IARS cDNA ORF clone, Myotis brandtii(Brandt's bat)

Gene
Clones
gRNAs

Summary

Gene Symbol	IARS
Entrez Gene ID	102246712
Full Name	isoleucyl-tRNA synthetase
Gene Type	protein-coding
Organism	Myotis brandtii(Brandt's bat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005881654.2	XP_005881716.1	isoleucine--tRNA ligase, cytoplasmic

Pathways from BioSystems
Gene Ontology

The following IARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMy60290	XM_005881654.2 Latest version!	Myotis brandtii isoleucyl-tRNA synthetase (IARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IARS ( XM_005881654.2 ) cDNA ORF clone, Myotis brandtii(Brandt's bat) -> XP_005881716.1 Myotis brandtii isoleucyl-tRNA synthetase (IARS), mRNA.

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CloneID	OMy60290
Clone ID Related Accession (Same CDS sequence)	XM_005881654.2
Accession Version	XM_005881654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-03
Organism	Myotis brandtii(Brandt's bat)
Product	isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005370673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2015 this sequence version replaced XM_005881654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Myotis brandtii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTACAG 
TTCTGGTCCG AATTTAATTG TTTCCAAGAA TGCCTAAAGC AGTCAAAACA TAGGCCAAAA 
TTTACTTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTAC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATAGA TAAGATACTA 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA ATGGGGATTG TGGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCTA CTGTTACCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACA CTCTATCCAG AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AACAACTTTA TGATAAAGGC CTTGTTTATA GAGGTATGAA AGTCATGCCT 
TTTTCCACTG CATGCAACAC TCCACTTTCT AACTTCGAAT CTCACCAGAA TTACAAGGAC 
GTTCAAGATC CTTCAATATT TGTTACCTTT CCTTTGGAGG AAGACGAAAA TATATCTTTG 
GTTGCTTGGA CAACTACTCC CTGGACTCTG CCTAGTAACC TTGCCCTCTG TGTTAATCCG 
GATATGCAGT ATGTCAAGAT TAAAGATGTT GTCAGAGGAA AATCATTCAT TTTAATGGAA 
GCCAGATTAC CGGCCCTCTA CAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCA 
GGTGCCTCTC TCCAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTTGT GAAGTGGAAA 
AAGCATGGCG CCTTTACAGT GCTTGTCGAC AATTATGTGA GGGAGGAAGA AGGCACTGGG 
GTGGTACATC AAGCTCCTTA CTTTGGTGCT GATGACTATC GGGTCTGTAT GGACTTCAAC 
ATCATTCAGA AAGATTCTGT CCCTGTGTGC CCTGTGGATG CTTCAGGCTG TTTCACAGCG 
GAAGTGACAG ATTTCATGGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAGGATG 
CTGAAGGAAC AAGGCCGACT TCTTGTTGCC AGCACCTTCA CTCATAGCTA CCCTTTCTGC 
TGGAGATCAG ATACCCCCCT CATTTACAAA GCGGTGCCCA GCTGGTTTGT GCGGGTTGAG 
CACATGGTGG ACCAGCTGAT GAGGAACAAT GACCTGTGTT ACTGGGTCCC GGAGTTTGTG 
AGAGAAAAGC GGTTTGGAAA TTGGCTGAAA GATGCACGAG ACTGGGCCAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGAGG ACTTCGAGGA GATAGTATGC 
ATTGGGTCAA TGGCAGAACT TGAAGAACTG TCAGGAACAA AGATCTCAGA TCTCCACAGA 
GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGGA AGGGATTGCT GCGTCGCGTG 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA AGTTCATTAT 
CCATTTGAAA ATAAGAAGGA GTTTGAAGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC 
ATTGATCAAA CCAGAGGATG GTTTTACACT CTGCTGGTGC TGGCCACAGC TCTCTTTGGA 
CAACCACCTT TCAGGAATGT GATTGTGAAT GGGCTCGTCC TTGCAAGTGA TGGCCAGAAA 
ATGAGCAAAC GAAAAAAGAA TTATCCAGAT CCACTTTCCA TCATCCATAA ATATGGTGCT 
GACGCCCTCA GATTGTATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAGGAGGAAG GTGTTCGCGA TGTTCTGAAG GACGTGCTGC TCCCTTGGTA CAATGCCTAT 
