Aplf cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aplf
Entrez Gene ID	72103
Full Name	aprataxin and PNKP like factor
Synonyms	2010301N04Rik,AI452191
General protein information	Preferred Names aprataxin and PNKP like factor Names aprataxin and PNK-like factor apurinic-apyrimidinic endonuclease APLF
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 D1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006506657.3	XP_006506720.1	aprataxin and PNK-like factor isoform X2
XM_006506657.4	XP_006506720.1	aprataxin and PNK-like factor isoform X2
XM_006506656.3	XP_006506719.1	aprataxin and PNK-like factor isoform X2
XM_006506656.4	XP_006506719.1	aprataxin and PNK-like factor isoform X2
NM_024251.4	NP_077213.2	aprataxin and PNK-like factor isoform 2
XM_017321755.1	XP_017177244.1	aprataxin and PNK-like factor isoform X1
NM_001372332.1	NP_001359261.1	aprataxin and PNK-like factor isoform 3
NM_001170489.1	NP_001163960.1	aprataxin and PNK-like factor isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	APLF	XP_538525.2
Bos taurus (cattle)	APLF	NP_001071342.1
Homo sapiens (human)	APLF	NP_775816.1
Rattus norvegicus (Norway rat)	Aplf	NP_001166853.1
Gallus gallus (chicken)	APLF	XP_419335.3
Xenopus tropicalis (tropical clawed frog)	aplf	NP_001106602.1
Mus musculus (house mouse)	Aplf	NP_001163960.1
	APLF	XP_002799328.1

Gene Ontology
Bibliography

Related articles in PubMed

Histone chaperone APLF regulates induction of pluripotency in murine fibroblasts.
Syed KM, Joseph S, Mukherjee A, Majumder A, Teixeira JM, Dutta D, Pillai MR
Journal of cell science129(24)4576-4591(2016 12)

PARP-3 and APLF function together to accelerate nonhomologous end-joining.
Rulten SL, Fisher AE, Robert I, Zuma MC, Rouleau M, Ju L, Poirier G, Reina-San-Martin B, Caldecott KW
Molecular cell41(1)33-45(2011 Jan)

Quantitative genetics of age-related retinal degeneration: a second F1 intercross between the A/J and C57BL/6 strains.
Danciger M, Yang H, Ralston R, Liu Y, Matthes MT, Peirce J, Lavail MM
Molecular vision1379-85(2007 Jan)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the downregulation of histone chaperone Aprataxin PNK-like factor (APLF) promotes reprogramming by augmenting the expression of E-cadherin.
Title: Histone chaperone APLF regulates induction of pluripotency in murine fibroblasts.

APLF has a role in chromosomal DNA double-strand break repair.
Title: PARP-3 and APLF function together to accelerate nonhomologous end-joining.

The following Aplf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aplf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05122	NM_024251.4 Latest version!	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu11601	NM_001170489.1 Latest version!	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu81803	NM_001372332.1 Latest version!	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu37609	XM_006506657.4 Latest version!	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu37609	XM_006506656.4 Latest version!	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu81803	XM_017321755.1 Latest version!	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu37609	XM_006506657.3	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu37609	XM_006506656.3	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aplf ( NM_024251.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077213.2 Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant 2, mRNA.

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CloneID	OMu05122
Clone ID Related Accession (Same CDS sequence)	NM_024251.4
Accession Version	NM_024251.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	aprataxin and PNK-like factor isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK008513.1, AK034191.1 and AC125171.4. On Dec 19, 2009 this sequence version replaced NM_024251.3. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence and in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has distinct N- and C-termini and is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK034191.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCAGCG TGCCTGAGGG AGGTGGCTAC GAGATAACAG ACAAAAGAGT ATCCAGAAGA 
CATGCCATTC TTGAAGTGGT TGATAGTCAG CTTCGAATCA AGCCAATCCA TAGGAATCCA 
TGTTTCTACC AGTCCTCGGA GAAGAGCCAA CACTCGCCAA TGGAGACGCA AGTGTGGAGC 
CAGTTGCATC CCGGGGATAG CTTTTCCCTG TTACTTGACA AGTACGCTTT CCGTGTTTTC 
TCTGCAGAAT CTGAAGTGGA GATGGAGTGT ACTCTGAGAA ACAGTCAGAT GCTTGATGAA 
GATGATATAT TGAGTGAGAT GCAGAAGTCC CCTGTGGTTA ACTTACCTGA TAAGACAACT 
GGTGCCTCAC AGCTGCAAGG AAGCCCTGAA ATAACCAAGA CCAAGTGTCC CACCATAGAT 
CCTATGTCCT CTTCAGGTGA ATGCAGAGCT TTCAGTGAGC ACCAGCCACG TCCTACCCAG 
AGGAAAAGAA TCCTCCCAGC TTGGATGTTG GCGGAAAGTC TAAGTGACCA GAGCCTCTCC 
ACACCAGCTG AGGGGGGAGA TAAGGATGTC ATCCAGAGGA GTGGAAAAGC AGGAACCTGT 
GAAGATAGAA CCCCAGGAAA CACATCATGG CATGGGAAAA AGCGGCTTAG TCCATCAGGA 
AATTCAAAAA GTGTGTCAGC GGAACAAGAC CCTGGGAAGA AGTGCAGAAA GGCTGACCAA 
GAAGGACCGG GTGTTTCTTC TGAGAATGTT CCAGAATCAT CATCTTCAAA CATAGTAAAG 
GACCCAGATG TGGATATTGT GAAGACAAAT AAACAGAAGG ACGGGATTTT AATAGAGGAA 
CTTGGTGAGG TCTCTAAACA TAAAGCCGCC ACCAAACCAA CCACAAATGA AGAGGGCGAG 
AGTTGTGCAC GTGTCCAAAG CAAGTCACCT CCTGAGAAAT CTCAAGGATG CCATCCTGAG 
TCTAGCTCTG CTCCCTCCAG TCCTGATGCT TTGCACACAG ACACAGCTGA CCCAGTGCTG 
GGCTGTTCAG AAGAAAGCAA GGTCCGGAGG ACGGCCTGCA TGTATGGGGC CAACTGCTAC 
AGGAGGAACC CCCTCCACTT TCAACACTTC AGCCACCCTG GTGACAGTGA CTATGGAGAG 
GTACATGGCA CAGATGAAGG TGTGATTGGT GACAGGCCCG AGTGTCCCTA TGGAGCGTCT 
TGTTACAGGA AGAACCCCCA GCACAAGATG GAATATAGGC ACAGTGCACT TCCAGCCAGA 
GTTGCTCTGG ATGAAGATGA CGATGATGTC GGGCAACCCA GTGACGATGA GGACGAGGAA 
GACTATGAGC CGACAGATGA AGATTCTGAT TGGCACCCAG GAAAGGACGA TGAGGAGCAG 
GAAGATGTGG ATGAGCTTTT GAAAGAAGCA AAAAGGTTTA TGAGAAGGAA AAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077213.2
CDS	147..1583
Translation	MPSVPEGGGYEITDKRVSRRHAILEVVDSQLRIKPIHRNPCFYQSSEKSQHSPMETQVWSQLHPGDSFSLLLDKYAFRVFSAESEVEMECTLRNSQMLDEDDILSEMQKSPVVNLPDKTTGASQLQGSPEITKTKCPTIDPMSSSGECRAFSEHQPRPTQRKRILPAWMLAESLSDQSLSTPAEGGDKDVIQRSGKAGTCEDRTPGNTSWHGKKRLSPSGNSKSVSAEQDPGKKCRKADQEGPGVSSENVPESSSSNIVKDPDVDIVKTNKQKDGILIEELGEVSKHKAATKPTTNEEGESCARVQSKSPPEKSQGCHPESSSAPSSPDALHTDTADPVLGCSEESKVRRTACMYGANCYRRNPLHFQHFSHPGDSDYGEVHGTDEGVIGDRPECPYGASCYRKNPQHKMEYRHSALPARVALDEDDDDVGQPSDDEDEEDYEPTDEDSDWHPGKDDEEQEDVDELLKEAKRFMRRKK

Target ORF information:

RefSeq Version	NM_024251.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024251.4

ORF Insert Sequence:

