Rasgrf1 cDNA ORF clone, Mus pahari(shrew mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rasgrf1
Entrez Gene ID	110327751
Full Name	Ras protein specific guanine nucleotide releasing factor 1
Gene Type	protein-coding
Organism	Mus pahari(shrew mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021206939.1	XP_021062598.1	ras-specific guanine nucleotide-releasing factor 1 isoform X2
XM_021206938.2	XP_021062597.1	ras-specific guanine nucleotide-releasing factor 1 isoform X1
XM_021206938.1	XP_021062597.1	ras-specific guanine nucleotide-releasing factor 1 isoform X1
XM_021206941.1	XP_021062600.1	ras-specific guanine nucleotide-releasing factor 1 isoform X4
XM_021206941.2	XP_021062600.1	ras-specific guanine nucleotide-releasing factor 1 isoform X4
XM_021206939.2	XP_021062598.1	ras-specific guanine nucleotide-releasing factor 1 isoform X2
XM_021206940.1	XP_021062599.1	ras-specific guanine nucleotide-releasing factor 1 isoform X3
XM_029543434.1	XP_029399294.1	ras-specific guanine nucleotide-releasing factor 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following Rasgrf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasgrf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMv47902	XM_021206941.2 Latest version!	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OMv78225	XM_029543434.1 Latest version!	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMv47899	XM_021206938.2 Latest version!	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMv47900	XM_021206939.2 Latest version!	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMv47901	XM_021206940.1 Latest version!	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OMv47899	XM_021206938.1	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMv47902	XM_021206941.1	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OMv47900	XM_021206939.1	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rasgrf1 ( XM_021206941.1 ) cDNA ORF clone, Mus pahari(shrew mouse) -> XP_021062600.1 Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMv47902
Clone ID Related Accession (Same CDS sequence)	XM_021206941.1 , XM_021206941.2
Accession Version	XM_021206941.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-18
Organism	Mus pahari(shrew mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034599.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus pahari Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCGTC ATAGCAGGAG TCGACGTCGA 
CGTAATGTTC TGAGAAGACA ACCAACCAGG ATCTCAAAAA CATTCAGGCT GGTGGCAATT 
GAATTGACTG GGATGACAGC CACCCTTGAA GTCTCCTTCT CCATAACTAG GACAGAGATC 
TGTACTGTTG CTCCAGCTCC ACAGACATGG ATGGGAAACC CAGAAGACCT GACAACGCTG 
CGTGACAGAG TGGAGTCGAA GCGTCTCTTC CACCATCCCT GTAGATTGAT CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021062600.1
CDS	219..755
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQRHSRSRRRRNVLRRQPTRISKTFRLVAIELTGMTATLEVSFSITRTEICTVAPAPQTWMGNPEDLTTLRDRVESKRLFHHPCRLII

Target ORF information:

RefSeq Version	XM_021206941.1
Organism	Mus pahari(shrew mouse)
Definition	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021206941.1

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCGTC ATAGCAGGAG TCGACGTCGA 
CGTAATGTTC TGAGAAGACA ACCAACCAGG ATCTCAAAAA CATTCAGGCT GGTGGCAATT 
GAATTGACTG GGATGACAGC CACCCTTGAA GTCTCCTTCT CCATAACTAG GACAGAGATC 
TGTACTGTTG CTCCAGCTCC ACAGACATGG ATGGGAAACC CAGAAGACCT GACAACGCTG 
CGTGACAGAG TGGAGTCGAA GCGTCTCTTC CACCATCCCT GTAGATTGAT CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf1 ( XM_021206941.2 ) cDNA ORF clone, Mus pahari(shrew mouse) -> XP_021062600.1 Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMv47902
Clone ID Related Accession (Same CDS sequence)	XM_021206941.1 , XM_021206941.2
Accession Version	XM_021206941.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Mus pahari(shrew mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034599.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 18, 2019 this sequence version replaced XM_021206941.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus pahari Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCGTC ATAGCAGGAG TCGACGTCGA 
CGTAATGTTC TGAGAAGACA ACCAACCAGG ATCTCAAAAA CATTCAGGCT GGTGGCAATT 
GAATTGACTG GGATGACAGC CACCCTTGAA GTCTCCTTCT CCATAACTAG GACAGAGATC 
TGTACTGTTG CTCCAGCTCC ACAGACATGG ATGGGAAACC CAGAAGACCT GACAACGCTG 
CGTGACAGAG TGGAGTCGAA GCGTCTCTTC CACCATCCCT GTAGATTGAT CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021062600.1
CDS	413..949
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQRHSRSRRRRNVLRRQPTRISKTFRLVAIELTGMTATLEVSFSITRTEICTVAPAPQTWMGNPEDLTTLRDRVESKRLFHHPCRLII

Target ORF information:

RefSeq Version	XM_021206941.2
Organism	Mus pahari(shrew mouse)
Definition	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021206941.2

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCGTC ATAGCAGGAG TCGACGTCGA 
CGTAATGTTC TGAGAAGACA ACCAACCAGG ATCTCAAAAA CATTCAGGCT GGTGGCAATT 
GAATTGACTG GGATGACAGC CACCCTTGAA GTCTCCTTCT CCATAACTAG GACAGAGATC 
TGTACTGTTG CTCCAGCTCC ACAGACATGG ATGGGAAACC CAGAAGACCT GACAACGCTG 
CGTGACAGAG TGGAGTCGAA GCGTCTCTTC CACCATCCCT GTAGATTGAT CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf1 ( XM_029543434.1 ) cDNA ORF clone, Mus pahari(shrew mouse) -> XP_029399294.1 Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMv78225
Clone ID Related Accession (Same CDS sequence)	XM_029543434.1
Accession Version	XM_029543434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Mus pahari(shrew mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034599.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus pahari Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGGTAC TGTTGCTCCA GCTCCACAGA 
CATGGATGGG AAACCCAGAA GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029399294.1
CDS	403..729
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQVLLLQLHRHGWETQKT

Target ORF information:

RefSeq Version	XM_029543434.1
Organism	Mus pahari(shrew mouse)
Definition	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029543434.1

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGGTAC TGTTGCTCCA GCTCCACAGA 
CATGGATGGG AAACCCAGAA GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf1 ( XM_021206938.2 ) cDNA ORF clone, Mus pahari(shrew mouse) -> XP_021062597.1 Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMv47899
Clone ID Related Accession (Same CDS sequence)	XM_021206938.2 , XM_021206938.1
Accession Version	XM_021206938.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Mus pahari(shrew mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034599.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 18, 2019 this sequence version replaced XM_021206938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus pahari Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ACTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGCTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCCG CCATCGCTCG TGCCAGCTAC AAGATACTGG CTACAGAGCA CGAGGCGCTC 
ATGCAGAAGT ACTTGCACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAACAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGGTAACT 
ATTACAAACC TGATCAAGGA CAATGACCGT GTTCAATCCA GCAACAAGGC CGCTTCTGCT 
GATGATGAGG ACAGTGACAT CAAGAAAATT AAGAAGGTTC AGAGCTTCCT TCGGGGATGG 
CTGTGCCGTC GAAAGTGGAA GAACATCATC CAGGACTACA TCCGGTCTCC CCACGCGGAC 
AGCATGCGCA AGAGGAACCA GGTGGTATTC AGCATGCTGG AGGCGGAGGC TGAGTACGTG 
CAGCAGCTAC ACATCCTTGT CAACAACTTC CTGCGCCCAC TGCGCATGGC CGCCAGCTCT 
AAGAAACCCC CTATAACACA CGACGACGTC AGCAGTATCT TTCTGAACAG CGAGACCATC 
ATGTTCCTGC ATCAGATCTT CTACCAAGGC CTGAAGGCCC GTATCTCTAG CTGGCCCACC 
CTGGTGCTGG CGGACCTGTT CGACATCCTG CTGCCCATGC TGAACATCTA CCAGGAGTTC 
GTCCGCAACC ACCAGTACAG CCTCCAGATC CTTGCTCACT GCAAGCAAAA CCGGGACTTT 
GACAAGCTCC TCAAGCAGTA TGAGGCCAAG CCAGACTGCG AGGAGCGCAC ACTGGAGACC 
TTCCTCACCT ACCCAATGTT CCAGATCCCC AGGTACATCC TGACACTCCA TGAGCTGCTG 
GCCCACACAC CCCATGAGCA TGTGGAGCGT AACAGCCTGG ACTATGCCAA ATCCAAACTA 
GAGGAACTGT CCAGGATCAT GCATGACGAA GTCAGTGAGA CCGAAAACAT CCGCAAAAAC 
CTGGCCATTG AGCGTATGAT CACTGAGGGC TGTGAGATCC TCCTGGACAC CAGCCAGACC 
TTCGTGCGCC AAGGTTCCCT CATGCAGGTG TCCTTGTCAG AAAAGAGCAA GTCCTCTAGG 
GGCCGCCTGG GGTCTCTGTC CATTAAGAAA GAGGGTGAGC GCCAGTGTTT CCTGTTCTCC 
AAGCATCTCA TCATCTGCAC CAGAGGCTCT GGTGGGAAAC TGCACCTAAC CAAGAATGGA 
GTCATATCCC TCATTGACTG CACCTTACTG GATGAACCCG AAAACCTGGA CGACGAAGCC 
AAAGGAGCTG GACCAGAGAT AGAGCACCTG GAGTTTAAGA TCGGGGTGGA GCCAAAGGAC 
TCCCCGCCCT TCACCGTCAT CCTGGTGGCC TCATCCAGGC AGGAGAAGGC AGCATGGACC 
AGTGACATCA TCCAGTGCGT GGATAATATC CGCTGCAATG GACTCATGAT GAATGCCTTC 
GAAGAAAATT CCAAGGTCAC CGTGCCTCAG ATGATCAAGT CTGACGCTTC CTTATATTGT 
GATGATGTTG ACATTCGCTT CAGCAAGACC ATGAATTCTT GCAAAGTGCT TCAGATCCGC 
TATGCCAGCG TGGAGCGCCT GCTGGAGCGC CTGACCGATC TTCGCTTCCT GAGCATTGAC 
TTTCTCAACA CCTTCCTGCA TTCCTATCGA GTCTTCACCA CTGCTATGGT GGTCCTGGAC 
AAGCTAATCA ACATCTACAG AAAACCCATG AGTGCCATTC CTGCCAGGTC CCTGGAACTC 
TTGTTTTCCA GCAGCCACAA CGCCAAGCTT CTGTACGGAG ATGCCCCCAA ATCCCCTCGC 
GCCAGCCGCA AGTTCTCCTC GCCGCCGCCC TTGGCCATCG GGACATCGTC CCCGAGCCGC 
CGTCGGAAGC TGTCCCTCAA CATCCCCATC ATCACAGGTG GCAAGGCACT GGAACTGGCT 
TCACTTGGGT GCAGCTCCGA TAGCTACGCC AACATACACT CACCCATATC CCCCTTCGGC 
AAAACCACAC TGGACACTGG CAAGCTCTGT ATGGCCAGCA GCCTACCCAA GACTCCAGAG 
GAGATTGACG TGCCTACCAC AATTCCAGAA AAGCCTGGGG AGCTCTCAGC CTCCAGAAAG 
CAGAGCTCTG ACATCCTGAA AGGAGAGTCT GATGGCGACC AGAACCACAG TGATGAAGAC 
AACACAGAAG TGTCGCCAGT TAAATCTCCA CCAACACCGA AGTCGTTCTT AAACAGAACC 
ATCACAGAGT TCCCATTCTT TAACTACAAC AACGGAGTCC TGATGACCAC GTGTCGCGAT 
CTGGTGGACA ATAACCGCAG CACTCTCTCA GCTACTTCTG CCTTTGCCAT AGCGACTGCA 
GGAGCCAATG AAGGCCCCTC AAACAAGGAG GTCTTTCGAA GGATGTCTTT GGCCAACACA 
GGCTTTTCCT CTGACCAGAG AAATATCGAC AAGGAGTTTG TGATCCGCAG AGCTGCCACC 
AATCGAGTGT TGAATGTGCT CCGCCACTGG GTCACCAAGC ACACCCAGGA CTTTGATACC 
GACGATACGC TCAAATACAG GGTGATCTGC TTCTTGGAGG AGGTCATGCA TGACCCAGAC 
CTTCTGACCC AAGAGCGAAA GGCAGCAGCC AACATCATCA GGACTCTGAC CCTAGAAGAA 
ACAACTGAAC AACATAGCAT GCTGGAAGAA GTCATACTGA TGACGGAGGG TGTGAAGACT 
GAGCCCTTCG AAAGCCACCC AGCCCTGGAG ATAGCAGAGC AGCTGACCCT ACTGGATCAC 
CTTGTCTTCA AGAGTATTCC TTATGAAGAG TTCTTTGGCC AGGGCTGGAT GAAGATGGAG 
AAGTATGAAA GGACACCTTA CATTATGAAA ACCACCAAAC ACTTCAACCA TGTCAGCAAC 
TTCATTGCTT CAGAAATTAT CCGAAACGAG GACATCAACG CAAGGGCAAG CGCCATCGAG 
AAGTGGGTGG CCGTGGCCGA CATATGTCGC TGCCTGCACA ACTACAATGC TGTGCTGGAG 
ATCACCTCCT CCATCAACCG CAGCGCCATC TTCCGACTCA AAAAGACATG GCTCAAAGTT 
TCTAAGCAGA CGAAATCTTT GCTTGACAAG CTCCAAAAGC TTGTGTCATC AGATGGCAGA 
TTTAAGAACC TCAGAGAGGC TTTGAGAAAT TGCGATCCAC CTTGTGTCCC TTACCTGGGG 
ATGTATCTCA CCGACTTGGT GTTCATCGAG GAGGGGACAC CCAATTACAC AGAGGACGGC 
CTGGTCAACT TCTCCAAGAT GAGAATGATC TCCCATATTA TCCGAGAGAT TCGCCAATTT 
CAGCAGACGA CCTACAAAAT CGAGCCCCAG CCAAAGGTAA TTCAGTACTT ACTGGATGAA 
TCTTTCATGT TGGACGAAGA GAGTCTGTAT GAGTCTTCGC TCCTAATTGA ACCAAAACTC 
CCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021062597.1
CDS	401..4189
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQQHYFTVNFSNDSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKTEVTITNLIKDNDRVQSSNKAASADDEDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLMQVSLSEKSKSSRGRLGSLSIKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLDEPENLDDEAKGAGPEIEHLEFKIGVEPKDSPPFTVILVASSRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAMVVLDKLINIYRKPMSAIPARSLELLFSSSHNAKLLYGDAPKSPRASRKFSSPPPLAIGTSSPSRRRKLSLNIPIITGGKALELASLGCSSDSYANIHSPISPFGKTTLDTGKLCMASSLPKTPEEIDVPTTIPEKPGELSASRKQSSDILKGESDGDQNHSDEDNTEVSPVKSPPTPKSFLNRTITEFPFFNYNNGVLMTTCRDLVDNNRSTLSATSAFAIATAGANEGPSNKEVFRRMSLANTGFSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFLEEVMHDPDLLTQERKAAANIIRTLTLEETTEQHSMLEEVILMTEGVKTEPFESHPALEIAEQLTLLDHLVFKSIPYEEFFGQGWMKMEKYERTPYIMKTTKHFNHVSNFIASEIIRNEDINARASAIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLLDKLQKLVSSDGRFKNLREALRNCDPPCVPYLGMYLTDLVFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIEPQPKVIQYLLDESFMLDEESLYESSLLIEPKLPT