CGCTTCTTCA TCCAGAATGT CCTGAGGCTG CAGAAGGAGG AAGAAATGGA ATTCCTCTAC 
AATGAGAACA CGGTTAAAGA AAGTGCCAAC ATTACAGACC AGTGGATCCT GTCCTTCATG 
CAGTCGCTCA TTGCCTTCTT TGAGACCGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCCCGCCTGG TCAAGTTTGT CGATGTTCTG ACCAATTGGT ATGTCAGGAT GAACCGCCGA 
AGATTAAAGG GCGAAAATGG GAAGGAGGAT TGTGTTACGG CCCTGGAAAC CTTGTTCAGT 
GTTCTGCTTT CTCTGTGCAG ACTGATGGCC CCGTATACAC CTTTTCTTAC TGAATTGATG 
TACCAGAATC TTAAGATGCT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACACTCAGC 
ATCCACTACC TCATGCTGCC CCGTGTTCGA GAGGAATTGA TTGACAAGAA AACTGAGAGT 
GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGGCGAG TGATTCGTGA CCGCAAAACT 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTCGTTATCC ATCAAGATCC AGAAGCTCTC 
GGTGATATCA GATCTTTGGA GAAGTATATT ATTGAGGAGT TGAATGTTCA AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGTATT CGCCTAAGGG CAGAGCCAGA TCACATGATC 
CTGGGGAAGC GGCTGAAGGG AGCCTTCAAG GCAGTGATGA CGTCCATCAA GCAGCTGAGC 
AGTGAGGAGC TGGAGCAGTT CCAGAAGCGT GGGTCCATTG TTGTGGAAGG CCATGAATTG 
CATGAAGATG ACATTCGCCT TATGTACACC TTTGATCAAG CAACAGTTGA GACAGCCCAG 
TTTGAAGCAC ACTCGGACGC TCAGGCTTTG GTTCTCTTAG ATGTCACCCC TGACCAGGCA 
ATGGTGGATC AAGGGGTGGC TCGGGAAGTC ATCAATCGCA TCCAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCCACTGA TGAAATAACA GTTTATTACA CAGCAAAGTC TGAAGGAAAT 
TATCTGAGCA CCATTATTGA AAGCCACATG GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCAT ATCCAGTCGC TACATCAGAT AAAATCCTTA TTCAGGAAAA AATGCAGTTA 
AAGGGGTCTG ACCTGGAAAT TACACTCACC AGGGGATCCT CCACACCTGG CCCTGCTTGT 
GCATATGTCA ATCTTCACAT TTGTGCAAAT GGCAGTGAGC AAGGTGGAGT ACTGCTTCTG 
GAAAATCCTA AAGGCGATAA TCGGTTAGAC CTCCTAAAGC TGAAGAGTGT TGTTACCAGC 
ATTTTTGGTG TGAAGAACAC AGAGCTGGCG CTCTTCCACG GGGAAACAGA AATACAAAAC 
CAAACCGACT TACTGAGTCT TAGTGGAAAG ATGCTGGGTG TGACTGCAGG ACCAGCTCCC 
TCTGTGATTG ACAGTCCTGG TACTCTTCTC TGTCGGTATA TCAACCTACA GCTACTGAAT 
GCAGACCCAC AAGAGTGTTT AATGGGAACA GTGGGCACCC TCCTGCTGGA AAACCCACTT 
GGGCAGAATG GACTCACCTA TGAAGGTCTT CTGTGTGAAG CAGCCAAGAT GTTTGGCCTT 
CGCAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA TGGATGAAAT TACAGAAGAA 
ATTCCCCTGA AGACTTTGAA CACGAAGACT GTGTATGTTT CTGTATTTCC AACAACGGCT 
GACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005881716.1
CDS	87..3875
Translation	MVQQVPENINFPAEEEKILQFWSEFNCFQECLKQSKHRPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKILGIRGPEDVAKMGIVEYNNQCRAIVMRYSTEWKSTVTRLGRWIDFDNDYKTLYPEFMESVWWVFKQLYDKGLVYRGMKVMPFSTACNTPLSNFESHQNYKDVQDPSIFVTFPLEEDENISLVAWTTTPWTLPSNLALCVNPDMQYVKIKDVVRGKSFILMEARLPALYKLESDYEILERFPGASLQGKKYRPLFDYFVKWKKHGAFTVLVDNYVREEEGTGVVHQAPYFGADDYRVCMDFNIIQKDSVPVCPVDASGCFTAEVTDFMGQYVKDADKNIIRMLKEQGRLLVASTFTHSYPFCWRSDTPLIYKAVPSWFVRVEHMVDQLMRNNDLCYWVPEFVREKRFGNWLKDARDWAISRNRYWGTPIPLWVSEDFEEIVCIGSMAELEELSGTKISDLHRESIDHLTIPSRCGKGLLRRVSEVFDCWFESGSMPYAQVHYPFENKKEFEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFRNVIVNGLVLASDGQKMSKRKKNYPDPLSIIHKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVLRLQKEEEMEFLYNENTVKESANITDQWILSFMQSLIAFFETEMAAYRLYTVVPRLVKFVDVLTNWYVRMNRRRLKGENGKEDCVTALETLFSVLLSLCRLMAPYTPFLTELMYQNLKMLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALGDIRSLEKYIIEELNVQKVTLSTDKNKYGIRLRAEPDHMILGKRLKGAFKAVMTSIKQLSSEELEQFQKRGSIVVEGHELHEDDIRLMYTFDQATVETAQFEAHSDAQALVLLDVTPDQAMVDQGVAREVINRIQKLRKKCNLVPTDEITVYYTAKSEGNYLSTIIESHMEFIFATIKAPLKPYPVATSDKILIQEKMQLKGSDLEITLTRGSSTPGPACAYVNLHICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELALFHGETEIQNQTDLLSLSGKMLGVTAGPAPSVIDSPGTLLCRYINLQLLNADPQECLMGTVGTLLLENPLGQNGLTYEGLLCEAAKMFGLRSRKLKLFLNETQMDEITEEIPLKTLNTKTVYVSVFPTTADF

