Gcat cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gcat
Entrez Gene ID	26912
Full Name	glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase)
Synonyms	AI526977,Kbl
General protein information	Preferred Names glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) Names 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial AKB ligase aminoacetone synthase glycine acetyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 E1\|15 37.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013847.4	NP_038875.3	2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial isoform a
NM_001161712.1	NP_001155184.1	2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial isoform b

Pathways from BioSystems

Homologies

	GCAT	XP_001089366.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005781	XP_315797.4
Oryza sativa (rice)	Os01g0736400	NP_001044174.2
Bos taurus (cattle)	GCAT	NP_001068602.1
Mus musculus (house mouse)	Gcat	NP_038875.3
Caenorhabditis elegans (roundworm)	T25B9.1	NP_501991.2
Xenopus tropicalis (tropical clawed frog)	gcat	NP_989284.1
Homo sapiens (human)	GCAT	NP_055106.1
Rattus norvegicus (Norway rat)	Gcat	NP_001019448.1
Danio rerio (zebrafish)	gcat	NP_001166025.1
Arabidopsis thaliana (thale cress)	BIOF	NP_974731.1
Pan troglodytes (chimpanzee)	GCAT	XP_515122.2
Canis lupus familiaris (dog)	GCAT	XP_538383.2
Oryza sativa (rice)	Os10g0560900	NP_001065381.1
Drosophila melanogaster (fruit fly)	CG10361	NP_648509.1

Gene Ontology
Bibliography

Related articles in PubMed

Mice deficient in the Shmt2 gene have mitochondrial respiration defects and are embryonic lethal.
Tani H, Ohnishi S, Shitara H, Mito T, Yamaguchi M, Yonekawa H, Hashizume O, Ishikawa K, Nakada K, Hayashi JI
Scientific reports8(1)425(2018 Jan)

Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase by cold and osmotic stress.
Hoshino A, Fujii H
Cell stress & chaperones12(2)186-91(2007)

Proteomic analysis of the mouse liver mitochondrial inner membrane.
Da Cruz S, Xenarios I, Langridge J, Vilbois F, Parone PA, Martinou JC
The Journal of biological chemistry278(42)41566-71(2003 Oct)

Molecular cloning of the human and murine 2-amino-3-ketobutyrate coenzyme A ligase cDNAs.
Edgar AJ, Polak JM
European journal of biochemistry267(6)1805-12(2000 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase (KBL) was detected in response to cold and osmotic stresses.
Title: Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase by cold and osmotic stress.

