Slc52a3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc52a3
Entrez Gene ID	69698
Full Name	solute carrier protein family 52, member 3
Synonyms	2310046K01Rik,RFT2
General protein information	Preferred Names solute carrier protein family 52, member 3 Names solute carrier family 52, riboflavin transporter, member 3 riboflavin transporter 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 G3

mRNA and Protein(s)

mRNA	Protein	Name
NM_027172.3	NP_081448.2	solute carrier family 52, riboflavin transporter, member 3 isoform 1 precursor
NM_001164820.1	NP_001158292.1	solute carrier family 52, riboflavin transporter, member 3 isoform 2
NM_001164819.1	NP_001158291.1	solute carrier family 52, riboflavin transporter, member 3 isoform 1 precursor

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000181	XP_310954.5
Xenopus tropicalis (tropical clawed frog)	LOC100486825	XP_002944475.1
Caenorhabditis elegans (roundworm)	Y47D7A.16	NP_001033515.2
Homo sapiens (human)	SLC52A3	NP_212134.3
Pan troglodytes (chimpanzee)	SLC52A3	XP_514458.3
	C20orf54	XP_001112427.1
Mus musculus (house mouse)	Slc52a3	NP_081448.2
Rattus norvegicus (Norway rat)	Slc52a3	NP_001032275.1
Caenorhabditis elegans (roundworm)	Y47D7A.14	NP_001256040.1
Canis lupus familiaris (dog)	SLC52A3	XP_005634955.1
Bos taurus (cattle)	SLC52A3	NP_001014864.1
Danio rerio (zebrafish)	slc52a3	NP_001035447.1
Drosophila melanogaster (fruit fly)	CG11576	NP_651901.3
Gallus gallus (chicken)	C20H20ORF54	XP_417443.3

Gene Ontology
Bibliography

Related articles in PubMed

Disruption of Slc52a3 gene causes neonatal lethality with riboflavin deficiency in mice.
Yoshimatsu H, Yonezawa A, Yamanishi K, Yao Y, Sugano K, Nakagawa S, Imai S, Omura T, Nakagawa T, Yano I, Masuda S, Inui K, Matsubara K
Scientific reports627557(2016 Jun)

SLC52A3, A Brown-Vialetto-van Laere syndrome candidate gene is essential for mouse development, but dispensable for motor neuron differentiation.
Intoh A, Suzuki N, Koszka K, Eggan K
Human molecular genetics25(9)1814-23(2016 05)

Conditional (intestinal-specific) knockout of the riboflavin transporter-3 (RFVT-3) impairs riboflavin absorption.
Subramanian VS, Lambrecht N, Lytle C, Said HM
American journal of physiology. Gastrointestinal and liver physiology310(4)G285-93(2016 Feb)

Identification of anchor genes during kidney development defines ontological relationships, molecular subcompartments and regulatory pathways.
Thiagarajan RD, Georgas KM, Rumballe BA, Lesieur E, Chiu HS, Taylor D, Tang DT, Grimmond SM, Little MH
PloS one6(2)e17286(2011 Feb)

Analysis of HSF4 binding regions reveals its necessity for gene regulation during development and heat shock response in mouse lenses.
Fujimoto M, Oshima K, Shinkawa T, Wang BB, Inouye S, Hayashida N, Takii R, Nakai A
The Journal of biological chemistry283(44)29961-70(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of Slc52a3 gene is associated with Brown-Vialetto-van Laere syndrome.
Title: SLC52A3, A Brown-Vialetto-van Laere syndrome candidate gene is essential for mouse development, but dispensable for motor neuron differentiation.

the RFVT-3 system is the main transporter involved in carrier-mediated riboflavin uptake in the native mouse small and large intestine
Title: Conditional (intestinal-specific) knockout of the riboflavin transporter-3 (RFVT-3) impairs riboflavin absorption.

