATCACAGT CCTCCAGCAA GAAGAGCTCC TCTGTCCACT CCATCCCGTC CACAGCAAAC 
CGGCCAAACC GGCCCAAGTC CAGGGAGTCC CGCGACAAAC AGAATGCAAC AAGGATGAAT 
AGGATGGGCC AAGAAAAGAA ATGGTTTACA GATGAACCGG ATAATGCCTA TCCCAGAAAC 
ATTCAAATCA AGCCCATGAG TACCCACATG GCTAACCAGA TCAACCAATA TAAATCCACA 
AGCAGCCTGA TCCCTCCAAT CAGAGAAGTT GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029410333.1
CDS	156..4040
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_029554473.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029554473.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGTGAATG ACACTAATGT TCAGTTTTTG 
GACCAAGATG ATGACGATGA CCCCGACACA GAGCTGTACC TCACGCAACC CTTTGCGTGT 
GGGACAGCTT TTGCCGTCAG CGTCCTGGAC TCGCTCATGA GTGCGACATA CTTCAATGAC 
AATATCCTCA CCCTGATACG GACCTTGGTG ACAGGAGGAG CCACACCAGA GCTCGAGGCT 
CTGATTGCTG AGGAGAATGC ACTTCGAGGG GGCTACAGCA CTCCCCAGAC ACTGGCCAAC 
AGGGACCGTT GCAGAGTGGC CCAGTTAGCT CTGTTGGATG GGCCCTTTGC AGACTTAGGG 
GATGGTGGTT GTTATGGTGA TTTGTTCTGC AAAGCTCTGA AAACATATAA TATGCTTTGT 
TTTGGAATTT ACCGGCTGAG AGATGCCCAC CTCAGCACCC CCAGCCAGTG TACGAAAAGG 
TACGTCATCA CCAACCCCCC CTACGAGTTT GAGCTGGTGC CGACAGACCT GATCTTCTGC 
CTGATGCAGT TTGACCACAA CGCCGGCCAG TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC 
TCATCACAGT CCTCCAGCAA GAAGAGCTCC TCTGTCCACT CCATCCCGTC CACAGCAAAC 
CGGCCAAACC GGCCCAAGTC CAGGGAGTCC CGCGACAAAC AGAATGCAAC AAGGATGAAT 
AGGATGGGCC AAGAAAAGAA ATGGTTTACA GATGAACCGG ATAATGCCTA TCCCAGAAAC 
ATTCAAATCA AGCCCATGAG TACCCACATG GCTAACCAGA TCAACCAATA TAAATCCACA 
AGCAGCCTGA TCCCTCCAAT CAGAGAAGTT GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_029554474.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029410334.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ONa135685
Clone ID Related Accession (Same CDS sequence)	XM_029554474.1
Accession Version	XM_029554474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGCTAAAC CGGGCAAGTT GCCTTTGGTA 
TCAGTCAATC AGGAAAAAAA CAGTGGGACG CACATTCTAA TGATAACGGA ATTGGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAACAGAA AAGAAATGGT TTACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029410334.1
CDS	156..3965
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_029554474.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029554474.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGCTAAAC CGGGCAAGTT GCCTTTGGTA 
TCAGTCAATC AGGAAAAAAA CAGTGGGACG CACATTCTAA TGATAACGGA ATTGGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAACAGAA AAGAAATGGT TTACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_029554475.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029410335.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X7, mRNA.

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CloneID	ONa135686
Clone ID Related Accession (Same CDS sequence)	XM_029554475.1
Accession Version	XM_029554475.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGCTAAAC CGGGCAAGTT GCCTTTGGTA 
TCAGTCAATC AGGAAAAAAA CAGTGGGACG CACATTCTAA TGATAACGGA ATTGGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAAAAAAG AAATGGTTTA CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029410335.1
CDS	156..3962
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR

Target ORF information:

RefSeq Version	XM_029554475.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029554475.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGCTAAAC CGGGCAAGTT GCCTTTGGTA 
TCAGTCAATC AGGAAAAAAA CAGTGGGACG CACATTCTAA TGATAACGGA ATTGGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAAAAAAG AAATGGTTTA CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_029554478.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029410338.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X23, mRNA.

Price & Availability↑

CloneID	ONa135689
Clone ID Related Accession (Same CDS sequence)	XM_029554478.1
Accession Version	XM_029554478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCCC 
AAGACGTCCA TCTACAAGAG AATGAGCCGA GCATGTTGTT TTGATTGCGG ACGTTCTGAG 
CGTGACTGCT CATGCATGTC AGGCCGTGTG CGTGGTAACG TGGACACCCC TGAGAGAACC 
TTCCCGCTTT CTTCTGTCTC TGTTAATGAT TGCGCCACCA GTTTCCGTGC CTTTGAAGAT 
GAACAGCCGT CAACGCTGTC ACCCAAAAAA AAACAGCGCA ACGGGGGCAT GAGGAACTCG 
CCCAACTCCT CCCCGAAGCT GATGAGGCAT GACCCCTTAC TAATTCCTGG CAATGATCAG 
ATCGACAACA TGGACTCCAA TGTGAAGAAA TACGACTCTA CAGGGATGTT TCACTGGTGC 
GCAGCCAAGG AGATCGAGAA AGTCATCCTG ACTCGAAGCG AAGCTGCCAT GACTGTCCTG 
AGTGGCCACG TCGTAGTCTG CATCTTTGGG GATGTCAGCT CAGCCCTGAT TGGCCTCCGG 
AACCTGGTGA TGCCGCTTCG TGCCAGCAAC TTCCACTACC ATGAGCTCAA ACACATTGTG 
TTTGTGGGAT CCATTGAGTA CCTCAAGCGG GAATGGGAGA CACTTCACAA CTTCCCCAAA 
GTGTCCATAT TGCCGGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGTGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TACTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATCGGGGTCT TGCAGGCTAA TTCCCAAGGA TTCACACCTC CAGGAATGGA CAGATCCTCT 
CCTGACAACA GCCCAGTGCA CGGGATGTTA CGGCAGCCAT CCATCACGAC TGGGGTCAAT 
ATCCCCATCA TCACAGAACT CGCTAAACCG GGCAAGTTGC CTTTGGTATC AGTCAATCAG 
GAAAAAAACA GTGGGACGCA CATTCTAATG ATAACGGAAT TGGTGAATGA CACTAATGTT 
CAGTTTTTGG ACCAAGATGA TGACGATGAC CCCGACACAG AGCTGTACCT CACGCAACCC 
TTTGCGTGTG GGACAGCTTT TGCCGTCAGC GTCCTGGACT CGCTCATGAG TGCGACATAC 
TTCAATGACA ATATCCTCAC CCTGATACGG ACCTTGGTGA CAGGAGGAGC CACACCAGAG 
CTCGAGGCTC TGATTGCTGA GGAGAATGCA CTTCGAGGGG GCTACAGCAC TCCCCAGACA 
CTGGCCAACA GGGACCGTTG CAGAGTGGCC CAGTTAGCTC TGTTGGATGG GCCCTTTGCA 
GACTTAGGGG ATGGTGGTTG TTATGGTGAT TTGTTCTGCA AAGCTCTGAA AACATATAAT 
ATGCTTTGTT TTGGAATTTA CCGGCTGAGA GATGCCCACC TCAGCACCCC CAGCCAGTGT 
ACGAAAAGGT ACGTCATCAC CAACCCCCCC TACGAGTTTG AGCTGGTGCC GACAGACCTG 
ATCTTCTGCC TGATGCAGTT TGACCACAAC GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT 
TCCTCCCACT CATCACAGTC CTCCAGCAAG AAGAGCTCCT CTGTCCACTC CATCCCGTCC 
ACAGCAAACC GGCCAAACCG GCCCAAGTCC AGGGAGTCCC GCGACAAACA GAAAAAAGAA 
ATGGTTTACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029410338.1
CDS	156..3950
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR

Target ORF information:

RefSeq Version	XM_029554478.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029554478.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCCC 
AAGACGTCCA TCTACAAGAG AATGAGCCGA GCATGTTGTT TTGATTGCGG ACGTTCTGAG 
CGTGACTGCT CATGCATGTC AGGCCGTGTG CGTGGTAACG TGGACACCCC TGAGAGAACC 
TTCCCGCTTT CTTCTGTCTC TGTTAATGAT TGCGCCACCA GTTTCCGTGC CTTTGAAGAT 
GAACAGCCGT CAACGCTGTC ACCCAAAAAA AAACAGCGCA ACGGGGGCAT GAGGAACTCG 
CCCAACTCCT CCCCGAAGCT GATGAGGCAT GACCCCTTAC TAATTCCTGG CAATGATCAG 
ATCGACAACA TGGACTCCAA TGTGAAGAAA TACGACTCTA CAGGGATGTT TCACTGGTGC 
GCAGCCAAGG AGATCGAGAA AGTCATCCTG ACTCGAAGCG AAGCTGCCAT GACTGTCCTG 
AGTGGCCACG TCGTAGTCTG CATCTTTGGG GATGTCAGCT CAGCCCTGAT TGGCCTCCGG 
AACCTGGTGA TGCCGCTTCG TGCCAGCAAC TTCCACTACC ATGAGCTCAA ACACATTGTG 
TTTGTGGGAT CCATTGAGTA CCTCAAGCGG GAATGGGAGA CACTTCACAA CTTCCCCAAA 
GTGTCCATAT TGCCGGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGTGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TACTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATCGGGGTCT TGCAGGCTAA TTCCCAAGGA TTCACACCTC CAGGAATGGA CAGATCCTCT 
CCTGACAACA GCCCAGTGCA CGGGATGTTA CGGCAGCCAT CCATCACGAC TGGGGTCAAT 
ATCCCCATCA TCACAGAACT CGCTAAACCG GGCAAGTTGC CTTTGGTATC AGTCAATCAG 
GAAAAAAACA GTGGGACGCA CATTCTAATG ATAACGGAAT TGGTGAATGA CACTAATGTT 
CAGTTTTTGG ACCAAGATGA TGACGATGAC CCCGACACAG AGCTGTACCT CACGCAACCC 
TTTGCGTGTG GGACAGCTTT TGCCGTCAGC GTCCTGGACT CGCTCATGAG TGCGACATAC 
TTCAATGACA ATATCCTCAC CCTGATACGG ACCTTGGTGA CAGGAGGAGC CACACCAGAG 
CTCGAGGCTC TGATTGCTGA GGAGAATGCA CTTCGAGGGG GCTACAGCAC TCCCCAGACA 
CTGGCCAACA GGGACCGTTG CAGAGTGGCC CAGTTAGCTC TGTTGGATGG GCCCTTTGCA 
GACTTAGGGG ATGGTGGTTG TTATGGTGAT TTGTTCTGCA AAGCTCTGAA AACATATAAT 
ATGCTTTGTT TTGGAATTTA CCGGCTGAGA GATGCCCACC TCAGCACCCC CAGCCAGTGT 
ACGAAAAGGT ACGTCATCAC CAACCCCCCC TACGAGTTTG AGCTGGTGCC GACAGACCTG 
ATCTTCTGCC TGATGCAGTT TGACCACAAC GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT 
TCCTCCCACT CATCACAGTC CTCCAGCAAG AAGAGCTCCT CTGTCCACTC CATCCCGTCC 
ACAGCAAACC GGCCAAACCG GCCCAAGTCC AGGGAGTCCC GCGACAAACA GAAAAAAGAA 
ATGGTTTACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849853.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848075.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONa32274
Clone ID Related Accession (Same CDS sequence)	XM_008849853.2
Accession Version	XM_008849853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGCTAAACC GGGCAAGTTG 
CCTTTGGTAT CAGTCAATCA GGAAAAAAAC AGTGGGACGC ACATTCTAAT GATAACGGAA 
TTGGTGAATG ACACTAATGT TCAGTTTTTG GACCAAGATG ATGACGATGA CCCCGACACA 
GAGCTGTACC TCACGCAACC CTTTGCGTGT GGGACAGCTT TTGCCGTCAG CGTCCTGGAC 
TCGCTCATGA GTGCGACATA CTTCAATGAC AATATCCTCA CCCTGATACG GACCTTGGTG 
ACAGGAGGAG CCACACCAGA GCTCGAGGCT CTGATTGCTG AGGAGAATGC ACTTCGAGGG 
GGCTACAGCA CTCCCCAGAC ACTGGCCAAC AGGGACCGTT GCAGAGTGGC CCAGTTAGCT 
CTGTTGGATG GGCCCTTTGC AGACTTAGGG GATGGTGGTT GTTATGGTGA TTTGTTCTGC 
AAAGCTCTGA AAACATATAA TATGCTTTGT TTTGGAATTT ACCGGCTGAG AGATGCCCAC 
CTCAGCACCC CCAGCCAGTG TACGAAAAGG TACGTCATCA CCAACCCCCC CTACGAGTTT 
GAGCTGGTGC CGACAGACCT GATCTTCTGC CTGATGCAGT TTGACCACAA CGCCGGCCAG 
TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC TCATCACAGT CCTCCAGCAA GAAGAGCTCC 
TCTGTCCACT CCATCCCGTC CACAGCAAAC CGGCCAAACC GGCCCAAGTC CAGGGAGTCC 
CGCGACAAAC AGAATGCAAC AAGGATGAAT AGGATGGGCC AAGCAGAAAA GAAATGGTTT 
ACAGATGAAC CGGATAATGC CTATCCCAGA AACATTCAAA TCAAGCCCAT GAGTACCCAC 
ATGGCTAACC AGATCAACCA ATATAAATCC ACAAGCAGCC TGATCCCTCC AATCAGAGAA 
GTTGAAGATG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848075.1
CDS	156..4133
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIYSKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_008849853.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849853.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGCTAAACC GGGCAAGTTG 
CCTTTGGTAT CAGTCAATCA GGAAAAAAAC AGTGGGACGC ACATTCTAAT GATAACGGAA 
TTGGTGAATG ACACTAATGT TCAGTTTTTG GACCAAGATG ATGACGATGA CCCCGACACA 
GAGCTGTACC TCACGCAACC CTTTGCGTGT GGGACAGCTT TTGCCGTCAG CGTCCTGGAC 
TCGCTCATGA GTGCGACATA CTTCAATGAC AATATCCTCA CCCTGATACG GACCTTGGTG 
ACAGGAGGAG CCACACCAGA GCTCGAGGCT CTGATTGCTG AGGAGAATGC ACTTCGAGGG 
GGCTACAGCA CTCCCCAGAC ACTGGCCAAC AGGGACCGTT GCAGAGTGGC CCAGTTAGCT 
CTGTTGGATG GGCCCTTTGC AGACTTAGGG GATGGTGGTT GTTATGGTGA TTTGTTCTGC 
AAAGCTCTGA AAACATATAA TATGCTTTGT TTTGGAATTT ACCGGCTGAG AGATGCCCAC 
CTCAGCACCC CCAGCCAGTG TACGAAAAGG TACGTCATCA CCAACCCCCC CTACGAGTTT 
GAGCTGGTGC CGACAGACCT GATCTTCTGC CTGATGCAGT TTGACCACAA CGCCGGCCAG 
TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC TCATCACAGT CCTCCAGCAA GAAGAGCTCC 
TCTGTCCACT CCATCCCGTC CACAGCAAAC CGGCCAAACC GGCCCAAGTC CAGGGAGTCC 
CGCGACAAAC AGAATGCAAC AAGGATGAAT AGGATGGGCC AAGCAGAAAA GAAATGGTTT 
ACAGATGAAC CGGATAATGC CTATCCCAGA AACATTCAAA TCAAGCCCAT GAGTACCCAC 
ATGGCTAACC AGATCAACCA ATATAAATCC ACAAGCAGCC TGATCCCTCC AATCAGAGAA 
GTTGAAGATG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849854.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848076.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X2, mRNA.

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CloneID	ONa32275
Clone ID Related Accession (Same CDS sequence)	XM_008849854.2
Accession Version	XM_008849854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGCTAAACC GGGCAAGTTG 
CCTTTGGTAT CAGTCAATCA GGAAAAAAAC AGTGGGACGC ACATTCTAAT GATAACGGAA 
TTGGTGAATG ACACTAATGT TCAGTTTTTG GACCAAGATG ATGACGATGA CCCCGACACA 
GAGCTGTACC TCACGCAACC CTTTGCGTGT GGGACAGCTT TTGCCGTCAG CGTCCTGGAC 
TCGCTCATGA GTGCGACATA CTTCAATGAC AATATCCTCA CCCTGATACG GACCTTGGTG 
ACAGGAGGAG CCACACCAGA GCTCGAGGCT CTGATTGCTG AGGAGAATGC ACTTCGAGGG 
GGCTACAGCA CTCCCCAGAC ACTGGCCAAC AGGGACCGTT GCAGAGTGGC CCAGTTAGCT 
CTGTTGGATG GGCCCTTTGC AGACTTAGGG GATGGTGGTT GTTATGGTGA TTTGTTCTGC 
AAAGCTCTGA AAACATATAA TATGCTTTGT TTTGGAATTT ACCGGCTGAG AGATGCCCAC 
CTCAGCACCC CCAGCCAGTG TACGAAAAGG TACGTCATCA CCAACCCCCC CTACGAGTTT 
GAGCTGGTGC CGACAGACCT GATCTTCTGC CTGATGCAGT TTGACCACAA CGCCGGCCAG 
TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC TCATCACAGT CCTCCAGCAA GAAGAGCTCC 
TCTGTCCACT CCATCCCGTC CACAGCAAAC CGGCCAAACC GGCCCAAGTC CAGGGAGTCC 
CGCGACAAAC AGAATGCAAC AAGGATGAAT AGGATGGGCC AAGAAAAGAA ATGGTTTACA 
GATGAACCGG ATAATGCCTA TCCCAGAAAC ATTCAAATCA AGCCCATGAG TACCCACATG 
GCTAACCAGA TCAACCAATA TAAATCCACA AGCAGCCTGA TCCCTCCAAT CAGAGAAGTT 
GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848076.1
CDS	156..4130
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIYSKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_008849854.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849854.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGCTAAACC GGGCAAGTTG 
CCTTTGGTAT CAGTCAATCA GGAAAAAAAC AGTGGGACGC ACATTCTAAT GATAACGGAA 
TTGGTGAATG ACACTAATGT TCAGTTTTTG GACCAAGATG ATGACGATGA CCCCGACACA 
GAGCTGTACC TCACGCAACC CTTTGCGTGT GGGACAGCTT TTGCCGTCAG CGTCCTGGAC 
TCGCTCATGA GTGCGACATA CTTCAATGAC AATATCCTCA CCCTGATACG GACCTTGGTG 
ACAGGAGGAG CCACACCAGA GCTCGAGGCT CTGATTGCTG AGGAGAATGC ACTTCGAGGG 
GGCTACAGCA CTCCCCAGAC ACTGGCCAAC AGGGACCGTT GCAGAGTGGC CCAGTTAGCT 
CTGTTGGATG GGCCCTTTGC AGACTTAGGG GATGGTGGTT GTTATGGTGA TTTGTTCTGC 
AAAGCTCTGA AAACATATAA TATGCTTTGT TTTGGAATTT ACCGGCTGAG AGATGCCCAC 
CTCAGCACCC CCAGCCAGTG TACGAAAAGG TACGTCATCA CCAACCCCCC CTACGAGTTT 
GAGCTGGTGC CGACAGACCT GATCTTCTGC CTGATGCAGT TTGACCACAA CGCCGGCCAG 
TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC TCATCACAGT CCTCCAGCAA GAAGAGCTCC 
TCTGTCCACT CCATCCCGTC CACAGCAAAC CGGCCAAACC GGCCCAAGTC CAGGGAGTCC 
CGCGACAAAC AGAATGCAAC AAGGATGAAT AGGATGGGCC AAGAAAAGAA ATGGTTTACA 
GATGAACCGG ATAATGCCTA TCCCAGAAAC ATTCAAATCA AGCCCATGAG TACCCACATG 
GCTAACCAGA TCAACCAATA TAAATCCACA AGCAGCCTGA TCCCTCCAAT CAGAGAAGTT 
GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849855.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848077.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X1, mRNA.

