Gucy2f cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gucy2f
Entrez Gene ID	245650
Full Name	guanylate cyclase 2f
Synonyms	A930002I06,GC-F,GC2
General protein information	Preferred Names guanylate cyclase 2f Names retinal guanylyl cyclase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X F2\|X 62.85 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006528841.3	XP_006528904.1	retinal guanylyl cyclase 2 isoform X1
XM_006528841.2	XP_006528904.1	retinal guanylyl cyclase 2 isoform X1
XM_006528841.4	XP_006528904.1	retinal guanylyl cyclase 2 isoform X1
NM_001007576.2	NP_001007577.1	retinal guanylyl cyclase 2 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GUCY2F	NP_776974.1
Gallus gallus (chicken)	GUCY2F	XP_417281.3
Xenopus tropicalis (tropical clawed frog)	gucy2f	XP_002931862.1
Homo sapiens (human)	GUCY2F	NP_001513.2
	GUCY2F	XP_001098846.1
Rattus norvegicus (Norway rat)	Gucy2f	NP_446283.1
Pan troglodytes (chimpanzee)	GUCY2F	XP_003317673.1
Mus musculus (house mouse)	Gucy2f	NP_001007577.1
Canis lupus familiaris (dog)	GUCY2F	XP_005641716.1
Danio rerio (zebrafish)	gc2	NP_001103165.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional Study and Mapping Sites for Interaction with the Target Enzyme in Retinal Degeneration 3 (RD3) Protein.
Peshenko IV, Olshevskaya EV, Dizhoor AM
The Journal of biological chemistry291(37)19713-23(2016 09)

Retinal guanylyl cyclase isozyme 1 is the preferential in vivo target for constitutively active GCAP1 mutants causing congenital degeneration of photoreceptors.
Olshevskaya EV, Peshenko IV, Savchenko AB, Dizhoor AM
The Journal of neuroscience : the official journal of the Society for Neuroscience32(21)7208-17(2012 May)

Enzymatic relay mechanism stimulates cyclic GMP synthesis in rod photoresponse: biochemical and physiological study in guanylyl cyclase activating protein 1 knockout mice.
Makino CL, Wen XH, Olshevskaya EV, Peshenko IV, Savchenko AB, Dizhoor AM
PloS one7(10)e47637(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

rd3 colocalizes and interacts with GC1 and GC2 in rod and cone photoreceptor cells of normal mice
Title: RD3, the protein associated with Leber congenital amaurosis type 12, is required for guanylate cyclase trafficking in photoreceptor cells.

guanylate cyclase 1 and guanylate cyclase 2 have roles in function of rod and cone photoreceptors
Title: The function of guanylate cyclase 1 and guanylate cyclase 2 in rod and cone photoreceptors.

The following Gucy2f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gucy2f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10777	NM_001007576.2 Latest version!	Mus musculus guanylate cyclase 2f (Gucy2f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu10777	XM_006528841.4 Latest version!	Mus musculus guanylate cyclase 2f (Gucy2f), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu10777	XM_006528841.2	Mus musculus guanylate cyclase 2f (Gucy2f), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu10777	XM_006528841.3	Mus musculus guanylate cyclase 2f (Gucy2f), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gucy2f ( NM_001007576.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001007577.1 Mus musculus guanylate cyclase 2f (Gucy2f), mRNA.

Price & Availability↑

CloneID	OMu10777
Clone ID Related Accession (Same CDS sequence)	XM_006528841.3 , XM_006528841.2 , XM_006528841.4 , NM_001007576.2
Accession Version	NM_001007576.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	retinal guanylyl cyclase 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK044234.1, AY651761.1 and AL671916.5. On Feb 26, 2011 this sequence version replaced NM_001007576.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY651761.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849381, SAMN00849383 [ECO:0000350] ##Evidence-Data-END##

