Ankle2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ankle2
Entrez Gene ID	71782
Full Name	ankyrin repeat and LEM domain containing 2
Synonyms	1110001J12Rik,AI661024,D5Ertd585e
General protein information	Preferred Names ankyrin repeat and LEM domain containing 2 Names ankyrin repeat and LEM domain-containing protein 2 LEM domain-containing protein 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 F\|5 53.4 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001253814.1	NP_001240743.1	ankyrin repeat and LEM domain-containing protein 2 isoform 1
NM_027922.2	NP_082198.1	ankyrin repeat and LEM domain-containing protein 2 isoform 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ankle2	NP_001041366.1
Homo sapiens (human)	ANKLE2	NP_055929.1
Bos taurus (cattle)	ANKLE2	XP_002694494.2
	ANKLE2	XP_001087028.1
Danio rerio (zebrafish)	ankle2	NP_001119867.1
Xenopus tropicalis (tropical clawed frog)	ankle2	XP_004910629.1
Canis lupus familiaris (dog)	ANKLE2	XP_852228.2
Pan troglodytes (chimpanzee)	ANKLE2	XP_001152324.2
Mus musculus (house mouse)	Ankle2	NP_001240743.1
Gallus gallus (chicken)	ANKLE2	XP_415084.4

Gene Ontology
Bibliography

Related articles in PubMed

Zinc finger protein Zfp335 is required for the formation of the na?ve T cell compartment.
Han BY, Wu S, Foo CS, Horton RM, Jenne CN, Watson SR, Whittle B, Goodnow CC, Cyster JG
eLife3(2014 Oct)

Tagging genes with cassette-exchange sites.
Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M, Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A, Cortese R
Nucleic acids research33(4)e44(2005 Mar)

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Restoring the expression of one Zfp335 target, Ankle2, partially rescues T cell maturation.
Title: Zinc finger protein Zfp335 is required for the formation of the na?ve T cell compartment.

