Brsk1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brsk1
Entrez Gene ID	381979
Full Name	BR serine/threonine kinase 1
Synonyms	Gm1100,SAD-B,SADB
General protein information	Preferred Names BR serine/threonine kinase 1 Names serine/threonine-protein kinase BRSK1 BR serine/threonine-protein kinase 1 brain-specific serine/threonine-protein kinase 1 serine/threonine kinase SAD-B serine/threonine kinase SADB serine/threonine-protein kinase SAD-B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017322326.1	XP_017177815.1	serine/threonine-protein kinase BRSK1 isoform X2
XM_017322325.1	XP_017177814.1	serine/threonine-protein kinase BRSK1 isoform X1
XM_017322326.2	XP_017177815.1	serine/threonine-protein kinase BRSK1 isoform X2
NM_001003920.3	NP_001003920.2	serine/threonine-protein kinase BRSK1 isoform 1
NM_001168572.1	NP_001162044.1	serine/threonine-protein kinase BRSK1 isoform 2
XM_017322325.2	XP_017177814.1	serine/threonine-protein kinase BRSK1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BRSK1	XP_541413.3
Rattus norvegicus (Norway rat)	Brsk1	NP_001120809.1
Danio rerio (zebrafish)	LOC558390	XP_003198179.2
Bos taurus (cattle)	BRSK1	XP_002695456.3
Xenopus tropicalis (tropical clawed frog)	brsk1	XP_004916365.1
Homo sapiens (human)	BRSK1	NP_115806.1
Mus musculus (house mouse)	Brsk1	NP_001003920.2
Danio rerio (zebrafish)	si:ch211-255p10.4	XP_693123.4

Gene Ontology
Bibliography

Related articles in PubMed

Structure and inhibition analysis of the mouse SAD-B C-terminal fragment.
Ma H, Wu JX, Wang J, Wang ZX, Wu JW
Bioscience, biotechnology, and biochemistry80(10)1939-46(2016 Oct)

SAD-B Phosphorylation of CAST Controls Active Zone Vesicle Recycling for Synaptic Depression.
Mochida S, Hida Y, Tanifuji S, Hagiwara A, Hamada S, Abe M, Ma H, Yasumura M, Kitajima I, Sakimura K, Ohtsuka T
Cell reports16(11)2901-2913(2016 09)

SAD-B kinase regulates pre-synaptic vesicular dynamics at hippocampal Schaffer collateral synapses and affects contextual fear memory.
Watabe AM, Nagase M, Hagiwara A, Hida Y, Tsuji M, Ochiai T, Kato F, Ohtsuka T
Journal of neurochemistry136(1)36-47(2016 Jan)

LKB1 and AMPK regulate synaptic remodeling in old age.
Samuel MA, Voinescu PE, Lilley BN, de Cabo R, Foretz M, Viollet B, Pawlyk B, Sandberg MA, Vavvas DG, Sanes JR
Nature neuroscience17(9)1190-7(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Structure and inhibition analysis of the mouse SAD-B C-terminal fragment
Title: Structure and inhibition analysis of the mouse SAD-B C-terminal fragment.

CAST/SAD-B reaction serves as a brake on synaptic transmission
Title: SAD-B Phosphorylation of CAST Controls Active Zone Vesicle Recycling for Synaptic Depression.

At mature pre-synaptic terminals, SAD-B regulates vesicular release probability and synaptic plasticity.
Title: SAD-B kinase regulates pre-synaptic vesicular dynamics at hippocampal Schaffer collateral synapses and affects contextual fear memory.

SAD-A and SAD-B kinases have multiple, sequential roles in axonal differentiation
Title: SAD kinases control the maturation of nerve terminals in the mammalian peripheral and central nervous systems.

SAD kinase may regulate neurotransmitter release from motor end plates in a similar manner to its regulation of neurotransmitter release in the central nervous system.
Title: Distribution of serine/threonine kinase SAD-B in mouse peripheral nerve synapse.

The following Brsk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brsk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15967	NM_001168572.1 Latest version!	Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu16224	NM_001003920.3 Latest version!	Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$454.30 $649.00
OMu82127	XM_017322325.2 Latest version!	Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu82128	XM_017322326.2 Latest version!	Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu82128	XM_017322326.1	Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu82127	XM_017322325.1	Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brsk1 ( NM_001168572.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001162044.1 Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15967
Clone ID Related Accession (Same CDS sequence)	NM_001168572.1
Accession Version	NM_001168572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase BRSK1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC086636.1, AC161197.9 and EU016556.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU016556.1, EU016557.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTCCG GGTCCAAGGA AGGTGGCGGG GGCTCCCCCG CCTACCACCT CCCACACCCA 
CACCCACACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGCCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGTCT AGTTAAACTT GGAGTCCACT GCATCACGGG TCAGAAGGTC 
GCTGTCAAGA TCGTGAACAG GGAGAAGCTG TCGGAATCGG TGCTGATGAA GGTGGAGAGG 
GAAATCGCCA TCCTGAAGCT CATTGAACAC CCGCACGTGC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTATA CTTGGTTCTT GAGCACGTTT CTGGTGGTGA GCTGTTCGAC 
TACCTGGTAA AAAAAGGGAG ACTGACACCC AAGGAGGCCC GGAAGTTCTT CCGCCAGATC 
GTGTCAGCAC TGGACTTCTG CCATAGCTAC TCCATCTGTC ACAGAGACTT GAAGCCAGAG 
AACCTGCTGT TGGATGAGAA AAACAACATC CGCATCGCAG ACTTTGGTAT GGCGTCCCTG 
CAAGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTACGC ATGTCCAGAG 
GTGATCAAGG GGGAAAAGTA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTATTTGCCC TGCTTGTGGG GGCACTGCCC TTCGATGACG ACAACCTGCG CCAGCTACTG 
GAGAAGGTGA AACGTGGGGT CTTCCACATG CCTCACTTCA TCCCTCCAGA CTGCCAGAGC 
CTCCTGAGAG GGATGATTGA AGTGGAGCCC GAGAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTATCTGGG CGGGAAACAC GAACCAGACC CTTGCCTGGA GCCAGCCCCA 
GGCCGCAGAG TAGCCATGCG TAGCCTGCCT TCCAATGGCG AGCTGGACCC TGACGTTCTG 
GAAAGCATGG CGTCTCTGGG CTGCTTCAGA GACCGCGAGC GGCTGCACAG AGAACTGCGA 
AGCGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162044.1
CDS	153..1184
Translation	MSSGSKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAVKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEE

Target ORF information:

RefSeq Version	NM_001168572.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168572.1

ORF Insert Sequence:

ATGTCGTCCG GGTCCAAGGA AGGTGGCGGG GGCTCCCCCG CCTACCACCT CCCACACCCA 
CACCCACACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGCCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGTCT AGTTAAACTT GGAGTCCACT GCATCACGGG TCAGAAGGTC 
GCTGTCAAGA TCGTGAACAG GGAGAAGCTG TCGGAATCGG TGCTGATGAA GGTGGAGAGG 
GAAATCGCCA TCCTGAAGCT CATTGAACAC CCGCACGTGC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTATA CTTGGTTCTT GAGCACGTTT CTGGTGGTGA GCTGTTCGAC 
TACCTGGTAA AAAAAGGGAG ACTGACACCC AAGGAGGCCC GGAAGTTCTT CCGCCAGATC 
GTGTCAGCAC TGGACTTCTG CCATAGCTAC TCCATCTGTC ACAGAGACTT GAAGCCAGAG 
AACCTGCTGT TGGATGAGAA AAACAACATC CGCATCGCAG ACTTTGGTAT GGCGTCCCTG 
CAAGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTACGC ATGTCCAGAG 
GTGATCAAGG GGGAAAAGTA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTATTTGCCC TGCTTGTGGG GGCACTGCCC TTCGATGACG ACAACCTGCG CCAGCTACTG 
GAGAAGGTGA AACGTGGGGT CTTCCACATG CCTCACTTCA TCCCTCCAGA CTGCCAGAGC 
CTCCTGAGAG GGATGATTGA AGTGGAGCCC GAGAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTATCTGGG CGGGAAACAC GAACCAGACC CTTGCCTGGA GCCAGCCCCA 
GGCCGCAGAG TAGCCATGCG TAGCCTGCCT TCCAATGGCG AGCTGGACCC TGACGTTCTG 
GAAAGCATGG CGTCTCTGGG CTGCTTCAGA GACCGCGAGC GGCTGCACAG AGAACTGCGA 
AGCGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brsk1 ( NM_001003920.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001003920.2 Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16224
Clone ID Related Accession (Same CDS sequence)	NM_001003920.3
Accession Version	NM_001003920.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase BRSK1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC086636.1 and AC161197.9. On Dec 10, 2009 this sequence version replaced NM_001003920.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC086636.1, AY533671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTCCG GGTCCAAGGA AGGTGGCGGG GGCTCCCCCG CCTACCACCT CCCACACCCA 
CACCCACACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGCCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGTCT AGTTAAACTT GGAGTCCACT GCATCACGGG TCAGAAGGTC 
GCTGTCAAGA TCGTGAACAG GGAGAAGCTG TCGGAATCGG TGCTGATGAA GGTGGAGAGG 
GAAATCGCCA TCCTGAAGCT CATTGAACAC CCGCACGTGC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTATA CTTGGTTCTT GAGCACGTTT CTGGTGGTGA GCTGTTCGAC 
TACCTGGTAA AAAAAGGGAG ACTGACACCC AAGGAGGCCC GGAAGTTCTT CCGCCAGATC 
GTGTCAGCAC TGGACTTCTG CCATAGCTAC TCCATCTGTC ACAGAGACTT GAAGCCAGAG 
AACCTGCTGT TGGATGAGAA AAACAACATC CGCATCGCAG ACTTTGGTAT GGCGTCCCTG 
CAAGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTACGC ATGTCCAGAG 
GTGATCAAGG GGGAAAAGTA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTATTTGCCC TGCTTGTGGG GGCACTGCCC TTCGATGACG ACAACCTGCG CCAGCTACTG 
GAGAAGGTGA AACGTGGGGT CTTCCACATG CCTCACTTCA TCCCTCCAGA CTGCCAGAGC 
CTCCTGAGAG GGATGATTGA AGTGGAGCCC GAGAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTATCTGGG CGGGAAACAC GAACCAGACC CTTGCCTGGA GCCAGCCCCA 
GGCCGCAGAG TAGCCATGCG TAGCCTGCCT TCCAATGGCG AGCTGGACCC TGACGTTCTG 
GAAAGCATGG CGTCTCTGGG CTGCTTCAGA GACCGCGAGC GGCTGCACAG AGAACTGCGA 
AGCGAGGAGG AAAACCAAGA AAAGATGATC TATTATTTGC TTTTGGATCG GAAAGAGCGG 
TATCCTAGCT GTGAAGACCA GGACCTGCCT CCTCGGAATG ATGTTGACCC ACCTCGTAAG 
CGTGTGGATT CCCCCATGCT GAGCAGACAC GGGAAACGGA GGCCAGAGCG GAAGTCCATG 
GAAGTCCTCA GTATCACAGA TGCTGGGAGT GGTGGCTCCC CAGTGCCTAC CAGACGGGCC 
TTGGAGATGG CCCAGCACAG TCAGAGATCC CGCAGCGTCA GTGGAGCCTC CACTGGTCTG 
TCCTCCAGCC CTCTGAGCAG CCCAAGGAGT CCGGTCTTTT CCTTCTCACC GGAGCCAGGC 
GCTGGAGATG AGGCTAGAGG CGGAGGCTCC CCAACTTCCA AAACACAGAC GCTGCCTTCT 
CGGGGCCCCA GAGGTGGGGG CGCTGGAGAG CAGCCACCAC CCCCCAGTGC CCGTTCCACG 
CCCTTGCCTG GACCACCAGG CTCCCCGCGC TCCTCCGGCG GAACCCCCTT GCACTCACCT 
CTGCACACGC CTCGAGCCAG CCCTACGGGG ACCCCAGGAA CGACGCCACC TCCCAGCCCA 
GGCGGTGGCG TCGGGGGAGC CGCCTGGAGA AGTCGGCTCA ACTCCATCCG CAACAGCTTC 
CTGGGCTCCC CTCGCTTCCA CCGGCGCAAG ATGCAGGTCC CTACCGCCGA GGAGATGTCC 
AGTTTGACAC CAGAATCCTC TCCTGAGCTG GCAAAACGCT CCTGGTTCGG GAATTTCATC 
TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 
AAAGCGGACA TTGTCCATGC CTTTCTGTCG ATCCCCAGCC TGAGTCACAG TGTACTGTCA 
CAGACCAGCT TCAGGGCCGA ATACAAGGCC AGCGGCGGCC CCTCCGTCTT CCAGAAGCCT 
GTCCGCTTTC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AACCCTCACC CCGAAGAGAT 
GGCAGCAGTG GAGGTGGCAT CTACTCAGTC ACCTTTACTC TCATCTCCGG TCCCAGCCGT 
CGGTTCAAAC GTGTGGTAGA GACCATCCAG GCACAGTTGC TGAGTACTCA CGACCAGCCC 
TCCGTGCAGG CCTTGGCAGA TGAGAAGAAC GGAGCCCAGA CCCGGCCTGC TGGGACCCCA 
CCCCGAAGCC TGCAGCCCCC ACCAGGCCGC TCTGACCCTG ATCTGAGTAG CTCTCCCCGC 
CGAGGACCCC CTAAGGACAA GAAGCTCCTG GCCACCAATG GAACCCCTCT ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003920.2
CDS	153..2489
Translation	MSSGSKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAVKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGSGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSSGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGTPPRSLQPPPGRSDPDLSSSPRRGPPKDKKLLATNGTPLP

Target ORF information:

RefSeq Version	NM_001003920.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003920.3

ORF Insert Sequence:

ATGTCGTCCG GGTCCAAGGA AGGTGGCGGG GGCTCCCCCG CCTACCACCT CCCACACCCA 
CACCCACACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGCCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGTCT AGTTAAACTT GGAGTCCACT GCATCACGGG TCAGAAGGTC 
GCTGTCAAGA TCGTGAACAG GGAGAAGCTG TCGGAATCGG TGCTGATGAA GGTGGAGAGG 
GAAATCGCCA TCCTGAAGCT CATTGAACAC CCGCACGTGC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTATA CTTGGTTCTT GAGCACGTTT CTGGTGGTGA GCTGTTCGAC 
TACCTGGTAA AAAAAGGGAG ACTGACACCC AAGGAGGCCC GGAAGTTCTT CCGCCAGATC 
GTGTCAGCAC TGGACTTCTG CCATAGCTAC TCCATCTGTC ACAGAGACTT GAAGCCAGAG 
AACCTGCTGT TGGATGAGAA AAACAACATC CGCATCGCAG ACTTTGGTAT GGCGTCCCTG 
CAAGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTACGC ATGTCCAGAG 
GTGATCAAGG GGGAAAAGTA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTATTTGCCC TGCTTGTGGG GGCACTGCCC TTCGATGACG ACAACCTGCG CCAGCTACTG 
GAGAAGGTGA AACGTGGGGT CTTCCACATG CCTCACTTCA TCCCTCCAGA CTGCCAGAGC 
CTCCTGAGAG GGATGATTGA AGTGGAGCCC GAGAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTATCTGGG CGGGAAACAC GAACCAGACC CTTGCCTGGA GCCAGCCCCA 
GGCCGCAGAG TAGCCATGCG TAGCCTGCCT TCCAATGGCG AGCTGGACCC TGACGTTCTG 
GAAAGCATGG CGTCTCTGGG CTGCTTCAGA GACCGCGAGC GGCTGCACAG AGAACTGCGA 
AGCGAGGAGG AAAACCAAGA AAAGATGATC TATTATTTGC TTTTGGATCG GAAAGAGCGG 
TATCCTAGCT GTGAAGACCA GGACCTGCCT CCTCGGAATG ATGTTGACCC ACCTCGTAAG 
CGTGTGGATT CCCCCATGCT GAGCAGACAC GGGAAACGGA GGCCAGAGCG GAAGTCCATG 
GAAGTCCTCA GTATCACAGA TGCTGGGAGT GGTGGCTCCC CAGTGCCTAC CAGACGGGCC 
TTGGAGATGG CCCAGCACAG TCAGAGATCC CGCAGCGTCA GTGGAGCCTC CACTGGTCTG 
TCCTCCAGCC CTCTGAGCAG CCCAAGGAGT CCGGTCTTTT CCTTCTCACC GGAGCCAGGC 
GCTGGAGATG AGGCTAGAGG CGGAGGCTCC CCAACTTCCA AAACACAGAC GCTGCCTTCT 
CGGGGCCCCA GAGGTGGGGG CGCTGGAGAG CAGCCACCAC CCCCCAGTGC CCGTTCCACG 
CCCTTGCCTG GACCACCAGG CTCCCCGCGC TCCTCCGGCG GAACCCCCTT GCACTCACCT 
CTGCACACGC CTCGAGCCAG CCCTACGGGG ACCCCAGGAA CGACGCCACC TCCCAGCCCA 
GGCGGTGGCG TCGGGGGAGC CGCCTGGAGA AGTCGGCTCA ACTCCATCCG CAACAGCTTC 
CTGGGCTCCC CTCGCTTCCA CCGGCGCAAG ATGCAGGTCC CTACCGCCGA GGAGATGTCC 
AGTTTGACAC CAGAATCCTC TCCTGAGCTG GCAAAACGCT CCTGGTTCGG GAATTTCATC 
TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 
AAAGCGGACA TTGTCCATGC CTTTCTGTCG ATCCCCAGCC TGAGTCACAG TGTACTGTCA 
CAGACCAGCT TCAGGGCCGA ATACAAGGCC AGCGGCGGCC CCTCCGTCTT CCAGAAGCCT 
GTCCGCTTTC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AACCCTCACC CCGAAGAGAT 
GGCAGCAGTG GAGGTGGCAT CTACTCAGTC ACCTTTACTC TCATCTCCGG TCCCAGCCGT 
CGGTTCAAAC GTGTGGTAGA GACCATCCAG GCACAGTTGC TGAGTACTCA CGACCAGCCC 
TCCGTGCAGG CCTTGGCAGA TGAGAAGAAC GGAGCCCAGA CCCGGCCTGC TGGGACCCCA 
CCCCGAAGCC TGCAGCCCCC ACCAGGCCGC TCTGACCCTG ATCTGAGTAG CTCTCCCCGC 
CGAGGACCCC CTAAGGACAA GAAGCTCCTG GCCACCAATG GAACCCCTCT ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brsk1 ( XM_017322325.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177814.1 Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu82127
Clone ID Related Accession (Same CDS sequence)	XM_017322325.1 , XM_017322325.2
Accession Version	XM_017322325.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase BRSK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCG GGTCCAAGGA AGGTGGCGGG GGCTCCCCCG CCTACCACCT CCCACACCCA 
CACCCACACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGCCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGTCT AGTTAAACTT GGAGTCCACT GCATCACGGG TCAGAAGGTC 
GCTGTCAAGA TCGTGAACAG GGAGAAGCTG TCGGAATCGG TGCTGATGAA GGTGGAGAGG 
GAAATCGCCA TCCTGAAGCT CATTGAACAC CCGCACGTGC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTATA CTTGGTTCTT GAGCACGTTT CTGGTGGTGA GCTGTTCGAC 
TACCTGGTAA AAAAAGGGAG ACTGACACCC AAGGAGGCCC GGAAGTTCTT CCGCCAGATC 
GTGTCAGCAC TGGACTTCTG CCATAGCTAC TCCATCTGTC ACAGAGACTT GAAGCCAGAG 
AACCTGCTGT TGGATGAGAA AAACAACATC CGCATCGCAG ACTTTGGTAT GGCGTCCCTG 
CAAGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTACGC ATGTCCAGAG 
GTGATCAAGG GGGAAAAGTA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTATTTGCCC TGCTTGTGGG GGCACTGCCC TTCGATGACG ACAACCTGCG CCAGCTACTG 
GAGAAGGTGA AACGTGGGGT CTTCCACATG CCTCACTTCA TCCCTCCAGA CTGCCAGAGC 
CTCCTGAGAG GGATGATTGA AGTGGAGCCC GAGAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTATCTGGG CGGGAAACAC GAACCAGACC CTTGCCTGGA GCCAGCCCCA 
GGCCGCAGAG TAGCCATGCG TAGCCTGCCT TCCAATGGCG AGCTGGACCC TGACGTTCTG 
GAAAGCATGG CGTCTCTGGG CTGCTTCAGA GACCGCGAGC GGCTGCACAG AGAACTGCGA 
AGCGAGGAGG AAAACCAAGA AAAGATGATC TATTATTTGC TTTTGGATCG GAAAGAGCGG 
TATCCTAGCT GTGAAGACCA GGACCTGCCT CCTCGGAATG ATGTTGACCC ACCTCGTAAG 
CGTGTGGATT CCCCCATGCT GAGCAGACAC GGGAAACGGA GGCCAGAGCG GAAGTCCATG 
GAAGTCCTCA GTATCACAGA TGCTGGGAGT GGTGGCTCCC CAGTGCCTAC CAGACGGGCC 
TTGGAGATGG CCCAGCACAG TCAGAGATCC CGCAGCGTCA GTGGAGCCTC CACTGGTCTG 
TCCTCCAGCC CTCTGAGCAG CCCAAGGAGT CCGGTCTTTT CCTTCTCACC GGAGCCAGGC 
GCTGGAGATG AGGCTAGAGG CGGAGGCTCC CCAACTTCCA AAACACAGAC GCTGCCTTCT 
CGGGGCCCCA GAGGTGGGGG CGCTGGAGAG CAGCCACCAC CCCCCAGTGC CCGTTCCACG 
CCCTTGCCTG GACCACCAGG CTCCCCGCGC TCCTCCGGCG GAACCCCCTT GCACTCACCT 
CTGCACACGC CTCGAGCCAG CCCTACGGGG ACCCCAGGAA CGACGCCACC TCCCAGCCCA 
GGCGGTGGCG TCGGGGGAGC CGCCTGGAGA AGTCGGCTCA ACTCCATCCG CAACAGCTTC 
CTGGGCTCCC CTCGCTTCCA CCGGCGCAAG ATGCAGGTCC CTACCGCCGA GGAGATGTCC 
AGTTTGACAC CAGAATCCTC TCCTGAGCTG GCAAAACGCT CCTGGTTCGG GAATTTCATC 
TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 
AAAGCGGACA TTGTCCATGC CTTTCTGTCG ATCCCCAGCC TGAGTCACAG TGTACTGTCA 
CAGACCAGCT TCAGGGCCGA ATACAAGGCC AGCGGCGGCC CCTCCGTCTT CCAGAAGCCT 
GTCCGCTTTC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AACCCTCACC CCGAAGAGAT 
GGCAGCAGTG GAGGTGGCAT CTACTCAGTC ACCTTTACTC TCATCTCCGG TCCCAGCCGT 
CGGTTCAAAC GTGTGGTAGA GACCATCCAG GCACAGTTGC TGAGTACTCA CGACCAGCCC 
TCCGTGCAGG CCTTGGCAGA TGAGAAGAAC GGAGCCCAGA CCCGGCCTGC TGGGACCCCA 
CCCCGAAGCC TGCAGCCCCC ACCAGGCCGC TCTGACCCTG ATCTGAGTAG CTCTCCCCGC 
CGAGGACCCC CTAAGGACAA GAAGCTCCTG GCCACCAATG GAACCCCTCT ACCCTGCCCC 
CCTACTGTGA ATCTGTATAT AAGGCCCAAG GAGTATGTTG GGAGGGGGGA CACAAAACTA 
GGCCTTGCCC TGCAGGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177814.1
CDS	332..2752
Translation	MSSGSKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAVKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGSGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSSGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGTPPRSLQPPPGRSDPDLSSSPRRGPPKDKKLLATNGTPLPCPPTVNLYIRPKEYVGRGDTKLGLALQG