Target ORF information:

RefSeq Version	XM_021206938.2
Organism	Mus pahari(shrew mouse)
Definition	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021206938.2

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ACTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGCTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCCG CCATCGCTCG TGCCAGCTAC AAGATACTGG CTACAGAGCA CGAGGCGCTC 
ATGCAGAAGT ACTTGCACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAACAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGGTAACT 
ATTACAAACC TGATCAAGGA CAATGACCGT GTTCAATCCA GCAACAAGGC CGCTTCTGCT 
GATGATGAGG ACAGTGACAT CAAGAAAATT AAGAAGGTTC AGAGCTTCCT TCGGGGATGG 
CTGTGCCGTC GAAAGTGGAA GAACATCATC CAGGACTACA TCCGGTCTCC CCACGCGGAC 
AGCATGCGCA AGAGGAACCA GGTGGTATTC AGCATGCTGG AGGCGGAGGC TGAGTACGTG 
CAGCAGCTAC ACATCCTTGT CAACAACTTC CTGCGCCCAC TGCGCATGGC CGCCAGCTCT 
AAGAAACCCC CTATAACACA CGACGACGTC AGCAGTATCT TTCTGAACAG CGAGACCATC 
ATGTTCCTGC ATCAGATCTT CTACCAAGGC CTGAAGGCCC GTATCTCTAG CTGGCCCACC 
CTGGTGCTGG CGGACCTGTT CGACATCCTG CTGCCCATGC TGAACATCTA CCAGGAGTTC 
GTCCGCAACC ACCAGTACAG CCTCCAGATC CTTGCTCACT GCAAGCAAAA CCGGGACTTT 
GACAAGCTCC TCAAGCAGTA TGAGGCCAAG CCAGACTGCG AGGAGCGCAC ACTGGAGACC 
TTCCTCACCT ACCCAATGTT CCAGATCCCC AGGTACATCC TGACACTCCA TGAGCTGCTG 
GCCCACACAC CCCATGAGCA TGTGGAGCGT AACAGCCTGG ACTATGCCAA ATCCAAACTA 
GAGGAACTGT CCAGGATCAT GCATGACGAA GTCAGTGAGA CCGAAAACAT CCGCAAAAAC 
CTGGCCATTG AGCGTATGAT CACTGAGGGC TGTGAGATCC TCCTGGACAC CAGCCAGACC 
TTCGTGCGCC AAGGTTCCCT CATGCAGGTG TCCTTGTCAG AAAAGAGCAA GTCCTCTAGG 
GGCCGCCTGG GGTCTCTGTC CATTAAGAAA GAGGGTGAGC GCCAGTGTTT CCTGTTCTCC 
AAGCATCTCA TCATCTGCAC CAGAGGCTCT GGTGGGAAAC TGCACCTAAC CAAGAATGGA 
GTCATATCCC TCATTGACTG CACCTTACTG GATGAACCCG AAAACCTGGA CGACGAAGCC 
AAAGGAGCTG GACCAGAGAT AGAGCACCTG GAGTTTAAGA TCGGGGTGGA GCCAAAGGAC 
TCCCCGCCCT TCACCGTCAT CCTGGTGGCC TCATCCAGGC AGGAGAAGGC AGCATGGACC 
AGTGACATCA TCCAGTGCGT GGATAATATC CGCTGCAATG GACTCATGAT GAATGCCTTC 
GAAGAAAATT CCAAGGTCAC CGTGCCTCAG ATGATCAAGT CTGACGCTTC CTTATATTGT 
GATGATGTTG ACATTCGCTT CAGCAAGACC ATGAATTCTT GCAAAGTGCT TCAGATCCGC 
TATGCCAGCG TGGAGCGCCT GCTGGAGCGC CTGACCGATC TTCGCTTCCT GAGCATTGAC 
TTTCTCAACA CCTTCCTGCA TTCCTATCGA GTCTTCACCA CTGCTATGGT GGTCCTGGAC 
AAGCTAATCA ACATCTACAG AAAACCCATG AGTGCCATTC CTGCCAGGTC CCTGGAACTC 
TTGTTTTCCA GCAGCCACAA CGCCAAGCTT CTGTACGGAG ATGCCCCCAA ATCCCCTCGC 
GCCAGCCGCA AGTTCTCCTC GCCGCCGCCC TTGGCCATCG GGACATCGTC CCCGAGCCGC 
CGTCGGAAGC TGTCCCTCAA CATCCCCATC ATCACAGGTG GCAAGGCACT GGAACTGGCT 
TCACTTGGGT GCAGCTCCGA TAGCTACGCC AACATACACT CACCCATATC CCCCTTCGGC 
AAAACCACAC TGGACACTGG CAAGCTCTGT ATGGCCAGCA GCCTACCCAA GACTCCAGAG 
GAGATTGACG TGCCTACCAC AATTCCAGAA AAGCCTGGGG AGCTCTCAGC CTCCAGAAAG 
CAGAGCTCTG ACATCCTGAA AGGAGAGTCT GATGGCGACC AGAACCACAG TGATGAAGAC 
AACACAGAAG TGTCGCCAGT TAAATCTCCA CCAACACCGA AGTCGTTCTT AAACAGAACC 
ATCACAGAGT TCCCATTCTT TAACTACAAC AACGGAGTCC TGATGACCAC GTGTCGCGAT 
CTGGTGGACA ATAACCGCAG CACTCTCTCA GCTACTTCTG CCTTTGCCAT AGCGACTGCA 
GGAGCCAATG AAGGCCCCTC AAACAAGGAG GTCTTTCGAA GGATGTCTTT GGCCAACACA 
GGCTTTTCCT CTGACCAGAG AAATATCGAC AAGGAGTTTG TGATCCGCAG AGCTGCCACC 
AATCGAGTGT TGAATGTGCT CCGCCACTGG GTCACCAAGC ACACCCAGGA CTTTGATACC 
GACGATACGC TCAAATACAG GGTGATCTGC TTCTTGGAGG AGGTCATGCA TGACCCAGAC 
CTTCTGACCC AAGAGCGAAA GGCAGCAGCC AACATCATCA GGACTCTGAC CCTAGAAGAA 
ACAACTGAAC AACATAGCAT GCTGGAAGAA GTCATACTGA TGACGGAGGG TGTGAAGACT 
GAGCCCTTCG AAAGCCACCC AGCCCTGGAG ATAGCAGAGC AGCTGACCCT ACTGGATCAC 
CTTGTCTTCA AGAGTATTCC TTATGAAGAG TTCTTTGGCC AGGGCTGGAT GAAGATGGAG 
AAGTATGAAA GGACACCTTA CATTATGAAA ACCACCAAAC ACTTCAACCA TGTCAGCAAC 
TTCATTGCTT CAGAAATTAT CCGAAACGAG GACATCAACG CAAGGGCAAG CGCCATCGAG 
AAGTGGGTGG CCGTGGCCGA CATATGTCGC TGCCTGCACA ACTACAATGC TGTGCTGGAG 
ATCACCTCCT CCATCAACCG CAGCGCCATC TTCCGACTCA AAAAGACATG GCTCAAAGTT 
TCTAAGCAGA CGAAATCTTT GCTTGACAAG CTCCAAAAGC TTGTGTCATC AGATGGCAGA 
TTTAAGAACC TCAGAGAGGC TTTGAGAAAT TGCGATCCAC CTTGTGTCCC TTACCTGGGG 
ATGTATCTCA CCGACTTGGT GTTCATCGAG GAGGGGACAC CCAATTACAC AGAGGACGGC 
CTGGTCAACT TCTCCAAGAT GAGAATGATC TCCCATATTA TCCGAGAGAT TCGCCAATTT 
CAGCAGACGA CCTACAAAAT CGAGCCCCAG CCAAAGGTAA TTCAGTACTT ACTGGATGAA 
TCTTTCATGT TGGACGAAGA GAGTCTGTAT GAGTCTTCGC TCCTAATTGA ACCAAAACTC 
CCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf1 ( XM_021206938.1 ) cDNA ORF clone, Mus pahari(shrew mouse) -> XP_021062597.1 Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X1, mRNA.