The following Ankle2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ankle2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05641	NM_001253814.1 Latest version!	Mus musculus ankyrin repeat and LEM domain containing 2 (Ankle2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu04795	NM_027922.2 Latest version!	Mus musculus ankyrin repeat and LEM domain containing 2 (Ankle2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ankle2 ( NM_001253814.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001240743.1 Mus musculus ankyrin repeat and LEM domain containing 2 (Ankle2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05641
Clone ID Related Accession (Same CDS sequence)	NM_001253814.1
Accession Version	NM_001253814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132187.1, AC118260.7, BC065071.1 and CD538245.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK132187.1, SRR7652917.993114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTGTGGC AGCGGCTGGC TGTGGTGGAG TGGGCGGCCC TGGCCTGGGA GCTCCTGGGC 
GCCTCGGTGC TGTTCATCGC CGTGCGCTGG CTGGTCCGCC GGCTAGAGAA GCGGCCGCGG 
GACCTGAACC GTTGCGGGAC GCTCTCCTCT CCGCCCAGCG CGTCTGAAGC GGTGGCCGCA 
CAGCCAGGTG AAGTGACAAT GGATGCCATG ATGGCTCGAC TGAAACTCTT GAATCCAGAT 
GACCTTAGAA AAGAAGTTAT GAAAGCTGGA CTTAAATGTG GACCCATTAC ATCAACAACA 
AGATTTATTT TTGAGAAGAA ATTAGCTCAG GCCTTACTGG AACAAGGTGG ATTGCTGACC 
TCTTCCCTTC CCAAGCCCAG CGCGGTTACT GCCATGGCAT TTATCCAGGG CACCTCAAGG 
ACTCCTCCAT CTGTGGACGG GAAACAAACT CAGCAGGCCT GTTTTTCTGA AGACAGAGAT 
TTTGGTTACA GTGTGGGCTT GAACCCTCCA GAAGAGGAAG CGGTAGCATC CAGTGTCCAC 
CCAGTACCCT TCAGTGCCTC TACCAGGAAT GACAACCACA AGGCTGGAGT GACAGCTCCT 
AAGGAGCCAC TTGTGTACTA TGGAGTGTGT CCAGTATATG AGGATGGCCC TGTGAGACAT 
GAGAGGATCC ATGTTTACGA AGATAAAAAG GAAGCTTTAC AAGCTGCCAA GTTGATCAAA 
GGGTCCCGGT TTAAAGCTTT TCGAACAAGA GAAGATGCTG AGAAATTTGC TAGAGGGATA 
TGTGATTATT TACCTTCTCC CAACAAAACT ACGCCCTTAC TGTCTCCTGT GAAGGCAGTG 
CCCCTCGGTG GCAGTGATGG GCTGAAAGCA GATGGCTTGT GCCTGGCTGA ATCAGAAACA 
GTAAACAAAG AACGAGCAAA CAGTTACAAA AACCCCCGTA CCCAGGACCT CACGGCCAAG 
CTGCGGAAAG CTGTCGAAAA CGGGGAGGAA CACACCTTTT CTGATCTTAT CTGGAGTAAT 
CCTCGATATC TAATTGGTTC TGGGGACAAC CCAACCATTG TACAAGAAGG ATGTAGGTAC 
AATGTCATGC ATGTTGCTGC CAAAGAGAAC CAGGCTTCCA TGTGCCAGCT GACATTAGAG 
ACTCTGGAGA ACCCTGAGTT TATGCGTCTG ATGTACCCAG ATGACAACAT GGACATGCTA 
CAGAAGCGCA TCCTCTACGT TGTTGACCTG TATCTTAATA CTCCTGACAA AGTGGGCTTT 
GATACACCAC TGCATTTTGC TTGTAAATTT GGAAATGTGG ATGTAGTCAA TGTGCTTTCT 
TCACACCCTT TGATTGTGAA AAACCGAAAG AATAAATATG GTAAAACACC TGAAGAAGTA 
ATTTGTGAAA GAAGCCAAAA CAAATCTCCA GCACTGAAAG AAAGGATTAG AGAATACTTA 
ATGGGTCACT ACTATGTGCC ACTCCTGAGA GCAGAAGACA CTTCTCCTGT CATTGGGGAG 