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CloneID	ONa32276
Clone ID Related Accession (Same CDS sequence)	XM_008849855.2
Accession Version	XM_008849855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGCTAAAC CGGGCAAGTT GCCTTTGGTA 
TCAGTCAATC AGGAAAAAAA CAGTGGGACG CACATTCTAA TGATAACGGA ATTGGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAATGCAA CAAGGATGAA TAGGATGGGC CAAGCAGAAA AGAAATGGTT TACAGATGAA 
CCGGATAATG CCTATCCCAG AAACATTCAA ATCAAGCCCA TGAGTACCCA CATGGCTAAC 
CAGATCAACC AATATAAATC CACAAGCAGC CTGATCCCTC CAATCAGAGA AGTTGAAGAT 
GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848077.1
CDS	156..4124
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_008849855.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849855.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGCTAAAC CGGGCAAGTT GCCTTTGGTA 
TCAGTCAATC AGGAAAAAAA CAGTGGGACG CACATTCTAA TGATAACGGA ATTGGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAATGCAA CAAGGATGAA TAGGATGGGC CAAGCAGAAA AGAAATGGTT TACAGATGAA 
CCGGATAATG CCTATCCCAG AAACATTCAA ATCAAGCCCA TGAGTACCCA CATGGCTAAC 
CAGATCAACC AATATAAATC CACAAGCAGC CTGATCCCTC CAATCAGAGA AGTTGAAGAT 
GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849856.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848078.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ONa32277
Clone ID Related Accession (Same CDS sequence)	XM_008849856.2
Accession Version	XM_008849856.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTG 
ATCTATTCCA AGACGTCCAT CTACAAGAGA ATGAGCCGAG CATGTTGTTT TGATTGCGGA 
CGTTCTGAGC GTGACTGCTC ATGCATGTCA GGCCGTGTGC GTGGTAACGT GGACACCCCT 
GAGAGAACCT TCCCGCTTTC TTCTGTCTCT GTTAATGATT GCGCCACCAG TTTCCGTGCC 
TTTGAAGATG AACAGCCGTC AACGCTGTCA CCCAAAAAAA AACAGCGCAA CGGGGGCATG 
AGGAACTCGC CCAACTCCTC CCCGAAGCTG ATGAGGCATG ACCCCTTACT AATTCCTGGC 
AATGATCAGA TCGACAACAT GGACTCCAAT GTGAAGAAAT ACGACTCTAC AGGGATGTTT 
CACTGGTGCG CAGCCAAGGA GATCGAGAAA GTCATCCTGA CTCGAAGCGA AGCTGCCATG 
ACTGTCCTGA GTGGCCACGT CGTAGTCTGC ATCTTTGGGG ATGTCAGCTC AGCCCTGATT 
GGCCTCCGGA ACCTGGTGAT GCCGCTTCGT GCCAGCAACT TCCACTACCA TGAGCTCAAA 
CACATTGTGT TTGTGGGATC CATTGAGTAC CTCAAGCGGG AATGGGAGAC ACTTCACAAC 
TTCCCCAAAG TGTCCATATT GCCGGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGTGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
ACTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TCGGGGTCTT GCAGGCTAAT TCCCAAGGAT TCACACCTCC AGGAATGGAC 
AGATCCTCTC CTGACAACAG CCCAGTGCAC GGGATGTTAC GGCAGCCATC CATCACGACT 
GGGGTCAATA TCCCCATCAT CACAGAACTC GCTAAACCGG GCAAGTTGCC TTTGGTATCA 
GTCAATCAGG AAAAAAACAG TGGGACGCAC ATTCTAATGA TAACGGAATT GGTGAATGAC 
ACTAATGTTC AGTTTTTGGA CCAAGATGAT GACGATGACC CCGACACAGA GCTGTACCTC 
ACGCAACCCT TTGCGTGTGG GACAGCTTTT GCCGTCAGCG TCCTGGACTC GCTCATGAGT 
GCGACATACT TCAATGACAA TATCCTCACC CTGATACGGA CCTTGGTGAC AGGAGGAGCC 
ACACCAGAGC TCGAGGCTCT GATTGCTGAG GAGAATGCAC TTCGAGGGGG CTACAGCACT 
CCCCAGACAC TGGCCAACAG GGACCGTTGC AGAGTGGCCC AGTTAGCTCT GTTGGATGGG 
CCCTTTGCAG ACTTAGGGGA TGGTGGTTGT TATGGTGATT TGTTCTGCAA AGCTCTGAAA 
ACATATAATA TGCTTTGTTT TGGAATTTAC CGGCTGAGAG ATGCCCACCT CAGCACCCCC 
AGCCAGTGTA CGAAAAGGTA CGTCATCACC AACCCCCCCT ACGAGTTTGA GCTGGTGCCG 
ACAGACCTGA TCTTCTGCCT GATGCAGTTT GACCACAACG CCGGCCAGTC CCGGGCCAGC 
CTGTCCCATT CCTCCCACTC ATCACAGTCC TCCAGCAAGA AGAGCTCCTC TGTCCACTCC 
ATCCCGTCCA CAGCAAACCG GCCAAACCGG CCCAAGTCCA GGGAGTCCCG CGACAAACAG 
AATGCAACAA GGATGAATAG GATGGGCCAA GCAGAAAAGA AATGGTTTAC AGATGAACCG 
GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GTACCCACAT GGCTAACCAG 
ATCAACCAAT ATAAATCCAC AAGCAGCCTG ATCCCTCCAA TCAGAGAAGT TGAAGATGAA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848078.1
CDS	156..4121
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIYSKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_008849856.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849856.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTG 
ATCTATTCCA AGACGTCCAT CTACAAGAGA ATGAGCCGAG CATGTTGTTT TGATTGCGGA 
CGTTCTGAGC GTGACTGCTC ATGCATGTCA GGCCGTGTGC GTGGTAACGT GGACACCCCT 
GAGAGAACCT TCCCGCTTTC TTCTGTCTCT GTTAATGATT GCGCCACCAG TTTCCGTGCC 
TTTGAAGATG AACAGCCGTC AACGCTGTCA CCCAAAAAAA AACAGCGCAA CGGGGGCATG 
AGGAACTCGC CCAACTCCTC CCCGAAGCTG ATGAGGCATG ACCCCTTACT AATTCCTGGC 
AATGATCAGA TCGACAACAT GGACTCCAAT GTGAAGAAAT ACGACTCTAC AGGGATGTTT 
CACTGGTGCG CAGCCAAGGA GATCGAGAAA GTCATCCTGA CTCGAAGCGA AGCTGCCATG 
ACTGTCCTGA GTGGCCACGT CGTAGTCTGC ATCTTTGGGG ATGTCAGCTC AGCCCTGATT 
GGCCTCCGGA ACCTGGTGAT GCCGCTTCGT GCCAGCAACT TCCACTACCA TGAGCTCAAA 
CACATTGTGT TTGTGGGATC CATTGAGTAC CTCAAGCGGG AATGGGAGAC ACTTCACAAC 
TTCCCCAAAG TGTCCATATT GCCGGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGTGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
ACTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TCGGGGTCTT GCAGGCTAAT TCCCAAGGAT TCACACCTCC AGGAATGGAC 
AGATCCTCTC CTGACAACAG CCCAGTGCAC GGGATGTTAC GGCAGCCATC CATCACGACT 
GGGGTCAATA TCCCCATCAT CACAGAACTC GCTAAACCGG GCAAGTTGCC TTTGGTATCA 
GTCAATCAGG AAAAAAACAG TGGGACGCAC ATTCTAATGA TAACGGAATT GGTGAATGAC 
ACTAATGTTC AGTTTTTGGA CCAAGATGAT GACGATGACC CCGACACAGA GCTGTACCTC 
ACGCAACCCT TTGCGTGTGG GACAGCTTTT GCCGTCAGCG TCCTGGACTC GCTCATGAGT 
GCGACATACT TCAATGACAA TATCCTCACC CTGATACGGA CCTTGGTGAC AGGAGGAGCC 
ACACCAGAGC TCGAGGCTCT GATTGCTGAG GAGAATGCAC TTCGAGGGGG CTACAGCACT 
CCCCAGACAC TGGCCAACAG GGACCGTTGC AGAGTGGCCC AGTTAGCTCT GTTGGATGGG 
CCCTTTGCAG ACTTAGGGGA TGGTGGTTGT TATGGTGATT TGTTCTGCAA AGCTCTGAAA 
ACATATAATA TGCTTTGTTT TGGAATTTAC CGGCTGAGAG ATGCCCACCT CAGCACCCCC 
AGCCAGTGTA CGAAAAGGTA CGTCATCACC AACCCCCCCT ACGAGTTTGA GCTGGTGCCG 
ACAGACCTGA TCTTCTGCCT GATGCAGTTT GACCACAACG CCGGCCAGTC CCGGGCCAGC 
CTGTCCCATT CCTCCCACTC ATCACAGTCC TCCAGCAAGA AGAGCTCCTC TGTCCACTCC 
ATCCCGTCCA CAGCAAACCG GCCAAACCGG CCCAAGTCCA GGGAGTCCCG CGACAAACAG 
AATGCAACAA GGATGAATAG GATGGGCCAA GCAGAAAAGA AATGGTTTAC AGATGAACCG 
GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GTACCCACAT GGCTAACCAG 
ATCAACCAAT ATAAATCCAC AAGCAGCCTG ATCCCTCCAA TCAGAGAAGT TGAAGATGAA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849857.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848079.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ONa32278
Clone ID Related Accession (Same CDS sequence)	XM_008849857.2
Accession Version	XM_008849857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGTGAATGA CACTAATGTT 
CAGTTTTTGG ACCAAGATGA TGACGATGAC CCCGACACAG AGCTGTACCT CACGCAACCC 
TTTGCGTGTG GGACAGCTTT TGCCGTCAGC GTCCTGGACT CGCTCATGAG TGCGACATAC 
TTCAATGACA ATATCCTCAC CCTGATACGG ACCTTGGTGA CAGGAGGAGC CACACCAGAG 
CTCGAGGCTC TGATTGCTGA GGAGAATGCA CTTCGAGGGG GCTACAGCAC TCCCCAGACA 
CTGGCCAACA GGGACCGTTG CAGAGTGGCC CAGTTAGCTC TGTTGGATGG GCCCTTTGCA 
GACTTAGGGG ATGGTGGTTG TTATGGTGAT TTGTTCTGCA AAGCTCTGAA AACATATAAT 
ATGCTTTGTT TTGGAATTTA CCGGCTGAGA GATGCCCACC TCAGCACCCC CAGCCAGTGT 
ACGAAAAGGT ACGTCATCAC CAACCCCCCC TACGAGTTTG AGCTGGTGCC GACAGACCTG 
ATCTTCTGCC TGATGCAGTT TGACCACAAC GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT 
TCCTCCCACT CATCACAGTC CTCCAGCAAG AAGAGCTCCT CTGTCCACTC CATCCCGTCC 
ACAGCAAACC GGCCAAACCG GCCCAAGTCC AGGGAGTCCC GCGACAAACA GAATGCAACA 
AGGATGAATA GGATGGGCCA AGCAGAAAAG AAATGGTTTA CAGATGAACC GGATAATGCC 
TATCCCAGAA ACATTCAAAT CAAGCCCATG AGTACCCACA TGGCTAACCA GATCAACCAA 
TATAAATCCA CAAGCAGCCT GATCCCTCCA ATCAGAGAAG TTGAAGATGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848079.1
CDS	156..4052
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIYSKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_008849857.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849857.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGTGAATGA CACTAATGTT 
CAGTTTTTGG ACCAAGATGA TGACGATGAC CCCGACACAG AGCTGTACCT CACGCAACCC 
TTTGCGTGTG GGACAGCTTT TGCCGTCAGC GTCCTGGACT CGCTCATGAG TGCGACATAC 
TTCAATGACA ATATCCTCAC CCTGATACGG ACCTTGGTGA CAGGAGGAGC CACACCAGAG 
CTCGAGGCTC TGATTGCTGA GGAGAATGCA CTTCGAGGGG GCTACAGCAC TCCCCAGACA 
CTGGCCAACA GGGACCGTTG CAGAGTGGCC CAGTTAGCTC TGTTGGATGG GCCCTTTGCA 
GACTTAGGGG ATGGTGGTTG TTATGGTGAT TTGTTCTGCA AAGCTCTGAA AACATATAAT 
ATGCTTTGTT TTGGAATTTA CCGGCTGAGA GATGCCCACC TCAGCACCCC CAGCCAGTGT 
ACGAAAAGGT ACGTCATCAC CAACCCCCCC TACGAGTTTG AGCTGGTGCC GACAGACCTG 
ATCTTCTGCC TGATGCAGTT TGACCACAAC GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT 
TCCTCCCACT CATCACAGTC CTCCAGCAAG AAGAGCTCCT CTGTCCACTC CATCCCGTCC 
ACAGCAAACC GGCCAAACCG GCCCAAGTCC AGGGAGTCCC GCGACAAACA GAATGCAACA 
AGGATGAATA GGATGGGCCA AGCAGAAAAG AAATGGTTTA CAGATGAACC GGATAATGCC 
TATCCCAGAA ACATTCAAAT CAAGCCCATG AGTACCCACA TGGCTAACCA GATCAACCAA 
TATAAATCCA CAAGCAGCCT GATCCCTCCA ATCAGAGAAG TTGAAGATGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849858.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848080.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X6, mRNA.

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CloneID	ONa32279
Clone ID Related Accession (Same CDS sequence)	XM_008849858.2
Accession Version	XM_008849858.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849858.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGTGAATGA CACTAATGTT 
CAGTTTTTGG ACCAAGATGA TGACGATGAC CCCGACACAG AGCTGTACCT CACGCAACCC 
TTTGCGTGTG GGACAGCTTT TGCCGTCAGC GTCCTGGACT CGCTCATGAG TGCGACATAC 
TTCAATGACA ATATCCTCAC CCTGATACGG ACCTTGGTGA CAGGAGGAGC CACACCAGAG 
CTCGAGGCTC TGATTGCTGA GGAGAATGCA CTTCGAGGGG GCTACAGCAC TCCCCAGACA 
CTGGCCAACA GGGACCGTTG CAGAGTGGCC CAGTTAGCTC TGTTGGATGG GCCCTTTGCA 
GACTTAGGGG ATGGTGGTTG TTATGGTGAT TTGTTCTGCA AAGCTCTGAA AACATATAAT 
ATGCTTTGTT TTGGAATTTA CCGGCTGAGA GATGCCCACC TCAGCACCCC CAGCCAGTGT 
ACGAAAAGGT ACGTCATCAC CAACCCCCCC TACGAGTTTG AGCTGGTGCC GACAGACCTG 
ATCTTCTGCC TGATGCAGTT TGACCACAAC GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT 
TCCTCCCACT CATCACAGTC CTCCAGCAAG AAGAGCTCCT CTGTCCACTC CATCCCGTCC 
ACAGCAAACC GGCCAAACCG GCCCAAGTCC AGGGAGTCCC GCGACAAACA GAATGCAACA 
AGGATGAATA GGATGGGCCA AGAAAAGAAA TGGTTTACAG ATGAACCGGA TAATGCCTAT 
CCCAGAAACA TTCAAATCAA GCCCATGAGT ACCCACATGG CTAACCAGAT CAACCAATAT 
AAATCCACAA GCAGCCTGAT CCCTCCAATC AGAGAAGTTG AAGATGAATG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848080.1
CDS	156..4049
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIYSKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_008849858.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849858.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGTGAATGA CACTAATGTT 
CAGTTTTTGG ACCAAGATGA TGACGATGAC CCCGACACAG AGCTGTACCT CACGCAACCC 
TTTGCGTGTG GGACAGCTTT TGCCGTCAGC GTCCTGGACT CGCTCATGAG TGCGACATAC 
TTCAATGACA ATATCCTCAC CCTGATACGG ACCTTGGTGA CAGGAGGAGC CACACCAGAG 
CTCGAGGCTC TGATTGCTGA GGAGAATGCA CTTCGAGGGG GCTACAGCAC TCCCCAGACA 
CTGGCCAACA GGGACCGTTG CAGAGTGGCC CAGTTAGCTC TGTTGGATGG GCCCTTTGCA 
GACTTAGGGG ATGGTGGTTG TTATGGTGAT TTGTTCTGCA AAGCTCTGAA AACATATAAT 
ATGCTTTGTT TTGGAATTTA CCGGCTGAGA GATGCCCACC TCAGCACCCC CAGCCAGTGT 
ACGAAAAGGT ACGTCATCAC CAACCCCCCC TACGAGTTTG AGCTGGTGCC GACAGACCTG 
ATCTTCTGCC TGATGCAGTT TGACCACAAC GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT 
TCCTCCCACT CATCACAGTC CTCCAGCAAG AAGAGCTCCT CTGTCCACTC CATCCCGTCC 
ACAGCAAACC GGCCAAACCG GCCCAAGTCC AGGGAGTCCC GCGACAAACA GAATGCAACA 
AGGATGAATA GGATGGGCCA AGAAAAGAAA TGGTTTACAG ATGAACCGGA TAATGCCTAT 
CCCAGAAACA TTCAAATCAA GCCCATGAGT ACCCACATGG CTAACCAGAT CAACCAATAT 
AAATCCACAA GCAGCCTGAT CCCTCCAATC AGAGAAGTTG AAGATGAATG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849859.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848081.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ONa32280
Clone ID Related Accession (Same CDS sequence)	XM_008849859.2
Accession Version	XM_008849859.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849859.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CAGTCAAGGC TGCAGCTAGA TCATGCTATC ACAAAGACCC ATTTGTGCAC 
AAGAACGCAA CTCCCAAATC TCGGCTTGTG ACTGAGCCAG TTGAAGATGA ACAGCCGTCA 
ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC GGGGGCATGA GGAACTCGCC CAACTCCTCC 
CCGAAGCTGA TGAGGCATGA CCCCTTACTA ATTCCTGGCA ATGATCAGAT CGACAACATG 
GACTCCAATG TGAAGAAATA CGACTCTACA GGGATGTTTC ACTGGTGCGC AGCCAAGGAG 
ATCGAGAAAG TCATCCTGAC TCGAAGCGAA GCTGCCATGA CTGTCCTGAG TGGCCACGTC 
GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA GCCCTGATTG GCCTCCGGAA CCTGGTGATG 
CCGCTTCGTG CCAGCAACTT CCACTACCAT GAGCTCAAAC ACATTGTGTT TGTGGGATCC 
ATTGAGTACC TCAAGCGGGA ATGGGAGACA CTTCACAACT TCCCCAAAGT GTCCATATTG 
CCGGGTACGC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGTGACATG 
TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATA CTTCGCTGCA GGACAAGGAA 
TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT CGGGGTCTTG 
CAGGCTAATT CCCAAGGATT CACACCTCCA GGAATGGACA GATCCTCTCC TGACAACAGC 
CCAGTGCACG GGATGTTACG GCAGCCATCC ATCACGACTG GGGTCAATAT CCCCATCATC 
ACAGAACTCG CTAAACCGGG CAAGTTGCCT TTGGTATCAG TCAATCAGGA AAAAAACAGT 
GGGACGCACA TTCTAATGAT AACGGAATTG GTGAATGACA CTAATGTTCA GTTTTTGGAC 
CAAGATGATG ACGATGACCC CGACACAGAG CTGTACCTCA CGCAACCCTT TGCGTGTGGG 
ACAGCTTTTG CCGTCAGCGT CCTGGACTCG CTCATGAGTG CGACATACTT CAATGACAAT 
ATCCTCACCC TGATACGGAC CTTGGTGACA GGAGGAGCCA CACCAGAGCT CGAGGCTCTG 
ATTGCTGAGG AGAATGCACT TCGAGGGGGC TACAGCACTC CCCAGACACT GGCCAACAGG 
GACCGTTGCA GAGTGGCCCA GTTAGCTCTG TTGGATGGGC CCTTTGCAGA CTTAGGGGAT 
GGTGGTTGTT ATGGTGATTT GTTCTGCAAA GCTCTGAAAA CATATAATAT GCTTTGTTTT 
GGAATTTACC GGCTGAGAGA TGCCCACCTC AGCACCCCCA GCCAGTGTAC GAAAAGGTAC 
GTCATCACCA ACCCCCCCTA CGAGTTTGAG CTGGTGCCGA CAGACCTGAT CTTCTGCCTG 
ATGCAGTTTG ACCACAACGC CGGCCAGTCC CGGGCCAGCC TGTCCCATTC CTCCCACTCA 
TCACAGTCCT CCAGCAAGAA GAGCTCCTCT GTCCACTCCA TCCCGTCCAC AGCAAACCGG 
CCAAACCGGC CCAAGTCCAG GGAGTCCCGC GACAAACAGA ATGCAACAAG GATGAATAGG 
ATGGGCCAAG CAGAAAAGAA ATGGTTTACA GATGAACCGG ATAATGCCTA TCCCAGAAAC 
ATTCAAATCA AGCCCATGAG TACCCACATG GCTAACCAGA TCAACCAATA TAAATCCACA 
AGCAGCCTGA TCCCTCCAAT CAGAGAAGTT GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848081.1
CDS	156..4040
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPVKAAARSCYHKDPFVHKNATPKSRLVTEPVEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_008849859.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849859.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CAGTCAAGGC TGCAGCTAGA TCATGCTATC ACAAAGACCC ATTTGTGCAC 
AAGAACGCAA CTCCCAAATC TCGGCTTGTG ACTGAGCCAG TTGAAGATGA ACAGCCGTCA 
ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC GGGGGCATGA GGAACTCGCC CAACTCCTCC 
CCGAAGCTGA TGAGGCATGA CCCCTTACTA ATTCCTGGCA ATGATCAGAT CGACAACATG 
GACTCCAATG TGAAGAAATA CGACTCTACA GGGATGTTTC ACTGGTGCGC AGCCAAGGAG 
ATCGAGAAAG TCATCCTGAC TCGAAGCGAA GCTGCCATGA CTGTCCTGAG TGGCCACGTC 
GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA GCCCTGATTG GCCTCCGGAA CCTGGTGATG 
CCGCTTCGTG CCAGCAACTT CCACTACCAT GAGCTCAAAC ACATTGTGTT TGTGGGATCC 
ATTGAGTACC TCAAGCGGGA ATGGGAGACA CTTCACAACT TCCCCAAAGT GTCCATATTG 
CCGGGTACGC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGTGACATG 
TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATA CTTCGCTGCA GGACAAGGAA 
TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT CGGGGTCTTG 
CAGGCTAATT CCCAAGGATT CACACCTCCA GGAATGGACA GATCCTCTCC TGACAACAGC 
CCAGTGCACG GGATGTTACG GCAGCCATCC ATCACGACTG GGGTCAATAT CCCCATCATC 
ACAGAACTCG CTAAACCGGG CAAGTTGCCT TTGGTATCAG TCAATCAGGA AAAAAACAGT 
GGGACGCACA TTCTAATGAT AACGGAATTG GTGAATGACA CTAATGTTCA GTTTTTGGAC 
CAAGATGATG ACGATGACCC CGACACAGAG CTGTACCTCA CGCAACCCTT TGCGTGTGGG 
ACAGCTTTTG CCGTCAGCGT CCTGGACTCG CTCATGAGTG CGACATACTT CAATGACAAT 
ATCCTCACCC TGATACGGAC CTTGGTGACA GGAGGAGCCA CACCAGAGCT CGAGGCTCTG 
ATTGCTGAGG AGAATGCACT TCGAGGGGGC TACAGCACTC CCCAGACACT GGCCAACAGG 
GACCGTTGCA GAGTGGCCCA GTTAGCTCTG TTGGATGGGC CCTTTGCAGA CTTAGGGGAT 
GGTGGTTGTT ATGGTGATTT GTTCTGCAAA GCTCTGAAAA CATATAATAT GCTTTGTTTT 
GGAATTTACC GGCTGAGAGA TGCCCACCTC AGCACCCCCA GCCAGTGTAC GAAAAGGTAC 
GTCATCACCA ACCCCCCCTA CGAGTTTGAG CTGGTGCCGA CAGACCTGAT CTTCTGCCTG 
ATGCAGTTTG ACCACAACGC CGGCCAGTCC CGGGCCAGCC TGTCCCATTC CTCCCACTCA 
TCACAGTCCT CCAGCAAGAA GAGCTCCTCT GTCCACTCCA TCCCGTCCAC AGCAAACCGG 
CCAAACCGGC CCAAGTCCAG GGAGTCCCGC GACAAACAGA ATGCAACAAG GATGAATAGG 
ATGGGCCAAG CAGAAAAGAA ATGGTTTACA GATGAACCGG ATAATGCCTA TCCCAGAAAC 
ATTCAAATCA AGCCCATGAG TACCCACATG GCTAACCAGA TCAACCAATA TAAATCCACA 
AGCAGCCTGA TCCCTCCAAT CAGAGAAGTT GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_017802381.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017657870.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ONa32281
Clone ID Related Accession (Same CDS sequence)	XM_017802381.1
Accession Version	XM_017802381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCCC 
AAGACGTCCA TCTACAAGAG AATGAGCCGA GCATGTTGTT TTGATTGCGG ACGTTCTGAG 
CGTGACTGCT CATGCATGTC AGGCCGTGTG CGTGGTAACG TGGACACCCC TGAGAGAACC 
TTCCCGCTTT CTTCTGTCTC TGTTAATGAT TGCGCCACCA GTTTCCGTGC CTTTGAAGAT 
GAACAGCCGT CAACGCTGTC ACCCAAAAAA AAACAGCGCA ACGGGGGCAT GAGGAACTCG 
CCCAACTCCT CCCCGAAGCT GATGAGGCAT GACCCCTTAC TAATTCCTGG CAATGATCAG 
ATCGACAACA TGGACTCCAA TGTGAAGAAA TACGACTCTA CAGGGATGTT TCACTGGTGC 
GCAGCCAAGG AGATCGAGAA AGTCATCCTG ACTCGAAGCG AAGCTGCCAT GACTGTCCTG 
AGTGGCCACG TCGTAGTCTG CATCTTTGGG GATGTCAGCT CAGCCCTGAT TGGCCTCCGG 
AACCTGGTGA TGCCGCTTCG TGCCAGCAAC TTCCACTACC ATGAGCTCAA ACACATTGTG 
TTTGTGGGAT CCATTGAGTA CCTCAAGCGG GAATGGGAGA CACTTCACAA CTTCCCCAAA 
GTGTCCATAT TGCCGGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGTGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TACTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATCGGGGTCT TGCAGGCTAA TTCCCAAGGA TTCACACCTC CAGGAATGGA CAGATCCTCT 
CCTGACAACA GCCCAGTGCA CGGGATGTTA CGGCAGCCAT CCATCACGAC TGGGGTCAAT 
ATCCCCATCA TCACAGAACT CGTGAATGAC ACTAATGTTC AGTTTTTGGA CCAAGATGAT 
GACGATGACC CCGACACAGA GCTGTACCTC ACGCAACCCT TTGCGTGTGG GACAGCTTTT 
GCCGTCAGCG TCCTGGACTC GCTCATGAGT GCGACATACT TCAATGACAA TATCCTCACC 
CTGATACGGA CCTTGGTGAC AGGAGGAGCC ACACCAGAGC TCGAGGCTCT GATTGCTGAG 
GAGAATGCAC TTCGAGGGGG CTACAGCACT CCCCAGACAC TGGCCAACAG GGACCGTTGC 
AGAGTGGCCC AGTTAGCTCT GTTGGATGGG CCCTTTGCAG ACTTAGGGGA TGGTGGTTGT 
TATGGTGATT TGTTCTGCAA AGCTCTGAAA ACATATAATA TGCTTTGTTT TGGAATTTAC 
CGGCTGAGAG ATGCCCACCT CAGCACCCCC AGCCAGTGTA CGAAAAGGTA CGTCATCACC 
AACCCCCCCT ACGAGTTTGA GCTGGTGCCG ACAGACCTGA TCTTCTGCCT GATGCAGTTT 
GACCACAACG CCGGCCAGTC CCGGGCCAGC CTGTCCCATT CCTCCCACTC ATCACAGTCC 
TCCAGCAAGA AGAGCTCCTC TGTCCACTCC ATCCCGTCCA CAGCAAACCG GCCAAACCGG 
CCCAAGTCCA GGGAGTCCCG CGACAAACAG AATGCAACAA GGATGAATAG GATGGGCCAA 
GAAAAGAAAT GGTTTACAGA TGAACCGGAT AATGCCTATC CCAGAAACAT TCAAATCAAG 
CCCATGAGTA CCCACATGGC TAACCAGATC AACCAATATA AATCCACAAG CAGCCTGATC 
CCTCCAATCA GAGAAGTTGA AGATGAATGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017657870.1
CDS	156..4028
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017802381.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017802381.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCCC 
AAGACGTCCA TCTACAAGAG AATGAGCCGA GCATGTTGTT TTGATTGCGG ACGTTCTGAG 
CGTGACTGCT CATGCATGTC AGGCCGTGTG CGTGGTAACG TGGACACCCC TGAGAGAACC 
TTCCCGCTTT CTTCTGTCTC TGTTAATGAT TGCGCCACCA GTTTCCGTGC CTTTGAAGAT 
GAACAGCCGT CAACGCTGTC ACCCAAAAAA AAACAGCGCA ACGGGGGCAT GAGGAACTCG 
CCCAACTCCT CCCCGAAGCT GATGAGGCAT GACCCCTTAC TAATTCCTGG CAATGATCAG 
ATCGACAACA TGGACTCCAA TGTGAAGAAA TACGACTCTA CAGGGATGTT TCACTGGTGC 
GCAGCCAAGG AGATCGAGAA AGTCATCCTG ACTCGAAGCG AAGCTGCCAT GACTGTCCTG 
AGTGGCCACG TCGTAGTCTG CATCTTTGGG GATGTCAGCT CAGCCCTGAT TGGCCTCCGG 
AACCTGGTGA TGCCGCTTCG TGCCAGCAAC TTCCACTACC ATGAGCTCAA ACACATTGTG 
TTTGTGGGAT CCATTGAGTA CCTCAAGCGG GAATGGGAGA CACTTCACAA CTTCCCCAAA 
GTGTCCATAT TGCCGGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGTGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TACTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATCGGGGTCT TGCAGGCTAA TTCCCAAGGA TTCACACCTC CAGGAATGGA CAGATCCTCT 
CCTGACAACA GCCCAGTGCA CGGGATGTTA CGGCAGCCAT CCATCACGAC TGGGGTCAAT 
ATCCCCATCA TCACAGAACT CGTGAATGAC ACTAATGTTC AGTTTTTGGA CCAAGATGAT 
GACGATGACC CCGACACAGA GCTGTACCTC ACGCAACCCT TTGCGTGTGG GACAGCTTTT 
GCCGTCAGCG TCCTGGACTC GCTCATGAGT GCGACATACT TCAATGACAA TATCCTCACC 
CTGATACGGA CCTTGGTGAC AGGAGGAGCC ACACCAGAGC TCGAGGCTCT GATTGCTGAG 
GAGAATGCAC TTCGAGGGGG CTACAGCACT CCCCAGACAC TGGCCAACAG GGACCGTTGC 
AGAGTGGCCC AGTTAGCTCT GTTGGATGGG CCCTTTGCAG ACTTAGGGGA TGGTGGTTGT 
TATGGTGATT TGTTCTGCAA AGCTCTGAAA ACATATAATA TGCTTTGTTT TGGAATTTAC 
CGGCTGAGAG ATGCCCACCT CAGCACCCCC AGCCAGTGTA CGAAAAGGTA CGTCATCACC 
AACCCCCCCT ACGAGTTTGA GCTGGTGCCG ACAGACCTGA TCTTCTGCCT GATGCAGTTT 
GACCACAACG CCGGCCAGTC CCGGGCCAGC CTGTCCCATT CCTCCCACTC ATCACAGTCC 
TCCAGCAAGA AGAGCTCCTC TGTCCACTCC ATCCCGTCCA CAGCAAACCG GCCAAACCGG 
CCCAAGTCCA GGGAGTCCCG CGACAAACAG AATGCAACAA GGATGAATAG GATGGGCCAA 
GAAAAGAAAT GGTTTACAGA TGAACCGGAT AATGCCTATC CCAGAAACAT TCAAATCAAG 
CCCATGAGTA CCCACATGGC TAACCAGATC AACCAATATA AATCCACAAG CAGCCTGATC 
CCTCCAATCA GAGAAGTTGA AGATGAATGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849861.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848083.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X9, mRNA.

