Polr2d cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polr2d
Entrez Gene ID	69241
Full Name	polymerase (RNA) II (DNA directed) polypeptide D
Synonyms	2310002G05Rik,2610028L19Rik,HSRBP4,RBP4
General protein information	Preferred Names polymerase (RNA) II (DNA directed) polypeptide D Names DNA-directed RNA polymerase II subunit RPB4 DNA-directed RNA polymerase II subunit D RNA polymerase II subunit B4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017317977.1	XP_017173466.1	DNA-directed RNA polymerase II subunit RPB4 isoform X1
NM_027101.2	NP_081377.1	DNA-directed RNA polymerase II subunit RPB4 isoform 2
NM_001357480.1	NP_001344409.1	DNA-directed RNA polymerase II subunit RPB4 isoform 3
NM_027002.3	NP_081278.1	DNA-directed RNA polymerase II subunit RPB4 isoform 1
NM_027002.4	NP_081278.1	DNA-directed RNA polymerase II subunit RPB4 isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Polr2d	NP_081278.1
Gallus gallus (chicken)	POLR2D	NP_001264338.1
Bos taurus (cattle)	POLR2D	NP_001069926.1
Drosophila melanogaster (fruit fly)	Rpb4	NP_001014633.2
Caenorhabditis elegans (roundworm)	rpb-4	NP_495544.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001228	XP_321930.4
Arabidopsis thaliana (thale cress)	NRPB4	NP_196554.1
Oryza sativa (rice)	Os02g0116900	NP_001045683.1
Rattus norvegicus (Norway rat)	Polr2d	NP_001102356.1
Xenopus tropicalis (tropical clawed frog)	polr2d	NP_001016248.1
Homo sapiens (human)	POLR2D	NP_004796.1
Pan troglodytes (chimpanzee)	POLR2D	NP_001238888.1
Canis lupus familiaris (dog)	POLR2D	XP_540983.2
Danio rerio (zebrafish)	polr2d	NP_001002317.1

Gene Ontology
Bibliography

Related articles in PubMed

BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing factor in oocytes.
Yu C, Ji SY, Sha QQ, Dang Y, Zhou JJ, Zhang YL, Liu Y, Wang ZW, Hu B, Sun QY, Sun SC, Tang F, Fan HY
Nature structural & molecular biology23(5)387-94(2016 05)

A conditional knockout resource for the genome-wide study of mouse gene function.
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A
Nature474(7351)337-42(2011 Jun)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Large-scale gene trapping in C57BL/6N mouse embryonic stem cells.
Hansen GM, Markesich DC, Burnett MB, Zhu Q, Dionne KM, Richter LJ, Finnell RH, Sands AT, Zambrowicz BP, Abuin A
Genome research18(10)1670-9(2008 Oct)

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
Nishimura M, Yokoi N, Miki T, Horikawa Y, Yoshioka H, Takeda J, Ohara O, Seino S
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Polr2d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr2d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03403	NM_027002.4 Latest version!	Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu03612	NM_027101.2 Latest version!	Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu79836	NM_001357480.1 Latest version!	Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu79836	XM_017317977.1 Latest version!	Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu03403	NM_027002.3	Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr2d ( NM_027002.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081278.1 Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu03403
Clone ID Related Accession (Same CDS sequence)	NM_027002.3 , NM_027002.4
Accession Version	NM_027002.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	DNA-directed RNA polymerase II subunit RPB4 isoform 1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC161511.3. On Apr 30, 2007 this sequence version replaced NM_027002.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK011600.1, AK009093.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGG GTGGAAGCGA TCCTCGGGCT GGCGACGTAG AAGAAGACGC TTCCCAGCTT 
ATCTTTCCCA AAGAGTTTGA GACAGCGGAG ACTCTGCTGA ACTCGGAAGT CCACATGCTT 
CTGGAGCATC GAAAGCAGCA GAACGAGAGC GCGGAGGACG AGCAGGAGCT GTCGGAGGTC 
TTCATGAAAA CCCTCAACTA CACGGCCCGC TTCAGCCGGT TCAAAAACAG AGAGACCATT 
GCCAGTGTGC GCAGCTTGCT TCTCCAGAAA AAGTTACATA AGTTTGAGTT GGCCTGTTTA 
GCCAATCTTT GCCCGGAGAC TGCTGAAGAG TCCAAAGCTC TGATTCCAAG CCTGGAAGGG 
CGTTTTGAAG ATGAGGAGCT GCAGCAGATT CTCGATGACA TCCAGACGAA GCGCAGCTTC 
CAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081278.1
CDS	14..442
Translation	MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY

Target ORF information:

RefSeq Version	NM_027002.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027002.3

ORF Insert Sequence:

ATGGCGGCGG GTGGAAGCGA TCCTCGGGCT GGCGACGTAG AAGAAGACGC TTCCCAGCTT 
ATCTTTCCCA AAGAGTTTGA GACAGCGGAG ACTCTGCTGA ACTCGGAAGT CCACATGCTT 
CTGGAGCATC GAAAGCAGCA GAACGAGAGC GCGGAGGACG AGCAGGAGCT GTCGGAGGTC 
TTCATGAAAA CCCTCAACTA CACGGCCCGC TTCAGCCGGT TCAAAAACAG AGAGACCATT 
GCCAGTGTGC GCAGCTTGCT TCTCCAGAAA AAGTTACATA AGTTTGAGTT GGCCTGTTTA 
GCCAATCTTT GCCCGGAGAC TGCTGAAGAG TCCAAAGCTC TGATTCCAAG CCTGGAAGGG 
CGTTTTGAAG ATGAGGAGCT GCAGCAGATT CTCGATGACA TCCAGACGAA GCGCAGCTTC 
CAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polr2d ( NM_027002.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081278.1 Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu03403
Clone ID Related Accession (Same CDS sequence)	NM_027002.3 , NM_027002.4
Accession Version	NM_027002.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase II subunit RPB4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161511.3, BU696372.1, AK011600.1 and BP770158.1. On or before Oct 28, 2017 this sequence version replaced NM_027101.2, NM_027002.3. ##Evidence-Data-START## Transcript exon combination :: BC004810.1, AK009093.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG GTGGAAGCGA TCCTCGGGCT GGCGACGTAG AAGAAGACGC TTCCCAGCTT 
ATCTTTCCCA AAGAGTTTGA GACAGCGGAG ACTCTGCTGA ACTCGGAAGT CCACATGCTT 
CTGGAGCATC GAAAGCAGCA GAACGAGAGC GCGGAGGACG AGCAGGAGCT GTCGGAGGTC 
TTCATGAAAA CCCTCAACTA CACGGCCCGC TTCAGCCGGT TCAAAAACAG AGAGACCATT 
GCCAGTGTGC GCAGCTTGCT TCTCCAGAAA AAGTTACATA AGTTTGAGTT GGCCTGTTTA 
GCCAATCTTT GCCCGGAGAC TGCTGAAGAG TCCAAAGCTC TGATTCCAAG CCTGGAAGGG 
CGTTTTGAAG ATGAGGAGCT GCAGCAGATT CTCGATGACA TCCAGACGAA GCGCAGCTTC 
CAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081278.1
CDS	48..476
Translation	MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY

Target ORF information:

RefSeq Version	NM_027002.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027002.4

ORF Insert Sequence:

ATGGCGGCGG GTGGAAGCGA TCCTCGGGCT GGCGACGTAG AAGAAGACGC TTCCCAGCTT 
ATCTTTCCCA AAGAGTTTGA GACAGCGGAG ACTCTGCTGA ACTCGGAAGT CCACATGCTT 
CTGGAGCATC GAAAGCAGCA GAACGAGAGC GCGGAGGACG AGCAGGAGCT GTCGGAGGTC 
TTCATGAAAA CCCTCAACTA CACGGCCCGC TTCAGCCGGT TCAAAAACAG AGAGACCATT 
GCCAGTGTGC GCAGCTTGCT TCTCCAGAAA AAGTTACATA AGTTTGAGTT GGCCTGTTTA 
GCCAATCTTT GCCCGGAGAC TGCTGAAGAG TCCAAAGCTC TGATTCCAAG CCTGGAAGGG 
CGTTTTGAAG ATGAGGAGCT GCAGCAGATT CTCGATGACA TCCAGACGAA GCGCAGCTTC 
CAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polr2d ( NM_027101.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081377.1 Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu03612
Clone ID Related Accession (Same CDS sequence)	NM_027101.2
Accession Version	NM_027101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	DNA-directed RNA polymerase II subunit RPB4 isoform 2
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC161511.3. On Nov 17, 2006 this sequence version replaced NM_027101.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC004810.1, AK009093.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTTCTGG AGCATCGAAA GCAGCAGAAC GAGAGCGCGG AGGACGAGCA GGAGCTGTCG 
GAGGTCTTCA TGAAAACCCT CAACTACACG GCCCGCTTCA GCCGGTTCAA AAACAGAGAG 
ACCATTGCCA GTGTGCGCAG CTTGCTTCTC CAGAAAAAGT TACATAAGTT TGAGTTGGCC 
TGTTTAGCCA ATCTTTGCCC GGAGACTGCT GAAGAGTCCA AAGCTCTGAT TCCAAGCCTG 
GAAGGGCGTT TTGAAGATGA GGAGCTGCAG CAGATTCTCG ATGACATCCA GACGAAGCGC 
AGCTTCCAGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081377.1
CDS	111..425
Translation	MLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY

Target ORF information:

RefSeq Version	NM_027101.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027101.2

ORF Insert Sequence:

ATGCTTCTGG AGCATCGAAA GCAGCAGAAC GAGAGCGCGG AGGACGAGCA GGAGCTGTCG 
GAGGTCTTCA TGAAAACCCT CAACTACACG GCCCGCTTCA GCCGGTTCAA AAACAGAGAG 
ACCATTGCCA GTGTGCGCAG CTTGCTTCTC CAGAAAAAGT TACATAAGTT TGAGTTGGCC 
TGTTTAGCCA ATCTTTGCCC GGAGACTGCT GAAGAGTCCA AAGCTCTGAT TCCAAGCCTG 
GAAGGGCGTT TTGAAGATGA GGAGCTGCAG CAGATTCTCG ATGACATCCA GACGAAGCGC 
AGCTTCCAGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polr2d ( NM_001357480.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344409.1 Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu79836
Clone ID Related Accession (Same CDS sequence)	XM_017317977.1 , NM_001357480.1
Accession Version	NM_001357480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase II subunit RPB4 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161511.3, BU696372.1, BM946715.1 and BP770158.1. On Oct 28, 2017 this sequence version replaced XM_017317977.1. ##Evidence-Data-START## Transcript exon combination :: BM946715.1, BM945733.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG GTGGAAGCGA TCCTCGGGCT GGCGACGTAG AAGAAGACGC TTCCCAGCTT 
ATCTTTCCCA AAGAGTTTGA GACAGCGGAG ACTCTGCTGA ACTCGGAAGT CCACATGCTT 
CTGGAGCATC GAAAGCAGCA GAACGAGAGC GCGGAGGACG AGCAGGAGCT GTCGGAGGTC 
TTCATGAAAA CCCTCAACTA CACGGCCCGC TTCAGCCGGT TCAAAAACAG AGAGACCATT 
GCCAGTGTGC GCAGCCTGGA AGGGCGTTTT GAAGATGAGG AGCTGCAGCA GATTCTCGAT 
GACATCCAGA CGAAGCGCAG CTTCCAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344409.1
CDS	48..380
Translation	MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLEGRFEDEELQQILDDIQTKRSFQY

Target ORF information:

RefSeq Version	NM_001357480.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357480.1

ORF Insert Sequence:

ATGGCGGCGG GTGGAAGCGA TCCTCGGGCT GGCGACGTAG AAGAAGACGC TTCCCAGCTT 
ATCTTTCCCA AAGAGTTTGA GACAGCGGAG ACTCTGCTGA ACTCGGAAGT CCACATGCTT 
CTGGAGCATC GAAAGCAGCA GAACGAGAGC GCGGAGGACG AGCAGGAGCT GTCGGAGGTC 
TTCATGAAAA CCCTCAACTA CACGGCCCGC TTCAGCCGGT TCAAAAACAG AGAGACCATT 
GCCAGTGTGC GCAGCCTGGA AGGGCGTTTT GAAGATGAGG AGCTGCAGCA GATTCTCGAT 
GACATCCAGA CGAAGCGCAG CTTCCAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polr2d ( XM_017317977.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173466.1 Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu79836
Clone ID Related Accession (Same CDS sequence)	XM_017317977.1 , NM_001357480.1
Accession Version	XM_017317977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	DNA-directed RNA polymerase II subunit RPB4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GTGGAAGCGA TCCTCGGGCT GGCGACGTAG AAGAAGACGC TTCCCAGCTT 
ATCTTTCCCA AAGAGTTTGA GACAGCGGAG ACTCTGCTGA ACTCGGAAGT CCACATGCTT 
CTGGAGCATC GAAAGCAGCA GAACGAGAGC GCGGAGGACG AGCAGGAGCT GTCGGAGGTC 
TTCATGAAAA CCCTCAACTA CACGGCCCGC TTCAGCCGGT TCAAAAACAG AGAGACCATT 
GCCAGTGTGC GCAGCCTGGA AGGGCGTTTT GAAGATGAGG AGCTGCAGCA GATTCTCGAT 
GACATCCAGA CGAAGCGCAG CTTCCAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173466.1
CDS	65..397
Translation	MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLEGRFEDEELQQILDDIQTKRSFQY

Target ORF information:

RefSeq Version	XM_017317977.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus polymerase (RNA) II (DNA directed) polypeptide D (Polr2d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317977.1

ORF Insert Sequence:

ATGGCGGCGG GTGGAAGCGA TCCTCGGGCT GGCGACGTAG AAGAAGACGC TTCCCAGCTT 
ATCTTTCCCA AAGAGTTTGA GACAGCGGAG ACTCTGCTGA ACTCGGAAGT CCACATGCTT 
CTGGAGCATC GAAAGCAGCA GAACGAGAGC GCGGAGGACG AGCAGGAGCT GTCGGAGGTC 
TTCATGAAAA CCCTCAACTA CACGGCCCGC TTCAGCCGGT TCAAAAACAG AGAGACCATT 
GCCAGTGTGC GCAGCCTGGA AGGGCGTTTT GAAGATGAGG AGCTGCAGCA GATTCTCGAT 
GACATCCAGA CGAAGCGCAG CTTCCAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing factor in oocytes.
Nature structural & molecular biology23(5)387-94(2016 05)
Yu C,Ji SY,Sha QQ,Dang Y,Zhou JJ,Zhang YL,Liu Y,Wang ZW,Hu B,Sun QY,Sun SC,Tang F,Fan HY

A conditional knockout resource for the genome-wide study of mouse gene function.
Nature474(7351)337-42(2011 Jun)
Skarnes WC,Rosen B,West AP,Koutsourakis M,Bushell W,Iyer V,Mujica AO,Thomas M,Harrow J,Cox T,Jackson D,Severin J,Biggs P,Fu J,Nefedov M,de Jong PJ,Stewart AF,Bradley A

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Large-scale gene trapping in C57BL/6N mouse embryonic stem cells.
Genome research18(10)1670-9(2008 Oct)
Hansen GM,Markesich DC,Burnett MB,Zhu Q,Dionne KM,Richter LJ,Finnell RH,Sands AT,Zambrowicz BP,Abuin A

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)
Nishimura M,Yokoi N,Miki T,Horikawa Y,Yoshioka H,Takeda J,Ohara O,Seino S

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Polr2d cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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