The following Gcat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gcat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13453	NM_001161712.1 Latest version!	Mus musculus glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) (Gcat), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu16202	NM_013847.4 Latest version!	Mus musculus glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) (Gcat), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gcat ( NM_001161712.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001155184.1 Mus musculus glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) (Gcat), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13453
Clone ID Related Accession (Same CDS sequence)	NM_001161712.1
Accession Version	NM_001161712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Mus musculus(house mouse)
Product	2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL589670.21, AK076469.1 and AK013138.1. Transcript Variant: This variant (2) uses an alternate 3' exon and differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting protein (isoform b) has a shorter and distinct C-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AK076469.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTGGGCCA GCTTCATGTG GCACGGGGCG CTGTCCCCTG GCCGCCGCGC ACATTCAGCG 
CTGGCTCAGT TGCGCTGCAT CCTGGACAGC GAACTGGAAG GGATCCGCGG AGCCGGCACC 
TGGAAGAGTG AGCGTGTGAT CACGTCCCGC CAGGGACCGA GCATCCGCGT GGACGGCATC 
TCGGGAGGAA TCCTCAACTT CTGTGCCAAT AACTACCTGG GCCTGAGCAG CCACCCTGCA 
GTGATCCAGG CAGGTCTGCA GACTCTGGAG GAGTTTGGAG CTGGACTCAG TTCTACTCGA 
TTTATCTGTG GGACCCAGAG CATCCATAAG AATCTAGAAG CCAAGATAGC CCACTTCCAC 
CAGCGTGAGG ACGCCATCCT CTATCCCAGC TGTTTCGATG CCAACGCTGG CCTATTTGAG 
GCTTTGCTGA CCCCAGAGGA TGCGGTTCTG TCAGATGAGC TGAACCATGC TTCCATCATT 
GACGGCATCC GACTGTGCAA GGCCCACAAG TACCGTTACC GCCACCTGGA CATGGCTGAC 
CTGGAAGCCA AGCTGAAGGA GGCTCAGAAG CACAGGCTGC GCCTGGTGGC CACAGATGGA 
GCTTTCTCCA TGGATGGTGA CATCGCTCCC CTACAGGACA TCTGCCGCCT TGCCGCCCAA 
TATGGTGCCC TGGTCTTTGT GGATGAATGC CATGCCACTG GCTTTCTTGG GCCCACAGGA 
CGGGGCACAG ATGAGCTGCT AGGTGTCATG GACCAGGTCA CCATCATCAA CTCCACCCTG 
GGGAAGGCGC TGGGTGGAGC ATCAGGGGGA TATACCACCG GGCCTGAGCC ACTGGTGTCC 
CTGCTACGGC AGCGTTCTCG GCCCTACCTC TTCTCCAACA GCCTGCCCCC TGCTGTAGTC 
GGCTGTGCTT CTAAGGCCCT GGACCTGCTC ATGGAGAGCA ATGCCATCAT CCAGTCTATG 
GCTGCCAAGA CCCGGCGGTT CCGCAGTAAG ATGGAAGCTG CTGGTTTTAC TGTCTCAGGA 
GCCGATCACC CCATCTGCCC TGTGATGCTG GGAGATGCAC GGTTGTCTTC TCAAATGGCA 
GATGACATGT TAAAGAAAGG AAAATGGCTG ACATCCAGAA CATTTCGCTG GACAGCCCAG 
GGAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155184.1
CDS	61..1209
Translation	MWASFMWHGALSPGRRAHSALAQLRCILDSELEGIRGAGTWKSERVITSRQGPSIRVDGISGGILNFCANNYLGLSSHPAVIQAGLQTLEEFGAGLSSTRFICGTQSIHKNLEAKIAHFHQREDAILYPSCFDANAGLFEALLTPEDAVLSDELNHASIIDGIRLCKAHKYRYRHLDMADLEAKLKEAQKHRLRLVATDGAFSMDGDIAPLQDICRLAAQYGALVFVDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTTGPEPLVSLLRQRSRPYLFSNSLPPAVVGCASKALDLLMESNAIIQSMAAKTRRFRSKMEAAGFTVSGADHPICPVMLGDARLSSQMADDMLKKGKWLTSRTFRWTAQGA

Target ORF information:

RefSeq Version	NM_001161712.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) (Gcat), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161712.1

ORF Insert Sequence:

ATGTGGGCCA GCTTCATGTG GCACGGGGCG CTGTCCCCTG GCCGCCGCGC ACATTCAGCG 
CTGGCTCAGT TGCGCTGCAT CCTGGACAGC GAACTGGAAG GGATCCGCGG AGCCGGCACC 
TGGAAGAGTG AGCGTGTGAT CACGTCCCGC CAGGGACCGA GCATCCGCGT GGACGGCATC 
TCGGGAGGAA TCCTCAACTT CTGTGCCAAT AACTACCTGG GCCTGAGCAG CCACCCTGCA 
GTGATCCAGG CAGGTCTGCA GACTCTGGAG GAGTTTGGAG CTGGACTCAG TTCTACTCGA 
TTTATCTGTG GGACCCAGAG CATCCATAAG AATCTAGAAG CCAAGATAGC CCACTTCCAC 
CAGCGTGAGG ACGCCATCCT CTATCCCAGC TGTTTCGATG CCAACGCTGG CCTATTTGAG 
GCTTTGCTGA CCCCAGAGGA TGCGGTTCTG TCAGATGAGC TGAACCATGC TTCCATCATT 
GACGGCATCC GACTGTGCAA GGCCCACAAG TACCGTTACC GCCACCTGGA CATGGCTGAC 
CTGGAAGCCA AGCTGAAGGA GGCTCAGAAG CACAGGCTGC GCCTGGTGGC CACAGATGGA 
GCTTTCTCCA TGGATGGTGA CATCGCTCCC CTACAGGACA TCTGCCGCCT TGCCGCCCAA 
TATGGTGCCC TGGTCTTTGT GGATGAATGC CATGCCACTG GCTTTCTTGG GCCCACAGGA 
CGGGGCACAG ATGAGCTGCT AGGTGTCATG GACCAGGTCA CCATCATCAA CTCCACCCTG 
GGGAAGGCGC TGGGTGGAGC ATCAGGGGGA TATACCACCG GGCCTGAGCC ACTGGTGTCC 
CTGCTACGGC AGCGTTCTCG GCCCTACCTC TTCTCCAACA GCCTGCCCCC TGCTGTAGTC 
GGCTGTGCTT CTAAGGCCCT GGACCTGCTC ATGGAGAGCA ATGCCATCAT CCAGTCTATG 
GCTGCCAAGA CCCGGCGGTT CCGCAGTAAG ATGGAAGCTG CTGGTTTTAC TGTCTCAGGA 
GCCGATCACC CCATCTGCCC TGTGATGCTG GGAGATGCAC GGTTGTCTTC TCAAATGGCA 
GATGACATGT TAAAGAAAGG AAAATGGCTG ACATCCAGAA CATTTCGCTG GACAGCCCAG 
GGAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gcat ( NM_013847.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038875.3 Mus musculus glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) (Gcat), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16202
Clone ID Related Accession (Same CDS sequence)	NM_013847.4
Accession Version	NM_013847.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL589670.21, AK087433.1, AK075782.1 and AK013138.1. On Jun 18, 2009 this sequence version replaced NM_013847.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC024107.1, AK013138.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTGGGCCA GCTTCATGTG GCACGGGGCG CTGTCCCCTG GCCGCCGCGC ACATTCAGCG 
CTGGCTCAGT TGCGCTGCAT CCTGGACAGC GAACTGGAAG GGATCCGCGG AGCCGGCACC 
TGGAAGAGTG AGCGTGTGAT CACGTCCCGC CAGGGACCGA GCATCCGCGT GGACGGCATC 
TCGGGAGGAA TCCTCAACTT CTGTGCCAAT AACTACCTGG GCCTGAGCAG CCACCCTGCA 
GTGATCCAGG CAGGTCTGCA GACTCTGGAG GAGTTTGGAG CTGGACTCAG TTCTACTCGA 
TTTATCTGTG GGACCCAGAG CATCCATAAG AATCTAGAAG CCAAGATAGC CCACTTCCAC 
CAGCGTGAGG ACGCCATCCT CTATCCCAGC TGTTTCGATG CCAACGCTGG CCTATTTGAG 
GCTTTGCTGA CCCCAGAGGA TGCGGTTCTG TCAGATGAGC TGAACCATGC TTCCATCATT 
GACGGCATCC GACTGTGCAA GGCCCACAAG TACCGTTACC GCCACCTGGA CATGGCTGAC 
CTGGAAGCCA AGCTGAAGGA GGCTCAGAAG CACAGGCTGC GCCTGGTGGC CACAGATGGA 
GCTTTCTCCA TGGATGGTGA CATCGCTCCC CTACAGGACA TCTGCCGCCT TGCCGCCCAA 
TATGGTGCCC TGGTCTTTGT GGATGAATGC CATGCCACTG GCTTTCTTGG GCCCACAGGA 
CGGGGCACAG ATGAGCTGCT AGGTGTCATG GACCAGGTCA CCATCATCAA CTCCACCCTG 
GGGAAGGCGC TGGGTGGAGC ATCAGGGGGA TATACCACCG GGCCTGAGCC ACTGGTGTCC 
CTGCTACGGC AGCGTTCTCG GCCCTACCTC TTCTCCAACA GCCTGCCCCC TGCTGTAGTC 
GGCTGTGCTT CTAAGGCCCT GGACCTGCTC ATGGAGAGCA ATGCCATCAT CCAGTCTATG 
GCTGCCAAGA CCCGGCGGTT CCGCAGTAAG ATGGAAGCTG CTGGTTTTAC TGTCTCAGGA 
GCCGATCACC CCATCTGCCC TGTGATGCTG GGAGATGCAC GGTTGTCTTC TCAAATGGCA 
GATGACATGT TAAAGAAAGG TATCTTTGTC ATCGGATTTA GCTACCCTGT GGTCCCTAAG 
GGCAAGGCCC GGATCCGGGT GCAGATCTCA GCTGTACACA GTGAGGAAGA CATTGACCGC 
TGTGTGGAAG CCTTCGTGGA GGTGGGGCGG CTGCATGGAG CTCTGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038875.3
CDS	61..1311
Translation	MWASFMWHGALSPGRRAHSALAQLRCILDSELEGIRGAGTWKSERVITSRQGPSIRVDGISGGILNFCANNYLGLSSHPAVIQAGLQTLEEFGAGLSSTRFICGTQSIHKNLEAKIAHFHQREDAILYPSCFDANAGLFEALLTPEDAVLSDELNHASIIDGIRLCKAHKYRYRHLDMADLEAKLKEAQKHRLRLVATDGAFSMDGDIAPLQDICRLAAQYGALVFVDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTTGPEPLVSLLRQRSRPYLFSNSLPPAVVGCASKALDLLMESNAIIQSMAAKTRRFRSKMEAAGFTVSGADHPICPVMLGDARLSSQMADDMLKKGIFVIGFSYPVVPKGKARIRVQISAVHSEEDIDRCVEAFVEVGRLHGALP