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CloneID	ONa32282
Clone ID Related Accession (Same CDS sequence)	XM_008849861.2
Accession Version	XM_008849861.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGCTAAACC GGGCAAGTTG 
CCTTTGGTAT CAGTCAATCA GGAAAAAAAC AGTGGGACGC ACATTCTAAT GATAACGGAA 
TTGGTGAATG ACACTAATGT TCAGTTTTTG GACCAAGATG ATGACGATGA CCCCGACACA 
GAGCTGTACC TCACGCAACC CTTTGCGTGT GGGACAGCTT TTGCCGTCAG CGTCCTGGAC 
TCGCTCATGA GTGCGACATA CTTCAATGAC AATATCCTCA CCCTGATACG GACCTTGGTG 
ACAGGAGGAG CCACACCAGA GCTCGAGGCT CTGATTGCTG AGGAGAATGC ACTTCGAGGG 
GGCTACAGCA CTCCCCAGAC ACTGGCCAAC AGGGACCGTT GCAGAGTGGC CCAGTTAGCT 
CTGTTGGATG GGCCCTTTGC AGACTTAGGG GATGGTGGTT GTTATGGTGA TTTGTTCTGC 
AAAGCTCTGA AAACATATAA TATGCTTTGT TTTGGAATTT ACCGGCTGAG AGATGCCCAC 
CTCAGCACCC CCAGCCAGTG TACGAAAAGG TACGTCATCA CCAACCCCCC CTACGAGTTT 
GAGCTGGTGC CGACAGACCT GATCTTCTGC CTGATGCAGT TTGACCACAA CGCCGGCCAG 
TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC TCATCACAGT CCTCCAGCAA GAAGAGCTCC 
TCTGTCCACT CCATCCCGTC CACAGCAAAC CGGCCAAACC GGCCCAAGTC CAGGGAGTCC 
CGCGACAAAC AGAACAGAAA AGAAATGGTT TACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848083.1
CDS	156..3974
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIYSKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_008849861.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849861.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGCTAAACC GGGCAAGTTG 
CCTTTGGTAT CAGTCAATCA GGAAAAAAAC AGTGGGACGC ACATTCTAAT GATAACGGAA 
TTGGTGAATG ACACTAATGT TCAGTTTTTG GACCAAGATG ATGACGATGA CCCCGACACA 
GAGCTGTACC TCACGCAACC CTTTGCGTGT GGGACAGCTT TTGCCGTCAG CGTCCTGGAC 
TCGCTCATGA GTGCGACATA CTTCAATGAC AATATCCTCA CCCTGATACG GACCTTGGTG 
ACAGGAGGAG CCACACCAGA GCTCGAGGCT CTGATTGCTG AGGAGAATGC ACTTCGAGGG 
GGCTACAGCA CTCCCCAGAC ACTGGCCAAC AGGGACCGTT GCAGAGTGGC CCAGTTAGCT 
CTGTTGGATG GGCCCTTTGC AGACTTAGGG GATGGTGGTT GTTATGGTGA TTTGTTCTGC 
AAAGCTCTGA AAACATATAA TATGCTTTGT TTTGGAATTT ACCGGCTGAG AGATGCCCAC 
CTCAGCACCC CCAGCCAGTG TACGAAAAGG TACGTCATCA CCAACCCCCC CTACGAGTTT 
GAGCTGGTGC CGACAGACCT GATCTTCTGC CTGATGCAGT TTGACCACAA CGCCGGCCAG 
TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC TCATCACAGT CCTCCAGCAA GAAGAGCTCC 
TCTGTCCACT CCATCCCGTC CACAGCAAAC CGGCCAAACC GGCCCAAGTC CAGGGAGTCC 
CGCGACAAAC AGAACAGAAA AGAAATGGTT TACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849863.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848085.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ONa32283
Clone ID Related Accession (Same CDS sequence)	XM_008849863.2
Accession Version	XM_008849863.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849863.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGCTAAACC GGGCAAGTTG 
CCTTTGGTAT CAGTCAATCA GGAAAAAAAC AGTGGGACGC ACATTCTAAT GATAACGGAA 
TTGGTGAATG ACACTAATGT TCAGTTTTTG GACCAAGATG ATGACGATGA CCCCGACACA 
GAGCTGTACC TCACGCAACC CTTTGCGTGT GGGACAGCTT TTGCCGTCAG CGTCCTGGAC 
TCGCTCATGA GTGCGACATA CTTCAATGAC AATATCCTCA CCCTGATACG GACCTTGGTG 
ACAGGAGGAG CCACACCAGA GCTCGAGGCT CTGATTGCTG AGGAGAATGC ACTTCGAGGG 
GGCTACAGCA CTCCCCAGAC ACTGGCCAAC AGGGACCGTT GCAGAGTGGC CCAGTTAGCT 
CTGTTGGATG GGCCCTTTGC AGACTTAGGG GATGGTGGTT GTTATGGTGA TTTGTTCTGC 
AAAGCTCTGA AAACATATAA TATGCTTTGT TTTGGAATTT ACCGGCTGAG AGATGCCCAC 
CTCAGCACCC CCAGCCAGTG TACGAAAAGG TACGTCATCA CCAACCCCCC CTACGAGTTT 
GAGCTGGTGC CGACAGACCT GATCTTCTGC CTGATGCAGT TTGACCACAA CGCCGGCCAG 
TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC TCATCACAGT CCTCCAGCAA GAAGAGCTCC 
TCTGTCCACT CCATCCCGTC CACAGCAAAC CGGCCAAACC GGCCCAAGTC CAGGGAGTCC 
CGCGACAAAC AGAAAAAAGA AATGGTTTAC AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848085.1
CDS	156..3971
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIYSKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR

Target ORF information:

RefSeq Version	XM_008849863.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849863.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGCTAAACC GGGCAAGTTG 
CCTTTGGTAT CAGTCAATCA GGAAAAAAAC AGTGGGACGC ACATTCTAAT GATAACGGAA 
TTGGTGAATG ACACTAATGT TCAGTTTTTG GACCAAGATG ATGACGATGA CCCCGACACA 
GAGCTGTACC TCACGCAACC CTTTGCGTGT GGGACAGCTT TTGCCGTCAG CGTCCTGGAC 
TCGCTCATGA GTGCGACATA CTTCAATGAC AATATCCTCA CCCTGATACG GACCTTGGTG 
ACAGGAGGAG CCACACCAGA GCTCGAGGCT CTGATTGCTG AGGAGAATGC ACTTCGAGGG 
GGCTACAGCA CTCCCCAGAC ACTGGCCAAC AGGGACCGTT GCAGAGTGGC CCAGTTAGCT 
CTGTTGGATG GGCCCTTTGC AGACTTAGGG GATGGTGGTT GTTATGGTGA TTTGTTCTGC 
AAAGCTCTGA AAACATATAA TATGCTTTGT TTTGGAATTT ACCGGCTGAG AGATGCCCAC 
CTCAGCACCC CCAGCCAGTG TACGAAAAGG TACGTCATCA CCAACCCCCC CTACGAGTTT 
GAGCTGGTGC CGACAGACCT GATCTTCTGC CTGATGCAGT TTGACCACAA CGCCGGCCAG 
TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC TCATCACAGT CCTCCAGCAA GAAGAGCTCC 
TCTGTCCACT CCATCCCGTC CACAGCAAAC CGGCCAAACC GGCCCAAGTC CAGGGAGTCC 
CGCGACAAAC AGAAAAAAGA AATGGTTTAC AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849864.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848086.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ONa32284
Clone ID Related Accession (Same CDS sequence)	XM_008849864.2
Accession Version	XM_008849864.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849864.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TTGAAGATGA ACAGCCGTCA ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC 
GGGGGCATGA GGAACTCGCC CAACTCCTCC CCGAAGCTGA TGAGGCATGA CCCCTTACTA 
ATTCCTGGCA ATGATCAGAT CGACAACATG GACTCCAATG TGAAGAAATA CGACTCTACA 
GGGATGTTTC ACTGGTGCGC AGCCAAGGAG ATCGAGAAAG TCATCCTGAC TCGAAGCGAA 
GCTGCCATGA CTGTCCTGAG TGGCCACGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA 
GCCCTGATTG GCCTCCGGAA CCTGGTGATG CCGCTTCGTG CCAGCAACTT CCACTACCAT 
GAGCTCAAAC ACATTGTGTT TGTGGGATCC ATTGAGTACC TCAAGCGGGA ATGGGAGACA 
CTTCACAACT TCCCCAAAGT GTCCATATTG CCGGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCA 
GGAATGGACA GATCCTCTCC TGACAACAGC CCAGTGCACG GGATGTTACG GCAGCCATCC 
ATCACGACTG GGGTCAATAT CCCCATCATC ACAGAACTCG CTAAACCGGG CAAGTTGCCT 
TTGGTATCAG TCAATCAGGA AAAAAACAGT GGGACGCACA TTCTAATGAT AACGGAATTG 
GTGAATGACA CTAATGTTCA GTTTTTGGAC CAAGATGATG ACGATGACCC CGACACAGAG 
CTGTACCTCA CGCAACCCTT TGCGTGTGGG ACAGCTTTTG CCGTCAGCGT CCTGGACTCG 
CTCATGAGTG CGACATACTT CAATGACAAT ATCCTCACCC TGATACGGAC CTTGGTGACA 
GGAGGAGCCA CACCAGAGCT CGAGGCTCTG ATTGCTGAGG AGAATGCACT TCGAGGGGGC 
TACAGCACTC CCCAGACACT GGCCAACAGG GACCGTTGCA GAGTGGCCCA GTTAGCTCTG 
TTGGATGGGC CCTTTGCAGA CTTAGGGGAT GGTGGTTGTT ATGGTGATTT GTTCTGCAAA 
GCTCTGAAAA CATATAATAT GCTTTGTTTT GGAATTTACC GGCTGAGAGA TGCCCACCTC 
AGCACCCCCA GCCAGTGTAC GAAAAGGTAC GTCATCACCA ACCCCCCCTA CGAGTTTGAG 
CTGGTGCCGA CAGACCTGAT CTTCTGCCTG ATGCAGTTTG ACCACAACGC CGGCCAGTCC 
CGGGCCAGCC TGTCCCATTC CTCCCACTCA TCACAGTCCT CCAGCAAGAA GAGCTCCTCT 
GTCCACTCCA TCCCGTCCAC AGCAAACCGG CCAAACCGGC CCAAGTCCAG GGAGTCCCGC 
GACAAACAGA ATGCAACAAG GATGAATAGG ATGGGCCAAG CAGAAAAGAA ATGGTTTACA 
GATGAACCGG ATAATGCCTA TCCCAGAAAC ATTCAAATCA AGCCCATGAG TACCCACATG 
GCTAACCAGA TCAACCAATA TAAATCCACA AGCAGCCTGA TCCCTCCAAT CAGAGAAGTT 
GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848086.1
CDS	156..3950
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_008849864.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849864.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TTGAAGATGA ACAGCCGTCA ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC 
GGGGGCATGA GGAACTCGCC CAACTCCTCC CCGAAGCTGA TGAGGCATGA CCCCTTACTA 
ATTCCTGGCA ATGATCAGAT CGACAACATG GACTCCAATG TGAAGAAATA CGACTCTACA 
GGGATGTTTC ACTGGTGCGC AGCCAAGGAG ATCGAGAAAG TCATCCTGAC TCGAAGCGAA 
GCTGCCATGA CTGTCCTGAG TGGCCACGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA 
GCCCTGATTG GCCTCCGGAA CCTGGTGATG CCGCTTCGTG CCAGCAACTT CCACTACCAT 
GAGCTCAAAC ACATTGTGTT TGTGGGATCC ATTGAGTACC TCAAGCGGGA ATGGGAGACA 
CTTCACAACT TCCCCAAAGT GTCCATATTG CCGGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCA 
GGAATGGACA GATCCTCTCC TGACAACAGC CCAGTGCACG GGATGTTACG GCAGCCATCC 
ATCACGACTG GGGTCAATAT CCCCATCATC ACAGAACTCG CTAAACCGGG CAAGTTGCCT 
TTGGTATCAG TCAATCAGGA AAAAAACAGT GGGACGCACA TTCTAATGAT AACGGAATTG 
GTGAATGACA CTAATGTTCA GTTTTTGGAC CAAGATGATG ACGATGACCC CGACACAGAG 
CTGTACCTCA CGCAACCCTT TGCGTGTGGG ACAGCTTTTG CCGTCAGCGT CCTGGACTCG 
CTCATGAGTG CGACATACTT CAATGACAAT ATCCTCACCC TGATACGGAC CTTGGTGACA 
GGAGGAGCCA CACCAGAGCT CGAGGCTCTG ATTGCTGAGG AGAATGCACT TCGAGGGGGC 
TACAGCACTC CCCAGACACT GGCCAACAGG GACCGTTGCA GAGTGGCCCA GTTAGCTCTG 
TTGGATGGGC CCTTTGCAGA CTTAGGGGAT GGTGGTTGTT ATGGTGATTT GTTCTGCAAA 
GCTCTGAAAA CATATAATAT GCTTTGTTTT GGAATTTACC GGCTGAGAGA TGCCCACCTC 
AGCACCCCCA GCCAGTGTAC GAAAAGGTAC GTCATCACCA ACCCCCCCTA CGAGTTTGAG 
CTGGTGCCGA CAGACCTGAT CTTCTGCCTG ATGCAGTTTG ACCACAACGC CGGCCAGTCC 
CGGGCCAGCC TGTCCCATTC CTCCCACTCA TCACAGTCCT CCAGCAAGAA GAGCTCCTCT 
GTCCACTCCA TCCCGTCCAC AGCAAACCGG CCAAACCGGC CCAAGTCCAG GGAGTCCCGC 
GACAAACAGA ATGCAACAAG GATGAATAGG ATGGGCCAAG CAGAAAAGAA ATGGTTTACA 
GATGAACCGG ATAATGCCTA TCCCAGAAAC ATTCAAATCA AGCCCATGAG TACCCACATG 
GCTAACCAGA TCAACCAATA TAAATCCACA AGCAGCCTGA TCCCTCCAAT CAGAGAAGTT 
GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849865.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848087.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X9, mRNA.

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CloneID	ONa32285
Clone ID Related Accession (Same CDS sequence)	XM_008849865.2
Accession Version	XM_008849865.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTT 
GAAGATGAAC AGCCGTCAAC GCTGTCACCC AAAAAAAAAC AGCGCAACGG GGGCATGAGG 
AACTCGCCCA ACTCCTCCCC GAAGCTGATG AGGCATGACC CCTTACTAAT TCCTGGCAAT 
GATCAGATCG ACAACATGGA CTCCAATGTG AAGAAATACG ACTCTACAGG GATGTTTCAC 
TGGTGCGCAG CCAAGGAGAT CGAGAAAGTC ATCCTGACTC GAAGCGAAGC TGCCATGACT 
GTCCTGAGTG GCCACGTCGT AGTCTGCATC TTTGGGGATG TCAGCTCAGC CCTGATTGGC 
CTCCGGAACC TGGTGATGCC GCTTCGTGCC AGCAACTTCC ACTACCATGA GCTCAAACAC 
ATTGTGTTTG TGGGATCCAT TGAGTACCTC AAGCGGGAAT GGGAGACACT TCACAACTTC 
CCCAAAGTGT CCATATTGCC GGGTACGCCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATACT 
TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA CACCTCCAGG AATGGACAGA 
TCCTCTCCTG ACAACAGCCC AGTGCACGGG ATGTTACGGC AGCCATCCAT CACGACTGGG 
GTCAATATCC CCATCATCAC AGAACTCGCT AAACCGGGCA AGTTGCCTTT GGTATCAGTC 
AATCAGGAAA AAAACAGTGG GACGCACATT CTAATGATAA CGGAATTGGT GAATGACACT 
AATGTTCAGT TTTTGGACCA AGATGATGAC GATGACCCCG ACACAGAGCT GTACCTCACG 
CAACCCTTTG CGTGTGGGAC AGCTTTTGCC GTCAGCGTCC TGGACTCGCT CATGAGTGCG 
ACATACTTCA ATGACAATAT CCTCACCCTG ATACGGACCT TGGTGACAGG AGGAGCCACA 
CCAGAGCTCG AGGCTCTGAT TGCTGAGGAG AATGCACTTC GAGGGGGCTA CAGCACTCCC 
CAGACACTGG CCAACAGGGA CCGTTGCAGA GTGGCCCAGT TAGCTCTGTT GGATGGGCCC 
TTTGCAGACT TAGGGGATGG TGGTTGTTAT GGTGATTTGT TCTGCAAAGC TCTGAAAACA 
TATAATATGC TTTGTTTTGG AATTTACCGG CTGAGAGATG CCCACCTCAG CACCCCCAGC 
CAGTGTACGA AAAGGTACGT CATCACCAAC CCCCCCTACG AGTTTGAGCT GGTGCCGACA 
GACCTGATCT TCTGCCTGAT GCAGTTTGAC CACAACGCCG GCCAGTCCCG GGCCAGCCTG 
TCCCATTCCT CCCACTCATC ACAGTCCTCC AGCAAGAAGA GCTCCTCTGT CCACTCCATC 
CCGTCCACAG CAAACCGGCC AAACCGGCCC AAGTCCAGGG AGTCCCGCGA CAAACAGAAT 
GCAACAAGGA TGAATAGGAT GGGCCAAGCA GAAAAGAAAT GGTTTACAGA TGAACCGGAT 
AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGTA CCCACATGGC TAACCAGATC 
AACCAATATA AATCCACAAG CAGCCTGATC CCTCCAATCA GAGAAGTTGA AGATGAATGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848087.1
CDS	156..3938
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_008849865.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849865.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTT 
GAAGATGAAC AGCCGTCAAC GCTGTCACCC AAAAAAAAAC AGCGCAACGG GGGCATGAGG 
AACTCGCCCA ACTCCTCCCC GAAGCTGATG AGGCATGACC CCTTACTAAT TCCTGGCAAT 
GATCAGATCG ACAACATGGA CTCCAATGTG AAGAAATACG ACTCTACAGG GATGTTTCAC 
TGGTGCGCAG CCAAGGAGAT CGAGAAAGTC ATCCTGACTC GAAGCGAAGC TGCCATGACT 
GTCCTGAGTG GCCACGTCGT AGTCTGCATC TTTGGGGATG TCAGCTCAGC CCTGATTGGC 
CTCCGGAACC TGGTGATGCC GCTTCGTGCC AGCAACTTCC ACTACCATGA GCTCAAACAC 
ATTGTGTTTG TGGGATCCAT TGAGTACCTC AAGCGGGAAT GGGAGACACT TCACAACTTC 
CCCAAAGTGT CCATATTGCC GGGTACGCCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATACT 
TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA CACCTCCAGG AATGGACAGA 
TCCTCTCCTG ACAACAGCCC AGTGCACGGG ATGTTACGGC AGCCATCCAT CACGACTGGG 
GTCAATATCC CCATCATCAC AGAACTCGCT AAACCGGGCA AGTTGCCTTT GGTATCAGTC 
AATCAGGAAA AAAACAGTGG GACGCACATT CTAATGATAA CGGAATTGGT GAATGACACT 
AATGTTCAGT TTTTGGACCA AGATGATGAC GATGACCCCG ACACAGAGCT GTACCTCACG 
CAACCCTTTG CGTGTGGGAC AGCTTTTGCC GTCAGCGTCC TGGACTCGCT CATGAGTGCG 
ACATACTTCA ATGACAATAT CCTCACCCTG ATACGGACCT TGGTGACAGG AGGAGCCACA 
CCAGAGCTCG AGGCTCTGAT TGCTGAGGAG AATGCACTTC GAGGGGGCTA CAGCACTCCC 
CAGACACTGG CCAACAGGGA CCGTTGCAGA GTGGCCCAGT TAGCTCTGTT GGATGGGCCC 
TTTGCAGACT TAGGGGATGG TGGTTGTTAT GGTGATTTGT TCTGCAAAGC TCTGAAAACA 
TATAATATGC TTTGTTTTGG AATTTACCGG CTGAGAGATG CCCACCTCAG CACCCCCAGC 
CAGTGTACGA AAAGGTACGT CATCACCAAC CCCCCCTACG AGTTTGAGCT GGTGCCGACA 
GACCTGATCT TCTGCCTGAT GCAGTTTGAC CACAACGCCG GCCAGTCCCG GGCCAGCCTG 
TCCCATTCCT CCCACTCATC ACAGTCCTCC AGCAAGAAGA GCTCCTCTGT CCACTCCATC 
CCGTCCACAG CAAACCGGCC AAACCGGCCC AAGTCCAGGG AGTCCCGCGA CAAACAGAAT 
GCAACAAGGA TGAATAGGAT GGGCCAAGCA GAAAAGAAAT GGTTTACAGA TGAACCGGAT 
AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGTA CCCACATGGC TAACCAGATC 
AACCAATATA AATCCACAAG CAGCCTGATC CCTCCAATCA GAGAAGTTGA AGATGAATGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849866.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848088.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ONa32286
Clone ID Related Accession (Same CDS sequence)	XM_008849866.2
Accession Version	XM_008849866.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849866.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTT 
GAAGATGAAC AGCCGTCAAC GCTGTCACCC AAAAAAAAAC AGCGCAACGG GGGCATGAGG 
AACTCGCCCA ACTCCTCCCC GAAGCTGATG AGGCATGACC CCTTACTAAT TCCTGGCAAT 
GATCAGATCG ACAACATGGA CTCCAATGTG AAGAAATACG ACTCTACAGG GATGTTTCAC 
TGGTGCGCAG CCAAGGAGAT CGAGAAAGTC ATCCTGACTC GAAGCGAAGC TGCCATGACT 
GTCCTGAGTG GCCACGTCGT AGTCTGCATC TTTGGGGATG TCAGCTCAGC CCTGATTGGC 
CTCCGGAACC TGGTGATGCC GCTTCGTGCC AGCAACTTCC ACTACCATGA GCTCAAACAC 
ATTGTGTTTG TGGGATCCAT TGAGTACCTC AAGCGGGAAT GGGAGACACT TCACAACTTC 
CCCAAAGTGT CCATATTGCC GGGTACGCCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATACT 
TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA CACCTCCAGG AATGGACAGA 
TCCTCTCCTG ACAACAGCCC AGTGCACGGG ATGTTACGGC AGCCATCCAT CACGACTGGG 
GTCAATATCC CCATCATCAC AGAACTCGCT AAACCGGGCA AGTTGCCTTT GGTATCAGTC 
AATCAGGAAA AAAACAGTGG GACGCACATT CTAATGATAA CGGAATTGGT GAATGACACT 
AATGTTCAGT TTTTGGACCA AGATGATGAC GATGACCCCG ACACAGAGCT GTACCTCACG 
CAACCCTTTG CGTGTGGGAC AGCTTTTGCC GTCAGCGTCC TGGACTCGCT CATGAGTGCG 
ACATACTTCA ATGACAATAT CCTCACCCTG ATACGGACCT TGGTGACAGG AGGAGCCACA 
CCAGAGCTCG AGGCTCTGAT TGCTGAGGAG AATGCACTTC GAGGGGGCTA CAGCACTCCC 
CAGACACTGG CCAACAGGGA CCGTTGCAGA GTGGCCCAGT TAGCTCTGTT GGATGGGCCC 
TTTGCAGACT TAGGGGATGG TGGTTGTTAT GGTGATTTGT TCTGCAAAGC TCTGAAAACA 
TATAATATGC TTTGTTTTGG AATTTACCGG CTGAGAGATG CCCACCTCAG CACCCCCAGC 
CAGTGTACGA AAAGGTACGT CATCACCAAC CCCCCCTACG AGTTTGAGCT GGTGCCGACA 
GACCTGATCT TCTGCCTGAT GCAGTTTGAC CACAACGCCG GCCAGTCCCG GGCCAGCCTG 
TCCCATTCCT CCCACTCATC ACAGTCCTCC AGCAAGAAGA GCTCCTCTGT CCACTCCATC 
CCGTCCACAG CAAACCGGCC AAACCGGCCC AAGTCCAGGG AGTCCCGCGA CAAACAGAAT 
GCAACAAGGA TGAATAGGAT GGGCCAAGAA AAGAAATGGT TTACAGATGA ACCGGATAAT 
GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGTACCC ACATGGCTAA CCAGATCAAC 
CAATATAAAT CCACAAGCAG CCTGATCCCT CCAATCAGAG AAGTTGAAGA TGAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848088.1
CDS	156..3935
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_008849866.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849866.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTT 
GAAGATGAAC AGCCGTCAAC GCTGTCACCC AAAAAAAAAC AGCGCAACGG GGGCATGAGG 
AACTCGCCCA ACTCCTCCCC GAAGCTGATG AGGCATGACC CCTTACTAAT TCCTGGCAAT 
GATCAGATCG ACAACATGGA CTCCAATGTG AAGAAATACG ACTCTACAGG GATGTTTCAC 
TGGTGCGCAG CCAAGGAGAT CGAGAAAGTC ATCCTGACTC GAAGCGAAGC TGCCATGACT 
GTCCTGAGTG GCCACGTCGT AGTCTGCATC TTTGGGGATG TCAGCTCAGC CCTGATTGGC 
CTCCGGAACC TGGTGATGCC GCTTCGTGCC AGCAACTTCC ACTACCATGA GCTCAAACAC 
ATTGTGTTTG TGGGATCCAT TGAGTACCTC AAGCGGGAAT GGGAGACACT TCACAACTTC 
CCCAAAGTGT CCATATTGCC GGGTACGCCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATACT 
TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA CACCTCCAGG AATGGACAGA 
TCCTCTCCTG ACAACAGCCC AGTGCACGGG ATGTTACGGC AGCCATCCAT CACGACTGGG 
GTCAATATCC CCATCATCAC AGAACTCGCT AAACCGGGCA AGTTGCCTTT GGTATCAGTC 
AATCAGGAAA AAAACAGTGG GACGCACATT CTAATGATAA CGGAATTGGT GAATGACACT 
AATGTTCAGT TTTTGGACCA AGATGATGAC GATGACCCCG ACACAGAGCT GTACCTCACG 
CAACCCTTTG CGTGTGGGAC AGCTTTTGCC GTCAGCGTCC TGGACTCGCT CATGAGTGCG 
ACATACTTCA ATGACAATAT CCTCACCCTG ATACGGACCT TGGTGACAGG AGGAGCCACA 
CCAGAGCTCG AGGCTCTGAT TGCTGAGGAG AATGCACTTC GAGGGGGCTA CAGCACTCCC 
CAGACACTGG CCAACAGGGA CCGTTGCAGA GTGGCCCAGT TAGCTCTGTT GGATGGGCCC 
TTTGCAGACT TAGGGGATGG TGGTTGTTAT GGTGATTTGT TCTGCAAAGC TCTGAAAACA 
TATAATATGC TTTGTTTTGG AATTTACCGG CTGAGAGATG CCCACCTCAG CACCCCCAGC 
CAGTGTACGA AAAGGTACGT CATCACCAAC CCCCCCTACG AGTTTGAGCT GGTGCCGACA 
GACCTGATCT TCTGCCTGAT GCAGTTTGAC CACAACGCCG GCCAGTCCCG GGCCAGCCTG 
TCCCATTCCT CCCACTCATC ACAGTCCTCC AGCAAGAAGA GCTCCTCTGT CCACTCCATC 
CCGTCCACAG CAAACCGGCC AAACCGGCCC AAGTCCAGGG AGTCCCGCGA CAAACAGAAT 
GCAACAAGGA TGAATAGGAT GGGCCAAGAA AAGAAATGGT TTACAGATGA ACCGGATAAT 
GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGTACCC ACATGGCTAA CCAGATCAAC 
CAATATAAAT CCACAAGCAG CCTGATCCCT CCAATCAGAG AAGTTGAAGA TGAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849867.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848089.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ONa32287
Clone ID Related Accession (Same CDS sequence)	XM_008849867.2
Accession Version	XM_008849867.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGTGAATGA CACTAATGTT 
CAGTTTTTGG ACCAAGATGA TGACGATGAC CCCGACACAG AGCTGTACCT CACGCAACCC 
TTTGCGTGTG GGACAGCTTT TGCCGTCAGC GTCCTGGACT CGCTCATGAG TGCGACATAC 
TTCAATGACA ATATCCTCAC CCTGATACGG ACCTTGGTGA CAGGAGGAGC CACACCAGAG 
CTCGAGGCTC TGATTGCTGA GGAGAATGCA CTTCGAGGGG GCTACAGCAC TCCCCAGACA 
CTGGCCAACA GGGACCGTTG CAGAGTGGCC CAGTTAGCTC TGTTGGATGG GCCCTTTGCA 
GACTTAGGGG ATGGTGGTTG TTATGGTGAT TTGTTCTGCA AAGCTCTGAA AACATATAAT 
ATGCTTTGTT TTGGAATTTA CCGGCTGAGA GATGCCCACC TCAGCACCCC CAGCCAGTGT 
ACGAAAAGGT ACGTCATCAC CAACCCCCCC TACGAGTTTG AGCTGGTGCC GACAGACCTG 
ATCTTCTGCC TGATGCAGTT TGACCACAAC GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT 
TCCTCCCACT CATCACAGTC CTCCAGCAAG AAGAGCTCCT CTGTCCACTC CATCCCGTCC 
ACAGCAAACC GGCCAAACCG GCCCAAGTCC AGGGAGTCCC GCGACAAACA GAACAGAAAA 
GAAATGGTTT ACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848089.1
CDS	156..3893
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIYSKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_008849867.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849867.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGTGAATGA CACTAATGTT 
CAGTTTTTGG ACCAAGATGA TGACGATGAC CCCGACACAG AGCTGTACCT CACGCAACCC 
TTTGCGTGTG GGACAGCTTT TGCCGTCAGC GTCCTGGACT CGCTCATGAG TGCGACATAC 
TTCAATGACA ATATCCTCAC CCTGATACGG ACCTTGGTGA CAGGAGGAGC CACACCAGAG 
CTCGAGGCTC TGATTGCTGA GGAGAATGCA CTTCGAGGGG GCTACAGCAC TCCCCAGACA 
CTGGCCAACA GGGACCGTTG CAGAGTGGCC CAGTTAGCTC TGTTGGATGG GCCCTTTGCA 
GACTTAGGGG ATGGTGGTTG TTATGGTGAT TTGTTCTGCA AAGCTCTGAA AACATATAAT 
ATGCTTTGTT TTGGAATTTA CCGGCTGAGA GATGCCCACC TCAGCACCCC CAGCCAGTGT 
ACGAAAAGGT ACGTCATCAC CAACCCCCCC TACGAGTTTG AGCTGGTGCC GACAGACCTG 
ATCTTCTGCC TGATGCAGTT TGACCACAAC GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT 
TCCTCCCACT CATCACAGTC CTCCAGCAAG AAGAGCTCCT CTGTCCACTC CATCCCGTCC 
ACAGCAAACC GGCCAAACCG GCCCAAGTCC AGGGAGTCCC GCGACAAACA GAACAGAAAA 
GAAATGGTTT ACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849870.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848092.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X15, mRNA.

