Shisa6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Shisa6
Entrez Gene ID	380702
Full Name	shisa family member 6
Synonyms	Gm879,ckamp52
General protein information	Preferred Names shisa family member 6 Names protein shisa-6 homolog cysteine-knot AMPAR-modulating protein 52 shisa homolog 6
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006533619.3	XP_006533682.1	protein shisa-6 homolog isoform X1
XM_006533619.4	XP_006533682.1	protein shisa-6 isoform X1
XM_030246098.1	XP_030101958.1	protein shisa-6 isoform X1
XM_030246099.1	XP_030101959.1	protein shisa-6 isoform X2
XM_030246100.1	XP_030101960.1	protein shisa-6 isoform X3
NM_001034874.3	NP_001030046.1	protein shisa-6 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SHISA6	XP_002695894.1
	LOC722098	XP_001118293.1
Pan troglodytes (chimpanzee)	SHISA6	XP_001153258.2
Mus musculus (house mouse)	Shisa6	NP_001030046.1
Xenopus tropicalis (tropical clawed frog)	shisa6	XP_004918465.1
Rattus norvegicus (Norway rat)	Shisa6	NP_001178851.1
Gallus gallus (chicken)	SHISA6	XP_001231791.2
Homo sapiens (human)	SHISA6	NP_997269.2

Gene Ontology
Bibliography

Related articles in PubMed

SHISA6 Confers Resistance to Differentiation-Promoting Wnt/?-Catenin Signaling in Mouse Spermatogenic Stem Cells.
Tokue M, Ikami K, Mizuno S, Takagi C, Miyagi A, Takada R, Noda C, Kitadate Y, Hara K, Mizuguchi H, Sato T, Taketo MM, Sugiyama F, Ogawa T, Kobayashi S, Ueno N, Takahashi S, Takada S, Yoshida S
Stem cell reports8(3)561-575(2017 03)

Shisa6 traps AMPA receptors at postsynaptic sites and prevents their desensitization during synaptic activity.
Klaassen RV, Stroeder J, Coussen F, Hafner AS, Petersen JD, Renancio C, Schmitz LJ, Normand E, Lodder JC, Rotaru DC, Rao-Ruiz P, Spijker S, Mansvelder HD, Choquet D, Smit AB
Nature communications710682(2016 Mar)

Auxiliary subunits of the CKAMP family differentially modulate AMPA receptor properties.
Farrow P, Khodosevich K, Sapir Y, Schulmann A, Aslam M, Stern-Bach Y, Monyer H, von Engelhardt J
eLife4e09693(2015 Dec)

DeCoN: genome-wide analysis of in vivo transcriptional dynamics during pyramidal neuron fate selection in neocortex.
Molyneaux BJ, Goff LA, Brettler AC, Chen HH, Hrvatin S, Rinn JL, Arlotta P
Neuron85(2)275-288(2015 Jan)

High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes.
Schwenk J, Harmel N, Brechet A, Zolles G, Berkefeld H, M?ller CS, Bildl W, Baehrens D, H?ber B, Kulik A, Kl?cker N, Schulte U, Fakler B
Neuron74(4)621-33(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study proposes a generic mechanism of stem cell regulation in a facultative (or open) niche environment, with which different levels of a cell-autonomous inhibitor (SHISA6, in this case) generates heterogeneous resistance to widely distributed differentiation-promoting extracellular signaling, such as WNTs.
Title: SHISA6 Confers Resistance to Differentiation-Promoting Wnt/?-Catenin Signaling in Mouse Spermatogenic Stem Cells.

Shisa6 prevents synaptically trapped AMPARs from depression at high-frequency synaptic transmission.
Title: Shisa6 traps AMPA receptors at postsynaptic sites and prevents their desensitization during synaptic activity.

This study indicates that AMPAR function is very precisely controlled by the cell-type specific expression of the CKAMP family members 39, 52 and 59.
Title: Auxiliary subunits of the CKAMP family differentially modulate AMPA receptor properties.

The following Shisa6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shisa6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu85823	XM_030246099.1 Latest version!	Mus musculus shisa family member 6 (Shisa6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu85824	XM_030246100.1 Latest version!	Mus musculus shisa family member 6 (Shisa6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu46525	XM_030246098.1 Latest version!	Mus musculus shisa family member 6 (Shisa6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu46525	XM_006533619.4 Latest version!	Mus musculus shisa family member 6 (Shisa6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu12769	NM_001034874.3 Latest version!	Mus musculus shisa family member 6 (Shisa6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OMu46525	XM_006533619.3	Mus musculus shisa family member 6 (Shisa6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Shisa6 ( XM_030246099.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101959.1 Mus musculus shisa family member 6 (Shisa6), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu85823
Clone ID Related Accession (Same CDS sequence)	XM_030246099.1
Accession Version	XM_030246099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein shisa-6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACAT TTCTGGATCA TTGGATGGGC TGTCTTGTAC CATGGCTTTT GGGTGATCGT 
CAGTATAATC ATCCGATCTT AAGCAGCGCT ACCCAGACCC CTACACATGA GAAGCCACGG 
ATGAATAACA TTCTGACGTC GGCCACGGAA CCCTATGACC TCTCCTTCTC ACGCTCTTAC 
CAGAACTTAG CCCACTTGCC TCCATCATAT GAATCTGCAG TGAAAACCAA TCCAAGCAAG 
TACTCGTCTC TGAAGAGGCT AACGGACAAG GAAGCTGATG AGTATTACAT GAGAAGGAGG 
CACCTGCCAG ACCTTGCAGC CCGTGGTACC CTCCCCCTCA ATGTCATCCA GATGTCTCAA 
CAGAAGCCAC TTCCTCGAGA ACGGCCACGC AGGCCCATCA GGGCCATGTC CCAGGACAGG 
GTCTTGTCTC CACGTCGGGG ATTGCCAGAT GAATTCGGCA TGCCCTATGA CCGCATCTTG 
TCTGATGAAC AGCTGCTCTC CACAGAGCGC CTGCACTCCC AGGACCCGTT GCTGTCCCCA 
GAGAGGACAG CCTTCCCGGA GCAGTCGCTG TCGCGGGCCA TCTCGCATAC GGACGTCTTT 
GTGTCCACGC CAGTGCTGGA CCGCTACCGC ATGACCAAGA TGCACTCCCA TCCCAGTGCC 
TCCAATAACT CCTATGCCAC CCTGGGCCAG AGCCAGACAG CAGCCAAGCG CCACGCCTTT 
GCCTCTCGCA GACACAACAC GGTGGAGCAG TTACACTATA TCCCAGGCCA TCACACCTGC 
TACACAGCCA GCAAGACTGA AGTGACCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101959.1
CDS	71..883
Translation	MLTFLDHWMGCLVPWLLGDRQYNHPILSSATQTPTHEKPRMNNILTSATEPYDLSFSRSYQNLAHLPPSYESAVKTNPSKYSSLKRLTDKEADEYYMRRRHLPDLAARGTLPLNVIQMSQQKPLPRERPRRPIRAMSQDRVLSPRRGLPDEFGMPYDRILSDEQLLSTERLHSQDPLLSPERTAFPEQSLSRAISHTDVFVSTPVLDRYRMTKMHSHPSASNNSYATLGQSQTAAKRHAFASRRHNTVEQLHYIPGHHTCYTASKTEVTV

