Clcn6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clcn6
Entrez Gene ID	26372
Full Name	chloride channel, voltage-sensitive 6
Synonyms	AI850629,Clc6
General protein information	Preferred Names chloride channel, voltage-sensitive 6 Names chloride transport protein 6 chloride channel 6 chloride channel protein 6 clC-6
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 E2\|4 78.67 cM
Summary	This gene encodes a member of the ClC chloride channel and transporter family of proteins. The encoded protein may function as a vesicular Cl-/H+ antiporter. Homozygous knockout mice exhibit decreased pain sensitivity, behavioral abnormalities and features of lysosomal storage disease. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006538876.1	XP_006538939.1	chloride transport protein 6 isoform X1
NM_011929.3	NP_036059.1	chloride transport protein 6

Pathways from BioSystems

Homologies

Homo sapiens (human)	CLCN6	NP_001277.1
Pan troglodytes (chimpanzee)	CLCN6	XP_001140805.1
Mus musculus (house mouse)	Clcn6	NP_036059.1
Danio rerio (zebrafish)	clcn6	XP_696527.3
Rattus norvegicus (Norway rat)	Clcn6	NP_001099949.1
	CLCN6	XP_001104721.1
Bos taurus (cattle)	CLCN6	NP_001192799.1
Gallus gallus (chicken)	CLCN6	XP_417644.2
Xenopus tropicalis (tropical clawed frog)	clcn6	XP_002937575.2
Canis lupus familiaris (dog)	CLCN6	XP_005618051.1
Arabidopsis thaliana (thale cress)	CLC-D	NP_197996.1

Gene Ontology
Bibliography

Related articles in PubMed

Ion channel expression in the developing enteric nervous system.
Hirst CS, Foong JP, Stamp LA, Fegan E, Dent S, Cooper EC, Lomax AE, Anderson CR, Bornstein JC, Young HM, McKeown SJ
PloS one10(3)e0123436(2015)

Changing expression of chloride channels during preimplantation mouse development.
Arnaiz I, Johnson MH, Cook DI, Day ML
Reproduction (Cambridge, England)145(1)73-84(2013 Jan)

Patterned expression of ion channel genes in mouse dorsal raphe nucleus determined with the Allen Mouse Brain Atlas.
Templin JS, Bang SJ, Soiza-Reilly M, Berde CB, Commons KG
Brain research14571-12(2012 May)

Distinct neuropathologic phenotypes after disrupting the chloride transport proteins ClC-6 or ClC-7/Ostm1.
Pressey SN, O'Donnell KJ, Stauber T, Fuhrmann JC, Tyynel? J, Jentsch TJ, Cooper JD
Journal of neuropathology and experimental neurology69(12)1228-46(2010 Dec)

Chloride and the endosomal-lysosomal pathway: emerging roles of CLC chloride transporters.
Jentsch TJ
The Journal of physiology578(Pt 3)633-40(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonistrated that clcn6 gene expression in mouse dorsal raphe nucleus
Title: Patterned expression of ion channel genes in mouse dorsal raphe nucleus determined with the Allen Mouse Brain Atlas.

Chloride channel 6 (Clcn6) deficient mice have a later-onset form of mild neurologic dysfunction similar to neuronal ceroid lipofuscinosis.
Title: Distinct neuropathologic phenotypes after disrupting the chloride transport proteins ClC-6 or ClC-7/Ostm1.

ClC-6 protein is almost exclusively expressed in neurons of the central and peripheral nervous systems, with a particularly high expression in dorsal root ganglia.
Title: Lysosomal storage disease upon disruption of the neuronal chloride transport protein ClC-6.