CTGTGGTCCT CAGACCAGAA AGCTGAAGCC TCAAATACTG CCCACTGTAG AAGCAGCCCC 
AGAGATCCTG TGATGACACT GAGAGCTTTC GTGGGACCTC TGAGTCCATC CAAAGCAGAA 
GATTTTCGAA AGCTCTGGAA AACTCCACCT CGAAAGAAAG CAGGCTTCTT CCACAGCATC 
AGAAAGTCTG ACCCAGAACG AGGCATTGAG AGAGTTGGAA GGGAGCTAGC TCATGAGCTG 
GGGTATCCCT GGGTTGAATA CTGGGAATTT CTGGGATGTT TTGTTGATCT GTCTTCCCAG 
GAAGGCTTGC AAAGACTAGA AGAATACCTT ATCCAGAAGG AGTTAAGCAA AAAGGCTCAA 
CAAGAAATAC GAGAAAATGA AGGCTGCCTT CAGGACAGAA CCTCAGATTT TGGCAGTGGA 
AAGAAGTACA GCAACTCCAT CTCCGTGGGA GCATTTCTAG ATGGGGATGA TGACAGTAGT 
TTGGAAGAAA TTAAAAACCA GCAAAATACA GTTCCAAGTC AGAGTCAGCC CACAACTGAT 
AAGTTCCAAA CCTCTAAAAG TGGTTCCCTT CCCTTGGGAC AGAAGGTCGA CCCTGGAGAA 
ACCTCAGTTG GAACTTACCC AGATAAAGGC AGAAATGGGT TTTGTCACCC TCTAAACCAC 
AGGACTGCAG ATGGAAGGGG ACTAGAGGCC ACCAATGGGG AGGAGGCCCT TCCACCACCT 
GTTTCTGTTT TGACTCAAGA GTTGAATAAA CTGAATTTGC AAAGCCTGGG AGACAGCCTT 
CATGAGACAC CAGATAAAAA CGGGAAACTA GAGGATGAGG TGCTTCCTTC AAGAAAGGGT 
GCAGCAGACA GTGACTTGCT GGCATCTCCT CCTGCCATAG CAAGCCTTGG AAAGAAGCAA 
GTCAGGACCA ACACTGAGGT GTCAGAGGCA ATGGCTGAAA TGTCTTTGGG CCCTAAGAGC 
CCCCAACTTG GAGTTCAGGC TGGTCTGGAA CCTATACTCT CATCTGCCAC GGTAGATTCC 
ACCAAGAGGC TCTTCCTGTC TGGAGAGGAA CCATCAAAAC TAGATCGAGA TGTTTTAGCA 
GCTCTTGAGT GTGCGAATAT TGACCCTGGT CTGTACCCAG CAATCCACAG ATGGAAAAGC 
ACTGTCATGT GCTACTCGCC CTCAGACAGA CAGAGTTGGC CAAGTCCTGC ACTCAAAGGG 
AAGTTCACAA CTGAGTTGGT GGATCTCGAT TGCTCTCATA GCTGCTCAGG ACGATGCAGT 
CCAGCTGGTA GCAGCCCCAG TAAGCCTGGC CACACCTCCT CCTCTTCTGG CCTGCACAGC 
CCTGGACGCT ACAGTCCAGC ACATGGGAGA CACTTCCAAA GAGTGGCACA CGTGGCCCGA 
CTTGCAGCTC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240743.1
CDS	234..3128
Translation	MLWQRLAVVEWAALAWELLGASVLFIAVRWLVRRLEKRPRDLNRCGTLSSPPSASEAVAAQPGEVTMDAMMARLKLLNPDDLRKEVMKAGLKCGPITSTTRFIFEKKLAQALLEQGGLLTSSLPKPSAVTAMAFIQGTSRTPPSVDGKQTQQACFSEDRDFGYSVGLNPPEEEAVASSVHPVPFSASTRNDNHKAGVTAPKEPLVYYGVCPVYEDGPVRHERIHVYEDKKEALQAAKLIKGSRFKAFRTREDAEKFARGICDYLPSPNKTTPLLSPVKAVPLGGSDGLKADGLCLAESETVNKERANSYKNPRTQDLTAKLRKAVENGEEHTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQASMCQLTLETLENPEFMRLMYPDDNMDMLQKRILYVVDLYLNTPDKVGFDTPLHFACKFGNVDVVNVLSSHPLIVKNRKNKYGKTPEEVICERSQNKSPALKERIREYLMGHYYVPLLRAEDTSPVIGELWSSDQKAEASNTAHCRSSPRDPVMTLRAFVGPLSPSKAEDFRKLWKTPPRKKAGFFHSIRKSDPERGIERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLIQKELSKKAQQEIRENEGCLQDRTSDFGSGKKYSNSISVGAFLDGDDDSSLEEIKNQQNTVPSQSQPTTDKFQTSKSGSLPLGQKVDPGETSVGTYPDKGRNGFCHPLNHRTADGRGLEATNGEEALPPPVSVLTQELNKLNLQSLGDSLHETPDKNGKLEDEVLPSRKGAADSDLLASPPAIASLGKKQVRTNTEVSEAMAEMSLGPKSPQLGVQAGLEPILSSATVDSTKRLFLSGEEPSKLDRDVLAALECANIDPGLYPAIHRWKSTVMCYSPSDRQSWPSPALKGKFTTELVDLDCSHSCSGRCSPAGSSPSKPGHTSSSSGLHSPGRYSPAHGRHFQRVAHVARLAAL