The following Slc52a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc52a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15335	NM_001164820.1 Latest version!	Mus musculus solute carrier protein family 52, member 3 (Slc52a3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OMu15302	NM_027172.3 Latest version!	Mus musculus solute carrier protein family 52, member 3 (Slc52a3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$272.30 $389.00
OMu15302	NM_001164819.1 Latest version!	Mus musculus solute carrier protein family 52, member 3 (Slc52a3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$272.30 $389.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc52a3 ( NM_001164820.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001158292.1 Mus musculus solute carrier protein family 52, member 3 (Slc52a3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu15335
Clone ID Related Accession (Same CDS sequence)	NM_001164820.1
Accession Version	NM_001164820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	solute carrier family 52, riboflavin transporter, member 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK047618.1, AK029091.1 and CF424499.1. Transcript Variant: This variant (3) has an alternate 5' UTR exon and lacks an internal coding exon, as compared to variant 1. The reading frame is affected, and the resulting isoform (2) is shorter and has a distinct C-terminus, as compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC016127.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTTCC TGACACACCT ACTGGTCTGC GTCTTTGGCA TGGGCTCCTG GGTGGCCATC 
AATGGGCTCT GGGTGGAGCT GCCCCTGCTG GTGACTGAGT TGCCCGAGGC CTGGTACCTG 
CCTTCTTACC TCACTGTGGT CATCCAGCTG GCCAACATAG GCCCCCTCCT GGTCACCCTG 
ATGCACCGCT TCCGACCCGG CTGCCTGTCT GAGGTGCCTG TGATCTTCCT TATCCTGTGC 
GTAGGCACGG CTGCCTGCAT TCTCCTAGCC TTCCTGTGGA ACGTGACCTC CTGGATACAG 
GGTGGACAGC ATAGTGTGGC CTTCATCGTC CTCACCTTCT TCCTGGCCCT GGTGGACTGC 
ACCTCTTCTG TCACCTTCTT GCCCTTCATG AGCCAGCTGC CTACGTACTA TCTTACCACC 
TTCTTCATAG GCGAGGGGCT CAGTGGCCTC CTGCCTGCCC TGGTGGCCCT TGTCCAAGGC 
TCCGGTATCA CCACCTGTGT CAATGTCACG GAGACACCAG GGACCACCTT GAACACTATG 
GAGACTCCCA TCACTCAGGT CGCTGTTATT CCTGGGGGTG CTCACAGTGT TGGGGACCGG 
CTTTGGGGCC TACAATATGG CCATGGCTGC TATGAGCCCC TGCCCTGTCC TGCAGGGTCA 
CTGGGGTGGA GAAGTCCTTA TCGTGCTCTC CTGGGTGCTG TTTGCAGCCT GTCTCAGCTA 
TGTCAAGGTG ATGCTGGGTG TGATCTTGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158292.1
CDS	143..895
Translation	MAFLTHLLVCVFGMGSWVAINGLWVELPLLVTELPEAWYLPSYLTVVIQLANIGPLLVTLMHRFRPGCLSEVPVIFLILCVGTAACILLAFLWNVTSWIQGGQHSVAFIVLTFFLALVDCTSSVTFLPFMSQLPTYYLTTFFIGEGLSGLLPALVALVQGSGITTCVNVTETPGTTLNTMETPITQVAVIPGGAHSVGDRLWGLQYGHGCYEPLPCPAGSLGWRSPYRALLGAVCSLSQLCQGDAGCDLA

Target ORF information:

RefSeq Version	NM_001164820.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier protein family 52, member 3 (Slc52a3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164820.1

ORF Insert Sequence:

ATGGCCTTCC TGACACACCT ACTGGTCTGC GTCTTTGGCA TGGGCTCCTG GGTGGCCATC 
AATGGGCTCT GGGTGGAGCT GCCCCTGCTG GTGACTGAGT TGCCCGAGGC CTGGTACCTG 
CCTTCTTACC TCACTGTGGT CATCCAGCTG GCCAACATAG GCCCCCTCCT GGTCACCCTG 
ATGCACCGCT TCCGACCCGG CTGCCTGTCT GAGGTGCCTG TGATCTTCCT TATCCTGTGC 
GTAGGCACGG CTGCCTGCAT TCTCCTAGCC TTCCTGTGGA ACGTGACCTC CTGGATACAG 
GGTGGACAGC ATAGTGTGGC CTTCATCGTC CTCACCTTCT TCCTGGCCCT GGTGGACTGC 
ACCTCTTCTG TCACCTTCTT GCCCTTCATG AGCCAGCTGC CTACGTACTA TCTTACCACC 
TTCTTCATAG GCGAGGGGCT CAGTGGCCTC CTGCCTGCCC TGGTGGCCCT TGTCCAAGGC 
TCCGGTATCA CCACCTGTGT CAATGTCACG GAGACACCAG GGACCACCTT GAACACTATG 
GAGACTCCCA TCACTCAGGT CGCTGTTATT CCTGGGGGTG CTCACAGTGT TGGGGACCGG 
CTTTGGGGCC TACAATATGG CCATGGCTGC TATGAGCCCC TGCCCTGTCC TGCAGGGTCA 
CTGGGGTGGA GAAGTCCTTA TCGTGCTCTC CTGGGTGCTG TTTGCAGCCT GTCTCAGCTA 
TGTCAAGGTG ATGCTGGGTG TGATCTTGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc52a3 ( NM_027172.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081448.2 Mus musculus solute carrier protein family 52, member 3 (Slc52a3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15302
Clone ID Related Accession (Same CDS sequence)	NM_027172.3 , NM_001164819.1
Accession Version	NM_027172.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	solute carrier family 52, riboflavin transporter, member 3 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ066913.1, AK029091.1 and CF424499.1. On Sep 19, 2009 this sequence version replaced NM_027172.2. Transcript Variant: This variant (1) and variant 2 encode the same protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AK154970.1, AK009850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTTCC TGACACACCT ACTGGTCTGC GTCTTTGGCA TGGGCTCCTG GGTGGCCATC 
AATGGGCTCT GGGTGGAGCT GCCCCTGCTG GTGACTGAGT TGCCCGAGGC CTGGTACCTG 
CCTTCTTACC TCACTGTGGT CATCCAGCTG GCCAACATAG GCCCCCTCCT GGTCACCCTG 
ATGCACCGCT TCCGACCCGG CTGCCTGTCT GAGGTGCCTG TGATCTTCCT TATCCTGTGC 
GTAGGCACGG CTGCCTGCAT TCTCCTAGCC TTCCTGTGGA ACGTGACCTC CTGGATACAG 
GGTGGACAGC ATAGTGTGGC CTTCATCGTC CTCACCTTCT TCCTGGCCCT GGTGGACTGC 
ACCTCTTCTG TCACCTTCTT GCCCTTCATG AGCCAGCTGC CTACGTACTA TCTTACCACC 
TTCTTCATAG GCGAGGGGCT CAGTGGCCTC CTGCCTGCCC TGGTGGCCCT TGTCCAAGGC 
TCCGGTATCA CCACCTGTGT CAATGTCACG GAGACACCAG GGACCACCTT GAACACTATG 
GAGACTCCCA TCACTCAGGG AAACCTTAGC CCTTCCCTGC CGTCGCCCAG CTGGCACCAG 
GAGAGCCGCT ACCTGGCCCC TCGCTTCTCG CCACTGCTCT TCTTCCTACT GCTGTCTTTC 
CTGACAGGCT GCTGTCTGGT GGCCTTCTTC CTCCTGCAGA GGCAGCCCTG GGGACGGCAA 
GGCTCCATAG AGGACCTCCT CCACTCCCAG GTCACCCTGC ACTCTATCAG GCCCCGAGAC 
ACAGAGGACA CCAGCTCCCT GGGTGCCCCT GTGAGCAGCC CAGGCAAGGG ATCAGTGGAA 
GCCAGTGTGG CTTCGCTCCG CCCAGCCCAG CTGGCCTTCA TCTACTCCGT GGTGGCCTTT 
GTCAATGCAC TCACCAATGG CGTTCTGCCC TCCGTGCAGA CCTACTCCTG CCTGCCTTAT 
GGGCCTGTTG CCTACCACCT GTCTGCCACC CTCAGCTCCG TGGCCAGCCC CCTCGCCTGC 
TTCCTCCCCA TCTTCCTGCC TAACAGGTCG CTGTTATTCC TGGGGGTGCT CACAGTGTTG 
GGGACCGGCT TTGGGGCCTA CAATATGGCC ATGGCTGCTA TGAGCCCCTG CCCTGTCCTG 
CAGGGTCACT GGGGTGGAGA AGTCCTTATC GTGCTCTCCT GGGTGCTGTT TGCAGCCTGT 
CTCAGCTATG TCAAGGTGAT GCTGGGTGTG ATCTTGCGTG ACCGGAGTCG CAGTGCCCTC 
TTGTGGTGTG GGGCAGCGGT GCAGCTGGGC TCTCTGATTG GTGCCCTGCT CATGTTCCCA 
CTGGTTAATG TACTGAAACT CTTCTCATCT GCCGACTACT GCAGCCTGGA CTGCTCCGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081448.2
CDS	211..1593
Translation	MAFLTHLLVCVFGMGSWVAINGLWVELPLLVTELPEAWYLPSYLTVVIQLANIGPLLVTLMHRFRPGCLSEVPVIFLILCVGTAACILLAFLWNVTSWIQGGQHSVAFIVLTFFLALVDCTSSVTFLPFMSQLPTYYLTTFFIGEGLSGLLPALVALVQGSGITTCVNVTETPGTTLNTMETPITQGNLSPSLPSPSWHQESRYLAPRFSPLLFFLLLSFLTGCCLVAFFLLQRQPWGRQGSIEDLLHSQVTLHSIRPRDTEDTSSLGAPVSSPGKGSVEASVASLRPAQLAFIYSVVAFVNALTNGVLPSVQTYSCLPYGPVAYHLSATLSSVASPLACFLPIFLPNRSLLFLGVLTVLGTGFGAYNMAMAAMSPCPVLQGHWGGEVLIVLSWVLFAACLSYVKVMLGVILRDRSRSALLWCGAAVQLGSLIGALLMFPLVNVLKLFSSADYCSLDCSV