Target ORF information:

RefSeq Version	XM_005881654.2
Organism	Myotis brandtii(Brandt's bat)
Definition	Myotis brandtii isoleucyl-tRNA synthetase (IARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005881654.2

ORF Insert Sequence:

ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTACAG 
TTCTGGTCCG AATTTAATTG TTTCCAAGAA TGCCTAAAGC AGTCAAAACA TAGGCCAAAA 
TTTACTTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTAC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATAGA TAAGATACTA 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA ATGGGGATTG TGGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCTA CTGTTACCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACA CTCTATCCAG AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AACAACTTTA TGATAAAGGC CTTGTTTATA GAGGTATGAA AGTCATGCCT 
TTTTCCACTG CATGCAACAC TCCACTTTCT AACTTCGAAT CTCACCAGAA TTACAAGGAC 
GTTCAAGATC CTTCAATATT TGTTACCTTT CCTTTGGAGG AAGACGAAAA TATATCTTTG 
GTTGCTTGGA CAACTACTCC CTGGACTCTG CCTAGTAACC TTGCCCTCTG TGTTAATCCG 
GATATGCAGT ATGTCAAGAT TAAAGATGTT GTCAGAGGAA AATCATTCAT TTTAATGGAA 
GCCAGATTAC CGGCCCTCTA CAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCA 
GGTGCCTCTC TCCAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTTGT GAAGTGGAAA 
AAGCATGGCG CCTTTACAGT GCTTGTCGAC AATTATGTGA GGGAGGAAGA AGGCACTGGG 
GTGGTACATC AAGCTCCTTA CTTTGGTGCT GATGACTATC GGGTCTGTAT GGACTTCAAC 
ATCATTCAGA AAGATTCTGT CCCTGTGTGC CCTGTGGATG CTTCAGGCTG TTTCACAGCG 
GAAGTGACAG ATTTCATGGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAGGATG 
CTGAAGGAAC AAGGCCGACT TCTTGTTGCC AGCACCTTCA CTCATAGCTA CCCTTTCTGC 
TGGAGATCAG ATACCCCCCT CATTTACAAA GCGGTGCCCA GCTGGTTTGT GCGGGTTGAG 
CACATGGTGG ACCAGCTGAT GAGGAACAAT GACCTGTGTT ACTGGGTCCC GGAGTTTGTG 
AGAGAAAAGC GGTTTGGAAA TTGGCTGAAA GATGCACGAG ACTGGGCCAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGAGG ACTTCGAGGA GATAGTATGC 
ATTGGGTCAA TGGCAGAACT TGAAGAACTG TCAGGAACAA AGATCTCAGA TCTCCACAGA 
GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGGA AGGGATTGCT GCGTCGCGTG 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA AGTTCATTAT 
CCATTTGAAA ATAAGAAGGA GTTTGAAGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC 
ATTGATCAAA CCAGAGGATG GTTTTACACT CTGCTGGTGC TGGCCACAGC TCTCTTTGGA 
CAACCACCTT TCAGGAATGT GATTGTGAAT GGGCTCGTCC TTGCAAGTGA TGGCCAGAAA 