ATGCCCAGCG TGCCTGAGGG AGGTGGCTAC GAGATAACAG ACAAAAGAGT ATCCAGAAGA 
CATGCCATTC TTGAAGTGGT TGATAGTCAG CTTCGAATCA AGCCAATCCA TAGGAATCCA 
TGTTTCTACC AGTCCTCGGA GAAGAGCCAA CACTCGCCAA TGGAGACGCA AGTGTGGAGC 
CAGTTGCATC CCGGGGATAG CTTTTCCCTG TTACTTGACA AGTACGCTTT CCGTGTTTTC 
TCTGCAGAAT CTGAAGTGGA GATGGAGTGT ACTCTGAGAA ACAGTCAGAT GCTTGATGAA 
GATGATATAT TGAGTGAGAT GCAGAAGTCC CCTGTGGTTA ACTTACCTGA TAAGACAACT 
GGTGCCTCAC AGCTGCAAGG AAGCCCTGAA ATAACCAAGA CCAAGTGTCC CACCATAGAT 
CCTATGTCCT CTTCAGGTGA ATGCAGAGCT TTCAGTGAGC ACCAGCCACG TCCTACCCAG 
AGGAAAAGAA TCCTCCCAGC TTGGATGTTG GCGGAAAGTC TAAGTGACCA GAGCCTCTCC 
ACACCAGCTG AGGGGGGAGA TAAGGATGTC ATCCAGAGGA GTGGAAAAGC AGGAACCTGT 
GAAGATAGAA CCCCAGGAAA CACATCATGG CATGGGAAAA AGCGGCTTAG TCCATCAGGA 
AATTCAAAAA GTGTGTCAGC GGAACAAGAC CCTGGGAAGA AGTGCAGAAA GGCTGACCAA 
GAAGGACCGG GTGTTTCTTC TGAGAATGTT CCAGAATCAT CATCTTCAAA CATAGTAAAG 
GACCCAGATG TGGATATTGT GAAGACAAAT AAACAGAAGG ACGGGATTTT AATAGAGGAA 
CTTGGTGAGG TCTCTAAACA TAAAGCCGCC ACCAAACCAA CCACAAATGA AGAGGGCGAG 
AGTTGTGCAC GTGTCCAAAG CAAGTCACCT CCTGAGAAAT CTCAAGGATG CCATCCTGAG 
TCTAGCTCTG CTCCCTCCAG TCCTGATGCT TTGCACACAG ACACAGCTGA CCCAGTGCTG 
GGCTGTTCAG AAGAAAGCAA GGTCCGGAGG ACGGCCTGCA TGTATGGGGC CAACTGCTAC 
AGGAGGAACC CCCTCCACTT TCAACACTTC AGCCACCCTG GTGACAGTGA CTATGGAGAG 
GTACATGGCA CAGATGAAGG TGTGATTGGT GACAGGCCCG AGTGTCCCTA TGGAGCGTCT 
TGTTACAGGA AGAACCCCCA GCACAAGATG GAATATAGGC ACAGTGCACT TCCAGCCAGA 
GTTGCTCTGG ATGAAGATGA CGATGATGTC GGGCAACCCA GTGACGATGA GGACGAGGAA 
GACTATGAGC CGACAGATGA AGATTCTGAT TGGCACCCAG GAAAGGACGA TGAGGAGCAG 
GAAGATGTGG ATGAGCTTTT GAAAGAAGCA AAAAGGTTTA TGAGAAGGAA AAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aplf ( NM_001170489.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001163960.1 Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11601
Clone ID Related Accession (Same CDS sequence)	NM_001170489.1
Accession Version	NM_001170489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	aprataxin and PNK-like factor isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK008513.1 and AC125171.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK008513.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTTCGG ACTTCTTTCT GCAGCCTCTG GACGGCGGTC CCCGCGTGCC CGTGGGGCCG 
GGGCAGACTG TGATAGGCCG CGGGCCGCTG CTGGGAATAA CAGACAAAAG AGTATCCAGA 
AGACATGCCA TTCTTGAAGT GGTTGATAGT CAGCTTCGAA TCAAGCCAAT CCATAGGAAT 
CCATGTTTCT ACCAGTCCTC GGAGAAGAGC CAACACTCGC CAATGGAGAC GCAAGTGTGG 
AGCCAGTTGC ATCCCGGGGA TAGCTTTTCC CTGTTACTTG ACAAGTACGC TTTCCGTGTT 
TTCTCTGCAG AATCTGAAGT GGAGATGGAG TGTACTCTGA GAAACAGTCA GATGCTTGAT 
GAAGATGATA TATTGAGTGA GATGCAGAAG TCCCCTGTGG TTAACTTACC TGATAAGACA 
ACTGGTGCCT CACAGCTGCA AGGAAGCCCT GAAATAACCA AGACCAAGTG TCCCACCATA 
GATCCTATGT CCTCTTCAGG TGAATGCAGA GCTTTCAGTG AGCACCAGCC ACGTCCTACC 
CAGAGGAAAA GAATCCTCCC AGCTTGGATG TTGGCGGAAA GTCTAAGTGA CCAGAGCCTC 
TCCACACCAG CTGAGGGGGG AGATAAGGAT GTCATCCAGA GGAGTGGAAA AGCAGGAACC 
TGTGAAGATA GAACCCCAGG AAACACATCA TGGCATGGGA AAAAGCGGCT TAGTCCATCA 
GGAAATTCAA AAAGTGTGTC AGCGGAACAA GACCCTGGGA AGAAGTGCAG AAAGGCTGAC 
CAAGAAGGAC CGGGTGTTTC TTCTGAGAAT GTTCCAGAAT CATCATCTTC AAACATAGTA 
AAGGACCCAG ATGTGGATAT TGTGAAGACA AATAAACAGA AGGACGGGAT TTTAATAGAG 
GAACTTGGTG AGGTCTCTAA ACATAAAGCC GCCACCAAAC CAACCACAAA TGAAGAGGGC 
GAGAGTTGTG CACGTGTCCA AAGCAAGTCA CCTCCTGAGA AATCTCAAGG ATGCCATCCT 
GAGTCTAGCT CTGCTCCCTC CAGTCCTGAT GCTTTGCACA CAGACACAGC TGACCCAGTG 
CTGGGCTGTT CAGAAGAAAG CAAGGTCCGG AGGACGGCCT GCATGTATGG GGCCAACTGC 
TACAGGAGGA ACCCCCTCCA CTTTCAACAC TTCAGCCACC CTGGTGACAG TGACTATGGA 
GAGGTACATG GCACAGATGA AGGTGTGATT GGTGACAGGC CCGAGTGTCC CTATGGAGCG 
TCTTGTTACA GGAAGAACCC CCAGCACAAG ATGGAATATA GGCACAGTGC ACTTCCAGCC 
AGAGTTGCTC TGGATGAAGA TGACGATGAT GTCGGGCAAC CCAGTGACGA TGAGGACGAG 
GAAGACTATG AGCCGACAGA TGAAGATTCT GATTGGCACC CAGGAAAGGA CGATGAGGAG 
CAGGAAGATG TGGATGAGCT TTTGAAAGAA GCAAAAAGTT CTTTGCACTT GAAACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163960.1
CDS	214..1713
Translation	MPSDFFLQPLDGGPRVPVGPGQTVIGRGPLLGITDKRVSRRHAILEVVDSQLRIKPIHRNPCFYQSSEKSQHSPMETQVWSQLHPGDSFSLLLDKYAFRVFSAESEVEMECTLRNSQMLDEDDILSEMQKSPVVNLPDKTTGASQLQGSPEITKTKCPTIDPMSSSGECRAFSEHQPRPTQRKRILPAWMLAESLSDQSLSTPAEGGDKDVIQRSGKAGTCEDRTPGNTSWHGKKRLSPSGNSKSVSAEQDPGKKCRKADQEGPGVSSENVPESSSSNIVKDPDVDIVKTNKQKDGILIEELGEVSKHKAATKPTTNEEGESCARVQSKSPPEKSQGCHPESSSAPSSPDALHTDTADPVLGCSEESKVRRTACMYGANCYRRNPLHFQHFSHPGDSDYGEVHGTDEGVIGDRPECPYGASCYRKNPQHKMEYRHSALPARVALDEDDDDVGQPSDDEDEEDYEPTDEDSDWHPGKDDEEQEDVDELLKEAKSSLHLKH

Target ORF information:

RefSeq Version	NM_001170489.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170489.1

ORF Insert Sequence:

ATGCCTTCGG ACTTCTTTCT GCAGCCTCTG GACGGCGGTC CCCGCGTGCC CGTGGGGCCG 
GGGCAGACTG TGATAGGCCG CGGGCCGCTG CTGGGAATAA CAGACAAAAG AGTATCCAGA 
AGACATGCCA TTCTTGAAGT GGTTGATAGT CAGCTTCGAA TCAAGCCAAT CCATAGGAAT 
CCATGTTTCT ACCAGTCCTC GGAGAAGAGC CAACACTCGC CAATGGAGAC GCAAGTGTGG 
AGCCAGTTGC ATCCCGGGGA TAGCTTTTCC CTGTTACTTG ACAAGTACGC TTTCCGTGTT 
TTCTCTGCAG AATCTGAAGT GGAGATGGAG TGTACTCTGA GAAACAGTCA GATGCTTGAT 
GAAGATGATA TATTGAGTGA GATGCAGAAG TCCCCTGTGG TTAACTTACC TGATAAGACA 
ACTGGTGCCT CACAGCTGCA AGGAAGCCCT GAAATAACCA AGACCAAGTG TCCCACCATA 
GATCCTATGT CCTCTTCAGG TGAATGCAGA GCTTTCAGTG AGCACCAGCC ACGTCCTACC 
CAGAGGAAAA GAATCCTCCC AGCTTGGATG TTGGCGGAAA GTCTAAGTGA CCAGAGCCTC 
TCCACACCAG CTGAGGGGGG AGATAAGGAT GTCATCCAGA GGAGTGGAAA AGCAGGAACC 
TGTGAAGATA GAACCCCAGG AAACACATCA TGGCATGGGA AAAAGCGGCT TAGTCCATCA 
GGAAATTCAA AAAGTGTGTC AGCGGAACAA GACCCTGGGA AGAAGTGCAG AAAGGCTGAC 
CAAGAAGGAC CGGGTGTTTC TTCTGAGAAT GTTCCAGAAT CATCATCTTC AAACATAGTA 
AAGGACCCAG ATGTGGATAT TGTGAAGACA AATAAACAGA AGGACGGGAT TTTAATAGAG 
GAACTTGGTG AGGTCTCTAA ACATAAAGCC GCCACCAAAC CAACCACAAA TGAAGAGGGC 
GAGAGTTGTG CACGTGTCCA AAGCAAGTCA CCTCCTGAGA AATCTCAAGG ATGCCATCCT 
GAGTCTAGCT CTGCTCCCTC CAGTCCTGAT GCTTTGCACA CAGACACAGC TGACCCAGTG 
CTGGGCTGTT CAGAAGAAAG CAAGGTCCGG AGGACGGCCT GCATGTATGG GGCCAACTGC 
TACAGGAGGA ACCCCCTCCA CTTTCAACAC TTCAGCCACC CTGGTGACAG TGACTATGGA 
GAGGTACATG GCACAGATGA AGGTGTGATT GGTGACAGGC CCGAGTGTCC CTATGGAGCG 
TCTTGTTACA GGAAGAACCC CCAGCACAAG ATGGAATATA GGCACAGTGC ACTTCCAGCC 
AGAGTTGCTC TGGATGAAGA TGACGATGAT GTCGGGCAAC CCAGTGACGA TGAGGACGAG 
GAAGACTATG AGCCGACAGA TGAAGATTCT GATTGGCACC CAGGAAAGGA CGATGAGGAG 
CAGGAAGATG TGGATGAGCT TTTGAAAGAA GCAAAAAGTT CTTTGCACTT GAAACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aplf ( XM_006506657.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506720.1 Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu37609
Clone ID Related Accession (Same CDS sequence)	XM_006506657.3 , XM_006506657.4 , XM_006506656.3 , XM_006506656.4
Accession Version	XM_006506657.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	aprataxin and PNK-like factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506657.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC AAGTGTGGAG CCAGTTGCAT CCCGGGGATA GCTTTTCCCT GTTACTTGAC 
AAGTACGCTT TCCGTGTTTT CTCTGCAGAA TCTGAAGTGG AGATGGAGTG TACTCTGAGA 
AACAGTCAGA TGCTTGATGA AGATGATATA TTGAGTGAGA TGCAGAAGTC CCCTGTGGTT 
AACTTACCTG ATAAGACAAC TGGTGCCTCA CAGCTGCAAG GAAGCCCTGA AATAACCAAG 
ACCAAGTGTC CCACCATAGA TCCTATGTCC TCTTCAGGTG AATGCAGAGC TTTCAGTGAG 
CACCAGCCAC GTCCTACCCA GAGGAAAAGA ATCCTCCCAG CTTGGATGTT GGCGGAAAGT 
CTAAGTGACC AGAGCCTCTC CACACCAGCT GAGGGGGGAG ATAAGGATGT CATCCAGAGG 
AGTGGAAAAG CAGGAACCTG TGAAGATAGA ACCCCAGGAA ACACATCATG GCATGGGAAA 
AAGCGGCTTA GTCCATCAGG AAATTCAAAA AGTGTGTCAG CGGAACAAGA CCCTGGGAAG 
AAGTGCAGAA AGGCTGACCA AGAAGGACCG GGTGTTTCTT CTGAGAATGT TCCAGAATCA 
TCATCTTCAA ACATAGTAAA GGACCCAGAT GTGGATATTG TGAAGACAAA TAAACAGAAG 
GACGGGATTT TAATAGAGGA ACTTGGTGAG GTCTCTAAAC ATAAAGCCGC CACCAAACCA 
ACCACAAATG AAGAGGGCGA GAGTTGTGCA CGTGTCCAAA GCAAGTCACC TCCTGAGAAA 
TCTCAAGGAT GCCATCCTGA GTCTAGCTCT GCTCCCTCCA GTCCTGATGC TTTGCACACA 
GACACAGCTG ACCCAGTGCT GGGCTGTTCA GAAGAAAGCA AGGTCCGGAG GACGGCCTGC 
ATGTATGGGG CCAACTGCTA CAGGAGGAAC CCCCTCCACT TTCAACACTT CAGCCACCCT 
GGTGACAGTG ACTATGGAGA GGTACATGGC ACAGATGAAG GTGTGATTGG TGACAGGCCC 
GAGTGTCCCT ATGGAGCGTC TTGTTACAGG AAGAACCCCC AGCACAAGAT GGAATATAGG 
CACAGTGCAC TTCCAGCCAG AGTTGCTCTG GATGAAGATG ACGATGATGT CGGGCAACCC 
AGTGACGATG AGGACGAGGA AGACTATGAG CCGACAGATG AAGATTCTGA TTGGCACCCA 
GGAAAGGACG ATGAGGAGCA GGAAGATGTG GATGAGCTTT TGAAAGAAGC AAAAAGGTTT 
ATGAGAAGGA AAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506720.1
CDS	799..2076
Translation	METQVWSQLHPGDSFSLLLDKYAFRVFSAESEVEMECTLRNSQMLDEDDILSEMQKSPVVNLPDKTTGASQLQGSPEITKTKCPTIDPMSSSGECRAFSEHQPRPTQRKRILPAWMLAESLSDQSLSTPAEGGDKDVIQRSGKAGTCEDRTPGNTSWHGKKRLSPSGNSKSVSAEQDPGKKCRKADQEGPGVSSENVPESSSSNIVKDPDVDIVKTNKQKDGILIEELGEVSKHKAATKPTTNEEGESCARVQSKSPPEKSQGCHPESSSAPSSPDALHTDTADPVLGCSEESKVRRTACMYGANCYRRNPLHFQHFSHPGDSDYGEVHGTDEGVIGDRPECPYGASCYRKNPQHKMEYRHSALPARVALDEDDDDVGQPSDDEDEEDYEPTDEDSDWHPGKDDEEQEDVDELLKEAKRFMRRKK

Target ORF information:

RefSeq Version	XM_006506657.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506657.3

ORF Insert Sequence:

ATGGAGACGC AAGTGTGGAG CCAGTTGCAT CCCGGGGATA GCTTTTCCCT GTTACTTGAC 
AAGTACGCTT TCCGTGTTTT CTCTGCAGAA TCTGAAGTGG AGATGGAGTG TACTCTGAGA 
AACAGTCAGA TGCTTGATGA AGATGATATA TTGAGTGAGA TGCAGAAGTC CCCTGTGGTT 
AACTTACCTG ATAAGACAAC TGGTGCCTCA CAGCTGCAAG GAAGCCCTGA AATAACCAAG 
ACCAAGTGTC CCACCATAGA TCCTATGTCC TCTTCAGGTG AATGCAGAGC TTTCAGTGAG 
CACCAGCCAC GTCCTACCCA GAGGAAAAGA ATCCTCCCAG CTTGGATGTT GGCGGAAAGT 
CTAAGTGACC AGAGCCTCTC CACACCAGCT GAGGGGGGAG ATAAGGATGT CATCCAGAGG 
AGTGGAAAAG CAGGAACCTG TGAAGATAGA ACCCCAGGAA ACACATCATG GCATGGGAAA 
AAGCGGCTTA GTCCATCAGG AAATTCAAAA AGTGTGTCAG CGGAACAAGA CCCTGGGAAG 
AAGTGCAGAA AGGCTGACCA AGAAGGACCG GGTGTTTCTT CTGAGAATGT TCCAGAATCA 
TCATCTTCAA ACATAGTAAA GGACCCAGAT GTGGATATTG TGAAGACAAA TAAACAGAAG 
GACGGGATTT TAATAGAGGA ACTTGGTGAG GTCTCTAAAC ATAAAGCCGC CACCAAACCA 
ACCACAAATG AAGAGGGCGA GAGTTGTGCA CGTGTCCAAA GCAAGTCACC TCCTGAGAAA 
TCTCAAGGAT GCCATCCTGA GTCTAGCTCT GCTCCCTCCA GTCCTGATGC TTTGCACACA 
GACACAGCTG ACCCAGTGCT GGGCTGTTCA GAAGAAAGCA AGGTCCGGAG GACGGCCTGC 
ATGTATGGGG CCAACTGCTA CAGGAGGAAC CCCCTCCACT TTCAACACTT CAGCCACCCT 
GGTGACAGTG ACTATGGAGA GGTACATGGC ACAGATGAAG GTGTGATTGG TGACAGGCCC 
GAGTGTCCCT ATGGAGCGTC TTGTTACAGG AAGAACCCCC AGCACAAGAT GGAATATAGG 
CACAGTGCAC TTCCAGCCAG AGTTGCTCTG GATGAAGATG ACGATGATGT CGGGCAACCC 
AGTGACGATG AGGACGAGGA AGACTATGAG CCGACAGATG AAGATTCTGA TTGGCACCCA 
GGAAAGGACG ATGAGGAGCA GGAAGATGTG GATGAGCTTT TGAAAGAAGC AAAAAGGTTT 
ATGAGAAGGA AAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aplf ( XM_006506656.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506719.1 Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X2, mRNA.

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CloneID	OMu37609
Clone ID Related Accession (Same CDS sequence)	XM_006506657.3 , XM_006506657.4 , XM_006506656.3 , XM_006506656.4
Accession Version	XM_006506656.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	aprataxin and PNK-like factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506656.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC AAGTGTGGAG CCAGTTGCAT CCCGGGGATA GCTTTTCCCT GTTACTTGAC 
AAGTACGCTT TCCGTGTTTT CTCTGCAGAA TCTGAAGTGG AGATGGAGTG TACTCTGAGA 
AACAGTCAGA TGCTTGATGA AGATGATATA TTGAGTGAGA TGCAGAAGTC CCCTGTGGTT 
AACTTACCTG ATAAGACAAC TGGTGCCTCA CAGCTGCAAG GAAGCCCTGA AATAACCAAG 
ACCAAGTGTC CCACCATAGA TCCTATGTCC TCTTCAGGTG AATGCAGAGC TTTCAGTGAG 
CACCAGCCAC GTCCTACCCA GAGGAAAAGA ATCCTCCCAG CTTGGATGTT GGCGGAAAGT 
CTAAGTGACC AGAGCCTCTC CACACCAGCT GAGGGGGGAG ATAAGGATGT CATCCAGAGG 
AGTGGAAAAG CAGGAACCTG TGAAGATAGA ACCCCAGGAA ACACATCATG GCATGGGAAA 
AAGCGGCTTA GTCCATCAGG AAATTCAAAA AGTGTGTCAG CGGAACAAGA CCCTGGGAAG 
AAGTGCAGAA AGGCTGACCA AGAAGGACCG GGTGTTTCTT CTGAGAATGT TCCAGAATCA 
TCATCTTCAA ACATAGTAAA GGACCCAGAT GTGGATATTG TGAAGACAAA TAAACAGAAG 
GACGGGATTT TAATAGAGGA ACTTGGTGAG GTCTCTAAAC ATAAAGCCGC CACCAAACCA 
ACCACAAATG AAGAGGGCGA GAGTTGTGCA CGTGTCCAAA GCAAGTCACC TCCTGAGAAA 
TCTCAAGGAT GCCATCCTGA GTCTAGCTCT GCTCCCTCCA GTCCTGATGC TTTGCACACA 
GACACAGCTG ACCCAGTGCT GGGCTGTTCA GAAGAAAGCA AGGTCCGGAG GACGGCCTGC 
ATGTATGGGG CCAACTGCTA CAGGAGGAAC CCCCTCCACT TTCAACACTT CAGCCACCCT 
GGTGACAGTG ACTATGGAGA GGTACATGGC ACAGATGAAG GTGTGATTGG TGACAGGCCC 
GAGTGTCCCT ATGGAGCGTC TTGTTACAGG AAGAACCCCC AGCACAAGAT GGAATATAGG 
CACAGTGCAC TTCCAGCCAG AGTTGCTCTG GATGAAGATG ACGATGATGT CGGGCAACCC 
AGTGACGATG AGGACGAGGA AGACTATGAG CCGACAGATG AAGATTCTGA TTGGCACCCA 
GGAAAGGACG ATGAGGAGCA GGAAGATGTG GATGAGCTTT TGAAAGAAGC AAAAAGGTTT 
ATGAGAAGGA AAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506719.1
CDS	146..1423
Translation	METQVWSQLHPGDSFSLLLDKYAFRVFSAESEVEMECTLRNSQMLDEDDILSEMQKSPVVNLPDKTTGASQLQGSPEITKTKCPTIDPMSSSGECRAFSEHQPRPTQRKRILPAWMLAESLSDQSLSTPAEGGDKDVIQRSGKAGTCEDRTPGNTSWHGKKRLSPSGNSKSVSAEQDPGKKCRKADQEGPGVSSENVPESSSSNIVKDPDVDIVKTNKQKDGILIEELGEVSKHKAATKPTTNEEGESCARVQSKSPPEKSQGCHPESSSAPSSPDALHTDTADPVLGCSEESKVRRTACMYGANCYRRNPLHFQHFSHPGDSDYGEVHGTDEGVIGDRPECPYGASCYRKNPQHKMEYRHSALPARVALDEDDDDVGQPSDDEDEEDYEPTDEDSDWHPGKDDEEQEDVDELLKEAKRFMRRKK

Target ORF information:

RefSeq Version	XM_006506656.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506656.3

ORF Insert Sequence:

ATGGAGACGC AAGTGTGGAG CCAGTTGCAT CCCGGGGATA GCTTTTCCCT GTTACTTGAC 
AAGTACGCTT TCCGTGTTTT CTCTGCAGAA TCTGAAGTGG AGATGGAGTG TACTCTGAGA 
AACAGTCAGA TGCTTGATGA AGATGATATA TTGAGTGAGA TGCAGAAGTC CCCTGTGGTT 
AACTTACCTG ATAAGACAAC TGGTGCCTCA CAGCTGCAAG GAAGCCCTGA AATAACCAAG 
ACCAAGTGTC CCACCATAGA TCCTATGTCC TCTTCAGGTG AATGCAGAGC TTTCAGTGAG 
CACCAGCCAC GTCCTACCCA GAGGAAAAGA ATCCTCCCAG CTTGGATGTT GGCGGAAAGT 
CTAAGTGACC AGAGCCTCTC CACACCAGCT GAGGGGGGAG ATAAGGATGT CATCCAGAGG 
AGTGGAAAAG CAGGAACCTG TGAAGATAGA ACCCCAGGAA ACACATCATG GCATGGGAAA 
AAGCGGCTTA GTCCATCAGG AAATTCAAAA AGTGTGTCAG CGGAACAAGA CCCTGGGAAG 
AAGTGCAGAA AGGCTGACCA AGAAGGACCG GGTGTTTCTT CTGAGAATGT TCCAGAATCA 
TCATCTTCAA ACATAGTAAA GGACCCAGAT GTGGATATTG TGAAGACAAA TAAACAGAAG 
GACGGGATTT TAATAGAGGA ACTTGGTGAG GTCTCTAAAC ATAAAGCCGC CACCAAACCA 
ACCACAAATG AAGAGGGCGA GAGTTGTGCA CGTGTCCAAA GCAAGTCACC TCCTGAGAAA 
TCTCAAGGAT GCCATCCTGA GTCTAGCTCT GCTCCCTCCA GTCCTGATGC TTTGCACACA 
GACACAGCTG ACCCAGTGCT GGGCTGTTCA GAAGAAAGCA AGGTCCGGAG GACGGCCTGC 
ATGTATGGGG CCAACTGCTA CAGGAGGAAC CCCCTCCACT TTCAACACTT CAGCCACCCT 
GGTGACAGTG ACTATGGAGA GGTACATGGC ACAGATGAAG GTGTGATTGG TGACAGGCCC 
GAGTGTCCCT ATGGAGCGTC TTGTTACAGG AAGAACCCCC AGCACAAGAT GGAATATAGG 
CACAGTGCAC TTCCAGCCAG AGTTGCTCTG GATGAAGATG ACGATGATGT CGGGCAACCC 
AGTGACGATG AGGACGAGGA AGACTATGAG CCGACAGATG AAGATTCTGA TTGGCACCCA 
GGAAAGGACG ATGAGGAGCA GGAAGATGTG GATGAGCTTT TGAAAGAAGC AAAAAGGTTT 
ATGAGAAGGA AAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aplf ( NM_001372332.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001359261.1 Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant 3, mRNA.