Target ORF information:

RefSeq Version	XM_017322325.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322325.1

ORF Insert Sequence:

ATGTCGTCCG GGTCCAAGGA AGGTGGCGGG GGCTCCCCCG CCTACCACCT CCCACACCCA 
CACCCACACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGCCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGTCT AGTTAAACTT GGAGTCCACT GCATCACGGG TCAGAAGGTC 
GCTGTCAAGA TCGTGAACAG GGAGAAGCTG TCGGAATCGG TGCTGATGAA GGTGGAGAGG 
GAAATCGCCA TCCTGAAGCT CATTGAACAC CCGCACGTGC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTATA CTTGGTTCTT GAGCACGTTT CTGGTGGTGA GCTGTTCGAC 
TACCTGGTAA AAAAAGGGAG ACTGACACCC AAGGAGGCCC GGAAGTTCTT CCGCCAGATC 
GTGTCAGCAC TGGACTTCTG CCATAGCTAC TCCATCTGTC ACAGAGACTT GAAGCCAGAG 
AACCTGCTGT TGGATGAGAA AAACAACATC CGCATCGCAG ACTTTGGTAT GGCGTCCCTG 
CAAGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTACGC ATGTCCAGAG 
GTGATCAAGG GGGAAAAGTA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTATTTGCCC TGCTTGTGGG GGCACTGCCC TTCGATGACG ACAACCTGCG CCAGCTACTG 
GAGAAGGTGA AACGTGGGGT CTTCCACATG CCTCACTTCA TCCCTCCAGA CTGCCAGAGC 
CTCCTGAGAG GGATGATTGA AGTGGAGCCC GAGAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTATCTGGG CGGGAAACAC GAACCAGACC CTTGCCTGGA GCCAGCCCCA 
GGCCGCAGAG TAGCCATGCG TAGCCTGCCT TCCAATGGCG AGCTGGACCC TGACGTTCTG 
GAAAGCATGG CGTCTCTGGG CTGCTTCAGA GACCGCGAGC GGCTGCACAG AGAACTGCGA 
AGCGAGGAGG AAAACCAAGA AAAGATGATC TATTATTTGC TTTTGGATCG GAAAGAGCGG 
TATCCTAGCT GTGAAGACCA GGACCTGCCT CCTCGGAATG ATGTTGACCC ACCTCGTAAG 
CGTGTGGATT CCCCCATGCT GAGCAGACAC GGGAAACGGA GGCCAGAGCG GAAGTCCATG 
GAAGTCCTCA GTATCACAGA TGCTGGGAGT GGTGGCTCCC CAGTGCCTAC CAGACGGGCC 
TTGGAGATGG CCCAGCACAG TCAGAGATCC CGCAGCGTCA GTGGAGCCTC CACTGGTCTG 
TCCTCCAGCC CTCTGAGCAG CCCAAGGAGT CCGGTCTTTT CCTTCTCACC GGAGCCAGGC 
GCTGGAGATG AGGCTAGAGG CGGAGGCTCC CCAACTTCCA AAACACAGAC GCTGCCTTCT 
CGGGGCCCCA GAGGTGGGGG CGCTGGAGAG CAGCCACCAC CCCCCAGTGC CCGTTCCACG 
CCCTTGCCTG GACCACCAGG CTCCCCGCGC TCCTCCGGCG GAACCCCCTT GCACTCACCT 
CTGCACACGC CTCGAGCCAG CCCTACGGGG ACCCCAGGAA CGACGCCACC TCCCAGCCCA 
GGCGGTGGCG TCGGGGGAGC CGCCTGGAGA AGTCGGCTCA ACTCCATCCG CAACAGCTTC 
CTGGGCTCCC CTCGCTTCCA CCGGCGCAAG ATGCAGGTCC CTACCGCCGA GGAGATGTCC 
AGTTTGACAC CAGAATCCTC TCCTGAGCTG GCAAAACGCT CCTGGTTCGG GAATTTCATC 
TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 
AAAGCGGACA TTGTCCATGC CTTTCTGTCG ATCCCCAGCC TGAGTCACAG TGTACTGTCA 
CAGACCAGCT TCAGGGCCGA ATACAAGGCC AGCGGCGGCC CCTCCGTCTT CCAGAAGCCT 
GTCCGCTTTC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AACCCTCACC CCGAAGAGAT 
GGCAGCAGTG GAGGTGGCAT CTACTCAGTC ACCTTTACTC TCATCTCCGG TCCCAGCCGT 
CGGTTCAAAC GTGTGGTAGA GACCATCCAG GCACAGTTGC TGAGTACTCA CGACCAGCCC 
TCCGTGCAGG CCTTGGCAGA TGAGAAGAAC GGAGCCCAGA CCCGGCCTGC TGGGACCCCA 
CCCCGAAGCC TGCAGCCCCC ACCAGGCCGC TCTGACCCTG ATCTGAGTAG CTCTCCCCGC 
CGAGGACCCC CTAAGGACAA GAAGCTCCTG GCCACCAATG GAACCCCTCT ACCCTGCCCC 
CCTACTGTGA ATCTGTATAT AAGGCCCAAG GAGTATGTTG GGAGGGGGGA CACAAAACTA 
GGCCTTGCCC TGCAGGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brsk1 ( XM_017322325.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177814.1 Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu82127
Clone ID Related Accession (Same CDS sequence)	XM_017322325.1 , XM_017322325.2
