Nelfcd cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nelfcd
Entrez Gene ID	57314
Full Name	negative elongation factor complex member C/D, Th1l
Synonyms	2410003I03Rik,C77797,Th1,Th1l
General protein information	Preferred Names negative elongation factor complex member C/D, Th1l Names negative elongation factor D NELF-D TH1 homolog TH1-like homolog TH1-like protein trihydrophobin 1 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H4

mRNA and Protein(s)

mRNA	Protein	Name
XM_006499946.3	XP_006500009.1	negative elongation factor D isoform X1
XM_006499946.2	XP_006500009.1	negative elongation factor D isoform X1
NM_020580.3	NP_065605.2	negative elongation factor D

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC477276	XP_005635295.1
Danio rerio (zebrafish)	nelfcd	NP_957484.1
Xenopus tropicalis (tropical clawed frog)	nelfcd	NP_001025682.1
Pan troglodytes (chimpanzee)	TH1L	XP_001139660.1
	TH1L	XP_001084700.2
Homo sapiens (human)	NELFCD	NP_945327.2
Gallus gallus (chicken)	TH1L	NP_001006294.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000391	XP_310712.4
Bos taurus (cattle)	LOC514958	XP_002692460.1
Mus musculus (house mouse)	Nelfcd	NP_065605.2
Rattus norvegicus (Norway rat)	Nelfcd	NP_001258183.1
Drosophila melanogaster (fruit fly)	TH1	NP_573123.1

Gene Ontology
Bibliography

Related articles in PubMed

Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.
Pan H, Zhao X, Zhang X, Abouelsoud M, Sun J, April C, Amleh A, Fan JB, Hu Y, Li R
PloS one10(5)e0127422(2015)

Transcription factors expressed in olfactory bulb local progenitor cells revealed by genome-wide transcriptome profiling.
Campbell GR, Baudhuin A, Vranizan K, Ngai J
Molecular and cellular neurosciences46(2)548-61(2011 Feb)

Trihydrophobin 1 attenuates androgen signal transduction through promoting androgen receptor degradation.
Yang Y, Zou W, Kong X, Wang H, Zong H, Jiang J, Wang Y, Hong Y, Chi Y, Xie J, Gu J
Journal of cellular biochemistry109(5)1013-24(2010 Apr)

Nrf2 protects against pulmonary fibrosis by regulating the lung oxidant level and Th1/Th2 balance.
Kikuchi N, Ishii Y, Morishima Y, Yageta Y, Haraguchi N, Itoh K, Yamamoto M, Hizawa N
Respiratory research1131(2010 Mar)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that TH1 is a novel regulator to control the duration and magnitude of androgen signal transduction and might be directly involved in androgen-related developmental, physiological, and pathological processes.
Title: Trihydrophobin 1 attenuates androgen signal transduction through promoting androgen receptor degradation.

results indicated that Nrf2 protects against the development of pulmonary fibrosis by regulating the cellular redox level and lung Th1/Th2 balance.
Title: Nrf2 protects against pulmonary fibrosis by regulating the lung oxidant level and Th1/Th2 balance.

TH1 interacts with PAK1 and specifically restricts the activation of MAPK modules through the upstream region of the MAPK pathway, thereby influencing cell migration.
Title: Trihydrophobin 1 Interacts with PAK1 and Regulates ERK/MAPK Activation and Cell Migration.

In conclusion, our data suggest that RelB is an important target for morphine modulation of proinflammatory and Th1 cytokines. They also indicate that morphine uses multiple intracellular pathways to exert its generalized immunosuppression.
Title: Differential involvement of RelB in morphine-induced modulation of chemotaxis, NO, and cytokine production in murine macrophages and lymphocytes.

The gene expression of TH1 in mouse testis and changes of TH1 protein during postnatal development, were analyzed.
Title: Expression and distribution of trihydrophobin 1 in postnatal developing mouse testis.