The following Clcn6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clcn6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu34207	XM_006538876.1 Latest version!	Mus musculus chloride channel, voltage-sensitive 6 (Clcn6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu09178	NM_011929.3 Latest version!	Mus musculus chloride channel, voltage-sensitive 6 (Clcn6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clcn6 ( XM_006538876.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538939.1 Mus musculus chloride channel, voltage-sensitive 6 (Clcn6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu34207
Clone ID Related Accession (Same CDS sequence)	XM_006538876.1
Accession Version	XM_006538876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chloride transport protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTAG GAGAATGCCG ACAGATGTCT TCCACGAGTC AAACCGGTAA TGGCTCATTC 
CAGCTCCAGG TCACATCAGA AGATGTGAAT TCAACTATCA AGGCTTTTTT CTGTCCCAAT 
GACACTTACA ATGACATGGC CACACTCTTC TTCAACTCCC AGGAGTCTGC CATCCTGCAG 
CTCTTCCATC AGGATGGGAC TTTCAGCCCC GTCACCCTGG CCTTATTCTT CATCCTCTAT 
TTCCTGCTCG CATGCTGGAC TTTTGGCACA TCTGTCCCCA GTGGCCTCTT CGTGCCTTCC 
CTGCTGTGTG GAGCAGCTTT TGGACGTTTA GTTGCCAATG TCCTGAAAAG CTACATTGGA 
CTGGGCCACC TCTATTCTGG GACCTTTGCC CTGATTGGTG CGGCAGCTTT CTTGGGTGGG 
GTTGTAAGGA TGACCATCAG CCTCACAGTC ATCCTGATCG AGTCTACAAA TGAGATCACC 
TATGGGCTTC CGATCATGGT CACTCTGATG GTGGCCAAGT GGACTGGAGA CTTGTTCAAC 
AAGGGCATTT ATGACGTCCA TATTGGGCTG AGAGGTGTGC CTCTTCTGGA GTGGGAGACA 
GACGTGGAAA TGGACAAGCT GAGAGCCAGT GACATCATGG AGCCCAACCT GACGTACGTC 
TACCCGCACA CTCGCATCCA GTCTCTGGTG AGCATCCTGC GCACTACGGT CCACCATGCC 
TTCCCAGTGG TCACGGAGAA CCGGGGCAAC GAGAAGGAGT TCATGAAGGG CAACCAGCTC 
ATAAGCAACA ACATCAAGTT CAAGAAATCC AGTATCCTGA CCCGTGCTGG TGAGCAGCGC 
AAGCGGGGCC AGTCCATGAA GTCGTACCCT TCCAGCGAGC TGCGCAACGT GTGTGATGAG 
CATGTGGCTT CTGAGGAGCC GGCAGAGAAG GAGGACCTGC TACAGCAGAT GCTGGAGAGG 
AGATACACTC CCTACCCCAA CCTATACCCT GACCAGTCCC CGAGTGAAGA CTGGACCATG 
GAGGAGCGTT TCCGTCCGCT GACCTTTCAC GGCCTTGTCC TGCGCTCTCA GCTTGTCACC 
CTGCTTGTCC GAGGAGTGTG CTATTCGGAA AGTCAGTCCA GTGCCAGCCA ACCACGTCTT 
TCTTACGCTG AGATGGCTGA GGACTACCCG CGATACCCGG ACATACATGA CCTGGACCTC 
ACACTGCTGA ATCCCCGAAT GATTGTGGAT GTCACCCCAT ATATGAATCC TTCACCATTT 
ACCGTCTCCC CCAACACCCA TGTTTCTCAA GTGTTCAACC TGTTCAGAAC CATGGGCCTG 
CGCCACTTGC CTGTGGTGAA TGCAGTAGGA GAGATCGTGG GGATCATTAC ACGGCACAAC 
CTGACAAATG AATTCCTGCA GGCCCGGCTG CGGCAGCATT ACCAGACCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538939.1
CDS	667..2100
Translation	MVLGECRQMSSTSQTGNGSFQLQVTSEDVNSTIKAFFCPNDTYNDMATLFFNSQESAILQLFHQDGTFSPVTLALFFILYFLLACWTFGTSVPSGLFVPSLLCGAAFGRLVANVLKSYIGLGHLYSGTFALIGAAAFLGGVVRMTISLTVILIESTNEITYGLPIMVTLMVAKWTGDLFNKGIYDVHIGLRGVPLLEWETDVEMDKLRASDIMEPNLTYVYPHTRIQSLVSILRTTVHHAFPVVTENRGNEKEFMKGNQLISNNIKFKKSSILTRAGEQRKRGQSMKSYPSSELRNVCDEHVASEEPAEKEDLLQQMLERRYTPYPNLYPDQSPSEDWTMEERFRPLTFHGLVLRSQLVTLLVRGVCYSESQSSASQPRLSYAEMAEDYPRYPDIHDLDLTLLNPRMIVDVTPYMNPSPFTVSPNTHVSQVFNLFRTMGLRHLPVVNAVGEIVGIITRHNLTNEFLQARLRQHYQTL