ATGTTCCTCG GACCCTGGCC TTTTTCTCGC CTTCTGTCCT GGTTTGCCAT TTCCAGCAGA 
CTGTCAGGAC AGCATGGCCT CCCTTCCTCC AAGTTCCTGA GATGTTTGTG CCTCTTGGCC 
CTTTTGCCTC TCCTTCGGTG GGGGCAAGCA CTCCCCTACA AGATCGGGGT CATAGGTCCC 
TGGACATGTG ATCCGTTCTT TTCCAAGGCC CTGCCTGAGG TTGCTGCTGC TTTAGCAATT 
GAGCGAATCA GCCGGGACAA GACATTTGAC AGGAGTTACT CCTTCGAATA CGTGATTCTT 
AACGAAGACT GTCAGACTTC AAAGGCCCTC GCCAGTTTCA TTTCCCACCA GCAGATGGCC 
TCAGGATTTG TTGGGCCTGC CAACCCTGGC TTCTGCGAGG CAGCCTCACT CCTGGGAACC 
AGCTGGGACA AAGGGATTTT CTCTTGGGCT TGTGTGAATC ATGAATTAGA TAACAAACAT 
AGCTTCCCAA CCTTCTCTCG GACACTCCCT TCTCCGATAC GGGTGCTTGT AACCGTCATG 
AAGTATTTCC AGTGGGCACA TGCTGGGGTC ATTTCCTCAG ATGAAGACAT TTGGATGCAT 
ACAGCCAATC GAGTCTCAAG TGCTCTTCGG AGCCAGGGCC TACCTGTAGG GGTCGTCCTG 
ACCTCGGGAC GAGACAGCCA GAGCATTCAG AAAGCTCTCC AGCAGATTCG CCAGGCAGAC 
AGAATTCGCA TAATCATAAT GTGTATGCAT TCAGCCTTGA TTGGGGGAGA GACACAAACA 
CACTTCTTGG AATTGGCTCA TGATCTGAAA ATGACAGATG GGACTTACGT CTTTGTCCCC 
TATGATGTAC TGCTCTACAG TTTACCTTAT AAGCACAGCC CCTACCAGGT CCTGAGGAAC 
AATCCAAAGC TCCGGGAAGC CTATGATGCT GTGTTGACTA TTACAGTGGA GTCCCATGAA 
AAGACCTTCT ACGAAGCCTA TGCAGAAGCA GCAGCCCGCG GAGAAATTCC TGAGAAGCCT 
GACTCAAACC AAGTTTCACC GTTGTTTGGA ACCATCTACA ATTCAATTTA TTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAAAAAAT GGACGGGCTA GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CTATGGATTC AACCAGTTGA TAAAGACAGA CTCAAATGGA 
AATGGGATTT CTGAATATGT AATCCTGGAC ACCAATGGGA AAGAATGGGA ACTTCGTGGC 
ACCTACACTG TGGACATGGA AACTGAACTG CTAAGGTTCA GAGGGACCCC AATTCATTTC 
CCTGGCGGCA GACCTACTAG TGCAGATGCA AAATGCTGGT TTGCAGAAAG GAAGATCTGC 
CAAGGAGGTA TTGACCCTGC CTTGGCCATG ATGGTCTGCT TTGCTTTACT TATAGCCTTG 
CTGTCTATCA ATGGATTTGC TTACTTTATA AGGCGACGTA TAAATAAAAT CCAGTTGATT 
AAAGGACCCA ACAGAATCCT ACTGACTTTG GAAGATGTGA CATTTATTAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGCCG TGCCAGTGTA AGCTTCCAGA TCATCTCAGA AGTTCAAAGT 
GGGAGGTCCC CAAGGCTGTC CTTTTCTTCA GGTAGTCTAA CTCCAGCTAC ATATGAGAAC 
TCCAACATAG CAATCTATGA GGGTGACTGG GTATGGCTGA AAAAGTTCCC TCCTGGAGAT 
TTTGGAGACA TTAAGTCTAT CAAATCGAGT GCAAGTGATG TGTTTGAAAT GATGAAGGAC 
CTGCGGCATG AAAATGTCAA CCCTCTATTG GGTTTCTTCT ATGATTCGGG GATGTTTGCC 
ATCGTGTCAG AATTTTGTTC TCGAAGGAGC CTAGAAGACA TACTTACAAA TGATGATGTG 
AAACTTGATT GGATGTTCAA ATCATCACTA CTGCTTGACC TCATCAAGGG GATGAAATAC 
TTACACCACA GAGAATTTAT TCACGGGAGG CTAAAGTCTC GGAACTGTGT GGTAGATGGG 
CGTTTTGTCC TAAAAGTGAC AGATTATGGC TTTAATGACA TTTTAGAAAT GCTGAGACTC 
TCTGAAGAGG AACCTTCTGA AGAAGAGCTG CTGTGGACGG CCCCTGAACT GTTAAGAGCC 
CCTGGGGGCA TCAGGTTAGG TTCATTTGCA GGAGACGTCT ATAGCTTTGC CATCATCATG 
CAAGAAGTGA TGGTTCGGGG TGCTCCTTTC TGCATGATGG ATCTGCCTGC CAAAGAAATC 
ATAGACAGAC TTAAAATGCC TCCCCCTGTG TACAGACCCG TGGTTTCTCC TGAGTATGCC 
CCTGCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCGTCAGA ACAGCGACCA 
ACTTTTGATG AAATATTTAA CCAGTTTAAA ACTTTCAACA AAGGGAAAAA GACTAATATT 
ATTGATTCTA TGCTTCGGAT GTTGGAGCAG TATTCTAGCA ACTTGGAAGA TTTGATCCGG 
GAACGGACTG AAGAATTGGA AATTGAAAAA CAGAAAACGG AAAAGCTTCT AACACAGATG 
CTACCACTAT CAGTTGCTGA ATCCCTCAAG AAGGGCTGTA CAGTTGAACC TGAGGGATTT 
GACTTGGTCA CCTTGTACTT CAGTGACATT GTGGGCTTCA CCACCATCTC AGCCATGAGT 
GAGCCCATTG AAGTTGTGGA CCTTCTGAAT GACCTGTACA CACTCTTTGA TGCCATCATT 
GGCAGTCATG ATGTCTATAA AGTAGAGACC ATTGGAGATG CCTACATGGT GGCCTCAGGC 
CTTCCCAAGA GGAATGGCAG TAGGCATGCA GCTGAGATTG CAAACATGTC CCTGGATATC 
CTGAGTTCTG TGGGTACCTT TAAGATGCGA CACATGCCAG AGGTGCCAGT CCGAATTCGA 
ATAGGCCTGC ACTCGGGGCC TGTTGTTGCT GGAGTGGTGG GCCTCACCAT GCCCAGATAT 
TGCTTGTTTG GGGACACTGT GAACACAGCT TCTCGAATGG AATCAACAGG GCTACCATAC 
CGAATTCATG TCAGTCTCAG CACAGTAACA ATTCTTCAAA CTCTGAGTGA AGGCTATGAA 
GTAGAGCTTC GTGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAGGAGAC CTTTTGGCTT 
GTTGGAAAAA AGGGTTTCAC CAAACCTCTT CCTGTACCCC CACCAGTGGG CAAAGATGGG 
CAAGTGGGCC ATGGCTTGCA ACCAGCAGAG ATTGCAGCAT TCCAAAGGAG AAAAGCAGAG 
AGGCAGTTGG TGCGAAACAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007577.1
CDS	246..3572
Translation	MFLGPWPFSRLLSWFAISSRLSGQHGLPSSKFLRCLCLLALLPLLRWGQALPYKIGVIGPWTCDPFFSKALPEVAAALAIERISRDKTFDRSYSFEYVILNEDCQTSKALASFISHQQMASGFVGPANPGFCEAASLLGTSWDKGIFSWACVNHELDNKHSFPTFSRTLPSPIRVLVTVMKYFQWAHAGVISSDEDIWMHTANRVSSALRSQGLPVGVVLTSGRDSQSIQKALQQIRQADRIRIIIMCMHSALIGGETQTHFLELAHDLKMTDGTYVFVPYDVLLYSLPYKHSPYQVLRNNPKLREAYDAVLTITVESHEKTFYEAYAEAAARGEIPEKPDSNQVSPLFGTIYNSIYFIAQAMNNAMKKNGRASAASLVQHSRNMQFYGFNQLIKTDSNGNGISEYVILDTNGKEWELRGTYTVDMETELLRFRGTPIHFPGGRPTSADAKCWFAERKICQGGIDPALAMMVCFALLIALLSINGFAYFIRRRINKIQLIKGPNRILLTLEDVTFINPHFGSKRGSRASVSFQIISEVQSGRSPRLSFSSGSLTPATYENSNIAIYEGDWVWLKKFPPGDFGDIKSIKSSASDVFEMMKDLRHENVNPLLGFFYDSGMFAIVSEFCSRRSLEDILTNDDVKLDWMFKSSLLLDLIKGMKYLHHREFIHGRLKSRNCVVDGRFVLKVTDYGFNDILEMLRLSEEEPSEEELLWTAPELLRAPGGIRLGSFAGDVYSFAIIMQEVMVRGAPFCMMDLPAKEIIDRLKMPPPVYRPVVSPEYAPAECLQLMKQCWAEASEQRPTFDEIFNQFKTFNKGKKTNIIDSMLRMLEQYSSNLEDLIRERTEELEIEKQKTEKLLTQMLPLSVAESLKKGCTVEPEGFDLVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPKRNGSRHAAEIANMSLDILSSVGTFKMRHMPEVPVRIRIGLHSGPVVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSLSTVTILQTLSEGYEVELRGRTELKGKGTEETFWLVGKKGFTKPLPVPPPVGKDGQVGHGLQPAEIAAFQRRKAERQLVRNKP

Target ORF information:

RefSeq Version	NM_001007576.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanylate cyclase 2f (Gucy2f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007576.2

ORF Insert Sequence:

ATGTTCCTCG GACCCTGGCC TTTTTCTCGC CTTCTGTCCT GGTTTGCCAT TTCCAGCAGA 
CTGTCAGGAC AGCATGGCCT CCCTTCCTCC AAGTTCCTGA GATGTTTGTG CCTCTTGGCC 
CTTTTGCCTC TCCTTCGGTG GGGGCAAGCA CTCCCCTACA AGATCGGGGT CATAGGTCCC 
TGGACATGTG ATCCGTTCTT TTCCAAGGCC CTGCCTGAGG TTGCTGCTGC TTTAGCAATT 
GAGCGAATCA GCCGGGACAA GACATTTGAC AGGAGTTACT CCTTCGAATA CGTGATTCTT 
AACGAAGACT GTCAGACTTC AAAGGCCCTC GCCAGTTTCA TTTCCCACCA GCAGATGGCC 
TCAGGATTTG TTGGGCCTGC CAACCCTGGC TTCTGCGAGG CAGCCTCACT CCTGGGAACC 
AGCTGGGACA AAGGGATTTT CTCTTGGGCT TGTGTGAATC ATGAATTAGA TAACAAACAT 
AGCTTCCCAA CCTTCTCTCG GACACTCCCT TCTCCGATAC GGGTGCTTGT AACCGTCATG 
AAGTATTTCC AGTGGGCACA TGCTGGGGTC ATTTCCTCAG ATGAAGACAT TTGGATGCAT 
ACAGCCAATC GAGTCTCAAG TGCTCTTCGG AGCCAGGGCC TACCTGTAGG GGTCGTCCTG 
ACCTCGGGAC GAGACAGCCA GAGCATTCAG AAAGCTCTCC AGCAGATTCG CCAGGCAGAC 
AGAATTCGCA TAATCATAAT GTGTATGCAT TCAGCCTTGA TTGGGGGAGA GACACAAACA 
CACTTCTTGG AATTGGCTCA TGATCTGAAA ATGACAGATG GGACTTACGT CTTTGTCCCC 
TATGATGTAC TGCTCTACAG TTTACCTTAT AAGCACAGCC CCTACCAGGT CCTGAGGAAC 
AATCCAAAGC TCCGGGAAGC CTATGATGCT GTGTTGACTA TTACAGTGGA GTCCCATGAA 
AAGACCTTCT ACGAAGCCTA TGCAGAAGCA GCAGCCCGCG GAGAAATTCC TGAGAAGCCT 
GACTCAAACC AAGTTTCACC GTTGTTTGGA ACCATCTACA ATTCAATTTA TTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAAAAAAT GGACGGGCTA GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CTATGGATTC AACCAGTTGA TAAAGACAGA CTCAAATGGA 
AATGGGATTT CTGAATATGT AATCCTGGAC ACCAATGGGA AAGAATGGGA ACTTCGTGGC 
ACCTACACTG TGGACATGGA AACTGAACTG CTAAGGTTCA GAGGGACCCC AATTCATTTC 
CCTGGCGGCA GACCTACTAG TGCAGATGCA AAATGCTGGT TTGCAGAAAG GAAGATCTGC 
CAAGGAGGTA TTGACCCTGC CTTGGCCATG ATGGTCTGCT TTGCTTTACT TATAGCCTTG 
CTGTCTATCA ATGGATTTGC TTACTTTATA AGGCGACGTA TAAATAAAAT CCAGTTGATT 
AAAGGACCCA ACAGAATCCT ACTGACTTTG GAAGATGTGA CATTTATTAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGCCG TGCCAGTGTA AGCTTCCAGA TCATCTCAGA AGTTCAAAGT 
GGGAGGTCCC CAAGGCTGTC CTTTTCTTCA GGTAGTCTAA CTCCAGCTAC ATATGAGAAC 
TCCAACATAG CAATCTATGA GGGTGACTGG GTATGGCTGA AAAAGTTCCC TCCTGGAGAT 
TTTGGAGACA TTAAGTCTAT CAAATCGAGT GCAAGTGATG TGTTTGAAAT GATGAAGGAC 
CTGCGGCATG AAAATGTCAA CCCTCTATTG GGTTTCTTCT ATGATTCGGG GATGTTTGCC 
ATCGTGTCAG AATTTTGTTC TCGAAGGAGC CTAGAAGACA TACTTACAAA TGATGATGTG 
AAACTTGATT GGATGTTCAA ATCATCACTA CTGCTTGACC TCATCAAGGG GATGAAATAC 
TTACACCACA GAGAATTTAT TCACGGGAGG CTAAAGTCTC GGAACTGTGT GGTAGATGGG 
CGTTTTGTCC TAAAAGTGAC AGATTATGGC TTTAATGACA TTTTAGAAAT GCTGAGACTC 
TCTGAAGAGG AACCTTCTGA AGAAGAGCTG CTGTGGACGG CCCCTGAACT GTTAAGAGCC 
CCTGGGGGCA TCAGGTTAGG TTCATTTGCA GGAGACGTCT ATAGCTTTGC CATCATCATG 
CAAGAAGTGA TGGTTCGGGG TGCTCCTTTC TGCATGATGG ATCTGCCTGC CAAAGAAATC 
ATAGACAGAC TTAAAATGCC TCCCCCTGTG TACAGACCCG TGGTTTCTCC TGAGTATGCC 
CCTGCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCGTCAGA ACAGCGACCA 
ACTTTTGATG AAATATTTAA CCAGTTTAAA ACTTTCAACA AAGGGAAAAA GACTAATATT 
ATTGATTCTA TGCTTCGGAT GTTGGAGCAG TATTCTAGCA ACTTGGAAGA TTTGATCCGG 
GAACGGACTG AAGAATTGGA AATTGAAAAA CAGAAAACGG AAAAGCTTCT AACACAGATG 
CTACCACTAT CAGTTGCTGA ATCCCTCAAG AAGGGCTGTA CAGTTGAACC TGAGGGATTT 
GACTTGGTCA CCTTGTACTT CAGTGACATT GTGGGCTTCA CCACCATCTC AGCCATGAGT 
GAGCCCATTG AAGTTGTGGA CCTTCTGAAT GACCTGTACA CACTCTTTGA TGCCATCATT 
GGCAGTCATG ATGTCTATAA AGTAGAGACC ATTGGAGATG CCTACATGGT GGCCTCAGGC 
CTTCCCAAGA GGAATGGCAG TAGGCATGCA GCTGAGATTG CAAACATGTC CCTGGATATC 
CTGAGTTCTG TGGGTACCTT TAAGATGCGA CACATGCCAG AGGTGCCAGT CCGAATTCGA 
ATAGGCCTGC ACTCGGGGCC TGTTGTTGCT GGAGTGGTGG GCCTCACCAT GCCCAGATAT 
TGCTTGTTTG GGGACACTGT GAACACAGCT TCTCGAATGG AATCAACAGG GCTACCATAC 
CGAATTCATG TCAGTCTCAG CACAGTAACA ATTCTTCAAA CTCTGAGTGA AGGCTATGAA 
GTAGAGCTTC GTGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAGGAGAC CTTTTGGCTT 
GTTGGAAAAA AGGGTTTCAC CAAACCTCTT CCTGTACCCC CACCAGTGGG CAAAGATGGG 
CAAGTGGGCC ATGGCTTGCA ACCAGCAGAG ATTGCAGCAT TCCAAAGGAG AAAAGCAGAG 
AGGCAGTTGG TGCGAAACAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy2f ( XM_006528841.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528904.1 Mus musculus guanylate cyclase 2f (Gucy2f), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu10777
Clone ID Related Accession (Same CDS sequence)	XM_006528841.3 , XM_006528841.2 , XM_006528841.4 , NM_001007576.2
Accession Version	XM_006528841.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	retinal guanylyl cyclase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528841.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCG GACCCTGGCC TTTTTCTCGC CTTCTGTCCT GGTTTGCCAT TTCCAGCAGA 
CTGTCAGGAC AGCATGGCCT CCCTTCCTCC AAGTTCCTGA GATGTTTGTG CCTCTTGGCC 
CTTTTGCCTC TCCTTCGGTG GGGGCAAGCA CTCCCCTACA AGATCGGGGT CATAGGTCCC 
TGGACATGTG ATCCGTTCTT TTCCAAGGCC CTGCCTGAGG TTGCTGCTGC TTTAGCAATT 
GAGCGAATCA GCCGGGACAA GACATTTGAC AGGAGTTACT CCTTCGAATA CGTGATTCTT 