ATGAGCAAAC GAAAAAAGAA TTATCCAGAT CCACTTTCCA TCATCCATAA ATATGGTGCT 
GACGCCCTCA GATTGTATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAGGAGGAAG GTGTTCGCGA TGTTCTGAAG GACGTGCTGC TCCCTTGGTA CAATGCCTAT 
CGCTTCTTCA TCCAGAATGT CCTGAGGCTG CAGAAGGAGG AAGAAATGGA ATTCCTCTAC 
AATGAGAACA CGGTTAAAGA AAGTGCCAAC ATTACAGACC AGTGGATCCT GTCCTTCATG 
CAGTCGCTCA TTGCCTTCTT TGAGACCGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCCCGCCTGG TCAAGTTTGT CGATGTTCTG ACCAATTGGT ATGTCAGGAT GAACCGCCGA 
AGATTAAAGG GCGAAAATGG GAAGGAGGAT TGTGTTACGG CCCTGGAAAC CTTGTTCAGT 
GTTCTGCTTT CTCTGTGCAG ACTGATGGCC CCGTATACAC CTTTTCTTAC TGAATTGATG 
TACCAGAATC TTAAGATGCT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACACTCAGC 
ATCCACTACC TCATGCTGCC CCGTGTTCGA GAGGAATTGA TTGACAAGAA AACTGAGAGT 
GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGGCGAG TGATTCGTGA CCGCAAAACT 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTCGTTATCC ATCAAGATCC AGAAGCTCTC 
GGTGATATCA GATCTTTGGA GAAGTATATT ATTGAGGAGT TGAATGTTCA AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGTATT CGCCTAAGGG CAGAGCCAGA TCACATGATC 
CTGGGGAAGC GGCTGAAGGG AGCCTTCAAG GCAGTGATGA CGTCCATCAA GCAGCTGAGC 
AGTGAGGAGC TGGAGCAGTT CCAGAAGCGT GGGTCCATTG TTGTGGAAGG CCATGAATTG 
CATGAAGATG ACATTCGCCT TATGTACACC TTTGATCAAG CAACAGTTGA GACAGCCCAG 
TTTGAAGCAC ACTCGGACGC TCAGGCTTTG GTTCTCTTAG ATGTCACCCC TGACCAGGCA 
ATGGTGGATC AAGGGGTGGC TCGGGAAGTC ATCAATCGCA TCCAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCCACTGA TGAAATAACA GTTTATTACA CAGCAAAGTC TGAAGGAAAT 
TATCTGAGCA CCATTATTGA AAGCCACATG GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCAT ATCCAGTCGC TACATCAGAT AAAATCCTTA TTCAGGAAAA AATGCAGTTA 
AAGGGGTCTG ACCTGGAAAT TACACTCACC AGGGGATCCT CCACACCTGG CCCTGCTTGT 
GCATATGTCA ATCTTCACAT TTGTGCAAAT GGCAGTGAGC AAGGTGGAGT ACTGCTTCTG 
GAAAATCCTA AAGGCGATAA TCGGTTAGAC CTCCTAAAGC TGAAGAGTGT TGTTACCAGC 
ATTTTTGGTG TGAAGAACAC AGAGCTGGCG CTCTTCCACG GGGAAACAGA AATACAAAAC 
CAAACCGACT TACTGAGTCT TAGTGGAAAG ATGCTGGGTG TGACTGCAGG ACCAGCTCCC 
TCTGTGATTG ACAGTCCTGG TACTCTTCTC TGTCGGTATA TCAACCTACA GCTACTGAAT 
GCAGACCCAC AAGAGTGTTT AATGGGAACA GTGGGCACCC TCCTGCTGGA AAACCCACTT 
GGGCAGAATG GACTCACCTA TGAAGGTCTT CTGTGTGAAG CAGCCAAGAT GTTTGGCCTT 
CGCAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA TGGATGAAAT TACAGAAGAA 
ATTCCCCTGA AGACTTTGAA CACGAAGACT GTGTATGTTT CTGTATTTCC AACAACGGCT 
GACTTCTAA