The following Nelfcd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nelfcd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12046	NM_020580.3 Latest version!	Mus musculus negative elongation factor complex member C/D, Th1l (Nelfcd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$349.30 $499.00
OMu30734	XM_006499946.3 Latest version!	Mus musculus negative elongation factor complex member C/D, Th1l (Nelfcd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu30734	XM_006499946.2	Mus musculus negative elongation factor complex member C/D, Th1l (Nelfcd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nelfcd ( XM_006499946.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500009.1 Mus musculus negative elongation factor complex member C/D, Th1l (Nelfcd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30734
Clone ID Related Accession (Same CDS sequence)	XM_006499946.3 , XM_006499946.2
Accession Version	XM_006499946.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	negative elongation factor D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499946.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCT CCATCTTCAA CACTCTGAAG AGGTATTTCC AGGCAGGAGG GTCTCCAGAA 
AACGTCATCC AGCTCTTATC TGAGAACTAC ACGGCAGTGG CCCAGACAGT GAACCTGCTG 
GCTGAGTGGC TCATTCAGAC AGGCGTTGAG CCTGTGCAGG TGCAGGAGAC TGTGGAAAAC 
CACTTGAAGA GTTTGCTGAT CAAGCACTTC GACCCTCGGA AAGCAGATTC TATTTTCACT 
GAAGAAGGAG AGACCCCAGC TTGGCTTGAG CAGATGATCG CACACACAAC ATGGAGAGAT 
CTGTTTTATA AGTTGGCCGA GGCCCATCCT GACTGTTTGA TGCTCAACTT CACTGTGAAG 
CTCATTTCTG ATGCGGGGTA CCAAGGGGAG ATCACCAGCG TGTCCACAGC CTGCCAGCAG 
TTGGAAGTGT TCTCCAGAGT GCTTCGGACC TCTCTAGCCA CGATCTTGGA TGGGGGAGAA 
GAAAACCTTG AGAAAAATCT CCCTGAGTTT GCAAAGATGG TATGCCATGG CGAGCACACG 
TACCTGTTTG CACAGGCCAT GATGTCCGTG CTGGCACAGG AGGAACAGGG TGGTTCTGCA 
GTGCGCAGGG TCGCTCAGGA AGTCCAGCGC TTCGCCCAGG AGAAGGGCCA TGATGCCAGT 
CAGATCACGC TGGCCTTGGG CACAGCTGCC TCCTACCCCC GAGCCTGCCA AGCGCTTGGC 
GCCATGCTGT CCAGAGGAGC CCTGAATCCT GCAGACATCA CGGTCTTGTT CAAGATGTTC 
ACCAGCATGG ACCCTCCTCC CGTGGAACTC ATCCGGGTGC CGGCCTTCCT GGACCTGTTC 
ATGCAGTCGC TCTTCAAGCC AGGGGCCAAG ATCAACCAGG ACCACAAACA CAAGTACATC 
CACATCCTGG CTTACGCTGC CAGTGTGGTT GAGACCTGGA AGAAGAACAA GCGGGTCAGC 
ATTGGCAAAG ACGAGCTCAA GTCCACGTCA AAGGCTATCG AGACTGTCCA CAACCTGTGC 
TGCAACGAGA ACAAAGGGGC CTCTGAGCTG GTGGCTGAGC TGAGCACACT TTATCAGTGC 
ATTCGGTTTC CAGTGGTGGC GATGGGCGTG CTCAAGTGGG TAGATTGGAC CGTATCGGAG 
CCAAGGTACT TCCAGCTGCA GACTGATCAC ACTCCAGTTC ACCTGGCCTT GTTGGATGAG 
ATCAGCACCT GCCACCAGCT CCTGCACCCC CAGGTTCTGC AGCTGCTTGT TAAGCTTTTT 
GAGACAGAGC ACTCCCAGCT AGACGTGATG GAGCAGCTCG AGTTGAAAAA GACCCTGCTG 
GACAGGATGG TGCACCTGCT GAGCCGCGGC TACGTCCTGC CGGTGGTCAG TTATATCCGC 
AAATGTCTGG AGAAGCTGGA CACGGACATC TCCCTCATCC GCTACTTTGT CACTGAGGTT 
CTGGATGTCA TCGCCCCTCC GTACACCTCA GACTTTGTGC AGCTTTTCCT CCCCATCTTG 
GAAAATGACA GCATCGCGGG CACCATCAAG GCAGAGGGCG AGCATGACCC CGTGACGGAG 
TTCATTGCTC ACTGCAAGTC GAACTTCATC GTGGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500009.1
CDS	274..1875
Translation	MEPSIFNTLKRYFQAGGSPENVIQLLSENYTAVAQTVNLLAEWLIQTGVEPVQVQETVENHLKSLLIKHFDPRKADSIFTEEGETPAWLEQMIAHTTWRDLFYKLAEAHPDCLMLNFTVKLISDAGYQGEITSVSTACQQLEVFSRVLRTSLATILDGGEENLEKNLPEFAKMVCHGEHTYLFAQAMMSVLAQEEQGGSAVRRVAQEVQRFAQEKGHDASQITLALGTAASYPRACQALGAMLSRGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGAKINQDHKHKYIHILAYAASVVETWKKNKRVSIGKDELKSTSKAIETVHNLCCNENKGASELVAELSTLYQCIRFPVVAMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEISTCHQLLHPQVLQLLVKLFETEHSQLDVMEQLELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKLDTDISLIRYFVTEVLDVIAPPYTSDFVQLFLPILENDSIAGTIKAEGEHDPVTEFIAHCKSNFIVVN