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CloneID	OMu81803
Clone ID Related Accession (Same CDS sequence)	XM_017321755.1 , NM_001372332.1
Accession Version	NM_001372332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-28
Organism	Mus musculus(house mouse)
Product	aprataxin and PNK-like factor isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158626.6 and AC125171.4. On Aug 29, 2019 this sequence version replaced XM_017321755.2. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.755200.1, SRR7345562.3963312.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCTTCGG ACTTCTTTCT GCAGCCTCTG GACGGCGGTC CCCGCGTGCC CGTGGGGCCG 
GGGCAGACTG TGATAGGCCG CGGGCCGCTG CTGGGAATAA CAGACAAAAG AGTATCCAGA 
AGACATGCCA TTCTTGAAGT GGTTGATAGT CAGCTTCGAA TCAAGCCAAT CCATAGGAAT 
CCATGTTTCT ACCAGTCCTC GGAGAAGAGC CAACACTCGC CAATGGAGAC GCAAGTGTGG 
AGCCAGTTGC ATCCCGGGGA TAGCTTTTCC CTGTTACTTG ACAAGTACGC TTTCCGTGTT 
TTCTCTGCAG AATCTGAAGT GGAGATGGAG TGTACTCTGA GAAACAGTCA GATGCTTGAT 
GAAGATGATA TATTGAGTGA GATGCAGAAG TCCCCTGTGG TTAACTTACC TGATAAGACA 
ACTGGTGCCT CACAGCTGCA AGGAAGCCCT GAAATAACCA AGACCAAGTG TCCCACCATA 
GATCCTATGT CCTCTTCAGG TGAATGCAGA GCTTTCAGTG AGCACCAGCC ACGTCCTACC 
CAGAGGAAAA GAATCCTCCC AGCTTGGATG TTGGCGGAAA GTCTAAGTGA CCAGAGCCTC 
TCCACACCAG CTGAGGGGGG AGATAAGGAT GTCATCCAGA GGAGTGGAAA AGCAGGAACC 
TGTGAAGATA GAACCCCAGG AAACACATCA TGGCATGGGA AAAAGCGGCT TAGTCCATCA 
GGAAATTCAA AAAGTGTGTC AGCGGAACAA GACCCTGGGA AGAAGTGCAG AAAGGCTGAC 
CAAGAAGGAC CGGGTGTTTC TTCTGAGAAT GTTCCAGAAT CATCATCTTC AAACATAGTA 
AAGGACCCAG ATGTGGATAT TGTGAAGACA AATAAACAGA AGGACGGGAT TTTAATAGAG 
GAACTTGGTG AGGTCTCTAA ACATAAAGCC GCCACCAAAC CAACCACAAA TGAAGAGGGC 
GAGAGTTGTG CACGTGTCCA AAGCAAGTCA CCTCCTGAGA AATCTCAAGG ATGCCATCCT 
GAGTCTAGCT CTGCTCCCTC CAGTCCTGAT GCTTTGCACA CAGACACAGC TGACCCAGTG 
CTGGGCTGTT CAGAAGAAAG CAAGGTCCGG AGGACGGCCT GCATGTATGG GGCCAACTGC 
TACAGGAGGA ACCCCCTCCA CTTTCAACAC TTCAGCCACC CTGGTGACAG TGACTATGGA 
GAGGTACATG GCACAGATGA AGGTGTGATT GGTGACAGGC CCGAGTGTCC CTATGGAGCG 
TCTTGTTACA GGAAGAACCC CCAGCACAAG ATGGAATATA GGCACAGTGC ACTTCCAGCC 
AGAGTTGCTC TGGATGAAGA TGACGATGAT GTCGGGCAAC CCAGTGACGA TGAGGACGAG 
GAAGACTATG AGCCGACAGA TGAAGATTCT GATTGGCACC CAGGAAAGGA CGATGAGGAG 
CAGGAAGATG TGGATGAGCT TTTGAAAGAA GCAAAAAGGT TTATGAGAAG GAAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001359261.1
CDS	207..1706
Translation	MPSDFFLQPLDGGPRVPVGPGQTVIGRGPLLGITDKRVSRRHAILEVVDSQLRIKPIHRNPCFYQSSEKSQHSPMETQVWSQLHPGDSFSLLLDKYAFRVFSAESEVEMECTLRNSQMLDEDDILSEMQKSPVVNLPDKTTGASQLQGSPEITKTKCPTIDPMSSSGECRAFSEHQPRPTQRKRILPAWMLAESLSDQSLSTPAEGGDKDVIQRSGKAGTCEDRTPGNTSWHGKKRLSPSGNSKSVSAEQDPGKKCRKADQEGPGVSSENVPESSSSNIVKDPDVDIVKTNKQKDGILIEELGEVSKHKAATKPTTNEEGESCARVQSKSPPEKSQGCHPESSSAPSSPDALHTDTADPVLGCSEESKVRRTACMYGANCYRRNPLHFQHFSHPGDSDYGEVHGTDEGVIGDRPECPYGASCYRKNPQHKMEYRHSALPARVALDEDDDDVGQPSDDEDEEDYEPTDEDSDWHPGKDDEEQEDVDELLKEAKRFMRRKK

Target ORF information:

RefSeq Version	NM_001372332.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372332.1

ORF Insert Sequence:

ATGCCTTCGG ACTTCTTTCT GCAGCCTCTG GACGGCGGTC CCCGCGTGCC CGTGGGGCCG 
GGGCAGACTG TGATAGGCCG CGGGCCGCTG CTGGGAATAA CAGACAAAAG AGTATCCAGA 
AGACATGCCA TTCTTGAAGT GGTTGATAGT CAGCTTCGAA TCAAGCCAAT CCATAGGAAT 
CCATGTTTCT ACCAGTCCTC GGAGAAGAGC CAACACTCGC CAATGGAGAC GCAAGTGTGG 
AGCCAGTTGC ATCCCGGGGA TAGCTTTTCC CTGTTACTTG ACAAGTACGC TTTCCGTGTT 
TTCTCTGCAG AATCTGAAGT GGAGATGGAG TGTACTCTGA GAAACAGTCA GATGCTTGAT 
GAAGATGATA TATTGAGTGA GATGCAGAAG TCCCCTGTGG TTAACTTACC TGATAAGACA 
ACTGGTGCCT CACAGCTGCA AGGAAGCCCT GAAATAACCA AGACCAAGTG TCCCACCATA 
GATCCTATGT CCTCTTCAGG TGAATGCAGA GCTTTCAGTG AGCACCAGCC ACGTCCTACC 
CAGAGGAAAA GAATCCTCCC AGCTTGGATG TTGGCGGAAA GTCTAAGTGA CCAGAGCCTC 
TCCACACCAG CTGAGGGGGG AGATAAGGAT GTCATCCAGA GGAGTGGAAA AGCAGGAACC 
TGTGAAGATA GAACCCCAGG AAACACATCA TGGCATGGGA AAAAGCGGCT TAGTCCATCA 
GGAAATTCAA AAAGTGTGTC AGCGGAACAA GACCCTGGGA AGAAGTGCAG AAAGGCTGAC 
CAAGAAGGAC CGGGTGTTTC TTCTGAGAAT GTTCCAGAAT CATCATCTTC AAACATAGTA 
AAGGACCCAG ATGTGGATAT TGTGAAGACA AATAAACAGA AGGACGGGAT TTTAATAGAG 
GAACTTGGTG AGGTCTCTAA ACATAAAGCC GCCACCAAAC CAACCACAAA TGAAGAGGGC 
GAGAGTTGTG CACGTGTCCA AAGCAAGTCA CCTCCTGAGA AATCTCAAGG ATGCCATCCT 
GAGTCTAGCT CTGCTCCCTC CAGTCCTGAT GCTTTGCACA CAGACACAGC TGACCCAGTG 
CTGGGCTGTT CAGAAGAAAG CAAGGTCCGG AGGACGGCCT GCATGTATGG GGCCAACTGC 
TACAGGAGGA ACCCCCTCCA CTTTCAACAC TTCAGCCACC CTGGTGACAG TGACTATGGA 
GAGGTACATG GCACAGATGA AGGTGTGATT GGTGACAGGC CCGAGTGTCC CTATGGAGCG 
TCTTGTTACA GGAAGAACCC CCAGCACAAG ATGGAATATA GGCACAGTGC ACTTCCAGCC 
AGAGTTGCTC TGGATGAAGA TGACGATGAT GTCGGGCAAC CCAGTGACGA TGAGGACGAG 
GAAGACTATG AGCCGACAGA TGAAGATTCT GATTGGCACC CAGGAAAGGA CGATGAGGAG 
CAGGAAGATG TGGATGAGCT TTTGAAAGAA GCAAAAAGGT TTATGAGAAG GAAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aplf ( XM_006506657.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506720.1 Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X3, mRNA.