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CloneID	OMv47899
Clone ID Related Accession (Same CDS sequence)	XM_021206938.2 , XM_021206938.1
Accession Version	XM_021206938.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-18
Organism	Mus pahari(shrew mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034599.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus pahari Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ACTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGCTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCCG CCATCGCTCG TGCCAGCTAC AAGATACTGG CTACAGAGCA CGAGGCGCTC 
ATGCAGAAGT ACTTGCACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAACAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGGTAACT 
ATTACAAACC TGATCAAGGA CAATGACCGT GTTCAATCCA GCAACAAGGC CGCTTCTGCT 
GATGATGAGG ACAGTGACAT CAAGAAAATT AAGAAGGTTC AGAGCTTCCT TCGGGGATGG 
CTGTGCCGTC GAAAGTGGAA GAACATCATC CAGGACTACA TCCGGTCTCC CCACGCGGAC 
AGCATGCGCA AGAGGAACCA GGTGGTATTC AGCATGCTGG AGGCGGAGGC TGAGTACGTG 
CAGCAGCTAC ACATCCTTGT CAACAACTTC CTGCGCCCAC TGCGCATGGC CGCCAGCTCT 
AAGAAACCCC CTATAACACA CGACGACGTC AGCAGTATCT TTCTGAACAG CGAGACCATC 
ATGTTCCTGC ATCAGATCTT CTACCAAGGC CTGAAGGCCC GTATCTCTAG CTGGCCCACC 
CTGGTGCTGG CGGACCTGTT CGACATCCTG CTGCCCATGC TGAACATCTA CCAGGAGTTC 
GTCCGCAACC ACCAGTACAG CCTCCAGATC CTTGCTCACT GCAAGCAAAA CCGGGACTTT 
GACAAGCTCC TCAAGCAGTA TGAGGCCAAG CCAGACTGCG AGGAGCGCAC ACTGGAGACC 
TTCCTCACCT ACCCAATGTT CCAGATCCCC AGGTACATCC TGACACTCCA TGAGCTGCTG 
GCCCACACAC CCCATGAGCA TGTGGAGCGT AACAGCCTGG ACTATGCCAA ATCCAAACTA 
GAGGAACTGT CCAGGATCAT GCATGACGAA GTCAGTGAGA CCGAAAACAT CCGCAAAAAC 
CTGGCCATTG AGCGTATGAT CACTGAGGGC TGTGAGATCC TCCTGGACAC CAGCCAGACC 
TTCGTGCGCC AAGGTTCCCT CATGCAGGTG TCCTTGTCAG AAAAGAGCAA GTCCTCTAGG 
GGCCGCCTGG GGTCTCTGTC CATTAAGAAA GAGGGTGAGC GCCAGTGTTT CCTGTTCTCC 
AAGCATCTCA TCATCTGCAC CAGAGGCTCT GGTGGGAAAC TGCACCTAAC CAAGAATGGA 
GTCATATCCC TCATTGACTG CACCTTACTG GATGAACCCG AAAACCTGGA CGACGAAGCC 
AAAGGAGCTG GACCAGAGAT AGAGCACCTG GAGTTTAAGA TCGGGGTGGA GCCAAAGGAC 
TCCCCGCCCT TCACCGTCAT CCTGGTGGCC TCATCCAGGC AGGAGAAGGC AGCATGGACC 
AGTGACATCA TCCAGTGCGT GGATAATATC CGCTGCAATG GACTCATGAT GAATGCCTTC 
GAAGAAAATT CCAAGGTCAC CGTGCCTCAG ATGATCAAGT CTGACGCTTC CTTATATTGT 
GATGATGTTG ACATTCGCTT CAGCAAGACC ATGAATTCTT GCAAAGTGCT TCAGATCCGC 
TATGCCAGCG TGGAGCGCCT GCTGGAGCGC CTGACCGATC TTCGCTTCCT GAGCATTGAC 
TTTCTCAACA CCTTCCTGCA TTCCTATCGA GTCTTCACCA CTGCTATGGT GGTCCTGGAC 
AAGCTAATCA ACATCTACAG AAAACCCATG AGTGCCATTC CTGCCAGGTC CCTGGAACTC 
TTGTTTTCCA GCAGCCACAA CGCCAAGCTT CTGTACGGAG ATGCCCCCAA ATCCCCTCGC 
GCCAGCCGCA AGTTCTCCTC GCCGCCGCCC TTGGCCATCG GGACATCGTC CCCGAGCCGC 
CGTCGGAAGC TGTCCCTCAA CATCCCCATC ATCACAGGTG GCAAGGCACT GGAACTGGCT 
TCACTTGGGT GCAGCTCCGA TAGCTACGCC AACATACACT CACCCATATC CCCCTTCGGC 
AAAACCACAC TGGACACTGG CAAGCTCTGT ATGGCCAGCA GCCTACCCAA GACTCCAGAG 
GAGATTGACG TGCCTACCAC AATTCCAGAA AAGCCTGGGG AGCTCTCAGC CTCCAGAAAG 
CAGAGCTCTG ACATCCTGAA AGGAGAGTCT GATGGCGACC AGAACCACAG TGATGAAGAC 
AACACAGAAG TGTCGCCAGT TAAATCTCCA CCAACACCGA AGTCGTTCTT AAACAGAACC 
ATCACAGAGT TCCCATTCTT TAACTACAAC AACGGAGTCC TGATGACCAC GTGTCGCGAT 
CTGGTGGACA ATAACCGCAG CACTCTCTCA GCTACTTCTG CCTTTGCCAT AGCGACTGCA 
GGAGCCAATG AAGGCCCCTC AAACAAGGAG GTCTTTCGAA GGATGTCTTT GGCCAACACA 
GGCTTTTCCT CTGACCAGAG AAATATCGAC AAGGAGTTTG TGATCCGCAG AGCTGCCACC 
AATCGAGTGT TGAATGTGCT CCGCCACTGG GTCACCAAGC ACACCCAGGA CTTTGATACC 
GACGATACGC TCAAATACAG GGTGATCTGC TTCTTGGAGG AGGTCATGCA TGACCCAGAC 
CTTCTGACCC AAGAGCGAAA GGCAGCAGCC AACATCATCA GGACTCTGAC CCTAGAAGAA 
ACAACTGAAC AACATAGCAT GCTGGAAGAA GTCATACTGA TGACGGAGGG TGTGAAGACT 
GAGCCCTTCG AAAGCCACCC AGCCCTGGAG ATAGCAGAGC AGCTGACCCT ACTGGATCAC 
CTTGTCTTCA AGAGTATTCC TTATGAAGAG TTCTTTGGCC AGGGCTGGAT GAAGATGGAG 
AAGTATGAAA GGACACCTTA CATTATGAAA ACCACCAAAC ACTTCAACCA TGTCAGCAAC 
TTCATTGCTT CAGAAATTAT CCGAAACGAG GACATCAACG CAAGGGCAAG CGCCATCGAG 
AAGTGGGTGG CCGTGGCCGA CATATGTCGC TGCCTGCACA ACTACAATGC TGTGCTGGAG 
ATCACCTCCT CCATCAACCG CAGCGCCATC TTCCGACTCA AAAAGACATG GCTCAAAGTT 
TCTAAGCAGA CGAAATCTTT GCTTGACAAG CTCCAAAAGC TTGTGTCATC AGATGGCAGA 
TTTAAGAACC TCAGAGAGGC TTTGAGAAAT TGCGATCCAC CTTGTGTCCC TTACCTGGGG 
ATGTATCTCA CCGACTTGGT GTTCATCGAG GAGGGGACAC CCAATTACAC AGAGGACGGC 
CTGGTCAACT TCTCCAAGAT GAGAATGATC TCCCATATTA TCCGAGAGAT TCGCCAATTT 
CAGCAGACGA CCTACAAAAT CGAGCCCCAG CCAAAGGTAA TTCAGTACTT ACTGGATGAA 
TCTTTCATGT TGGACGAAGA GAGTCTGTAT GAGTCTTCGC TCCTAATTGA ACCAAAACTC 
CCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021062597.1
CDS	220..4008
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQQHYFTVNFSNDSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKTEVTITNLIKDNDRVQSSNKAASADDEDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLMQVSLSEKSKSSRGRLGSLSIKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLDEPENLDDEAKGAGPEIEHLEFKIGVEPKDSPPFTVILVASSRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAMVVLDKLINIYRKPMSAIPARSLELLFSSSHNAKLLYGDAPKSPRASRKFSSPPPLAIGTSSPSRRRKLSLNIPIITGGKALELASLGCSSDSYANIHSPISPFGKTTLDTGKLCMASSLPKTPEEIDVPTTIPEKPGELSASRKQSSDILKGESDGDQNHSDEDNTEVSPVKSPPTPKSFLNRTITEFPFFNYNNGVLMTTCRDLVDNNRSTLSATSAFAIATAGANEGPSNKEVFRRMSLANTGFSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFLEEVMHDPDLLTQERKAAANIIRTLTLEETTEQHSMLEEVILMTEGVKTEPFESHPALEIAEQLTLLDHLVFKSIPYEEFFGQGWMKMEKYERTPYIMKTTKHFNHVSNFIASEIIRNEDINARASAIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLLDKLQKLVSSDGRFKNLREALRNCDPPCVPYLGMYLTDLVFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIEPQPKVIQYLLDESFMLDEESLYESSLLIEPKLPT

Target ORF information:

RefSeq Version	XM_021206938.1
Organism	Mus pahari(shrew mouse)
Definition	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021206938.1