Target ORF information:

RefSeq Version	XM_006538876.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 6 (Clcn6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538876.1

ORF Insert Sequence:

ATGGTGTTAG GAGAATGCCG ACAGATGTCT TCCACGAGTC AAACCGGTAA TGGCTCATTC 
CAGCTCCAGG TCACATCAGA AGATGTGAAT TCAACTATCA AGGCTTTTTT CTGTCCCAAT 
GACACTTACA ATGACATGGC CACACTCTTC TTCAACTCCC AGGAGTCTGC CATCCTGCAG 
CTCTTCCATC AGGATGGGAC TTTCAGCCCC GTCACCCTGG CCTTATTCTT CATCCTCTAT 
TTCCTGCTCG CATGCTGGAC TTTTGGCACA TCTGTCCCCA GTGGCCTCTT CGTGCCTTCC 
CTGCTGTGTG GAGCAGCTTT TGGACGTTTA GTTGCCAATG TCCTGAAAAG CTACATTGGA 
CTGGGCCACC TCTATTCTGG GACCTTTGCC CTGATTGGTG CGGCAGCTTT CTTGGGTGGG 
GTTGTAAGGA TGACCATCAG CCTCACAGTC ATCCTGATCG AGTCTACAAA TGAGATCACC 
TATGGGCTTC CGATCATGGT CACTCTGATG GTGGCCAAGT GGACTGGAGA CTTGTTCAAC 
AAGGGCATTT ATGACGTCCA TATTGGGCTG AGAGGTGTGC CTCTTCTGGA GTGGGAGACA 
GACGTGGAAA TGGACAAGCT GAGAGCCAGT GACATCATGG AGCCCAACCT GACGTACGTC 
TACCCGCACA CTCGCATCCA GTCTCTGGTG AGCATCCTGC GCACTACGGT CCACCATGCC 
TTCCCAGTGG TCACGGAGAA CCGGGGCAAC GAGAAGGAGT TCATGAAGGG CAACCAGCTC 
ATAAGCAACA ACATCAAGTT CAAGAAATCC AGTATCCTGA CCCGTGCTGG TGAGCAGCGC 
AAGCGGGGCC AGTCCATGAA GTCGTACCCT TCCAGCGAGC TGCGCAACGT GTGTGATGAG 
CATGTGGCTT CTGAGGAGCC GGCAGAGAAG GAGGACCTGC TACAGCAGAT GCTGGAGAGG 
AGATACACTC CCTACCCCAA CCTATACCCT GACCAGTCCC CGAGTGAAGA CTGGACCATG 
GAGGAGCGTT TCCGTCCGCT GACCTTTCAC GGCCTTGTCC TGCGCTCTCA GCTTGTCACC 
CTGCTTGTCC GAGGAGTGTG CTATTCGGAA AGTCAGTCCA GTGCCAGCCA ACCACGTCTT 
TCTTACGCTG AGATGGCTGA GGACTACCCG CGATACCCGG ACATACATGA CCTGGACCTC 
ACACTGCTGA ATCCCCGAAT GATTGTGGAT GTCACCCCAT ATATGAATCC TTCACCATTT 
ACCGTCTCCC CCAACACCCA TGTTTCTCAA GTGTTCAACC TGTTCAGAAC CATGGGCCTG 
CGCCACTTGC CTGTGGTGAA TGCAGTAGGA GAGATCGTGG GGATCATTAC ACGGCACAAC 
CTGACAAATG AATTCCTGCA GGCCCGGCTG CGGCAGCATT ACCAGACCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcn6 ( NM_011929.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036059.1 Mus musculus chloride channel, voltage-sensitive 6 (Clcn6), mRNA.