AACGAAGACT GTCAGACTTC AAAGGCCCTC GCCAGTTTCA TTTCCCACCA GCAGATGGCC 
TCAGGATTTG TTGGGCCTGC CAACCCTGGC TTCTGCGAGG CAGCCTCACT CCTGGGAACC 
AGCTGGGACA AAGGGATTTT CTCTTGGGCT TGTGTGAATC ATGAATTAGA TAACAAACAT 
AGCTTCCCAA CCTTCTCTCG GACACTCCCT TCTCCGATAC GGGTGCTTGT AACCGTCATG 
AAGTATTTCC AGTGGGCACA TGCTGGGGTC ATTTCCTCAG ATGAAGACAT TTGGATGCAT 
ACAGCCAATC GAGTCTCAAG TGCTCTTCGG AGCCAGGGCC TACCTGTAGG GGTCGTCCTG 
ACCTCGGGAC GAGACAGCCA GAGCATTCAG AAAGCTCTCC AGCAGATTCG CCAGGCAGAC 
AGAATTCGCA TAATCATAAT GTGTATGCAT TCAGCCTTGA TTGGGGGAGA GACACAAACA 
CACTTCTTGG AATTGGCTCA TGATCTGAAA ATGACAGATG GGACTTACGT CTTTGTCCCC 
TATGATGTAC TGCTCTACAG TTTACCTTAT AAGCACAGCC CCTACCAGGT CCTGAGGAAC 
AATCCAAAGC TCCGGGAAGC CTATGATGCT GTGTTGACTA TTACAGTGGA GTCCCATGAA 
AAGACCTTCT ACGAAGCCTA TGCAGAAGCA GCAGCCCGCG GAGAAATTCC TGAGAAGCCT 
GACTCAAACC AAGTTTCACC GTTGTTTGGA ACCATCTACA ATTCAATTTA TTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAAAAAAT GGACGGGCTA GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CTATGGATTC AACCAGTTGA TAAAGACAGA CTCAAATGGA 
AATGGGATTT CTGAATATGT AATCCTGGAC ACCAATGGGA AAGAATGGGA ACTTCGTGGC 
ACCTACACTG TGGACATGGA AACTGAACTG CTAAGGTTCA GAGGGACCCC AATTCATTTC 
CCTGGCGGCA GACCTACTAG TGCAGATGCA AAATGCTGGT TTGCAGAAAG GAAGATCTGC 
CAAGGAGGTA TTGACCCTGC CTTGGCCATG ATGGTCTGCT TTGCTTTACT TATAGCCTTG 
CTGTCTATCA ATGGATTTGC TTACTTTATA AGGCGACGTA TAAATAAAAT CCAGTTGATT 
AAAGGACCCA ACAGAATCCT ACTGACTTTG GAAGATGTGA CATTTATTAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGCCG TGCCAGTGTA AGCTTCCAGA TCATCTCAGA AGTTCAAAGT 
GGGAGGTCCC CAAGGCTGTC CTTTTCTTCA GGTAGTCTAA CTCCAGCTAC ATATGAGAAC 
TCCAACATAG CAATCTATGA GGGTGACTGG GTATGGCTGA AAAAGTTCCC TCCTGGAGAT 
TTTGGAGACA TTAAGTCTAT CAAATCGAGT GCAAGTGATG TGTTTGAAAT GATGAAGGAC 
CTGCGGCATG AAAATGTCAA CCCTCTATTG GGTTTCTTCT ATGATTCGGG GATGTTTGCC 
ATCGTGTCAG AATTTTGTTC TCGAAGGAGC CTAGAAGACA TACTTACAAA TGATGATGTG 
AAACTTGATT GGATGTTCAA ATCATCACTA CTGCTTGACC TCATCAAGGG GATGAAATAC 
TTACACCACA GAGAATTTAT TCACGGGAGG CTAAAGTCTC GGAACTGTGT GGTAGATGGG 
CGTTTTGTCC TAAAAGTGAC AGATTATGGC TTTAATGACA TTTTAGAAAT GCTGAGACTC 
TCTGAAGAGG AACCTTCTGA AGAAGAGCTG CTGTGGACGG CCCCTGAACT GTTAAGAGCC 
CCTGGGGGCA TCAGGTTAGG TTCATTTGCA GGAGACGTCT ATAGCTTTGC CATCATCATG 
CAAGAAGTGA TGGTTCGGGG TGCTCCTTTC TGCATGATGG ATCTGCCTGC CAAAGAAATC 
ATAGACAGAC TTAAAATGCC TCCCCCTGTG TACAGACCCG TGGTTTCTCC TGAGTATGCC 
CCTGCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCGTCAGA ACAGCGACCA 
ACTTTTGATG AAATATTTAA CCAGTTTAAA ACTTTCAACA AAGGGAAAAA GACTAATATT 
ATTGATTCTA TGCTTCGGAT GTTGGAGCAG TATTCTAGCA ACTTGGAAGA TTTGATCCGG 
GAACGGACTG AAGAATTGGA AATTGAAAAA CAGAAAACGG AAAAGCTTCT AACACAGATG 
CTACCACTAT CAGTTGCTGA ATCCCTCAAG AAGGGCTGTA CAGTTGAACC TGAGGGATTT 
GACTTGGTCA CCTTGTACTT CAGTGACATT GTGGGCTTCA CCACCATCTC AGCCATGAGT 
GAGCCCATTG AAGTTGTGGA CCTTCTGAAT GACCTGTACA CACTCTTTGA TGCCATCATT 
GGCAGTCATG ATGTCTATAA AGTAGAGACC ATTGGAGATG CCTACATGGT GGCCTCAGGC 
CTTCCCAAGA GGAATGGCAG TAGGCATGCA GCTGAGATTG CAAACATGTC CCTGGATATC 
CTGAGTTCTG TGGGTACCTT TAAGATGCGA CACATGCCAG AGGTGCCAGT CCGAATTCGA 
ATAGGCCTGC ACTCGGGGCC TGTTGTTGCT GGAGTGGTGG GCCTCACCAT GCCCAGATAT 
TGCTTGTTTG GGGACACTGT GAACACAGCT TCTCGAATGG AATCAACAGG GCTACCATAC 
CGAATTCATG TCAGTCTCAG CACAGTAACA ATTCTTCAAA CTCTGAGTGA AGGCTATGAA 
GTAGAGCTTC GTGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAGGAGAC CTTTTGGCTT 
GTTGGAAAAA AGGGTTTCAC CAAACCTCTT CCTGTACCCC CACCAGTGGG CAAAGATGGG 
CAAGTGGGCC ATGGCTTGCA ACCAGCAGAG ATTGCAGCAT TCCAAAGGAG AAAAGCAGAG 
AGGCAGTTGG TGCGAAACAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528904.1
CDS	395..3721
Translation	MFLGPWPFSRLLSWFAISSRLSGQHGLPSSKFLRCLCLLALLPLLRWGQALPYKIGVIGPWTCDPFFSKALPEVAAALAIERISRDKTFDRSYSFEYVILNEDCQTSKALASFISHQQMASGFVGPANPGFCEAASLLGTSWDKGIFSWACVNHELDNKHSFPTFSRTLPSPIRVLVTVMKYFQWAHAGVISSDEDIWMHTANRVSSALRSQGLPVGVVLTSGRDSQSIQKALQQIRQADRIRIIIMCMHSALIGGETQTHFLELAHDLKMTDGTYVFVPYDVLLYSLPYKHSPYQVLRNNPKLREAYDAVLTITVESHEKTFYEAYAEAAARGEIPEKPDSNQVSPLFGTIYNSIYFIAQAMNNAMKKNGRASAASLVQHSRNMQFYGFNQLIKTDSNGNGISEYVILDTNGKEWELRGTYTVDMETELLRFRGTPIHFPGGRPTSADAKCWFAERKICQGGIDPALAMMVCFALLIALLSINGFAYFIRRRINKIQLIKGPNRILLTLEDVTFINPHFGSKRGSRASVSFQIISEVQSGRSPRLSFSSGSLTPATYENSNIAIYEGDWVWLKKFPPGDFGDIKSIKSSASDVFEMMKDLRHENVNPLLGFFYDSGMFAIVSEFCSRRSLEDILTNDDVKLDWMFKSSLLLDLIKGMKYLHHREFIHGRLKSRNCVVDGRFVLKVTDYGFNDILEMLRLSEEEPSEEELLWTAPELLRAPGGIRLGSFAGDVYSFAIIMQEVMVRGAPFCMMDLPAKEIIDRLKMPPPVYRPVVSPEYAPAECLQLMKQCWAEASEQRPTFDEIFNQFKTFNKGKKTNIIDSMLRMLEQYSSNLEDLIRERTEELEIEKQKTEKLLTQMLPLSVAESLKKGCTVEPEGFDLVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPKRNGSRHAAEIANMSLDILSSVGTFKMRHMPEVPVRIRIGLHSGPVVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSLSTVTILQTLSEGYEVELRGRTELKGKGTEETFWLVGKKGFTKPLPVPPPVGKDGQVGHGLQPAEIAAFQRRKAERQLVRNKP