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ACTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGCTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCCG CCATCGCTCG TGCCAGCTAC AAGATACTGG CTACAGAGCA CGAGGCGCTC 
ATGCAGAAGT ACTTGCACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAACAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGGTAACT 
ATTACAAACC TGATCAAGGA CAATGACCGT GTTCAATCCA GCAACAAGGC CGCTTCTGCT 
GATGATGAGG ACAGTGACAT CAAGAAAATT AAGAAGGTTC AGAGCTTCCT TCGGGGATGG 
CTGTGCCGTC GAAAGTGGAA GAACATCATC CAGGACTACA TCCGGTCTCC CCACGCGGAC 
AGCATGCGCA AGAGGAACCA GGTGGTATTC AGCATGCTGG AGGCGGAGGC TGAGTACGTG 
CAGCAGCTAC ACATCCTTGT CAACAACTTC CTGCGCCCAC TGCGCATGGC CGCCAGCTCT 
AAGAAACCCC CTATAACACA CGACGACGTC AGCAGTATCT TTCTGAACAG CGAGACCATC 
ATGTTCCTGC ATCAGATCTT CTACCAAGGC CTGAAGGCCC GTATCTCTAG CTGGCCCACC 
CTGGTGCTGG CGGACCTGTT CGACATCCTG CTGCCCATGC TGAACATCTA CCAGGAGTTC 
GTCCGCAACC ACCAGTACAG CCTCCAGATC CTTGCTCACT GCAAGCAAAA CCGGGACTTT 
GACAAGCTCC TCAAGCAGTA TGAGGCCAAG CCAGACTGCG AGGAGCGCAC ACTGGAGACC 
TTCCTCACCT ACCCAATGTT CCAGATCCCC AGGTACATCC TGACACTCCA TGAGCTGCTG 
GCCCACACAC CCCATGAGCA TGTGGAGCGT AACAGCCTGG ACTATGCCAA ATCCAAACTA 
GAGGAACTGT CCAGGATCAT GCATGACGAA GTCAGTGAGA CCGAAAACAT CCGCAAAAAC 
CTGGCCATTG AGCGTATGAT CACTGAGGGC TGTGAGATCC TCCTGGACAC CAGCCAGACC 
TTCGTGCGCC AAGGTTCCCT CATGCAGGTG TCCTTGTCAG AAAAGAGCAA GTCCTCTAGG 
GGCCGCCTGG GGTCTCTGTC CATTAAGAAA GAGGGTGAGC GCCAGTGTTT CCTGTTCTCC 
AAGCATCTCA TCATCTGCAC CAGAGGCTCT GGTGGGAAAC TGCACCTAAC CAAGAATGGA 
GTCATATCCC TCATTGACTG CACCTTACTG GATGAACCCG AAAACCTGGA CGACGAAGCC 
AAAGGAGCTG GACCAGAGAT AGAGCACCTG GAGTTTAAGA TCGGGGTGGA GCCAAAGGAC 
TCCCCGCCCT TCACCGTCAT CCTGGTGGCC TCATCCAGGC AGGAGAAGGC AGCATGGACC 
AGTGACATCA TCCAGTGCGT GGATAATATC CGCTGCAATG GACTCATGAT GAATGCCTTC 
GAAGAAAATT CCAAGGTCAC CGTGCCTCAG ATGATCAAGT CTGACGCTTC CTTATATTGT 
GATGATGTTG ACATTCGCTT CAGCAAGACC ATGAATTCTT GCAAAGTGCT TCAGATCCGC 
TATGCCAGCG TGGAGCGCCT GCTGGAGCGC CTGACCGATC TTCGCTTCCT GAGCATTGAC 
TTTCTCAACA CCTTCCTGCA TTCCTATCGA GTCTTCACCA CTGCTATGGT GGTCCTGGAC 
AAGCTAATCA ACATCTACAG AAAACCCATG AGTGCCATTC CTGCCAGGTC CCTGGAACTC 
TTGTTTTCCA GCAGCCACAA CGCCAAGCTT CTGTACGGAG ATGCCCCCAA ATCCCCTCGC 
GCCAGCCGCA AGTTCTCCTC GCCGCCGCCC TTGGCCATCG GGACATCGTC CCCGAGCCGC 
CGTCGGAAGC TGTCCCTCAA CATCCCCATC ATCACAGGTG GCAAGGCACT GGAACTGGCT 
TCACTTGGGT GCAGCTCCGA TAGCTACGCC AACATACACT CACCCATATC CCCCTTCGGC 
AAAACCACAC TGGACACTGG CAAGCTCTGT ATGGCCAGCA GCCTACCCAA GACTCCAGAG 
GAGATTGACG TGCCTACCAC AATTCCAGAA AAGCCTGGGG AGCTCTCAGC CTCCAGAAAG 
CAGAGCTCTG ACATCCTGAA AGGAGAGTCT GATGGCGACC AGAACCACAG TGATGAAGAC 
AACACAGAAG TGTCGCCAGT TAAATCTCCA CCAACACCGA AGTCGTTCTT AAACAGAACC 
ATCACAGAGT TCCCATTCTT TAACTACAAC AACGGAGTCC TGATGACCAC GTGTCGCGAT 
CTGGTGGACA ATAACCGCAG CACTCTCTCA GCTACTTCTG CCTTTGCCAT AGCGACTGCA 
GGAGCCAATG AAGGCCCCTC AAACAAGGAG GTCTTTCGAA GGATGTCTTT GGCCAACACA 
GGCTTTTCCT CTGACCAGAG AAATATCGAC AAGGAGTTTG TGATCCGCAG AGCTGCCACC 
AATCGAGTGT TGAATGTGCT CCGCCACTGG GTCACCAAGC ACACCCAGGA CTTTGATACC 
GACGATACGC TCAAATACAG GGTGATCTGC TTCTTGGAGG AGGTCATGCA TGACCCAGAC 
CTTCTGACCC AAGAGCGAAA GGCAGCAGCC AACATCATCA GGACTCTGAC CCTAGAAGAA 
ACAACTGAAC AACATAGCAT GCTGGAAGAA GTCATACTGA TGACGGAGGG TGTGAAGACT 
GAGCCCTTCG AAAGCCACCC AGCCCTGGAG ATAGCAGAGC AGCTGACCCT ACTGGATCAC 
CTTGTCTTCA AGAGTATTCC TTATGAAGAG TTCTTTGGCC AGGGCTGGAT GAAGATGGAG 
AAGTATGAAA GGACACCTTA CATTATGAAA ACCACCAAAC ACTTCAACCA TGTCAGCAAC 
TTCATTGCTT CAGAAATTAT CCGAAACGAG GACATCAACG CAAGGGCAAG CGCCATCGAG 
AAGTGGGTGG CCGTGGCCGA CATATGTCGC TGCCTGCACA ACTACAATGC TGTGCTGGAG 
ATCACCTCCT CCATCAACCG CAGCGCCATC TTCCGACTCA AAAAGACATG GCTCAAAGTT 
TCTAAGCAGA CGAAATCTTT GCTTGACAAG CTCCAAAAGC TTGTGTCATC AGATGGCAGA 
TTTAAGAACC TCAGAGAGGC TTTGAGAAAT TGCGATCCAC CTTGTGTCCC TTACCTGGGG 
ATGTATCTCA CCGACTTGGT GTTCATCGAG GAGGGGACAC CCAATTACAC AGAGGACGGC 
CTGGTCAACT TCTCCAAGAT GAGAATGATC TCCCATATTA TCCGAGAGAT TCGCCAATTT 
CAGCAGACGA CCTACAAAAT CGAGCCCCAG CCAAAGGTAA TTCAGTACTT ACTGGATGAA 
TCTTTCATGT TGGACGAAGA GAGTCTGTAT GAGTCTTCGC TCCTAATTGA ACCAAAACTC 
CCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf1 ( XM_021206939.1 ) cDNA ORF clone, Mus pahari(shrew mouse) -> XP_021062598.1 Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMv47900
Clone ID Related Accession (Same CDS sequence)	XM_021206939.1 , XM_021206939.2
Accession Version	XM_021206939.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-18
Organism	Mus pahari(shrew mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034599.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus pahari Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ACTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGCTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCCG CCATCGCTCG TGCCAGCTAC AAGATACTGG CTACAGAGCA CGAGGCGCTC 
ATGCAGAAGT ACTTGCACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAACAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGATTACA 
AACCTGATCA AGGACAATGA CCGTGTTCAA TCCAGCAACA AGGCCGCTTC TGCTGATGAT 
GAGGACAGTG ACATCAAGAA AATTAAGAAG GTTCAGAGCT TCCTTCGGGG ATGGCTGTGC 
CGTCGAAAGT GGAAGAACAT CATCCAGGAC TACATCCGGT CTCCCCACGC GGACAGCATG 
CGCAAGAGGA ACCAGGTGGT ATTCAGCATG CTGGAGGCGG AGGCTGAGTA CGTGCAGCAG 
CTACACATCC TTGTCAACAA CTTCCTGCGC CCACTGCGCA TGGCCGCCAG CTCTAAGAAA 
CCCCCTATAA CACACGACGA CGTCAGCAGT ATCTTTCTGA ACAGCGAGAC CATCATGTTC 
CTGCATCAGA TCTTCTACCA AGGCCTGAAG GCCCGTATCT CTAGCTGGCC CACCCTGGTG 
CTGGCGGACC TGTTCGACAT CCTGCTGCCC ATGCTGAACA TCTACCAGGA GTTCGTCCGC 
AACCACCAGT ACAGCCTCCA GATCCTTGCT CACTGCAAGC AAAACCGGGA CTTTGACAAG 
CTCCTCAAGC AGTATGAGGC CAAGCCAGAC TGCGAGGAGC GCACACTGGA GACCTTCCTC 
ACCTACCCAA TGTTCCAGAT CCCCAGGTAC ATCCTGACAC TCCATGAGCT GCTGGCCCAC 
ACACCCCATG AGCATGTGGA GCGTAACAGC CTGGACTATG CCAAATCCAA ACTAGAGGAA 
CTGTCCAGGA TCATGCATGA CGAAGTCAGT GAGACCGAAA ACATCCGCAA AAACCTGGCC 
ATTGAGCGTA TGATCACTGA GGGCTGTGAG ATCCTCCTGG ACACCAGCCA GACCTTCGTG 
CGCCAAGGTT CCCTCATGCA GGTGTCCTTG TCAGAAAAGA GCAAGTCCTC TAGGGGCCGC 
CTGGGGTCTC TGTCCATTAA GAAAGAGGGT GAGCGCCAGT GTTTCCTGTT CTCCAAGCAT 
CTCATCATCT GCACCAGAGG CTCTGGTGGG AAACTGCACC TAACCAAGAA TGGAGTCATA 
TCCCTCATTG ACTGCACCTT ACTGGATGAA CCCGAAAACC TGGACGACGA AGCCAAAGGA 
GCTGGACCAG AGATAGAGCA CCTGGAGTTT AAGATCGGGG TGGAGCCAAA GGACTCCCCG 
CCCTTCACCG TCATCCTGGT GGCCTCATCC AGGCAGGAGA AGGCAGCATG GACCAGTGAC 
ATCATCCAGT GCGTGGATAA TATCCGCTGC AATGGACTCA TGATGAATGC CTTCGAAGAA 
AATTCCAAGG TCACCGTGCC TCAGATGATC AAGTCTGACG CTTCCTTATA TTGTGATGAT 
GTTGACATTC GCTTCAGCAA GACCATGAAT TCTTGCAAAG TGCTTCAGAT CCGCTATGCC 
AGCGTGGAGC GCCTGCTGGA GCGCCTGACC GATCTTCGCT TCCTGAGCAT TGACTTTCTC 
AACACCTTCC TGCATTCCTA TCGAGTCTTC ACCACTGCTA TGGTGGTCCT GGACAAGCTA 
ATCAACATCT ACAGAAAACC CATGAGTGCC ATTCCTGCCA GGTCCCTGGA ACTCTTGTTT 
TCCAGCAGCC ACAACGCCAA GCTTCTGTAC GGAGATGCCC CCAAATCCCC TCGCGCCAGC 
CGCAAGTTCT CCTCGCCGCC GCCCTTGGCC ATCGGGACAT CGTCCCCGAG CCGCCGTCGG 
AAGCTGTCCC TCAACATCCC CATCATCACA GGTGGCAAGG CACTGGAACT GGCTTCACTT 
GGGTGCAGCT CCGATAGCTA CGCCAACATA CACTCACCCA TATCCCCCTT CGGCAAAACC 
ACACTGGACA CTGGCAAGCT CTGTATGGCC AGCAGCCTAC CCAAGACTCC AGAGGAGATT 
GACGTGCCTA CCACAATTCC AGAAAAGCCT GGGGAGCTCT CAGCCTCCAG AAAGCAGAGC 
TCTGACATCC TGAAAGGAGA GTCTGATGGC GACCAGAACC ACAGTGATGA AGACAACACA 
GAAGTGTCGC CAGTTAAATC TCCACCAACA CCGAAGTCGT TCTTAAACAG AACCATCACA 
GAGTTCCCAT TCTTTAACTA CAACAACGGA GTCCTGATGA CCACGTGTCG CGATCTGGTG 
GACAATAACC GCAGCACTCT CTCAGCTACT TCTGCCTTTG CCATAGCGAC TGCAGGAGCC 
AATGAAGGCC CCTCAAACAA GGAGGTCTTT CGAAGGATGT CTTTGGCCAA CACAGGCTTT 
TCCTCTGACC AGAGAAATAT CGACAAGGAG TTTGTGATCC GCAGAGCTGC CACCAATCGA 
GTGTTGAATG TGCTCCGCCA CTGGGTCACC AAGCACACCC AGGACTTTGA TACCGACGAT 
ACGCTCAAAT ACAGGGTGAT CTGCTTCTTG GAGGAGGTCA TGCATGACCC AGACCTTCTG 
ACCCAAGAGC GAAAGGCAGC AGCCAACATC ATCAGGACTC TGACCCTAGA AGAAACAACT 
GAACAACATA GCATGCTGGA AGAAGTCATA CTGATGACGG AGGGTGTGAA GACTGAGCCC 
TTCGAAAGCC ACCCAGCCCT GGAGATAGCA GAGCAGCTGA CCCTACTGGA TCACCTTGTC 
TTCAAGAGTA TTCCTTATGA AGAGTTCTTT GGCCAGGGCT GGATGAAGAT GGAGAAGTAT 
GAAAGGACAC CTTACATTAT GAAAACCACC AAACACTTCA ACCATGTCAG CAACTTCATT 
GCTTCAGAAA TTATCCGAAA CGAGGACATC AACGCAAGGG CAAGCGCCAT CGAGAAGTGG 
GTGGCCGTGG CCGACATATG TCGCTGCCTG CACAACTACA ATGCTGTGCT GGAGATCACC 
TCCTCCATCA ACCGCAGCGC CATCTTCCGA CTCAAAAAGA CATGGCTCAA AGTTTCTAAG 
CAGACGAAAT CTTTGCTTGA CAAGCTCCAA AAGCTTGTGT CATCAGATGG CAGATTTAAG 
AACCTCAGAG AGGCTTTGAG AAATTGCGAT CCACCTTGTG TCCCTTACCT GGGGATGTAT 
CTCACCGACT TGGTGTTCAT CGAGGAGGGG ACACCCAATT ACACAGAGGA CGGCCTGGTC 
AACTTCTCCA AGATGAGAAT GATCTCCCAT ATTATCCGAG AGATTCGCCA ATTTCAGCAG 
ACGACCTACA AAATCGAGCC CCAGCCAAAG GTAATTCAGT ACTTACTGGA TGAATCTTTC 
ATGTTGGACG AAGAGAGTCT GTATGAGTCT TCGCTCCTAA TTGAACCAAA ACTCCCCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021062598.1
CDS	1..3783
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQQHYFTVNFSNDSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKTEITNLIKDNDRVQSSNKAASADDEDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLMQVSLSEKSKSSRGRLGSLSIKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLDEPENLDDEAKGAGPEIEHLEFKIGVEPKDSPPFTVILVASSRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAMVVLDKLINIYRKPMSAIPARSLELLFSSSHNAKLLYGDAPKSPRASRKFSSPPPLAIGTSSPSRRRKLSLNIPIITGGKALELASLGCSSDSYANIHSPISPFGKTTLDTGKLCMASSLPKTPEEIDVPTTIPEKPGELSASRKQSSDILKGESDGDQNHSDEDNTEVSPVKSPPTPKSFLNRTITEFPFFNYNNGVLMTTCRDLVDNNRSTLSATSAFAIATAGANEGPSNKEVFRRMSLANTGFSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFLEEVMHDPDLLTQERKAAANIIRTLTLEETTEQHSMLEEVILMTEGVKTEPFESHPALEIAEQLTLLDHLVFKSIPYEEFFGQGWMKMEKYERTPYIMKTTKHFNHVSNFIASEIIRNEDINARASAIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLLDKLQKLVSSDGRFKNLREALRNCDPPCVPYLGMYLTDLVFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIEPQPKVIQYLLDESFMLDEESLYESSLLIEPKLPT