Target ORF information:

RefSeq Version	XM_006499946.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus negative elongation factor complex member C/D, Th1l (Nelfcd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499946.2

ORF Insert Sequence:

ATGGAGCCCT CCATCTTCAA CACTCTGAAG AGGTATTTCC AGGCAGGAGG GTCTCCAGAA 
AACGTCATCC AGCTCTTATC TGAGAACTAC ACGGCAGTGG CCCAGACAGT GAACCTGCTG 
GCTGAGTGGC TCATTCAGAC AGGCGTTGAG CCTGTGCAGG TGCAGGAGAC TGTGGAAAAC 
CACTTGAAGA GTTTGCTGAT CAAGCACTTC GACCCTCGGA AAGCAGATTC TATTTTCACT 
GAAGAAGGAG AGACCCCAGC TTGGCTTGAG CAGATGATCG CACACACAAC ATGGAGAGAT 
CTGTTTTATA AGTTGGCCGA GGCCCATCCT GACTGTTTGA TGCTCAACTT CACTGTGAAG 
CTCATTTCTG ATGCGGGGTA CCAAGGGGAG ATCACCAGCG TGTCCACAGC CTGCCAGCAG 
TTGGAAGTGT TCTCCAGAGT GCTTCGGACC TCTCTAGCCA CGATCTTGGA TGGGGGAGAA 
GAAAACCTTG AGAAAAATCT CCCTGAGTTT GCAAAGATGG TATGCCATGG CGAGCACACG 
TACCTGTTTG CACAGGCCAT GATGTCCGTG CTGGCACAGG AGGAACAGGG TGGTTCTGCA 
GTGCGCAGGG TCGCTCAGGA AGTCCAGCGC TTCGCCCAGG AGAAGGGCCA TGATGCCAGT 
CAGATCACGC TGGCCTTGGG CACAGCTGCC TCCTACCCCC GAGCCTGCCA AGCGCTTGGC 
GCCATGCTGT CCAGAGGAGC CCTGAATCCT GCAGACATCA CGGTCTTGTT CAAGATGTTC 
ACCAGCATGG ACCCTCCTCC CGTGGAACTC ATCCGGGTGC CGGCCTTCCT GGACCTGTTC 
ATGCAGTCGC TCTTCAAGCC AGGGGCCAAG ATCAACCAGG ACCACAAACA CAAGTACATC 
CACATCCTGG CTTACGCTGC CAGTGTGGTT GAGACCTGGA AGAAGAACAA GCGGGTCAGC 
ATTGGCAAAG ACGAGCTCAA GTCCACGTCA AAGGCTATCG AGACTGTCCA CAACCTGTGC 
TGCAACGAGA ACAAAGGGGC CTCTGAGCTG GTGGCTGAGC TGAGCACACT TTATCAGTGC 
ATTCGGTTTC CAGTGGTGGC GATGGGCGTG CTCAAGTGGG TAGATTGGAC CGTATCGGAG 
CCAAGGTACT TCCAGCTGCA GACTGATCAC ACTCCAGTTC ACCTGGCCTT GTTGGATGAG 
ATCAGCACCT GCCACCAGCT CCTGCACCCC CAGGTTCTGC AGCTGCTTGT TAAGCTTTTT 
GAGACAGAGC ACTCCCAGCT AGACGTGATG GAGCAGCTCG AGTTGAAAAA GACCCTGCTG 
GACAGGATGG TGCACCTGCT GAGCCGCGGC TACGTCCTGC CGGTGGTCAG TTATATCCGC 
AAATGTCTGG AGAAGCTGGA CACGGACATC TCCCTCATCC GCTACTTTGT CACTGAGGTT 
CTGGATGTCA TCGCCCCTCC GTACACCTCA GACTTTGTGC AGCTTTTCCT CCCCATCTTG 
GAAAATGACA GCATCGCGGG CACCATCAAG GCAGAGGGCG AGCATGACCC CGTGACGGAG 
TTCATTGCTC ACTGCAAGTC GAACTTCATC GTGGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nelfcd ( NM_020580.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_065605.2 Mus musculus negative elongation factor complex member C/D, Th1l (Nelfcd), mRNA.