Target ORF information:

RefSeq Version	NM_027172.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier protein family 52, member 3 (Slc52a3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027172.3

ORF Insert Sequence:

ATGGCCTTCC TGACACACCT ACTGGTCTGC GTCTTTGGCA TGGGCTCCTG GGTGGCCATC 
AATGGGCTCT GGGTGGAGCT GCCCCTGCTG GTGACTGAGT TGCCCGAGGC CTGGTACCTG 
CCTTCTTACC TCACTGTGGT CATCCAGCTG GCCAACATAG GCCCCCTCCT GGTCACCCTG 
ATGCACCGCT TCCGACCCGG CTGCCTGTCT GAGGTGCCTG TGATCTTCCT TATCCTGTGC 
GTAGGCACGG CTGCCTGCAT TCTCCTAGCC TTCCTGTGGA ACGTGACCTC CTGGATACAG 
GGTGGACAGC ATAGTGTGGC CTTCATCGTC CTCACCTTCT TCCTGGCCCT GGTGGACTGC 
ACCTCTTCTG TCACCTTCTT GCCCTTCATG AGCCAGCTGC CTACGTACTA TCTTACCACC 
TTCTTCATAG GCGAGGGGCT CAGTGGCCTC CTGCCTGCCC TGGTGGCCCT TGTCCAAGGC 
TCCGGTATCA CCACCTGTGT CAATGTCACG GAGACACCAG GGACCACCTT GAACACTATG 
GAGACTCCCA TCACTCAGGG AAACCTTAGC CCTTCCCTGC CGTCGCCCAG CTGGCACCAG 
GAGAGCCGCT ACCTGGCCCC TCGCTTCTCG CCACTGCTCT TCTTCCTACT GCTGTCTTTC 
CTGACAGGCT GCTGTCTGGT GGCCTTCTTC CTCCTGCAGA GGCAGCCCTG GGGACGGCAA 
GGCTCCATAG AGGACCTCCT CCACTCCCAG GTCACCCTGC ACTCTATCAG GCCCCGAGAC 
ACAGAGGACA CCAGCTCCCT GGGTGCCCCT GTGAGCAGCC CAGGCAAGGG ATCAGTGGAA 
GCCAGTGTGG CTTCGCTCCG CCCAGCCCAG CTGGCCTTCA TCTACTCCGT GGTGGCCTTT 
GTCAATGCAC TCACCAATGG CGTTCTGCCC TCCGTGCAGA CCTACTCCTG CCTGCCTTAT 
GGGCCTGTTG CCTACCACCT GTCTGCCACC CTCAGCTCCG TGGCCAGCCC CCTCGCCTGC 
TTCCTCCCCA TCTTCCTGCC TAACAGGTCG CTGTTATTCC TGGGGGTGCT CACAGTGTTG 
GGGACCGGCT TTGGGGCCTA CAATATGGCC ATGGCTGCTA TGAGCCCCTG CCCTGTCCTG 
CAGGGTCACT GGGGTGGAGA AGTCCTTATC GTGCTCTCCT GGGTGCTGTT TGCAGCCTGT 
CTCAGCTATG TCAAGGTGAT GCTGGGTGTG ATCTTGCGTG ACCGGAGTCG CAGTGCCCTC 
TTGTGGTGTG GGGCAGCGGT GCAGCTGGGC TCTCTGATTG GTGCCCTGCT CATGTTCCCA 
CTGGTTAATG TACTGAAACT CTTCTCATCT GCCGACTACT GCAGCCTGGA CTGCTCCGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc52a3 ( NM_001164819.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001158291.1 Mus musculus solute carrier protein family 52, member 3 (Slc52a3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15302
Clone ID Related Accession (Same CDS sequence)	NM_027172.3 , NM_001164819.1