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CloneID	ONa32288
Clone ID Related Accession (Same CDS sequence)	XM_008849870.2
Accession Version	XM_008849870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGTGAATGA CACTAATGTT 
CAGTTTTTGG ACCAAGATGA TGACGATGAC CCCGACACAG AGCTGTACCT CACGCAACCC 
TTTGCGTGTG GGACAGCTTT TGCCGTCAGC GTCCTGGACT CGCTCATGAG TGCGACATAC 
TTCAATGACA ATATCCTCAC CCTGATACGG ACCTTGGTGA CAGGAGGAGC CACACCAGAG 
CTCGAGGCTC TGATTGCTGA GGAGAATGCA CTTCGAGGGG GCTACAGCAC TCCCCAGACA 
CTGGCCAACA GGGACCGTTG CAGAGTGGCC CAGTTAGCTC TGTTGGATGG GCCCTTTGCA 
GACTTAGGGG ATGGTGGTTG TTATGGTGAT TTGTTCTGCA AAGCTCTGAA AACATATAAT 
ATGCTTTGTT TTGGAATTTA CCGGCTGAGA GATGCCCACC TCAGCACCCC CAGCCAGTGT 
ACGAAAAGGT ACGTCATCAC CAACCCCCCC TACGAGTTTG AGCTGGTGCC GACAGACCTG 
ATCTTCTGCC TGATGCAGTT TGACCACAAC GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT 
TCCTCCCACT CATCACAGTC CTCCAGCAAG AAGAGCTCCT CTGTCCACTC CATCCCGTCC 
ACAGCAAACC GGCCAAACCG GCCCAAGTCC AGGGAGTCCC GCGACAAACA GAAAAAAGAA 
ATGGTTTACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848092.1
CDS	156..3890
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIYSKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR

Target ORF information:

RefSeq Version	XM_008849870.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849870.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TGATCTATTC CAAGACGTCC ATCTACAAGA GAATGAGCCG AGCATGTTGT 
TTTGATTGCG GACGTTCTGA GCGTGACTGC TCATGCATGT CAGGCCGTGT GCGTGGTAAC 
GTGGACACCC CTGAGAGAAC CTTCCCGCTT TCTTCTGTCT CTGTTAATGA TTGCGCCACC 
AGTTTCCGTG CCTTTGAAGA TGAACAGCCG TCAACGCTGT CACCCAAAAA AAAACAGCGC 
AACGGGGGCA TGAGGAACTC GCCCAACTCC TCCCCGAAGC TGATGAGGCA TGACCCCTTA 
CTAATTCCTG GCAATGATCA GATCGACAAC ATGGACTCCA ATGTGAAGAA ATACGACTCT 
ACAGGGATGT TTCACTGGTG CGCAGCCAAG GAGATCGAGA AAGTCATCCT GACTCGAAGC 
GAAGCTGCCA TGACTGTCCT GAGTGGCCAC GTCGTAGTCT GCATCTTTGG GGATGTCAGC 
TCAGCCCTGA TTGGCCTCCG GAACCTGGTG ATGCCGCTTC GTGCCAGCAA CTTCCACTAC 
CATGAGCTCA AACACATTGT GTTTGTGGGA TCCATTGAGT ACCTCAAGCG GGAATGGGAG 
ACACTTCACA ACTTCCCCAA AGTGTCCATA TTGCCGGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATCGGGGTC TTGCAGGCTA ATTCCCAAGG ATTCACACCT 
CCAGGAATGG ACAGATCCTC TCCTGACAAC AGCCCAGTGC ACGGGATGTT ACGGCAGCCA 
TCCATCACGA CTGGGGTCAA TATCCCCATC ATCACAGAAC TCGTGAATGA CACTAATGTT 
CAGTTTTTGG ACCAAGATGA TGACGATGAC CCCGACACAG AGCTGTACCT CACGCAACCC 
TTTGCGTGTG GGACAGCTTT TGCCGTCAGC GTCCTGGACT CGCTCATGAG TGCGACATAC 
TTCAATGACA ATATCCTCAC CCTGATACGG ACCTTGGTGA CAGGAGGAGC CACACCAGAG 
CTCGAGGCTC TGATTGCTGA GGAGAATGCA CTTCGAGGGG GCTACAGCAC TCCCCAGACA 
CTGGCCAACA GGGACCGTTG CAGAGTGGCC CAGTTAGCTC TGTTGGATGG GCCCTTTGCA 
GACTTAGGGG ATGGTGGTTG TTATGGTGAT TTGTTCTGCA AAGCTCTGAA AACATATAAT 
ATGCTTTGTT TTGGAATTTA CCGGCTGAGA GATGCCCACC TCAGCACCCC CAGCCAGTGT 
ACGAAAAGGT ACGTCATCAC CAACCCCCCC TACGAGTTTG AGCTGGTGCC GACAGACCTG 
ATCTTCTGCC TGATGCAGTT TGACCACAAC GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT 
TCCTCCCACT CATCACAGTC CTCCAGCAAG AAGAGCTCCT CTGTCCACTC CATCCCGTCC 
ACAGCAAACC GGCCAAACCG GCCCAAGTCC AGGGAGTCCC GCGACAAACA GAAAAAAGAA 
ATGGTTTACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849871.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848093.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ONa32289
Clone ID Related Accession (Same CDS sequence)	XM_008849871.2
Accession Version	XM_008849871.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGTGAATG ACACTAATGT TCAGTTTTTG 
GACCAAGATG ATGACGATGA CCCCGACACA GAGCTGTACC TCACGCAACC CTTTGCGTGT 
GGGACAGCTT TTGCCGTCAG CGTCCTGGAC TCGCTCATGA GTGCGACATA CTTCAATGAC 
AATATCCTCA CCCTGATACG GACCTTGGTG ACAGGAGGAG CCACACCAGA GCTCGAGGCT 
CTGATTGCTG AGGAGAATGC ACTTCGAGGG GGCTACAGCA CTCCCCAGAC ACTGGCCAAC 
AGGGACCGTT GCAGAGTGGC CCAGTTAGCT CTGTTGGATG GGCCCTTTGC AGACTTAGGG 
GATGGTGGTT GTTATGGTGA TTTGTTCTGC AAAGCTCTGA AAACATATAA TATGCTTTGT 
TTTGGAATTT ACCGGCTGAG AGATGCCCAC CTCAGCACCC CCAGCCAGTG TACGAAAAGG 
TACGTCATCA CCAACCCCCC CTACGAGTTT GAGCTGGTGC CGACAGACCT GATCTTCTGC 
CTGATGCAGT TTGACCACAA CGCCGGCCAG TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC 
TCATCACAGT CCTCCAGCAA GAAGAGCTCC TCTGTCCACT CCATCCCGTC CACAGCAAAC 
CGGCCAAACC GGCCCAAGTC CAGGGAGTCC CGCGACAAAC AGAACAGAAA AGAAATGGTT 
TACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848093.1
CDS	156..3884
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_008849871.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849871.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGTGAATG ACACTAATGT TCAGTTTTTG 
GACCAAGATG ATGACGATGA CCCCGACACA GAGCTGTACC TCACGCAACC CTTTGCGTGT 
GGGACAGCTT TTGCCGTCAG CGTCCTGGAC TCGCTCATGA GTGCGACATA CTTCAATGAC 
AATATCCTCA CCCTGATACG GACCTTGGTG ACAGGAGGAG CCACACCAGA GCTCGAGGCT 
CTGATTGCTG AGGAGAATGC ACTTCGAGGG GGCTACAGCA CTCCCCAGAC ACTGGCCAAC 
AGGGACCGTT GCAGAGTGGC CCAGTTAGCT CTGTTGGATG GGCCCTTTGC AGACTTAGGG 
GATGGTGGTT GTTATGGTGA TTTGTTCTGC AAAGCTCTGA AAACATATAA TATGCTTTGT 
TTTGGAATTT ACCGGCTGAG AGATGCCCAC CTCAGCACCC CCAGCCAGTG TACGAAAAGG 
TACGTCATCA CCAACCCCCC CTACGAGTTT GAGCTGGTGC CGACAGACCT GATCTTCTGC 
CTGATGCAGT TTGACCACAA CGCCGGCCAG TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC 
TCATCACAGT CCTCCAGCAA GAAGAGCTCC TCTGTCCACT CCATCCCGTC CACAGCAAAC 
CGGCCAAACC GGCCCAAGTC CAGGGAGTCC CGCGACAAAC AGAACAGAAA AGAAATGGTT 
TACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849872.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848094.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ONa32290
Clone ID Related Accession (Same CDS sequence)	XM_008849872.2
Accession Version	XM_008849872.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGTGAATG ACACTAATGT TCAGTTTTTG 
GACCAAGATG ATGACGATGA CCCCGACACA GAGCTGTACC TCACGCAACC CTTTGCGTGT 
GGGACAGCTT TTGCCGTCAG CGTCCTGGAC TCGCTCATGA GTGCGACATA CTTCAATGAC 
AATATCCTCA CCCTGATACG GACCTTGGTG ACAGGAGGAG CCACACCAGA GCTCGAGGCT 
CTGATTGCTG AGGAGAATGC ACTTCGAGGG GGCTACAGCA CTCCCCAGAC ACTGGCCAAC 
AGGGACCGTT GCAGAGTGGC CCAGTTAGCT CTGTTGGATG GGCCCTTTGC AGACTTAGGG 
GATGGTGGTT GTTATGGTGA TTTGTTCTGC AAAGCTCTGA AAACATATAA TATGCTTTGT 
TTTGGAATTT ACCGGCTGAG AGATGCCCAC CTCAGCACCC CCAGCCAGTG TACGAAAAGG 
TACGTCATCA CCAACCCCCC CTACGAGTTT GAGCTGGTGC CGACAGACCT GATCTTCTGC 
CTGATGCAGT TTGACCACAA CGCCGGCCAG TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC 
TCATCACAGT CCTCCAGCAA GAAGAGCTCC TCTGTCCACT CCATCCCGTC CACAGCAAAC 
CGGCCAAACC GGCCCAAGTC CAGGGAGTCC CGCGACAAAC AGAAAAAAGA AATGGTTTAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848094.1
CDS	156..3881
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR

Target ORF information:

RefSeq Version	XM_008849872.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849872.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CCAAGACGTC CATCTACAAG AGAATGAGCC GAGCATGTTG TTTTGATTGC 
GGACGTTCTG AGCGTGACTG CTCATGCATG TCAGGCCGTG TGCGTGGTAA CGTGGACACC 
CCTGAGAGAA CCTTCCCGCT TTCTTCTGTC TCTGTTAATG ATTGCGCCAC CAGTTTCCGT 
GCCTTTGAAG ATGAACAGCC GTCAACGCTG TCACCCAAAA AAAAACAGCG CAACGGGGGC 
ATGAGGAACT CGCCCAACTC CTCCCCGAAG CTGATGAGGC ATGACCCCTT ACTAATTCCT 
GGCAATGATC AGATCGACAA CATGGACTCC AATGTGAAGA AATACGACTC TACAGGGATG 
TTTCACTGGT GCGCAGCCAA GGAGATCGAG AAAGTCATCC TGACTCGAAG CGAAGCTGCC 
ATGACTGTCC TGAGTGGCCA CGTCGTAGTC TGCATCTTTG GGGATGTCAG CTCAGCCCTG 
ATTGGCCTCC GGAACCTGGT GATGCCGCTT CGTGCCAGCA ACTTCCACTA CCATGAGCTC 
AAACACATTG TGTTTGTGGG ATCCATTGAG TACCTCAAGC GGGAATGGGA GACACTTCAC 
AACTTCCCCA AAGTGTCCAT ATTGCCGGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGTGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATACTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATCGGGGT CTTGCAGGCT AATTCCCAAG GATTCACACC TCCAGGAATG 
GACAGATCCT CTCCTGACAA CAGCCCAGTG CACGGGATGT TACGGCAGCC ATCCATCACG 
ACTGGGGTCA ATATCCCCAT CATCACAGAA CTCGTGAATG ACACTAATGT TCAGTTTTTG 
GACCAAGATG ATGACGATGA CCCCGACACA GAGCTGTACC TCACGCAACC CTTTGCGTGT 
GGGACAGCTT TTGCCGTCAG CGTCCTGGAC TCGCTCATGA GTGCGACATA CTTCAATGAC 
AATATCCTCA CCCTGATACG GACCTTGGTG ACAGGAGGAG CCACACCAGA GCTCGAGGCT 
CTGATTGCTG AGGAGAATGC ACTTCGAGGG GGCTACAGCA CTCCCCAGAC ACTGGCCAAC 
AGGGACCGTT GCAGAGTGGC CCAGTTAGCT CTGTTGGATG GGCCCTTTGC AGACTTAGGG 
GATGGTGGTT GTTATGGTGA TTTGTTCTGC AAAGCTCTGA AAACATATAA TATGCTTTGT 
TTTGGAATTT ACCGGCTGAG AGATGCCCAC CTCAGCACCC CCAGCCAGTG TACGAAAAGG 
TACGTCATCA CCAACCCCCC CTACGAGTTT GAGCTGGTGC CGACAGACCT GATCTTCTGC 
CTGATGCAGT TTGACCACAA CGCCGGCCAG TCCCGGGCCA GCCTGTCCCA TTCCTCCCAC 
TCATCACAGT CCTCCAGCAA GAAGAGCTCC TCTGTCCACT CCATCCCGTC CACAGCAAAC 
CGGCCAAACC GGCCCAAGTC CAGGGAGTCC CGCGACAAAC AGAAAAAAGA AATGGTTTAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849873.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848095.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X13, mRNA.