Price & Availability↑

CloneID	OMu12046
Clone ID Related Accession (Same CDS sequence)	NM_020580.3
Accession Version	NM_020580.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	negative elongation factor D
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL844534.4 and AK010360.1. On Apr 17, 2007 this sequence version replaced NM_020580.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ238742.1, SRR1660815.11192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGTC CGGCGCCGGG TACAATTATG GGAGAAGACT ACTTCGGGAA TGCCTCAGAG 
TGGGGCGAAG AGGCAGACGG CGGCCAGCAT CAGGAAGATG ATTCCGGAGA AGGAGAGGAC 
GATGCTGAGG TGCAGCAGGA ATGCCTGCAT AAGTTTTCCA CCCGGGACTA CATCATGGAG 
CCCTCCATCT TCAACACTCT GAAGAGGTAT TTCCAGGCAG GAGGGTCTCC AGAAAACGTC 
ATCCAGCTCT TATCTGAGAA CTACACGGCA GTGGCCCAGA CAGTGAACCT GCTGGCTGAG 
TGGCTCATTC AGACAGGCGT TGAGCCTGTG CAGGTGCAGG AGACTGTGGA AAACCACTTG 
AAGAGTTTGC TGATCAAGCA CTTCGACCCT CGGAAAGCAG ATTCTATTTT CACTGAAGAA 
GGAGAGACCC CAGCTTGGCT TGAGCAGATG ATCGCACACA CAACATGGAG AGATCTGTTT 
TATAAGTTGG CCGAGGCCCA TCCTGACTGT TTGATGCTCA ACTTCACTGT GAAGCTCATT 
TCTGATGCGG GGTACCAAGG GGAGATCACC AGCGTGTCCA CAGCCTGCCA GCAGTTGGAA 
GTGTTCTCCA GAGTGCTTCG GACCTCTCTA GCCACGATCT TGGATGGGGG AGAAGAAAAC 
CTTGAGAAAA ATCTCCCTGA GTTTGCAAAG ATGGTATGCC ATGGCGAGCA CACGTACCTG 
TTTGCACAGG CCATGATGTC CGTGCTGGCA CAGGAGGAAC AGGGTGGTTC TGCAGTGCGC 
AGGGTCGCTC AGGAAGTCCA GCGCTTCGCC CAGGAGAAGG GCCATGATGC CAGTCAGATC 
ACGCTGGCCT TGGGCACAGC TGCCTCCTAC CCCCGAGCCT GCCAAGCGCT TGGCGCCATG 
CTGTCCAGAG GAGCCCTGAA TCCTGCAGAC ATCACGGTCT TGTTCAAGAT GTTCACCAGC 
ATGGACCCTC CTCCCGTGGA ACTCATCCGG GTGCCGGCCT TCCTGGACCT GTTCATGCAG 
