Pramel7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pramel7
Entrez Gene ID	347712
Full Name	preferentially expressed antigen in melanoma like 7
Synonyms	AA675346,C81180
General protein information	Preferred Names preferentially expressed antigen in melanoma like 7 Names preferentially expressed antigen in melanoma-like protein 7 prame-like 7
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 D

mRNA and Protein(s)

mRNA	Protein	Name
NM_178250.2	NP_839981.1	preferentially expressed antigen in melanoma-like protein 7

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pramel7	NP_839981.1
Rattus norvegicus (Norway rat)	Pramel7	XP_578142.2

Gene Ontology
Bibliography

Related articles in PubMed

Pramel7 mediates ground-state pluripotency through proteasomal-epigenetic combined pathways.
Graf U, Casanova EA, Wyck S, Dalcher D, Gatti M, Vollenweider E, Okoniewski MJ, Weber FA, Patel SS, Schmid MW, Li J, Sharif J, Wanner GA, Koseki H, Wong J, Pelczar P, Penengo L, Santoro R, Cinelli P
Nature cell biology19(7)763-773(2017 Jul)

Pramel7 mediates LIF/STAT3-dependent self-renewal in embryonic stem cells.
Casanova EA, Shakhova O, Patel SS, Asner IN, Pelczar P, Weber FA, Graf U, Sommer L, B?rki K, Cinelli P
Stem cells (Dayton, Ohio)29(3)474-85(2011 Mar)

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
Tang F, Barbacioru C, Nordman E, Bao S, Lee C, Wang X, Tuch BB, Heard E, Lao K, Surani MA
PloS one6(6)e21208(2011)

Expression profiling in transgenic FVB/N embryonic stem cells overexpressing STAT3.
Cinelli P, Casanova EA, Uhlig S, Lochmatter P, Matsuda T, Yokota T, R?licke T, Ledermann B, B?rki K
BMC developmental biology857(2008 May)

Mbd3, a component of the NuRD co-repressor complex, is required for development of pluripotent cells.
Kaji K, Nichols J, Hendrich B
Development (Cambridge, England)134(6)1123-32(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pramel7 (PRAME-like 7), a protein highly expressed in the inner cell mass but expressed at low levels in embryonic stem cells, targets proteasomal degradation of UHRF1, a key factor for DNA methylation maintenance.
Title: Pramel7 mediates ground-state pluripotency through proteasomal-epigenetic combined pathways.

Pramel7 represents a central and essential factor in the signaling network regulating pluripotency and self-renewal in embryonic stem cells.
Title: Pramel7 mediates LIF/STAT3-dependent self-renewal in embryonic stem cells.

The following Pramel7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pramel7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07155	NM_178250.2 Latest version!	Mus musculus preferentially expressed antigen in melanoma like 7 (Pramel7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$286.30 $409.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pramel7 ( NM_178250.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_839981.1 Mus musculus preferentially expressed antigen in melanoma like 7 (Pramel7), mRNA.

Price & Availability↑

CloneID	OMu07155
Clone ID Related Accession (Same CDS sequence)	NM_178250.2
Accession Version	NM_178250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	preferentially expressed antigen in melanoma-like protein 7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF490343.1. On Jun 3, 2003 this sequence version replaced NM_178250.1. ##Evidence-Data-START## Transcript exon combination :: AF490343.1, BC052825.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849384, SAMN01164137 [ECO:0000350] ##Evidence-Data-END##

ATGGTCAGTG CCCCACCCAC ACTCCAGGAT CAGGCTTTCA GGAGCCTGGT GAGGAATGAA 
GTATTGACCG TCTCTGATGC GGAGTGCCTA ATAAGGGAAT TCTTCCCACC TCTGTTCAAG 
GAGGCCAGCA CTCAAAAAAA ACCAAAGACA ATTATGATCC TGGTGGAGCA CTGGCCCTAC 
CCTTGCCTTC ATGTTGGGTT GTTGATTGAT AAACCCAACT TTCAGATCTT CCAGGCTATA 
CTGGATGGAG TAGACACATG GCTGAAACGG AAGTATCGCC CCAGGATGGG AAGGCTTAAA 
AAGGTTGACT TCAGGGATGC ACAGCATCAT GCTTCCTTAG ATATGCAGGA TGAGAGAGAG 
GGTAGAGACT ACTTAGTAGG AACTTTGCCT AAGAAGCAAA TAGTGGAGGA CCACTCAAGA 
ACAAGGAAAG AGCGTTTGAA GCTGTTCCAT GACCTTTCCT TTATGTCTTC TCTGCATGAG 
GATAAACACC AAACACTGTT ATTGGAGTGG GCAAAGGAAA GGACGAGTTT CCTGCATCTG 
TGCTGTGAGA AGTTGGAGAT AGGAGCAGTA GAAGTGTCTA AGGTCAGGAA TGTATTAAAA 
TTTCTACAGC CAGAGCTCAT CAAGGAGTTG AAACTGAATA CAGTGGGGAA TCTGTCCAAA 
CTGGCAAAAT TCGTACCTTT CATTAGAAAG ATGAGAAACC TTCAGAAACT CATGCTAGTA 
CGCACCTTTG GAACAAGGAC TTTCACTCAA GAGGAGAAGC AGAACATCAG CAAGATCATT 
TCTCTGTTCT GCAAACTCAG CTGTCTCCGG CATCTCACTA TTGATGATGT CTATTTTCTC 
ACAGATCAGA TGAAAGAGTT GCTCAGGTGT CTGGAGGCCC CATTGGTGTC CCTCAAGATC 
ACCCTGTGCC AGCTCTCTCA GTCAGACTTG GAGTCATTTG CCCAGCGCTG GAACTACAGT 
CAGCTTAAAC ATCTATGCTT GAGAGGTGTC ACTTTAACTA ACCTAGATGT CACGCCTCTA 
AGAGATTTCT TAAAGCGTGT TGCAGCTAAC CTGCAGACTC TGGACTTAGA AGACTGTAGG 
ATGGATGATT CTCACTTCAG GACCCTTCTT CCTGCCCTGA TCAAATGCAC CCAGCTCACT 
AGCATCAATT TGTATGACAA TGACATCTCC GAGGATGTCC TGGAGAACTT TTTGCACCGC 
ACTACCAATC TGAGCCAGCT GACCACAGAA ATGTATCCTG CTCCATCAGA GGTCTATAAT 
GAGTCCAACT ATGTTATAGT AGAGATATTT ATTCAGATTT GTTCTGAACT TATGAACAAA 
CTCATGGAAG TCAGGCAGGC CAATTCAGTG TGCTTTGGTT CCTCTTCCTG TTATGACTGT 
GATAATCGTT ATTTATATGA AGATGATGGG GATGTCACAC TTTGTTTGTG CCAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_839981.1
CDS	1..1440
Translation	MVSAPPTLQDQAFRSLVRNEVLTVSDAECLIREFFPPLFKEASTQKKPKTIMILVEHWPYPCLHVGLLIDKPNFQIFQAILDGVDTWLKRKYRPRMGRLKKVDFRDAQHHASLDMQDEREGRDYLVGTLPKKQIVEDHSRTRKERLKLFHDLSFMSSLHEDKHQTLLLEWAKERTSFLHLCCEKLEIGAVEVSKVRNVLKFLQPELIKELKLNTVGNLSKLAKFVPFIRKMRNLQKLMLVRTFGTRTFTQEEKQNISKIISLFCKLSCLRHLTIDDVYFLTDQMKELLRCLEAPLVSLKITLCQLSQSDLESFAQRWNYSQLKHLCLRGVTLTNLDVTPLRDFLKRVAANLQTLDLEDCRMDDSHFRTLLPALIKCTQLTSINLYDNDISEDVLENFLHRTTNLSQLTTEMYPAPSEVYNESNYVIVEIFIQICSELMNKLMEVRQANSVCFGSSSCYDCDNRYLYEDDGDVTLCLCQE

Target ORF information:

RefSeq Version	NM_178250.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus preferentially expressed antigen in melanoma like 7 (Pramel7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178250.2

ORF Insert Sequence:

ATGGTCAGTG CCCCACCCAC ACTCCAGGAT CAGGCTTTCA GGAGCCTGGT GAGGAATGAA 
GTATTGACCG TCTCTGATGC GGAGTGCCTA ATAAGGGAAT TCTTCCCACC TCTGTTCAAG 
GAGGCCAGCA CTCAAAAAAA ACCAAAGACA ATTATGATCC TGGTGGAGCA CTGGCCCTAC 
CCTTGCCTTC ATGTTGGGTT GTTGATTGAT AAACCCAACT TTCAGATCTT CCAGGCTATA 
CTGGATGGAG TAGACACATG GCTGAAACGG AAGTATCGCC CCAGGATGGG AAGGCTTAAA 
AAGGTTGACT TCAGGGATGC ACAGCATCAT GCTTCCTTAG ATATGCAGGA TGAGAGAGAG 
GGTAGAGACT ACTTAGTAGG AACTTTGCCT AAGAAGCAAA TAGTGGAGGA CCACTCAAGA 
ACAAGGAAAG AGCGTTTGAA GCTGTTCCAT GACCTTTCCT TTATGTCTTC TCTGCATGAG 
GATAAACACC AAACACTGTT ATTGGAGTGG GCAAAGGAAA GGACGAGTTT CCTGCATCTG 
TGCTGTGAGA AGTTGGAGAT AGGAGCAGTA GAAGTGTCTA AGGTCAGGAA TGTATTAAAA 
TTTCTACAGC CAGAGCTCAT CAAGGAGTTG AAACTGAATA CAGTGGGGAA TCTGTCCAAA 
CTGGCAAAAT TCGTACCTTT CATTAGAAAG ATGAGAAACC TTCAGAAACT CATGCTAGTA 
CGCACCTTTG GAACAAGGAC TTTCACTCAA GAGGAGAAGC AGAACATCAG CAAGATCATT 
TCTCTGTTCT GCAAACTCAG CTGTCTCCGG CATCTCACTA TTGATGATGT CTATTTTCTC 
ACAGATCAGA TGAAAGAGTT GCTCAGGTGT CTGGAGGCCC CATTGGTGTC CCTCAAGATC 
ACCCTGTGCC AGCTCTCTCA GTCAGACTTG GAGTCATTTG CCCAGCGCTG GAACTACAGT 
CAGCTTAAAC ATCTATGCTT GAGAGGTGTC ACTTTAACTA ACCTAGATGT CACGCCTCTA 
AGAGATTTCT TAAAGCGTGT TGCAGCTAAC CTGCAGACTC TGGACTTAGA AGACTGTAGG 
ATGGATGATT CTCACTTCAG GACCCTTCTT CCTGCCCTGA TCAAATGCAC CCAGCTCACT 
AGCATCAATT TGTATGACAA TGACATCTCC GAGGATGTCC TGGAGAACTT TTTGCACCGC 
ACTACCAATC TGAGCCAGCT GACCACAGAA ATGTATCCTG CTCCATCAGA GGTCTATAAT 
GAGTCCAACT ATGTTATAGT AGAGATATTT ATTCAGATTT GTTCTGAACT TATGAACAAA 
CTCATGGAAG TCAGGCAGGC CAATTCAGTG TGCTTTGGTT CCTCTTCCTG TTATGACTGT 
GATAATCGTT ATTTATATGA AGATGATGGG GATGTCACAC TTTGTTTGTG CCAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pramel7 mediates ground-state pluripotency through proteasomal-epigenetic combined pathways.
Nature cell biology19(7)763-773(2017 Jul)
Graf U,Casanova EA,Wyck S,Dalcher D,Gatti M,Vollenweider E,Okoniewski MJ,Weber FA,Patel SS,Schmid MW,Li J,Sharif J,Wanner GA,Koseki H,Wong J,Pelczar P,Penengo L,Santoro R,Cinelli P

Pramel7 mediates LIF/STAT3-dependent self-renewal in embryonic stem cells.
Stem cells (Dayton, Ohio)29(3)474-85(2011 Mar)
Casanova EA,Shakhova O,Patel SS,Asner IN,Pelczar P,Weber FA,Graf U,Sommer L,B?rki K,Cinelli P

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
PloS one6(6)e21208(2011)
Tang F,Barbacioru C,Nordman E,Bao S,Lee C,Wang X,Tuch BB,Heard E,Lao K,Surani MA

Expression profiling in transgenic FVB/N embryonic stem cells overexpressing STAT3.
BMC developmental biology857(2008 May)
Cinelli P,Casanova EA,Uhlig S,Lochmatter P,Matsuda T,Yokota T,R?licke T,Ledermann B,B?rki K

Mbd3, a component of the NuRD co-repressor complex, is required for development of pluripotent cells.
Development (Cambridge, England)134(6)1123-32(2007 Mar)
Kaji K,Nichols J,Hendrich B