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CloneID	ONa32291
Clone ID Related Accession (Same CDS sequence)	XM_008849873.2
Accession Version	XM_008849873.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCCC 
AAGACGTCCA TCTACAAGAG AATGAGCCGA GCATGTTGTT TTGATTGCGG ACGTTCTGAG 
CGTGACTGCT CATGCATGTC AGGCCGTGTG CGTGGTAACG TGGACACCCC TGAGAGAACC 
TTCCCGCTTT CTTCTGTCTC TGTTAATGAT TGCGCCACCA GTTTCCGTGC CTTTGAAGAT 
GAACAGCCGT CAACGCTGTC ACCCAAAAAA AAACAGCGCA ACGGGGGCAT GAGGAACTCG 
CCCAACTCCT CCCCGAAGCT GATGAGGCAT GACCCCTTAC TAATTCCTGG CAATGATCAG 
ATCGACAACA TGGACTCCAA TGTGAAGAAA TACGACTCTA CAGGGATGTT TCACTGGTGC 
GCAGCCAAGG AGATCGAGAA AGTCATCCTG ACTCGAAGCG AAGCTGCCAT GACTGTCCTG 
AGTGGCCACG TCGTAGTCTG CATCTTTGGG GATGTCAGCT CAGCCCTGAT TGGCCTCCGG 
AACCTGGTGA TGCCGCTTCG TGCCAGCAAC TTCCACTACC ATGAGCTCAA ACACATTGTG 
TTTGTGGGAT CCATTGAGTA CCTCAAGCGG GAATGGGAGA CACTTCACAA CTTCCCCAAA 
GTGTCCATAT TGCCGGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGTGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TACTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATCGGGGTCT TGCAGGCTAA TTCCCAAGGA TTCACACCTC CAGGAATGGA CAGATCCTCT 
CCTGACAACA GCCCAGTGCA CGGGATGTTA CGGCAGCCAT CCATCACGAC TGGGGTCAAT 
ATCCCCATCA TCACAGAACT CGTGAATGAC ACTAATGTTC AGTTTTTGGA CCAAGATGAT 
GACGATGACC CCGACACAGA GCTGTACCTC ACGCAACCCT TTGCGTGTGG GACAGCTTTT 
GCCGTCAGCG TCCTGGACTC GCTCATGAGT GCGACATACT TCAATGACAA TATCCTCACC 
CTGATACGGA CCTTGGTGAC AGGAGGAGCC ACACCAGAGC TCGAGGCTCT GATTGCTGAG 
GAGAATGCAC TTCGAGGGGG CTACAGCACT CCCCAGACAC TGGCCAACAG GGACCGTTGC 
AGAGTGGCCC AGTTAGCTCT GTTGGATGGG CCCTTTGCAG ACTTAGGGGA TGGTGGTTGT 
TATGGTGATT TGTTCTGCAA AGCTCTGAAA ACATATAATA TGCTTTGTTT TGGAATTTAC 
CGGCTGAGAG ATGCCCACCT CAGCACCCCC AGCCAGTGTA CGAAAAGGTA CGTCATCACC 
AACCCCCCCT ACGAGTTTGA GCTGGTGCCG ACAGACCTGA TCTTCTGCCT GATGCAGTTT 
GACCACAACG CCGGCCAGTC CCGGGCCAGC CTGTCCCATT CCTCCCACTC ATCACAGTCC 
TCCAGCAAGA AGAGCTCCTC TGTCCACTCC ATCCCGTCCA CAGCAAACCG GCCAAACCGG 
CCCAAGTCCA GGGAGTCCCG CGACAAACAG AACAGAAAAG AAATGGTTTA CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848095.1
CDS	156..3872
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_008849873.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849873.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCCC 
AAGACGTCCA TCTACAAGAG AATGAGCCGA GCATGTTGTT TTGATTGCGG ACGTTCTGAG 
CGTGACTGCT CATGCATGTC AGGCCGTGTG CGTGGTAACG TGGACACCCC TGAGAGAACC 
TTCCCGCTTT CTTCTGTCTC TGTTAATGAT TGCGCCACCA GTTTCCGTGC CTTTGAAGAT 
GAACAGCCGT CAACGCTGTC ACCCAAAAAA AAACAGCGCA ACGGGGGCAT GAGGAACTCG 
CCCAACTCCT CCCCGAAGCT GATGAGGCAT GACCCCTTAC TAATTCCTGG CAATGATCAG 
ATCGACAACA TGGACTCCAA TGTGAAGAAA TACGACTCTA CAGGGATGTT TCACTGGTGC 
GCAGCCAAGG AGATCGAGAA AGTCATCCTG ACTCGAAGCG AAGCTGCCAT GACTGTCCTG 
AGTGGCCACG TCGTAGTCTG CATCTTTGGG GATGTCAGCT CAGCCCTGAT TGGCCTCCGG 
AACCTGGTGA TGCCGCTTCG TGCCAGCAAC TTCCACTACC ATGAGCTCAA ACACATTGTG 
TTTGTGGGAT CCATTGAGTA CCTCAAGCGG GAATGGGAGA CACTTCACAA CTTCCCCAAA 
GTGTCCATAT TGCCGGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGTGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TACTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATCGGGGTCT TGCAGGCTAA TTCCCAAGGA TTCACACCTC CAGGAATGGA CAGATCCTCT 
CCTGACAACA GCCCAGTGCA CGGGATGTTA CGGCAGCCAT CCATCACGAC TGGGGTCAAT 
ATCCCCATCA TCACAGAACT CGTGAATGAC ACTAATGTTC AGTTTTTGGA CCAAGATGAT 
GACGATGACC CCGACACAGA GCTGTACCTC ACGCAACCCT TTGCGTGTGG GACAGCTTTT 
GCCGTCAGCG TCCTGGACTC GCTCATGAGT GCGACATACT TCAATGACAA TATCCTCACC 
CTGATACGGA CCTTGGTGAC AGGAGGAGCC ACACCAGAGC TCGAGGCTCT GATTGCTGAG 
GAGAATGCAC TTCGAGGGGG CTACAGCACT CCCCAGACAC TGGCCAACAG GGACCGTTGC 
AGAGTGGCCC AGTTAGCTCT GTTGGATGGG CCCTTTGCAG ACTTAGGGGA TGGTGGTTGT 
TATGGTGATT TGTTCTGCAA AGCTCTGAAA ACATATAATA TGCTTTGTTT TGGAATTTAC 
CGGCTGAGAG ATGCCCACCT CAGCACCCCC AGCCAGTGTA CGAAAAGGTA CGTCATCACC 
AACCCCCCCT ACGAGTTTGA GCTGGTGCCG ACAGACCTGA TCTTCTGCCT GATGCAGTTT 
GACCACAACG CCGGCCAGTC CCGGGCCAGC CTGTCCCATT CCTCCCACTC ATCACAGTCC 
TCCAGCAAGA AGAGCTCCTC TGTCCACTCC ATCCCGTCCA CAGCAAACCG GCCAAACCGG 
CCCAAGTCCA GGGAGTCCCG CGACAAACAG AACAGAAAAG AAATGGTTTA CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849875.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848097.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ONa32292
Clone ID Related Accession (Same CDS sequence)	XM_008849875.2
Accession Version	XM_008849875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTT 
GAAGATGAAC AGCCGTCAAC GCTGTCACCC AAAAAAAAAC AGCGCAACGG GGGCATGAGG 
AACTCGCCCA ACTCCTCCCC GAAGCTGATG AGGCATGACC CCTTACTAAT TCCTGGCAAT 
GATCAGATCG ACAACATGGA CTCCAATGTG AAGAAATACG ACTCTACAGG GATGTTTCAC 
TGGTGCGCAG CCAAGGAGAT CGAGAAAGTC ATCCTGACTC GAAGCGAAGC TGCCATGACT 
GTCCTGAGTG GCCACGTCGT AGTCTGCATC TTTGGGGATG TCAGCTCAGC CCTGATTGGC 
CTCCGGAACC TGGTGATGCC GCTTCGTGCC AGCAACTTCC ACTACCATGA GCTCAAACAC 
ATTGTGTTTG TGGGATCCAT TGAGTACCTC AAGCGGGAAT GGGAGACACT TCACAACTTC 
CCCAAAGTGT CCATATTGCC GGGTACGCCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATACT 
TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA CACCTCCAGG AATGGACAGA 
TCCTCTCCTG ACAACAGCCC AGTGCACGGG ATGTTACGGC AGCCATCCAT CACGACTGGG 
GTCAATATCC CCATCATCAC AGAACTCGTG AATGACACTA ATGTTCAGTT TTTGGACCAA 
GATGATGACG ATGACCCCGA CACAGAGCTG TACCTCACGC AACCCTTTGC GTGTGGGACA 
GCTTTTGCCG TCAGCGTCCT GGACTCGCTC ATGAGTGCGA CATACTTCAA TGACAATATC 
CTCACCCTGA TACGGACCTT GGTGACAGGA GGAGCCACAC CAGAGCTCGA GGCTCTGATT 
GCTGAGGAGA ATGCACTTCG AGGGGGCTAC AGCACTCCCC AGACACTGGC CAACAGGGAC 
CGTTGCAGAG TGGCCCAGTT AGCTCTGTTG GATGGGCCCT TTGCAGACTT AGGGGATGGT 
GGTTGTTATG GTGATTTGTT CTGCAAAGCT CTGAAAACAT ATAATATGCT TTGTTTTGGA 
ATTTACCGGC TGAGAGATGC CCACCTCAGC ACCCCCAGCC AGTGTACGAA AAGGTACGTC 
ATCACCAACC CCCCCTACGA GTTTGAGCTG GTGCCGACAG ACCTGATCTT CTGCCTGATG 
CAGTTTGACC ACAACGCCGG CCAGTCCCGG GCCAGCCTGT CCCATTCCTC CCACTCATCA 
CAGTCCTCCA GCAAGAAGAG CTCCTCTGTC CACTCCATCC CGTCCACAGC AAACCGGCCA 
AACCGGCCCA AGTCCAGGGA GTCCCGCGAC AAACAGAATG CAACAAGGAT GAATAGGATG 
GGCCAAGCAG AAAAGAAATG GTTTACAGAT GAACCGGATA ATGCCTATCC CAGAAACATT 
CAAATCAAGC CCATGAGTAC CCACATGGCT AACCAGATCA ACCAATATAA ATCCACAAGC 
AGCCTGATCC CTCCAATCAG AGAAGTTGAA GATGAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848097.1
CDS	156..3857
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_008849875.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849875.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTT 
GAAGATGAAC AGCCGTCAAC GCTGTCACCC AAAAAAAAAC AGCGCAACGG GGGCATGAGG 
AACTCGCCCA ACTCCTCCCC GAAGCTGATG AGGCATGACC CCTTACTAAT TCCTGGCAAT 
GATCAGATCG ACAACATGGA CTCCAATGTG AAGAAATACG ACTCTACAGG GATGTTTCAC 
TGGTGCGCAG CCAAGGAGAT CGAGAAAGTC ATCCTGACTC GAAGCGAAGC TGCCATGACT 
GTCCTGAGTG GCCACGTCGT AGTCTGCATC TTTGGGGATG TCAGCTCAGC CCTGATTGGC 
CTCCGGAACC TGGTGATGCC GCTTCGTGCC AGCAACTTCC ACTACCATGA GCTCAAACAC 
ATTGTGTTTG TGGGATCCAT TGAGTACCTC AAGCGGGAAT GGGAGACACT TCACAACTTC 
CCCAAAGTGT CCATATTGCC GGGTACGCCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATACT 
TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA CACCTCCAGG AATGGACAGA 
TCCTCTCCTG ACAACAGCCC AGTGCACGGG ATGTTACGGC AGCCATCCAT CACGACTGGG 
GTCAATATCC CCATCATCAC AGAACTCGTG AATGACACTA ATGTTCAGTT TTTGGACCAA 
GATGATGACG ATGACCCCGA CACAGAGCTG TACCTCACGC AACCCTTTGC GTGTGGGACA 
GCTTTTGCCG TCAGCGTCCT GGACTCGCTC ATGAGTGCGA CATACTTCAA TGACAATATC 
CTCACCCTGA TACGGACCTT GGTGACAGGA GGAGCCACAC CAGAGCTCGA GGCTCTGATT 
GCTGAGGAGA ATGCACTTCG AGGGGGCTAC AGCACTCCCC AGACACTGGC CAACAGGGAC 
CGTTGCAGAG TGGCCCAGTT AGCTCTGTTG GATGGGCCCT TTGCAGACTT AGGGGATGGT 
GGTTGTTATG GTGATTTGTT CTGCAAAGCT CTGAAAACAT ATAATATGCT TTGTTTTGGA 
ATTTACCGGC TGAGAGATGC CCACCTCAGC ACCCCCAGCC AGTGTACGAA AAGGTACGTC 
ATCACCAACC CCCCCTACGA GTTTGAGCTG GTGCCGACAG ACCTGATCTT CTGCCTGATG 
CAGTTTGACC ACAACGCCGG CCAGTCCCGG GCCAGCCTGT CCCATTCCTC CCACTCATCA 
CAGTCCTCCA GCAAGAAGAG CTCCTCTGTC CACTCCATCC CGTCCACAGC AAACCGGCCA 
AACCGGCCCA AGTCCAGGGA GTCCCGCGAC AAACAGAATG CAACAAGGAT GAATAGGATG 
GGCCAAGCAG AAAAGAAATG GTTTACAGAT GAACCGGATA ATGCCTATCC CAGAAACATT 
CAAATCAAGC CCATGAGTAC CCACATGGCT AACCAGATCA ACCAATATAA ATCCACAAGC 
AGCCTGATCC CTCCAATCAG AGAAGTTGAA GATGAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849876.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848098.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	ONa32293
Clone ID Related Accession (Same CDS sequence)	XM_008849876.2
Accession Version	XM_008849876.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849876.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTT 
GAAGATGAAC AGCCGTCAAC GCTGTCACCC AAAAAAAAAC AGCGCAACGG GGGCATGAGG 
AACTCGCCCA ACTCCTCCCC GAAGCTGATG AGGCATGACC CCTTACTAAT TCCTGGCAAT 
GATCAGATCG ACAACATGGA CTCCAATGTG AAGAAATACG ACTCTACAGG GATGTTTCAC 
TGGTGCGCAG CCAAGGAGAT CGAGAAAGTC ATCCTGACTC GAAGCGAAGC TGCCATGACT 
GTCCTGAGTG GCCACGTCGT AGTCTGCATC TTTGGGGATG TCAGCTCAGC CCTGATTGGC 
CTCCGGAACC TGGTGATGCC GCTTCGTGCC AGCAACTTCC ACTACCATGA GCTCAAACAC 
ATTGTGTTTG TGGGATCCAT TGAGTACCTC AAGCGGGAAT GGGAGACACT TCACAACTTC 
CCCAAAGTGT CCATATTGCC GGGTACGCCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATACT 
TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA CACCTCCAGG AATGGACAGA 
TCCTCTCCTG ACAACAGCCC AGTGCACGGG ATGTTACGGC AGCCATCCAT CACGACTGGG 
GTCAATATCC CCATCATCAC AGAACTCGTG AATGACACTA ATGTTCAGTT TTTGGACCAA 
GATGATGACG ATGACCCCGA CACAGAGCTG TACCTCACGC AACCCTTTGC GTGTGGGACA 
GCTTTTGCCG TCAGCGTCCT GGACTCGCTC ATGAGTGCGA CATACTTCAA TGACAATATC 
CTCACCCTGA TACGGACCTT GGTGACAGGA GGAGCCACAC CAGAGCTCGA GGCTCTGATT 
GCTGAGGAGA ATGCACTTCG AGGGGGCTAC AGCACTCCCC AGACACTGGC CAACAGGGAC 
CGTTGCAGAG TGGCCCAGTT AGCTCTGTTG GATGGGCCCT TTGCAGACTT AGGGGATGGT 
GGTTGTTATG GTGATTTGTT CTGCAAAGCT CTGAAAACAT ATAATATGCT TTGTTTTGGA 
ATTTACCGGC TGAGAGATGC CCACCTCAGC ACCCCCAGCC AGTGTACGAA AAGGTACGTC 
ATCACCAACC CCCCCTACGA GTTTGAGCTG GTGCCGACAG ACCTGATCTT CTGCCTGATG 
CAGTTTGACC ACAACGCCGG CCAGTCCCGG GCCAGCCTGT CCCATTCCTC CCACTCATCA 
CAGTCCTCCA GCAAGAAGAG CTCCTCTGTC CACTCCATCC CGTCCACAGC AAACCGGCCA 
AACCGGCCCA AGTCCAGGGA GTCCCGCGAC AAACAGAATG CAACAAGGAT GAATAGGATG 
GGCCAAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA 
ATCAAGCCCA TGAGTACCCA CATGGCTAAC CAGATCAACC AATATAAATC CACAAGCAGC 
CTGATCCCTC CAATCAGAGA AGTTGAAGAT GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848098.1
CDS	156..3854
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_008849876.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849876.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTT 
GAAGATGAAC AGCCGTCAAC GCTGTCACCC AAAAAAAAAC AGCGCAACGG GGGCATGAGG 
AACTCGCCCA ACTCCTCCCC GAAGCTGATG AGGCATGACC CCTTACTAAT TCCTGGCAAT 
GATCAGATCG ACAACATGGA CTCCAATGTG AAGAAATACG ACTCTACAGG GATGTTTCAC 
TGGTGCGCAG CCAAGGAGAT CGAGAAAGTC ATCCTGACTC GAAGCGAAGC TGCCATGACT 
GTCCTGAGTG GCCACGTCGT AGTCTGCATC TTTGGGGATG TCAGCTCAGC CCTGATTGGC 
CTCCGGAACC TGGTGATGCC GCTTCGTGCC AGCAACTTCC ACTACCATGA GCTCAAACAC 
ATTGTGTTTG TGGGATCCAT TGAGTACCTC AAGCGGGAAT GGGAGACACT TCACAACTTC 
CCCAAAGTGT CCATATTGCC GGGTACGCCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATACT 
TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA CACCTCCAGG AATGGACAGA 
TCCTCTCCTG ACAACAGCCC AGTGCACGGG ATGTTACGGC AGCCATCCAT CACGACTGGG 
GTCAATATCC CCATCATCAC AGAACTCGTG AATGACACTA ATGTTCAGTT TTTGGACCAA 
GATGATGACG ATGACCCCGA CACAGAGCTG TACCTCACGC AACCCTTTGC GTGTGGGACA 
GCTTTTGCCG TCAGCGTCCT GGACTCGCTC ATGAGTGCGA CATACTTCAA TGACAATATC 
CTCACCCTGA TACGGACCTT GGTGACAGGA GGAGCCACAC CAGAGCTCGA GGCTCTGATT 
GCTGAGGAGA ATGCACTTCG AGGGGGCTAC AGCACTCCCC AGACACTGGC CAACAGGGAC 
CGTTGCAGAG TGGCCCAGTT AGCTCTGTTG GATGGGCCCT TTGCAGACTT AGGGGATGGT 
GGTTGTTATG GTGATTTGTT CTGCAAAGCT CTGAAAACAT ATAATATGCT TTGTTTTGGA 
ATTTACCGGC TGAGAGATGC CCACCTCAGC ACCCCCAGCC AGTGTACGAA AAGGTACGTC 
ATCACCAACC CCCCCTACGA GTTTGAGCTG GTGCCGACAG ACCTGATCTT CTGCCTGATG 
CAGTTTGACC ACAACGCCGG CCAGTCCCGG GCCAGCCTGT CCCATTCCTC CCACTCATCA 
CAGTCCTCCA GCAAGAAGAG CTCCTCTGTC CACTCCATCC CGTCCACAGC AAACCGGCCA 
AACCGGCCCA AGTCCAGGGA GTCCCGCGAC AAACAGAATG CAACAAGGAT GAATAGGATG 
GGCCAAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA 
ATCAAGCCCA TGAGTACCCA CATGGCTAAC CAGATCAACC AATATAAATC CACAAGCAGC 
CTGATCCCTC CAATCAGAGA AGTTGAAGAT GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849878.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848100.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	ONa32294
Clone ID Related Accession (Same CDS sequence)	XM_008849878.1
Accession Version	XM_008849878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CAGTCAAGGC TGCAGCTAGA TCATGCTATC ACAAAGACCC ATTTGTGCAC 
AAGAACGCAA CTCCCAAATC TCGGCTTGTG ACTGAGCCAG TTGAAGATGA ACAGCCGTCA 
ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC GGGGGCATGA GGAACTCGCC CAACTCCTCC 
CCGAAGCTGA TGAGGCATGA CCCCTTACTA ATTCCTGGCA ATGATCAGAT CGACAACATG 
GACTCCAATG TGAAGAAATA CGACTCTACA GGGATGTTTC ACTGGTGCGC AGCCAAGGAG 
ATCGAGAAAG TCATCCTGAC TCGAAGCGAA GCTGCCATGA CTGTCCTGAG TGGCCACGTC 
GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA GCCCTGATTG GCCTCCGGAA CCTGGTGATG 
CCGCTTCGTG CCAGCAACTT CCACTACCAT GAGCTCAAAC ACATTGTGTT TGTGGGATCC 
ATTGAGTACC TCAAGCGGGA ATGGGAGACA CTTCACAACT TCCCCAAAGT GTCCATATTG 
CCGGGTACGC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGTGACATG 
TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATA CTTCGCTGCA GGACAAGGAA 
TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT CGGGGTCTTG 
CAGGCTAATT CCCAAGGATT CACACCTCCA GGAATGGACA GATCCTCTCC TGACAACAGC 
CCAGTGCACG GGATGTTACG GCAGCCATCC ATCACGACTG GGGTCAATAT CCCCATCATC 
ACAGAACTCG TGAATGACAC TAATGTTCAG TTTTTGGACC AAGATGATGA CGATGACCCC 
GACACAGAGC TGTACCTCAC GCAACCCTTT GCGTGTGGGA CAGCTTTTGC CGTCAGCGTC 
CTGGACTCGC TCATGAGTGC GACATACTTC AATGACAATA TCCTCACCCT GATACGGACC 
TTGGTGACAG GAGGAGCCAC ACCAGAGCTC GAGGCTCTGA TTGCTGAGGA GAATGCACTT 
CGAGGGGGCT ACAGCACTCC CCAGACACTG GCCAACAGGG ACCGTTGCAG AGTGGCCCAG 
TTAGCTCTGT TGGATGGGCC CTTTGCAGAC TTAGGGGATG GTGGTTGTTA TGGTGATTTG 
TTCTGCAAAG CTCTGAAAAC ATATAATATG CTTTGTTTTG GAATTTACCG GCTGAGAGAT 
GCCCACCTCA GCACCCCCAG CCAGTGTACG AAAAGGTACG TCATCACCAA CCCCCCCTAC 
GAGTTTGAGC TGGTGCCGAC AGACCTGATC TTCTGCCTGA TGCAGTTTGA CCACAACGCC 
GGCCAGTCCC GGGCCAGCCT GTCCCATTCC TCCCACTCAT CACAGTCCTC CAGCAAGAAG 
AGCTCCTCTG TCCACTCCAT CCCGTCCACA GCAAACCGGC CAAACCGGCC CAAGTCCAGG 
GAGTCCCGCG ACAAACAGAA GTACGTTCAG GAAGAACGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848100.1
CDS	156..3800
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPVKAAARSCYHKDPFVHKNATPKSRLVTEPVEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKYVQEERL

Target ORF information:

RefSeq Version	XM_008849878.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849878.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CAGTCAAGGC TGCAGCTAGA TCATGCTATC ACAAAGACCC ATTTGTGCAC 
AAGAACGCAA CTCCCAAATC TCGGCTTGTG ACTGAGCCAG TTGAAGATGA ACAGCCGTCA 
ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC GGGGGCATGA GGAACTCGCC CAACTCCTCC 
CCGAAGCTGA TGAGGCATGA CCCCTTACTA ATTCCTGGCA ATGATCAGAT CGACAACATG 
GACTCCAATG TGAAGAAATA CGACTCTACA GGGATGTTTC ACTGGTGCGC AGCCAAGGAG 
ATCGAGAAAG TCATCCTGAC TCGAAGCGAA GCTGCCATGA CTGTCCTGAG TGGCCACGTC 
GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA GCCCTGATTG GCCTCCGGAA CCTGGTGATG 
CCGCTTCGTG CCAGCAACTT CCACTACCAT GAGCTCAAAC ACATTGTGTT TGTGGGATCC 
ATTGAGTACC TCAAGCGGGA ATGGGAGACA CTTCACAACT TCCCCAAAGT GTCCATATTG 
CCGGGTACGC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGTGACATG 
TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATA CTTCGCTGCA GGACAAGGAA 
TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT CGGGGTCTTG 
CAGGCTAATT CCCAAGGATT CACACCTCCA GGAATGGACA GATCCTCTCC TGACAACAGC 
CCAGTGCACG GGATGTTACG GCAGCCATCC ATCACGACTG GGGTCAATAT CCCCATCATC 
ACAGAACTCG TGAATGACAC TAATGTTCAG TTTTTGGACC AAGATGATGA CGATGACCCC 
GACACAGAGC TGTACCTCAC GCAACCCTTT GCGTGTGGGA CAGCTTTTGC CGTCAGCGTC 
CTGGACTCGC TCATGAGTGC GACATACTTC AATGACAATA TCCTCACCCT GATACGGACC 
TTGGTGACAG GAGGAGCCAC ACCAGAGCTC GAGGCTCTGA TTGCTGAGGA GAATGCACTT 
CGAGGGGGCT ACAGCACTCC CCAGACACTG GCCAACAGGG ACCGTTGCAG AGTGGCCCAG 
TTAGCTCTGT TGGATGGGCC CTTTGCAGAC TTAGGGGATG GTGGTTGTTA TGGTGATTTG 
TTCTGCAAAG CTCTGAAAAC ATATAATATG CTTTGTTTTG GAATTTACCG GCTGAGAGAT 
GCCCACCTCA GCACCCCCAG CCAGTGTACG AAAAGGTACG TCATCACCAA CCCCCCCTAC 
GAGTTTGAGC TGGTGCCGAC AGACCTGATC TTCTGCCTGA TGCAGTTTGA CCACAACGCC 
GGCCAGTCCC GGGCCAGCCT GTCCCATTCC TCCCACTCAT CACAGTCCTC CAGCAAGAAG 
AGCTCCTCTG TCCACTCCAT CCCGTCCACA GCAAACCGGC CAAACCGGCC CAAGTCCAGG 
GAGTCCCGCG ACAAACAGAA GTACGTTCAG GAAGAACGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849877.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848099.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	ONa32295
Clone ID Related Accession (Same CDS sequence)	XM_008849877.2
Accession Version	XM_008849877.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849877.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CAGTCAAGGC TGCAGCTAGA TCATGCTATC ACAAAGACCC ATTTGTGCAC 
AAGAACGCAA CTCCCAAATC TCGGCTTGTG ACTGAGCCAG TTGAAGATGA ACAGCCGTCA 
ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC GGGGGCATGA GGAACTCGCC CAACTCCTCC 
CCGAAGCTGA TGAGGCATGA CCCCTTACTA ATTCCTGGCA ATGATCAGAT CGACAACATG 
GACTCCAATG TGAAGAAATA CGACTCTACA GGGATGTTTC ACTGGTGCGC AGCCAAGGAG 
ATCGAGAAAG TCATCCTGAC TCGAAGCGAA GCTGCCATGA CTGTCCTGAG TGGCCACGTC 
GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA GCCCTGATTG GCCTCCGGAA CCTGGTGATG 
CCGCTTCGTG CCAGCAACTT CCACTACCAT GAGCTCAAAC ACATTGTGTT TGTGGGATCC 
ATTGAGTACC TCAAGCGGGA ATGGGAGACA CTTCACAACT TCCCCAAAGT GTCCATATTG 
CCGGGTACGC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGTGACATG 
TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATA CTTCGCTGCA GGACAAGGAA 
TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT CGGGGTCTTG 
CAGGCTAATT CCCAAGGATT CACACCTCCA GGAATGGACA GATCCTCTCC TGACAACAGC 
CCAGTGCACG GGATGTTACG GCAGCCATCC ATCACGACTG GGGTCAATAT CCCCATCATC 
ACAGAACTCG TGAATGACAC TAATGTTCAG TTTTTGGACC AAGATGATGA CGATGACCCC 
GACACAGAGC TGTACCTCAC GCAACCCTTT GCGTGTGGGA CAGCTTTTGC CGTCAGCGTC 
CTGGACTCGC TCATGAGTGC GACATACTTC AATGACAATA TCCTCACCCT GATACGGACC 
TTGGTGACAG GAGGAGCCAC ACCAGAGCTC GAGGCTCTGA TTGCTGAGGA GAATGCACTT 
CGAGGGGGCT ACAGCACTCC CCAGACACTG GCCAACAGGG ACCGTTGCAG AGTGGCCCAG 
TTAGCTCTGT TGGATGGGCC CTTTGCAGAC TTAGGGGATG GTGGTTGTTA TGGTGATTTG 
TTCTGCAAAG CTCTGAAAAC ATATAATATG CTTTGTTTTG GAATTTACCG GCTGAGAGAT 
GCCCACCTCA GCACCCCCAG CCAGTGTACG AAAAGGTACG TCATCACCAA CCCCCCCTAC 
GAGTTTGAGC TGGTGCCGAC AGACCTGATC TTCTGCCTGA TGCAGTTTGA CCACAACGCC 
GGCCAGTCCC GGGCCAGCCT GTCCCATTCC TCCCACTCAT CACAGTCCTC CAGCAAGAAG 
AGCTCCTCTG TCCACTCCAT CCCGTCCACA GCAAACCGGC CAAACCGGCC CAAGTCCAGG 
GAGTCCCGCG ACAAACAGAA CAGAAAAGAA ATGGTTTACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848099.1
CDS	156..3800
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPVKAAARSCYHKDPFVHKNATPKSRLVTEPVEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_008849877.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849877.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CAGTCAAGGC TGCAGCTAGA TCATGCTATC ACAAAGACCC ATTTGTGCAC 
AAGAACGCAA CTCCCAAATC TCGGCTTGTG ACTGAGCCAG TTGAAGATGA ACAGCCGTCA 
ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC GGGGGCATGA GGAACTCGCC CAACTCCTCC 
CCGAAGCTGA TGAGGCATGA CCCCTTACTA ATTCCTGGCA ATGATCAGAT CGACAACATG 
GACTCCAATG TGAAGAAATA CGACTCTACA GGGATGTTTC ACTGGTGCGC AGCCAAGGAG 
ATCGAGAAAG TCATCCTGAC TCGAAGCGAA GCTGCCATGA CTGTCCTGAG TGGCCACGTC 
GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA GCCCTGATTG GCCTCCGGAA CCTGGTGATG 
CCGCTTCGTG CCAGCAACTT CCACTACCAT GAGCTCAAAC ACATTGTGTT TGTGGGATCC 
ATTGAGTACC TCAAGCGGGA ATGGGAGACA CTTCACAACT TCCCCAAAGT GTCCATATTG 
CCGGGTACGC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGTGACATG 
TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATA CTTCGCTGCA GGACAAGGAA 
TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT CGGGGTCTTG 
CAGGCTAATT CCCAAGGATT CACACCTCCA GGAATGGACA GATCCTCTCC TGACAACAGC 
CCAGTGCACG GGATGTTACG GCAGCCATCC ATCACGACTG GGGTCAATAT CCCCATCATC 
ACAGAACTCG TGAATGACAC TAATGTTCAG TTTTTGGACC AAGATGATGA CGATGACCCC 
GACACAGAGC TGTACCTCAC GCAACCCTTT GCGTGTGGGA CAGCTTTTGC CGTCAGCGTC 
CTGGACTCGC TCATGAGTGC GACATACTTC AATGACAATA TCCTCACCCT GATACGGACC 
TTGGTGACAG GAGGAGCCAC ACCAGAGCTC GAGGCTCTGA TTGCTGAGGA GAATGCACTT 
CGAGGGGGCT ACAGCACTCC CCAGACACTG GCCAACAGGG ACCGTTGCAG AGTGGCCCAG 
TTAGCTCTGT TGGATGGGCC CTTTGCAGAC TTAGGGGATG GTGGTTGTTA TGGTGATTTG 
TTCTGCAAAG CTCTGAAAAC ATATAATATG CTTTGTTTTG GAATTTACCG GCTGAGAGAT 
GCCCACCTCA GCACCCCCAG CCAGTGTACG AAAAGGTACG TCATCACCAA CCCCCCCTAC 
GAGTTTGAGC TGGTGCCGAC AGACCTGATC TTCTGCCTGA TGCAGTTTGA CCACAACGCC 
GGCCAGTCCC GGGCCAGCCT GTCCCATTCC TCCCACTCAT CACAGTCCTC CAGCAAGAAG 
AGCTCCTCTG TCCACTCCAT CCCGTCCACA GCAAACCGGC CAAACCGGCC CAAGTCCAGG 
GAGTCCCGCG ACAAACAGAA CAGAAAAGAA ATGGTTTACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_017802382.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017657871.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X23, mRNA.