TCGCTCTTCA AGCCAGGGGC CAAGATCAAC CAGGACCACA AACACAAGTA CATCCACATC 
CTGGCTTACG CTGCCAGTGT GGTTGAGACC TGGAAGAAGA ACAAGCGGGT CAGCATTGGC 
AAAGACGAGC TCAAGTCCAC GTCAAAGGCT ATCGAGACTG TCCACAACCT GTGCTGCAAC 
GAGAACAAAG GGGCCTCTGA GCTGGTGGCT GAGCTGAGCA CACTTTATCA GTGCATTCGG 
TTTCCAGTGG TGGCGATGGG CGTGCTCAAG TGGGTAGATT GGACCGTATC GGAGCCAAGG 
TACTTCCAGC TGCAGACTGA TCACACTCCA GTTCACCTGG CCTTGTTGGA TGAGATCAGC 
ACCTGCCACC AGCTCCTGCA CCCCCAGGTT CTGCAGCTGC TTGTTAAGCT TTTTGAGACA 
GAGCACTCCC AGCTAGACGT GATGGAGCAG CTCGAGTTGA AAAAGACCCT GCTGGACAGG 
ATGGTGCACC TGCTGAGCCG CGGCTACGTC CTGCCGGTGG TCAGTTATAT CCGCAAATGT 
CTGGAGAAGC TGGACACGGA CATCTCCCTC ATCCGCTACT TTGTCACTGA GGTTCTGGAT 
GTCATCGCCC CTCCGTACAC CTCAGACTTT GTGCAGCTTT TCCTCCCCAT CTTGGAAAAT 
GACAGCATCG CGGGCACCAT CAAGGCAGAG GGCGAGCATG ACCCCGTGAC GGAGTTCATT 
GCTCACTGCA AGTCGAACTT CATCGTGGTG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065605.2
CDS	67..1842
Translation	MAGPAPGTIMGEDYFGNASEWGEEADGGQHQEDDSGEGEDDAEVQQECLHKFSTRDYIMEPSIFNTLKRYFQAGGSPENVIQLLSENYTAVAQTVNLLAEWLIQTGVEPVQVQETVENHLKSLLIKHFDPRKADSIFTEEGETPAWLEQMIAHTTWRDLFYKLAEAHPDCLMLNFTVKLISDAGYQGEITSVSTACQQLEVFSRVLRTSLATILDGGEENLEKNLPEFAKMVCHGEHTYLFAQAMMSVLAQEEQGGSAVRRVAQEVQRFAQEKGHDASQITLALGTAASYPRACQALGAMLSRGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGAKINQDHKHKYIHILAYAASVVETWKKNKRVSIGKDELKSTSKAIETVHNLCCNENKGASELVAELSTLYQCIRFPVVAMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEISTCHQLLHPQVLQLLVKLFETEHSQLDVMEQLELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKLDTDISLIRYFVTEVLDVIAPPYTSDFVQLFLPILENDSIAGTIKAEGEHDPVTEFIAHCKSNFIVVN