Accession Version	XM_017322325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase BRSK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017322325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCG GGTCCAAGGA AGGTGGCGGG GGCTCCCCCG CCTACCACCT CCCACACCCA 
CACCCACACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGCCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGTCT AGTTAAACTT GGAGTCCACT GCATCACGGG TCAGAAGGTC 
GCTGTCAAGA TCGTGAACAG GGAGAAGCTG TCGGAATCGG TGCTGATGAA GGTGGAGAGG 
GAAATCGCCA TCCTGAAGCT CATTGAACAC CCGCACGTGC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTATA CTTGGTTCTT GAGCACGTTT CTGGTGGTGA GCTGTTCGAC 
TACCTGGTAA AAAAAGGGAG ACTGACACCC AAGGAGGCCC GGAAGTTCTT CCGCCAGATC 
GTGTCAGCAC TGGACTTCTG CCATAGCTAC TCCATCTGTC ACAGAGACTT GAAGCCAGAG 
AACCTGCTGT TGGATGAGAA AAACAACATC CGCATCGCAG ACTTTGGTAT GGCGTCCCTG 
CAAGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTACGC ATGTCCAGAG 
GTGATCAAGG GGGAAAAGTA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTATTTGCCC TGCTTGTGGG GGCACTGCCC TTCGATGACG ACAACCTGCG CCAGCTACTG 
GAGAAGGTGA AACGTGGGGT CTTCCACATG CCTCACTTCA TCCCTCCAGA CTGCCAGAGC 
CTCCTGAGAG GGATGATTGA AGTGGAGCCC GAGAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTATCTGGG CGGGAAACAC GAACCAGACC CTTGCCTGGA GCCAGCCCCA 
GGCCGCAGAG TAGCCATGCG TAGCCTGCCT TCCAATGGCG AGCTGGACCC TGACGTTCTG 
GAAAGCATGG CGTCTCTGGG CTGCTTCAGA GACCGCGAGC GGCTGCACAG AGAACTGCGA 
AGCGAGGAGG AAAACCAAGA AAAGATGATC TATTATTTGC TTTTGGATCG GAAAGAGCGG 
TATCCTAGCT GTGAAGACCA GGACCTGCCT CCTCGGAATG ATGTTGACCC ACCTCGTAAG 
CGTGTGGATT CCCCCATGCT GAGCAGACAC GGGAAACGGA GGCCAGAGCG GAAGTCCATG 
GAAGTCCTCA GTATCACAGA TGCTGGGAGT GGTGGCTCCC CAGTGCCTAC CAGACGGGCC 
TTGGAGATGG CCCAGCACAG TCAGAGATCC CGCAGCGTCA GTGGAGCCTC CACTGGTCTG 
TCCTCCAGCC CTCTGAGCAG CCCAAGGAGT CCGGTCTTTT CCTTCTCACC GGAGCCAGGC 
GCTGGAGATG AGGCTAGAGG CGGAGGCTCC CCAACTTCCA AAACACAGAC GCTGCCTTCT 
CGGGGCCCCA GAGGTGGGGG CGCTGGAGAG CAGCCACCAC CCCCCAGTGC CCGTTCCACG 
CCCTTGCCTG GACCACCAGG CTCCCCGCGC TCCTCCGGCG GAACCCCCTT GCACTCACCT 
CTGCACACGC CTCGAGCCAG CCCTACGGGG ACCCCAGGAA CGACGCCACC TCCCAGCCCA 
GGCGGTGGCG TCGGGGGAGC CGCCTGGAGA AGTCGGCTCA ACTCCATCCG CAACAGCTTC 
CTGGGCTCCC CTCGCTTCCA CCGGCGCAAG ATGCAGGTCC CTACCGCCGA GGAGATGTCC 
AGTTTGACAC CAGAATCCTC TCCTGAGCTG GCAAAACGCT CCTGGTTCGG GAATTTCATC 
TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 
AAAGCGGACA TTGTCCATGC CTTTCTGTCG ATCCCCAGCC TGAGTCACAG TGTACTGTCA 
CAGACCAGCT TCAGGGCCGA ATACAAGGCC AGCGGCGGCC CCTCCGTCTT CCAGAAGCCT 
GTCCGCTTTC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AACCCTCACC CCGAAGAGAT 
GGCAGCAGTG GAGGTGGCAT CTACTCAGTC ACCTTTACTC TCATCTCCGG TCCCAGCCGT 
CGGTTCAAAC GTGTGGTAGA GACCATCCAG GCACAGTTGC TGAGTACTCA CGACCAGCCC 
TCCGTGCAGG CCTTGGCAGA TGAGAAGAAC GGAGCCCAGA CCCGGCCTGC TGGGACCCCA 
CCCCGAAGCC TGCAGCCCCC ACCAGGCCGC TCTGACCCTG ATCTGAGTAG CTCTCCCCGC 
CGAGGACCCC CTAAGGACAA GAAGCTCCTG GCCACCAATG GAACCCCTCT ACCCTGCCCC 
CCTACTGTGA ATCTGTATAT AAGGCCCAAG GAGTATGTTG GGAGGGGGGA CACAAAACTA 
GGCCTTGCCC TGCAGGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177814.1
CDS	445..2865
Translation	MSSGSKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAVKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGSGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSSGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGTPPRSLQPPPGRSDPDLSSSPRRGPPKDKKLLATNGTPLPCPPTVNLYIRPKEYVGRGDTKLGLALQG

Target ORF information:

RefSeq Version	XM_017322325.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322325.2

ORF Insert Sequence:

ATGTCGTCCG GGTCCAAGGA AGGTGGCGGG GGCTCCCCCG CCTACCACCT CCCACACCCA 
CACCCACACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGCCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGTCT AGTTAAACTT GGAGTCCACT GCATCACGGG TCAGAAGGTC 
GCTGTCAAGA TCGTGAACAG GGAGAAGCTG TCGGAATCGG TGCTGATGAA GGTGGAGAGG 
GAAATCGCCA TCCTGAAGCT CATTGAACAC CCGCACGTGC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTATA CTTGGTTCTT GAGCACGTTT CTGGTGGTGA GCTGTTCGAC 
TACCTGGTAA AAAAAGGGAG ACTGACACCC AAGGAGGCCC GGAAGTTCTT CCGCCAGATC 
GTGTCAGCAC TGGACTTCTG CCATAGCTAC TCCATCTGTC ACAGAGACTT GAAGCCAGAG 
AACCTGCTGT TGGATGAGAA AAACAACATC CGCATCGCAG ACTTTGGTAT GGCGTCCCTG 
CAAGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTACGC ATGTCCAGAG 
GTGATCAAGG GGGAAAAGTA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTATTTGCCC TGCTTGTGGG GGCACTGCCC TTCGATGACG ACAACCTGCG CCAGCTACTG 
GAGAAGGTGA AACGTGGGGT CTTCCACATG CCTCACTTCA TCCCTCCAGA CTGCCAGAGC 
CTCCTGAGAG GGATGATTGA AGTGGAGCCC GAGAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTATCTGGG CGGGAAACAC GAACCAGACC CTTGCCTGGA GCCAGCCCCA 
GGCCGCAGAG TAGCCATGCG TAGCCTGCCT TCCAATGGCG AGCTGGACCC TGACGTTCTG 
GAAAGCATGG CGTCTCTGGG CTGCTTCAGA GACCGCGAGC GGCTGCACAG AGAACTGCGA 
AGCGAGGAGG AAAACCAAGA AAAGATGATC TATTATTTGC TTTTGGATCG GAAAGAGCGG 
TATCCTAGCT GTGAAGACCA GGACCTGCCT CCTCGGAATG ATGTTGACCC ACCTCGTAAG 
CGTGTGGATT CCCCCATGCT GAGCAGACAC GGGAAACGGA GGCCAGAGCG GAAGTCCATG 
GAAGTCCTCA GTATCACAGA TGCTGGGAGT GGTGGCTCCC CAGTGCCTAC CAGACGGGCC 
TTGGAGATGG CCCAGCACAG TCAGAGATCC CGCAGCGTCA GTGGAGCCTC CACTGGTCTG 
TCCTCCAGCC CTCTGAGCAG CCCAAGGAGT CCGGTCTTTT CCTTCTCACC GGAGCCAGGC 
GCTGGAGATG AGGCTAGAGG CGGAGGCTCC CCAACTTCCA AAACACAGAC GCTGCCTTCT 
CGGGGCCCCA GAGGTGGGGG CGCTGGAGAG CAGCCACCAC CCCCCAGTGC CCGTTCCACG 
CCCTTGCCTG GACCACCAGG CTCCCCGCGC TCCTCCGGCG GAACCCCCTT GCACTCACCT 
CTGCACACGC CTCGAGCCAG CCCTACGGGG ACCCCAGGAA CGACGCCACC TCCCAGCCCA 
GGCGGTGGCG TCGGGGGAGC CGCCTGGAGA AGTCGGCTCA ACTCCATCCG CAACAGCTTC 
CTGGGCTCCC CTCGCTTCCA CCGGCGCAAG ATGCAGGTCC CTACCGCCGA GGAGATGTCC 
AGTTTGACAC CAGAATCCTC TCCTGAGCTG GCAAAACGCT CCTGGTTCGG GAATTTCATC 
TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 
AAAGCGGACA TTGTCCATGC CTTTCTGTCG ATCCCCAGCC TGAGTCACAG TGTACTGTCA 
CAGACCAGCT TCAGGGCCGA ATACAAGGCC AGCGGCGGCC CCTCCGTCTT CCAGAAGCCT 
GTCCGCTTTC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AACCCTCACC CCGAAGAGAT 
GGCAGCAGTG GAGGTGGCAT CTACTCAGTC ACCTTTACTC TCATCTCCGG TCCCAGCCGT 
CGGTTCAAAC GTGTGGTAGA GACCATCCAG GCACAGTTGC TGAGTACTCA CGACCAGCCC 
TCCGTGCAGG CCTTGGCAGA TGAGAAGAAC GGAGCCCAGA CCCGGCCTGC TGGGACCCCA 
CCCCGAAGCC TGCAGCCCCC ACCAGGCCGC TCTGACCCTG ATCTGAGTAG CTCTCCCCGC 
CGAGGACCCC CTAAGGACAA GAAGCTCCTG GCCACCAATG GAACCCCTCT ACCCTGCCCC 
CCTACTGTGA ATCTGTATAT AAGGCCCAAG GAGTATGTTG GGAGGGGGGA CACAAAACTA 
GGCCTTGCCC TGCAGGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brsk1 ( XM_017322326.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177815.1 Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu82128
Clone ID Related Accession (Same CDS sequence)	XM_017322326.1 , XM_017322326.2
Accession Version	XM_017322326.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase BRSK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCC TGCAAGTGGG GGACAGCCTC CTGGAGACCA GCTGCGGGTC CCCCCATTAC 
GCATGTCCAG AGGTGATCAA GGGGGAAAAG TATGATGGCC GCCGGGCAGA CATGTGGAGC 
TGTGGAGTCA TCCTATTTGC CCTGCTTGTG GGGGCACTGC CCTTCGATGA CGACAACCTG 
CGCCAGCTAC TGGAGAAGGT GAAACGTGGG GTCTTCCACA TGCCTCACTT CATCCCTCCA 
GACTGCCAGA GCCTCCTGAG AGGGATGATT GAAGTGGAGC CCGAGAAAAG GCTCAGTCTG 
GAGCAAATTC AGAAACATCC TTGGTATCTG GGCGGGAAAC ACGAACCAGA CCCTTGCCTG 
GAGCCAGCCC CAGGCCGCAG AGTAGCCATG CGTAGCCTGC CTTCCAATGG CGAGCTGGAC 
CCTGACGTTC TGGAAAGCAT GGCGTCTCTG GGCTGCTTCA GAGACCGCGA GCGGCTGCAC 
AGAGAACTGC GAAGCGAGGA GGAAAACCAA GAAAAGATGA TCTATTATTT GCTTTTGGAT 
CGGAAAGAGC GGTATCCTAG CTGTGAAGAC CAGGACCTGC CTCCTCGGAA TGATGTTGAC 
CCACCTCGTA AGCGTGTGGA TTCCCCCATG CTGAGCAGAC ACGGGAAACG GAGGCCAGAG 
CGGAAGTCCA TGGAAGTCCT CAGTATCACA GATGCTGGGA GTGGTGGCTC CCCAGTGCCT 
ACCAGACGGG CCTTGGAGAT GGCCCAGCAC AGTCAGAGAT CCCGCAGCGT CAGTGGAGCC 
TCCACTGGTC TGTCCTCCAG CCCTCTGAGC AGCCCAAGGA GTCCGGTCTT TTCCTTCTCA 
CCGGAGCCAG GCGCTGGAGA TGAGGCTAGA GGCGGAGGCT CCCCAACTTC CAAAACACAG 
ACGCTGCCTT CTCGGGGCCC CAGAGGTGGG GGCGCTGGAG AGCAGCCACC ACCCCCCAGT 
GCCCGTTCCA CGCCCTTGCC TGGACCACCA GGCTCCCCGC GCTCCTCCGG CGGAACCCCC 
TTGCACTCAC CTCTGCACAC GCCTCGAGCC AGCCCTACGG GGACCCCAGG AACGACGCCA 
CCTCCCAGCC CAGGCGGTGG CGTCGGGGGA GCCGCCTGGA GAAGTCGGCT CAACTCCATC 
CGCAACAGCT TCCTGGGCTC CCCTCGCTTC CACCGGCGCA AGATGCAGGT CCCTACCGCC 
GAGGAGATGT CCAGTTTGAC ACCAGAATCC TCTCCTGAGC TGGCAAAACG CTCCTGGTTC 
GGGAATTTCA TCTCCTTGGA CAAAGAAGAA CAAATATTCC TCGTGCTAAA GGACAAACCT 
CTCAGCAGCA TCAAAGCGGA CATTGTCCAT GCCTTTCTGT CGATCCCCAG CCTGAGTCAC 
AGTGTACTGT CACAGACCAG CTTCAGGGCC GAATACAAGG CCAGCGGCGG CCCCTCCGTC 
TTCCAGAAGC CTGTCCGCTT TCAGGTGGAC ATCAGCTCCT CTGAGGGTCC AGAACCCTCA 
CCCCGAAGAG ATGGCAGCAG TGGAGGTGGC ATCTACTCAG TCACCTTTAC TCTCATCTCC 
GGTCCCAGCC GTCGGTTCAA ACGTGTGGTA GAGACCATCC AGGCACAGTT GCTGAGTACT 
CACGACCAGC CCTCCGTGCA GGCCTTGGCA GATGAGAAGA ACGGAGCCCA GACCCGGCCT 
GCTGGGACCC CACCCCGAAG CCTGCAGCCC CCACCAGGCC GCTCTGACCC TGATCTGAGT 
AGCTCTCCCC GCCGAGGACC CCCTAAGGAC AAGAAGCTCC TGGCCACCAA TGGAACCCCT 
CTACCCTGCC CCCCTACTGT GAATCTGTAT ATAAGGCCCA AGGAGTATGT TGGGAGGGGG 
GACACAAAAC TAGGCCTTGC CCTGCAGGGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177815.1
CDS	397..2289
Translation	MASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGSGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSSGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGTPPRSLQPPPGRSDPDLSSSPRRGPPKDKKLLATNGTPLPCPPTVNLYIRPKEYVGRGDTKLGLALQG

Target ORF information:

RefSeq Version	XM_017322326.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322326.1

ORF Insert Sequence:

ATGGCGTCCC TGCAAGTGGG GGACAGCCTC CTGGAGACCA GCTGCGGGTC CCCCCATTAC 
GCATGTCCAG AGGTGATCAA GGGGGAAAAG TATGATGGCC GCCGGGCAGA CATGTGGAGC 
TGTGGAGTCA TCCTATTTGC CCTGCTTGTG GGGGCACTGC CCTTCGATGA CGACAACCTG 
CGCCAGCTAC TGGAGAAGGT GAAACGTGGG GTCTTCCACA TGCCTCACTT CATCCCTCCA 
GACTGCCAGA GCCTCCTGAG AGGGATGATT GAAGTGGAGC CCGAGAAAAG GCTCAGTCTG 
GAGCAAATTC AGAAACATCC TTGGTATCTG GGCGGGAAAC ACGAACCAGA CCCTTGCCTG 
GAGCCAGCCC CAGGCCGCAG AGTAGCCATG CGTAGCCTGC CTTCCAATGG CGAGCTGGAC 
CCTGACGTTC TGGAAAGCAT GGCGTCTCTG GGCTGCTTCA GAGACCGCGA GCGGCTGCAC 
AGAGAACTGC GAAGCGAGGA GGAAAACCAA GAAAAGATGA TCTATTATTT GCTTTTGGAT 
CGGAAAGAGC GGTATCCTAG CTGTGAAGAC CAGGACCTGC CTCCTCGGAA TGATGTTGAC 
CCACCTCGTA AGCGTGTGGA TTCCCCCATG CTGAGCAGAC ACGGGAAACG GAGGCCAGAG 
CGGAAGTCCA TGGAAGTCCT CAGTATCACA GATGCTGGGA GTGGTGGCTC CCCAGTGCCT 
ACCAGACGGG CCTTGGAGAT GGCCCAGCAC AGTCAGAGAT CCCGCAGCGT CAGTGGAGCC 
TCCACTGGTC TGTCCTCCAG CCCTCTGAGC AGCCCAAGGA GTCCGGTCTT TTCCTTCTCA 
CCGGAGCCAG GCGCTGGAGA TGAGGCTAGA GGCGGAGGCT CCCCAACTTC CAAAACACAG 
ACGCTGCCTT CTCGGGGCCC CAGAGGTGGG GGCGCTGGAG AGCAGCCACC ACCCCCCAGT 
GCCCGTTCCA CGCCCTTGCC TGGACCACCA GGCTCCCCGC GCTCCTCCGG CGGAACCCCC 
TTGCACTCAC CTCTGCACAC GCCTCGAGCC AGCCCTACGG GGACCCCAGG AACGACGCCA 
CCTCCCAGCC CAGGCGGTGG CGTCGGGGGA GCCGCCTGGA GAAGTCGGCT CAACTCCATC 
CGCAACAGCT TCCTGGGCTC CCCTCGCTTC CACCGGCGCA AGATGCAGGT CCCTACCGCC 
GAGGAGATGT CCAGTTTGAC ACCAGAATCC TCTCCTGAGC TGGCAAAACG CTCCTGGTTC 
GGGAATTTCA TCTCCTTGGA CAAAGAAGAA CAAATATTCC TCGTGCTAAA GGACAAACCT 
CTCAGCAGCA TCAAAGCGGA CATTGTCCAT GCCTTTCTGT CGATCCCCAG CCTGAGTCAC 
AGTGTACTGT CACAGACCAG CTTCAGGGCC GAATACAAGG CCAGCGGCGG CCCCTCCGTC 
TTCCAGAAGC CTGTCCGCTT TCAGGTGGAC ATCAGCTCCT CTGAGGGTCC AGAACCCTCA 
CCCCGAAGAG ATGGCAGCAG TGGAGGTGGC ATCTACTCAG TCACCTTTAC TCTCATCTCC 
GGTCCCAGCC GTCGGTTCAA ACGTGTGGTA GAGACCATCC AGGCACAGTT GCTGAGTACT 
CACGACCAGC CCTCCGTGCA GGCCTTGGCA GATGAGAAGA ACGGAGCCCA GACCCGGCCT 
GCTGGGACCC CACCCCGAAG CCTGCAGCCC CCACCAGGCC GCTCTGACCC TGATCTGAGT 
AGCTCTCCCC GCCGAGGACC CCCTAAGGAC AAGAAGCTCC TGGCCACCAA TGGAACCCCT 
CTACCCTGCC CCCCTACTGT GAATCTGTAT ATAAGGCCCA AGGAGTATGT TGGGAGGGGG 
GACACAAAAC TAGGCCTTGC CCTGCAGGGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brsk1 ( XM_017322326.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177815.1 Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant X2, mRNA.

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CloneID	OMu82128
Clone ID Related Accession (Same CDS sequence)	XM_017322326.1 , XM_017322326.2
Accession Version	XM_017322326.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase BRSK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017322326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCC TGCAAGTGGG GGACAGCCTC CTGGAGACCA GCTGCGGGTC CCCCCATTAC 
GCATGTCCAG AGGTGATCAA GGGGGAAAAG TATGATGGCC GCCGGGCAGA CATGTGGAGC 
TGTGGAGTCA TCCTATTTGC CCTGCTTGTG GGGGCACTGC CCTTCGATGA CGACAACCTG 
CGCCAGCTAC TGGAGAAGGT GAAACGTGGG GTCTTCCACA TGCCTCACTT CATCCCTCCA 
GACTGCCAGA GCCTCCTGAG AGGGATGATT GAAGTGGAGC CCGAGAAAAG GCTCAGTCTG 
GAGCAAATTC AGAAACATCC TTGGTATCTG GGCGGGAAAC ACGAACCAGA CCCTTGCCTG 
GAGCCAGCCC CAGGCCGCAG AGTAGCCATG CGTAGCCTGC CTTCCAATGG CGAGCTGGAC 
CCTGACGTTC TGGAAAGCAT GGCGTCTCTG GGCTGCTTCA GAGACCGCGA GCGGCTGCAC 
AGAGAACTGC GAAGCGAGGA GGAAAACCAA GAAAAGATGA TCTATTATTT GCTTTTGGAT 
CGGAAAGAGC GGTATCCTAG CTGTGAAGAC CAGGACCTGC CTCCTCGGAA TGATGTTGAC 
CCACCTCGTA AGCGTGTGGA TTCCCCCATG CTGAGCAGAC ACGGGAAACG GAGGCCAGAG 
CGGAAGTCCA TGGAAGTCCT CAGTATCACA GATGCTGGGA GTGGTGGCTC CCCAGTGCCT 
ACCAGACGGG CCTTGGAGAT GGCCCAGCAC AGTCAGAGAT CCCGCAGCGT CAGTGGAGCC 
TCCACTGGTC TGTCCTCCAG CCCTCTGAGC AGCCCAAGGA GTCCGGTCTT TTCCTTCTCA 
CCGGAGCCAG GCGCTGGAGA TGAGGCTAGA GGCGGAGGCT CCCCAACTTC CAAAACACAG 
ACGCTGCCTT CTCGGGGCCC CAGAGGTGGG GGCGCTGGAG AGCAGCCACC ACCCCCCAGT 
GCCCGTTCCA CGCCCTTGCC TGGACCACCA GGCTCCCCGC GCTCCTCCGG CGGAACCCCC 
TTGCACTCAC CTCTGCACAC GCCTCGAGCC AGCCCTACGG GGACCCCAGG AACGACGCCA 
CCTCCCAGCC CAGGCGGTGG CGTCGGGGGA GCCGCCTGGA GAAGTCGGCT CAACTCCATC 
CGCAACAGCT TCCTGGGCTC CCCTCGCTTC CACCGGCGCA AGATGCAGGT CCCTACCGCC 
GAGGAGATGT CCAGTTTGAC ACCAGAATCC TCTCCTGAGC TGGCAAAACG CTCCTGGTTC 
GGGAATTTCA TCTCCTTGGA CAAAGAAGAA CAAATATTCC TCGTGCTAAA GGACAAACCT 
CTCAGCAGCA TCAAAGCGGA CATTGTCCAT GCCTTTCTGT CGATCCCCAG CCTGAGTCAC 
AGTGTACTGT CACAGACCAG CTTCAGGGCC GAATACAAGG CCAGCGGCGG CCCCTCCGTC 
TTCCAGAAGC CTGTCCGCTT TCAGGTGGAC ATCAGCTCCT CTGAGGGTCC AGAACCCTCA 
CCCCGAAGAG ATGGCAGCAG TGGAGGTGGC ATCTACTCAG TCACCTTTAC TCTCATCTCC 
GGTCCCAGCC GTCGGTTCAA ACGTGTGGTA GAGACCATCC AGGCACAGTT GCTGAGTACT 
CACGACCAGC CCTCCGTGCA GGCCTTGGCA GATGAGAAGA ACGGAGCCCA GACCCGGCCT 
GCTGGGACCC CACCCCGAAG CCTGCAGCCC CCACCAGGCC GCTCTGACCC TGATCTGAGT 
AGCTCTCCCC GCCGAGGACC CCCTAAGGAC AAGAAGCTCC TGGCCACCAA TGGAACCCCT 
CTACCCTGCC CCCCTACTGT GAATCTGTAT ATAAGGCCCA AGGAGTATGT TGGGAGGGGG 
GACACAAAAC TAGGCCTTGC CCTGCAGGGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177815.1
CDS	397..2289
Translation	MASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGSGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSSGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGTPPRSLQPPPGRSDPDLSSSPRRGPPKDKKLLATNGTPLPCPPTVNLYIRPKEYVGRGDTKLGLALQG