Target ORF information:

RefSeq Version	NM_013847.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) (Gcat), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013847.4

ORF Insert Sequence:

ATGTGGGCCA GCTTCATGTG GCACGGGGCG CTGTCCCCTG GCCGCCGCGC ACATTCAGCG 
CTGGCTCAGT TGCGCTGCAT CCTGGACAGC GAACTGGAAG GGATCCGCGG AGCCGGCACC 
TGGAAGAGTG AGCGTGTGAT CACGTCCCGC CAGGGACCGA GCATCCGCGT GGACGGCATC 
TCGGGAGGAA TCCTCAACTT CTGTGCCAAT AACTACCTGG GCCTGAGCAG CCACCCTGCA 
GTGATCCAGG CAGGTCTGCA GACTCTGGAG GAGTTTGGAG CTGGACTCAG TTCTACTCGA 
TTTATCTGTG GGACCCAGAG CATCCATAAG AATCTAGAAG CCAAGATAGC CCACTTCCAC 
CAGCGTGAGG ACGCCATCCT CTATCCCAGC TGTTTCGATG CCAACGCTGG CCTATTTGAG 
GCTTTGCTGA CCCCAGAGGA TGCGGTTCTG TCAGATGAGC TGAACCATGC TTCCATCATT 
GACGGCATCC GACTGTGCAA GGCCCACAAG TACCGTTACC GCCACCTGGA CATGGCTGAC 
CTGGAAGCCA AGCTGAAGGA GGCTCAGAAG CACAGGCTGC GCCTGGTGGC CACAGATGGA 
GCTTTCTCCA TGGATGGTGA CATCGCTCCC CTACAGGACA TCTGCCGCCT TGCCGCCCAA 
TATGGTGCCC TGGTCTTTGT GGATGAATGC CATGCCACTG GCTTTCTTGG GCCCACAGGA 
CGGGGCACAG ATGAGCTGCT AGGTGTCATG GACCAGGTCA CCATCATCAA CTCCACCCTG 
GGGAAGGCGC TGGGTGGAGC ATCAGGGGGA TATACCACCG GGCCTGAGCC ACTGGTGTCC 
CTGCTACGGC AGCGTTCTCG GCCCTACCTC TTCTCCAACA GCCTGCCCCC TGCTGTAGTC 
GGCTGTGCTT CTAAGGCCCT GGACCTGCTC ATGGAGAGCA ATGCCATCAT CCAGTCTATG 
GCTGCCAAGA CCCGGCGGTT CCGCAGTAAG ATGGAAGCTG CTGGTTTTAC TGTCTCAGGA 
GCCGATCACC CCATCTGCCC TGTGATGCTG GGAGATGCAC GGTTGTCTTC TCAAATGGCA 
GATGACATGT TAAAGAAAGG TATCTTTGTC ATCGGATTTA GCTACCCTGT GGTCCCTAAG 
GGCAAGGCCC GGATCCGGGT GCAGATCTCA GCTGTACACA GTGAGGAAGA CATTGACCGC 
TGTGTGGAAG CCTTCGTGGA GGTGGGGCGG CTGCATGGAG CTCTGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mice deficient in the Shmt2 gene have mitochondrial respiration defects and are embryonic lethal.
Scientific reports8(1)425(2018 Jan)
Tani H,Ohnishi S,Shitara H,Mito T,Yamaguchi M,Yonekawa H,Hashizume O,Ishikawa K,Nakada K,Hayashi JI

Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase by cold and osmotic stress.
Cell stress & chaperones12(2)186-91(2007)
Hoshino A,Fujii H

Proteomic analysis of the mouse liver mitochondrial inner membrane.
The Journal of biological chemistry278(42)41566-71(2003 Oct)
Da Cruz S,Xenarios I,Langridge J,Vilbois F,Parone PA,Martinou JC

Molecular cloning of the human and murine 2-amino-3-ketobutyrate coenzyme A ligase cDNAs.
European journal of biochemistry267(6)1805-12(2000 Mar)
Edgar AJ,Polak JM