Price & Availability↑

CloneID	OMu09178
Clone ID Related Accession (Same CDS sequence)	NM_011929.3
Accession Version	NM_011929.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	chloride transport protein 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK165362.1, CX564347.1, CB521277.1, AL606929.13, CX205353.1, BQ178271.1, BM949055.1 and BQ174789.1. On Aug 22, 2015 this sequence version replaced NM_011929.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK165362.1, SRR1660813.17519.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT GCAGGGGGTC CGTGTGCTGC TGCTGCCGGT GGTGTTGCTG CTGCGGTGAA 
CGGGAGAGCC GCACCCCCGA GGAGCTGACC ATCCTTGGGG AGACACAGGA AGAAGAGGAT 
GAGATTCTTC CAAGGAAAGA CTATGAGAGT CTGGACTATG ACCGCTGCAT CAATGACCCT 
TACCTGGAGG TGTTGGAGAC CATGGATAAT AAGAAAGGGC GAAGATACGA GGCGGTGAAG 
TGGATGGTGG TGTTCGCCAT TGGCGTCTGT ACGGGGCTGG TGGGTCTCTT TGTGGACTTT 
TCCGTGCGAC TCTTCACCCA ACTGAAGTTC GGAGTGGTTC AAACGTCGGT GGAGGAATGC 
AGTCAGAAGG GCTGCCTTGC CCTGTCCCTC CTGGAGCTCC TGGGCTTTAA CTTGACCTTT 
GTCTTCCTGG CAAGCCTTCT TGTCTTGATA GAGCCAGTGG CAGCAGGTTC TGGAATCCCC 
GAGATCAAGT GCTACCTGAA TGGTGTGAAA GTGCCAGGAA TTGTCCGGCT CCGGACCCTG 
CTCTGCAAAG TCTTTGGGGT GCTGTTCAGT GTGTCTGGAG GGCTCTTTGT GGGGAAAGAA 
GGCCCCATGA TCCACAGTGG TGCTGTGGTA GGAGCTGGCC TCCCTCAGTT CCAGAGCATC 
TCCTTACGGA AGATCCAGTT TAACTTCCCC TACTTCCGCA GCGACAGAGA CAAGCGTGAC 
TTTGTGTCAG CGGGGGCAGC TGCTGGCGTT GCTGCAGCTT TTGGGGCCCC TATAGGGGGC 
ACCTTGTTCA GTCTGGAGGA GGGTTCATCC TTCTGGAACC AGGGGCTCAC GTGGAAAGTG 
CTCTTCTGTT CCATGTCCGC CACCTTCACC CTCAACTTCT TCCGCTCTGG GATTCAGTTT 
GGAAGTTGGG GTTCCTTCCA GCTGCCTGGA TTGCTGAACT TTGGTGAATT TAAGTGCTCT 
GACTCTGATA AAAAATGTCA TCTCTGGACA GCTATGGATT TGGGTTTCTT CGTCGTGATG 
GGGGTCATTG GGGGCCTCCT GGGAGCCACA TTCAACTGTC TGAACAAGAG GCTTGCAAAG 
TACCGTATGC GCAACGTGCA CCCGAAACCT AAGCTCGTCA GAGTCTTAGA GAGCCTCTTG 
GTGTCCCTGG TGACCACTGT GGTGGTGTTT GTGGCCTCCA TGGTGTTAGG AGAATGCCGA 
CAGATGTCTT CCACGAGTCA AACCGGTAAT GGCTCATTCC AGCTCCAGGT CACATCAGAA 
GATGTGAATT CAACTATCAA GGCTTTTTTC TGTCCCAATG ACACTTACAA TGACATGGCC 
ACACTCTTCT TCAACTCCCA GGAGTCTGCC ATCCTGCAGC TCTTCCATCA GGATGGGACT 
TTCAGCCCCG TCACCCTGGC CTTATTCTTC ATCCTCTATT TCCTGCTCGC ATGCTGGACT 
TTTGGCACAT CTGTCCCCAG TGGCCTCTTC GTGCCTTCCC TGCTGTGTGG AGCAGCTTTT 