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CloneID	OMu37609
Clone ID Related Accession (Same CDS sequence)	XM_006506657.3 , XM_006506657.4 , XM_006506656.3 , XM_006506656.4
Accession Version	XM_006506657.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	aprataxin and PNK-like factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506657.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC AAGTGTGGAG CCAGTTGCAT CCCGGGGATA GCTTTTCCCT GTTACTTGAC 
AAGTACGCTT TCCGTGTTTT CTCTGCAGAA TCTGAAGTGG AGATGGAGTG TACTCTGAGA 
AACAGTCAGA TGCTTGATGA AGATGATATA TTGAGTGAGA TGCAGAAGTC CCCTGTGGTT 
AACTTACCTG ATAAGACAAC TGGTGCCTCA CAGCTGCAAG GAAGCCCTGA AATAACCAAG 
ACCAAGTGTC CCACCATAGA TCCTATGTCC TCTTCAGGTG AATGCAGAGC TTTCAGTGAG 
CACCAGCCAC GTCCTACCCA GAGGAAAAGA ATCCTCCCAG CTTGGATGTT GGCGGAAAGT 
CTAAGTGACC AGAGCCTCTC CACACCAGCT GAGGGGGGAG ATAAGGATGT CATCCAGAGG 
AGTGGAAAAG CAGGAACCTG TGAAGATAGA ACCCCAGGAA ACACATCATG GCATGGGAAA 
AAGCGGCTTA GTCCATCAGG AAATTCAAAA AGTGTGTCAG CGGAACAAGA CCCTGGGAAG 
AAGTGCAGAA AGGCTGACCA AGAAGGACCG GGTGTTTCTT CTGAGAATGT TCCAGAATCA 
TCATCTTCAA ACATAGTAAA GGACCCAGAT GTGGATATTG TGAAGACAAA TAAACAGAAG 
GACGGGATTT TAATAGAGGA ACTTGGTGAG GTCTCTAAAC ATAAAGCCGC CACCAAACCA 
ACCACAAATG AAGAGGGCGA GAGTTGTGCA CGTGTCCAAA GCAAGTCACC TCCTGAGAAA 
TCTCAAGGAT GCCATCCTGA GTCTAGCTCT GCTCCCTCCA GTCCTGATGC TTTGCACACA 
GACACAGCTG ACCCAGTGCT GGGCTGTTCA GAAGAAAGCA AGGTCCGGAG GACGGCCTGC 
ATGTATGGGG CCAACTGCTA CAGGAGGAAC CCCCTCCACT TTCAACACTT CAGCCACCCT 
GGTGACAGTG ACTATGGAGA GGTACATGGC ACAGATGAAG GTGTGATTGG TGACAGGCCC 
GAGTGTCCCT ATGGAGCGTC TTGTTACAGG AAGAACCCCC AGCACAAGAT GGAATATAGG 
CACAGTGCAC TTCCAGCCAG AGTTGCTCTG GATGAAGATG ACGATGATGT CGGGCAACCC 
AGTGACGATG AGGACGAGGA AGACTATGAG CCGACAGATG AAGATTCTGA TTGGCACCCA 
GGAAAGGACG ATGAGGAGCA GGAAGATGTG GATGAGCTTT TGAAAGAAGC AAAAAGGTTT 
ATGAGAAGGA AAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506720.1
CDS	802..2079
Translation	METQVWSQLHPGDSFSLLLDKYAFRVFSAESEVEMECTLRNSQMLDEDDILSEMQKSPVVNLPDKTTGASQLQGSPEITKTKCPTIDPMSSSGECRAFSEHQPRPTQRKRILPAWMLAESLSDQSLSTPAEGGDKDVIQRSGKAGTCEDRTPGNTSWHGKKRLSPSGNSKSVSAEQDPGKKCRKADQEGPGVSSENVPESSSSNIVKDPDVDIVKTNKQKDGILIEELGEVSKHKAATKPTTNEEGESCARVQSKSPPEKSQGCHPESSSAPSSPDALHTDTADPVLGCSEESKVRRTACMYGANCYRRNPLHFQHFSHPGDSDYGEVHGTDEGVIGDRPECPYGASCYRKNPQHKMEYRHSALPARVALDEDDDDVGQPSDDEDEEDYEPTDEDSDWHPGKDDEEQEDVDELLKEAKRFMRRKK

Target ORF information:

RefSeq Version	XM_006506657.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506657.4

ORF Insert Sequence:

ATGGAGACGC AAGTGTGGAG CCAGTTGCAT CCCGGGGATA GCTTTTCCCT GTTACTTGAC 
AAGTACGCTT TCCGTGTTTT CTCTGCAGAA TCTGAAGTGG AGATGGAGTG TACTCTGAGA 
AACAGTCAGA TGCTTGATGA AGATGATATA TTGAGTGAGA TGCAGAAGTC CCCTGTGGTT 
AACTTACCTG ATAAGACAAC TGGTGCCTCA CAGCTGCAAG GAAGCCCTGA AATAACCAAG 
ACCAAGTGTC CCACCATAGA TCCTATGTCC TCTTCAGGTG AATGCAGAGC TTTCAGTGAG 
CACCAGCCAC GTCCTACCCA GAGGAAAAGA ATCCTCCCAG CTTGGATGTT GGCGGAAAGT 
CTAAGTGACC AGAGCCTCTC CACACCAGCT GAGGGGGGAG ATAAGGATGT CATCCAGAGG 
AGTGGAAAAG CAGGAACCTG TGAAGATAGA ACCCCAGGAA ACACATCATG GCATGGGAAA 
AAGCGGCTTA GTCCATCAGG AAATTCAAAA AGTGTGTCAG CGGAACAAGA CCCTGGGAAG 
AAGTGCAGAA AGGCTGACCA AGAAGGACCG GGTGTTTCTT CTGAGAATGT TCCAGAATCA 
TCATCTTCAA ACATAGTAAA GGACCCAGAT GTGGATATTG TGAAGACAAA TAAACAGAAG 
GACGGGATTT TAATAGAGGA ACTTGGTGAG GTCTCTAAAC ATAAAGCCGC CACCAAACCA 
ACCACAAATG AAGAGGGCGA GAGTTGTGCA CGTGTCCAAA GCAAGTCACC TCCTGAGAAA 
TCTCAAGGAT GCCATCCTGA GTCTAGCTCT GCTCCCTCCA GTCCTGATGC TTTGCACACA 
GACACAGCTG ACCCAGTGCT GGGCTGTTCA GAAGAAAGCA AGGTCCGGAG GACGGCCTGC 
ATGTATGGGG CCAACTGCTA CAGGAGGAAC CCCCTCCACT TTCAACACTT CAGCCACCCT 
GGTGACAGTG ACTATGGAGA GGTACATGGC ACAGATGAAG GTGTGATTGG TGACAGGCCC 
GAGTGTCCCT ATGGAGCGTC TTGTTACAGG AAGAACCCCC AGCACAAGAT GGAATATAGG 
CACAGTGCAC TTCCAGCCAG AGTTGCTCTG GATGAAGATG ACGATGATGT CGGGCAACCC 
AGTGACGATG AGGACGAGGA AGACTATGAG CCGACAGATG AAGATTCTGA TTGGCACCCA 
GGAAAGGACG ATGAGGAGCA GGAAGATGTG GATGAGCTTT TGAAAGAAGC AAAAAGGTTT 
ATGAGAAGGA AAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aplf ( XM_006506656.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506719.1 Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X2, mRNA.

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CloneID	OMu37609
Clone ID Related Accession (Same CDS sequence)	XM_006506657.3 , XM_006506657.4 , XM_006506656.3 , XM_006506656.4
Accession Version	XM_006506656.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	aprataxin and PNK-like factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506656.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC AAGTGTGGAG CCAGTTGCAT CCCGGGGATA GCTTTTCCCT GTTACTTGAC 
AAGTACGCTT TCCGTGTTTT CTCTGCAGAA TCTGAAGTGG AGATGGAGTG TACTCTGAGA 
AACAGTCAGA TGCTTGATGA AGATGATATA TTGAGTGAGA TGCAGAAGTC CCCTGTGGTT 
AACTTACCTG ATAAGACAAC TGGTGCCTCA CAGCTGCAAG GAAGCCCTGA AATAACCAAG 
ACCAAGTGTC CCACCATAGA TCCTATGTCC TCTTCAGGTG AATGCAGAGC TTTCAGTGAG 
CACCAGCCAC GTCCTACCCA GAGGAAAAGA ATCCTCCCAG CTTGGATGTT GGCGGAAAGT 
CTAAGTGACC AGAGCCTCTC CACACCAGCT GAGGGGGGAG ATAAGGATGT CATCCAGAGG 
AGTGGAAAAG CAGGAACCTG TGAAGATAGA ACCCCAGGAA ACACATCATG GCATGGGAAA 
AAGCGGCTTA GTCCATCAGG AAATTCAAAA AGTGTGTCAG CGGAACAAGA CCCTGGGAAG 
AAGTGCAGAA AGGCTGACCA AGAAGGACCG GGTGTTTCTT CTGAGAATGT TCCAGAATCA 
TCATCTTCAA ACATAGTAAA GGACCCAGAT GTGGATATTG TGAAGACAAA TAAACAGAAG 
GACGGGATTT TAATAGAGGA ACTTGGTGAG GTCTCTAAAC ATAAAGCCGC CACCAAACCA 
ACCACAAATG AAGAGGGCGA GAGTTGTGCA CGTGTCCAAA GCAAGTCACC TCCTGAGAAA 
TCTCAAGGAT GCCATCCTGA GTCTAGCTCT GCTCCCTCCA GTCCTGATGC TTTGCACACA 
GACACAGCTG ACCCAGTGCT GGGCTGTTCA GAAGAAAGCA AGGTCCGGAG GACGGCCTGC 
ATGTATGGGG CCAACTGCTA CAGGAGGAAC CCCCTCCACT TTCAACACTT CAGCCACCCT 
GGTGACAGTG ACTATGGAGA GGTACATGGC ACAGATGAAG GTGTGATTGG TGACAGGCCC 
GAGTGTCCCT ATGGAGCGTC TTGTTACAGG AAGAACCCCC AGCACAAGAT GGAATATAGG 
CACAGTGCAC TTCCAGCCAG AGTTGCTCTG GATGAAGATG ACGATGATGT CGGGCAACCC 
AGTGACGATG AGGACGAGGA AGACTATGAG CCGACAGATG AAGATTCTGA TTGGCACCCA 
GGAAAGGACG ATGAGGAGCA GGAAGATGTG GATGAGCTTT TGAAAGAAGC AAAAAGGTTT 
ATGAGAAGGA AAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506719.1
CDS	238..1515
Translation	METQVWSQLHPGDSFSLLLDKYAFRVFSAESEVEMECTLRNSQMLDEDDILSEMQKSPVVNLPDKTTGASQLQGSPEITKTKCPTIDPMSSSGECRAFSEHQPRPTQRKRILPAWMLAESLSDQSLSTPAEGGDKDVIQRSGKAGTCEDRTPGNTSWHGKKRLSPSGNSKSVSAEQDPGKKCRKADQEGPGVSSENVPESSSSNIVKDPDVDIVKTNKQKDGILIEELGEVSKHKAATKPTTNEEGESCARVQSKSPPEKSQGCHPESSSAPSSPDALHTDTADPVLGCSEESKVRRTACMYGANCYRRNPLHFQHFSHPGDSDYGEVHGTDEGVIGDRPECPYGASCYRKNPQHKMEYRHSALPARVALDEDDDDVGQPSDDEDEEDYEPTDEDSDWHPGKDDEEQEDVDELLKEAKRFMRRKK

Target ORF information:

RefSeq Version	XM_006506656.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506656.4

ORF Insert Sequence:

ATGGAGACGC AAGTGTGGAG CCAGTTGCAT CCCGGGGATA GCTTTTCCCT GTTACTTGAC 
AAGTACGCTT TCCGTGTTTT CTCTGCAGAA TCTGAAGTGG AGATGGAGTG TACTCTGAGA 
AACAGTCAGA TGCTTGATGA AGATGATATA TTGAGTGAGA TGCAGAAGTC CCCTGTGGTT 
AACTTACCTG ATAAGACAAC TGGTGCCTCA CAGCTGCAAG GAAGCCCTGA AATAACCAAG 
ACCAAGTGTC CCACCATAGA TCCTATGTCC TCTTCAGGTG AATGCAGAGC TTTCAGTGAG 
CACCAGCCAC GTCCTACCCA GAGGAAAAGA ATCCTCCCAG CTTGGATGTT GGCGGAAAGT 
CTAAGTGACC AGAGCCTCTC CACACCAGCT GAGGGGGGAG ATAAGGATGT CATCCAGAGG 
AGTGGAAAAG CAGGAACCTG TGAAGATAGA ACCCCAGGAA ACACATCATG GCATGGGAAA 
AAGCGGCTTA GTCCATCAGG AAATTCAAAA AGTGTGTCAG CGGAACAAGA CCCTGGGAAG 
AAGTGCAGAA AGGCTGACCA AGAAGGACCG GGTGTTTCTT CTGAGAATGT TCCAGAATCA 
TCATCTTCAA ACATAGTAAA GGACCCAGAT GTGGATATTG TGAAGACAAA TAAACAGAAG 
GACGGGATTT TAATAGAGGA ACTTGGTGAG GTCTCTAAAC ATAAAGCCGC CACCAAACCA 
ACCACAAATG AAGAGGGCGA GAGTTGTGCA CGTGTCCAAA GCAAGTCACC TCCTGAGAAA 
TCTCAAGGAT GCCATCCTGA GTCTAGCTCT GCTCCCTCCA GTCCTGATGC TTTGCACACA 
GACACAGCTG ACCCAGTGCT GGGCTGTTCA GAAGAAAGCA AGGTCCGGAG GACGGCCTGC 
ATGTATGGGG CCAACTGCTA CAGGAGGAAC CCCCTCCACT TTCAACACTT CAGCCACCCT 
GGTGACAGTG ACTATGGAGA GGTACATGGC ACAGATGAAG GTGTGATTGG TGACAGGCCC 
GAGTGTCCCT ATGGAGCGTC TTGTTACAGG AAGAACCCCC AGCACAAGAT GGAATATAGG 
CACAGTGCAC TTCCAGCCAG AGTTGCTCTG GATGAAGATG ACGATGATGT CGGGCAACCC 
AGTGACGATG AGGACGAGGA AGACTATGAG CCGACAGATG AAGATTCTGA TTGGCACCCA 
GGAAAGGACG ATGAGGAGCA GGAAGATGTG GATGAGCTTT TGAAAGAAGC AAAAAGGTTT 
ATGAGAAGGA AAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aplf ( XM_017321755.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177244.1 Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X1, mRNA.

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CloneID	OMu81803
Clone ID Related Accession (Same CDS sequence)	XM_017321755.1 , NM_001372332.1
Accession Version	XM_017321755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	aprataxin and PNK-like factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCGG ACTTCTTTCT GCAGCCTCTG GACGGCGGTC CCCGCGTGCC CGTGGGGCCG 
GGGCAGACTG TGATAGGCCG CGGGCCGCTG CTGGGAATAA CAGACAAAAG AGTATCCAGA 
AGACATGCCA TTCTTGAAGT GGTTGATAGT CAGCTTCGAA TCAAGCCAAT CCATAGGAAT 
CCATGTTTCT ACCAGTCCTC GGAGAAGAGC CAACACTCGC CAATGGAGAC GCAAGTGTGG 
AGCCAGTTGC ATCCCGGGGA TAGCTTTTCC CTGTTACTTG ACAAGTACGC TTTCCGTGTT 
TTCTCTGCAG AATCTGAAGT GGAGATGGAG TGTACTCTGA GAAACAGTCA GATGCTTGAT 
GAAGATGATA TATTGAGTGA GATGCAGAAG TCCCCTGTGG TTAACTTACC TGATAAGACA 
ACTGGTGCCT CACAGCTGCA AGGAAGCCCT GAAATAACCA AGACCAAGTG TCCCACCATA 
GATCCTATGT CCTCTTCAGG TGAATGCAGA GCTTTCAGTG AGCACCAGCC ACGTCCTACC 
CAGAGGAAAA GAATCCTCCC AGCTTGGATG TTGGCGGAAA GTCTAAGTGA CCAGAGCCTC 
TCCACACCAG CTGAGGGGGG AGATAAGGAT GTCATCCAGA GGAGTGGAAA AGCAGGAACC 
TGTGAAGATA GAACCCCAGG AAACACATCA TGGCATGGGA AAAAGCGGCT TAGTCCATCA 
GGAAATTCAA AAAGTGTGTC AGCGGAACAA GACCCTGGGA AGAAGTGCAG AAAGGCTGAC 
CAAGAAGGAC CGGGTGTTTC TTCTGAGAAT GTTCCAGAAT CATCATCTTC AAACATAGTA 
AAGGACCCAG ATGTGGATAT TGTGAAGACA AATAAACAGA AGGACGGGAT TTTAATAGAG 
GAACTTGGTG AGGTCTCTAA ACATAAAGCC GCCACCAAAC CAACCACAAA TGAAGAGGGC 
GAGAGTTGTG CACGTGTCCA AAGCAAGTCA CCTCCTGAGA AATCTCAAGG ATGCCATCCT 
GAGTCTAGCT CTGCTCCCTC CAGTCCTGAT GCTTTGCACA CAGACACAGC TGACCCAGTG 
CTGGGCTGTT CAGAAGAAAG CAAGGTCCGG AGGACGGCCT GCATGTATGG GGCCAACTGC 
TACAGGAGGA ACCCCCTCCA CTTTCAACAC TTCAGCCACC CTGGTGACAG TGACTATGGA 
GAGGTACATG GCACAGATGA AGGTGTGATT GGTGACAGGC CCGAGTGTCC CTATGGAGCG 
TCTTGTTACA GGAAGAACCC CCAGCACAAG ATGGAATATA GGCACAGTGC ACTTCCAGCC 
AGAGTTGCTC TGGATGAAGA TGACGATGAT GTCGGGCAAC CCAGTGACGA TGAGGACGAG 
GAAGACTATG AGCCGACAGA TGAAGATTCT GATTGGCACC CAGGAAAGGA CGATGAGGAG 
CAGGAAGATG TGGATGAGCT TTTGAAAGAA GCAAAAAGGT TTATGAGAAG GAAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177244.1
CDS	249..1748
Translation	MPSDFFLQPLDGGPRVPVGPGQTVIGRGPLLGITDKRVSRRHAILEVVDSQLRIKPIHRNPCFYQSSEKSQHSPMETQVWSQLHPGDSFSLLLDKYAFRVFSAESEVEMECTLRNSQMLDEDDILSEMQKSPVVNLPDKTTGASQLQGSPEITKTKCPTIDPMSSSGECRAFSEHQPRPTQRKRILPAWMLAESLSDQSLSTPAEGGDKDVIQRSGKAGTCEDRTPGNTSWHGKKRLSPSGNSKSVSAEQDPGKKCRKADQEGPGVSSENVPESSSSNIVKDPDVDIVKTNKQKDGILIEELGEVSKHKAATKPTTNEEGESCARVQSKSPPEKSQGCHPESSSAPSSPDALHTDTADPVLGCSEESKVRRTACMYGANCYRRNPLHFQHFSHPGDSDYGEVHGTDEGVIGDRPECPYGASCYRKNPQHKMEYRHSALPARVALDEDDDDVGQPSDDEDEEDYEPTDEDSDWHPGKDDEEQEDVDELLKEAKRFMRRKK

Target ORF information:

RefSeq Version	XM_017321755.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus aprataxin and PNKP like factor (Aplf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321755.1

ORF Insert Sequence:

ATGCCTTCGG ACTTCTTTCT GCAGCCTCTG GACGGCGGTC CCCGCGTGCC CGTGGGGCCG 
GGGCAGACTG TGATAGGCCG CGGGCCGCTG CTGGGAATAA CAGACAAAAG AGTATCCAGA 
AGACATGCCA TTCTTGAAGT GGTTGATAGT CAGCTTCGAA TCAAGCCAAT CCATAGGAAT 
CCATGTTTCT ACCAGTCCTC GGAGAAGAGC CAACACTCGC CAATGGAGAC GCAAGTGTGG 
AGCCAGTTGC ATCCCGGGGA TAGCTTTTCC CTGTTACTTG ACAAGTACGC TTTCCGTGTT 
TTCTCTGCAG AATCTGAAGT GGAGATGGAG TGTACTCTGA GAAACAGTCA GATGCTTGAT 
GAAGATGATA TATTGAGTGA GATGCAGAAG TCCCCTGTGG TTAACTTACC TGATAAGACA 
ACTGGTGCCT CACAGCTGCA AGGAAGCCCT GAAATAACCA AGACCAAGTG TCCCACCATA 
GATCCTATGT CCTCTTCAGG TGAATGCAGA GCTTTCAGTG AGCACCAGCC ACGTCCTACC 
CAGAGGAAAA GAATCCTCCC AGCTTGGATG TTGGCGGAAA GTCTAAGTGA CCAGAGCCTC 
TCCACACCAG CTGAGGGGGG AGATAAGGAT GTCATCCAGA GGAGTGGAAA AGCAGGAACC 
TGTGAAGATA GAACCCCAGG AAACACATCA TGGCATGGGA AAAAGCGGCT TAGTCCATCA 
GGAAATTCAA AAAGTGTGTC AGCGGAACAA GACCCTGGGA AGAAGTGCAG AAAGGCTGAC 
CAAGAAGGAC CGGGTGTTTC TTCTGAGAAT GTTCCAGAAT CATCATCTTC AAACATAGTA 
AAGGACCCAG ATGTGGATAT TGTGAAGACA AATAAACAGA AGGACGGGAT TTTAATAGAG 
GAACTTGGTG AGGTCTCTAA ACATAAAGCC GCCACCAAAC CAACCACAAA TGAAGAGGGC 
GAGAGTTGTG CACGTGTCCA AAGCAAGTCA CCTCCTGAGA AATCTCAAGG ATGCCATCCT 
GAGTCTAGCT CTGCTCCCTC CAGTCCTGAT GCTTTGCACA CAGACACAGC TGACCCAGTG 
CTGGGCTGTT CAGAAGAAAG CAAGGTCCGG AGGACGGCCT GCATGTATGG GGCCAACTGC 
TACAGGAGGA ACCCCCTCCA CTTTCAACAC TTCAGCCACC CTGGTGACAG TGACTATGGA 
GAGGTACATG GCACAGATGA AGGTGTGATT GGTGACAGGC CCGAGTGTCC CTATGGAGCG 
TCTTGTTACA GGAAGAACCC CCAGCACAAG ATGGAATATA GGCACAGTGC ACTTCCAGCC 
AGAGTTGCTC TGGATGAAGA TGACGATGAT GTCGGGCAAC CCAGTGACGA TGAGGACGAG 
GAAGACTATG AGCCGACAGA TGAAGATTCT GATTGGCACC CAGGAAAGGA CGATGAGGAG 
CAGGAAGATG TGGATGAGCT TTTGAAAGAA GCAAAAAGGT TTATGAGAAG GAAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone chaperone APLF regulates induction of pluripotency in murine fibroblasts.
Journal of cell science129(24)4576-4591(2016 12)
Syed KM,Joseph S,Mukherjee A,Majumder A,Teixeira JM,Dutta D,Pillai MR

PARP-3 and APLF function together to accelerate nonhomologous end-joining.
Molecular cell41(1)33-45(2011 Jan)
Rulten SL,Fisher AE,Robert I,Zuma MC,Rouleau M,Ju L,Poirier G,Reina-San-Martin B,Caldecott KW

Quantitative genetics of age-related retinal degeneration: a second F1 intercross between the A/J and C57BL/6 strains.
Molecular vision1379-85(2007 Jan)
Danciger M,Yang H,Ralston R,Liu Y,Matthes MT,Peirce J,Lavail MM

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Aplf cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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