Target ORF information:

RefSeq Version	NM_020580.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus negative elongation factor complex member C/D, Th1l (Nelfcd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020580.3

ORF Insert Sequence:

ATGGCGGGTC CGGCGCCGGG TACAATTATG GGAGAAGACT ACTTCGGGAA TGCCTCAGAG 
TGGGGCGAAG AGGCAGACGG CGGCCAGCAT CAGGAAGATG ATTCCGGAGA AGGAGAGGAC 
GATGCTGAGG TGCAGCAGGA ATGCCTGCAT AAGTTTTCCA CCCGGGACTA CATCATGGAG 
CCCTCCATCT TCAACACTCT GAAGAGGTAT TTCCAGGCAG GAGGGTCTCC AGAAAACGTC 
ATCCAGCTCT TATCTGAGAA CTACACGGCA GTGGCCCAGA CAGTGAACCT GCTGGCTGAG 
TGGCTCATTC AGACAGGCGT TGAGCCTGTG CAGGTGCAGG AGACTGTGGA AAACCACTTG 
AAGAGTTTGC TGATCAAGCA CTTCGACCCT CGGAAAGCAG ATTCTATTTT CACTGAAGAA 
GGAGAGACCC CAGCTTGGCT TGAGCAGATG ATCGCACACA CAACATGGAG AGATCTGTTT 
TATAAGTTGG CCGAGGCCCA TCCTGACTGT TTGATGCTCA ACTTCACTGT GAAGCTCATT 
TCTGATGCGG GGTACCAAGG GGAGATCACC AGCGTGTCCA CAGCCTGCCA GCAGTTGGAA 
GTGTTCTCCA GAGTGCTTCG GACCTCTCTA GCCACGATCT TGGATGGGGG AGAAGAAAAC 
CTTGAGAAAA ATCTCCCTGA GTTTGCAAAG ATGGTATGCC ATGGCGAGCA CACGTACCTG 
TTTGCACAGG CCATGATGTC CGTGCTGGCA CAGGAGGAAC AGGGTGGTTC TGCAGTGCGC 
AGGGTCGCTC AGGAAGTCCA GCGCTTCGCC CAGGAGAAGG GCCATGATGC CAGTCAGATC 
ACGCTGGCCT TGGGCACAGC TGCCTCCTAC CCCCGAGCCT GCCAAGCGCT TGGCGCCATG 
CTGTCCAGAG GAGCCCTGAA TCCTGCAGAC ATCACGGTCT TGTTCAAGAT GTTCACCAGC 
ATGGACCCTC CTCCCGTGGA ACTCATCCGG GTGCCGGCCT TCCTGGACCT GTTCATGCAG 
TCGCTCTTCA AGCCAGGGGC CAAGATCAAC CAGGACCACA AACACAAGTA CATCCACATC 
CTGGCTTACG CTGCCAGTGT GGTTGAGACC TGGAAGAAGA ACAAGCGGGT CAGCATTGGC 
AAAGACGAGC TCAAGTCCAC GTCAAAGGCT ATCGAGACTG TCCACAACCT GTGCTGCAAC 
GAGAACAAAG GGGCCTCTGA GCTGGTGGCT GAGCTGAGCA CACTTTATCA GTGCATTCGG 
TTTCCAGTGG TGGCGATGGG CGTGCTCAAG TGGGTAGATT GGACCGTATC GGAGCCAAGG 
TACTTCCAGC TGCAGACTGA TCACACTCCA GTTCACCTGG CCTTGTTGGA TGAGATCAGC 
ACCTGCCACC AGCTCCTGCA CCCCCAGGTT CTGCAGCTGC TTGTTAAGCT TTTTGAGACA 
GAGCACTCCC AGCTAGACGT GATGGAGCAG CTCGAGTTGA AAAAGACCCT GCTGGACAGG 
ATGGTGCACC TGCTGAGCCG CGGCTACGTC CTGCCGGTGG TCAGTTATAT CCGCAAATGT 
CTGGAGAAGC TGGACACGGA CATCTCCCTC ATCCGCTACT TTGTCACTGA GGTTCTGGAT 
GTCATCGCCC CTCCGTACAC CTCAGACTTT GTGCAGCTTT TCCTCCCCAT CTTGGAAAAT 
GACAGCATCG CGGGCACCAT CAAGGCAGAG GGCGAGCATG ACCCCGTGAC GGAGTTCATT 
GCTCACTGCA AGTCGAACTT CATCGTGGTG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nelfcd ( XM_006499946.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500009.1 Mus musculus negative elongation factor complex member C/D, Th1l (Nelfcd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30734
Clone ID Related Accession (Same CDS sequence)	XM_006499946.3 , XM_006499946.2