GGACGTTTAG TTGCCAATGT CCTGAAAAGC TACATTGGAC TGGGCCACCT CTATTCTGGG 
ACCTTTGCCC TGATTGGTGC GGCAGCTTTC TTGGGTGGGG TTGTAAGGAT GACCATCAGC 
CTCACAGTCA TCCTGATCGA GTCTACAAAT GAGATCACCT ATGGGCTTCC GATCATGGTC 
ACTCTGATGG TGGCCAAGTG GACTGGAGAC TTGTTCAACA AGGGCATTTA TGACGTCCAT 
ATTGGGCTGA GAGGTGTGCC TCTTCTGGAG TGGGAGACAG ACGTGGAAAT GGACAAGCTG 
AGAGCCAGTG ACATCATGGA GCCCAACCTG ACGTACGTCT ACCCGCACAC TCGCATCCAG 
TCTCTGGTGA GCATCCTGCG CACTACGGTC CACCATGCCT TCCCAGTGGT CACGGAGAAC 
CGGGGCAACG AGAAGGAGTT CATGAAGGGC AACCAGCTCA TAAGCAACAA CATCAAGTTC 
AAGAAATCCA GTATCCTGAC CCGTGCTGGT GAGCAGCGCA AGCGGGGCCA GTCCATGAAG 
TCGTACCCTT CCAGCGAGCT GCGCAACGTG TGTGATGAGC ATGTGGCTTC TGAGGAGCCG 
GCAGAGAAGG AGGACCTGCT ACAGCAGATG CTGGAGAGGA GATACACTCC CTACCCCAAC 
CTATACCCTG ACCAGTCCCC GAGTGAAGAC TGGACCATGG AGGAGCGTTT CCGTCCGCTG 
ACCTTTCACG GCCTTGTCCT GCGCTCTCAG CTTGTCACCC TGCTTGTCCG AGGAGTGTGC 
TATTCGGAAA GTCAGTCCAG TGCCAGCCAA CCACGTCTTT CTTACGCTGA GATGGCTGAG 
GACTACCCGC GATACCCGGA CATACATGAC CTGGACCTCA CACTGCTGAA TCCCCGAATG 
ATTGTGGATG TCACCCCATA TATGAATCCT TCACCATTTA CCGTCTCCCC CAACACCCAT 
GTTTCTCAAG TGTTCAACCT GTTCAGAACC ATGGGCCTGC GCCACTTGCC TGTGGTGAAT 
GCAGTAGGAG AGATCGTGGG GATCATTACA CGGCACAACC TGACAAATGA ATTCCTGCAG 
GCCCGGCTGC GGCAGCATTA CCAGACCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036059.1
CDS	47..2659
Translation	MAGCRGSVCCCCRWCCCCGERESRTPEELTILGETQEEEDEILPRKDYESLDYDRCINDPYLEVLETMDNKKGRRYEAVKWMVVFAIGVCTGLVGLFVDFSVRLFTQLKFGVVQTSVEECSQKGCLALSLLELLGFNLTFVFLASLLVLIEPVAAGSGIPEIKCYLNGVKVPGIVRLRTLLCKVFGVLFSVSGGLFVGKEGPMIHSGAVVGAGLPQFQSISLRKIQFNFPYFRSDRDKRDFVSAGAAAGVAAAFGAPIGGTLFSLEEGSSFWNQGLTWKVLFCSMSATFTLNFFRSGIQFGSWGSFQLPGLLNFGEFKCSDSDKKCHLWTAMDLGFFVVMGVIGGLLGATFNCLNKRLAKYRMRNVHPKPKLVRVLESLLVSLVTTVVVFVASMVLGECRQMSSTSQTGNGSFQLQVTSEDVNSTIKAFFCPNDTYNDMATLFFNSQESAILQLFHQDGTFSPVTLALFFILYFLLACWTFGTSVPSGLFVPSLLCGAAFGRLVANVLKSYIGLGHLYSGTFALIGAAAFLGGVVRMTISLTVILIESTNEITYGLPIMVTLMVAKWTGDLFNKGIYDVHIGLRGVPLLEWETDVEMDKLRASDIMEPNLTYVYPHTRIQSLVSILRTTVHHAFPVVTENRGNEKEFMKGNQLISNNIKFKKSSILTRAGEQRKRGQSMKSYPSSELRNVCDEHVASEEPAEKEDLLQQMLERRYTPYPNLYPDQSPSEDWTMEERFRPLTFHGLVLRSQLVTLLVRGVCYSESQSSASQPRLSYAEMAEDYPRYPDIHDLDLTLLNPRMIVDVTPYMNPSPFTVSPNTHVSQVFNLFRTMGLRHLPVVNAVGEIVGIITRHNLTNEFLQARLRQHYQTL

