Fitm1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fitm1
Entrez Gene ID	68680
Full Name	fat storage-inducing transmembrane protein 1
Synonyms	1110028A07Rik,AV010547,Cg10671,Fit1
General protein information	Preferred Names fat storage-inducing transmembrane protein 1 Names fat storage-inducing transmembrane protein 1 fat-inducing protein 1 fat-inducing transcript 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_026808.1	NP_081084.1	fat storage-inducing transmembrane protein 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC792443	XP_001331953.2
Mus musculus (house mouse)	Fitm1	NP_081084.1
Danio rerio (zebrafish)	fitm1	NP_001013343.1
Pan troglodytes (chimpanzee)	FITM1	XP_522807.2
Bos taurus (cattle)	FITM1	NP_001098821.1
Xenopus tropicalis (tropical clawed frog)	fitm1	XP_004910817.1
Homo sapiens (human)	FITM1	NP_981947.1
	FITM1	XP_001111682.1
Canis lupus familiaris (dog)	FITM1	XP_850360.1
Rattus norvegicus (Norway rat)	Fitm1	NP_001099507.1

Gene Ontology
Bibliography

Related articles in PubMed

Direct binding of triglyceride to fat storage-inducing transmembrane proteins 1 and 2 is important for lipid droplet formation.
Gross DA, Zhan C, Silver DL
Proceedings of the National Academy of Sciences of the United States of America108(49)19581-6(2011 Dec)

Structural insights into triglyceride storage mediated by fat storage-inducing transmembrane (FIT) protein 2.
Gross DA, Snapp EL, Silver DL
PloS one5(5)e10796(2010 May)

Evolutionarily conserved gene family important for fat storage.
Kadereit B, Kumar P, Wang WJ, Miranda D, Snapp EL, Severina N, Torregroza I, Evans T, Silver DL
Proceedings of the National Academy of Sciences of the United States of America105(1)94-9(2008 Jan)

Nucleotide sequence analysis of the approximately 35-kb segment containing interferon-gamma-inducible mouse proteasome activator genes.
Yawata M, Murata S, Tanaka K, Ishigatsubo Y, Kasahara M
Immunogenetics53(2)119-29(2001 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings indicate that lipid droplet (LD)produced by storage-inducing transmembrane proteins 2 (FIT2) were approximately 80% larger than storage-inducing transmembrane proteins 1 (FIT1).
Title: Direct binding of triglyceride to fat storage-inducing transmembrane proteins 1 and 2 is important for lipid droplet formation.

FIT1 and FIT2 are endoplasmic reticulum resident membrane proteins that induce lipid droplet accumulation in cell culture and when expressed in mouse liver.
Title: Evolutionarily conserved gene family important for fat storage.

The following Fitm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fitm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03068	NM_026808.1 Latest version!	Mus musculus fat storage-inducing transmembrane protein 1 (Fitm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fitm1 ( NM_026808.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081084.1 Mus musculus fat storage-inducing transmembrane protein 1 (Fitm1), mRNA.

Price & Availability↑

CloneID	OMu03068
Clone ID Related Accession (Same CDS sequence)	NM_026808.1
Accession Version	NM_026808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	fat storage-inducing transmembrane protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB054000.1. ##Evidence-Data-START## Transcript exon combination :: BC104408.1, AB054000.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAACGGG GGCCGACGGT GGGGGCAGGG CTGGGGGCCG GGACCCGAGT CCGAGCACTG 
CTGGGATGCC TGGTCAAGGT GCTGCTCTGG GTGGCCTCTG CCTTACTGTA CTTTGGAAGT 
GAACAAGCCG CCCGCCTCCT GGGCAGCCCT TGCTTACGGC GCCTCTACCA TGCTTGGTTG 
GCAGCAGTGG TCATCTTTGG GCCCCTTCTG CAGTTTCACG TTAACTCTCG GACGATCTTC 
GCTAGCCACG GCAACTTCTT CAACATAAAG TTTGTGAATT CAGCATGGGG CTGGACATGC 
ACCTTCCTGG GGGGCTTTGT GCTGCTGGTG GTGTTCCTGG CTACACGACG TGTAGCAGTG 
ACTGCCAGGC ACCTGAGCCG ACTGGTGGTG GGGGCAGCTG TATGGCGGGG GGCCGGACGT 
GCCTTCCTAC TCATCGAGGA CCTGACGGGT TCCTGCTTCG AGCCTCTGCC CCAGGGCCTA 
TTGCTGCATG AGCTGCCCGA CCGCAAGAGT TGCCTGGCAG CCGGCCACCA GTGGAGGGGC 
TACACTGTCT CCTCCCACAC CTTCCTCCTC ACCTTCTGCT GCCTCCTCAT GGCTGAGGAA 
GCCGCTGTGT TTGCCAAGTA CCTGGCCCAT GGGCTACCAG CTGGCGCCCC ACTGCGTCTT 
GTTTTCCTAC TCAATGTGCT GCTGCTGGGC CTCTGGAACT TCCTGCTGCT CTGCACGGTC 
ATCTATTTCC ATCAATATAC CCACAAGGTG GTGGGTGCGG CAGTAGGCAC GTTTGCCTGG 
TACCTTACCT ATGGCAGCTG GTACCATCAA CCCTGGTCCC CTGGGATCCC AGGCCACGGG 
CTCTTCCCTC GATCCCGCTC AATGCGCAAA CATAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081084.1
CDS	9..887
Translation	MERGPTVGAGLGAGTRVRALLGCLVKVLLWVASALLYFGSEQAARLLGSPCLRRLYHAWLAAVVIFGPLLQFHVNSRTIFASHGNFFNIKFVNSAWGWTCTFLGGFVLLVVFLATRRVAVTARHLSRLVVGAAVWRGAGRAFLLIEDLTGSCFEPLPQGLLLHELPDRKSCLAAGHQWRGYTVSSHTFLLTFCCLLMAEEAAVFAKYLAHGLPAGAPLRLVFLLNVLLLGLWNFLLLCTVIYFHQYTHKVVGAAVGTFAWYLTYGSWYHQPWSPGIPGHGLFPRSRSMRKHN

Target ORF information:

RefSeq Version	NM_026808.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus fat storage-inducing transmembrane protein 1 (Fitm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026808.1

ORF Insert Sequence:

ATGGAACGGG GGCCGACGGT GGGGGCAGGG CTGGGGGCCG GGACCCGAGT CCGAGCACTG 
CTGGGATGCC TGGTCAAGGT GCTGCTCTGG GTGGCCTCTG CCTTACTGTA CTTTGGAAGT 
GAACAAGCCG CCCGCCTCCT GGGCAGCCCT TGCTTACGGC GCCTCTACCA TGCTTGGTTG 
GCAGCAGTGG TCATCTTTGG GCCCCTTCTG CAGTTTCACG TTAACTCTCG GACGATCTTC 
GCTAGCCACG GCAACTTCTT CAACATAAAG TTTGTGAATT CAGCATGGGG CTGGACATGC 
ACCTTCCTGG GGGGCTTTGT GCTGCTGGTG GTGTTCCTGG CTACACGACG TGTAGCAGTG 
ACTGCCAGGC ACCTGAGCCG ACTGGTGGTG GGGGCAGCTG TATGGCGGGG GGCCGGACGT 
GCCTTCCTAC TCATCGAGGA CCTGACGGGT TCCTGCTTCG AGCCTCTGCC CCAGGGCCTA 
TTGCTGCATG AGCTGCCCGA CCGCAAGAGT TGCCTGGCAG CCGGCCACCA GTGGAGGGGC 
TACACTGTCT CCTCCCACAC CTTCCTCCTC ACCTTCTGCT GCCTCCTCAT GGCTGAGGAA 
GCCGCTGTGT TTGCCAAGTA CCTGGCCCAT GGGCTACCAG CTGGCGCCCC ACTGCGTCTT 
GTTTTCCTAC TCAATGTGCT GCTGCTGGGC CTCTGGAACT TCCTGCTGCT CTGCACGGTC 
ATCTATTTCC ATCAATATAC CCACAAGGTG GTGGGTGCGG CAGTAGGCAC GTTTGCCTGG 
TACCTTACCT ATGGCAGCTG GTACCATCAA CCCTGGTCCC CTGGGATCCC AGGCCACGGG 
CTCTTCCCTC GATCCCGCTC AATGCGCAAA CATAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Direct binding of triglyceride to fat storage-inducing transmembrane proteins 1 and 2 is important for lipid droplet formation.
Proceedings of the National Academy of Sciences of the United States of America108(49)19581-6(2011 Dec)
Gross DA,Zhan C,Silver DL

Structural insights into triglyceride storage mediated by fat storage-inducing transmembrane (FIT) protein 2.
PloS one5(5)e10796(2010 May)
Gross DA,Snapp EL,Silver DL

Evolutionarily conserved gene family important for fat storage.
Proceedings of the National Academy of Sciences of the United States of America105(1)94-9(2008 Jan)
Kadereit B,Kumar P,Wang WJ,Miranda D,Snapp EL,Severina N,Torregroza I,Evans T,Silver DL

Nucleotide sequence analysis of the approximately 35-kb segment containing interferon-gamma-inducible mouse proteasome activator genes.
Immunogenetics53(2)119-29(2001 Mar)
Yawata M,Murata S,Tanaka K,Ishigatsubo Y,Kasahara M