Target ORF information:

RefSeq Version	XM_017322326.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus BR serine/threonine kinase 1 (Brsk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322326.2

ORF Insert Sequence:

ATGGCGTCCC TGCAAGTGGG GGACAGCCTC CTGGAGACCA GCTGCGGGTC CCCCCATTAC 
GCATGTCCAG AGGTGATCAA GGGGGAAAAG TATGATGGCC GCCGGGCAGA CATGTGGAGC 
TGTGGAGTCA TCCTATTTGC CCTGCTTGTG GGGGCACTGC CCTTCGATGA CGACAACCTG 
CGCCAGCTAC TGGAGAAGGT GAAACGTGGG GTCTTCCACA TGCCTCACTT CATCCCTCCA 
GACTGCCAGA GCCTCCTGAG AGGGATGATT GAAGTGGAGC CCGAGAAAAG GCTCAGTCTG 
GAGCAAATTC AGAAACATCC TTGGTATCTG GGCGGGAAAC ACGAACCAGA CCCTTGCCTG 
GAGCCAGCCC CAGGCCGCAG AGTAGCCATG CGTAGCCTGC CTTCCAATGG CGAGCTGGAC 
CCTGACGTTC TGGAAAGCAT GGCGTCTCTG GGCTGCTTCA GAGACCGCGA GCGGCTGCAC 
AGAGAACTGC GAAGCGAGGA GGAAAACCAA GAAAAGATGA TCTATTATTT GCTTTTGGAT 
CGGAAAGAGC GGTATCCTAG CTGTGAAGAC CAGGACCTGC CTCCTCGGAA TGATGTTGAC 
CCACCTCGTA AGCGTGTGGA TTCCCCCATG CTGAGCAGAC ACGGGAAACG GAGGCCAGAG 
CGGAAGTCCA TGGAAGTCCT CAGTATCACA GATGCTGGGA GTGGTGGCTC CCCAGTGCCT 
ACCAGACGGG CCTTGGAGAT GGCCCAGCAC AGTCAGAGAT CCCGCAGCGT CAGTGGAGCC 
TCCACTGGTC TGTCCTCCAG CCCTCTGAGC AGCCCAAGGA GTCCGGTCTT TTCCTTCTCA 
CCGGAGCCAG GCGCTGGAGA TGAGGCTAGA GGCGGAGGCT CCCCAACTTC CAAAACACAG 
ACGCTGCCTT CTCGGGGCCC CAGAGGTGGG GGCGCTGGAG AGCAGCCACC ACCCCCCAGT 
GCCCGTTCCA CGCCCTTGCC TGGACCACCA GGCTCCCCGC GCTCCTCCGG CGGAACCCCC 
TTGCACTCAC CTCTGCACAC GCCTCGAGCC AGCCCTACGG GGACCCCAGG AACGACGCCA 
CCTCCCAGCC CAGGCGGTGG CGTCGGGGGA GCCGCCTGGA GAAGTCGGCT CAACTCCATC 
CGCAACAGCT TCCTGGGCTC CCCTCGCTTC CACCGGCGCA AGATGCAGGT CCCTACCGCC 
GAGGAGATGT CCAGTTTGAC ACCAGAATCC TCTCCTGAGC TGGCAAAACG CTCCTGGTTC 
GGGAATTTCA TCTCCTTGGA CAAAGAAGAA CAAATATTCC TCGTGCTAAA GGACAAACCT 
CTCAGCAGCA TCAAAGCGGA CATTGTCCAT GCCTTTCTGT CGATCCCCAG CCTGAGTCAC 
AGTGTACTGT CACAGACCAG CTTCAGGGCC GAATACAAGG CCAGCGGCGG CCCCTCCGTC 
TTCCAGAAGC CTGTCCGCTT TCAGGTGGAC ATCAGCTCCT CTGAGGGTCC AGAACCCTCA 
CCCCGAAGAG ATGGCAGCAG TGGAGGTGGC ATCTACTCAG TCACCTTTAC TCTCATCTCC 
GGTCCCAGCC GTCGGTTCAA ACGTGTGGTA GAGACCATCC AGGCACAGTT GCTGAGTACT 
CACGACCAGC CCTCCGTGCA GGCCTTGGCA GATGAGAAGA ACGGAGCCCA GACCCGGCCT 
GCTGGGACCC CACCCCGAAG CCTGCAGCCC CCACCAGGCC GCTCTGACCC TGATCTGAGT 
AGCTCTCCCC GCCGAGGACC CCCTAAGGAC AAGAAGCTCC TGGCCACCAA TGGAACCCCT 
CTACCCTGCC CCCCTACTGT GAATCTGTAT ATAAGGCCCA AGGAGTATGT TGGGAGGGGG 
GACACAAAAC TAGGCCTTGC CCTGCAGGGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure and inhibition analysis of the mouse SAD-B C-terminal fragment.
Bioscience, biotechnology, and biochemistry80(10)1939-46(2016 Oct)
Ma H,Wu JX,Wang J,Wang ZX,Wu JW

SAD-B Phosphorylation of CAST Controls Active Zone Vesicle Recycling for Synaptic Depression.
Cell reports16(11)2901-2913(2016 09)
Mochida S,Hida Y,Tanifuji S,Hagiwara A,Hamada S,Abe M,Ma H,Yasumura M,Kitajima I,Sakimura K,Ohtsuka T

SAD-B kinase regulates pre-synaptic vesicular dynamics at hippocampal Schaffer collateral synapses and affects contextual fear memory.
Journal of neurochemistry136(1)36-47(2016 Jan)
Watabe AM,Nagase M,Hagiwara A,Hida Y,Tsuji M,Ochiai T,Kato F,Ohtsuka T

LKB1 and AMPK regulate synaptic remodeling in old age.
Nature neuroscience17(9)1190-7(2014 Sep)
Samuel MA,Voinescu PE,Lilley BN,de Cabo R,Foretz M,Viollet B,Pawlyk B,Sandberg MA,Vavvas DG,Sanes JR

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Brsk1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

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