Accession Version	NM_001164819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	solute carrier family 52, riboflavin transporter, member 3 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK047618.1, AK029091.1 and CF424499.1. Transcript Variant: This variant (2) has an alternate 5' UTR exon and encodes the same isoform, as compared to variant 1. ##Evidence-Data-START## Transcript exon combination :: AK047618.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTTCC TGACACACCT ACTGGTCTGC GTCTTTGGCA TGGGCTCCTG GGTGGCCATC 
AATGGGCTCT GGGTGGAGCT GCCCCTGCTG GTGACTGAGT TGCCCGAGGC CTGGTACCTG 
CCTTCTTACC TCACTGTGGT CATCCAGCTG GCCAACATAG GCCCCCTCCT GGTCACCCTG 
ATGCACCGCT TCCGACCCGG CTGCCTGTCT GAGGTGCCTG TGATCTTCCT TATCCTGTGC 
GTAGGCACGG CTGCCTGCAT TCTCCTAGCC TTCCTGTGGA ACGTGACCTC CTGGATACAG 
GGTGGACAGC ATAGTGTGGC CTTCATCGTC CTCACCTTCT TCCTGGCCCT GGTGGACTGC 
ACCTCTTCTG TCACCTTCTT GCCCTTCATG AGCCAGCTGC CTACGTACTA TCTTACCACC 
TTCTTCATAG GCGAGGGGCT CAGTGGCCTC CTGCCTGCCC TGGTGGCCCT TGTCCAAGGC 
TCCGGTATCA CCACCTGTGT CAATGTCACG GAGACACCAG GGACCACCTT GAACACTATG 
GAGACTCCCA TCACTCAGGG AAACCTTAGC CCTTCCCTGC CGTCGCCCAG CTGGCACCAG 
GAGAGCCGCT ACCTGGCCCC TCGCTTCTCG CCACTGCTCT TCTTCCTACT GCTGTCTTTC 
CTGACAGGCT GCTGTCTGGT GGCCTTCTTC CTCCTGCAGA GGCAGCCCTG GGGACGGCAA 
GGCTCCATAG AGGACCTCCT CCACTCCCAG GTCACCCTGC ACTCTATCAG GCCCCGAGAC 
ACAGAGGACA CCAGCTCCCT GGGTGCCCCT GTGAGCAGCC CAGGCAAGGG ATCAGTGGAA 
GCCAGTGTGG CTTCGCTCCG CCCAGCCCAG CTGGCCTTCA TCTACTCCGT GGTGGCCTTT 
GTCAATGCAC TCACCAATGG CGTTCTGCCC TCCGTGCAGA CCTACTCCTG CCTGCCTTAT 
GGGCCTGTTG CCTACCACCT GTCTGCCACC CTCAGCTCCG TGGCCAGCCC CCTCGCCTGC 
TTCCTCCCCA TCTTCCTGCC TAACAGGTCG CTGTTATTCC TGGGGGTGCT CACAGTGTTG 
GGGACCGGCT TTGGGGCCTA CAATATGGCC ATGGCTGCTA TGAGCCCCTG CCCTGTCCTG 
CAGGGTCACT GGGGTGGAGA AGTCCTTATC GTGCTCTCCT GGGTGCTGTT TGCAGCCTGT 
CTCAGCTATG TCAAGGTGAT GCTGGGTGTG ATCTTGCGTG ACCGGAGTCG CAGTGCCCTC 
TTGTGGTGTG GGGCAGCGGT GCAGCTGGGC TCTCTGATTG GTGCCCTGCT CATGTTCCCA 
CTGGTTAATG TACTGAAACT CTTCTCATCT GCCGACTACT GCAGCCTGGA CTGCTCCGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158291.1
CDS	143..1525
Translation	MAFLTHLLVCVFGMGSWVAINGLWVELPLLVTELPEAWYLPSYLTVVIQLANIGPLLVTLMHRFRPGCLSEVPVIFLILCVGTAACILLAFLWNVTSWIQGGQHSVAFIVLTFFLALVDCTSSVTFLPFMSQLPTYYLTTFFIGEGLSGLLPALVALVQGSGITTCVNVTETPGTTLNTMETPITQGNLSPSLPSPSWHQESRYLAPRFSPLLFFLLLSFLTGCCLVAFFLLQRQPWGRQGSIEDLLHSQVTLHSIRPRDTEDTSSLGAPVSSPGKGSVEASVASLRPAQLAFIYSVVAFVNALTNGVLPSVQTYSCLPYGPVAYHLSATLSSVASPLACFLPIFLPNRSLLFLGVLTVLGTGFGAYNMAMAAMSPCPVLQGHWGGEVLIVLSWVLFAACLSYVKVMLGVILRDRSRSALLWCGAAVQLGSLIGALLMFPLVNVLKLFSSADYCSLDCSV