Target ORF information:

RefSeq Version	XM_030246099.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus shisa family member 6 (Shisa6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246099.1

ORF Insert Sequence:

ATGCTGACAT TTCTGGATCA TTGGATGGGC TGTCTTGTAC CATGGCTTTT GGGTGATCGT 
CAGTATAATC ATCCGATCTT AAGCAGCGCT ACCCAGACCC CTACACATGA GAAGCCACGG 
ATGAATAACA TTCTGACGTC GGCCACGGAA CCCTATGACC TCTCCTTCTC ACGCTCTTAC 
CAGAACTTAG CCCACTTGCC TCCATCATAT GAATCTGCAG TGAAAACCAA TCCAAGCAAG 
TACTCGTCTC TGAAGAGGCT AACGGACAAG GAAGCTGATG AGTATTACAT GAGAAGGAGG 
CACCTGCCAG ACCTTGCAGC CCGTGGTACC CTCCCCCTCA ATGTCATCCA GATGTCTCAA 
CAGAAGCCAC TTCCTCGAGA ACGGCCACGC AGGCCCATCA GGGCCATGTC CCAGGACAGG 
GTCTTGTCTC CACGTCGGGG ATTGCCAGAT GAATTCGGCA TGCCCTATGA CCGCATCTTG 
TCTGATGAAC AGCTGCTCTC CACAGAGCGC CTGCACTCCC AGGACCCGTT GCTGTCCCCA 
GAGAGGACAG CCTTCCCGGA GCAGTCGCTG TCGCGGGCCA TCTCGCATAC GGACGTCTTT 
GTGTCCACGC CAGTGCTGGA CCGCTACCGC ATGACCAAGA TGCACTCCCA TCCCAGTGCC 
TCCAATAACT CCTATGCCAC CCTGGGCCAG AGCCAGACAG CAGCCAAGCG CCACGCCTTT 
GCCTCTCGCA GACACAACAC GGTGGAGCAG TTACACTATA TCCCAGGCCA TCACACCTGC 
TACACAGCCA GCAAGACTGA AGTGACCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shisa6 ( XM_030246100.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101960.1 Mus musculus shisa family member 6 (Shisa6), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu85824
Clone ID Related Accession (Same CDS sequence)	XM_030246100.1
Accession Version	XM_030246100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein shisa-6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGC GCCGCCTCCT GCTGCCGCCT CTGCTGCTGT CGCTGCTGCT GTCGCTCGCG 
TCCCTGCACC TGCCGCCCGG CGCAGACGCC GCCCGCGGCC GCTCCGGGAA CCGGACCCTG 
AACGCGGGAG CCGTCGGAGG TCGGCGTGCT GGGGGCGCCC TGGCCCGAGG CGGCCGCGAA 
CTAAACAGCA CCGCCCGAGC GTCCGGCGTC CCGGAGGCGG GCAGCCGGCG GGGACAGTCC 
GCAGCAGCGG CAGCGGCGGC GGCGGCGGCG GCCAGCGCGA CTGTTACTTA CGAGACGTGC 
TGGGGCTACT ACGACGTGAG CGGCCAGTAC GACAAGGAGT TCGAGTGCAA CAACAGCGAG 
AGCGGCTACC TGTACTGCTG CGGCACCTGC TACTATCGCT TCTGCTGCAA GAAGCGCCAC 
GAGAAGCTGG ACCAGCGCCA GTGCACCAAC TACCAAAGCC CCGTATGGGT GCAGACGCCC 
AGCACCAAGG TAGTGTCGCC GGGGCCCGAG AACAAGTACG ACCCGGAGAA GGACAAGACC 
AACTTCACCG TCTACATCAC TTGCGGGGTG ATCGCCTTCG TCATCGTGGC GGGCGTCTTC 
GCCAAGGTCT CCTATGACAA GGCCCACCGC CCTCCGCGAG AGATGAACAT CCACAGGGAG 
ATCATTGGAA AGTCAAAGCC AAAGGGACAA CAGGAGCCCA GCCTCTCCTT TCGGAACTGC 
ACAGGCAGAG GATGCGAAAT CAGCTGTGCT TCCAGCGTCC CCATGAAGAT GAATGGTTGG 
ATGTGGTTCC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101960.1
CDS	189..983
Translation	MALRRLLLPPLLLSLLLSLASLHLPPGADAARGRSGNRTLNAGAVGGRRAGGALARGGRELNSTARASGVPEAGSRRGQSAAAAAAAAAAASATVTYETCWGYYDVSGQYDKEFECNNSESGYLYCCGTCYYRFCCKKRHEKLDQRQCTNYQSPVWVQTPSTKVVSPGPENKYDPEKDKTNFTVYITCGVIAFVIVAGVFAKVSYDKAHRPPREMNIHREIIGKSKPKGQQEPSLSFRNCTGRGCEISCASSVPMKMNGWMWFQ

Target ORF information:

RefSeq Version	XM_030246100.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus shisa family member 6 (Shisa6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246100.1

ORF Insert Sequence:

ATGGCGCTGC GCCGCCTCCT GCTGCCGCCT CTGCTGCTGT CGCTGCTGCT GTCGCTCGCG 
TCCCTGCACC TGCCGCCCGG CGCAGACGCC GCCCGCGGCC GCTCCGGGAA CCGGACCCTG 
AACGCGGGAG CCGTCGGAGG TCGGCGTGCT GGGGGCGCCC TGGCCCGAGG CGGCCGCGAA 
CTAAACAGCA CCGCCCGAGC GTCCGGCGTC CCGGAGGCGG GCAGCCGGCG GGGACAGTCC 
GCAGCAGCGG CAGCGGCGGC GGCGGCGGCG GCCAGCGCGA CTGTTACTTA CGAGACGTGC 
TGGGGCTACT ACGACGTGAG CGGCCAGTAC GACAAGGAGT TCGAGTGCAA CAACAGCGAG 
AGCGGCTACC TGTACTGCTG CGGCACCTGC TACTATCGCT TCTGCTGCAA GAAGCGCCAC 
GAGAAGCTGG ACCAGCGCCA GTGCACCAAC TACCAAAGCC CCGTATGGGT GCAGACGCCC 
AGCACCAAGG TAGTGTCGCC GGGGCCCGAG AACAAGTACG ACCCGGAGAA GGACAAGACC 
AACTTCACCG TCTACATCAC TTGCGGGGTG ATCGCCTTCG TCATCGTGGC GGGCGTCTTC 
GCCAAGGTCT CCTATGACAA GGCCCACCGC CCTCCGCGAG AGATGAACAT CCACAGGGAG 
ATCATTGGAA AGTCAAAGCC AAAGGGACAA CAGGAGCCCA GCCTCTCCTT TCGGAACTGC 
ACAGGCAGAG GATGCGAAAT CAGCTGTGCT TCCAGCGTCC CCATGAAGAT GAATGGTTGG 
ATGTGGTTCC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shisa6 ( XM_006533619.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533682.1 Mus musculus shisa family member 6 (Shisa6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46525
Clone ID Related Accession (Same CDS sequence)	XM_006533619.3 , XM_006533619.4 , XM_030246098.1
Accession Version	XM_006533619.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein shisa-6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533619.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGC GCCGCCTCCT GCTGCCGCCT CTGCTGCTGT CGCTGCTGCT GTCGCTCGCG 
TCCCTGCACC TGCCGCCCGG CGCAGACGCC GCCCGCGGCC GCTCCGGGAA CCGGACCCTG 
AACGCGGGAG CCGTCGGAGG TCGGCGTGCT GGGGGCGCCC TGGCCCGAGG CGGCCGCGAA 
CTAAACAGCA CCGCCCGAGC GTCCGGCGTC CCGGAGGCGG GCAGCCGGCG GGGACAGTCC 
GCAGCAGCGG CAGCGGCGGC GGCGGCGGCG GCCAGCGCGA CTGTTACTTA CGAGACGTGC 
TGGGGCTACT ACGACGTGAG CGGCCAGTAC GACAAGGAGT TCGAGTGCAA CAACAGCGAG 
AGCGGCTACC TGTACTGCTG CGGCACCTGC TACTATCGCT TCTGCTGCAA GAAGCGCCAC 
GAGAAGCTGG ACCAGCGCCA GTGCACCAAC TACCAAAGCC CCGTATGGGT GCAGACGCCC 
AGCACCAAGG TAGTGTCGCC GGGGCCCGAG AACAAGTACG ACCCGGAGAA GGACAAGACC 
AACTTCACCG TCTACATCAC TTGCGGGGTG ATCGCCTTCG TCATCGTGGC GGGCGTCTTC 
GCCAAGGTCT CCTATGACAA GGCCCACCGC CCTCCGCGAG AGATGAACAT CCACAGGGCT 
CTGGCTGACA TTCTAAGACA ACAGGGACCA ATCCCCATAG CACACTGTGA AAGAGAAACC 
ATCTCGGCCA TCGATACCTC TCCCAAAGAG AACACGCCGG TCCGATCAAC CTCCAAAAAC 
CACTACACCC CTGTGCGCAC AGCCAAGCAG ACTCCAGGGC ATTATGGGAA GGATGCTTAC 
CGAAGTGGAG GACCAGATCT CCATAACTTC ATCTCATCTG GATTTGTCAC ATTAGGAAGA 
GGACACACGA AGGGTGATCG TCAGTATAAT CATCCGATCT TAAGCAGCGC TACCCAGACC 
CCTACACATG AGAAGCCACG GATGAATAAC ATTCTGACGT CGGCCACGGA ACCCTATGAC 
CTCTCCTTCT CACGCTCTTA CCAGAACTTA GCCCACTTGC CTCCATCATA TGAATCTGCA 
GTGAAAACCA ATCCAAGCAA GTACTCGTCT CTGAAGAGGC TAACGGACAA GGAAGCTGAT 
GAGTATTACA TGAGAAGGAG GCACCTGCCA GACCTTGCAG CCCGTGGTAC CCTCCCCCTC 
AATGTCATCC AGATGTCTCA ACAGAAGCCA CTTCCTCGAG AACGGCCACG CAGGCCCATC 
AGGGCCATGT CCCAGGACAG GGTCTTGTCT CCACGTCGGG GATTGCCAGA TGAATTCGGC 
ATGCCCTATG ACCGCATCTT GTCTGATGAA CAGCTGCTCT CCACAGAGCG CCTGCACTCC 
CAGGACCCGT TGCTGTCCCC AGAGAGGACA GCCTTCCCGG AGCAGTCGCT GTCGCGGGCC 
ATCTCGCATA CGGACGTCTT TGTGTCCACG CCAGTGCTGG ACCGCTACCG CATGACCAAG 
ATGCACTCCC ATCCCAGTGC CTCCAATAAC TCCTATGCCA CCCTGGGCCA GAGCCAGACA 
GCAGCCAAGC GCCACGCCTT TGCCTCTCGC AGACACAACA CGGTGGAGCA GTTACACTAT 
ATCCCAGGCC ATCACACCTG CTACACAGCC AGCAAGACTG AAGTGACCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533682.1
CDS	587..2260
Translation	MALRRLLLPPLLLSLLLSLASLHLPPGADAARGRSGNRTLNAGAVGGRRAGGALARGGRELNSTARASGVPEAGSRRGQSAAAAAAAAAAASATVTYETCWGYYDVSGQYDKEFECNNSESGYLYCCGTCYYRFCCKKRHEKLDQRQCTNYQSPVWVQTPSTKVVSPGPENKYDPEKDKTNFTVYITCGVIAFVIVAGVFAKVSYDKAHRPPREMNIHRALADILRQQGPIPIAHCERETISAIDTSPKENTPVRSTSKNHYTPVRTAKQTPGHYGKDAYRSGGPDLHNFISSGFVTLGRGHTKGDRQYNHPILSSATQTPTHEKPRMNNILTSATEPYDLSFSRSYQNLAHLPPSYESAVKTNPSKYSSLKRLTDKEADEYYMRRRHLPDLAARGTLPLNVIQMSQQKPLPRERPRRPIRAMSQDRVLSPRRGLPDEFGMPYDRILSDEQLLSTERLHSQDPLLSPERTAFPEQSLSRAISHTDVFVSTPVLDRYRMTKMHSHPSASNNSYATLGQSQTAAKRHAFASRRHNTVEQLHYIPGHHTCYTASKTEVTV

Target ORF information:

RefSeq Version	XM_006533619.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus shisa family member 6 (Shisa6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533619.4

ORF Insert Sequence:

ATGGCGCTGC GCCGCCTCCT GCTGCCGCCT CTGCTGCTGT CGCTGCTGCT GTCGCTCGCG 
TCCCTGCACC TGCCGCCCGG CGCAGACGCC GCCCGCGGCC GCTCCGGGAA CCGGACCCTG 
AACGCGGGAG CCGTCGGAGG TCGGCGTGCT GGGGGCGCCC TGGCCCGAGG CGGCCGCGAA 
CTAAACAGCA CCGCCCGAGC GTCCGGCGTC CCGGAGGCGG GCAGCCGGCG GGGACAGTCC 
GCAGCAGCGG CAGCGGCGGC GGCGGCGGCG GCCAGCGCGA CTGTTACTTA CGAGACGTGC 
TGGGGCTACT ACGACGTGAG CGGCCAGTAC GACAAGGAGT TCGAGTGCAA CAACAGCGAG 
AGCGGCTACC TGTACTGCTG CGGCACCTGC TACTATCGCT TCTGCTGCAA GAAGCGCCAC 
GAGAAGCTGG ACCAGCGCCA GTGCACCAAC TACCAAAGCC CCGTATGGGT GCAGACGCCC 
AGCACCAAGG TAGTGTCGCC GGGGCCCGAG AACAAGTACG ACCCGGAGAA GGACAAGACC 
AACTTCACCG TCTACATCAC TTGCGGGGTG ATCGCCTTCG TCATCGTGGC GGGCGTCTTC 
GCCAAGGTCT CCTATGACAA GGCCCACCGC CCTCCGCGAG AGATGAACAT CCACAGGGCT 
CTGGCTGACA TTCTAAGACA ACAGGGACCA ATCCCCATAG CACACTGTGA AAGAGAAACC 
ATCTCGGCCA TCGATACCTC TCCCAAAGAG AACACGCCGG TCCGATCAAC CTCCAAAAAC 
CACTACACCC CTGTGCGCAC AGCCAAGCAG ACTCCAGGGC ATTATGGGAA GGATGCTTAC 
CGAAGTGGAG GACCAGATCT CCATAACTTC ATCTCATCTG GATTTGTCAC ATTAGGAAGA 
GGACACACGA AGGGTGATCG TCAGTATAAT CATCCGATCT TAAGCAGCGC TACCCAGACC 
CCTACACATG AGAAGCCACG GATGAATAAC ATTCTGACGT CGGCCACGGA ACCCTATGAC 
CTCTCCTTCT CACGCTCTTA CCAGAACTTA GCCCACTTGC CTCCATCATA TGAATCTGCA 
GTGAAAACCA ATCCAAGCAA GTACTCGTCT CTGAAGAGGC TAACGGACAA GGAAGCTGAT 
GAGTATTACA TGAGAAGGAG GCACCTGCCA GACCTTGCAG CCCGTGGTAC CCTCCCCCTC 
AATGTCATCC AGATGTCTCA ACAGAAGCCA CTTCCTCGAG AACGGCCACG CAGGCCCATC 
AGGGCCATGT CCCAGGACAG GGTCTTGTCT CCACGTCGGG GATTGCCAGA TGAATTCGGC 
ATGCCCTATG ACCGCATCTT GTCTGATGAA CAGCTGCTCT CCACAGAGCG CCTGCACTCC 
CAGGACCCGT TGCTGTCCCC AGAGAGGACA GCCTTCCCGG AGCAGTCGCT GTCGCGGGCC 
ATCTCGCATA CGGACGTCTT TGTGTCCACG CCAGTGCTGG ACCGCTACCG CATGACCAAG 
ATGCACTCCC ATCCCAGTGC CTCCAATAAC TCCTATGCCA CCCTGGGCCA GAGCCAGACA 
GCAGCCAAGC GCCACGCCTT TGCCTCTCGC AGACACAACA CGGTGGAGCA GTTACACTAT 
ATCCCAGGCC ATCACACCTG CTACACAGCC AGCAAGACTG AAGTGACCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shisa6 ( XM_030246098.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101958.1 Mus musculus shisa family member 6 (Shisa6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu46525
Clone ID Related Accession (Same CDS sequence)	XM_006533619.3 , XM_006533619.4 , XM_030246098.1
Accession Version	XM_030246098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein shisa-6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGC GCCGCCTCCT GCTGCCGCCT CTGCTGCTGT CGCTGCTGCT GTCGCTCGCG 
TCCCTGCACC TGCCGCCCGG CGCAGACGCC GCCCGCGGCC GCTCCGGGAA CCGGACCCTG 
AACGCGGGAG CCGTCGGAGG TCGGCGTGCT GGGGGCGCCC TGGCCCGAGG CGGCCGCGAA 
CTAAACAGCA CCGCCCGAGC GTCCGGCGTC CCGGAGGCGG GCAGCCGGCG GGGACAGTCC 
GCAGCAGCGG CAGCGGCGGC GGCGGCGGCG GCCAGCGCGA CTGTTACTTA CGAGACGTGC 
TGGGGCTACT ACGACGTGAG CGGCCAGTAC GACAAGGAGT TCGAGTGCAA CAACAGCGAG 
AGCGGCTACC TGTACTGCTG CGGCACCTGC TACTATCGCT TCTGCTGCAA GAAGCGCCAC 
GAGAAGCTGG ACCAGCGCCA GTGCACCAAC TACCAAAGCC CCGTATGGGT GCAGACGCCC 
AGCACCAAGG TAGTGTCGCC GGGGCCCGAG AACAAGTACG ACCCGGAGAA GGACAAGACC 
AACTTCACCG TCTACATCAC TTGCGGGGTG ATCGCCTTCG TCATCGTGGC GGGCGTCTTC 
GCCAAGGTCT CCTATGACAA GGCCCACCGC CCTCCGCGAG AGATGAACAT CCACAGGGCT 
CTGGCTGACA TTCTAAGACA ACAGGGACCA ATCCCCATAG CACACTGTGA AAGAGAAACC 
ATCTCGGCCA TCGATACCTC TCCCAAAGAG AACACGCCGG TCCGATCAAC CTCCAAAAAC 
CACTACACCC CTGTGCGCAC AGCCAAGCAG ACTCCAGGGC ATTATGGGAA GGATGCTTAC 
CGAAGTGGAG GACCAGATCT CCATAACTTC ATCTCATCTG GATTTGTCAC ATTAGGAAGA 
GGACACACGA AGGGTGATCG TCAGTATAAT CATCCGATCT TAAGCAGCGC TACCCAGACC 
CCTACACATG AGAAGCCACG GATGAATAAC ATTCTGACGT CGGCCACGGA ACCCTATGAC 
CTCTCCTTCT CACGCTCTTA CCAGAACTTA GCCCACTTGC CTCCATCATA TGAATCTGCA 
GTGAAAACCA ATCCAAGCAA GTACTCGTCT CTGAAGAGGC TAACGGACAA GGAAGCTGAT 
GAGTATTACA TGAGAAGGAG GCACCTGCCA GACCTTGCAG CCCGTGGTAC CCTCCCCCTC 
AATGTCATCC AGATGTCTCA ACAGAAGCCA CTTCCTCGAG AACGGCCACG CAGGCCCATC 
AGGGCCATGT CCCAGGACAG GGTCTTGTCT CCACGTCGGG GATTGCCAGA TGAATTCGGC 
ATGCCCTATG ACCGCATCTT GTCTGATGAA CAGCTGCTCT CCACAGAGCG CCTGCACTCC 
CAGGACCCGT TGCTGTCCCC AGAGAGGACA GCCTTCCCGG AGCAGTCGCT GTCGCGGGCC 
ATCTCGCATA CGGACGTCTT TGTGTCCACG CCAGTGCTGG ACCGCTACCG CATGACCAAG 
ATGCACTCCC ATCCCAGTGC CTCCAATAAC TCCTATGCCA CCCTGGGCCA GAGCCAGACA 
GCAGCCAAGC GCCACGCCTT TGCCTCTCGC AGACACAACA CGGTGGAGCA GTTACACTAT 
ATCCCAGGCC ATCACACCTG CTACACAGCC AGCAAGACTG AAGTGACCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101958.1
CDS	547..2220
Translation	MALRRLLLPPLLLSLLLSLASLHLPPGADAARGRSGNRTLNAGAVGGRRAGGALARGGRELNSTARASGVPEAGSRRGQSAAAAAAAAAAASATVTYETCWGYYDVSGQYDKEFECNNSESGYLYCCGTCYYRFCCKKRHEKLDQRQCTNYQSPVWVQTPSTKVVSPGPENKYDPEKDKTNFTVYITCGVIAFVIVAGVFAKVSYDKAHRPPREMNIHRALADILRQQGPIPIAHCERETISAIDTSPKENTPVRSTSKNHYTPVRTAKQTPGHYGKDAYRSGGPDLHNFISSGFVTLGRGHTKGDRQYNHPILSSATQTPTHEKPRMNNILTSATEPYDLSFSRSYQNLAHLPPSYESAVKTNPSKYSSLKRLTDKEADEYYMRRRHLPDLAARGTLPLNVIQMSQQKPLPRERPRRPIRAMSQDRVLSPRRGLPDEFGMPYDRILSDEQLLSTERLHSQDPLLSPERTAFPEQSLSRAISHTDVFVSTPVLDRYRMTKMHSHPSASNNSYATLGQSQTAAKRHAFASRRHNTVEQLHYIPGHHTCYTASKTEVTV