Target ORF information:

RefSeq Version	NM_011929.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 6 (Clcn6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011929.3

ORF Insert Sequence:

ATGGCGGGGT GCAGGGGGTC CGTGTGCTGC TGCTGCCGGT GGTGTTGCTG CTGCGGTGAA 
CGGGAGAGCC GCACCCCCGA GGAGCTGACC ATCCTTGGGG AGACACAGGA AGAAGAGGAT 
GAGATTCTTC CAAGGAAAGA CTATGAGAGT CTGGACTATG ACCGCTGCAT CAATGACCCT 
TACCTGGAGG TGTTGGAGAC CATGGATAAT AAGAAAGGGC GAAGATACGA GGCGGTGAAG 
TGGATGGTGG TGTTCGCCAT TGGCGTCTGT ACGGGGCTGG TGGGTCTCTT TGTGGACTTT 
TCCGTGCGAC TCTTCACCCA ACTGAAGTTC GGAGTGGTTC AAACGTCGGT GGAGGAATGC 
AGTCAGAAGG GCTGCCTTGC CCTGTCCCTC CTGGAGCTCC TGGGCTTTAA CTTGACCTTT 
GTCTTCCTGG CAAGCCTTCT TGTCTTGATA GAGCCAGTGG CAGCAGGTTC TGGAATCCCC 
GAGATCAAGT GCTACCTGAA TGGTGTGAAA GTGCCAGGAA TTGTCCGGCT CCGGACCCTG 
CTCTGCAAAG TCTTTGGGGT GCTGTTCAGT GTGTCTGGAG GGCTCTTTGT GGGGAAAGAA 
GGCCCCATGA TCCACAGTGG TGCTGTGGTA GGAGCTGGCC TCCCTCAGTT CCAGAGCATC 
TCCTTACGGA AGATCCAGTT TAACTTCCCC TACTTCCGCA GCGACAGAGA CAAGCGTGAC 
TTTGTGTCAG CGGGGGCAGC TGCTGGCGTT GCTGCAGCTT TTGGGGCCCC TATAGGGGGC 
ACCTTGTTCA GTCTGGAGGA GGGTTCATCC TTCTGGAACC AGGGGCTCAC GTGGAAAGTG 
CTCTTCTGTT CCATGTCCGC CACCTTCACC CTCAACTTCT TCCGCTCTGG GATTCAGTTT 
GGAAGTTGGG GTTCCTTCCA GCTGCCTGGA TTGCTGAACT TTGGTGAATT TAAGTGCTCT 
GACTCTGATA AAAAATGTCA TCTCTGGACA GCTATGGATT TGGGTTTCTT CGTCGTGATG 
GGGGTCATTG GGGGCCTCCT GGGAGCCACA TTCAACTGTC TGAACAAGAG GCTTGCAAAG 
TACCGTATGC GCAACGTGCA CCCGAAACCT AAGCTCGTCA GAGTCTTAGA GAGCCTCTTG 
GTGTCCCTGG TGACCACTGT GGTGGTGTTT GTGGCCTCCA TGGTGTTAGG AGAATGCCGA 
CAGATGTCTT CCACGAGTCA AACCGGTAAT GGCTCATTCC AGCTCCAGGT CACATCAGAA 
GATGTGAATT CAACTATCAA GGCTTTTTTC TGTCCCAATG ACACTTACAA TGACATGGCC 
ACACTCTTCT TCAACTCCCA GGAGTCTGCC ATCCTGCAGC TCTTCCATCA GGATGGGACT 
TTCAGCCCCG TCACCCTGGC CTTATTCTTC ATCCTCTATT TCCTGCTCGC ATGCTGGACT 
TTTGGCACAT CTGTCCCCAG TGGCCTCTTC GTGCCTTCCC TGCTGTGTGG AGCAGCTTTT 
GGACGTTTAG TTGCCAATGT CCTGAAAAGC TACATTGGAC TGGGCCACCT CTATTCTGGG 
ACCTTTGCCC TGATTGGTGC GGCAGCTTTC TTGGGTGGGG TTGTAAGGAT GACCATCAGC 
CTCACAGTCA TCCTGATCGA GTCTACAAAT GAGATCACCT ATGGGCTTCC GATCATGGTC 