Target ORF information:

RefSeq Version	XM_006528841.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanylate cyclase 2f (Gucy2f), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528841.3

ORF Insert Sequence:

ATGTTCCTCG GACCCTGGCC TTTTTCTCGC CTTCTGTCCT GGTTTGCCAT TTCCAGCAGA 
CTGTCAGGAC AGCATGGCCT CCCTTCCTCC AAGTTCCTGA GATGTTTGTG CCTCTTGGCC 
CTTTTGCCTC TCCTTCGGTG GGGGCAAGCA CTCCCCTACA AGATCGGGGT CATAGGTCCC 
TGGACATGTG ATCCGTTCTT TTCCAAGGCC CTGCCTGAGG TTGCTGCTGC TTTAGCAATT 
GAGCGAATCA GCCGGGACAA GACATTTGAC AGGAGTTACT CCTTCGAATA CGTGATTCTT 
AACGAAGACT GTCAGACTTC AAAGGCCCTC GCCAGTTTCA TTTCCCACCA GCAGATGGCC 
TCAGGATTTG TTGGGCCTGC CAACCCTGGC TTCTGCGAGG CAGCCTCACT CCTGGGAACC 
AGCTGGGACA AAGGGATTTT CTCTTGGGCT TGTGTGAATC ATGAATTAGA TAACAAACAT 
AGCTTCCCAA CCTTCTCTCG GACACTCCCT TCTCCGATAC GGGTGCTTGT AACCGTCATG 
AAGTATTTCC AGTGGGCACA TGCTGGGGTC ATTTCCTCAG ATGAAGACAT TTGGATGCAT 
ACAGCCAATC GAGTCTCAAG TGCTCTTCGG AGCCAGGGCC TACCTGTAGG GGTCGTCCTG 
ACCTCGGGAC GAGACAGCCA GAGCATTCAG AAAGCTCTCC AGCAGATTCG CCAGGCAGAC 
AGAATTCGCA TAATCATAAT GTGTATGCAT TCAGCCTTGA TTGGGGGAGA GACACAAACA 
CACTTCTTGG AATTGGCTCA TGATCTGAAA ATGACAGATG GGACTTACGT CTTTGTCCCC 
TATGATGTAC TGCTCTACAG TTTACCTTAT AAGCACAGCC CCTACCAGGT CCTGAGGAAC 
AATCCAAAGC TCCGGGAAGC CTATGATGCT GTGTTGACTA TTACAGTGGA GTCCCATGAA 
AAGACCTTCT ACGAAGCCTA TGCAGAAGCA GCAGCCCGCG GAGAAATTCC TGAGAAGCCT 
GACTCAAACC AAGTTTCACC GTTGTTTGGA ACCATCTACA ATTCAATTTA TTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAAAAAAT GGACGGGCTA GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CTATGGATTC AACCAGTTGA TAAAGACAGA CTCAAATGGA 
AATGGGATTT CTGAATATGT AATCCTGGAC ACCAATGGGA AAGAATGGGA ACTTCGTGGC 
ACCTACACTG TGGACATGGA AACTGAACTG CTAAGGTTCA GAGGGACCCC AATTCATTTC 
CCTGGCGGCA GACCTACTAG TGCAGATGCA AAATGCTGGT TTGCAGAAAG GAAGATCTGC 
CAAGGAGGTA TTGACCCTGC CTTGGCCATG ATGGTCTGCT TTGCTTTACT TATAGCCTTG 
CTGTCTATCA ATGGATTTGC TTACTTTATA AGGCGACGTA TAAATAAAAT CCAGTTGATT 
AAAGGACCCA ACAGAATCCT ACTGACTTTG GAAGATGTGA CATTTATTAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGCCG TGCCAGTGTA AGCTTCCAGA TCATCTCAGA AGTTCAAAGT 
GGGAGGTCCC CAAGGCTGTC CTTTTCTTCA GGTAGTCTAA CTCCAGCTAC ATATGAGAAC 
TCCAACATAG CAATCTATGA GGGTGACTGG GTATGGCTGA AAAAGTTCCC TCCTGGAGAT 
TTTGGAGACA TTAAGTCTAT CAAATCGAGT GCAAGTGATG TGTTTGAAAT GATGAAGGAC 
CTGCGGCATG AAAATGTCAA CCCTCTATTG GGTTTCTTCT ATGATTCGGG GATGTTTGCC 
ATCGTGTCAG AATTTTGTTC TCGAAGGAGC CTAGAAGACA TACTTACAAA TGATGATGTG 
AAACTTGATT GGATGTTCAA ATCATCACTA CTGCTTGACC TCATCAAGGG GATGAAATAC 
TTACACCACA GAGAATTTAT TCACGGGAGG CTAAAGTCTC GGAACTGTGT GGTAGATGGG 
CGTTTTGTCC TAAAAGTGAC AGATTATGGC TTTAATGACA TTTTAGAAAT GCTGAGACTC 
TCTGAAGAGG AACCTTCTGA AGAAGAGCTG CTGTGGACGG CCCCTGAACT GTTAAGAGCC 
CCTGGGGGCA TCAGGTTAGG TTCATTTGCA GGAGACGTCT ATAGCTTTGC CATCATCATG 
CAAGAAGTGA TGGTTCGGGG TGCTCCTTTC TGCATGATGG ATCTGCCTGC CAAAGAAATC 
ATAGACAGAC TTAAAATGCC TCCCCCTGTG TACAGACCCG TGGTTTCTCC TGAGTATGCC 
CCTGCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCGTCAGA ACAGCGACCA 
ACTTTTGATG AAATATTTAA CCAGTTTAAA ACTTTCAACA AAGGGAAAAA GACTAATATT 
ATTGATTCTA TGCTTCGGAT GTTGGAGCAG TATTCTAGCA ACTTGGAAGA TTTGATCCGG 
GAACGGACTG AAGAATTGGA AATTGAAAAA CAGAAAACGG AAAAGCTTCT AACACAGATG 
CTACCACTAT CAGTTGCTGA ATCCCTCAAG AAGGGCTGTA CAGTTGAACC TGAGGGATTT 
GACTTGGTCA CCTTGTACTT CAGTGACATT GTGGGCTTCA CCACCATCTC AGCCATGAGT 
GAGCCCATTG AAGTTGTGGA CCTTCTGAAT GACCTGTACA CACTCTTTGA TGCCATCATT 
GGCAGTCATG ATGTCTATAA AGTAGAGACC ATTGGAGATG CCTACATGGT GGCCTCAGGC 
CTTCCCAAGA GGAATGGCAG TAGGCATGCA GCTGAGATTG CAAACATGTC CCTGGATATC 
CTGAGTTCTG TGGGTACCTT TAAGATGCGA CACATGCCAG AGGTGCCAGT CCGAATTCGA 
ATAGGCCTGC ACTCGGGGCC TGTTGTTGCT GGAGTGGTGG GCCTCACCAT GCCCAGATAT 
TGCTTGTTTG GGGACACTGT GAACACAGCT TCTCGAATGG AATCAACAGG GCTACCATAC 
CGAATTCATG TCAGTCTCAG CACAGTAACA ATTCTTCAAA CTCTGAGTGA AGGCTATGAA 
GTAGAGCTTC GTGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAGGAGAC CTTTTGGCTT 
GTTGGAAAAA AGGGTTTCAC CAAACCTCTT CCTGTACCCC CACCAGTGGG CAAAGATGGG 
CAAGTGGGCC ATGGCTTGCA ACCAGCAGAG ATTGCAGCAT TCCAAAGGAG AAAAGCAGAG 
AGGCAGTTGG TGCGAAACAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy2f ( XM_006528841.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528904.1 Mus musculus guanylate cyclase 2f (Gucy2f), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu10777
Clone ID Related Accession (Same CDS sequence)	XM_006528841.3 , XM_006528841.2 , XM_006528841.4 , NM_001007576.2
Accession Version	XM_006528841.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	retinal guanylyl cyclase 2 isoform X1
Comment