Target ORF information:

RefSeq Version	NM_001253814.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat and LEM domain containing 2 (Ankle2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253814.1

ORF Insert Sequence:

ATGCTGTGGC AGCGGCTGGC TGTGGTGGAG TGGGCGGCCC TGGCCTGGGA GCTCCTGGGC 
GCCTCGGTGC TGTTCATCGC CGTGCGCTGG CTGGTCCGCC GGCTAGAGAA GCGGCCGCGG 
GACCTGAACC GTTGCGGGAC GCTCTCCTCT CCGCCCAGCG CGTCTGAAGC GGTGGCCGCA 
CAGCCAGGTG AAGTGACAAT GGATGCCATG ATGGCTCGAC TGAAACTCTT GAATCCAGAT 
GACCTTAGAA AAGAAGTTAT GAAAGCTGGA CTTAAATGTG GACCCATTAC ATCAACAACA 
AGATTTATTT TTGAGAAGAA ATTAGCTCAG GCCTTACTGG AACAAGGTGG ATTGCTGACC 
TCTTCCCTTC CCAAGCCCAG CGCGGTTACT GCCATGGCAT TTATCCAGGG CACCTCAAGG 
ACTCCTCCAT CTGTGGACGG GAAACAAACT CAGCAGGCCT GTTTTTCTGA AGACAGAGAT 
TTTGGTTACA GTGTGGGCTT GAACCCTCCA GAAGAGGAAG CGGTAGCATC CAGTGTCCAC 
CCAGTACCCT TCAGTGCCTC TACCAGGAAT GACAACCACA AGGCTGGAGT GACAGCTCCT 
AAGGAGCCAC TTGTGTACTA TGGAGTGTGT CCAGTATATG AGGATGGCCC TGTGAGACAT 
GAGAGGATCC ATGTTTACGA AGATAAAAAG GAAGCTTTAC AAGCTGCCAA GTTGATCAAA 
GGGTCCCGGT TTAAAGCTTT TCGAACAAGA GAAGATGCTG AGAAATTTGC TAGAGGGATA 
TGTGATTATT TACCTTCTCC CAACAAAACT ACGCCCTTAC TGTCTCCTGT GAAGGCAGTG 
CCCCTCGGTG GCAGTGATGG GCTGAAAGCA GATGGCTTGT GCCTGGCTGA ATCAGAAACA 
GTAAACAAAG AACGAGCAAA CAGTTACAAA AACCCCCGTA CCCAGGACCT CACGGCCAAG 
CTGCGGAAAG CTGTCGAAAA CGGGGAGGAA CACACCTTTT CTGATCTTAT CTGGAGTAAT 
CCTCGATATC TAATTGGTTC TGGGGACAAC CCAACCATTG TACAAGAAGG ATGTAGGTAC 
AATGTCATGC ATGTTGCTGC CAAAGAGAAC CAGGCTTCCA TGTGCCAGCT GACATTAGAG 
ACTCTGGAGA ACCCTGAGTT TATGCGTCTG ATGTACCCAG ATGACAACAT GGACATGCTA 
CAGAAGCGCA TCCTCTACGT TGTTGACCTG TATCTTAATA CTCCTGACAA AGTGGGCTTT 
GATACACCAC TGCATTTTGC TTGTAAATTT GGAAATGTGG ATGTAGTCAA TGTGCTTTCT 
TCACACCCTT TGATTGTGAA AAACCGAAAG AATAAATATG GTAAAACACC TGAAGAAGTA 
ATTTGTGAAA GAAGCCAAAA CAAATCTCCA GCACTGAAAG AAAGGATTAG AGAATACTTA 
ATGGGTCACT ACTATGTGCC ACTCCTGAGA GCAGAAGACA CTTCTCCTGT CATTGGGGAG 
CTGTGGTCCT CAGACCAGAA AGCTGAAGCC TCAAATACTG CCCACTGTAG AAGCAGCCCC 
AGAGATCCTG TGATGACACT GAGAGCTTTC GTGGGACCTC TGAGTCCATC CAAAGCAGAA 
GATTTTCGAA AGCTCTGGAA AACTCCACCT CGAAAGAAAG CAGGCTTCTT CCACAGCATC 
AGAAAGTCTG ACCCAGAACG AGGCATTGAG AGAGTTGGAA GGGAGCTAGC TCATGAGCTG 
GGGTATCCCT GGGTTGAATA CTGGGAATTT CTGGGATGTT TTGTTGATCT GTCTTCCCAG 
GAAGGCTTGC AAAGACTAGA AGAATACCTT ATCCAGAAGG AGTTAAGCAA AAAGGCTCAA 
CAAGAAATAC GAGAAAATGA AGGCTGCCTT CAGGACAGAA CCTCAGATTT TGGCAGTGGA 
AAGAAGTACA GCAACTCCAT CTCCGTGGGA GCATTTCTAG ATGGGGATGA TGACAGTAGT 
TTGGAAGAAA TTAAAAACCA GCAAAATACA GTTCCAAGTC AGAGTCAGCC CACAACTGAT 
AAGTTCCAAA CCTCTAAAAG TGGTTCCCTT CCCTTGGGAC AGAAGGTCGA CCCTGGAGAA 
ACCTCAGTTG GAACTTACCC AGATAAAGGC AGAAATGGGT TTTGTCACCC TCTAAACCAC 
AGGACTGCAG ATGGAAGGGG ACTAGAGGCC ACCAATGGGG AGGAGGCCCT TCCACCACCT 
GTTTCTGTTT TGACTCAAGA GTTGAATAAA CTGAATTTGC AAAGCCTGGG AGACAGCCTT 