Accession Version	XM_006499946.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	negative elongation factor D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499946.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCT CCATCTTCAA CACTCTGAAG AGGTATTTCC AGGCAGGAGG GTCTCCAGAA 
AACGTCATCC AGCTCTTATC TGAGAACTAC ACGGCAGTGG CCCAGACAGT GAACCTGCTG 
GCTGAGTGGC TCATTCAGAC AGGCGTTGAG CCTGTGCAGG TGCAGGAGAC TGTGGAAAAC 
CACTTGAAGA GTTTGCTGAT CAAGCACTTC GACCCTCGGA AAGCAGATTC TATTTTCACT 
GAAGAAGGAG AGACCCCAGC TTGGCTTGAG CAGATGATCG CACACACAAC ATGGAGAGAT 
CTGTTTTATA AGTTGGCCGA GGCCCATCCT GACTGTTTGA TGCTCAACTT CACTGTGAAG 
CTCATTTCTG ATGCGGGGTA CCAAGGGGAG ATCACCAGCG TGTCCACAGC CTGCCAGCAG 
TTGGAAGTGT TCTCCAGAGT GCTTCGGACC TCTCTAGCCA CGATCTTGGA TGGGGGAGAA 
GAAAACCTTG AGAAAAATCT CCCTGAGTTT GCAAAGATGG TATGCCATGG CGAGCACACG 
TACCTGTTTG CACAGGCCAT GATGTCCGTG CTGGCACAGG AGGAACAGGG TGGTTCTGCA 
GTGCGCAGGG TCGCTCAGGA AGTCCAGCGC TTCGCCCAGG AGAAGGGCCA TGATGCCAGT 
CAGATCACGC TGGCCTTGGG CACAGCTGCC TCCTACCCCC GAGCCTGCCA AGCGCTTGGC 
GCCATGCTGT CCAGAGGAGC CCTGAATCCT GCAGACATCA CGGTCTTGTT CAAGATGTTC 
ACCAGCATGG ACCCTCCTCC CGTGGAACTC ATCCGGGTGC CGGCCTTCCT GGACCTGTTC 
ATGCAGTCGC TCTTCAAGCC AGGGGCCAAG ATCAACCAGG ACCACAAACA CAAGTACATC 
CACATCCTGG CTTACGCTGC CAGTGTGGTT GAGACCTGGA AGAAGAACAA GCGGGTCAGC 
ATTGGCAAAG ACGAGCTCAA GTCCACGTCA AAGGCTATCG AGACTGTCCA CAACCTGTGC 
TGCAACGAGA ACAAAGGGGC CTCTGAGCTG GTGGCTGAGC TGAGCACACT TTATCAGTGC 
ATTCGGTTTC CAGTGGTGGC GATGGGCGTG CTCAAGTGGG TAGATTGGAC CGTATCGGAG 
CCAAGGTACT TCCAGCTGCA GACTGATCAC ACTCCAGTTC ACCTGGCCTT GTTGGATGAG 
ATCAGCACCT GCCACCAGCT CCTGCACCCC CAGGTTCTGC AGCTGCTTGT TAAGCTTTTT 
GAGACAGAGC ACTCCCAGCT AGACGTGATG GAGCAGCTCG AGTTGAAAAA GACCCTGCTG 
GACAGGATGG TGCACCTGCT GAGCCGCGGC TACGTCCTGC CGGTGGTCAG TTATATCCGC 
AAATGTCTGG AGAAGCTGGA CACGGACATC TCCCTCATCC GCTACTTTGT CACTGAGGTT 
CTGGATGTCA TCGCCCCTCC GTACACCTCA GACTTTGTGC AGCTTTTCCT CCCCATCTTG 
GAAAATGACA GCATCGCGGG CACCATCAAG GCAGAGGGCG AGCATGACCC CGTGACGGAG 
TTCATTGCTC ACTGCAAGTC GAACTTCATC GTGGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500009.1
CDS	279..1880
Translation	MEPSIFNTLKRYFQAGGSPENVIQLLSENYTAVAQTVNLLAEWLIQTGVEPVQVQETVENHLKSLLIKHFDPRKADSIFTEEGETPAWLEQMIAHTTWRDLFYKLAEAHPDCLMLNFTVKLISDAGYQGEITSVSTACQQLEVFSRVLRTSLATILDGGEENLEKNLPEFAKMVCHGEHTYLFAQAMMSVLAQEEQGGSAVRRVAQEVQRFAQEKGHDASQITLALGTAASYPRACQALGAMLSRGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGAKINQDHKHKYIHILAYAASVVETWKKNKRVSIGKDELKSTSKAIETVHNLCCNENKGASELVAELSTLYQCIRFPVVAMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEISTCHQLLHPQVLQLLVKLFETEHSQLDVMEQLELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKLDTDISLIRYFVTEVLDVIAPPYTSDFVQLFLPILENDSIAGTIKAEGEHDPVTEFIAHCKSNFIVVN