Target ORF information:

RefSeq Version	XM_021206939.1
Organism	Mus pahari(shrew mouse)
Definition	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021206939.1

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ACTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGCTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCCG CCATCGCTCG TGCCAGCTAC AAGATACTGG CTACAGAGCA CGAGGCGCTC 
ATGCAGAAGT ACTTGCACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAACAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGATTACA 
AACCTGATCA AGGACAATGA CCGTGTTCAA TCCAGCAACA AGGCCGCTTC TGCTGATGAT 
GAGGACAGTG ACATCAAGAA AATTAAGAAG GTTCAGAGCT TCCTTCGGGG ATGGCTGTGC 
CGTCGAAAGT GGAAGAACAT CATCCAGGAC TACATCCGGT CTCCCCACGC GGACAGCATG 
CGCAAGAGGA ACCAGGTGGT ATTCAGCATG CTGGAGGCGG AGGCTGAGTA CGTGCAGCAG 
CTACACATCC TTGTCAACAA CTTCCTGCGC CCACTGCGCA TGGCCGCCAG CTCTAAGAAA 
CCCCCTATAA CACACGACGA CGTCAGCAGT ATCTTTCTGA ACAGCGAGAC CATCATGTTC 
CTGCATCAGA TCTTCTACCA AGGCCTGAAG GCCCGTATCT CTAGCTGGCC CACCCTGGTG 
CTGGCGGACC TGTTCGACAT CCTGCTGCCC ATGCTGAACA TCTACCAGGA GTTCGTCCGC 
AACCACCAGT ACAGCCTCCA GATCCTTGCT CACTGCAAGC AAAACCGGGA CTTTGACAAG 
CTCCTCAAGC AGTATGAGGC CAAGCCAGAC TGCGAGGAGC GCACACTGGA GACCTTCCTC 
ACCTACCCAA TGTTCCAGAT CCCCAGGTAC ATCCTGACAC TCCATGAGCT GCTGGCCCAC 
ACACCCCATG AGCATGTGGA GCGTAACAGC CTGGACTATG CCAAATCCAA ACTAGAGGAA 
CTGTCCAGGA TCATGCATGA CGAAGTCAGT GAGACCGAAA ACATCCGCAA AAACCTGGCC 
ATTGAGCGTA TGATCACTGA GGGCTGTGAG ATCCTCCTGG ACACCAGCCA GACCTTCGTG 
CGCCAAGGTT CCCTCATGCA GGTGTCCTTG TCAGAAAAGA GCAAGTCCTC TAGGGGCCGC 
CTGGGGTCTC TGTCCATTAA GAAAGAGGGT GAGCGCCAGT GTTTCCTGTT CTCCAAGCAT 
CTCATCATCT GCACCAGAGG CTCTGGTGGG AAACTGCACC TAACCAAGAA TGGAGTCATA 
TCCCTCATTG ACTGCACCTT ACTGGATGAA CCCGAAAACC TGGACGACGA AGCCAAAGGA 
GCTGGACCAG AGATAGAGCA CCTGGAGTTT AAGATCGGGG TGGAGCCAAA GGACTCCCCG 
CCCTTCACCG TCATCCTGGT GGCCTCATCC AGGCAGGAGA AGGCAGCATG GACCAGTGAC 
ATCATCCAGT GCGTGGATAA TATCCGCTGC AATGGACTCA TGATGAATGC CTTCGAAGAA 
AATTCCAAGG TCACCGTGCC TCAGATGATC AAGTCTGACG CTTCCTTATA TTGTGATGAT 
GTTGACATTC GCTTCAGCAA GACCATGAAT TCTTGCAAAG TGCTTCAGAT CCGCTATGCC 
AGCGTGGAGC GCCTGCTGGA GCGCCTGACC GATCTTCGCT TCCTGAGCAT TGACTTTCTC 
AACACCTTCC TGCATTCCTA TCGAGTCTTC ACCACTGCTA TGGTGGTCCT GGACAAGCTA 
ATCAACATCT ACAGAAAACC CATGAGTGCC ATTCCTGCCA GGTCCCTGGA ACTCTTGTTT 
TCCAGCAGCC ACAACGCCAA GCTTCTGTAC GGAGATGCCC CCAAATCCCC TCGCGCCAGC 
CGCAAGTTCT CCTCGCCGCC GCCCTTGGCC ATCGGGACAT CGTCCCCGAG CCGCCGTCGG 
AAGCTGTCCC TCAACATCCC CATCATCACA GGTGGCAAGG CACTGGAACT GGCTTCACTT 
GGGTGCAGCT CCGATAGCTA CGCCAACATA CACTCACCCA TATCCCCCTT CGGCAAAACC 
ACACTGGACA CTGGCAAGCT CTGTATGGCC AGCAGCCTAC CCAAGACTCC AGAGGAGATT 
GACGTGCCTA CCACAATTCC AGAAAAGCCT GGGGAGCTCT CAGCCTCCAG AAAGCAGAGC 
TCTGACATCC TGAAAGGAGA GTCTGATGGC GACCAGAACC ACAGTGATGA AGACAACACA 
GAAGTGTCGC CAGTTAAATC TCCACCAACA CCGAAGTCGT TCTTAAACAG AACCATCACA 
GAGTTCCCAT TCTTTAACTA CAACAACGGA GTCCTGATGA CCACGTGTCG CGATCTGGTG 
GACAATAACC GCAGCACTCT CTCAGCTACT TCTGCCTTTG CCATAGCGAC TGCAGGAGCC 
AATGAAGGCC CCTCAAACAA GGAGGTCTTT CGAAGGATGT CTTTGGCCAA CACAGGCTTT 
TCCTCTGACC AGAGAAATAT CGACAAGGAG TTTGTGATCC GCAGAGCTGC CACCAATCGA 
GTGTTGAATG TGCTCCGCCA CTGGGTCACC AAGCACACCC AGGACTTTGA TACCGACGAT 
ACGCTCAAAT ACAGGGTGAT CTGCTTCTTG GAGGAGGTCA TGCATGACCC AGACCTTCTG 
ACCCAAGAGC GAAAGGCAGC AGCCAACATC ATCAGGACTC TGACCCTAGA AGAAACAACT 
GAACAACATA GCATGCTGGA AGAAGTCATA CTGATGACGG AGGGTGTGAA GACTGAGCCC 
TTCGAAAGCC ACCCAGCCCT GGAGATAGCA GAGCAGCTGA CCCTACTGGA TCACCTTGTC 
TTCAAGAGTA TTCCTTATGA AGAGTTCTTT GGCCAGGGCT GGATGAAGAT GGAGAAGTAT 
GAAAGGACAC CTTACATTAT GAAAACCACC AAACACTTCA ACCATGTCAG CAACTTCATT 
GCTTCAGAAA TTATCCGAAA CGAGGACATC AACGCAAGGG CAAGCGCCAT CGAGAAGTGG 
GTGGCCGTGG CCGACATATG TCGCTGCCTG CACAACTACA ATGCTGTGCT GGAGATCACC 
TCCTCCATCA ACCGCAGCGC CATCTTCCGA CTCAAAAAGA CATGGCTCAA AGTTTCTAAG 
CAGACGAAAT CTTTGCTTGA CAAGCTCCAA AAGCTTGTGT CATCAGATGG CAGATTTAAG 
AACCTCAGAG AGGCTTTGAG AAATTGCGAT CCACCTTGTG TCCCTTACCT GGGGATGTAT 
CTCACCGACT TGGTGTTCAT CGAGGAGGGG ACACCCAATT ACACAGAGGA CGGCCTGGTC 
AACTTCTCCA AGATGAGAAT GATCTCCCAT ATTATCCGAG AGATTCGCCA ATTTCAGCAG 
ACGACCTACA AAATCGAGCC CCAGCCAAAG GTAATTCAGT ACTTACTGGA TGAATCTTTC 
ATGTTGGACG AAGAGAGTCT GTATGAGTCT TCGCTCCTAA TTGAACCAAA ACTCCCCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf1 ( XM_021206939.2 ) cDNA ORF clone, Mus pahari(shrew mouse) -> XP_021062598.1 Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMv47900
Clone ID Related Accession (Same CDS sequence)	XM_021206939.1 , XM_021206939.2
Accession Version	XM_021206939.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Mus pahari(shrew mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034599.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 18, 2019 this sequence version replaced XM_021206939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus pahari Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ACTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGCTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCCG CCATCGCTCG TGCCAGCTAC AAGATACTGG CTACAGAGCA CGAGGCGCTC 
ATGCAGAAGT ACTTGCACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAACAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGATTACA 
AACCTGATCA AGGACAATGA CCGTGTTCAA TCCAGCAACA AGGCCGCTTC TGCTGATGAT 
GAGGACAGTG ACATCAAGAA AATTAAGAAG GTTCAGAGCT TCCTTCGGGG ATGGCTGTGC 
CGTCGAAAGT GGAAGAACAT CATCCAGGAC TACATCCGGT CTCCCCACGC GGACAGCATG 
CGCAAGAGGA ACCAGGTGGT ATTCAGCATG CTGGAGGCGG AGGCTGAGTA CGTGCAGCAG 
CTACACATCC TTGTCAACAA CTTCCTGCGC CCACTGCGCA TGGCCGCCAG CTCTAAGAAA 
CCCCCTATAA CACACGACGA CGTCAGCAGT ATCTTTCTGA ACAGCGAGAC CATCATGTTC 
CTGCATCAGA TCTTCTACCA AGGCCTGAAG GCCCGTATCT CTAGCTGGCC CACCCTGGTG 
CTGGCGGACC TGTTCGACAT CCTGCTGCCC ATGCTGAACA TCTACCAGGA GTTCGTCCGC 
AACCACCAGT ACAGCCTCCA GATCCTTGCT CACTGCAAGC AAAACCGGGA CTTTGACAAG 
CTCCTCAAGC AGTATGAGGC CAAGCCAGAC TGCGAGGAGC GCACACTGGA GACCTTCCTC 
ACCTACCCAA TGTTCCAGAT CCCCAGGTAC ATCCTGACAC TCCATGAGCT GCTGGCCCAC 
ACACCCCATG AGCATGTGGA GCGTAACAGC CTGGACTATG CCAAATCCAA ACTAGAGGAA 
CTGTCCAGGA TCATGCATGA CGAAGTCAGT GAGACCGAAA ACATCCGCAA AAACCTGGCC 
ATTGAGCGTA TGATCACTGA GGGCTGTGAG ATCCTCCTGG ACACCAGCCA GACCTTCGTG 
CGCCAAGGTT CCCTCATGCA GGTGTCCTTG TCAGAAAAGA GCAAGTCCTC TAGGGGCCGC 
CTGGGGTCTC TGTCCATTAA GAAAGAGGGT GAGCGCCAGT GTTTCCTGTT CTCCAAGCAT 
CTCATCATCT GCACCAGAGG CTCTGGTGGG AAACTGCACC TAACCAAGAA TGGAGTCATA 
TCCCTCATTG ACTGCACCTT ACTGGATGAA CCCGAAAACC TGGACGACGA AGCCAAAGGA 
GCTGGACCAG AGATAGAGCA CCTGGAGTTT AAGATCGGGG TGGAGCCAAA GGACTCCCCG 
CCCTTCACCG TCATCCTGGT GGCCTCATCC AGGCAGGAGA AGGCAGCATG GACCAGTGAC 
ATCATCCAGT GCGTGGATAA TATCCGCTGC AATGGACTCA TGATGAATGC CTTCGAAGAA 
AATTCCAAGG TCACCGTGCC TCAGATGATC AAGTCTGACG CTTCCTTATA TTGTGATGAT 
GTTGACATTC GCTTCAGCAA GACCATGAAT TCTTGCAAAG TGCTTCAGAT CCGCTATGCC 
AGCGTGGAGC GCCTGCTGGA GCGCCTGACC GATCTTCGCT TCCTGAGCAT TGACTTTCTC 
AACACCTTCC TGCATTCCTA TCGAGTCTTC ACCACTGCTA TGGTGGTCCT GGACAAGCTA 
ATCAACATCT ACAGAAAACC CATGAGTGCC ATTCCTGCCA GGTCCCTGGA ACTCTTGTTT 
TCCAGCAGCC ACAACGCCAA GCTTCTGTAC GGAGATGCCC CCAAATCCCC TCGCGCCAGC 
CGCAAGTTCT CCTCGCCGCC GCCCTTGGCC ATCGGGACAT CGTCCCCGAG CCGCCGTCGG 
AAGCTGTCCC TCAACATCCC CATCATCACA GGTGGCAAGG CACTGGAACT GGCTTCACTT 
GGGTGCAGCT CCGATAGCTA CGCCAACATA CACTCACCCA TATCCCCCTT CGGCAAAACC 
ACACTGGACA CTGGCAAGCT CTGTATGGCC AGCAGCCTAC CCAAGACTCC AGAGGAGATT 
GACGTGCCTA CCACAATTCC AGAAAAGCCT GGGGAGCTCT CAGCCTCCAG AAAGCAGAGC 
TCTGACATCC TGAAAGGAGA GTCTGATGGC GACCAGAACC ACAGTGATGA AGACAACACA 
GAAGTGTCGC CAGTTAAATC TCCACCAACA CCGAAGTCGT TCTTAAACAG AACCATCACA 
GAGTTCCCAT TCTTTAACTA CAACAACGGA GTCCTGATGA CCACGTGTCG CGATCTGGTG 
GACAATAACC GCAGCACTCT CTCAGCTACT TCTGCCTTTG CCATAGCGAC TGCAGGAGCC 
AATGAAGGCC CCTCAAACAA GGAGGTCTTT CGAAGGATGT CTTTGGCCAA CACAGGCTTT 
TCCTCTGACC AGAGAAATAT CGACAAGGAG TTTGTGATCC GCAGAGCTGC CACCAATCGA 
GTGTTGAATG TGCTCCGCCA CTGGGTCACC AAGCACACCC AGGACTTTGA TACCGACGAT 
ACGCTCAAAT ACAGGGTGAT CTGCTTCTTG GAGGAGGTCA TGCATGACCC AGACCTTCTG 
ACCCAAGAGC GAAAGGCAGC AGCCAACATC ATCAGGACTC TGACCCTAGA AGAAACAACT 
GAACAACATA GCATGCTGGA AGAAGTCATA CTGATGACGG AGGGTGTGAA GACTGAGCCC 
TTCGAAAGCC ACCCAGCCCT GGAGATAGCA GAGCAGCTGA CCCTACTGGA TCACCTTGTC 
TTCAAGAGTA TTCCTTATGA AGAGTTCTTT GGCCAGGGCT GGATGAAGAT GGAGAAGTAT 
GAAAGGACAC CTTACATTAT GAAAACCACC AAACACTTCA ACCATGTCAG CAACTTCATT 
GCTTCAGAAA TTATCCGAAA CGAGGACATC AACGCAAGGG CAAGCGCCAT CGAGAAGTGG 
GTGGCCGTGG CCGACATATG TCGCTGCCTG CACAACTACA ATGCTGTGCT GGAGATCACC 
TCCTCCATCA ACCGCAGCGC CATCTTCCGA CTCAAAAAGA CATGGCTCAA AGTTTCTAAG 
CAGACGAAAT CTTTGCTTGA CAAGCTCCAA AAGCTTGTGT CATCAGATGG CAGATTTAAG 
AACCTCAGAG AGGCTTTGAG AAATTGCGAT CCACCTTGTG TCCCTTACCT GGGGATGTAT 
CTCACCGACT TGGTGTTCAT CGAGGAGGGG ACACCCAATT ACACAGAGGA CGGCCTGGTC 
AACTTCTCCA AGATGAGAAT GATCTCCCAT ATTATCCGAG AGATTCGCCA ATTTCAGCAG 
ACGACCTACA AAATCGAGCC CCAGCCAAAG GTAATTCAGT ACTTACTGGA TGAATCTTTC 
ATGTTGGACG AAGAGAGTCT GTATGAGTCT TCGCTCCTAA TTGAACCAAA ACTCCCCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021062598.1
CDS	401..4183
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQQHYFTVNFSNDSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKTEITNLIKDNDRVQSSNKAASADDEDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLMQVSLSEKSKSSRGRLGSLSIKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLDEPENLDDEAKGAGPEIEHLEFKIGVEPKDSPPFTVILVASSRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAMVVLDKLINIYRKPMSAIPARSLELLFSSSHNAKLLYGDAPKSPRASRKFSSPPPLAIGTSSPSRRRKLSLNIPIITGGKALELASLGCSSDSYANIHSPISPFGKTTLDTGKLCMASSLPKTPEEIDVPTTIPEKPGELSASRKQSSDILKGESDGDQNHSDEDNTEVSPVKSPPTPKSFLNRTITEFPFFNYNNGVLMTTCRDLVDNNRSTLSATSAFAIATAGANEGPSNKEVFRRMSLANTGFSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFLEEVMHDPDLLTQERKAAANIIRTLTLEETTEQHSMLEEVILMTEGVKTEPFESHPALEIAEQLTLLDHLVFKSIPYEEFFGQGWMKMEKYERTPYIMKTTKHFNHVSNFIASEIIRNEDINARASAIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLLDKLQKLVSSDGRFKNLREALRNCDPPCVPYLGMYLTDLVFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIEPQPKVIQYLLDESFMLDEESLYESSLLIEPKLPT

Target ORF information:

RefSeq Version	XM_021206939.2
Organism	Mus pahari(shrew mouse)
Definition	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021206939.2

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ACTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGCTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCCG CCATCGCTCG TGCCAGCTAC AAGATACTGG CTACAGAGCA CGAGGCGCTC 
ATGCAGAAGT ACTTGCACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAACAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGATTACA 
AACCTGATCA AGGACAATGA CCGTGTTCAA TCCAGCAACA AGGCCGCTTC TGCTGATGAT 
GAGGACAGTG ACATCAAGAA AATTAAGAAG GTTCAGAGCT TCCTTCGGGG ATGGCTGTGC 
CGTCGAAAGT GGAAGAACAT CATCCAGGAC TACATCCGGT CTCCCCACGC GGACAGCATG 
CGCAAGAGGA ACCAGGTGGT ATTCAGCATG CTGGAGGCGG AGGCTGAGTA CGTGCAGCAG 
CTACACATCC TTGTCAACAA CTTCCTGCGC CCACTGCGCA TGGCCGCCAG CTCTAAGAAA 
CCCCCTATAA CACACGACGA CGTCAGCAGT ATCTTTCTGA ACAGCGAGAC CATCATGTTC 
CTGCATCAGA TCTTCTACCA AGGCCTGAAG GCCCGTATCT CTAGCTGGCC CACCCTGGTG 
CTGGCGGACC TGTTCGACAT CCTGCTGCCC ATGCTGAACA TCTACCAGGA GTTCGTCCGC 
AACCACCAGT ACAGCCTCCA GATCCTTGCT CACTGCAAGC AAAACCGGGA CTTTGACAAG 
CTCCTCAAGC AGTATGAGGC CAAGCCAGAC TGCGAGGAGC GCACACTGGA GACCTTCCTC 
ACCTACCCAA TGTTCCAGAT CCCCAGGTAC ATCCTGACAC TCCATGAGCT GCTGGCCCAC 
ACACCCCATG AGCATGTGGA GCGTAACAGC CTGGACTATG CCAAATCCAA ACTAGAGGAA 
CTGTCCAGGA TCATGCATGA CGAAGTCAGT GAGACCGAAA ACATCCGCAA AAACCTGGCC 
ATTGAGCGTA TGATCACTGA GGGCTGTGAG ATCCTCCTGG ACACCAGCCA GACCTTCGTG 
CGCCAAGGTT CCCTCATGCA GGTGTCCTTG TCAGAAAAGA GCAAGTCCTC TAGGGGCCGC 
CTGGGGTCTC TGTCCATTAA GAAAGAGGGT GAGCGCCAGT GTTTCCTGTT CTCCAAGCAT 
CTCATCATCT GCACCAGAGG CTCTGGTGGG AAACTGCACC TAACCAAGAA TGGAGTCATA 
TCCCTCATTG ACTGCACCTT ACTGGATGAA CCCGAAAACC TGGACGACGA AGCCAAAGGA 
GCTGGACCAG AGATAGAGCA CCTGGAGTTT AAGATCGGGG TGGAGCCAAA GGACTCCCCG 
CCCTTCACCG TCATCCTGGT GGCCTCATCC AGGCAGGAGA AGGCAGCATG GACCAGTGAC 
ATCATCCAGT GCGTGGATAA TATCCGCTGC AATGGACTCA TGATGAATGC CTTCGAAGAA 
AATTCCAAGG TCACCGTGCC TCAGATGATC AAGTCTGACG CTTCCTTATA TTGTGATGAT 
GTTGACATTC GCTTCAGCAA GACCATGAAT TCTTGCAAAG TGCTTCAGAT CCGCTATGCC 
AGCGTGGAGC GCCTGCTGGA GCGCCTGACC GATCTTCGCT TCCTGAGCAT TGACTTTCTC 
AACACCTTCC TGCATTCCTA TCGAGTCTTC ACCACTGCTA TGGTGGTCCT GGACAAGCTA 
ATCAACATCT ACAGAAAACC CATGAGTGCC ATTCCTGCCA GGTCCCTGGA ACTCTTGTTT 
TCCAGCAGCC ACAACGCCAA GCTTCTGTAC GGAGATGCCC CCAAATCCCC TCGCGCCAGC 
CGCAAGTTCT CCTCGCCGCC GCCCTTGGCC ATCGGGACAT CGTCCCCGAG CCGCCGTCGG 
AAGCTGTCCC TCAACATCCC CATCATCACA GGTGGCAAGG CACTGGAACT GGCTTCACTT 
GGGTGCAGCT CCGATAGCTA CGCCAACATA CACTCACCCA TATCCCCCTT CGGCAAAACC 
ACACTGGACA CTGGCAAGCT CTGTATGGCC AGCAGCCTAC CCAAGACTCC AGAGGAGATT 
GACGTGCCTA CCACAATTCC AGAAAAGCCT GGGGAGCTCT CAGCCTCCAG AAAGCAGAGC 
TCTGACATCC TGAAAGGAGA GTCTGATGGC GACCAGAACC ACAGTGATGA AGACAACACA 
GAAGTGTCGC CAGTTAAATC TCCACCAACA CCGAAGTCGT TCTTAAACAG AACCATCACA 
GAGTTCCCAT TCTTTAACTA CAACAACGGA GTCCTGATGA CCACGTGTCG CGATCTGGTG 
GACAATAACC GCAGCACTCT CTCAGCTACT TCTGCCTTTG CCATAGCGAC TGCAGGAGCC 
AATGAAGGCC CCTCAAACAA GGAGGTCTTT CGAAGGATGT CTTTGGCCAA CACAGGCTTT 
TCCTCTGACC AGAGAAATAT CGACAAGGAG TTTGTGATCC GCAGAGCTGC CACCAATCGA 
GTGTTGAATG TGCTCCGCCA CTGGGTCACC AAGCACACCC AGGACTTTGA TACCGACGAT 
ACGCTCAAAT ACAGGGTGAT CTGCTTCTTG GAGGAGGTCA TGCATGACCC AGACCTTCTG 
ACCCAAGAGC GAAAGGCAGC AGCCAACATC ATCAGGACTC TGACCCTAGA AGAAACAACT 
GAACAACATA GCATGCTGGA AGAAGTCATA CTGATGACGG AGGGTGTGAA GACTGAGCCC 
TTCGAAAGCC ACCCAGCCCT GGAGATAGCA GAGCAGCTGA CCCTACTGGA TCACCTTGTC 
TTCAAGAGTA TTCCTTATGA AGAGTTCTTT GGCCAGGGCT GGATGAAGAT GGAGAAGTAT 
GAAAGGACAC CTTACATTAT GAAAACCACC AAACACTTCA ACCATGTCAG CAACTTCATT 
GCTTCAGAAA TTATCCGAAA CGAGGACATC AACGCAAGGG CAAGCGCCAT CGAGAAGTGG 
GTGGCCGTGG CCGACATATG TCGCTGCCTG CACAACTACA ATGCTGTGCT GGAGATCACC 
TCCTCCATCA ACCGCAGCGC CATCTTCCGA CTCAAAAAGA CATGGCTCAA AGTTTCTAAG 
CAGACGAAAT CTTTGCTTGA CAAGCTCCAA AAGCTTGTGT CATCAGATGG CAGATTTAAG 
AACCTCAGAG AGGCTTTGAG AAATTGCGAT CCACCTTGTG TCCCTTACCT GGGGATGTAT 
CTCACCGACT TGGTGTTCAT CGAGGAGGGG ACACCCAATT ACACAGAGGA CGGCCTGGTC 
AACTTCTCCA AGATGAGAAT GATCTCCCAT ATTATCCGAG AGATTCGCCA ATTTCAGCAG 
ACGACCTACA AAATCGAGCC CCAGCCAAAG GTAATTCAGT ACTTACTGGA TGAATCTTTC 
ATGTTGGACG AAGAGAGTCT GTATGAGTCT TCGCTCCTAA TTGAACCAAA ACTCCCCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf1 ( XM_021206940.1 ) cDNA ORF clone, Mus pahari(shrew mouse) -> XP_021062599.1 Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X3, mRNA.

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CloneID	OMv47901
Clone ID Related Accession (Same CDS sequence)	XM_021206940.1
Accession Version	XM_021206940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-18
Organism	Mus pahari(shrew mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034599.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus pahari Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ACTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGCTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCCG CCATCGCTCG TGCCAGCTAC AAGATACTGG CTACAGAGCA CGAGGCGCTC 
ATGCAGAAGT ACTTGCACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAACAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGATTACA 
AACCTGATCA AGGACAATGA CCGTGTTCAA TCCAGCAACA AGGCCGCTTC TGCTGATGAT 
GAGGACAGTG ACATCAAGAA AATTAAGAAG GTTCAGAGCT TCCTTCGGGG ATGGCTGTGC 
CGTCGAAAGT GGAAGAACAT CATCCAGGAC TACATCCGGT CTCCCCACGC GGACAGCATG 
CGCAAGAGGA ACCAGGTGGT ATTCAGCATG CTGGAGGCGG AGGCTGAGTA CGTGCAGCAG 
CTACACATCC TTGTCAACAA CTTCCTGCGC CCACTGCGCA TGGCCGCCAG CTCTAAGAAA 
CCCCCTATAA CACACGACGA CGTCAGCAGT ATCTTTCTGA ACAGCGAGAC CATCATGTTC 
CTGCATCAGA TCTTCTACCA AGGCCTGAAG GCCCGTATCT CTAGCTGGCC CACCCTGGTG 
CTGGCGGACC TGTTCGACAT CCTGCTGCCC ATGCTGAACA TCTACCAGGA GTTCGTCCGC 
AACCACCAGT ACAGCCTCCA GATCCTTGCT CACTGCAAGC AAAACCGGGA CTTTGACAAG 
CTCCTCAAGC AGTATGAGGC CAAGCCAGAC TGCGAGGAGC GCACACTGGA GACCTTCCTC 
ACCTACCCAA TGTTCCAGAT CCCCAGGTAC ATCCTGACAC TCCATGAGCT GCTGGCCCAC 
ACACCCCATG AGCATGTGGA GCGTAACAGC CTGGACTATG CCAAATCCAA ACTAGAGGAA 
CTGTCCAGGA TCATGCATGA CGAAGTCAGT GAGACCGAAA ACATCCGCAA AAACCTGGCC 
ATTGAGCGTA TGATCACTGA GGGCTGTGAG ATCCTCCTGG ACACCAGCCA GACCTTCGTG 
CGCCAAGGTT CCCTCATGCA GGTGTCCTTG TCAGAAAAGA GCAAGTCCTC TAGGGGCCGC 
CTGGGGTCTC TGTCCATTAA GAAAGAGGGT GAGCGCCAGT GTTTCCTGTT CTCCAAGCAT 
CTCATCATCT GCACCAGAGG CTCTGGTGGG AAACTGCACC TAACCAAGAA TGGAGTCATA 
TCCCTCATTG ACTGCACCTT ACTGGATGAA CCCGAAAACC TGGACGACGA AGGAGCTGGA 
CCAGAGATAG AGCACCTGGA GTTTAAGATC GGGGTGGAGC CAAAGGACTC CCCGCCCTTC 
ACCGTCATCC TGGTGGCCTC ATCCAGGCAG GAGAAGGCAG CATGGACCAG TGACATCATC 
CAGTGCGTGG ATAATATCCG CTGCAATGGA CTCATGATGA ATGCCTTCGA AGAAAATTCC 
AAGGTCACCG TGCCTCAGAT GATCAAGTCT GACGCTTCCT TATATTGTGA TGATGTTGAC 
ATTCGCTTCA GCAAGACCAT GAATTCTTGC AAAGTGCTTC AGATCCGCTA TGCCAGCGTG 
GAGCGCCTGC TGGAGCGCCT GACCGATCTT CGCTTCCTGA GCATTGACTT TCTCAACACC 
TTCCTGCATT CCTATCGAGT CTTCACCACT GCTATGGTGG TCCTGGACAA GCTAATCAAC 
ATCTACAGAA AACCCATGAG TGCCATTCCT GCCAGGTCCC TGGAACTCTT GTTTTCCAGC 
AGCCACAACG CCAAGCTTCT GTACGGAGAT GCCCCCAAAT CCCCTCGCGC CAGCCGCAAG 
TTCTCCTCGC CGCCGCCCTT GGCCATCGGG ACATCGTCCC CGAGCCGCCG TCGGAAGCTG 
TCCCTCAACA TCCCCATCAT CACAGGTGGC AAGGCACTGG AACTGGCTTC ACTTGGGTGC 
AGCTCCGATA GCTACGCCAA CATACACTCA CCCATATCCC CCTTCGGCAA AACCACACTG 
GACACTGGCA AGCTCTGTAT GGCCAGCAGC CTACCCAAGA CTCCAGAGGA GATTGACGTG 
CCTACCACAA TTCCAGAAAA GCCTGGGGAG CTCTCAGCCT CCAGAAAGCA GAGCTCTGAC 
ATCCTGAAAG GAGAGTCTGA TGGCGACCAG AACCACAGTG ATGAAGACAA CACAGAAGTG 
TCGCCAGTTA AATCTCCACC AACACCGAAG TCGTTCTTAA ACAGAACCAT CACAGAGTTC 
CCATTCTTTA ACTACAACAA CGGAGTCCTG ATGACCACGT GTCGCGATCT GGTGGACAAT 
AACCGCAGCA CTCTCTCAGC TACTTCTGCC TTTGCCATAG CGACTGCAGG AGCCAATGAA 
GGCCCCTCAA ACAAGGAGGT CTTTCGAAGG ATGTCTTTGG CCAACACAGG CTTTTCCTCT 
GACCAGAGAA ATATCGACAA GGAGTTTGTG ATCCGCAGAG CTGCCACCAA TCGAGTGTTG 
AATGTGCTCC GCCACTGGGT CACCAAGCAC ACCCAGGACT TTGATACCGA CGATACGCTC 
AAATACAGGG TGATCTGCTT CTTGGAGGAG GTCATGCATG ACCCAGACCT TCTGACCCAA 
GAGCGAAAGG CAGCAGCCAA CATCATCAGG ACTCTGACCC TAGAAGAAAC AACTGAACAA 
CATAGCATGC TGGAAGAAGT CATACTGATG ACGGAGGGTG TGAAGACTGA GCCCTTCGAA 
AGCCACCCAG CCCTGGAGAT AGCAGAGCAG CTGACCCTAC TGGATCACCT TGTCTTCAAG 
AGTATTCCTT ATGAAGAGTT CTTTGGCCAG GGCTGGATGA AGATGGAGAA GTATGAAAGG 
ACACCTTACA TTATGAAAAC CACCAAACAC TTCAACCATG TCAGCAACTT CATTGCTTCA 
GAAATTATCC GAAACGAGGA CATCAACGCA AGGGCAAGCG CCATCGAGAA GTGGGTGGCC 
GTGGCCGACA TATGTCGCTG CCTGCACAAC TACAATGCTG TGCTGGAGAT CACCTCCTCC 
ATCAACCGCA GCGCCATCTT CCGACTCAAA AAGACATGGC TCAAAGTTTC TAAGCAGACG 
AAATCTTTGC TTGACAAGCT CCAAAAGCTT GTGTCATCAG ATGGCAGATT TAAGAACCTC 
AGAGAGGCTT TGAGAAATTG CGATCCACCT TGTGTCCCTT ACCTGGGGAT GTATCTCACC 
GACTTGGTGT TCATCGAGGA GGGGACACCC AATTACACAG AGGACGGCCT GGTCAACTTC 
TCCAAGATGA GAATGATCTC CCATATTATC CGAGAGATTC GCCAATTTCA GCAGACGACC 
TACAAAATCG AGCCCCAGCC AAAGGTAATT CAGTACTTAC TGGATGAATC TTTCATGTTG 
GACGAAGAGA GTCTGTATGA GTCTTCGCTC CTAATTGAAC CAAAACTCCC CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021062599.1
CDS	1..3777
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQQHYFTVNFSNDSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKTEITNLIKDNDRVQSSNKAASADDEDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLMQVSLSEKSKSSRGRLGSLSIKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLDEPENLDDEGAGPEIEHLEFKIGVEPKDSPPFTVILVASSRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAMVVLDKLINIYRKPMSAIPARSLELLFSSSHNAKLLYGDAPKSPRASRKFSSPPPLAIGTSSPSRRRKLSLNIPIITGGKALELASLGCSSDSYANIHSPISPFGKTTLDTGKLCMASSLPKTPEEIDVPTTIPEKPGELSASRKQSSDILKGESDGDQNHSDEDNTEVSPVKSPPTPKSFLNRTITEFPFFNYNNGVLMTTCRDLVDNNRSTLSATSAFAIATAGANEGPSNKEVFRRMSLANTGFSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFLEEVMHDPDLLTQERKAAANIIRTLTLEETTEQHSMLEEVILMTEGVKTEPFESHPALEIAEQLTLLDHLVFKSIPYEEFFGQGWMKMEKYERTPYIMKTTKHFNHVSNFIASEIIRNEDINARASAIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLLDKLQKLVSSDGRFKNLREALRNCDPPCVPYLGMYLTDLVFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIEPQPKVIQYLLDESFMLDEESLYESSLLIEPKLPT

Target ORF information:

RefSeq Version	XM_021206940.1
Organism	Mus pahari(shrew mouse)
Definition	Mus pahari Ras protein specific guanine nucleotide releasing factor 1 (Rasgrf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021206940.1

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT CAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGCG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTCTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCCG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
AGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ACTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGCTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCCG CCATCGCTCG TGCCAGCTAC AAGATACTGG CTACAGAGCA CGAGGCGCTC 
ATGCAGAAGT ACTTGCACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAACAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGATTACA 
AACCTGATCA AGGACAATGA CCGTGTTCAA TCCAGCAACA AGGCCGCTTC TGCTGATGAT 
GAGGACAGTG ACATCAAGAA AATTAAGAAG GTTCAGAGCT TCCTTCGGGG ATGGCTGTGC 
CGTCGAAAGT GGAAGAACAT CATCCAGGAC TACATCCGGT CTCCCCACGC GGACAGCATG 
CGCAAGAGGA ACCAGGTGGT ATTCAGCATG CTGGAGGCGG AGGCTGAGTA CGTGCAGCAG 
CTACACATCC TTGTCAACAA CTTCCTGCGC CCACTGCGCA TGGCCGCCAG CTCTAAGAAA 
CCCCCTATAA CACACGACGA CGTCAGCAGT ATCTTTCTGA ACAGCGAGAC CATCATGTTC 
CTGCATCAGA TCTTCTACCA AGGCCTGAAG GCCCGTATCT CTAGCTGGCC CACCCTGGTG 
CTGGCGGACC TGTTCGACAT CCTGCTGCCC ATGCTGAACA TCTACCAGGA GTTCGTCCGC 
AACCACCAGT ACAGCCTCCA GATCCTTGCT CACTGCAAGC AAAACCGGGA CTTTGACAAG 
CTCCTCAAGC AGTATGAGGC CAAGCCAGAC TGCGAGGAGC GCACACTGGA GACCTTCCTC 
ACCTACCCAA TGTTCCAGAT CCCCAGGTAC ATCCTGACAC TCCATGAGCT GCTGGCCCAC 
ACACCCCATG AGCATGTGGA GCGTAACAGC CTGGACTATG CCAAATCCAA ACTAGAGGAA 
CTGTCCAGGA TCATGCATGA CGAAGTCAGT GAGACCGAAA ACATCCGCAA AAACCTGGCC 
ATTGAGCGTA TGATCACTGA GGGCTGTGAG ATCCTCCTGG ACACCAGCCA GACCTTCGTG 
CGCCAAGGTT CCCTCATGCA GGTGTCCTTG TCAGAAAAGA GCAAGTCCTC TAGGGGCCGC 
CTGGGGTCTC TGTCCATTAA GAAAGAGGGT GAGCGCCAGT GTTTCCTGTT CTCCAAGCAT 
CTCATCATCT GCACCAGAGG CTCTGGTGGG AAACTGCACC TAACCAAGAA TGGAGTCATA 
TCCCTCATTG ACTGCACCTT ACTGGATGAA CCCGAAAACC TGGACGACGA AGGAGCTGGA 
CCAGAGATAG AGCACCTGGA GTTTAAGATC GGGGTGGAGC CAAAGGACTC CCCGCCCTTC 
ACCGTCATCC TGGTGGCCTC ATCCAGGCAG GAGAAGGCAG CATGGACCAG TGACATCATC 
CAGTGCGTGG ATAATATCCG CTGCAATGGA CTCATGATGA ATGCCTTCGA AGAAAATTCC 
AAGGTCACCG TGCCTCAGAT GATCAAGTCT GACGCTTCCT TATATTGTGA TGATGTTGAC 
ATTCGCTTCA GCAAGACCAT GAATTCTTGC AAAGTGCTTC AGATCCGCTA TGCCAGCGTG 
GAGCGCCTGC TGGAGCGCCT GACCGATCTT CGCTTCCTGA GCATTGACTT TCTCAACACC 
TTCCTGCATT CCTATCGAGT CTTCACCACT GCTATGGTGG TCCTGGACAA GCTAATCAAC 
ATCTACAGAA AACCCATGAG TGCCATTCCT GCCAGGTCCC TGGAACTCTT GTTTTCCAGC 
AGCCACAACG CCAAGCTTCT GTACGGAGAT GCCCCCAAAT CCCCTCGCGC CAGCCGCAAG 
TTCTCCTCGC CGCCGCCCTT GGCCATCGGG ACATCGTCCC CGAGCCGCCG TCGGAAGCTG 
TCCCTCAACA TCCCCATCAT CACAGGTGGC AAGGCACTGG AACTGGCTTC ACTTGGGTGC 
AGCTCCGATA GCTACGCCAA CATACACTCA CCCATATCCC CCTTCGGCAA AACCACACTG 
GACACTGGCA AGCTCTGTAT GGCCAGCAGC CTACCCAAGA CTCCAGAGGA GATTGACGTG 
CCTACCACAA TTCCAGAAAA GCCTGGGGAG CTCTCAGCCT CCAGAAAGCA GAGCTCTGAC 
ATCCTGAAAG GAGAGTCTGA TGGCGACCAG AACCACAGTG ATGAAGACAA CACAGAAGTG 
TCGCCAGTTA AATCTCCACC AACACCGAAG TCGTTCTTAA ACAGAACCAT CACAGAGTTC 
CCATTCTTTA ACTACAACAA CGGAGTCCTG ATGACCACGT GTCGCGATCT GGTGGACAAT 
AACCGCAGCA CTCTCTCAGC TACTTCTGCC TTTGCCATAG CGACTGCAGG AGCCAATGAA 
GGCCCCTCAA ACAAGGAGGT CTTTCGAAGG ATGTCTTTGG CCAACACAGG CTTTTCCTCT 
GACCAGAGAA ATATCGACAA GGAGTTTGTG ATCCGCAGAG CTGCCACCAA TCGAGTGTTG 
AATGTGCTCC GCCACTGGGT CACCAAGCAC ACCCAGGACT TTGATACCGA CGATACGCTC 
AAATACAGGG TGATCTGCTT CTTGGAGGAG GTCATGCATG ACCCAGACCT TCTGACCCAA 
GAGCGAAAGG CAGCAGCCAA CATCATCAGG ACTCTGACCC TAGAAGAAAC AACTGAACAA 
CATAGCATGC TGGAAGAAGT CATACTGATG ACGGAGGGTG TGAAGACTGA GCCCTTCGAA 
AGCCACCCAG CCCTGGAGAT AGCAGAGCAG CTGACCCTAC TGGATCACCT TGTCTTCAAG 
AGTATTCCTT ATGAAGAGTT CTTTGGCCAG GGCTGGATGA AGATGGAGAA GTATGAAAGG 
ACACCTTACA TTATGAAAAC CACCAAACAC TTCAACCATG TCAGCAACTT CATTGCTTCA 
GAAATTATCC GAAACGAGGA CATCAACGCA AGGGCAAGCG CCATCGAGAA GTGGGTGGCC 
GTGGCCGACA TATGTCGCTG CCTGCACAAC TACAATGCTG TGCTGGAGAT CACCTCCTCC 
ATCAACCGCA GCGCCATCTT CCGACTCAAA AAGACATGGC TCAAAGTTTC TAAGCAGACG 
AAATCTTTGC TTGACAAGCT CCAAAAGCTT GTGTCATCAG ATGGCAGATT TAAGAACCTC 
AGAGAGGCTT TGAGAAATTG CGATCCACCT TGTGTCCCTT ACCTGGGGAT GTATCTCACC 
GACTTGGTGT TCATCGAGGA GGGGACACCC AATTACACAG AGGACGGCCT GGTCAACTTC 
TCCAAGATGA GAATGATCTC CCATATTATC CGAGAGATTC GCCAATTTCA GCAGACGACC 
TACAAAATCG AGCCCCAGCC AAAGGTAATT CAGTACTTAC TGGATGAATC TTTCATGTTG 
GACGAAGAGA GTCTGTATGA GTCTTCGCTC CTAATTGAAC CAAAACTCCC CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Rasgrf1 cDNA ORF clone, Mus pahari(shrew mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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