CATGAGACAC CAGATAAAAA CGGGAAACTA GAGGATGAGG TGCTTCCTTC AAGAAAGGGT 
GCAGCAGACA GTGACTTGCT GGCATCTCCT CCTGCCATAG CAAGCCTTGG AAAGAAGCAA 
GTCAGGACCA ACACTGAGGT GTCAGAGGCA ATGGCTGAAA TGTCTTTGGG CCCTAAGAGC 
CCCCAACTTG GAGTTCAGGC TGGTCTGGAA CCTATACTCT CATCTGCCAC GGTAGATTCC 
ACCAAGAGGC TCTTCCTGTC TGGAGAGGAA CCATCAAAAC TAGATCGAGA TGTTTTAGCA 
GCTCTTGAGT GTGCGAATAT TGACCCTGGT CTGTACCCAG CAATCCACAG ATGGAAAAGC 
ACTGTCATGT GCTACTCGCC CTCAGACAGA CAGAGTTGGC CAAGTCCTGC ACTCAAAGGG 
AAGTTCACAA CTGAGTTGGT GGATCTCGAT TGCTCTCATA GCTGCTCAGG ACGATGCAGT 
CCAGCTGGTA GCAGCCCCAG TAAGCCTGGC CACACCTCCT CCTCTTCTGG CCTGCACAGC 
CCTGGACGCT ACAGTCCAGC ACATGGGAGA CACTTCCAAA GAGTGGCACA CGTGGCCCGA 
CTTGCAGCTC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankle2 ( NM_027922.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082198.1 Mus musculus ankyrin repeat and LEM domain containing 2 (Ankle2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu04795
Clone ID Related Accession (Same CDS sequence)	NM_027922.2
Accession Version	NM_027922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132187.1, BC065071.1 and CD538245.1. On Dec 7, 2011 this sequence version replaced NM_027922.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC065071.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGCTGTGGC AGCGGCTGGC TGTGGTGGAG TGGGCGGCCC TGGCCTGGGA GCTCCTGGGC 
GCCTCGGTGC TGTTCATCGC CGTGCGCTGG CTGGTCCGCC GGCTAGAGAA GCGGCCGCGG 
GACCTGAACC GTTGCGGGAC GCTCTCCTCT CCGCCCAGCG CGTCTGAAGC GGTGGCCGCA 
CAGCCAGGTG AAGTGACAAT GGATGCCATG ATGGCTCGAC TGAAACTCTT GAATCCAGAT 
GACCTTAGAA AAGAAGTTAT GAAAGCTGGA CTTAAATGTG GACCCATTAC ATCAACAACA 
AGATTTATTT TTGAGAAGAA ATTAGCTCAG GCCTTACTGG AACAAGGTGG ATTGCTGACC 
TCTTCCCTTC CCAAGCCCAG CGCGGTTACT GCCATGGCAT TTATCCAGGG CACCTCAAGG 
ACTCCTCCAT CTGTGGACGG GAAACAAACT CAGCAGGCCT GTTTTTCTGA AGACAGAGAT 
TTTGGTTACA GTGTGGGCTT GAACCCTCCA GAAGAGGAAG CGGTAGCATC CAGTGTCCAC 
CCAGTACCCT TCAGTGCCTC TACCAGGAAT GACAACCACA AGGCTGGAGT GACAGCTCCT 
AAGGAGCCAC TTGTGTACTA TGGAGTGTGT CCAGTATATG AGGATGGCCC TGTGAGACAT 
GAGAGGATCC ATGTTTACGA AGATAAAAAG GAAGCTTTAC AAGCTGCCAA GTTGATCAAA 
GGGTCCCGGT TTAAAGCTTT TCGAACAAGA GAAGATGCTG AGAAATTTGC TAGAGGGATA 
TGTGATTATT TACCTTCTCC CAACAAAACT ACGCCCTTAC TGTCTCCTGT GAAGGCAGTG 
CCCCTCGGTG GCAGTGATGG GCTGAAAGAT GGCTTGTGCC TGGCTGAATC AGAAACAGTA 
AACAAAGAAC GAGCAAACAG TTACAAAAAC CCCCGTACCC AGGACCTCAC GGCCAAGCTG 
CGGAAAGCTG TCGAAAACGG GGAGGAACAC ACCTTTTCTG ATCTTATCTG GAGTAATCCT 
CGATATCTAA TTGGTTCTGG GGACAACCCA ACCATTGTAC AAGAAGGATG TAGGTACAAT 
GTCATGCATG TTGCTGCCAA AGAGAACCAG GCTTCCATGT GCCAGCTGAC ATTAGAGACT 
CTGGAGAACC CTGAGTTTAT GCGTCTGATG TACCCAGATG ACAACATGGA CATGCTACAG 
AAGCGCATCC TCTACGTTGT TGACCTGTAT CTTAATACTC CTGACAAAGT GGGCTTTGAT 
ACACCACTGC ATTTTGCTTG TAAATTTGGA AATGTGGATG TAGTCAATGT GCTTTCTTCA 
CACCCTTTGA TTGTGAAAAA CCGAAAGAAT AAATATGGTA AAACACCTGA AGAAGTAATT 
TGTGAAAGAA GCCAAAACAA ATCTCCAGCA CTGAAAGAAA GGATTAGAGA ATACTTAATG 
GGTCACTACT ATGTGCCACT CCTGAGAGCA GAAGACACTT CTCCTGTCAT TGGGGAGCTG 
TGGTCCTCAG ACCAGAAAGC TGAAGCCTCA AATACTGCCC ACTGTAGAAG CAGCCCCAGA 
GATCCTGTGA TGACACTGAG AGCTTTCGTG GGACCTCTGA GTCCATCCAA AGCAGAAGAT 
TTTCGAAAGC TCTGGAAAAC TCCACCTCGA AAGAAAGCAG GCTTCTTCCA CAGCATCAGA 
AAGTCTGACC CAGAACGAGG CATTGAGAGA GTTGGAAGGG AGCTAGCTCA TGAGCTGGGG 
TATCCCTGGG TTGAATACTG GGAATTTCTG GGATGTTTTG TTGATCTGTC TTCCCAGGAA 
GGCTTGCAAA GACTAGAAGA ATACCTTATC CAGAAGGAGT TAAGCAAAAA GGCTCAACAA 
GAAATACGAG AAAATGAAGG CTGCCTTCAG GACAGAACCT CAGATTTTGG CAGTGGAAAG 
AAGTACAGCA ACTCCATCTC CGTGGGAGCA TTTCTAGATG GGGATGATGA CAGTAGTTTG 
GAAGAAATTA AAAACCAGCA AAATACAGTT CCAAGTCAGA GTCAGCCCAC AACTGATAAG 
TTCCAAACCT CTAAAAGTGG TTCCCTTCCC TTGGGACAGA AGGTCGACCC TGGAGAAACC 
TCAGTTGGAA CTTACCCAGA TAAAGGCAGA AATGGGTTTT GTCACCCTCT AAACCACAGG 
ACTGCAGATG GAAGGGGACT AGAGGCCACC AATGGGGAGG AGGCCCTTCC ACCACCTGTT 
TCTGTTTTGA CTCAAGAGTT GAATAAACTG AATTTGCAAA GCCTGGGAGA CAGCCTTCAT 
GAGACACCAG ATAAAAACGG GAAACTAGAG GATGAGGTGC TTCCTTCAAG AAAGGGTGCA 
GCAGACAGTG ACTTGCTGGC ATCTCCTCCT GCCATAGCAA GCCTTGGAAA GAAGCAAGTC 
AGGACCAACA CTGAGGTGTC AGAGGCAATG GCTGAAATGT CTTTGGGCCC TAAGAGCCCC 
CAACTTGGAG TTCAGGCTGG TCTGGAACCT ATACTCTCAT CTGCCACGGT AGATTCCACC 
AAGAGGCTCT TCCTGTCTGG AGAGGAACCA TCAAAACTAG ATCGAGATGT TTTAGCAGCT 
CTTGAGTGTG CGAATATTGA CCCTGGTCTG TACCCAGCAA TCCACAGATG GAAAAGCACT 
GTCATGTGCT ACTCGCCCTC AGACAGACAG AGTTGGCCAA GTCCTGCACT CAAAGGGAAG 
TTCACAACTG AGTTGGTGGA TCTCGATTGC TCTCATAGCT GCTCAGGACG ATGCAGTCCA 
GCTGGTAGCA GCCCCAGTAA GCCTGGCCAC ACCTCCTCCT CTTCTGGCCT GCACAGCCCT 
GGACGCTACA GTCCAGCACA TGGGAGACAC TTCCAAAGAG TGGCACACGT GGCCCGACTT 
GCAGCTCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082198.1
CDS	234..3125
Translation	MLWQRLAVVEWAALAWELLGASVLFIAVRWLVRRLEKRPRDLNRCGTLSSPPSASEAVAAQPGEVTMDAMMARLKLLNPDDLRKEVMKAGLKCGPITSTTRFIFEKKLAQALLEQGGLLTSSLPKPSAVTAMAFIQGTSRTPPSVDGKQTQQACFSEDRDFGYSVGLNPPEEEAVASSVHPVPFSASTRNDNHKAGVTAPKEPLVYYGVCPVYEDGPVRHERIHVYEDKKEALQAAKLIKGSRFKAFRTREDAEKFARGICDYLPSPNKTTPLLSPVKAVPLGGSDGLKDGLCLAESETVNKERANSYKNPRTQDLTAKLRKAVENGEEHTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQASMCQLTLETLENPEFMRLMYPDDNMDMLQKRILYVVDLYLNTPDKVGFDTPLHFACKFGNVDVVNVLSSHPLIVKNRKNKYGKTPEEVICERSQNKSPALKERIREYLMGHYYVPLLRAEDTSPVIGELWSSDQKAEASNTAHCRSSPRDPVMTLRAFVGPLSPSKAEDFRKLWKTPPRKKAGFFHSIRKSDPERGIERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLIQKELSKKAQQEIRENEGCLQDRTSDFGSGKKYSNSISVGAFLDGDDDSSLEEIKNQQNTVPSQSQPTTDKFQTSKSGSLPLGQKVDPGETSVGTYPDKGRNGFCHPLNHRTADGRGLEATNGEEALPPPVSVLTQELNKLNLQSLGDSLHETPDKNGKLEDEVLPSRKGAADSDLLASPPAIASLGKKQVRTNTEVSEAMAEMSLGPKSPQLGVQAGLEPILSSATVDSTKRLFLSGEEPSKLDRDVLAALECANIDPGLYPAIHRWKSTVMCYSPSDRQSWPSPALKGKFTTELVDLDCSHSCSGRCSPAGSSPSKPGHTSSSSGLHSPGRYSPAHGRHFQRVAHVARLAAL