Target ORF information:

RefSeq Version	XM_006499946.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus negative elongation factor complex member C/D, Th1l (Nelfcd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499946.3

ORF Insert Sequence:

ATGGAGCCCT CCATCTTCAA CACTCTGAAG AGGTATTTCC AGGCAGGAGG GTCTCCAGAA 
AACGTCATCC AGCTCTTATC TGAGAACTAC ACGGCAGTGG CCCAGACAGT GAACCTGCTG 
GCTGAGTGGC TCATTCAGAC AGGCGTTGAG CCTGTGCAGG TGCAGGAGAC TGTGGAAAAC 
CACTTGAAGA GTTTGCTGAT CAAGCACTTC GACCCTCGGA AAGCAGATTC TATTTTCACT 
GAAGAAGGAG AGACCCCAGC TTGGCTTGAG CAGATGATCG CACACACAAC ATGGAGAGAT 
CTGTTTTATA AGTTGGCCGA GGCCCATCCT GACTGTTTGA TGCTCAACTT CACTGTGAAG 
CTCATTTCTG ATGCGGGGTA CCAAGGGGAG ATCACCAGCG TGTCCACAGC CTGCCAGCAG 
TTGGAAGTGT TCTCCAGAGT GCTTCGGACC TCTCTAGCCA CGATCTTGGA TGGGGGAGAA 
GAAAACCTTG AGAAAAATCT CCCTGAGTTT GCAAAGATGG TATGCCATGG CGAGCACACG 
TACCTGTTTG CACAGGCCAT GATGTCCGTG CTGGCACAGG AGGAACAGGG TGGTTCTGCA 
GTGCGCAGGG TCGCTCAGGA AGTCCAGCGC TTCGCCCAGG AGAAGGGCCA TGATGCCAGT 
CAGATCACGC TGGCCTTGGG CACAGCTGCC TCCTACCCCC GAGCCTGCCA AGCGCTTGGC 
GCCATGCTGT CCAGAGGAGC CCTGAATCCT GCAGACATCA CGGTCTTGTT CAAGATGTTC 
ACCAGCATGG ACCCTCCTCC CGTGGAACTC ATCCGGGTGC CGGCCTTCCT GGACCTGTTC 
ATGCAGTCGC TCTTCAAGCC AGGGGCCAAG ATCAACCAGG ACCACAAACA CAAGTACATC 
CACATCCTGG CTTACGCTGC CAGTGTGGTT GAGACCTGGA AGAAGAACAA GCGGGTCAGC 
ATTGGCAAAG ACGAGCTCAA GTCCACGTCA AAGGCTATCG AGACTGTCCA CAACCTGTGC 
TGCAACGAGA ACAAAGGGGC CTCTGAGCTG GTGGCTGAGC TGAGCACACT TTATCAGTGC 
ATTCGGTTTC CAGTGGTGGC GATGGGCGTG CTCAAGTGGG TAGATTGGAC CGTATCGGAG 
CCAAGGTACT TCCAGCTGCA GACTGATCAC ACTCCAGTTC ACCTGGCCTT GTTGGATGAG 
ATCAGCACCT GCCACCAGCT CCTGCACCCC CAGGTTCTGC AGCTGCTTGT TAAGCTTTTT 
GAGACAGAGC ACTCCCAGCT AGACGTGATG GAGCAGCTCG AGTTGAAAAA GACCCTGCTG 
GACAGGATGG TGCACCTGCT GAGCCGCGGC TACGTCCTGC CGGTGGTCAG TTATATCCGC 
AAATGTCTGG AGAAGCTGGA CACGGACATC TCCCTCATCC GCTACTTTGT CACTGAGGTT 
CTGGATGTCA TCGCCCCTCC GTACACCTCA GACTTTGTGC AGCTTTTCCT CCCCATCTTG 
GAAAATGACA GCATCGCGGG CACCATCAAG GCAGAGGGCG AGCATGACCC CGTGACGGAG 
TTCATTGCTC ACTGCAAGTC GAACTTCATC GTGGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.
PloS one10(5)e0127422(2015)
Pan H,Zhao X,Zhang X,Abouelsoud M,Sun J,April C,Amleh A,Fan JB,Hu Y,Li R

Transcription factors expressed in olfactory bulb local progenitor cells revealed by genome-wide transcriptome profiling.
Molecular and cellular neurosciences46(2)548-61(2011 Feb)
Campbell GR,Baudhuin A,Vranizan K,Ngai J

Trihydrophobin 1 attenuates androgen signal transduction through promoting androgen receptor degradation.
Journal of cellular biochemistry109(5)1013-24(2010 Apr)
Yang Y,Zou W,Kong X,Wang H,Zong H,Jiang J,Wang Y,Hong Y,Chi Y,Xie J,Gu J

Nrf2 protects against pulmonary fibrosis by regulating the lung oxidant level and Th1/Th2 balance.
Respiratory research1131(2010 Mar)
Kikuchi N,Ishii Y,Morishima Y,Yageta Y,Haraguchi N,Itoh K,Yamamoto M,Hizawa N

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

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Nelfcd cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム