Spef1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spef1
Entrez Gene ID	70997
Full Name	sperm flagellar 1
Synonyms	4931426K16Rik,Clamp
General protein information	Preferred Names sperm flagellar 1 Names sperm flagellar protein 1 calponin-homology and microtubule-associated protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 F1\|2

mRNA and Protein(s)

mRNA	Protein	Name
NM_027641.2	NP_081917.1	sperm flagellar protein 1

Pathways from BioSystems

Homologies

	SPEF1	XP_001115387.1
Canis lupus familiaris (dog)	SPEF1	XP_005634885.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008402	XP_317050.4
Bos taurus (cattle)	SPEF1	NP_001029594.1
Mus musculus (house mouse)	Spef1	NP_081917.1
Pan troglodytes (chimpanzee)	SPEF1	XP_001161874.1
Rattus norvegicus (Norway rat)	Spef1	NP_001034113.2
Danio rerio (zebrafish)	zgc:66426	NP_956821.1
Homo sapiens (human)	SPEF1	NP_056232.2
Xenopus tropicalis (tropical clawed frog)	spef1	XP_002936340.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

CLAMP, a novel microtubule-associated protein with EB-type calponin homology.
Dougherty GW, Adler HJ, Rzadzinska A, Gimona M, Tomita Y, Lattig MC, Merritt RC Jr, Kachar B
Cell motility and the cytoskeleton62(3)141-56(2005 Nov)

Spef1, a conserved novel testis protein found in mouse sperm flagella.
Chan SW, Fowler KJ, Choo KH, Kalitsis P
Gene353(2)189-99(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a.k.a. Spef1 (sperm flagella) and CLAMP (CaLponin-homology And Microtubule associated Protein) CLAMP/Spef1 expression also in brain, lung, and inner ear MT association of CLAMP requires the CH domain, which has highest similarity with EB family proteins
Title: CLAMP, a novel microtubule-associated protein with EB-type calponin homology.

demonstrate a specific pattern of Spef1 localisation in the seminiferous epithelium of adult mouse testis
Title: Spef1, a conserved novel testis protein found in mouse sperm flagella.

The following Spef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04454	NM_027641.2 Latest version!	Mus musculus sperm flagellar 1 (Spef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spef1 ( NM_027641.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081917.1 Mus musculus sperm flagellar 1 (Spef1), mRNA.

Price & Availability↑

CloneID	OMu04454
Clone ID Related Accession (Same CDS sequence)	NM_027641.2
Accession Version	NM_027641.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	sperm flagellar protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK047675.1 and AK046964.1. On Sep 16, 2006 this sequence version replaced NM_027641.1. ##Evidence-Data-START## Transcript exon combination :: AK047675.1, SRR1660815.208702.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGAAAGCA GCGTGGATGA GGAGGCTCTG CACCAGCTGT ACCTCTGGGT AGACAATATC 
CCTCTGTCCA GACCCAAGAG AAACCTCTCC CGGGACTTCA GTGACGGAGT CCTGGTTGCA 
GAGCTTATCA AGTTTTACTT CCCCAAGATG GTGGAGATGC ACAATTATGT CCCTGCCAAC 
TCTCTCCAGC AGAAGCTCAG CAACTGGGGT CACCTGAACA GAAAGGTGCT GAACAAGCTG 
AACTTCTCGG TCCCTGACGA TGTGATGCGG AAGATTGCGC AGTGTTCTCC GGGAGTAGTG 
GAGCTAGTGC TCATTCCACT GAGGCAGCGC CTGGAGGAGC GGCAGAGGCG CCAGAAGCTG 
GGTGTTGGCT CCTTACAGGA GCTGGCTCCT CAGGATAGCA GTGGCTACAT GGATATGGGT 
TTGCCCCAGA AGGTTCGAGG AGAGGGTGCC CCAGCCTTGG GAGAGCAGCT CAGAGAGGGC 
CGTCCGTTGG CTTCCCGACC CCCTGGGTAT AACCAGGCGC TGCAGGGTGA TCCGAGCTTC 
GTCCTCCAGA TCGCTGAAAA GGAGCAGGAG CTGTTGGCCT CTCAAGAGAC AGTACAGGTC 
CTTCAGATGA AGGTGAAGCG TCTGGAACAT CTGCTCCAAC TTAAGAACGT GCGCATCGAT 
GATCTCTCCC GGCGGCTCCA GCAGGCCGAG CGCAAGCAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081917.1
CDS	138..842
Translation	MESSVDEEALHQLYLWVDNIPLSRPKRNLSRDFSDGVLVAELIKFYFPKMVEMHNYVPANSLQQKLSNWGHLNRKVLNKLNFSVPDDVMRKIAQCSPGVVELVLIPLRQRLEERQRRQKLGVGSLQELAPQDSSGYMDMGLPQKVRGEGAPALGEQLREGRPLASRPPGYNQALQGDPSFVLQIAEKEQELLASQETVQVLQMKVKRLEHLLQLKNVRIDDLSRRLQQAERKQR

Target ORF information:

RefSeq Version	NM_027641.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm flagellar 1 (Spef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027641.2

ORF Insert Sequence:

ATGGAAAGCA GCGTGGATGA GGAGGCTCTG CACCAGCTGT ACCTCTGGGT AGACAATATC 
CCTCTGTCCA GACCCAAGAG AAACCTCTCC CGGGACTTCA GTGACGGAGT CCTGGTTGCA 
GAGCTTATCA AGTTTTACTT CCCCAAGATG GTGGAGATGC ACAATTATGT CCCTGCCAAC 
TCTCTCCAGC AGAAGCTCAG CAACTGGGGT CACCTGAACA GAAAGGTGCT GAACAAGCTG 
AACTTCTCGG TCCCTGACGA TGTGATGCGG AAGATTGCGC AGTGTTCTCC GGGAGTAGTG 
GAGCTAGTGC TCATTCCACT GAGGCAGCGC CTGGAGGAGC GGCAGAGGCG CCAGAAGCTG 
GGTGTTGGCT CCTTACAGGA GCTGGCTCCT CAGGATAGCA GTGGCTACAT GGATATGGGT 
TTGCCCCAGA AGGTTCGAGG AGAGGGTGCC CCAGCCTTGG GAGAGCAGCT CAGAGAGGGC 
CGTCCGTTGG CTTCCCGACC CCCTGGGTAT AACCAGGCGC TGCAGGGTGA TCCGAGCTTC 
GTCCTCCAGA TCGCTGAAAA GGAGCAGGAG CTGTTGGCCT CTCAAGAGAC AGTACAGGTC 
CTTCAGATGA AGGTGAAGCG TCTGGAACAT CTGCTCCAAC TTAAGAACGT GCGCATCGAT 
GATCTCTCCC GGCGGCTCCA GCAGGCCGAG CGCAAGCAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

CLAMP, a novel microtubule-associated protein with EB-type calponin homology.
Cell motility and the cytoskeleton62(3)141-56(2005 Nov)
Dougherty GW,Adler HJ,Rzadzinska A,Gimona M,Tomita Y,Lattig MC,Merritt RC Jr,Kachar B

Spef1, a conserved novel testis protein found in mouse sperm flagella.
Gene353(2)189-99(2005 Jul)
Chan SW,Fowler KJ,Choo KH,Kalitsis P