Myo1e cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myo1e
Entrez Gene ID	103737155
Full Name	myosin IE
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008836873.3	XP_008835095.1	unconventional myosin-Ie
XM_008836873.2	XP_008835095.1	unconventional myosin-Ie

Pathways from BioSystems
Gene Ontology

The following Myo1e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myo1e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa20241	XM_008836873.3 Latest version!	Nannospalax galili myosin IE (Myo1e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
ONa20241	XM_008836873.2	Nannospalax galili myosin IE (Myo1e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myo1e ( XM_008836873.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008835095.1 Nannospalax galili myosin IE (Myo1e), mRNA.

Price & Availability↑

CloneID	ONa20241
Clone ID Related Accession (Same CDS sequence)	XM_008836873.3 , XM_008836873.2
Accession Version	XM_008836873.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	unconventional myosin-Ie
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008341422.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008836873.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCA AAGGAGCCTA CCGGTACCAC TGGCAGAGTC ACAATGTCAA GCACAGTGGC 
GTGGATGACA TGGTGCTGCT CTCGAAGATC ACAGAGAGCT CAATCGTGGA GAACCTCAAG 
AAGAGATACA TGGACGACTA TATCTTTACA TATATAGGAT CCGTGTTAAT CTCCGTCAAC 
CCTTTCAAGC ATATGCCGTA TTTTGGGGAA AAAGAAATTG AAATGTACCA AGGAGCGGCA 
CAGTATGAAA ATCCACCTCA TATTTATGCA CTCGCAGACA GTATGTACCG AAACATGATC 
ATCGACAGGG AGAACCAATG TGTTATCATC AGCGGTGAGA GCGGTGCTGG TAAGACAGTG 
GCTGCAAAAT ACATCATGGG CTACATCTCC AGAGTGTCCG GAGGAGGCCC CAAGGTCCAG 
CACGTCAAGG ATATCATCCT GCAATCTAAC CCACTGCTGG AGGCCTTCGG GAACGCCAAG 
ACTGTACGCA ACAACAACTC CAGCCGATTT GGAAAATACT TTGAAATCCA GTTCAGTCCA 
GGTGGGGAGC CAGATGGTGG AAAGATCTCC AACTTCCTTC TGGAAAAGTC CAGGGTGGTG 
ATGAGGAACC CTGGAGAGAG AAGCTTTCAC ATCTTCTACC AGCTCATTGA GGGGGCCTCT 
CCAGAGCAGA AGCAAAGCCT GGGCATCACC AGCATGGATT ATTATTACTA CCTGAGCCTC 
TCGGGGTCCT ACAAGGTTGA TGACATTGAT GACAAGCGGG AGTTCCAGGA AACCCTGCAC 
GCCATGAATG TGATTGGGAT CTTCGCAGAA GAACAGACAC TAGTGTTGCA GATTGTGGCA 
GGAATTCTCC ATGTGGGAAA CATCAGCTTC AAAGAAGTGG GCAACTATGC GGCGGTGGAG 
AGTGAGGAGT TTTTGGCTTT TCCTGCATAT CTGCTGGGCA TAAACCAAGA CCGGTTAAAA 
GAGAAGCTAA CGAGCCGGCA GATGGACAGC AAGTGGGGGG GTAAATCAGA ATCCATCCAC 
GTGACCCTCA ATGCAGAGCA GGCTTGCTAC AGCAGGGATG CACTTGCCAA GGCCCTGCAC 
GCCCGCCTCT TTGACTTCTT GGTGGATTCC ATCAACAAAG CCATGGAGAA GGACCATGAG 
GAATACAACA TTGGAGTCCT GGACATCTAT GGCTTTGAAA TATTCCAGAA AAATGGCTTT 