ATGTTCCTCG GACCCTGGCC TTTTTCTCGC CTTCTGTCCT GGTTTGCCAT TTCCAGCAGA 
CTGTCAGGAC AGCATGGCCT CCCTTCCTCC AAGTTCCTGA GATGTTTGTG CCTCTTGGCC 
CTTTTGCCTC TCCTTCGGTG GGGGCAAGCA CTCCCCTACA AGATCGGGGT CATAGGTCCC 
TGGACATGTG ATCCGTTCTT TTCCAAGGCC CTGCCTGAGG TTGCTGCTGC TTTAGCAATT 
GAGCGAATCA GCCGGGACAA GACATTTGAC AGGAGTTACT CCTTCGAATA CGTGATTCTT 
AACGAAGACT GTCAGACTTC AAAGGCCCTC GCCAGTTTCA TTTCCCACCA GCAGATGGCC 
TCAGGATTTG TTGGGCCTGC CAACCCTGGC TTCTGCGAGG CAGCCTCACT CCTGGGAACC 
AGCTGGGACA AAGGGATTTT CTCTTGGGCT TGTGTGAATC ATGAATTAGA TAACAAACAT 
AGCTTCCCAA CCTTCTCTCG GACACTCCCT TCTCCGATAC GGGTGCTTGT AACCGTCATG 
AAGTATTTCC AGTGGGCACA TGCTGGGGTC ATTTCCTCAG ATGAAGACAT TTGGATGCAT 
ACAGCCAATC GAGTCTCAAG TGCTCTTCGG AGCCAGGGCC TACCTGTAGG GGTCGTCCTG 
ACCTCGGGAC GAGACAGCCA GAGCATTCAG AAAGCTCTCC AGCAGATTCG CCAGGCAGAC 
AGAATTCGCA TAATCATAAT GTGTATGCAT TCAGCCTTGA TTGGGGGAGA GACACAAACA 
CACTTCTTGG AATTGGCTCA TGATCTGAAA ATGACAGATG GGACTTACGT CTTTGTCCCC 
TATGATGTAC TGCTCTACAG TTTACCTTAT AAGCACAGCC CCTACCAGGT CCTGAGGAAC 
AATCCAAAGC TCCGGGAAGC CTATGATGCT GTGTTGACTA TTACAGTGGA GTCCCATGAA 
AAGACCTTCT ACGAAGCCTA TGCAGAAGCA GCAGCCCGCG GAGAAATTCC TGAGAAGCCT 
GACTCAAACC AAGTTTCACC GTTGTTTGGA ACCATCTACA ATTCAATTTA TTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAAAAAAT GGACGGGCTA GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CTATGGATTC AACCAGTTGA TAAAGACAGA CTCAAATGGA 
AATGGGATTT CTGAATATGT AATCCTGGAC ACCAATGGGA AAGAATGGGA ACTTCGTGGC 
ACCTACACTG TGGACATGGA AACTGAACTG CTAAGGTTCA GAGGGACCCC AATTCATTTC 
CCTGGCGGCA GACCTACTAG TGCAGATGCA AAATGCTGGT TTGCAGAAAG GAAGATCTGC 
CAAGGAGGTA TTGACCCTGC CTTGGCCATG ATGGTCTGCT TTGCTTTACT TATAGCCTTG 
CTGTCTATCA ATGGATTTGC TTACTTTATA AGGCGACGTA TAAATAAAAT CCAGTTGATT 
AAAGGACCCA ACAGAATCCT ACTGACTTTG GAAGATGTGA CATTTATTAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGCCG TGCCAGTGTA AGCTTCCAGA TCATCTCAGA AGTTCAAAGT 
GGGAGGTCCC CAAGGCTGTC CTTTTCTTCA GGTAGTCTAA CTCCAGCTAC ATATGAGAAC 
TCCAACATAG CAATCTATGA GGGTGACTGG GTATGGCTGA AAAAGTTCCC TCCTGGAGAT 
TTTGGAGACA TTAAGTCTAT CAAATCGAGT GCAAGTGATG TGTTTGAAAT GATGAAGGAC 
CTGCGGCATG AAAATGTCAA CCCTCTATTG GGTTTCTTCT ATGATTCGGG GATGTTTGCC 
ATCGTGTCAG AATTTTGTTC TCGAAGGAGC CTAGAAGACA TACTTACAAA TGATGATGTG 
AAACTTGATT GGATGTTCAA ATCATCACTA CTGCTTGACC TCATCAAGGG GATGAAATAC 
TTACACCACA GAGAATTTAT TCACGGGAGG CTAAAGTCTC GGAACTGTGT GGTAGATGGG 
CGTTTTGTCC TAAAAGTGAC AGATTATGGC TTTAATGACA TTTTAGAAAT GCTGAGACTC 
TCTGAAGAGG AACCTTCTGA AGAAGAGCTG CTGTGGACGG CCCCTGAACT GTTAAGAGCC 
CCTGGGGGCA TCAGGTTAGG TTCATTTGCA GGAGACGTCT ATAGCTTTGC CATCATCATG 
CAAGAAGTGA TGGTTCGGGG TGCTCCTTTC TGCATGATGG ATCTGCCTGC CAAAGAAATC 
ATAGACAGAC TTAAAATGCC TCCCCCTGTG TACAGACCCG TGGTTTCTCC TGAGTATGCC 
CCTGCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCGTCAGA ACAGCGACCA 
ACTTTTGATG AAATATTTAA CCAGTTTAAA ACTTTCAACA AAGGGAAAAA GACTAATATT 
ATTGATTCTA TGCTTCGGAT GTTGGAGCAG TATTCTAGCA ACTTGGAAGA TTTGATCCGG 
GAACGGACTG AAGAATTGGA AATTGAAAAA CAGAAAACGG AAAAGCTTCT AACACAGATG 
CTACCACTAT CAGTTGCTGA ATCCCTCAAG AAGGGCTGTA CAGTTGAACC TGAGGGATTT 
GACTTGGTCA CCTTGTACTT CAGTGACATT GTGGGCTTCA CCACCATCTC AGCCATGAGT 
GAGCCCATTG AAGTTGTGGA CCTTCTGAAT GACCTGTACA CACTCTTTGA TGCCATCATT 
GGCAGTCATG ATGTCTATAA AGTAGAGACC ATTGGAGATG CCTACATGGT GGCCTCAGGC 
CTTCCCAAGA GGAATGGCAG TAGGCATGCA GCTGAGATTG CAAACATGTC CCTGGATATC 
CTGAGTTCTG TGGGTACCTT TAAGATGCGA CACATGCCAG AGGTGCCAGT CCGAATTCGA 
ATAGGCCTGC ACTCGGGGCC TGTTGTTGCT GGAGTGGTGG GCCTCACCAT GCCCAGATAT 
TGCTTGTTTG GGGACACTGT GAACACAGCT TCTCGAATGG AATCAACAGG GCTACCATAC 
CGAATTCATG TCAGTCTCAG CACAGTAACA ATTCTTCAAA CTCTGAGTGA AGGCTATGAA 
GTAGAGCTTC GTGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAGGAGAC CTTTTGGCTT 
GTTGGAAAAA AGGGTTTCAC CAAACCTCTT CCTGTACCCC CACCAGTGGG CAAAGATGGG 
CAAGTGGGCC ATGGCTTGCA ACCAGCAGAG ATTGCAGCAT TCCAAAGGAG AAAAGCAGAG 
AGGCAGTTGG TGCGAAACAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006528904.1

