Mdp1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mdp1
Entrez Gene ID	67881
Full Name	magnesium-dependent phosphatase 1
Synonyms	1810034K20Rik,AI035604
General protein information	Preferred Names magnesium-dependent phosphatase 1 Names magnesium-dependent phosphatase 1 MDP-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_023397.4	NP_075886.1	magnesium-dependent phosphatase 1
NM_023397.5	NP_075886.1	magnesium-dependent phosphatase 1
XM_017316173.1	XP_017171662.1	magnesium-dependent phosphatase 1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mdp1	NP_075886.1
Danio rerio (zebrafish)	si:dkeyp-27c8.2	XP_685841.1
Magnaporthe oryzae (rice blast fungus)	MGG_04948	XP_003712443.1
Canis lupus familiaris (dog)	MDP1	XP_537388.3
Neurospora crassa	NCU07321	XP_962479.1
Homo sapiens (human)	MDP1	NP_612485.2
Rattus norvegicus (Norway rat)	Mdp1	NP_001099509.1
Arabidopsis thaliana (thale cress)	AT2G14110	NP_179027.2
Bos taurus (cattle)	MDP-1	NP_001157693.1
Schizosaccharomyces pombe (fission yeast)	SPBP8B7.31	NP_596538.2

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

X-ray crystal structure of the hypothetical phosphotyrosine phosphatase MDP-1 of the haloacid dehalogenase superfamily.
Peisach E, Selengut JD, Dunaway-Mariano D, Allen KN
Biochemistry43(40)12770-9(2004 Oct)

MDP-1 is a new and distinct member of the haloacid dehalogenase family of aspartate-dependent phosphohydrolases.
Selengut JD
Biochemistry40(42)12704-11(2001 Oct)

MDP-1: A novel eukaryotic magnesium-dependent phosphatase.
Selengut JD, Levine RL
Biochemistry39(28)8315-24(2000 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

X ray crystallography of MDP-1
Title: X-ray crystal structure of the hypothetical phosphotyrosine phosphatase MDP-1 of the haloacid dehalogenase superfamily.

The following Mdp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mdp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02264	NM_023397.5 Latest version!	Mus musculus magnesium-dependent phosphatase 1 (Mdp1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu78843	XM_017316173.1 Latest version!	Mus musculus magnesium-dependent phosphatase 1 (Mdp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu02264	NM_023397.4	Mus musculus magnesium-dependent phosphatase 1 (Mdp1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mdp1 ( NM_023397.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075886.1 Mus musculus magnesium-dependent phosphatase 1 (Mdp1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02264
Clone ID Related Accession (Same CDS sequence)	NM_023397.4 , NM_023397.5
Accession Version	NM_023397.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-30
Organism	Mus musculus(house mouse)
Product	magnesium-dependent phosphatase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ176407.1, BC046613.1 and CR519902.1. On Aug 15, 2009 this sequence version replaced NM_023397.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: BC046613.1, BQ963162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGCGGC TGCCAAAGCT TGCGGTTTTT GATCTGGATT ACACGCTCTG GCCTTTCTGG 
GTAGATACAC ACGTAGACCC CCCATTCCAC AAGAGCAGCG ATGGAACTGT ACGAGACAGG 
CGGGGCCAGA ACATCCAATT GTACCCGGAG GTGCCCGAGG TCTTGGGAAG ACTGCAGAGC 
CTTGGCGTGC CTGTCGCCGC TGCTTCTCGG ACAAGTGAGA TACAAGGGGC CAACCAACTC 
CTGGAGCTCT TTGACCTTGG CAAATACTTT ATTCAGCGGG AAATCTACCC AGGCAGCAAA 
GTCACACACT TTGAGAGGTT GCACCACAAG ACCGGAGTTC CTTTCTCTCA GATGGTCTTC 
TTTGATGATG AGAATCGGAA TATCATAGAC GTTGGCAGAC TTGGCGTTAC CTGCATTCAC 
ATCCGGGATG GGATGAGTCT TCAAACGTTA ACTCAAGGAT TAGAGACATT TGCAAAGGCC 
CAAGCTGGAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075886.1
CDS	135..629
Translation	MTRLPKLAVFDLDYTLWPFWVDTHVDPPFHKSSDGTVRDRRGQNIQLYPEVPEVLGRLQSLGVPVAAASRTSEIQGANQLLELFDLGKYFIQREIYPGSKVTHFERLHHKTGVPFSQMVFFDDENRNIIDVGRLGVTCIHIRDGMSLQTLTQGLETFAKAQAGL

Target ORF information:

RefSeq Version	NM_023397.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus magnesium-dependent phosphatase 1 (Mdp1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023397.4

ORF Insert Sequence:

ATGACGCGGC TGCCAAAGCT TGCGGTTTTT GATCTGGATT ACACGCTCTG GCCTTTCTGG 
GTAGATACAC ACGTAGACCC CCCATTCCAC AAGAGCAGCG ATGGAACTGT ACGAGACAGG 
CGGGGCCAGA ACATCCAATT GTACCCGGAG GTGCCCGAGG TCTTGGGAAG ACTGCAGAGC 
CTTGGCGTGC CTGTCGCCGC TGCTTCTCGG ACAAGTGAGA TACAAGGGGC CAACCAACTC 
CTGGAGCTCT TTGACCTTGG CAAATACTTT ATTCAGCGGG AAATCTACCC AGGCAGCAAA 
GTCACACACT TTGAGAGGTT GCACCACAAG ACCGGAGTTC CTTTCTCTCA GATGGTCTTC 
TTTGATGATG AGAATCGGAA TATCATAGAC GTTGGCAGAC TTGGCGTTAC CTGCATTCAC 
ATCCGGGATG GGATGAGTCT TCAAACGTTA ACTCAAGGAT TAGAGACATT TGCAAAGGCC 
CAAGCTGGAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdp1 ( NM_023397.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075886.1 Mus musculus magnesium-dependent phosphatase 1 (Mdp1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02264
Clone ID Related Accession (Same CDS sequence)	NM_023397.4 , NM_023397.5
Accession Version	NM_023397.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	magnesium-dependent phosphatase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK007319.1, BC046613.2 and AI846874.1. On Jul 31, 2018 this sequence version replaced NM_023397.4. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.1955054.1, SRR7345562.2655985.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGCGGC TGCCAAAGCT TGCGGTTTTT GATCTGGATT ACACGCTCTG GCCTTTCTGG 
GTAGATACAC ACGTAGACCC CCCATTCCAC AAGAGCAGCG ATGGAACTGT ACGAGACAGG 
CGGGGCCAGA ACATCCAATT GTACCCGGAG GTGCCCGAGG TCTTGGGAAG ACTGCAGAGC 
CTTGGCGTGC CTGTCGCCGC TGCTTCTCGG ACAAGTGAGA TACAAGGGGC CAACCAACTC 
CTGGAGCTCT TTGACCTTGG CAAATACTTT ATTCAGCGGG AAATCTACCC AGGCAGCAAA 
GTCACACACT TTGAGAGGTT GCACCACAAG ACCGGAGTTC CTTTCTCTCA GATGGTCTTC 
TTTGATGATG AGAATCGGAA TATCATAGAC GTTGGCAGAC TTGGCGTTAC CTGCATTCAC 
ATCCGGGATG GGATGAGTCT TCAAACGTTA ACTCAAGGAT TAGAGACATT TGCAAAGGCC 
CAAGCTGGAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075886.1
CDS	78..572
Translation	MTRLPKLAVFDLDYTLWPFWVDTHVDPPFHKSSDGTVRDRRGQNIQLYPEVPEVLGRLQSLGVPVAAASRTSEIQGANQLLELFDLGKYFIQREIYPGSKVTHFERLHHKTGVPFSQMVFFDDENRNIIDVGRLGVTCIHIRDGMSLQTLTQGLETFAKAQAGL

Target ORF information:

RefSeq Version	NM_023397.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus magnesium-dependent phosphatase 1 (Mdp1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023397.5

ORF Insert Sequence:

ATGACGCGGC TGCCAAAGCT TGCGGTTTTT GATCTGGATT ACACGCTCTG GCCTTTCTGG 
GTAGATACAC ACGTAGACCC CCCATTCCAC AAGAGCAGCG ATGGAACTGT ACGAGACAGG 
CGGGGCCAGA ACATCCAATT GTACCCGGAG GTGCCCGAGG TCTTGGGAAG ACTGCAGAGC 
CTTGGCGTGC CTGTCGCCGC TGCTTCTCGG ACAAGTGAGA TACAAGGGGC CAACCAACTC 
CTGGAGCTCT TTGACCTTGG CAAATACTTT ATTCAGCGGG AAATCTACCC AGGCAGCAAA 
GTCACACACT TTGAGAGGTT GCACCACAAG ACCGGAGTTC CTTTCTCTCA GATGGTCTTC 
TTTGATGATG AGAATCGGAA TATCATAGAC GTTGGCAGAC TTGGCGTTAC CTGCATTCAC 
ATCCGGGATG GGATGAGTCT TCAAACGTTA ACTCAAGGAT TAGAGACATT TGCAAAGGCC 
CAAGCTGGAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdp1 ( XM_017316173.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171662.1 Mus musculus magnesium-dependent phosphatase 1 (Mdp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78843
Clone ID Related Accession (Same CDS sequence)	XM_017316173.1
Accession Version	XM_017316173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	magnesium-dependent phosphatase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCGGC TGCCAAAGCT TGCGGTTTTT GATCTGGATT ACACGCTCTG GCCTTTCTGG 
GTAGATACAC ACGTAGACCC CCCATTCCAC AAGAGCAGCG ATGGAACTGT ACGAGACAGG 
CGGGGCCAGA ACATCCAATT GTACCCGGAG GTGCCCGAGG TCTTGGGAAG ACTGCAGAGC 
CTTGGCGTGC CTGTCGCCGC TGCTTCTCGG ACAAGTGAGA TACAAGGGGC CAACCAACTC 
CTGGAGCTCT TTGACCTTGG CAAATGTTAC CTGCATTCAC ATCCGGGATG GGATGAGTCT 
TCAAACGTTA ACTCAAGGAT TAGAGACATT TGCAAAGGCC CAAGCTGGAC TCTGAGGTCC 
AATCCTGTGG ATAAGCCTCA TCTGCAGTGT AAAACTAAAA TCAAGAAAGC CTTGTCAGGG 
GCTCTTGTAT TAAAGTGTCC ACGTGTGACA GTCAGGGTTC TGCCACCGTA TGGGTTGTTT 
CCATTTTTCT TTTCTTTTTC ACAAAGATTT ATTTATGTAT ATGATACTGT CATTGTCTTC 
AGACACACCA GAAGAGGGTA TCGTATCAGA TCCCATTACA GATGGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171662.1
CDS	167..757
Translation	MTRLPKLAVFDLDYTLWPFWVDTHVDPPFHKSSDGTVRDRRGQNIQLYPEVPEVLGRLQSLGVPVAAASRTSEIQGANQLLELFDLGKCYLHSHPGWDESSNVNSRIRDICKGPSWTLRSNPVDKPHLQCKTKIKKALSGALVLKCPRVTVRVLPPYGLFPFFFSFSQRFIYVYDTVIVFRHTRRGYRIRSHYRWL

Target ORF information:

RefSeq Version	XM_017316173.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus magnesium-dependent phosphatase 1 (Mdp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316173.1

ORF Insert Sequence:

ATGACGCGGC TGCCAAAGCT TGCGGTTTTT GATCTGGATT ACACGCTCTG GCCTTTCTGG 
GTAGATACAC ACGTAGACCC CCCATTCCAC AAGAGCAGCG ATGGAACTGT ACGAGACAGG 
CGGGGCCAGA ACATCCAATT GTACCCGGAG GTGCCCGAGG TCTTGGGAAG ACTGCAGAGC 
CTTGGCGTGC CTGTCGCCGC TGCTTCTCGG ACAAGTGAGA TACAAGGGGC CAACCAACTC 
CTGGAGCTCT TTGACCTTGG CAAATGTTAC CTGCATTCAC ATCCGGGATG GGATGAGTCT 
TCAAACGTTA ACTCAAGGAT TAGAGACATT TGCAAAGGCC CAAGCTGGAC TCTGAGGTCC 
AATCCTGTGG ATAAGCCTCA TCTGCAGTGT AAAACTAAAA TCAAGAAAGC CTTGTCAGGG 
GCTCTTGTAT TAAAGTGTCC ACGTGTGACA GTCAGGGTTC TGCCACCGTA TGGGTTGTTT 
CCATTTTTCT TTTCTTTTTC ACAAAGATTT ATTTATGTAT ATGATACTGT CATTGTCTTC 
AGACACACCA GAAGAGGGTA TCGTATCAGA TCCCATTACA GATGGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

X-ray crystal structure of the hypothetical phosphotyrosine phosphatase MDP-1 of the haloacid dehalogenase superfamily.
Biochemistry43(40)12770-9(2004 Oct)
Peisach E,Selengut JD,Dunaway-Mariano D,Allen KN

MDP-1 is a new and distinct member of the haloacid dehalogenase family of aspartate-dependent phosphohydrolases.
Biochemistry40(42)12704-11(2001 Oct)
Selengut JD

MDP-1: A novel eukaryotic magnesium-dependent phosphatase.
Biochemistry39(28)8315-24(2000 Jul)
Selengut JD,Levine RL