GAACAGTTCT GCATCAATTT TGTTAATGAA AAATTACAGC AGATTTTCAT TGAGCTGACA 
TTAAAAGCAG AACAGGAGGA ATACGTTCAA GAGGGAATAA GATGGACTCC CATTGAGTAC 
TTCAATAACA AAATCGTGTG TGACCTCATA GAGAACAAAG TGAACCCTCC GGGGATCATG 
AGCATCCTGG ATGATGTGTG CGCCACCATG CATGCCGTGG GGGAGGGTGC TGACCAGACA 
CTGCTGCAGA AGCTCCAGAT GCAGATTGGG AGCCACGAGC ACTTCAACAG CTGGAACCAA 
GGCTTCATCA TTCATCACTA TGCCGGGAAG GTTTCCTATG ACATGGATGG CTTTTGCGAG 
AGGAATCGGG ATGTACTGTT CACGGATCTG ATTGAACTTA TGCAAAGCAG TGAGCTACCT 
TTTATAAAGT CTTTATTTCC TGAAAATCTG CAAGCTGACA AGAAAGGGCG TCCAACTACT 
GCAGGAAGCA AAATAAAGAA ACAAGCCAAT GACCTTGTGA GCACGCTGAT GAAATGTACA 
CCCCACTACA TCCGCTGCAT AAAACCCAAT GAAACCAAAA AGCCCAAAGA CTGGGAGGAG 
AGCAGGGTAA AGCATCAAGT TGAATACCTG GGTCTGAAAG AAAACATTCG AGTGAGAAGA 
GCTGGCTATG CTTATAGGCG TGTCTTCCAG AAATTTCTAC AGAGGTACGC CATTCTGACC 
AAAGCCACAT GGCCTGTGTG GAGAGGTGAG GAGAAGCAAG GTGTCCTCCA TCTGCTGCAG 
TCTGTCAACA TGGACACTGA CCAGTTCCAG CTTGGGAAGA GCAAAGTGTT CATCAAAGCC 
CCAGAGTCTC TGTTTCTTCT AGAAGAGATG AGAGAAAGAA AGTACGATGG GTATGCCCGA 
GTGATACAGA AAACATGGAG AAAGTTCGTG GCCCGGAAGA AATATGTCCA AATGAGAGAA 
GAAGCCTCAG ACCTGCTGCT GAACAAAAAG GAGAGGAGAA GAAATAGCAT CAACCGGAAC 
TTCATCGGGG ACTACATCGG GATGGAGGAA CATCCGGAGC TGCAGCAGTT TGTGGGCAAG 
AGGGAGAAGA TTGACTTTGC AGACACGGTC ACCAAGTACG ACAGGCGGTT CAAGGGTGTC 
AAGCGAGATC TGCTGCTAAC TCCAAAGTGC ATGTACTTAA TTGGAAGAGA AAAAATCAAG 
CAAGGCCCAG ACAAAGGCCT GGTGAAGGAA GTCCTGAAGC GGAGAATTGA GATGGAGAGG 
ATCCTGTCTG TGTCCCTCAG CACCATGCAG GATGACATCT TCATTCTCCA TGAGCAAGAG 
TATGACAGTT TGCTTGAATC TGTCTTCAAA ACAGAATTCT TAAGCCTCTT AGCAAAGCGT 
TATGAGGAAA AAACTCAGAA GCAACTACCT CTGAAATTCA GCAATACCCT TGAACTGAAG 
TTAAAAAAGG AGAACTGGGG TCCCTGGAGT GCAGGGGGCT CCCGGCAGGT GCAGTTCCAC 
CAAGGCATTG GCGACCTGGC TATCCTCAAG CCCAGTAACA AAGTGCTGCA GGTCAGCATT 
GGACCCGGGC TGCCCAAGAA CTCCCGTCCT ACCAGAAGGA ATACCGTCCA AAGCAGAGGG 
TATTCTGGCA GAACTCAGAA TGCCAATTAC CCAATGAGAG CCGCCCCTGC ACCACCAGGA 
TATCATCAGA ATGGAGTCAT CAGAAACCAG TTTGTGCCAC CTCCCCACAC TCCTGGAAAC 
CAGAGGTCTA ATCAGAAAAG CCTGTATACC TCCATGGCTC GCCCACCCTT GCCACGGCAA 
CAGTCCACAG GCTCAGACCG ATTGTCACAG ACGCCAGAGA GCCTGGATTT CCTCAAGGTC 
CCGGACCAGG GTGCTGCAGG GGTCCGGAGA CAAACAACCA GCAGGCCTCC TCCAGCAGGC 
GGCAGACCAA AGCCCCAGCC CAAGCCCAAG CCTCAGGTGC CACAGTGCAA AGCTTTATAT 
GCTTACGACG CTCAGGACAC AGATGAGCTC AGCTTCAATG CCAACGATGT CATTGATATT 
ATTAAAGAAG ATCCTTCTGG CTGGTGGACG GGTCGACTAC GAGGCAAGCA GGGCCTGTTT 
CCAAACAACT ATGTGACCAA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008835095.1
CDS	380..3706
Translation	MGSKGAYRYHWQSHNVKHSGVDDMVLLSKITESSIVENLKKRYMDDYIFTYIGSVLISVNPFKHMPYFGEKEIEMYQGAAQYENPPHIYALADSMYRNMIIDRENQCVIISGESGAGKTVAAKYIMGYISRVSGGGPKVQHVKDIILQSNPLLEAFGNAKTVRNNNSSRFGKYFEIQFSPGGEPDGGKISNFLLEKSRVVMRNPGERSFHIFYQLIEGASPEQKQSLGITSMDYYYYLSLSGSYKVDDIDDKREFQETLHAMNVIGIFAEEQTLVLQIVAGILHVGNISFKEVGNYAAVESEEFLAFPAYLLGINQDRLKEKLTSRQMDSKWGGKSESIHVTLNAEQACYSRDALAKALHARLFDFLVDSINKAMEKDHEEYNIGVLDIYGFEIFQKNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVQEGIRWTPIEYFNNKIVCDLIENKVNPPGIMSILDDVCATMHAVGEGADQTLLQKLQMQIGSHEHFNSWNQGFIIHHYAGKVSYDMDGFCERNRDVLFTDLIELMQSSELPFIKSLFPENLQADKKGRPTTAGSKIKKQANDLVSTLMKCTPHYIRCIKPNETKKPKDWEESRVKHQVEYLGLKENIRVRRAGYAYRRVFQKFLQRYAILTKATWPVWRGEEKQGVLHLLQSVNMDTDQFQLGKSKVFIKAPESLFLLEEMRERKYDGYARVIQKTWRKFVARKKYVQMREEASDLLLNKKERRRNSINRNFIGDYIGMEEHPELQQFVGKREKIDFADTVTKYDRRFKGVKRDLLLTPKCMYLIGREKIKQGPDKGLVKEVLKRRIEMERILSVSLSTMQDDIFILHEQEYDSLLESVFKTEFLSLLAKRYEEKTQKQLPLKFSNTLELKLKKENWGPWSAGGSRQVQFHQGIGDLAILKPSNKVLQVSIGPGLPKNSRPTRRNTVQSRGYSGRTQNANYPMRAAPAPPGYHQNGVIRNQFVPPPHTPGNQRSNQKSLYTSMARPPLPRQQSTGSDRLSQTPESLDFLKVPDQGAAGVRRQTTSRPPPAGGRPKPQPKPKPQVPQCKALYAYDAQDTDELSFNANDVIDIIKEDPSGWWTGRLRGKQGLFPNNYVTKI

Target ORF information:

RefSeq Version	XM_008836873.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili myosin IE (Myo1e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008836873.3

ORF Insert Sequence:

ATGGGAAGCA AAGGAGCCTA CCGGTACCAC TGGCAGAGTC ACAATGTCAA GCACAGTGGC 
GTGGATGACA TGGTGCTGCT CTCGAAGATC ACAGAGAGCT CAATCGTGGA GAACCTCAAG 
AAGAGATACA TGGACGACTA TATCTTTACA TATATAGGAT CCGTGTTAAT CTCCGTCAAC 
CCTTTCAAGC ATATGCCGTA TTTTGGGGAA AAAGAAATTG AAATGTACCA AGGAGCGGCA 
CAGTATGAAA ATCCACCTCA TATTTATGCA CTCGCAGACA GTATGTACCG AAACATGATC 
ATCGACAGGG AGAACCAATG TGTTATCATC AGCGGTGAGA GCGGTGCTGG TAAGACAGTG 
GCTGCAAAAT ACATCATGGG CTACATCTCC AGAGTGTCCG GAGGAGGCCC CAAGGTCCAG 
CACGTCAAGG ATATCATCCT GCAATCTAAC CCACTGCTGG AGGCCTTCGG GAACGCCAAG 
ACTGTACGCA ACAACAACTC CAGCCGATTT GGAAAATACT TTGAAATCCA GTTCAGTCCA 
GGTGGGGAGC CAGATGGTGG AAAGATCTCC AACTTCCTTC TGGAAAAGTC CAGGGTGGTG 
ATGAGGAACC CTGGAGAGAG AAGCTTTCAC ATCTTCTACC AGCTCATTGA GGGGGCCTCT 
CCAGAGCAGA AGCAAAGCCT GGGCATCACC AGCATGGATT ATTATTACTA CCTGAGCCTC 
TCGGGGTCCT ACAAGGTTGA TGACATTGAT GACAAGCGGG AGTTCCAGGA AACCCTGCAC 
GCCATGAATG TGATTGGGAT CTTCGCAGAA GAACAGACAC TAGTGTTGCA GATTGTGGCA 
GGAATTCTCC ATGTGGGAAA CATCAGCTTC AAAGAAGTGG GCAACTATGC GGCGGTGGAG 
AGTGAGGAGT TTTTGGCTTT TCCTGCATAT CTGCTGGGCA TAAACCAAGA CCGGTTAAAA 
GAGAAGCTAA CGAGCCGGCA GATGGACAGC AAGTGGGGGG GTAAATCAGA ATCCATCCAC 
GTGACCCTCA ATGCAGAGCA GGCTTGCTAC AGCAGGGATG CACTTGCCAA GGCCCTGCAC 
GCCCGCCTCT TTGACTTCTT GGTGGATTCC ATCAACAAAG CCATGGAGAA GGACCATGAG 
GAATACAACA TTGGAGTCCT GGACATCTAT GGCTTTGAAA TATTCCAGAA AAATGGCTTT 