Target ORF information:

RefSeq Version	NM_027922.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat and LEM domain containing 2 (Ankle2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027922.2

ORF Insert Sequence:

ATGCTGTGGC AGCGGCTGGC TGTGGTGGAG TGGGCGGCCC TGGCCTGGGA GCTCCTGGGC 
GCCTCGGTGC TGTTCATCGC CGTGCGCTGG CTGGTCCGCC GGCTAGAGAA GCGGCCGCGG 
GACCTGAACC GTTGCGGGAC GCTCTCCTCT CCGCCCAGCG CGTCTGAAGC GGTGGCCGCA 
CAGCCAGGTG AAGTGACAAT GGATGCCATG ATGGCTCGAC TGAAACTCTT GAATCCAGAT 
GACCTTAGAA AAGAAGTTAT GAAAGCTGGA CTTAAATGTG GACCCATTAC ATCAACAACA 
AGATTTATTT TTGAGAAGAA ATTAGCTCAG GCCTTACTGG AACAAGGTGG ATTGCTGACC 
TCTTCCCTTC CCAAGCCCAG CGCGGTTACT GCCATGGCAT TTATCCAGGG CACCTCAAGG 
ACTCCTCCAT CTGTGGACGG GAAACAAACT CAGCAGGCCT GTTTTTCTGA AGACAGAGAT 
TTTGGTTACA GTGTGGGCTT GAACCCTCCA GAAGAGGAAG CGGTAGCATC CAGTGTCCAC 
CCAGTACCCT TCAGTGCCTC TACCAGGAAT GACAACCACA AGGCTGGAGT GACAGCTCCT 
AAGGAGCCAC TTGTGTACTA TGGAGTGTGT CCAGTATATG AGGATGGCCC TGTGAGACAT 
GAGAGGATCC ATGTTTACGA AGATAAAAAG GAAGCTTTAC AAGCTGCCAA GTTGATCAAA 
GGGTCCCGGT TTAAAGCTTT TCGAACAAGA GAAGATGCTG AGAAATTTGC TAGAGGGATA 
TGTGATTATT TACCTTCTCC CAACAAAACT ACGCCCTTAC TGTCTCCTGT GAAGGCAGTG 
CCCCTCGGTG GCAGTGATGG GCTGAAAGAT GGCTTGTGCC TGGCTGAATC AGAAACAGTA 
AACAAAGAAC GAGCAAACAG TTACAAAAAC CCCCGTACCC AGGACCTCAC GGCCAAGCTG 
CGGAAAGCTG TCGAAAACGG GGAGGAACAC ACCTTTTCTG ATCTTATCTG GAGTAATCCT 
CGATATCTAA TTGGTTCTGG GGACAACCCA ACCATTGTAC AAGAAGGATG TAGGTACAAT 
GTCATGCATG TTGCTGCCAA AGAGAACCAG GCTTCCATGT GCCAGCTGAC ATTAGAGACT 
CTGGAGAACC CTGAGTTTAT GCGTCTGATG TACCCAGATG ACAACATGGA CATGCTACAG 
AAGCGCATCC TCTACGTTGT TGACCTGTAT CTTAATACTC CTGACAAAGT GGGCTTTGAT 
ACACCACTGC ATTTTGCTTG TAAATTTGGA AATGTGGATG TAGTCAATGT GCTTTCTTCA 
CACCCTTTGA TTGTGAAAAA CCGAAAGAAT AAATATGGTA AAACACCTGA AGAAGTAATT 
TGTGAAAGAA GCCAAAACAA ATCTCCAGCA CTGAAAGAAA GGATTAGAGA ATACTTAATG 
GGTCACTACT ATGTGCCACT CCTGAGAGCA GAAGACACTT CTCCTGTCAT TGGGGAGCTG 
TGGTCCTCAG ACCAGAAAGC TGAAGCCTCA AATACTGCCC ACTGTAGAAG CAGCCCCAGA 
GATCCTGTGA TGACACTGAG AGCTTTCGTG GGACCTCTGA GTCCATCCAA AGCAGAAGAT 