Target ORF information:

RefSeq Version	NM_001164819.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier protein family 52, member 3 (Slc52a3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164819.1

ORF Insert Sequence:

ATGGCCTTCC TGACACACCT ACTGGTCTGC GTCTTTGGCA TGGGCTCCTG GGTGGCCATC 
AATGGGCTCT GGGTGGAGCT GCCCCTGCTG GTGACTGAGT TGCCCGAGGC CTGGTACCTG 
CCTTCTTACC TCACTGTGGT CATCCAGCTG GCCAACATAG GCCCCCTCCT GGTCACCCTG 
ATGCACCGCT TCCGACCCGG CTGCCTGTCT GAGGTGCCTG TGATCTTCCT TATCCTGTGC 
GTAGGCACGG CTGCCTGCAT TCTCCTAGCC TTCCTGTGGA ACGTGACCTC CTGGATACAG 
GGTGGACAGC ATAGTGTGGC CTTCATCGTC CTCACCTTCT TCCTGGCCCT GGTGGACTGC 
ACCTCTTCTG TCACCTTCTT GCCCTTCATG AGCCAGCTGC CTACGTACTA TCTTACCACC 
TTCTTCATAG GCGAGGGGCT CAGTGGCCTC CTGCCTGCCC TGGTGGCCCT TGTCCAAGGC 
TCCGGTATCA CCACCTGTGT CAATGTCACG GAGACACCAG GGACCACCTT GAACACTATG 
GAGACTCCCA TCACTCAGGG AAACCTTAGC CCTTCCCTGC CGTCGCCCAG CTGGCACCAG 
GAGAGCCGCT ACCTGGCCCC TCGCTTCTCG CCACTGCTCT TCTTCCTACT GCTGTCTTTC 
CTGACAGGCT GCTGTCTGGT GGCCTTCTTC CTCCTGCAGA GGCAGCCCTG GGGACGGCAA 
GGCTCCATAG AGGACCTCCT CCACTCCCAG GTCACCCTGC ACTCTATCAG GCCCCGAGAC 
ACAGAGGACA CCAGCTCCCT GGGTGCCCCT GTGAGCAGCC CAGGCAAGGG ATCAGTGGAA 
GCCAGTGTGG CTTCGCTCCG CCCAGCCCAG CTGGCCTTCA TCTACTCCGT GGTGGCCTTT 
GTCAATGCAC TCACCAATGG CGTTCTGCCC TCCGTGCAGA CCTACTCCTG CCTGCCTTAT 
GGGCCTGTTG CCTACCACCT GTCTGCCACC CTCAGCTCCG TGGCCAGCCC CCTCGCCTGC 
TTCCTCCCCA TCTTCCTGCC TAACAGGTCG CTGTTATTCC TGGGGGTGCT CACAGTGTTG 
GGGACCGGCT TTGGGGCCTA CAATATGGCC ATGGCTGCTA TGAGCCCCTG CCCTGTCCTG 
CAGGGTCACT GGGGTGGAGA AGTCCTTATC GTGCTCTCCT GGGTGCTGTT TGCAGCCTGT 
CTCAGCTATG TCAAGGTGAT GCTGGGTGTG ATCTTGCGTG ACCGGAGTCG CAGTGCCCTC 
TTGTGGTGTG GGGCAGCGGT GCAGCTGGGC TCTCTGATTG GTGCCCTGCT CATGTTCCCA 
CTGGTTAATG TACTGAAACT CTTCTCATCT GCCGACTACT GCAGCCTGGA CTGCTCCGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Disruption of Slc52a3 gene causes neonatal lethality with riboflavin deficiency in mice.
Scientific reports627557(2016 Jun)
Yoshimatsu H,Yonezawa A,Yamanishi K,Yao Y,Sugano K,Nakagawa S,Imai S,Omura T,Nakagawa T,Yano I,Masuda S,Inui K,Matsubara K

SLC52A3, A Brown-Vialetto-van Laere syndrome candidate gene is essential for mouse development, but dispensable for motor neuron differentiation.
Human molecular genetics25(9)1814-23(2016 05)
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