Target ORF information:

RefSeq Version	XM_030246098.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus shisa family member 6 (Shisa6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246098.1

ORF Insert Sequence:

ATGGCGCTGC GCCGCCTCCT GCTGCCGCCT CTGCTGCTGT CGCTGCTGCT GTCGCTCGCG 
TCCCTGCACC TGCCGCCCGG CGCAGACGCC GCCCGCGGCC GCTCCGGGAA CCGGACCCTG 
AACGCGGGAG CCGTCGGAGG TCGGCGTGCT GGGGGCGCCC TGGCCCGAGG CGGCCGCGAA 
CTAAACAGCA CCGCCCGAGC GTCCGGCGTC CCGGAGGCGG GCAGCCGGCG GGGACAGTCC 
GCAGCAGCGG CAGCGGCGGC GGCGGCGGCG GCCAGCGCGA CTGTTACTTA CGAGACGTGC 
TGGGGCTACT ACGACGTGAG CGGCCAGTAC GACAAGGAGT TCGAGTGCAA CAACAGCGAG 
AGCGGCTACC TGTACTGCTG CGGCACCTGC TACTATCGCT TCTGCTGCAA GAAGCGCCAC 
GAGAAGCTGG ACCAGCGCCA GTGCACCAAC TACCAAAGCC CCGTATGGGT GCAGACGCCC 
AGCACCAAGG TAGTGTCGCC GGGGCCCGAG AACAAGTACG ACCCGGAGAA GGACAAGACC 
AACTTCACCG TCTACATCAC TTGCGGGGTG ATCGCCTTCG TCATCGTGGC GGGCGTCTTC 
GCCAAGGTCT CCTATGACAA GGCCCACCGC CCTCCGCGAG AGATGAACAT CCACAGGGCT 
CTGGCTGACA TTCTAAGACA ACAGGGACCA ATCCCCATAG CACACTGTGA AAGAGAAACC 
ATCTCGGCCA TCGATACCTC TCCCAAAGAG AACACGCCGG TCCGATCAAC CTCCAAAAAC 
CACTACACCC CTGTGCGCAC AGCCAAGCAG ACTCCAGGGC ATTATGGGAA GGATGCTTAC 
CGAAGTGGAG GACCAGATCT CCATAACTTC ATCTCATCTG GATTTGTCAC ATTAGGAAGA 
GGACACACGA AGGGTGATCG TCAGTATAAT CATCCGATCT TAAGCAGCGC TACCCAGACC 
CCTACACATG AGAAGCCACG GATGAATAAC ATTCTGACGT CGGCCACGGA ACCCTATGAC 
CTCTCCTTCT CACGCTCTTA CCAGAACTTA GCCCACTTGC CTCCATCATA TGAATCTGCA 
GTGAAAACCA ATCCAAGCAA GTACTCGTCT CTGAAGAGGC TAACGGACAA GGAAGCTGAT 
GAGTATTACA TGAGAAGGAG GCACCTGCCA GACCTTGCAG CCCGTGGTAC CCTCCCCCTC 
AATGTCATCC AGATGTCTCA ACAGAAGCCA CTTCCTCGAG AACGGCCACG CAGGCCCATC 
AGGGCCATGT CCCAGGACAG GGTCTTGTCT CCACGTCGGG GATTGCCAGA TGAATTCGGC 
ATGCCCTATG ACCGCATCTT GTCTGATGAA CAGCTGCTCT CCACAGAGCG CCTGCACTCC 
CAGGACCCGT TGCTGTCCCC AGAGAGGACA GCCTTCCCGG AGCAGTCGCT GTCGCGGGCC 
ATCTCGCATA CGGACGTCTT TGTGTCCACG CCAGTGCTGG ACCGCTACCG CATGACCAAG 
ATGCACTCCC ATCCCAGTGC CTCCAATAAC TCCTATGCCA CCCTGGGCCA GAGCCAGACA 
GCAGCCAAGC GCCACGCCTT TGCCTCTCGC AGACACAACA CGGTGGAGCA GTTACACTAT 
ATCCCAGGCC ATCACACCTG CTACACAGCC AGCAAGACTG AAGTGACCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shisa6 ( NM_001034874.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001030046.1 Mus musculus shisa family member 6 (Shisa6), mRNA.

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CloneID	OMu12769
Clone ID Related Accession (Same CDS sequence)	NM_001034874.3
Accession Version	NM_001034874.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	protein shisa-6 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147504.1 and AL663083.5. On Dec 16, 2009 this sequence version replaced NM_001034874.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK147504.1, SRR1660815.116641.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTGC GCCGCCTCCT GCTGCCGCCT CTGCTGCTGT CGCTGCTGCT GTCGCTCGCG 
TCCCTGCACC TGCCGCCCGG CGCAGACGCC GCCCGCGGCC GCTCCGGGAA CCGGACCCTG 
AACGCGGGAG CCGTCGGAGG TCGGCGTGCT GGGGGCGCCC TGGCCCGAGG CGGCCGCGAA 
CTAAACAGCA CCGCCCGAGC GTCCGGCGTC CCGGAGGCGG GCAGCCGGCG GGGACAGTCC 
GCAGCAGCGG CAGCGGCGGC GGCGGCGGCG GCCAGCGCGA CTGTTACTTA CGAGACGTGC 
TGGGGCTACT ACGACGTGAG CGGCCAGTAC GACAAGGAGT TCGAGTGCAA CAACAGCGAG 
AGCGGCTACC TGTACTGCTG CGGCACCTGC TACTATCGCT TCTGCTGCAA GAAGCGCCAC 
GAGAAGCTGG ACCAGCGCCA GTGCACCAAC TACCAAAGCC CCGTATGGGT GCAGACGCCC 
AGCACCAAGG TAGTGTCGCC GGGGCCCGAG AACAAGTACG ACCCGGAGAA GGACAAGACC 
AACTTCACCG TCTACATCAC TTGCGGGGTG ATCGCCTTCG TCATCGTGGC GGGCGTCTTC 
GCCAAGGTCT CCTATGACAA GGCCCACCGC CCTCCGCGAG AGATGAACAT CCACAGGGCT 
CTGGCTGACA TTCTAAGACA ACAGGGACCA ATCCCCATAG CACACTGTGA AAGAGAAACC 
ATCTCGGCCA TCGATACCTC TCCCAAAGAG AACACGCCGG TCCGATCAAC CTCCAAAAAC 
CACTACACCC CTGTGCGCAC AGCCAAGCAG ACTCCAGGTG ATCGTCAGTA TAATCATCCG 
ATCTTAAGCA GCGCTACCCA GACCCCTACA CATGAGAAGC CACGGATGAA TAACATTCTG 
ACGTCGGCCA CGGAACCCTA TGACCTCTCC TTCTCACGCT CTTACCAGAA CTTAGCCCAC 
TTGCCTCCAT CATATGAATC TGCAGTGAAA ACCAATCCAA GCAAGTACTC GTCTCTGAAG 
AGGCTAACGG ACAAGGAAGC TGATGAGTAT TACATGAGAA GGAGGCACCT GCCAGACCTT 
GCAGCCCGTG GTACCCTCCC CCTCAATGTC ATCCAGATGT CTCAACAGAA GCCACTTCCT 
CGAGAACGGC CACGCAGGCC CATCAGGGCC ATGTCCCAGG ACAGGGTCTT GTCTCCACGT 
CGGGGATTGC CAGATGAATT CGGCATGCCC TATGACCGCA TCTTGTCTGA TGAACAGCTG 
CTCTCCACAG AGCGCCTGCA CTCCCAGGAC CCGTTGCTGT CCCCAGAGAG GACAGCCTTC 
CCGGAGCAGT CGCTGTCGCG GGCCATCTCG CATACGGACG TCTTTGTGTC CACGCCAGTG 
CTGGACCGCT ACCGCATGAC CAAGATGCAC TCCCATCCCA GTGCCTCCAA TAACTCCTAT 
GCCACCCTGG GCCAGAGCCA GACAGCAGCC AAGCGCCACG CCTTTGCCTC TCGCAGACAC 
AACACGGTGG AGCAGTTACA CTATATCCCA GGCCATCACA CCTGCTACAC AGCCAGCAAG 
ACTGAAGTGA CCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030046.1
CDS	163..1740
Translation	MALRRLLLPPLLLSLLLSLASLHLPPGADAARGRSGNRTLNAGAVGGRRAGGALARGGRELNSTARASGVPEAGSRRGQSAAAAAAAAAAASATVTYETCWGYYDVSGQYDKEFECNNSESGYLYCCGTCYYRFCCKKRHEKLDQRQCTNYQSPVWVQTPSTKVVSPGPENKYDPEKDKTNFTVYITCGVIAFVIVAGVFAKVSYDKAHRPPREMNIHRALADILRQQGPIPIAHCERETISAIDTSPKENTPVRSTSKNHYTPVRTAKQTPGDRQYNHPILSSATQTPTHEKPRMNNILTSATEPYDLSFSRSYQNLAHLPPSYESAVKTNPSKYSSLKRLTDKEADEYYMRRRHLPDLAARGTLPLNVIQMSQQKPLPRERPRRPIRAMSQDRVLSPRRGLPDEFGMPYDRILSDEQLLSTERLHSQDPLLSPERTAFPEQSLSRAISHTDVFVSTPVLDRYRMTKMHSHPSASNNSYATLGQSQTAAKRHAFASRRHNTVEQLHYIPGHHTCYTASKTEVTV

Target ORF information:

RefSeq Version	NM_001034874.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus shisa family member 6 (Shisa6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034874.3

ORF Insert Sequence:

ATGGCGCTGC GCCGCCTCCT GCTGCCGCCT CTGCTGCTGT CGCTGCTGCT GTCGCTCGCG 
TCCCTGCACC TGCCGCCCGG CGCAGACGCC GCCCGCGGCC GCTCCGGGAA CCGGACCCTG 
AACGCGGGAG CCGTCGGAGG TCGGCGTGCT GGGGGCGCCC TGGCCCGAGG CGGCCGCGAA 
CTAAACAGCA CCGCCCGAGC GTCCGGCGTC CCGGAGGCGG GCAGCCGGCG GGGACAGTCC 
GCAGCAGCGG CAGCGGCGGC GGCGGCGGCG GCCAGCGCGA CTGTTACTTA CGAGACGTGC 
TGGGGCTACT ACGACGTGAG CGGCCAGTAC GACAAGGAGT TCGAGTGCAA CAACAGCGAG 
AGCGGCTACC TGTACTGCTG CGGCACCTGC TACTATCGCT TCTGCTGCAA GAAGCGCCAC 
GAGAAGCTGG ACCAGCGCCA GTGCACCAAC TACCAAAGCC CCGTATGGGT GCAGACGCCC 
AGCACCAAGG TAGTGTCGCC GGGGCCCGAG AACAAGTACG ACCCGGAGAA GGACAAGACC 
AACTTCACCG TCTACATCAC TTGCGGGGTG ATCGCCTTCG TCATCGTGGC GGGCGTCTTC 
GCCAAGGTCT CCTATGACAA GGCCCACCGC CCTCCGCGAG AGATGAACAT CCACAGGGCT 
CTGGCTGACA TTCTAAGACA ACAGGGACCA ATCCCCATAG CACACTGTGA AAGAGAAACC 
ATCTCGGCCA TCGATACCTC TCCCAAAGAG AACACGCCGG TCCGATCAAC CTCCAAAAAC 
CACTACACCC CTGTGCGCAC AGCCAAGCAG ACTCCAGGTG ATCGTCAGTA TAATCATCCG 
ATCTTAAGCA GCGCTACCCA GACCCCTACA CATGAGAAGC CACGGATGAA TAACATTCTG 
ACGTCGGCCA CGGAACCCTA TGACCTCTCC TTCTCACGCT CTTACCAGAA CTTAGCCCAC 
TTGCCTCCAT CATATGAATC TGCAGTGAAA ACCAATCCAA GCAAGTACTC GTCTCTGAAG 
AGGCTAACGG ACAAGGAAGC TGATGAGTAT TACATGAGAA GGAGGCACCT GCCAGACCTT 
GCAGCCCGTG GTACCCTCCC CCTCAATGTC ATCCAGATGT CTCAACAGAA GCCACTTCCT 
CGAGAACGGC CACGCAGGCC CATCAGGGCC ATGTCCCAGG ACAGGGTCTT GTCTCCACGT 
CGGGGATTGC CAGATGAATT CGGCATGCCC TATGACCGCA TCTTGTCTGA TGAACAGCTG 
CTCTCCACAG AGCGCCTGCA CTCCCAGGAC CCGTTGCTGT CCCCAGAGAG GACAGCCTTC 
CCGGAGCAGT CGCTGTCGCG GGCCATCTCG CATACGGACG TCTTTGTGTC CACGCCAGTG 
CTGGACCGCT ACCGCATGAC CAAGATGCAC TCCCATCCCA GTGCCTCCAA TAACTCCTAT 
GCCACCCTGG GCCAGAGCCA GACAGCAGCC AAGCGCCACG CCTTTGCCTC TCGCAGACAC 
AACACGGTGG AGCAGTTACA CTATATCCCA GGCCATCACA CCTGCTACAC AGCCAGCAAG 
ACTGAAGTGA CCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shisa6 ( XM_006533619.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533682.1 Mus musculus shisa family member 6 (Shisa6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46525
Clone ID Related Accession (Same CDS sequence)	XM_006533619.3 , XM_006533619.4 , XM_030246098.1
Accession Version	XM_006533619.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein shisa-6 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533619.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGC GCCGCCTCCT GCTGCCGCCT CTGCTGCTGT CGCTGCTGCT GTCGCTCGCG 
TCCCTGCACC TGCCGCCCGG CGCAGACGCC GCCCGCGGCC GCTCCGGGAA CCGGACCCTG 
AACGCGGGAG CCGTCGGAGG TCGGCGTGCT GGGGGCGCCC TGGCCCGAGG CGGCCGCGAA 
CTAAACAGCA CCGCCCGAGC GTCCGGCGTC CCGGAGGCGG GCAGCCGGCG GGGACAGTCC 
GCAGCAGCGG CAGCGGCGGC GGCGGCGGCG GCCAGCGCGA CTGTTACTTA CGAGACGTGC 
TGGGGCTACT ACGACGTGAG CGGCCAGTAC GACAAGGAGT TCGAGTGCAA CAACAGCGAG 
AGCGGCTACC TGTACTGCTG CGGCACCTGC TACTATCGCT TCTGCTGCAA GAAGCGCCAC 
GAGAAGCTGG ACCAGCGCCA GTGCACCAAC TACCAAAGCC CCGTATGGGT GCAGACGCCC 
AGCACCAAGG TAGTGTCGCC GGGGCCCGAG AACAAGTACG ACCCGGAGAA GGACAAGACC 
AACTTCACCG TCTACATCAC TTGCGGGGTG ATCGCCTTCG TCATCGTGGC GGGCGTCTTC 
GCCAAGGTCT CCTATGACAA GGCCCACCGC CCTCCGCGAG AGATGAACAT CCACAGGGCT 
CTGGCTGACA TTCTAAGACA ACAGGGACCA ATCCCCATAG CACACTGTGA AAGAGAAACC 
ATCTCGGCCA TCGATACCTC TCCCAAAGAG AACACGCCGG TCCGATCAAC CTCCAAAAAC 
CACTACACCC CTGTGCGCAC AGCCAAGCAG ACTCCAGGGC ATTATGGGAA GGATGCTTAC 
CGAAGTGGAG GACCAGATCT CCATAACTTC ATCTCATCTG GATTTGTCAC ATTAGGAAGA 
GGACACACGA AGGGTGATCG TCAGTATAAT CATCCGATCT TAAGCAGCGC TACCCAGACC 
CCTACACATG AGAAGCCACG GATGAATAAC ATTCTGACGT CGGCCACGGA ACCCTATGAC 
CTCTCCTTCT CACGCTCTTA CCAGAACTTA GCCCACTTGC CTCCATCATA TGAATCTGCA 
GTGAAAACCA ATCCAAGCAA GTACTCGTCT CTGAAGAGGC TAACGGACAA GGAAGCTGAT 
GAGTATTACA TGAGAAGGAG GCACCTGCCA GACCTTGCAG CCCGTGGTAC CCTCCCCCTC 
AATGTCATCC AGATGTCTCA ACAGAAGCCA CTTCCTCGAG AACGGCCACG CAGGCCCATC 
AGGGCCATGT CCCAGGACAG GGTCTTGTCT CCACGTCGGG GATTGCCAGA TGAATTCGGC 
ATGCCCTATG ACCGCATCTT GTCTGATGAA CAGCTGCTCT CCACAGAGCG CCTGCACTCC 
CAGGACCCGT TGCTGTCCCC AGAGAGGACA GCCTTCCCGG AGCAGTCGCT GTCGCGGGCC 
ATCTCGCATA CGGACGTCTT TGTGTCCACG CCAGTGCTGG ACCGCTACCG CATGACCAAG 
ATGCACTCCC ATCCCAGTGC CTCCAATAAC TCCTATGCCA CCCTGGGCCA GAGCCAGACA 
GCAGCCAAGC GCCACGCCTT TGCCTCTCGC AGACACAACA CGGTGGAGCA GTTACACTAT 
ATCCCAGGCC ATCACACCTG CTACACAGCC AGCAAGACTG AAGTGACCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533682.1
CDS	158..1831
Translation	MALRRLLLPPLLLSLLLSLASLHLPPGADAARGRSGNRTLNAGAVGGRRAGGALARGGRELNSTARASGVPEAGSRRGQSAAAAAAAAAAASATVTYETCWGYYDVSGQYDKEFECNNSESGYLYCCGTCYYRFCCKKRHEKLDQRQCTNYQSPVWVQTPSTKVVSPGPENKYDPEKDKTNFTVYITCGVIAFVIVAGVFAKVSYDKAHRPPREMNIHRALADILRQQGPIPIAHCERETISAIDTSPKENTPVRSTSKNHYTPVRTAKQTPGHYGKDAYRSGGPDLHNFISSGFVTLGRGHTKGDRQYNHPILSSATQTPTHEKPRMNNILTSATEPYDLSFSRSYQNLAHLPPSYESAVKTNPSKYSSLKRLTDKEADEYYMRRRHLPDLAARGTLPLNVIQMSQQKPLPRERPRRPIRAMSQDRVLSPRRGLPDEFGMPYDRILSDEQLLSTERLHSQDPLLSPERTAFPEQSLSRAISHTDVFVSTPVLDRYRMTKMHSHPSASNNSYATLGQSQTAAKRHAFASRRHNTVEQLHYIPGHHTCYTASKTEVTV

Target ORF information:

RefSeq Version	XM_006533619.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus shisa family member 6 (Shisa6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533619.3

ORF Insert Sequence:

ATGGCGCTGC GCCGCCTCCT GCTGCCGCCT CTGCTGCTGT CGCTGCTGCT GTCGCTCGCG 
TCCCTGCACC TGCCGCCCGG CGCAGACGCC GCCCGCGGCC GCTCCGGGAA CCGGACCCTG 
AACGCGGGAG CCGTCGGAGG TCGGCGTGCT GGGGGCGCCC TGGCCCGAGG CGGCCGCGAA 
CTAAACAGCA CCGCCCGAGC GTCCGGCGTC CCGGAGGCGG GCAGCCGGCG GGGACAGTCC 
GCAGCAGCGG CAGCGGCGGC GGCGGCGGCG GCCAGCGCGA CTGTTACTTA CGAGACGTGC 
TGGGGCTACT ACGACGTGAG CGGCCAGTAC GACAAGGAGT TCGAGTGCAA CAACAGCGAG 
AGCGGCTACC TGTACTGCTG CGGCACCTGC TACTATCGCT TCTGCTGCAA GAAGCGCCAC 
GAGAAGCTGG ACCAGCGCCA GTGCACCAAC TACCAAAGCC CCGTATGGGT GCAGACGCCC 
AGCACCAAGG TAGTGTCGCC GGGGCCCGAG AACAAGTACG ACCCGGAGAA GGACAAGACC 
AACTTCACCG TCTACATCAC TTGCGGGGTG ATCGCCTTCG TCATCGTGGC GGGCGTCTTC 
GCCAAGGTCT CCTATGACAA GGCCCACCGC CCTCCGCGAG AGATGAACAT CCACAGGGCT 
CTGGCTGACA TTCTAAGACA ACAGGGACCA ATCCCCATAG CACACTGTGA AAGAGAAACC 
ATCTCGGCCA TCGATACCTC TCCCAAAGAG AACACGCCGG TCCGATCAAC CTCCAAAAAC 
CACTACACCC CTGTGCGCAC AGCCAAGCAG ACTCCAGGGC ATTATGGGAA GGATGCTTAC 
CGAAGTGGAG GACCAGATCT CCATAACTTC ATCTCATCTG GATTTGTCAC ATTAGGAAGA 
GGACACACGA AGGGTGATCG TCAGTATAAT CATCCGATCT TAAGCAGCGC TACCCAGACC 
CCTACACATG AGAAGCCACG GATGAATAAC ATTCTGACGT CGGCCACGGA ACCCTATGAC 
CTCTCCTTCT CACGCTCTTA CCAGAACTTA GCCCACTTGC CTCCATCATA TGAATCTGCA 
GTGAAAACCA ATCCAAGCAA GTACTCGTCT CTGAAGAGGC TAACGGACAA GGAAGCTGAT 
GAGTATTACA TGAGAAGGAG GCACCTGCCA GACCTTGCAG CCCGTGGTAC CCTCCCCCTC 
AATGTCATCC AGATGTCTCA ACAGAAGCCA CTTCCTCGAG AACGGCCACG CAGGCCCATC 
AGGGCCATGT CCCAGGACAG GGTCTTGTCT CCACGTCGGG GATTGCCAGA TGAATTCGGC 
ATGCCCTATG ACCGCATCTT GTCTGATGAA CAGCTGCTCT CCACAGAGCG CCTGCACTCC 
CAGGACCCGT TGCTGTCCCC AGAGAGGACA GCCTTCCCGG AGCAGTCGCT GTCGCGGGCC 
ATCTCGCATA CGGACGTCTT TGTGTCCACG CCAGTGCTGG ACCGCTACCG CATGACCAAG 
ATGCACTCCC ATCCCAGTGC CTCCAATAAC TCCTATGCCA CCCTGGGCCA GAGCCAGACA 
GCAGCCAAGC GCCACGCCTT TGCCTCTCGC AGACACAACA CGGTGGAGCA GTTACACTAT 
ATCCCAGGCC ATCACACCTG CTACACAGCC AGCAAGACTG AAGTGACCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SHISA6 Confers Resistance to Differentiation-Promoting Wnt/?-Catenin Signaling in Mouse Spermatogenic Stem Cells.
Stem cell reports8(3)561-575(2017 03)
Tokue M,Ikami K,Mizuno S,Takagi C,Miyagi A,Takada R,Noda C,Kitadate Y,Hara K,Mizuguchi H,Sato T,Taketo MM,Sugiyama F,Ogawa T,Kobayashi S,Ueno N,Takahashi S,Takada S,Yoshida S

Shisa6 traps AMPA receptors at postsynaptic sites and prevents their desensitization during synaptic activity.
Nature communications710682(2016 Mar)
Klaassen RV,Stroeder J,Coussen F,Hafner AS,Petersen JD,Renancio C,Schmitz LJ,Normand E,Lodder JC,Rotaru DC,Rao-Ruiz P,Spijker S,Mansvelder HD,Choquet D,Smit AB

Auxiliary subunits of the CKAMP family differentially modulate AMPA receptor properties.
eLife4e09693(2015 Dec)
Farrow P,Khodosevich K,Sapir Y,Schulmann A,Aslam M,Stern-Bach Y,Monyer H,von Engelhardt J

DeCoN: genome-wide analysis of in vivo transcriptional dynamics during pyramidal neuron fate selection in neocortex.
Neuron85(2)275-288(2015 Jan)
Molyneaux BJ,Goff LA,Brettler AC,Chen HH,Hrvatin S,Rinn JL,Arlotta P

High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes.
Neuron74(4)621-33(2012 May)
Schwenk J,Harmel N,Brechet A,Zolles G,Berkefeld H,M?ller CS,Bildl W,Baehrens D,H?ber B,Kulik A,Kl?cker N,Schulte U,Fakler B

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Shisa6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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