GAACAGTTCT GCATCAATTT TGTTAATGAA AAATTACAGC AGATTTTCAT TGAGCTGACA 
TTAAAAGCAG AACAGGAGGA ATACGTTCAA GAGGGAATAA GATGGACTCC CATTGAGTAC 
TTCAATAACA AAATCGTGTG TGACCTCATA GAGAACAAAG TGAACCCTCC GGGGATCATG 
AGCATCCTGG ATGATGTGTG CGCCACCATG CATGCCGTGG GGGAGGGTGC TGACCAGACA 
CTGCTGCAGA AGCTCCAGAT GCAGATTGGG AGCCACGAGC ACTTCAACAG CTGGAACCAA 
GGCTTCATCA TTCATCACTA TGCCGGGAAG GTTTCCTATG ACATGGATGG CTTTTGCGAG 
AGGAATCGGG ATGTACTGTT CACGGATCTG ATTGAACTTA TGCAAAGCAG TGAGCTACCT 
TTTATAAAGT CTTTATTTCC TGAAAATCTG CAAGCTGACA AGAAAGGGCG TCCAACTACT 
GCAGGAAGCA AAATAAAGAA ACAAGCCAAT GACCTTGTGA GCACGCTGAT GAAATGTACA 
CCCCACTACA TCCGCTGCAT AAAACCCAAT GAAACCAAAA AGCCCAAAGA CTGGGAGGAG 
AGCAGGGTAA AGCATCAAGT TGAATACCTG GGTCTGAAAG AAAACATTCG AGTGAGAAGA 
GCTGGCTATG CTTATAGGCG TGTCTTCCAG AAATTTCTAC AGAGGTACGC CATTCTGACC 
AAAGCCACAT GGCCTGTGTG GAGAGGTGAG GAGAAGCAAG GTGTCCTCCA TCTGCTGCAG 
TCTGTCAACA TGGACACTGA CCAGTTCCAG CTTGGGAAGA GCAAAGTGTT CATCAAAGCC 
CCAGAGTCTC TGTTTCTTCT AGAAGAGATG AGAGAAAGAA AGTACGATGG GTATGCCCGA 
GTGATACAGA AAACATGGAG AAAGTTCGTG GCCCGGAAGA AATATGTCCA AATGAGAGAA 
GAAGCCTCAG ACCTGCTGCT GAACAAAAAG GAGAGGAGAA GAAATAGCAT CAACCGGAAC 
TTCATCGGGG ACTACATCGG GATGGAGGAA CATCCGGAGC TGCAGCAGTT TGTGGGCAAG 
AGGGAGAAGA TTGACTTTGC AGACACGGTC ACCAAGTACG ACAGGCGGTT CAAGGGTGTC 
AAGCGAGATC TGCTGCTAAC TCCAAAGTGC ATGTACTTAA TTGGAAGAGA AAAAATCAAG 
CAAGGCCCAG ACAAAGGCCT GGTGAAGGAA GTCCTGAAGC GGAGAATTGA GATGGAGAGG 
ATCCTGTCTG TGTCCCTCAG CACCATGCAG GATGACATCT TCATTCTCCA TGAGCAAGAG 
TATGACAGTT TGCTTGAATC TGTCTTCAAA ACAGAATTCT TAAGCCTCTT AGCAAAGCGT 
TATGAGGAAA AAACTCAGAA GCAACTACCT CTGAAATTCA GCAATACCCT TGAACTGAAG 
TTAAAAAAGG AGAACTGGGG TCCCTGGAGT GCAGGGGGCT CCCGGCAGGT GCAGTTCCAC 
CAAGGCATTG GCGACCTGGC TATCCTCAAG CCCAGTAACA AAGTGCTGCA GGTCAGCATT 
GGACCCGGGC TGCCCAAGAA CTCCCGTCCT ACCAGAAGGA ATACCGTCCA AAGCAGAGGG 
TATTCTGGCA GAACTCAGAA TGCCAATTAC CCAATGAGAG CCGCCCCTGC ACCACCAGGA 
TATCATCAGA ATGGAGTCAT CAGAAACCAG TTTGTGCCAC CTCCCCACAC TCCTGGAAAC 
CAGAGGTCTA ATCAGAAAAG CCTGTATACC TCCATGGCTC GCCCACCCTT GCCACGGCAA 
CAGTCCACAG GCTCAGACCG ATTGTCACAG ACGCCAGAGA GCCTGGATTT CCTCAAGGTC 
CCGGACCAGG GTGCTGCAGG GGTCCGGAGA CAAACAACCA GCAGGCCTCC TCCAGCAGGC 
GGCAGACCAA AGCCCCAGCC CAAGCCCAAG CCTCAGGTGC CACAGTGCAA AGCTTTATAT 
GCTTACGACG CTCAGGACAC AGATGAGCTC AGCTTCAATG CCAACGATGT CATTGATATT 
ATTAAAGAAG ATCCTTCTGG CTGGTGGACG GGTCGACTAC GAGGCAAGCA GGGCCTGTTT 
CCAAACAACT ATGTGACCAA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1e ( XM_008836873.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008835095.1 Nannospalax galili myosin IE (Myo1e), mRNA.

Price & Availability↑

CloneID	ONa20241
Clone ID Related Accession (Same CDS sequence)	XM_008836873.3 , XM_008836873.2
Accession Version	XM_008836873.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	unconventional myosin-Ie
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008341422.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008836873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCA AAGGAGCCTA CCGGTACCAC TGGCAGAGTC ACAATGTCAA GCACAGTGGC 
GTGGATGACA TGGTGCTGCT CTCGAAGATC ACAGAGAGCT CAATCGTGGA GAACCTCAAG 
AAGAGATACA TGGACGACTA TATCTTTACA TATATAGGAT CCGTGTTAAT CTCCGTCAAC 
CCTTTCAAGC ATATGCCGTA TTTTGGGGAA AAAGAAATTG AAATGTACCA AGGAGCGGCA 
CAGTATGAAA ATCCACCTCA TATTTATGCA CTCGCAGACA GTATGTACCG AAACATGATC 
ATCGACAGGG AGAACCAATG TGTTATCATC AGCGGTGAGA GCGGTGCTGG TAAGACAGTG 
GCTGCAAAAT ACATCATGGG CTACATCTCC AGAGTGTCCG GAGGAGGCCC CAAGGTCCAG 
CACGTCAAGG ATATCATCCT GCAATCTAAC CCACTGCTGG AGGCCTTCGG GAACGCCAAG 
ACTGTACGCA ACAACAACTC CAGCCGATTT GGAAAATACT TTGAAATCCA GTTCAGTCCA 
GGTGGGGAGC CAGATGGTGG AAAGATCTCC AACTTCCTTC TGGAAAAGTC CAGGGTGGTG 
ATGAGGAACC CTGGAGAGAG AAGCTTTCAC ATCTTCTACC AGCTCATTGA GGGGGCCTCT 
CCAGAGCAGA AGCAAAGCCT GGGCATCACC AGCATGGATT ATTATTACTA CCTGAGCCTC 
TCGGGGTCCT ACAAGGTTGA TGACATTGAT GACAAGCGGG AGTTCCAGGA AACCCTGCAC 
GCCATGAATG TGATTGGGAT CTTCGCAGAA GAACAGACAC TAGTGTTGCA GATTGTGGCA 
GGAATTCTCC ATGTGGGAAA CATCAGCTTC AAAGAAGTGG GCAACTATGC GGCGGTGGAG 
AGTGAGGAGT TTTTGGCTTT TCCTGCATAT CTGCTGGGCA TAAACCAAGA CCGGTTAAAA 
GAGAAGCTAA CGAGCCGGCA GATGGACAGC AAGTGGGGGG GTAAATCAGA ATCCATCCAC 
GTGACCCTCA ATGCAGAGCA GGCTTGCTAC AGCAGGGATG CACTTGCCAA GGCCCTGCAC 
GCCCGCCTCT TTGACTTCTT GGTGGATTCC ATCAACAAAG CCATGGAGAA GGACCATGAG 
GAATACAACA TTGGAGTCCT GGACATCTAT GGCTTTGAAA TATTCCAGAA AAATGGCTTT 
GAACAGTTCT GCATCAATTT TGTTAATGAA AAATTACAGC AGATTTTCAT TGAGCTGACA 
TTAAAAGCAG AACAGGAGGA ATACGTTCAA GAGGGAATAA GATGGACTCC CATTGAGTAC 
TTCAATAACA AAATCGTGTG TGACCTCATA GAGAACAAAG TGAACCCTCC GGGGATCATG 
AGCATCCTGG ATGATGTGTG CGCCACCATG CATGCCGTGG GGGAGGGTGC TGACCAGACA 
CTGCTGCAGA AGCTCCAGAT GCAGATTGGG AGCCACGAGC ACTTCAACAG CTGGAACCAA 
GGCTTCATCA TTCATCACTA TGCCGGGAAG GTTTCCTATG ACATGGATGG CTTTTGCGAG 
AGGAATCGGG ATGTACTGTT CACGGATCTG ATTGAACTTA TGCAAAGCAG TGAGCTACCT 
TTTATAAAGT CTTTATTTCC TGAAAATCTG CAAGCTGACA AGAAAGGGCG TCCAACTACT 
GCAGGAAGCA AAATAAAGAA ACAAGCCAAT GACCTTGTGA GCACGCTGAT GAAATGTACA 
CCCCACTACA TCCGCTGCAT AAAACCCAAT GAAACCAAAA AGCCCAAAGA CTGGGAGGAG 
AGCAGGGTAA AGCATCAAGT TGAATACCTG GGTCTGAAAG AAAACATTCG AGTGAGAAGA 
GCTGGCTATG CTTATAGGCG TGTCTTCCAG AAATTTCTAC AGAGGTACGC CATTCTGACC 
AAAGCCACAT GGCCTGTGTG GAGAGGTGAG GAGAAGCAAG GTGTCCTCCA TCTGCTGCAG 
TCTGTCAACA TGGACACTGA CCAGTTCCAG CTTGGGAAGA GCAAAGTGTT CATCAAAGCC 
CCAGAGTCTC TGTTTCTTCT AGAAGAGATG AGAGAAAGAA AGTACGATGG GTATGCCCGA 
GTGATACAGA AAACATGGAG AAAGTTCGTG GCCCGGAAGA AATATGTCCA AATGAGAGAA 
GAAGCCTCAG ACCTGCTGCT GAACAAAAAG GAGAGGAGAA GAAATAGCAT CAACCGGAAC 
TTCATCGGGG ACTACATCGG GATGGAGGAA CATCCGGAGC TGCAGCAGTT TGTGGGCAAG 
AGGGAGAAGA TTGACTTTGC AGACACGGTC ACCAAGTACG ACAGGCGGTT CAAGGGTGTC 
AAGCGAGATC TGCTGCTAAC TCCAAAGTGC ATGTACTTAA TTGGAAGAGA AAAAATCAAG 
CAAGGCCCAG ACAAAGGCCT GGTGAAGGAA GTCCTGAAGC GGAGAATTGA GATGGAGAGG 
ATCCTGTCTG TGTCCCTCAG CACCATGCAG GATGACATCT TCATTCTCCA TGAGCAAGAG 
TATGACAGTT TGCTTGAATC TGTCTTCAAA ACAGAATTCT TAAGCCTCTT AGCAAAGCGT 
TATGAGGAAA AAACTCAGAA GCAACTACCT CTGAAATTCA GCAATACCCT TGAACTGAAG 
TTAAAAAAGG AGAACTGGGG TCCCTGGAGT GCAGGGGGCT CCCGGCAGGT GCAGTTCCAC 
CAAGGCATTG GCGACCTGGC TATCCTCAAG CCCAGTAACA AAGTGCTGCA GGTCAGCATT 
GGACCCGGGC TGCCCAAGAA CTCCCGTCCT ACCAGAAGGA ATACCGTCCA AAGCAGAGGG 
TATTCTGGCA GAACTCAGAA TGCCAATTAC CCAATGAGAG CCGCCCCTGC ACCACCAGGA 
TATCATCAGA ATGGAGTCAT CAGAAACCAG TTTGTGCCAC CTCCCCACAC TCCTGGAAAC 
CAGAGGTCTA ATCAGAAAAG CCTGTATACC TCCATGGCTC GCCCACCCTT GCCACGGCAA 
CAGTCCACAG GCTCAGACCG ATTGTCACAG ACGCCAGAGA GCCTGGATTT CCTCAAGGTC 
CCGGACCAGG GTGCTGCAGG GGTCCGGAGA CAAACAACCA GCAGGCCTCC TCCAGCAGGC 
GGCAGACCAA AGCCCCAGCC CAAGCCCAAG CCTCAGGTGC CACAGTGCAA AGCTTTATAT 
GCTTACGACG CTCAGGACAC AGATGAGCTC AGCTTCAATG CCAACGATGT CATTGATATT 
ATTAAAGAAG ATCCTTCTGG CTGGTGGACG GGTCGACTAC GAGGCAAGCA GGGCCTGTTT 
CCAAACAACT ATGTGACCAA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008835095.1
CDS	376..3702
Translation	MGSKGAYRYHWQSHNVKHSGVDDMVLLSKITESSIVENLKKRYMDDYIFTYIGSVLISVNPFKHMPYFGEKEIEMYQGAAQYENPPHIYALADSMYRNMIIDRENQCVIISGESGAGKTVAAKYIMGYISRVSGGGPKVQHVKDIILQSNPLLEAFGNAKTVRNNNSSRFGKYFEIQFSPGGEPDGGKISNFLLEKSRVVMRNPGERSFHIFYQLIEGASPEQKQSLGITSMDYYYYLSLSGSYKVDDIDDKREFQETLHAMNVIGIFAEEQTLVLQIVAGILHVGNISFKEVGNYAAVESEEFLAFPAYLLGINQDRLKEKLTSRQMDSKWGGKSESIHVTLNAEQACYSRDALAKALHARLFDFLVDSINKAMEKDHEEYNIGVLDIYGFEIFQKNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVQEGIRWTPIEYFNNKIVCDLIENKVNPPGIMSILDDVCATMHAVGEGADQTLLQKLQMQIGSHEHFNSWNQGFIIHHYAGKVSYDMDGFCERNRDVLFTDLIELMQSSELPFIKSLFPENLQADKKGRPTTAGSKIKKQANDLVSTLMKCTPHYIRCIKPNETKKPKDWEESRVKHQVEYLGLKENIRVRRAGYAYRRVFQKFLQRYAILTKATWPVWRGEEKQGVLHLLQSVNMDTDQFQLGKSKVFIKAPESLFLLEEMRERKYDGYARVIQKTWRKFVARKKYVQMREEASDLLLNKKERRRNSINRNFIGDYIGMEEHPELQQFVGKREKIDFADTVTKYDRRFKGVKRDLLLTPKCMYLIGREKIKQGPDKGLVKEVLKRRIEMERILSVSLSTMQDDIFILHEQEYDSLLESVFKTEFLSLLAKRYEEKTQKQLPLKFSNTLELKLKKENWGPWSAGGSRQVQFHQGIGDLAILKPSNKVLQVSIGPGLPKNSRPTRRNTVQSRGYSGRTQNANYPMRAAPAPPGYHQNGVIRNQFVPPPHTPGNQRSNQKSLYTSMARPPLPRQQSTGSDRLSQTPESLDFLKVPDQGAAGVRRQTTSRPPPAGGRPKPQPKPKPQVPQCKALYAYDAQDTDELSFNANDVIDIIKEDPSGWWTGRLRGKQGLFPNNYVTKI

Target ORF information:

RefSeq Version	XM_008836873.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili myosin IE (Myo1e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008836873.2

ORF Insert Sequence:

ATGGGAAGCA AAGGAGCCTA CCGGTACCAC TGGCAGAGTC ACAATGTCAA GCACAGTGGC 
GTGGATGACA TGGTGCTGCT CTCGAAGATC ACAGAGAGCT CAATCGTGGA GAACCTCAAG 
AAGAGATACA TGGACGACTA TATCTTTACA TATATAGGAT CCGTGTTAAT CTCCGTCAAC 
CCTTTCAAGC ATATGCCGTA TTTTGGGGAA AAAGAAATTG AAATGTACCA AGGAGCGGCA 
CAGTATGAAA ATCCACCTCA TATTTATGCA CTCGCAGACA GTATGTACCG AAACATGATC 
ATCGACAGGG AGAACCAATG TGTTATCATC AGCGGTGAGA GCGGTGCTGG TAAGACAGTG 
GCTGCAAAAT ACATCATGGG CTACATCTCC AGAGTGTCCG GAGGAGGCCC CAAGGTCCAG 
CACGTCAAGG ATATCATCCT GCAATCTAAC CCACTGCTGG AGGCCTTCGG GAACGCCAAG 
ACTGTACGCA ACAACAACTC CAGCCGATTT GGAAAATACT TTGAAATCCA GTTCAGTCCA 
GGTGGGGAGC CAGATGGTGG AAAGATCTCC AACTTCCTTC TGGAAAAGTC CAGGGTGGTG 
ATGAGGAACC CTGGAGAGAG AAGCTTTCAC ATCTTCTACC AGCTCATTGA GGGGGCCTCT 
CCAGAGCAGA AGCAAAGCCT GGGCATCACC AGCATGGATT ATTATTACTA CCTGAGCCTC 
TCGGGGTCCT ACAAGGTTGA TGACATTGAT GACAAGCGGG AGTTCCAGGA AACCCTGCAC 
GCCATGAATG TGATTGGGAT CTTCGCAGAA GAACAGACAC TAGTGTTGCA GATTGTGGCA 
GGAATTCTCC ATGTGGGAAA CATCAGCTTC AAAGAAGTGG GCAACTATGC GGCGGTGGAG 
AGTGAGGAGT TTTTGGCTTT TCCTGCATAT CTGCTGGGCA TAAACCAAGA CCGGTTAAAA 
GAGAAGCTAA CGAGCCGGCA GATGGACAGC AAGTGGGGGG GTAAATCAGA ATCCATCCAC 
GTGACCCTCA ATGCAGAGCA GGCTTGCTAC AGCAGGGATG CACTTGCCAA GGCCCTGCAC 
GCCCGCCTCT TTGACTTCTT GGTGGATTCC ATCAACAAAG CCATGGAGAA GGACCATGAG 
GAATACAACA TTGGAGTCCT GGACATCTAT GGCTTTGAAA TATTCCAGAA AAATGGCTTT 
GAACAGTTCT GCATCAATTT TGTTAATGAA AAATTACAGC AGATTTTCAT TGAGCTGACA 
TTAAAAGCAG AACAGGAGGA ATACGTTCAA GAGGGAATAA GATGGACTCC CATTGAGTAC 
TTCAATAACA AAATCGTGTG TGACCTCATA GAGAACAAAG TGAACCCTCC GGGGATCATG 
AGCATCCTGG ATGATGTGTG CGCCACCATG CATGCCGTGG GGGAGGGTGC TGACCAGACA 
CTGCTGCAGA AGCTCCAGAT GCAGATTGGG AGCCACGAGC ACTTCAACAG CTGGAACCAA 
GGCTTCATCA TTCATCACTA TGCCGGGAAG GTTTCCTATG ACATGGATGG CTTTTGCGAG 
AGGAATCGGG ATGTACTGTT CACGGATCTG ATTGAACTTA TGCAAAGCAG TGAGCTACCT 
TTTATAAAGT CTTTATTTCC TGAAAATCTG CAAGCTGACA AGAAAGGGCG TCCAACTACT 
GCAGGAAGCA AAATAAAGAA ACAAGCCAAT GACCTTGTGA GCACGCTGAT GAAATGTACA 
CCCCACTACA TCCGCTGCAT AAAACCCAAT GAAACCAAAA AGCCCAAAGA CTGGGAGGAG 
AGCAGGGTAA AGCATCAAGT TGAATACCTG GGTCTGAAAG AAAACATTCG AGTGAGAAGA 
GCTGGCTATG CTTATAGGCG TGTCTTCCAG AAATTTCTAC AGAGGTACGC CATTCTGACC 
AAAGCCACAT GGCCTGTGTG GAGAGGTGAG GAGAAGCAAG GTGTCCTCCA TCTGCTGCAG 
TCTGTCAACA TGGACACTGA CCAGTTCCAG CTTGGGAAGA GCAAAGTGTT CATCAAAGCC 
CCAGAGTCTC TGTTTCTTCT AGAAGAGATG AGAGAAAGAA AGTACGATGG GTATGCCCGA 
GTGATACAGA AAACATGGAG AAAGTTCGTG GCCCGGAAGA AATATGTCCA AATGAGAGAA 
GAAGCCTCAG ACCTGCTGCT GAACAAAAAG GAGAGGAGAA GAAATAGCAT CAACCGGAAC 
TTCATCGGGG ACTACATCGG GATGGAGGAA CATCCGGAGC TGCAGCAGTT TGTGGGCAAG 
AGGGAGAAGA TTGACTTTGC AGACACGGTC ACCAAGTACG ACAGGCGGTT CAAGGGTGTC 
AAGCGAGATC TGCTGCTAAC TCCAAAGTGC ATGTACTTAA TTGGAAGAGA AAAAATCAAG 
CAAGGCCCAG ACAAAGGCCT GGTGAAGGAA GTCCTGAAGC GGAGAATTGA GATGGAGAGG 
ATCCTGTCTG TGTCCCTCAG CACCATGCAG GATGACATCT TCATTCTCCA TGAGCAAGAG 
TATGACAGTT TGCTTGAATC TGTCTTCAAA ACAGAATTCT TAAGCCTCTT AGCAAAGCGT 
TATGAGGAAA AAACTCAGAA GCAACTACCT CTGAAATTCA GCAATACCCT TGAACTGAAG 
TTAAAAAAGG AGAACTGGGG TCCCTGGAGT GCAGGGGGCT CCCGGCAGGT GCAGTTCCAC 
CAAGGCATTG GCGACCTGGC TATCCTCAAG CCCAGTAACA AAGTGCTGCA GGTCAGCATT 
GGACCCGGGC TGCCCAAGAA CTCCCGTCCT ACCAGAAGGA ATACCGTCCA AAGCAGAGGG 
TATTCTGGCA GAACTCAGAA TGCCAATTAC CCAATGAGAG CCGCCCCTGC ACCACCAGGA 
TATCATCAGA ATGGAGTCAT CAGAAACCAG TTTGTGCCAC CTCCCCACAC TCCTGGAAAC 
CAGAGGTCTA ATCAGAAAAG CCTGTATACC TCCATGGCTC GCCCACCCTT GCCACGGCAA 
CAGTCCACAG GCTCAGACCG ATTGTCACAG ACGCCAGAGA GCCTGGATTT CCTCAAGGTC 
CCGGACCAGG GTGCTGCAGG GGTCCGGAGA CAAACAACCA GCAGGCCTCC TCCAGCAGGC 
GGCAGACCAA AGCCCCAGCC CAAGCCCAAG CCTCAGGTGC CACAGTGCAA AGCTTTATAT 
GCTTACGACG CTCAGGACAC AGATGAGCTC AGCTTCAATG CCAACGATGT CATTGATATT 
ATTAAAGAAG ATCCTTCTGG CTGGTGGACG GGTCGACTAC GAGGCAAGCA GGGCCTGTTT 
CCAAACAACT ATGTGACCAA GATCTGA