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CloneID	ONa32296
Clone ID Related Accession (Same CDS sequence)	XM_017802382.1
Accession Version	XM_017802382.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CAGTCAAGGC TGCAGCTAGA TCATGCTATC ACAAAGACCC ATTTGTGCAC 
AAGAACGCAA CTCCCAAATC TCGGCTTGTG ACTGAGCCAG TTGAAGATGA ACAGCCGTCA 
ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC GGGGGCATGA GGAACTCGCC CAACTCCTCC 
CCGAAGCTGA TGAGGCATGA CCCCTTACTA ATTCCTGGCA ATGATCAGAT CGACAACATG 
GACTCCAATG TGAAGAAATA CGACTCTACA GGGATGTTTC ACTGGTGCGC AGCCAAGGAG 
ATCGAGAAAG TCATCCTGAC TCGAAGCGAA GCTGCCATGA CTGTCCTGAG TGGCCACGTC 
GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA GCCCTGATTG GCCTCCGGAA CCTGGTGATG 
CCGCTTCGTG CCAGCAACTT CCACTACCAT GAGCTCAAAC ACATTGTGTT TGTGGGATCC 
ATTGAGTACC TCAAGCGGGA ATGGGAGACA CTTCACAACT TCCCCAAAGT GTCCATATTG 
CCGGGTACGC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGTGACATG 
TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATA CTTCGCTGCA GGACAAGGAA 
TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT CGGGGTCTTG 
CAGGCTAATT CCCAAGGATT CACACCTCCA GGAATGGACA GATCCTCTCC TGACAACAGC 
CCAGTGCACG GGATGTTACG GCAGCCATCC ATCACGACTG GGGTCAATAT CCCCATCATC 
ACAGAACTCG TGAATGACAC TAATGTTCAG TTTTTGGACC AAGATGATGA CGATGACCCC 
GACACAGAGC TGTACCTCAC GCAACCCTTT GCGTGTGGGA CAGCTTTTGC CGTCAGCGTC 
CTGGACTCGC TCATGAGTGC GACATACTTC AATGACAATA TCCTCACCCT GATACGGACC 
TTGGTGACAG GAGGAGCCAC ACCAGAGCTC GAGGCTCTGA TTGCTGAGGA GAATGCACTT 
CGAGGGGGCT ACAGCACTCC CCAGACACTG GCCAACAGGG ACCGTTGCAG AGTGGCCCAG 
TTAGCTCTGT TGGATGGGCC CTTTGCAGAC TTAGGGGATG GTGGTTGTTA TGGTGATTTG 
TTCTGCAAAG CTCTGAAAAC ATATAATATG CTTTGTTTTG GAATTTACCG GCTGAGAGAT 
GCCCACCTCA GCACCCCCAG CCAGTGTACG AAAAGGTACG TCATCACCAA CCCCCCCTAC 
GAGTTTGAGC TGGTGCCGAC AGACCTGATC TTCTGCCTGA TGCAGTTTGA CCACAACGCC 
GGCCAGTCCC GGGCCAGCCT GTCCCATTCC TCCCACTCAT CACAGTCCTC CAGCAAGAAG 
AGCTCCTCTG TCCACTCCAT CCCGTCCACA GCAAACCGGC CAAACCGGCC CAAGTCCAGG 
GAGTCCCGCG ACAAACAGAA AAAAGAAATG GTTTACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017657871.1
CDS	156..3797
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRPVKAAARSCYHKDPFVHKNATPKSRLVTEPVEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR

Target ORF information:

RefSeq Version	XM_017802382.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017802382.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC CAGTCAAGGC TGCAGCTAGA TCATGCTATC ACAAAGACCC ATTTGTGCAC 
AAGAACGCAA CTCCCAAATC TCGGCTTGTG ACTGAGCCAG TTGAAGATGA ACAGCCGTCA 
ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC GGGGGCATGA GGAACTCGCC CAACTCCTCC 
CCGAAGCTGA TGAGGCATGA CCCCTTACTA ATTCCTGGCA ATGATCAGAT CGACAACATG 
GACTCCAATG TGAAGAAATA CGACTCTACA GGGATGTTTC ACTGGTGCGC AGCCAAGGAG 
ATCGAGAAAG TCATCCTGAC TCGAAGCGAA GCTGCCATGA CTGTCCTGAG TGGCCACGTC 
GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA GCCCTGATTG GCCTCCGGAA CCTGGTGATG 
CCGCTTCGTG CCAGCAACTT CCACTACCAT GAGCTCAAAC ACATTGTGTT TGTGGGATCC 
ATTGAGTACC TCAAGCGGGA ATGGGAGACA CTTCACAACT TCCCCAAAGT GTCCATATTG 
CCGGGTACGC CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGTGACATG 
TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATA CTTCGCTGCA GGACAAGGAA 
TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT CGGGGTCTTG 
CAGGCTAATT CCCAAGGATT CACACCTCCA GGAATGGACA GATCCTCTCC TGACAACAGC 
CCAGTGCACG GGATGTTACG GCAGCCATCC ATCACGACTG GGGTCAATAT CCCCATCATC 
ACAGAACTCG TGAATGACAC TAATGTTCAG TTTTTGGACC AAGATGATGA CGATGACCCC 
GACACAGAGC TGTACCTCAC GCAACCCTTT GCGTGTGGGA CAGCTTTTGC CGTCAGCGTC 
CTGGACTCGC TCATGAGTGC GACATACTTC AATGACAATA TCCTCACCCT GATACGGACC 
TTGGTGACAG GAGGAGCCAC ACCAGAGCTC GAGGCTCTGA TTGCTGAGGA GAATGCACTT 
CGAGGGGGCT ACAGCACTCC CCAGACACTG GCCAACAGGG ACCGTTGCAG AGTGGCCCAG 
TTAGCTCTGT TGGATGGGCC CTTTGCAGAC TTAGGGGATG GTGGTTGTTA TGGTGATTTG 
TTCTGCAAAG CTCTGAAAAC ATATAATATG CTTTGTTTTG GAATTTACCG GCTGAGAGAT 
GCCCACCTCA GCACCCCCAG CCAGTGTACG AAAAGGTACG TCATCACCAA CCCCCCCTAC 
GAGTTTGAGC TGGTGCCGAC AGACCTGATC TTCTGCCTGA TGCAGTTTGA CCACAACGCC 
GGCCAGTCCC GGGCCAGCCT GTCCCATTCC TCCCACTCAT CACAGTCCTC CAGCAAGAAG 
AGCTCCTCTG TCCACTCCAT CCCGTCCACA GCAAACCGGC CAAACCGGCC CAAGTCCAGG 
GAGTCCCGCG ACAAACAGAA AAAAGAAATG GTTTACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849880.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848102.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ONa32297
Clone ID Related Accession (Same CDS sequence)	XM_008849880.2
Accession Version	XM_008849880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TTGAAGATGA ACAGCCGTCA ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC 
GGGGGCATGA GGAACTCGCC CAACTCCTCC CCGAAGCTGA TGAGGCATGA CCCCTTACTA 
ATTCCTGGCA ATGATCAGAT CGACAACATG GACTCCAATG TGAAGAAATA CGACTCTACA 
GGGATGTTTC ACTGGTGCGC AGCCAAGGAG ATCGAGAAAG TCATCCTGAC TCGAAGCGAA 
GCTGCCATGA CTGTCCTGAG TGGCCACGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA 
GCCCTGATTG GCCTCCGGAA CCTGGTGATG CCGCTTCGTG CCAGCAACTT CCACTACCAT 
GAGCTCAAAC ACATTGTGTT TGTGGGATCC ATTGAGTACC TCAAGCGGGA ATGGGAGACA 
CTTCACAACT TCCCCAAAGT GTCCATATTG CCGGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCA 
GGAATGGACA GATCCTCTCC TGACAACAGC CCAGTGCACG GGATGTTACG GCAGCCATCC 
ATCACGACTG GGGTCAATAT CCCCATCATC ACAGAACTCG CTAAACCGGG CAAGTTGCCT 
TTGGTATCAG TCAATCAGGA AAAAAACAGT GGGACGCACA TTCTAATGAT AACGGAATTG 
GTGAATGACA CTAATGTTCA GTTTTTGGAC CAAGATGATG ACGATGACCC CGACACAGAG 
CTGTACCTCA CGCAACCCTT TGCGTGTGGG ACAGCTTTTG CCGTCAGCGT CCTGGACTCG 
CTCATGAGTG CGACATACTT CAATGACAAT ATCCTCACCC TGATACGGAC CTTGGTGACA 
GGAGGAGCCA CACCAGAGCT CGAGGCTCTG ATTGCTGAGG AGAATGCACT TCGAGGGGGC 
TACAGCACTC CCCAGACACT GGCCAACAGG GACCGTTGCA GAGTGGCCCA GTTAGCTCTG 
TTGGATGGGC CCTTTGCAGA CTTAGGGGAT GGTGGTTGTT ATGGTGATTT GTTCTGCAAA 
GCTCTGAAAA CATATAATAT GCTTTGTTTT GGAATTTACC GGCTGAGAGA TGCCCACCTC 
AGCACCCCCA GCCAGTGTAC GAAAAGGTAC GTCATCACCA ACCCCCCCTA CGAGTTTGAG 
CTGGTGCCGA CAGACCTGAT CTTCTGCCTG ATGCAGTTTG ACCACAACGC CGGCCAGTCC 
CGGGCCAGCC TGTCCCATTC CTCCCACTCA TCACAGTCCT CCAGCAAGAA GAGCTCCTCT 
GTCCACTCCA TCCCGTCCAC AGCAAACCGG CCAAACCGGC CCAAGTCCAG GGAGTCCCGC 
GACAAACAGA ACAGAAAAGA AATGGTTTAC AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848102.1
CDS	156..3791
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_008849880.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849880.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TTGAAGATGA ACAGCCGTCA ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC 
GGGGGCATGA GGAACTCGCC CAACTCCTCC CCGAAGCTGA TGAGGCATGA CCCCTTACTA 
ATTCCTGGCA ATGATCAGAT CGACAACATG GACTCCAATG TGAAGAAATA CGACTCTACA 
GGGATGTTTC ACTGGTGCGC AGCCAAGGAG ATCGAGAAAG TCATCCTGAC TCGAAGCGAA 
GCTGCCATGA CTGTCCTGAG TGGCCACGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA 
GCCCTGATTG GCCTCCGGAA CCTGGTGATG CCGCTTCGTG CCAGCAACTT CCACTACCAT 
GAGCTCAAAC ACATTGTGTT TGTGGGATCC ATTGAGTACC TCAAGCGGGA ATGGGAGACA 
CTTCACAACT TCCCCAAAGT GTCCATATTG CCGGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCA 
GGAATGGACA GATCCTCTCC TGACAACAGC CCAGTGCACG GGATGTTACG GCAGCCATCC 
ATCACGACTG GGGTCAATAT CCCCATCATC ACAGAACTCG CTAAACCGGG CAAGTTGCCT 
TTGGTATCAG TCAATCAGGA AAAAAACAGT GGGACGCACA TTCTAATGAT AACGGAATTG 
GTGAATGACA CTAATGTTCA GTTTTTGGAC CAAGATGATG ACGATGACCC CGACACAGAG 
CTGTACCTCA CGCAACCCTT TGCGTGTGGG ACAGCTTTTG CCGTCAGCGT CCTGGACTCG 
CTCATGAGTG CGACATACTT CAATGACAAT ATCCTCACCC TGATACGGAC CTTGGTGACA 
GGAGGAGCCA CACCAGAGCT CGAGGCTCTG ATTGCTGAGG AGAATGCACT TCGAGGGGGC 
TACAGCACTC CCCAGACACT GGCCAACAGG GACCGTTGCA GAGTGGCCCA GTTAGCTCTG 
TTGGATGGGC CCTTTGCAGA CTTAGGGGAT GGTGGTTGTT ATGGTGATTT GTTCTGCAAA 
GCTCTGAAAA CATATAATAT GCTTTGTTTT GGAATTTACC GGCTGAGAGA TGCCCACCTC 
AGCACCCCCA GCCAGTGTAC GAAAAGGTAC GTCATCACCA ACCCCCCCTA CGAGTTTGAG 
CTGGTGCCGA CAGACCTGAT CTTCTGCCTG ATGCAGTTTG ACCACAACGC CGGCCAGTCC 
CGGGCCAGCC TGTCCCATTC CTCCCACTCA TCACAGTCCT CCAGCAAGAA GAGCTCCTCT 
GTCCACTCCA TCCCGTCCAC AGCAAACCGG CCAAACCGGC CCAAGTCCAG GGAGTCCCGC 
GACAAACAGA ACAGAAAAGA AATGGTTTAC AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849881.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848103.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X25, mRNA.

Price & Availability↑

CloneID	ONa32298
Clone ID Related Accession (Same CDS sequence)	XM_008849881.2 , XM_008849881.3
Accession Version	XM_008849881.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TTGAAGATGA ACAGCCGTCA ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC 
GGGGGCATGA GGAACTCGCC CAACTCCTCC CCGAAGCTGA TGAGGCATGA CCCCTTACTA 
ATTCCTGGCA ATGATCAGAT CGACAACATG GACTCCAATG TGAAGAAATA CGACTCTACA 
GGGATGTTTC ACTGGTGCGC AGCCAAGGAG ATCGAGAAAG TCATCCTGAC TCGAAGCGAA 
GCTGCCATGA CTGTCCTGAG TGGCCACGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA 
GCCCTGATTG GCCTCCGGAA CCTGGTGATG CCGCTTCGTG CCAGCAACTT CCACTACCAT 
GAGCTCAAAC ACATTGTGTT TGTGGGATCC ATTGAGTACC TCAAGCGGGA ATGGGAGACA 
CTTCACAACT TCCCCAAAGT GTCCATATTG CCGGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCA 
GGAATGGACA GATCCTCTCC TGACAACAGC CCAGTGCACG GGATGTTACG GCAGCCATCC 
ATCACGACTG GGGTCAATAT CCCCATCATC ACAGAACTCG CTAAACCGGG CAAGTTGCCT 
TTGGTATCAG TCAATCAGGA AAAAAACAGT GGGACGCACA TTCTAATGAT AACGGAATTG 
GTGAATGACA CTAATGTTCA GTTTTTGGAC CAAGATGATG ACGATGACCC CGACACAGAG 
CTGTACCTCA CGCAACCCTT TGCGTGTGGG ACAGCTTTTG CCGTCAGCGT CCTGGACTCG 
CTCATGAGTG CGACATACTT CAATGACAAT ATCCTCACCC TGATACGGAC CTTGGTGACA 
GGAGGAGCCA CACCAGAGCT CGAGGCTCTG ATTGCTGAGG AGAATGCACT TCGAGGGGGC 
TACAGCACTC CCCAGACACT GGCCAACAGG GACCGTTGCA GAGTGGCCCA GTTAGCTCTG 
TTGGATGGGC CCTTTGCAGA CTTAGGGGAT GGTGGTTGTT ATGGTGATTT GTTCTGCAAA 
GCTCTGAAAA CATATAATAT GCTTTGTTTT GGAATTTACC GGCTGAGAGA TGCCCACCTC 
AGCACCCCCA GCCAGTGTAC GAAAAGGTAC GTCATCACCA ACCCCCCCTA CGAGTTTGAG 
CTGGTGCCGA CAGACCTGAT CTTCTGCCTG ATGCAGTTTG ACCACAACGC CGGCCAGTCC 
CGGGCCAGCC TGTCCCATTC CTCCCACTCA TCACAGTCCT CCAGCAAGAA GAGCTCCTCT 
GTCCACTCCA TCCCGTCCAC AGCAAACCGG CCAAACCGGC CCAAGTCCAG GGAGTCCCGC 
GACAAACAGA AAAAAGAAAT GGTTTACAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848103.1
CDS	156..3788
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR

Target ORF information:

RefSeq Version	XM_008849881.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849881.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TTGAAGATGA ACAGCCGTCA ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC 
GGGGGCATGA GGAACTCGCC CAACTCCTCC CCGAAGCTGA TGAGGCATGA CCCCTTACTA 
ATTCCTGGCA ATGATCAGAT CGACAACATG GACTCCAATG TGAAGAAATA CGACTCTACA 
GGGATGTTTC ACTGGTGCGC AGCCAAGGAG ATCGAGAAAG TCATCCTGAC TCGAAGCGAA 
GCTGCCATGA CTGTCCTGAG TGGCCACGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA 
GCCCTGATTG GCCTCCGGAA CCTGGTGATG CCGCTTCGTG CCAGCAACTT CCACTACCAT 
GAGCTCAAAC ACATTGTGTT TGTGGGATCC ATTGAGTACC TCAAGCGGGA ATGGGAGACA 
CTTCACAACT TCCCCAAAGT GTCCATATTG CCGGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCA 
GGAATGGACA GATCCTCTCC TGACAACAGC CCAGTGCACG GGATGTTACG GCAGCCATCC 
ATCACGACTG GGGTCAATAT CCCCATCATC ACAGAACTCG CTAAACCGGG CAAGTTGCCT 
TTGGTATCAG TCAATCAGGA AAAAAACAGT GGGACGCACA TTCTAATGAT AACGGAATTG 
GTGAATGACA CTAATGTTCA GTTTTTGGAC CAAGATGATG ACGATGACCC CGACACAGAG 
CTGTACCTCA CGCAACCCTT TGCGTGTGGG ACAGCTTTTG CCGTCAGCGT CCTGGACTCG 
CTCATGAGTG CGACATACTT CAATGACAAT ATCCTCACCC TGATACGGAC CTTGGTGACA 
GGAGGAGCCA CACCAGAGCT CGAGGCTCTG ATTGCTGAGG AGAATGCACT TCGAGGGGGC 
TACAGCACTC CCCAGACACT GGCCAACAGG GACCGTTGCA GAGTGGCCCA GTTAGCTCTG 
TTGGATGGGC CCTTTGCAGA CTTAGGGGAT GGTGGTTGTT ATGGTGATTT GTTCTGCAAA 
GCTCTGAAAA CATATAATAT GCTTTGTTTT GGAATTTACC GGCTGAGAGA TGCCCACCTC 
AGCACCCCCA GCCAGTGTAC GAAAAGGTAC GTCATCACCA ACCCCCCCTA CGAGTTTGAG 
CTGGTGCCGA CAGACCTGAT CTTCTGCCTG ATGCAGTTTG ACCACAACGC CGGCCAGTCC 
CGGGCCAGCC TGTCCCATTC CTCCCACTCA TCACAGTCCT CCAGCAAGAA GAGCTCCTCT 
GTCCACTCCA TCCCGTCCAC AGCAAACCGG CCAAACCGGC CCAAGTCCAG GGAGTCCCGC 
GACAAACAGA AAAAAGAAAT GGTTTACAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849881.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848103.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X17, mRNA.

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CloneID	ONa32298
Clone ID Related Accession (Same CDS sequence)	XM_008849881.2 , XM_008849881.3
Accession Version	XM_008849881.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008849881.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TTGAAGATGA ACAGCCGTCA ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC 
GGGGGCATGA GGAACTCGCC CAACTCCTCC CCGAAGCTGA TGAGGCATGA CCCCTTACTA 
ATTCCTGGCA ATGATCAGAT CGACAACATG GACTCCAATG TGAAGAAATA CGACTCTACA 
GGGATGTTTC ACTGGTGCGC AGCCAAGGAG ATCGAGAAAG TCATCCTGAC TCGAAGCGAA 
GCTGCCATGA CTGTCCTGAG TGGCCACGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA 
GCCCTGATTG GCCTCCGGAA CCTGGTGATG CCGCTTCGTG CCAGCAACTT CCACTACCAT 
GAGCTCAAAC ACATTGTGTT TGTGGGATCC ATTGAGTACC TCAAGCGGGA ATGGGAGACA 
CTTCACAACT TCCCCAAAGT GTCCATATTG CCGGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCA 
GGAATGGACA GATCCTCTCC TGACAACAGC CCAGTGCACG GGATGTTACG GCAGCCATCC 
ATCACGACTG GGGTCAATAT CCCCATCATC ACAGAACTCG CTAAACCGGG CAAGTTGCCT 
TTGGTATCAG TCAATCAGGA AAAAAACAGT GGGACGCACA TTCTAATGAT AACGGAATTG 
GTGAATGACA CTAATGTTCA GTTTTTGGAC CAAGATGATG ACGATGACCC CGACACAGAG 
CTGTACCTCA CGCAACCCTT TGCGTGTGGG ACAGCTTTTG CCGTCAGCGT CCTGGACTCG 
CTCATGAGTG CGACATACTT CAATGACAAT ATCCTCACCC TGATACGGAC CTTGGTGACA 
GGAGGAGCCA CACCAGAGCT CGAGGCTCTG ATTGCTGAGG AGAATGCACT TCGAGGGGGC 
TACAGCACTC CCCAGACACT GGCCAACAGG GACCGTTGCA GAGTGGCCCA GTTAGCTCTG 
TTGGATGGGC CCTTTGCAGA CTTAGGGGAT GGTGGTTGTT ATGGTGATTT GTTCTGCAAA 
GCTCTGAAAA CATATAATAT GCTTTGTTTT GGAATTTACC GGCTGAGAGA TGCCCACCTC 
AGCACCCCCA GCCAGTGTAC GAAAAGGTAC GTCATCACCA ACCCCCCCTA CGAGTTTGAG 
CTGGTGCCGA CAGACCTGAT CTTCTGCCTG ATGCAGTTTG ACCACAACGC CGGCCAGTCC 
CGGGCCAGCC TGTCCCATTC CTCCCACTCA TCACAGTCCT CCAGCAAGAA GAGCTCCTCT 
GTCCACTCCA TCCCGTCCAC AGCAAACCGG CCAAACCGGC CCAAGTCCAG GGAGTCCCGC 
GACAAACAGA AAAAAGAAAT GGTTTACAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848103.1
CDS	156..3788
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR

Target ORF information:

RefSeq Version	XM_008849881.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849881.3

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TTGAAGATGA ACAGCCGTCA ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC 
GGGGGCATGA GGAACTCGCC CAACTCCTCC CCGAAGCTGA TGAGGCATGA CCCCTTACTA 
ATTCCTGGCA ATGATCAGAT CGACAACATG GACTCCAATG TGAAGAAATA CGACTCTACA 
GGGATGTTTC ACTGGTGCGC AGCCAAGGAG ATCGAGAAAG TCATCCTGAC TCGAAGCGAA 
GCTGCCATGA CTGTCCTGAG TGGCCACGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA 
GCCCTGATTG GCCTCCGGAA CCTGGTGATG CCGCTTCGTG CCAGCAACTT CCACTACCAT 
GAGCTCAAAC ACATTGTGTT TGTGGGATCC ATTGAGTACC TCAAGCGGGA ATGGGAGACA 
CTTCACAACT TCCCCAAAGT GTCCATATTG CCGGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCA 
GGAATGGACA GATCCTCTCC TGACAACAGC CCAGTGCACG GGATGTTACG GCAGCCATCC 
ATCACGACTG GGGTCAATAT CCCCATCATC ACAGAACTCG CTAAACCGGG CAAGTTGCCT 
TTGGTATCAG TCAATCAGGA AAAAAACAGT GGGACGCACA TTCTAATGAT AACGGAATTG 
GTGAATGACA CTAATGTTCA GTTTTTGGAC CAAGATGATG ACGATGACCC CGACACAGAG 
CTGTACCTCA CGCAACCCTT TGCGTGTGGG ACAGCTTTTG CCGTCAGCGT CCTGGACTCG 
CTCATGAGTG CGACATACTT CAATGACAAT ATCCTCACCC TGATACGGAC CTTGGTGACA 
GGAGGAGCCA CACCAGAGCT CGAGGCTCTG ATTGCTGAGG AGAATGCACT TCGAGGGGGC 
TACAGCACTC CCCAGACACT GGCCAACAGG GACCGTTGCA GAGTGGCCCA GTTAGCTCTG 
TTGGATGGGC CCTTTGCAGA CTTAGGGGAT GGTGGTTGTT ATGGTGATTT GTTCTGCAAA 
GCTCTGAAAA CATATAATAT GCTTTGTTTT GGAATTTACC GGCTGAGAGA TGCCCACCTC 
AGCACCCCCA GCCAGTGTAC GAAAAGGTAC GTCATCACCA ACCCCCCCTA CGAGTTTGAG 
CTGGTGCCGA CAGACCTGAT CTTCTGCCTG ATGCAGTTTG ACCACAACGC CGGCCAGTCC 
CGGGCCAGCC TGTCCCATTC CTCCCACTCA TCACAGTCCT CCAGCAAGAA GAGCTCCTCT 
GTCCACTCCA TCCCGTCCAC AGCAAACCGG CCAAACCGGC CCAAGTCCAG GGAGTCCCGC 
GACAAACAGA AAAAAGAAAT GGTTTACAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849882.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848104.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X26, mRNA.