ACTCTGATGG TGGCCAAGTG GACTGGAGAC TTGTTCAACA AGGGCATTTA TGACGTCCAT 
ATTGGGCTGA GAGGTGTGCC TCTTCTGGAG TGGGAGACAG ACGTGGAAAT GGACAAGCTG 
AGAGCCAGTG ACATCATGGA GCCCAACCTG ACGTACGTCT ACCCGCACAC TCGCATCCAG 
TCTCTGGTGA GCATCCTGCG CACTACGGTC CACCATGCCT TCCCAGTGGT CACGGAGAAC 
CGGGGCAACG AGAAGGAGTT CATGAAGGGC AACCAGCTCA TAAGCAACAA CATCAAGTTC 
AAGAAATCCA GTATCCTGAC CCGTGCTGGT GAGCAGCGCA AGCGGGGCCA GTCCATGAAG 
TCGTACCCTT CCAGCGAGCT GCGCAACGTG TGTGATGAGC ATGTGGCTTC TGAGGAGCCG 
GCAGAGAAGG AGGACCTGCT ACAGCAGATG CTGGAGAGGA GATACACTCC CTACCCCAAC 
CTATACCCTG ACCAGTCCCC GAGTGAAGAC TGGACCATGG AGGAGCGTTT CCGTCCGCTG 
ACCTTTCACG GCCTTGTCCT GCGCTCTCAG CTTGTCACCC TGCTTGTCCG AGGAGTGTGC 
TATTCGGAAA GTCAGTCCAG TGCCAGCCAA CCACGTCTTT CTTACGCTGA GATGGCTGAG 
GACTACCCGC GATACCCGGA CATACATGAC CTGGACCTCA CACTGCTGAA TCCCCGAATG 
ATTGTGGATG TCACCCCATA TATGAATCCT TCACCATTTA CCGTCTCCCC CAACACCCAT 
GTTTCTCAAG TGTTCAACCT GTTCAGAACC ATGGGCCTGC GCCACTTGCC TGTGGTGAAT 
GCAGTAGGAG AGATCGTGGG GATCATTACA CGGCACAACC TGACAAATGA ATTCCTGCAG 
GCCCGGCTGC GGCAGCATTA CCAGACCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ion channel expression in the developing enteric nervous system.
PloS one10(3)e0123436(2015)
Hirst CS,Foong JP,Stamp LA,Fegan E,Dent S,Cooper EC,Lomax AE,Anderson CR,Bornstein JC,Young HM,McKeown SJ

Changing expression of chloride channels during preimplantation mouse development.
Reproduction (Cambridge, England)145(1)73-84(2013 Jan)
Arnaiz I,Johnson MH,Cook DI,Day ML

Patterned expression of ion channel genes in mouse dorsal raphe nucleus determined with the Allen Mouse Brain Atlas.
Brain research14571-12(2012 May)
Templin JS,Bang SJ,Soiza-Reilly M,Berde CB,Commons KG

Distinct neuropathologic phenotypes after disrupting the chloride transport proteins ClC-6 or ClC-7/Ostm1.
Journal of neuropathology and experimental neurology69(12)1228-46(2010 Dec)
Pressey SN,O'Donnell KJ,Stauber T,Fuhrmann JC,Tyynel? J,Jentsch TJ,Cooper JD

Chloride and the endosomal-lysosomal pathway: emerging roles of CLC chloride transporters.
The Journal of physiology578(Pt 3)633-40(2007 Feb)
Jentsch TJ