TTTCGAAAGC TCTGGAAAAC TCCACCTCGA AAGAAAGCAG GCTTCTTCCA CAGCATCAGA 
AAGTCTGACC CAGAACGAGG CATTGAGAGA GTTGGAAGGG AGCTAGCTCA TGAGCTGGGG 
TATCCCTGGG TTGAATACTG GGAATTTCTG GGATGTTTTG TTGATCTGTC TTCCCAGGAA 
GGCTTGCAAA GACTAGAAGA ATACCTTATC CAGAAGGAGT TAAGCAAAAA GGCTCAACAA 
GAAATACGAG AAAATGAAGG CTGCCTTCAG GACAGAACCT CAGATTTTGG CAGTGGAAAG 
AAGTACAGCA ACTCCATCTC CGTGGGAGCA TTTCTAGATG GGGATGATGA CAGTAGTTTG 
GAAGAAATTA AAAACCAGCA AAATACAGTT CCAAGTCAGA GTCAGCCCAC AACTGATAAG 
TTCCAAACCT CTAAAAGTGG TTCCCTTCCC TTGGGACAGA AGGTCGACCC TGGAGAAACC 
TCAGTTGGAA CTTACCCAGA TAAAGGCAGA AATGGGTTTT GTCACCCTCT AAACCACAGG 
ACTGCAGATG GAAGGGGACT AGAGGCCACC AATGGGGAGG AGGCCCTTCC ACCACCTGTT 
TCTGTTTTGA CTCAAGAGTT GAATAAACTG AATTTGCAAA GCCTGGGAGA CAGCCTTCAT 
GAGACACCAG ATAAAAACGG GAAACTAGAG GATGAGGTGC TTCCTTCAAG AAAGGGTGCA 
GCAGACAGTG ACTTGCTGGC ATCTCCTCCT GCCATAGCAA GCCTTGGAAA GAAGCAAGTC 
AGGACCAACA CTGAGGTGTC AGAGGCAATG GCTGAAATGT CTTTGGGCCC TAAGAGCCCC 
CAACTTGGAG TTCAGGCTGG TCTGGAACCT ATACTCTCAT CTGCCACGGT AGATTCCACC 
AAGAGGCTCT TCCTGTCTGG AGAGGAACCA TCAAAACTAG ATCGAGATGT TTTAGCAGCT 
CTTGAGTGTG CGAATATTGA CCCTGGTCTG TACCCAGCAA TCCACAGATG GAAAAGCACT 
GTCATGTGCT ACTCGCCCTC AGACAGACAG AGTTGGCCAA GTCCTGCACT CAAAGGGAAG 
TTCACAACTG AGTTGGTGGA TCTCGATTGC TCTCATAGCT GCTCAGGACG ATGCAGTCCA 
GCTGGTAGCA GCCCCAGTAA GCCTGGCCAC ACCTCCTCCT CTTCTGGCCT GCACAGCCCT 
GGACGCTACA GTCCAGCACA TGGGAGACAC TTCCAAAGAG TGGCACACGT GGCCCGACTT 
GCAGCTCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zinc finger protein Zfp335 is required for the formation of the na?ve T cell compartment.
eLife3(2014 Oct)
Han BY,Wu S,Foo CS,Horton RM,Jenne CN,Watson SR,Whittle B,Goodnow CC,Cyster JG

Tagging genes with cassette-exchange sites.
Nucleic acids research33(4)e44(2005 Mar)
Cobellis G,Nicolaus G,Iovino M,Romito A,Marra E,Barbarisi M,Sardiello M,Di Giorgio FP,Iovino N,Zollo M,Ballabio A,Cortese R

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Nagase T,Ohara O,Koga H

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Genome research11(9)1553-8(2001 Sep)
Piao Y,Ko NT,Lim MK,Ko MS