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CloneID	ONa32299
Clone ID Related Accession (Same CDS sequence)	XM_008849882.2 , XM_008849882.3
Accession Version	XM_008849882.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TTGAAGATGA ACAGCCGTCA ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC 
GGGGGCATGA GGAACTCGCC CAACTCCTCC CCGAAGCTGA TGAGGCATGA CCCCTTACTA 
ATTCCTGGCA ATGATCAGAT CGACAACATG GACTCCAATG TGAAGAAATA CGACTCTACA 
GGGATGTTTC ACTGGTGCGC AGCCAAGGAG ATCGAGAAAG TCATCCTGAC TCGAAGCGAA 
GCTGCCATGA CTGTCCTGAG TGGCCACGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA 
GCCCTGATTG GCCTCCGGAA CCTGGTGATG CCGCTTCGTG CCAGCAACTT CCACTACCAT 
GAGCTCAAAC ACATTGTGTT TGTGGGATCC ATTGAGTACC TCAAGCGGGA ATGGGAGACA 
CTTCACAACT TCCCCAAAGT GTCCATATTG CCGGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCA 
GGAATGGACA GATCCTCTCC TGACAACAGC CCAGTGCACG GGATGTTACG GCAGCCATCC 
ATCACGACTG GGGTCAATAT CCCCATCATC ACAGAACTCG TGAATGACAC TAATGTTCAG 
TTTTTGGACC AAGATGATGA CGATGACCCC GACACAGAGC TGTACCTCAC GCAACCCTTT 
GCGTGTGGGA CAGCTTTTGC CGTCAGCGTC CTGGACTCGC TCATGAGTGC GACATACTTC 
AATGACAATA TCCTCACCCT GATACGGACC TTGGTGACAG GAGGAGCCAC ACCAGAGCTC 
GAGGCTCTGA TTGCTGAGGA GAATGCACTT CGAGGGGGCT ACAGCACTCC CCAGACACTG 
GCCAACAGGG ACCGTTGCAG AGTGGCCCAG TTAGCTCTGT TGGATGGGCC CTTTGCAGAC 
TTAGGGGATG GTGGTTGTTA TGGTGATTTG TTCTGCAAAG CTCTGAAAAC ATATAATATG 
CTTTGTTTTG GAATTTACCG GCTGAGAGAT GCCCACCTCA GCACCCCCAG CCAGTGTACG 
AAAAGGTACG TCATCACCAA CCCCCCCTAC GAGTTTGAGC TGGTGCCGAC AGACCTGATC 
TTCTGCCTGA TGCAGTTTGA CCACAACGCC GGCCAGTCCC GGGCCAGCCT GTCCCATTCC 
TCCCACTCAT CACAGTCCTC CAGCAAGAAG AGCTCCTCTG TCCACTCCAT CCCGTCCACA 
GCAAACCGGC CAAACCGGCC CAAGTCCAGG GAGTCCCGCG ACAAACAGAA CAGAAAAGAA 
ATGGTTTACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848104.1
CDS	156..3710
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_008849882.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849882.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TTGAAGATGA ACAGCCGTCA ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC 
GGGGGCATGA GGAACTCGCC CAACTCCTCC CCGAAGCTGA TGAGGCATGA CCCCTTACTA 
ATTCCTGGCA ATGATCAGAT CGACAACATG GACTCCAATG TGAAGAAATA CGACTCTACA 
GGGATGTTTC ACTGGTGCGC AGCCAAGGAG ATCGAGAAAG TCATCCTGAC TCGAAGCGAA 
GCTGCCATGA CTGTCCTGAG TGGCCACGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA 
GCCCTGATTG GCCTCCGGAA CCTGGTGATG CCGCTTCGTG CCAGCAACTT CCACTACCAT 
GAGCTCAAAC ACATTGTGTT TGTGGGATCC ATTGAGTACC TCAAGCGGGA ATGGGAGACA 
CTTCACAACT TCCCCAAAGT GTCCATATTG CCGGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCA 
GGAATGGACA GATCCTCTCC TGACAACAGC CCAGTGCACG GGATGTTACG GCAGCCATCC 
ATCACGACTG GGGTCAATAT CCCCATCATC ACAGAACTCG TGAATGACAC TAATGTTCAG 
TTTTTGGACC AAGATGATGA CGATGACCCC GACACAGAGC TGTACCTCAC GCAACCCTTT 
GCGTGTGGGA CAGCTTTTGC CGTCAGCGTC CTGGACTCGC TCATGAGTGC GACATACTTC 
AATGACAATA TCCTCACCCT GATACGGACC TTGGTGACAG GAGGAGCCAC ACCAGAGCTC 
GAGGCTCTGA TTGCTGAGGA GAATGCACTT CGAGGGGGCT ACAGCACTCC CCAGACACTG 
GCCAACAGGG ACCGTTGCAG AGTGGCCCAG TTAGCTCTGT TGGATGGGCC CTTTGCAGAC 
TTAGGGGATG GTGGTTGTTA TGGTGATTTG TTCTGCAAAG CTCTGAAAAC ATATAATATG 
CTTTGTTTTG GAATTTACCG GCTGAGAGAT GCCCACCTCA GCACCCCCAG CCAGTGTACG 
AAAAGGTACG TCATCACCAA CCCCCCCTAC GAGTTTGAGC TGGTGCCGAC AGACCTGATC 
TTCTGCCTGA TGCAGTTTGA CCACAACGCC GGCCAGTCCC GGGCCAGCCT GTCCCATTCC 
TCCCACTCAT CACAGTCCTC CAGCAAGAAG AGCTCCTCTG TCCACTCCAT CCCGTCCACA 
GCAAACCGGC CAAACCGGCC CAAGTCCAGG GAGTCCCGCG ACAAACAGAA CAGAAAAGAA 
ATGGTTTACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849882.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848104.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	ONa32299
Clone ID Related Accession (Same CDS sequence)	XM_008849882.2 , XM_008849882.3
Accession Version	XM_008849882.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008849882.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TTGAAGATGA ACAGCCGTCA ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC 
GGGGGCATGA GGAACTCGCC CAACTCCTCC CCGAAGCTGA TGAGGCATGA CCCCTTACTA 
ATTCCTGGCA ATGATCAGAT CGACAACATG GACTCCAATG TGAAGAAATA CGACTCTACA 
GGGATGTTTC ACTGGTGCGC AGCCAAGGAG ATCGAGAAAG TCATCCTGAC TCGAAGCGAA 
GCTGCCATGA CTGTCCTGAG TGGCCACGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA 
GCCCTGATTG GCCTCCGGAA CCTGGTGATG CCGCTTCGTG CCAGCAACTT CCACTACCAT 
GAGCTCAAAC ACATTGTGTT TGTGGGATCC ATTGAGTACC TCAAGCGGGA ATGGGAGACA 
CTTCACAACT TCCCCAAAGT GTCCATATTG CCGGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCA 
GGAATGGACA GATCCTCTCC TGACAACAGC CCAGTGCACG GGATGTTACG GCAGCCATCC 
ATCACGACTG GGGTCAATAT CCCCATCATC ACAGAACTCG TGAATGACAC TAATGTTCAG 
TTTTTGGACC AAGATGATGA CGATGACCCC GACACAGAGC TGTACCTCAC GCAACCCTTT 
GCGTGTGGGA CAGCTTTTGC CGTCAGCGTC CTGGACTCGC TCATGAGTGC GACATACTTC 
AATGACAATA TCCTCACCCT GATACGGACC TTGGTGACAG GAGGAGCCAC ACCAGAGCTC 
GAGGCTCTGA TTGCTGAGGA GAATGCACTT CGAGGGGGCT ACAGCACTCC CCAGACACTG 
GCCAACAGGG ACCGTTGCAG AGTGGCCCAG TTAGCTCTGT TGGATGGGCC CTTTGCAGAC 
TTAGGGGATG GTGGTTGTTA TGGTGATTTG TTCTGCAAAG CTCTGAAAAC ATATAATATG 
CTTTGTTTTG GAATTTACCG GCTGAGAGAT GCCCACCTCA GCACCCCCAG CCAGTGTACG 
AAAAGGTACG TCATCACCAA CCCCCCCTAC GAGTTTGAGC TGGTGCCGAC AGACCTGATC 
TTCTGCCTGA TGCAGTTTGA CCACAACGCC GGCCAGTCCC GGGCCAGCCT GTCCCATTCC 
TCCCACTCAT CACAGTCCTC CAGCAAGAAG AGCTCCTCTG TCCACTCCAT CCCGTCCACA 
GCAAACCGGC CAAACCGGCC CAAGTCCAGG GAGTCCCGCG ACAAACAGAA CAGAAAAGAA 
ATGGTTTACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848104.1
CDS	156..3710
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_008849882.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849882.3

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAACC CTGGGAACCA TCTTAAGATC 
CAAGAAGGCA CTTTAGGATT TTTCATCGCG AGTGATGCCA AGGAAGTTAA AAGGGCATTT 
TTTTACTGCA AGGCCTGTCA TGATGACGTC ACAGACCCCA AAAGAATTAA AAAATGCGGC 
TGCAGACGGC TTGAAGATGA ACAGCCGTCA ACGCTGTCAC CCAAAAAAAA ACAGCGCAAC 
GGGGGCATGA GGAACTCGCC CAACTCCTCC CCGAAGCTGA TGAGGCATGA CCCCTTACTA 
ATTCCTGGCA ATGATCAGAT CGACAACATG GACTCCAATG TGAAGAAATA CGACTCTACA 
GGGATGTTTC ACTGGTGCGC AGCCAAGGAG ATCGAGAAAG TCATCCTGAC TCGAAGCGAA 
GCTGCCATGA CTGTCCTGAG TGGCCACGTC GTAGTCTGCA TCTTTGGGGA TGTCAGCTCA 
GCCCTGATTG GCCTCCGGAA CCTGGTGATG CCGCTTCGTG CCAGCAACTT CCACTACCAT 
GAGCTCAAAC ACATTGTGTT TGTGGGATCC ATTGAGTACC TCAAGCGGGA ATGGGAGACA 
CTTCACAACT TCCCCAAAGT GTCCATATTG CCGGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGATT CACACCTCCA 
GGAATGGACA GATCCTCTCC TGACAACAGC CCAGTGCACG GGATGTTACG GCAGCCATCC 
ATCACGACTG GGGTCAATAT CCCCATCATC ACAGAACTCG TGAATGACAC TAATGTTCAG 
TTTTTGGACC AAGATGATGA CGATGACCCC GACACAGAGC TGTACCTCAC GCAACCCTTT 
GCGTGTGGGA CAGCTTTTGC CGTCAGCGTC CTGGACTCGC TCATGAGTGC GACATACTTC 
AATGACAATA TCCTCACCCT GATACGGACC TTGGTGACAG GAGGAGCCAC ACCAGAGCTC 
GAGGCTCTGA TTGCTGAGGA GAATGCACTT CGAGGGGGCT ACAGCACTCC CCAGACACTG 
GCCAACAGGG ACCGTTGCAG AGTGGCCCAG TTAGCTCTGT TGGATGGGCC CTTTGCAGAC 
TTAGGGGATG GTGGTTGTTA TGGTGATTTG TTCTGCAAAG CTCTGAAAAC ATATAATATG 
CTTTGTTTTG GAATTTACCG GCTGAGAGAT GCCCACCTCA GCACCCCCAG CCAGTGTACG 
AAAAGGTACG TCATCACCAA CCCCCCCTAC GAGTTTGAGC TGGTGCCGAC AGACCTGATC 
TTCTGCCTGA TGCAGTTTGA CCACAACGCC GGCCAGTCCC GGGCCAGCCT GTCCCATTCC 
TCCCACTCAT CACAGTCCTC CAGCAAGAAG AGCTCCTCTG TCCACTCCAT CCCGTCCACA 
GCAAACCGGC CAAACCGGCC CAAGTCCAGG GAGTCCCGCG ACAAACAGAA CAGAAAAGAA 
ATGGTTTACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849883.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848105.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X19, mRNA.

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CloneID	ONa32300
Clone ID Related Accession (Same CDS sequence)	XM_008849883.2
Accession Version	XM_008849883.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTT 
GAAGATGAAC AGCCGTCAAC GCTGTCACCC AAAAAAAAAC AGCGCAACGG GGGCATGAGG 
AACTCGCCCA ACTCCTCCCC GAAGCTGATG AGGCATGACC CCTTACTAAT TCCTGGCAAT 
GATCAGATCG ACAACATGGA CTCCAATGTG AAGAAATACG ACTCTACAGG GATGTTTCAC 
TGGTGCGCAG CCAAGGAGAT CGAGAAAGTC ATCCTGACTC GAAGCGAAGC TGCCATGACT 
GTCCTGAGTG GCCACGTCGT AGTCTGCATC TTTGGGGATG TCAGCTCAGC CCTGATTGGC 
CTCCGGAACC TGGTGATGCC GCTTCGTGCC AGCAACTTCC ACTACCATGA GCTCAAACAC 
ATTGTGTTTG TGGGATCCAT TGAGTACCTC AAGCGGGAAT GGGAGACACT TCACAACTTC 
CCCAAAGTGT CCATATTGCC GGGTACGCCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATACT 
TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA CACCTCCAGG AATGGACAGA 
TCCTCTCCTG ACAACAGCCC AGTGCACGGG ATGTTACGGC AGCCATCCAT CACGACTGGG 
GTCAATATCC CCATCATCAC AGAACTCGTG AATGACACTA ATGTTCAGTT TTTGGACCAA 
GATGATGACG ATGACCCCGA CACAGAGCTG TACCTCACGC AACCCTTTGC GTGTGGGACA 
GCTTTTGCCG TCAGCGTCCT GGACTCGCTC ATGAGTGCGA CATACTTCAA TGACAATATC 
CTCACCCTGA TACGGACCTT GGTGACAGGA GGAGCCACAC CAGAGCTCGA GGCTCTGATT 
GCTGAGGAGA ATGCACTTCG AGGGGGCTAC AGCACTCCCC AGACACTGGC CAACAGGGAC 
CGTTGCAGAG TGGCCCAGTT AGCTCTGTTG GATGGGCCCT TTGCAGACTT AGGGGATGGT 
GGTTGTTATG GTGATTTGTT CTGCAAAGCT CTGAAAACAT ATAATATGCT TTGTTTTGGA 
ATTTACCGGC TGAGAGATGC CCACCTCAGC ACCCCCAGCC AGTGTACGAA AAGGTACGTC 
ATCACCAACC CCCCCTACGA GTTTGAGCTG GTGCCGACAG ACCTGATCTT CTGCCTGATG 
CAGTTTGACC ACAACGCCGG CCAGTCCCGG GCCAGCCTGT CCCATTCCTC CCACTCATCA 
CAGTCCTCCA GCAAGAAGAG CTCCTCTGTC CACTCCATCC CGTCCACAGC AAACCGGCCA 
AACCGGCCCA AGTCCAGGGA GTCCCGCGAC AAACAGAACA GAAAAGAAAT GGTTTACAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848105.1
CDS	156..3698
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_008849883.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849883.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTT 
GAAGATGAAC AGCCGTCAAC GCTGTCACCC AAAAAAAAAC AGCGCAACGG GGGCATGAGG 
AACTCGCCCA ACTCCTCCCC GAAGCTGATG AGGCATGACC CCTTACTAAT TCCTGGCAAT 
GATCAGATCG ACAACATGGA CTCCAATGTG AAGAAATACG ACTCTACAGG GATGTTTCAC 
TGGTGCGCAG CCAAGGAGAT CGAGAAAGTC ATCCTGACTC GAAGCGAAGC TGCCATGACT 
GTCCTGAGTG GCCACGTCGT AGTCTGCATC TTTGGGGATG TCAGCTCAGC CCTGATTGGC 
CTCCGGAACC TGGTGATGCC GCTTCGTGCC AGCAACTTCC ACTACCATGA GCTCAAACAC 
ATTGTGTTTG TGGGATCCAT TGAGTACCTC AAGCGGGAAT GGGAGACACT TCACAACTTC 
CCCAAAGTGT CCATATTGCC GGGTACGCCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATACT 
TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA CACCTCCAGG AATGGACAGA 
TCCTCTCCTG ACAACAGCCC AGTGCACGGG ATGTTACGGC AGCCATCCAT CACGACTGGG 
GTCAATATCC CCATCATCAC AGAACTCGTG AATGACACTA ATGTTCAGTT TTTGGACCAA 
GATGATGACG ATGACCCCGA CACAGAGCTG TACCTCACGC AACCCTTTGC GTGTGGGACA 
GCTTTTGCCG TCAGCGTCCT GGACTCGCTC ATGAGTGCGA CATACTTCAA TGACAATATC 
CTCACCCTGA TACGGACCTT GGTGACAGGA GGAGCCACAC CAGAGCTCGA GGCTCTGATT 
GCTGAGGAGA ATGCACTTCG AGGGGGCTAC AGCACTCCCC AGACACTGGC CAACAGGGAC 
CGTTGCAGAG TGGCCCAGTT AGCTCTGTTG GATGGGCCCT TTGCAGACTT AGGGGATGGT 
GGTTGTTATG GTGATTTGTT CTGCAAAGCT CTGAAAACAT ATAATATGCT TTGTTTTGGA 
ATTTACCGGC TGAGAGATGC CCACCTCAGC ACCCCCAGCC AGTGTACGAA AAGGTACGTC 
ATCACCAACC CCCCCTACGA GTTTGAGCTG GTGCCGACAG ACCTGATCTT CTGCCTGATG 
CAGTTTGACC ACAACGCCGG CCAGTCCCGG GCCAGCCTGT CCCATTCCTC CCACTCATCA 
CAGTCCTCCA GCAAGAAGAG CTCCTCTGTC CACTCCATCC CGTCCACAGC AAACCGGCCA 
AACCGGCCCA AGTCCAGGGA GTCCCGCGAC AAACAGAACA GAAAAGAAAT GGTTTACAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849885.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848107.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X20, mRNA.

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CloneID	ONa32301
Clone ID Related Accession (Same CDS sequence)	XM_008849885.2
Accession Version	XM_008849885.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTT 
GAAGATGAAC AGCCGTCAAC GCTGTCACCC AAAAAAAAAC AGCGCAACGG GGGCATGAGG 
AACTCGCCCA ACTCCTCCCC GAAGCTGATG AGGCATGACC CCTTACTAAT TCCTGGCAAT 
GATCAGATCG ACAACATGGA CTCCAATGTG AAGAAATACG ACTCTACAGG GATGTTTCAC 
TGGTGCGCAG CCAAGGAGAT CGAGAAAGTC ATCCTGACTC GAAGCGAAGC TGCCATGACT 
GTCCTGAGTG GCCACGTCGT AGTCTGCATC TTTGGGGATG TCAGCTCAGC CCTGATTGGC 
CTCCGGAACC TGGTGATGCC GCTTCGTGCC AGCAACTTCC ACTACCATGA GCTCAAACAC 
ATTGTGTTTG TGGGATCCAT TGAGTACCTC AAGCGGGAAT GGGAGACACT TCACAACTTC 
CCCAAAGTGT CCATATTGCC GGGTACGCCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATACT 
TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA CACCTCCAGG AATGGACAGA 
TCCTCTCCTG ACAACAGCCC AGTGCACGGG ATGTTACGGC AGCCATCCAT CACGACTGGG 
GTCAATATCC CCATCATCAC AGAACTCGTG AATGACACTA ATGTTCAGTT TTTGGACCAA 
GATGATGACG ATGACCCCGA CACAGAGCTG TACCTCACGC AACCCTTTGC GTGTGGGACA 
GCTTTTGCCG TCAGCGTCCT GGACTCGCTC ATGAGTGCGA CATACTTCAA TGACAATATC 
CTCACCCTGA TACGGACCTT GGTGACAGGA GGAGCCACAC CAGAGCTCGA GGCTCTGATT 
GCTGAGGAGA ATGCACTTCG AGGGGGCTAC AGCACTCCCC AGACACTGGC CAACAGGGAC 
CGTTGCAGAG TGGCCCAGTT AGCTCTGTTG GATGGGCCCT TTGCAGACTT AGGGGATGGT 
GGTTGTTATG GTGATTTGTT CTGCAAAGCT CTGAAAACAT ATAATATGCT TTGTTTTGGA 
ATTTACCGGC TGAGAGATGC CCACCTCAGC ACCCCCAGCC AGTGTACGAA AAGGTACGTC 
ATCACCAACC CCCCCTACGA GTTTGAGCTG GTGCCGACAG ACCTGATCTT CTGCCTGATG 
CAGTTTGACC ACAACGCCGG CCAGTCCCGG GCCAGCCTGT CCCATTCCTC CCACTCATCA 
CAGTCCTCCA GCAAGAAGAG CTCCTCTGTC CACTCCATCC CGTCCACAGC AAACCGGCCA 
AACCGGCCCA AGTCCAGGGA GTCCCGCGAC AAACAGAAAA AAGAAATGGT TTACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848107.1
CDS	156..3695
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR

Target ORF information:

RefSeq Version	XM_008849885.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849885.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTT 
GAAGATGAAC AGCCGTCAAC GCTGTCACCC AAAAAAAAAC AGCGCAACGG GGGCATGAGG 
AACTCGCCCA ACTCCTCCCC GAAGCTGATG AGGCATGACC CCTTACTAAT TCCTGGCAAT 
GATCAGATCG ACAACATGGA CTCCAATGTG AAGAAATACG ACTCTACAGG GATGTTTCAC 
TGGTGCGCAG CCAAGGAGAT CGAGAAAGTC ATCCTGACTC GAAGCGAAGC TGCCATGACT 
GTCCTGAGTG GCCACGTCGT AGTCTGCATC TTTGGGGATG TCAGCTCAGC CCTGATTGGC 
CTCCGGAACC TGGTGATGCC GCTTCGTGCC AGCAACTTCC ACTACCATGA GCTCAAACAC 
ATTGTGTTTG TGGGATCCAT TGAGTACCTC AAGCGGGAAT GGGAGACACT TCACAACTTC 
CCCAAAGTGT CCATATTGCC GGGTACGCCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATACT 
TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA CACCTCCAGG AATGGACAGA 
TCCTCTCCTG ACAACAGCCC AGTGCACGGG ATGTTACGGC AGCCATCCAT CACGACTGGG 
GTCAATATCC CCATCATCAC AGAACTCGTG AATGACACTA ATGTTCAGTT TTTGGACCAA 
GATGATGACG ATGACCCCGA CACAGAGCTG TACCTCACGC AACCCTTTGC GTGTGGGACA 
GCTTTTGCCG TCAGCGTCCT GGACTCGCTC ATGAGTGCGA CATACTTCAA TGACAATATC 
CTCACCCTGA TACGGACCTT GGTGACAGGA GGAGCCACAC CAGAGCTCGA GGCTCTGATT 
GCTGAGGAGA ATGCACTTCG AGGGGGCTAC AGCACTCCCC AGACACTGGC CAACAGGGAC 
CGTTGCAGAG TGGCCCAGTT AGCTCTGTTG GATGGGCCCT TTGCAGACTT AGGGGATGGT 
GGTTGTTATG GTGATTTGTT CTGCAAAGCT CTGAAAACAT ATAATATGCT TTGTTTTGGA 
ATTTACCGGC TGAGAGATGC CCACCTCAGC ACCCCCAGCC AGTGTACGAA AAGGTACGTC 
ATCACCAACC CCCCCTACGA GTTTGAGCTG GTGCCGACAG ACCTGATCTT CTGCCTGATG 
CAGTTTGACC ACAACGCCGG CCAGTCCCGG GCCAGCCTGT CCCATTCCTC CCACTCATCA 
CAGTCCTCCA GCAAGAAGAG CTCCTCTGTC CACTCCATCC CGTCCACAGC AAACCGGCCA 
AACCGGCCCA AGTCCAGGGA GTCCCGCGAC AAACAGAAAA AAGAAATGGT TTACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849887.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848109.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X29, mRNA.

Price & Availability↑

CloneID	ONa32302
Clone ID Related Accession (Same CDS sequence)	XM_008849887.2
Accession Version	XM_008849887.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTG 
ATCTATTCCA AGACGTCCAT CTACAAGAGA ATGAGCCGAG CATGTTGTTT TGATTGCGGA 
CGTTCTGAGC GTGACTGCTC ATGCATGTCA GGCCGTGTGC GTGGTAACGT GGACACCCCT 
GAGAGAACCT TCCCGCTTTC TTCTGTCTCT GTTAATGATT GCGCCACCAG TTTCCGTGCC 
TTTGAAGATG AACAGCCGTC AACGCTGTCA CCCAAAAAAA AACAGCGCAA CGGGGGCATG 
AGGAACTCGC CCAACTCCTC CCCGAAGCTG ATGAGGCATG ACCCCTTACT AATTCCTGGC 
AATGATCAGA TCGACAACAT GGACTCCAAT GTGAAGAAAT ACGACTCTAC AGGGATGTTT 
CACTGGTGCG CAGCCAAGGA GATCGAGAAA GTCATCCTGA CTCGAAGCGA AGCTGCCATG 
ACTGTCCTGA GTGGCCACGT CGTAGTCTGC ATCTTTGGGG ATGTCAGCTC AGCCCTGATT 
GGCCTCCGGA ACCTGGTGAT GCCGCTTCGT GCCAGCAACT TCCACTACCA TGAGCTCAAA 
CACATTGTGT TTGTGGGATC CATTGAGTAC CTCAAGCGGG AATGGGAGAC ACTTCACAAC 
TTCCCCAAAG TGTCCATATT GCCGGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGTGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
ACTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TCGGGGTCTT GCAGGCTAAT TCCCAAGGAT TCACACCTCC AGGAATGGAC 
AGATCCTCTC CTGACAACAG CCCAGTGCAC GGGATGTTAC GGCAGCCATC CATCACGACT 
GGGGTCAATA TCCCCATCAT CACAGAACTC GCTAAACCGG GCAAGTTGCC TTTGGTATCA 
GTCAATCAGG AAAAAAACAG TGGGACGCAC ATTCTAATGA TAACGGAATT GGTGAATGAC 
ACTAATGTTC AGTTTTTGGA CCAAGATGAT GACGATGACC CCGACACAGA GCTGTACCTC 
ACGCAACCCT TTGCGTGTGG GACAGCTTTT GCCGTCAGCG TCCTGGACTC GCTCATGAGT 
GCGACATACT TCAATGACAA TATCCTCACC CTGATACGGA CCTTGGTGAC AGGAGGAGCC 
ACACCAGAGC TCGAGGCTCT GATTGCTGAG GAGAATGCAC TTCGAGGGGG CTACAGCACT 
CCCCAGACAC TGGCCAACAG GGACCGTTGC AGAGTGGCCC AGTTAGCTCT GTTGGATGGG 
CCCTTTGCAG ACTTAGGGGA TGGTGGTTGT TATGGTGATT TGTTCTGCAA AGCTCTGAAA 
ACATATAATA TGCTTTGTTT TGGAATTTAC CGGCTGAGAG ATGCCCACCT CAGCACCCCC 
AGCCAGTGTA CGAAAAGGTA CGTCATCACC AACCCCCCCT ACGAGTTTGA GCTGGTGCCG 
ACAGACCTGA TCTTCTGCCT GATGCAGTTT GACCACAACG CCGGCCAGTC CCGGGCCAGC 
CTGTCCCATT CCTCCCACTC ATCACAGTCC TCCAGCAAGA AGAGCTCCTC TGTCCACTCC 
ATCCCGTCCA CAGCAAACCG GCCAAACCGG CCCAAGTCCA GGGAGTCCCG CGACAAACAG 
AAAAAAGAAA TGGTTTACAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848109.1
CDS	156..3959
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIYSKTSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTPERTFPLSSVSVNDCATSFRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR

Target ORF information:

RefSeq Version	XM_008849887.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849887.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTG 
ATCTATTCCA AGACGTCCAT CTACAAGAGA ATGAGCCGAG CATGTTGTTT TGATTGCGGA 
CGTTCTGAGC GTGACTGCTC ATGCATGTCA GGCCGTGTGC GTGGTAACGT GGACACCCCT 
GAGAGAACCT TCCCGCTTTC TTCTGTCTCT GTTAATGATT GCGCCACCAG TTTCCGTGCC 
TTTGAAGATG AACAGCCGTC AACGCTGTCA CCCAAAAAAA AACAGCGCAA CGGGGGCATG 
AGGAACTCGC CCAACTCCTC CCCGAAGCTG ATGAGGCATG ACCCCTTACT AATTCCTGGC 
AATGATCAGA TCGACAACAT GGACTCCAAT GTGAAGAAAT ACGACTCTAC AGGGATGTTT 
CACTGGTGCG CAGCCAAGGA GATCGAGAAA GTCATCCTGA CTCGAAGCGA AGCTGCCATG 
ACTGTCCTGA GTGGCCACGT CGTAGTCTGC ATCTTTGGGG ATGTCAGCTC AGCCCTGATT 
GGCCTCCGGA ACCTGGTGAT GCCGCTTCGT GCCAGCAACT TCCACTACCA TGAGCTCAAA 
CACATTGTGT TTGTGGGATC CATTGAGTAC CTCAAGCGGG AATGGGAGAC ACTTCACAAC 
TTCCCCAAAG TGTCCATATT GCCGGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGTGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
ACTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TCGGGGTCTT GCAGGCTAAT TCCCAAGGAT TCACACCTCC AGGAATGGAC 
AGATCCTCTC CTGACAACAG CCCAGTGCAC GGGATGTTAC GGCAGCCATC CATCACGACT 
GGGGTCAATA TCCCCATCAT CACAGAACTC GCTAAACCGG GCAAGTTGCC TTTGGTATCA 
GTCAATCAGG AAAAAAACAG TGGGACGCAC ATTCTAATGA TAACGGAATT GGTGAATGAC 
ACTAATGTTC AGTTTTTGGA CCAAGATGAT GACGATGACC CCGACACAGA GCTGTACCTC 
ACGCAACCCT TTGCGTGTGG GACAGCTTTT GCCGTCAGCG TCCTGGACTC GCTCATGAGT 
GCGACATACT TCAATGACAA TATCCTCACC CTGATACGGA CCTTGGTGAC AGGAGGAGCC 
ACACCAGAGC TCGAGGCTCT GATTGCTGAG GAGAATGCAC TTCGAGGGGG CTACAGCACT 
CCCCAGACAC TGGCCAACAG GGACCGTTGC AGAGTGGCCC AGTTAGCTCT GTTGGATGGG 
CCCTTTGCAG ACTTAGGGGA TGGTGGTTGT TATGGTGATT TGTTCTGCAA AGCTCTGAAA 
ACATATAATA TGCTTTGTTT TGGAATTTAC CGGCTGAGAG ATGCCCACCT CAGCACCCCC 
AGCCAGTGTA CGAAAAGGTA CGTCATCACC AACCCCCCCT ACGAGTTTGA GCTGGTGCCG 
ACAGACCTGA TCTTCTGCCT GATGCAGTTT GACCACAACG CCGGCCAGTC CCGGGCCAGC 
CTGTCCCATT CCTCCCACTC ATCACAGTCC TCCAGCAAGA AGAGCTCCTC TGTCCACTCC 
ATCCCGTCCA CAGCAAACCG GCCAAACCGG CCCAAGTCCA GGGAGTCCCG CGACAAACAG 
AAAAAAGAAA TGGTTTACAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849888.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848110.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X30, mRNA.

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CloneID	ONa32303
Clone ID Related Accession (Same CDS sequence)	XM_008849888.1
Accession Version	XM_008849888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTC 
AAGGCTGCAG CTAGATCATG CTATCACAAA GACCCATTTG TGCACAAGAA CGCAACTCCC 
AAATCTCGGC TTGTGACTGA GCCAGTTGAA GATGAACAGC CGTCAACGCT GTCACCCAAA 
AAAAAACAGC GCAACGGGGG CATGAGGAAC TCGCCCAACT CCTCCCCGAA GCTGATGAGG 
CATGACCCCT TACTAATTCC TGGCAATGAT CAGATCGACA ACATGGACTC CAATGTGAAG 
AAATACGACT CTACAGGGAT GTTTCACTGG TGCGCAGCCA AGGAGATCGA GAAAGTCATC 
CTGACTCGAA GCGAAGCTGC CATGACTGTC CTGAGTGGCC ACGTCGTAGT CTGCATCTTT 
GGGGATGTCA GCTCAGCCCT GATTGGCCTC CGGAACCTGG TGATGCCGCT TCGTGCCAGC 
AACTTCCACT ACCATGAGCT CAAACACATT GTGTTTGTGG GATCCATTGA GTACCTCAAG 
CGGGAATGGG AGACACTTCA CAACTTCCCC AAAGTGTCCA TATTGCCGGG TACGCCATTA 
AGTCGGGCTG ATTTAAGGGC TGTCAACATC AACCTCTGTG ACATGTGCGT TATCCTGTCA 
GCCAATCAGA ATAATATTGA TGATACTTCG CTGCAGGACA AGGAATGCAT CTTGGCGTCA 
CTCAACATCA AATCTATGCA GTTTGATGAC AGCATCGGGG TCTTGCAGGC TAATTCCCAA 
GGATTCACAC CTCCAGGAAT GGACAGATCC TCTCCTGACA ACAGCCCAGT GCACGGGATG 
TTACGGCAGC CATCCATCAC GACTGGGGTC AATATCCCCA TCATCACAGA ACTCGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAAGTACG TTCAGGAAGA ACGGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848110.1
CDS	156..3785
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLKAAARSCYHKDPFVHKNATPKSRLVTEPVEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKYVQEERL

Target ORF information:

RefSeq Version	XM_008849888.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849888.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTC 
AAGGCTGCAG CTAGATCATG CTATCACAAA GACCCATTTG TGCACAAGAA CGCAACTCCC 
AAATCTCGGC TTGTGACTGA GCCAGTTGAA GATGAACAGC CGTCAACGCT GTCACCCAAA 
AAAAAACAGC GCAACGGGGG CATGAGGAAC TCGCCCAACT CCTCCCCGAA GCTGATGAGG 
CATGACCCCT TACTAATTCC TGGCAATGAT CAGATCGACA ACATGGACTC CAATGTGAAG 
AAATACGACT CTACAGGGAT GTTTCACTGG TGCGCAGCCA AGGAGATCGA GAAAGTCATC 
CTGACTCGAA GCGAAGCTGC CATGACTGTC CTGAGTGGCC ACGTCGTAGT CTGCATCTTT 
GGGGATGTCA GCTCAGCCCT GATTGGCCTC CGGAACCTGG TGATGCCGCT TCGTGCCAGC 
AACTTCCACT ACCATGAGCT CAAACACATT GTGTTTGTGG GATCCATTGA GTACCTCAAG 
CGGGAATGGG AGACACTTCA CAACTTCCCC AAAGTGTCCA TATTGCCGGG TACGCCATTA 
AGTCGGGCTG ATTTAAGGGC TGTCAACATC AACCTCTGTG ACATGTGCGT TATCCTGTCA 
GCCAATCAGA ATAATATTGA TGATACTTCG CTGCAGGACA AGGAATGCAT CTTGGCGTCA 
CTCAACATCA AATCTATGCA GTTTGATGAC AGCATCGGGG TCTTGCAGGC TAATTCCCAA 
GGATTCACAC CTCCAGGAAT GGACAGATCC TCTCCTGACA ACAGCCCAGT GCACGGGATG 
TTACGGCAGC CATCCATCAC GACTGGGGTC AATATCCCCA TCATCACAGA ACTCGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAAGTACG TTCAGGAAGA ACGGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_008849889.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008848111.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X31, mRNA.

Price & Availability↑

CloneID	ONa32304
Clone ID Related Accession (Same CDS sequence)	XM_008849889.2
Accession Version	XM_008849889.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008849889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTC 
AAGGCTGCAG CTAGATCATG CTATCACAAA GACCCATTTG TGCACAAGAA CGCAACTCCC 
AAATCTCGGC TTGTGACTGA GCCAGTTGAA GATGAACAGC CGTCAACGCT GTCACCCAAA 
AAAAAACAGC GCAACGGGGG CATGAGGAAC TCGCCCAACT CCTCCCCGAA GCTGATGAGG 
CATGACCCCT TACTAATTCC TGGCAATGAT CAGATCGACA ACATGGACTC CAATGTGAAG 
AAATACGACT CTACAGGGAT GTTTCACTGG TGCGCAGCCA AGGAGATCGA GAAAGTCATC 
CTGACTCGAA GCGAAGCTGC CATGACTGTC CTGAGTGGCC ACGTCGTAGT CTGCATCTTT 
GGGGATGTCA GCTCAGCCCT GATTGGCCTC CGGAACCTGG TGATGCCGCT TCGTGCCAGC 
AACTTCCACT ACCATGAGCT CAAACACATT GTGTTTGTGG GATCCATTGA GTACCTCAAG 
CGGGAATGGG AGACACTTCA CAACTTCCCC AAAGTGTCCA TATTGCCGGG TACGCCATTA 
AGTCGGGCTG ATTTAAGGGC TGTCAACATC AACCTCTGTG ACATGTGCGT TATCCTGTCA 
GCCAATCAGA ATAATATTGA TGATACTTCG CTGCAGGACA AGGAATGCAT CTTGGCGTCA 
CTCAACATCA AATCTATGCA GTTTGATGAC AGCATCGGGG TCTTGCAGGC TAATTCCCAA 
GGATTCACAC CTCCAGGAAT GGACAGATCC TCTCCTGACA ACAGCCCAGT GCACGGGATG 
TTACGGCAGC CATCCATCAC GACTGGGGTC AATATCCCCA TCATCACAGA ACTCGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAACAGAA AAGAAATGGT TTACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008848111.1
CDS	156..3785
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLKAAARSCYHKDPFVHKNATPKSRLVTEPVEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_008849889.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008849889.2

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTC 
AAGGCTGCAG CTAGATCATG CTATCACAAA GACCCATTTG TGCACAAGAA CGCAACTCCC 
AAATCTCGGC TTGTGACTGA GCCAGTTGAA GATGAACAGC CGTCAACGCT GTCACCCAAA 
AAAAAACAGC GCAACGGGGG CATGAGGAAC TCGCCCAACT CCTCCCCGAA GCTGATGAGG 
CATGACCCCT TACTAATTCC TGGCAATGAT CAGATCGACA ACATGGACTC CAATGTGAAG 
AAATACGACT CTACAGGGAT GTTTCACTGG TGCGCAGCCA AGGAGATCGA GAAAGTCATC 
CTGACTCGAA GCGAAGCTGC CATGACTGTC CTGAGTGGCC ACGTCGTAGT CTGCATCTTT 
GGGGATGTCA GCTCAGCCCT GATTGGCCTC CGGAACCTGG TGATGCCGCT TCGTGCCAGC 
AACTTCCACT ACCATGAGCT CAAACACATT GTGTTTGTGG GATCCATTGA GTACCTCAAG 
CGGGAATGGG AGACACTTCA CAACTTCCCC AAAGTGTCCA TATTGCCGGG TACGCCATTA 
AGTCGGGCTG ATTTAAGGGC TGTCAACATC AACCTCTGTG ACATGTGCGT TATCCTGTCA 
GCCAATCAGA ATAATATTGA TGATACTTCG CTGCAGGACA AGGAATGCAT CTTGGCGTCA 
CTCAACATCA AATCTATGCA GTTTGATGAC AGCATCGGGG TCTTGCAGGC TAATTCCCAA 
GGATTCACAC CTCCAGGAAT GGACAGATCC TCTCCTGACA ACAGCCCAGT GCACGGGATG 
TTACGGCAGC CATCCATCAC GACTGGGGTC AATATCCCCA TCATCACAGA ACTCGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAACAGAA AAGAAATGGT TTACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnma1 ( XM_017802383.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017657872.1 Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X32, mRNA.

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CloneID	ONa32305
Clone ID Related Accession (Same CDS sequence)	XM_017802383.1
Accession Version	XM_017802383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	calcium-activated potassium channel subunit alpha-1 isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008352659.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTC 
AAGGCTGCAG CTAGATCATG CTATCACAAA GACCCATTTG TGCACAAGAA CGCAACTCCC 
AAATCTCGGC TTGTGACTGA GCCAGTTGAA GATGAACAGC CGTCAACGCT GTCACCCAAA 
AAAAAACAGC GCAACGGGGG CATGAGGAAC TCGCCCAACT CCTCCCCGAA GCTGATGAGG 
CATGACCCCT TACTAATTCC TGGCAATGAT CAGATCGACA ACATGGACTC CAATGTGAAG 
AAATACGACT CTACAGGGAT GTTTCACTGG TGCGCAGCCA AGGAGATCGA GAAAGTCATC 
CTGACTCGAA GCGAAGCTGC CATGACTGTC CTGAGTGGCC ACGTCGTAGT CTGCATCTTT 
GGGGATGTCA GCTCAGCCCT GATTGGCCTC CGGAACCTGG TGATGCCGCT TCGTGCCAGC 
AACTTCCACT ACCATGAGCT CAAACACATT GTGTTTGTGG GATCCATTGA GTACCTCAAG 
CGGGAATGGG AGACACTTCA CAACTTCCCC AAAGTGTCCA TATTGCCGGG TACGCCATTA 
AGTCGGGCTG ATTTAAGGGC TGTCAACATC AACCTCTGTG ACATGTGCGT TATCCTGTCA 
GCCAATCAGA ATAATATTGA TGATACTTCG CTGCAGGACA AGGAATGCAT CTTGGCGTCA 
CTCAACATCA AATCTATGCA GTTTGATGAC AGCATCGGGG TCTTGCAGGC TAATTCCCAA 
GGATTCACAC CTCCAGGAAT GGACAGATCC TCTCCTGACA ACAGCCCAGT GCACGGGATG 
TTACGGCAGC CATCCATCAC GACTGGGGTC AATATCCCCA TCATCACAGA ACTCGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAAAAAAG AAATGGTTTA CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017657872.1
CDS	156..3782
Translation	MANGGGGGGGSSGGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYRDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLKAAARSCYHKDPFVHKNATPKSRLVTEPVEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAAKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR

Target ORF information:

RefSeq Version	XM_017802383.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili potassium calcium-activated channel subfamily M alpha 1 (Kcnma1), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017802383.1

ORF Insert Sequence:

ATGGCAAACG GTGGCGGCGG CGGCGGCGGC AGCAGCGGCG GCGGCGGCGG CGGCGGCGGC 
GGAGGCAGCG GTCTTAGAAT GAGTAGCAAT ATCCACGCGA ACCATCTCAG CCTAGACGCG 
TCCTCCTCCT CCTCTTCCTC CTCCTCTTCT TCTTCTTCCT CCTCCTCCTC TTCCTCCTCG 
TCCTCGGTCC ACGAGCCCAA GATGGATGCG CTCATCATCC CGGTGACCAT GGAGGTGCCG 
TGCGACAGCC GGGGCCAACG CATGTGGTGG GCTTTCCTGG CCTCCTCTAT GGTGACTTTC 
TTCGGGGGCC TCTTTATCAT CTTGCTGTGG CGGACGCTCA AGTACCTGTG GACCGTTTGC 
TGCCACTGCG GGGGCAAGAC CAAGGAGGCC CAGAAGATAA ACAATGGCTC CAGCCAGGCA 
GATGGCACTC TCAAGCCGGT GGATGAAAAA GAGGAGGTGG TGGCAGCTGA GGTTGGCTGG 
ATGACCTCCG TGAAGGACTG GGCGGGCGTG ATGATATCCG CCCAGACACT GACTGGCAGA 
GTCCTGGTTG TCTTAGTCTT TGCTCTCAGC ATTGGTGCAC TTGTAATATA CTTCATAGAC 
TCGTCAAACC CGATAGAATC CTGCCAGAAT TTCTACAGAG ATTTCACATT ACAGATCGAC 
ATGGCTTTCA ACGTATTCTT CCTTCTCTAC TTCGGCTTGC GGTTTATTGC AGCCAATGAT 
AAGCTGTGGT TCTGGCTGGA AGTGAACTCT GTGGTAGATT TCTTCACGGT GCCCCCCGTG 
TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA GATTTTTAAG AGCACTCAGA 
CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATAC TTAAAACAAG TAATTCTATC 
AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC TGACTGCGGC TGGGTTCATC 
CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC AAAACAACCA AGCTCTTACA 
TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCCA CTGTTGGTTA TGGGGATGTT 
TATGCAAAAA CCACCCTTGG ACGCCTCTTC ATGGTCTTCT TCATCCTCGG GGGACTGGCC 
ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG GAAACCGCAA GAAATACGGG 
GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG TCTGTGGACA CATTACTCTG 
GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG ACCGGGATGA TGTCAATGTG 
GAGATTGTCT TTCTTCACAA CATTTCCCCT AACCTTGAAC TTGAGGCTCT CTTCAAACGA 
CACTTTACTC AGGTGGAATT TTATCAGGGT TCAGTCCTCA ATCCACATGA TCTGGCCAGA 
GTCAAGATAG AGTCAGCAGA TGCTTGCCTG ATCCTTGCCA ATAAGTACTG CGCTGACCCG 
GATGCAGAAG ATGCTTCCAA CATCATGAGA GTGATCTCCA TAAAGAACTA CCATCCGAAG 
ATCAGAATCA TCACTCAGAT GCTGCAGTAT CACAATAAGG CCCATCTGCT CAACATCCCA 
AGCTGGAATT GGAAAGAGGG TGATGATGCA ATATGCCTCG CAGAGTTGAA GTTGGGTTTC 
ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACAATGC TAGCCAACCT CTTCTCTATG 
AGGTCATTCA TAAAGATTGA GGAAGACACG TGGCAGAAAT ACTACTTGGA AGGAGTCTCC 
AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTTGTGG GTCTGTCCTT CCCTACTGTT 
TGTGAGCTAT GTTTTGTGAA ACTCAAGCTC CTAATGATAG CCATTGAGTA CAAGTCTGCC 
AACCGAGAGA GCCGTATATT AATTAACCCT GGGAACCATC TTAAGATCCA AGAAGGCACT 
TTAGGATTTT TCATCGCGAG TGATGCCAAG GAAGTTAAAA GGGCATTTTT TTACTGCAAG 
GCCTGTCATG ATGACGTCAC AGACCCCAAA AGAATTAAAA AATGCGGCTG CAGACGGCTC 
AAGGCTGCAG CTAGATCATG CTATCACAAA GACCCATTTG TGCACAAGAA CGCAACTCCC 
AAATCTCGGC TTGTGACTGA GCCAGTTGAA GATGAACAGC CGTCAACGCT GTCACCCAAA 
AAAAAACAGC GCAACGGGGG CATGAGGAAC TCGCCCAACT CCTCCCCGAA GCTGATGAGG 
CATGACCCCT TACTAATTCC TGGCAATGAT CAGATCGACA ACATGGACTC CAATGTGAAG 
AAATACGACT CTACAGGGAT GTTTCACTGG TGCGCAGCCA AGGAGATCGA GAAAGTCATC 
CTGACTCGAA GCGAAGCTGC CATGACTGTC CTGAGTGGCC ACGTCGTAGT CTGCATCTTT 
GGGGATGTCA GCTCAGCCCT GATTGGCCTC CGGAACCTGG TGATGCCGCT TCGTGCCAGC 
AACTTCCACT ACCATGAGCT CAAACACATT GTGTTTGTGG GATCCATTGA GTACCTCAAG 
CGGGAATGGG AGACACTTCA CAACTTCCCC AAAGTGTCCA TATTGCCGGG TACGCCATTA 
AGTCGGGCTG ATTTAAGGGC TGTCAACATC AACCTCTGTG ACATGTGCGT TATCCTGTCA 
GCCAATCAGA ATAATATTGA TGATACTTCG CTGCAGGACA AGGAATGCAT CTTGGCGTCA 
CTCAACATCA AATCTATGCA GTTTGATGAC AGCATCGGGG TCTTGCAGGC TAATTCCCAA 
GGATTCACAC CTCCAGGAAT GGACAGATCC TCTCCTGACA ACAGCCCAGT GCACGGGATG 
TTACGGCAGC CATCCATCAC GACTGGGGTC AATATCCCCA TCATCACAGA ACTCGTGAAT 
GACACTAATG TTCAGTTTTT GGACCAAGAT GATGACGATG ACCCCGACAC AGAGCTGTAC 
CTCACGCAAC CCTTTGCGTG TGGGACAGCT TTTGCCGTCA GCGTCCTGGA CTCGCTCATG 
AGTGCGACAT ACTTCAATGA CAATATCCTC ACCCTGATAC GGACCTTGGT GACAGGAGGA 
GCCACACCAG AGCTCGAGGC TCTGATTGCT GAGGAGAATG CACTTCGAGG GGGCTACAGC 
ACTCCCCAGA CACTGGCCAA CAGGGACCGT TGCAGAGTGG CCCAGTTAGC TCTGTTGGAT 
GGGCCCTTTG CAGACTTAGG GGATGGTGGT TGTTATGGTG ATTTGTTCTG CAAAGCTCTG 
AAAACATATA ATATGCTTTG TTTTGGAATT TACCGGCTGA GAGATGCCCA CCTCAGCACC 
CCCAGCCAGT GTACGAAAAG GTACGTCATC ACCAACCCCC CCTACGAGTT TGAGCTGGTG 
CCGACAGACC TGATCTTCTG CCTGATGCAG TTTGACCACA ACGCCGGCCA GTCCCGGGCC 
AGCCTGTCCC ATTCCTCCCA CTCATCACAG TCCTCCAGCA AGAAGAGCTC CTCTGTCCAC 
TCCATCCCGT CCACAGCAAA CCGGCCAAAC CGGCCCAAGT CCAGGGAGTC CCGCGACAAA 
CAGAAAAAAG AAATGGTTTA CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

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オンライン注文

Kcnma1 cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene

Clones

gRNAs

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