Translation

MFLGPWPFSRLLSWFAISSRLSGQHGLPSSKFLRCLCLLALLPLLRWGQALPYKIGVIGPWTCDPFFSKALPEVAAALAIERISRDKTFDRSYSFEYVILNEDCQTSKALASFISHQQMASGFVGPANPGFCEAASLLGTSWDKGIFSWACVNHELDNKHSFPTFSRTLPSPIRVLVTVMKYFQWAHAGVISSDEDIWMHTANRVSSALRSQGLPVGVVLTSGRDSQSIQKALQQIRQADRIRIIIMCMHSALIGGETQTHFLELAHDLKMTDGTYVFVPYDVLLYSLPYKHSPYQVLRNNPKLREAYDAVLTITVESHEKTFYEAYAEAAARGEIPEKPDSNQVSPLFGTIYNSIYFIAQAMNNAMKKNGRASAASLVQHSRNMQFYGFNQLIKTDSNGNGISEYVILDTNGKEWELRGTYTVDMETELLRFRGTPIHFPGGRPTSADAKCWFAERKICQGGIDPALAMMVCFALLIALLSINGFAYFIRRRINKIQLIKGPNRILLTLEDVTFINPHFGSKRGSRASVSFQIISEVQSGRSPRLSFSSGSLTPATYENSNIAIYEGDWVWLKKFPPGDFGDIKSIKSSASDVFEMMKDLRHENVNPLLGFFYDSGMFAIVSEFCSRRSLEDILTNDDVKLDWMFKSSLLLDLIKGMKYLHHREFIHGRLKSRNCVVDGRFVLKVTDYGFNDILEMLRLSEEEPSEEELLWTAPELLRAPGGIRLGSFAGDVYSFAIIMQEVMVRGAPFCMMDLPAKEIIDRLKMPPPVYRPVVSPEYAPAECLQLMKQCWAEASEQRPTFDEIFNQFKTFNKGKKTNIIDSMLRMLEQYSSNLEDLIRERTEELEIEKQKTEKLLTQMLPLSVAESLKKGCTVEPEGFDLVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPKRNGSRHAAEIANMSLDILSSVGTFKMRHMPEVPVRIRIGLHSGPVVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSLSTVTILQTLSEGYEVELRGRTELKGKGTEETFWLVGKKGFTKPLPVPPPVGKDGQVGHGLQPAEIAAFQRRKAERQLVRNKP

Target ORF information:

RefSeq Version	XM_006528841.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus guanylate cyclase 2f (Gucy2f), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528841.2

ORF Insert Sequence:

ATGTTCCTCG GACCCTGGCC TTTTTCTCGC CTTCTGTCCT GGTTTGCCAT TTCCAGCAGA 
CTGTCAGGAC AGCATGGCCT CCCTTCCTCC AAGTTCCTGA GATGTTTGTG CCTCTTGGCC 
CTTTTGCCTC TCCTTCGGTG GGGGCAAGCA CTCCCCTACA AGATCGGGGT CATAGGTCCC 
TGGACATGTG ATCCGTTCTT TTCCAAGGCC CTGCCTGAGG TTGCTGCTGC TTTAGCAATT 
GAGCGAATCA GCCGGGACAA GACATTTGAC AGGAGTTACT CCTTCGAATA CGTGATTCTT 
AACGAAGACT GTCAGACTTC AAAGGCCCTC GCCAGTTTCA TTTCCCACCA GCAGATGGCC 
TCAGGATTTG TTGGGCCTGC CAACCCTGGC TTCTGCGAGG CAGCCTCACT CCTGGGAACC 
AGCTGGGACA AAGGGATTTT CTCTTGGGCT TGTGTGAATC ATGAATTAGA TAACAAACAT 
AGCTTCCCAA CCTTCTCTCG GACACTCCCT TCTCCGATAC GGGTGCTTGT AACCGTCATG 
AAGTATTTCC AGTGGGCACA TGCTGGGGTC ATTTCCTCAG ATGAAGACAT TTGGATGCAT 
ACAGCCAATC GAGTCTCAAG TGCTCTTCGG AGCCAGGGCC TACCTGTAGG GGTCGTCCTG 
ACCTCGGGAC GAGACAGCCA GAGCATTCAG AAAGCTCTCC AGCAGATTCG CCAGGCAGAC 
AGAATTCGCA TAATCATAAT GTGTATGCAT TCAGCCTTGA TTGGGGGAGA GACACAAACA 
CACTTCTTGG AATTGGCTCA TGATCTGAAA ATGACAGATG GGACTTACGT CTTTGTCCCC 
TATGATGTAC TGCTCTACAG TTTACCTTAT AAGCACAGCC CCTACCAGGT CCTGAGGAAC 
AATCCAAAGC TCCGGGAAGC CTATGATGCT GTGTTGACTA TTACAGTGGA GTCCCATGAA 
AAGACCTTCT ACGAAGCCTA TGCAGAAGCA GCAGCCCGCG GAGAAATTCC TGAGAAGCCT 
GACTCAAACC AAGTTTCACC GTTGTTTGGA ACCATCTACA ATTCAATTTA TTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAAAAAAT GGACGGGCTA GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CTATGGATTC AACCAGTTGA TAAAGACAGA CTCAAATGGA 
AATGGGATTT CTGAATATGT AATCCTGGAC ACCAATGGGA AAGAATGGGA ACTTCGTGGC 
ACCTACACTG TGGACATGGA AACTGAACTG CTAAGGTTCA GAGGGACCCC AATTCATTTC 
CCTGGCGGCA GACCTACTAG TGCAGATGCA AAATGCTGGT TTGCAGAAAG GAAGATCTGC 
CAAGGAGGTA TTGACCCTGC CTTGGCCATG ATGGTCTGCT TTGCTTTACT TATAGCCTTG 
CTGTCTATCA ATGGATTTGC TTACTTTATA AGGCGACGTA TAAATAAAAT CCAGTTGATT 
AAAGGACCCA ACAGAATCCT ACTGACTTTG GAAGATGTGA CATTTATTAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGCCG TGCCAGTGTA AGCTTCCAGA TCATCTCAGA AGTTCAAAGT 
GGGAGGTCCC CAAGGCTGTC CTTTTCTTCA GGTAGTCTAA CTCCAGCTAC ATATGAGAAC 
TCCAACATAG CAATCTATGA GGGTGACTGG GTATGGCTGA AAAAGTTCCC TCCTGGAGAT 
TTTGGAGACA TTAAGTCTAT CAAATCGAGT GCAAGTGATG TGTTTGAAAT GATGAAGGAC 
CTGCGGCATG AAAATGTCAA CCCTCTATTG GGTTTCTTCT ATGATTCGGG GATGTTTGCC 
ATCGTGTCAG AATTTTGTTC TCGAAGGAGC CTAGAAGACA TACTTACAAA TGATGATGTG 
AAACTTGATT GGATGTTCAA ATCATCACTA CTGCTTGACC TCATCAAGGG GATGAAATAC 
TTACACCACA GAGAATTTAT TCACGGGAGG CTAAAGTCTC GGAACTGTGT GGTAGATGGG 
CGTTTTGTCC TAAAAGTGAC AGATTATGGC TTTAATGACA TTTTAGAAAT GCTGAGACTC 
TCTGAAGAGG AACCTTCTGA AGAAGAGCTG CTGTGGACGG CCCCTGAACT GTTAAGAGCC 
CCTGGGGGCA TCAGGTTAGG TTCATTTGCA GGAGACGTCT ATAGCTTTGC CATCATCATG 
CAAGAAGTGA TGGTTCGGGG TGCTCCTTTC TGCATGATGG ATCTGCCTGC CAAAGAAATC 
ATAGACAGAC TTAAAATGCC TCCCCCTGTG TACAGACCCG TGGTTTCTCC TGAGTATGCC 
CCTGCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCGTCAGA ACAGCGACCA 
ACTTTTGATG AAATATTTAA CCAGTTTAAA ACTTTCAACA AAGGGAAAAA GACTAATATT 
ATTGATTCTA TGCTTCGGAT GTTGGAGCAG TATTCTAGCA ACTTGGAAGA TTTGATCCGG 
GAACGGACTG AAGAATTGGA AATTGAAAAA CAGAAAACGG AAAAGCTTCT AACACAGATG 
CTACCACTAT CAGTTGCTGA ATCCCTCAAG AAGGGCTGTA CAGTTGAACC TGAGGGATTT 
GACTTGGTCA CCTTGTACTT CAGTGACATT GTGGGCTTCA CCACCATCTC AGCCATGAGT 
GAGCCCATTG AAGTTGTGGA CCTTCTGAAT GACCTGTACA CACTCTTTGA TGCCATCATT 
GGCAGTCATG ATGTCTATAA AGTAGAGACC ATTGGAGATG CCTACATGGT GGCCTCAGGC 
CTTCCCAAGA GGAATGGCAG TAGGCATGCA GCTGAGATTG CAAACATGTC CCTGGATATC 
CTGAGTTCTG TGGGTACCTT TAAGATGCGA CACATGCCAG AGGTGCCAGT CCGAATTCGA 
ATAGGCCTGC ACTCGGGGCC TGTTGTTGCT GGAGTGGTGG GCCTCACCAT GCCCAGATAT 
TGCTTGTTTG GGGACACTGT GAACACAGCT TCTCGAATGG AATCAACAGG GCTACCATAC 
CGAATTCATG TCAGTCTCAG CACAGTAACA ATTCTTCAAA CTCTGAGTGA AGGCTATGAA 
GTAGAGCTTC GTGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAGGAGAC CTTTTGGCTT 
GTTGGAAAAA AGGGTTTCAC CAAACCTCTT CCTGTACCCC CACCAGTGGG CAAAGATGGG 
CAAGTGGGCC ATGGCTTGCA ACCAGCAGAG ATTGCAGCAT TCCAAAGGAG AAAAGCAGAG 
AGGCAGTTGG TGCGAAACAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy2f ( XM_006528841.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528904.1 Mus musculus guanylate cyclase 2f (Gucy2f), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu10777
Clone ID Related Accession (Same CDS sequence)	XM_006528841.3 , XM_006528841.2 , XM_006528841.4 , NM_001007576.2
Accession Version	XM_006528841.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	retinal guanylyl cyclase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528841.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCG GACCCTGGCC TTTTTCTCGC CTTCTGTCCT GGTTTGCCAT TTCCAGCAGA 
CTGTCAGGAC AGCATGGCCT CCCTTCCTCC AAGTTCCTGA GATGTTTGTG CCTCTTGGCC 
CTTTTGCCTC TCCTTCGGTG GGGGCAAGCA CTCCCCTACA AGATCGGGGT CATAGGTCCC 
TGGACATGTG ATCCGTTCTT TTCCAAGGCC CTGCCTGAGG TTGCTGCTGC TTTAGCAATT 
GAGCGAATCA GCCGGGACAA GACATTTGAC AGGAGTTACT CCTTCGAATA CGTGATTCTT 
AACGAAGACT GTCAGACTTC AAAGGCCCTC GCCAGTTTCA TTTCCCACCA GCAGATGGCC 
TCAGGATTTG TTGGGCCTGC CAACCCTGGC TTCTGCGAGG CAGCCTCACT CCTGGGAACC 
AGCTGGGACA AAGGGATTTT CTCTTGGGCT TGTGTGAATC ATGAATTAGA TAACAAACAT 
AGCTTCCCAA CCTTCTCTCG GACACTCCCT TCTCCGATAC GGGTGCTTGT AACCGTCATG 
AAGTATTTCC AGTGGGCACA TGCTGGGGTC ATTTCCTCAG ATGAAGACAT TTGGATGCAT 
ACAGCCAATC GAGTCTCAAG TGCTCTTCGG AGCCAGGGCC TACCTGTAGG GGTCGTCCTG 
ACCTCGGGAC GAGACAGCCA GAGCATTCAG AAAGCTCTCC AGCAGATTCG CCAGGCAGAC 
AGAATTCGCA TAATCATAAT GTGTATGCAT TCAGCCTTGA TTGGGGGAGA GACACAAACA 
CACTTCTTGG AATTGGCTCA TGATCTGAAA ATGACAGATG GGACTTACGT CTTTGTCCCC 
TATGATGTAC TGCTCTACAG TTTACCTTAT AAGCACAGCC CCTACCAGGT CCTGAGGAAC 
AATCCAAAGC TCCGGGAAGC CTATGATGCT GTGTTGACTA TTACAGTGGA GTCCCATGAA 
AAGACCTTCT ACGAAGCCTA TGCAGAAGCA GCAGCCCGCG GAGAAATTCC TGAGAAGCCT 
GACTCAAACC AAGTTTCACC GTTGTTTGGA ACCATCTACA ATTCAATTTA TTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAAAAAAT GGACGGGCTA GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CTATGGATTC AACCAGTTGA TAAAGACAGA CTCAAATGGA 
AATGGGATTT CTGAATATGT AATCCTGGAC ACCAATGGGA AAGAATGGGA ACTTCGTGGC 
ACCTACACTG TGGACATGGA AACTGAACTG CTAAGGTTCA GAGGGACCCC AATTCATTTC 
CCTGGCGGCA GACCTACTAG TGCAGATGCA AAATGCTGGT TTGCAGAAAG GAAGATCTGC 
CAAGGAGGTA TTGACCCTGC CTTGGCCATG ATGGTCTGCT TTGCTTTACT TATAGCCTTG 
CTGTCTATCA ATGGATTTGC TTACTTTATA AGGCGACGTA TAAATAAAAT CCAGTTGATT 
AAAGGACCCA ACAGAATCCT ACTGACTTTG GAAGATGTGA CATTTATTAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGCCG TGCCAGTGTA AGCTTCCAGA TCATCTCAGA AGTTCAAAGT 
GGGAGGTCCC CAAGGCTGTC CTTTTCTTCA GGTAGTCTAA CTCCAGCTAC ATATGAGAAC 
TCCAACATAG CAATCTATGA GGGTGACTGG GTATGGCTGA AAAAGTTCCC TCCTGGAGAT 
TTTGGAGACA TTAAGTCTAT CAAATCGAGT GCAAGTGATG TGTTTGAAAT GATGAAGGAC 
CTGCGGCATG AAAATGTCAA CCCTCTATTG GGTTTCTTCT ATGATTCGGG GATGTTTGCC 
ATCGTGTCAG AATTTTGTTC TCGAAGGAGC CTAGAAGACA TACTTACAAA TGATGATGTG 
AAACTTGATT GGATGTTCAA ATCATCACTA CTGCTTGACC TCATCAAGGG GATGAAATAC 
TTACACCACA GAGAATTTAT TCACGGGAGG CTAAAGTCTC GGAACTGTGT GGTAGATGGG 
CGTTTTGTCC TAAAAGTGAC AGATTATGGC TTTAATGACA TTTTAGAAAT GCTGAGACTC 
TCTGAAGAGG AACCTTCTGA AGAAGAGCTG CTGTGGACGG CCCCTGAACT GTTAAGAGCC 
CCTGGGGGCA TCAGGTTAGG TTCATTTGCA GGAGACGTCT ATAGCTTTGC CATCATCATG 
CAAGAAGTGA TGGTTCGGGG TGCTCCTTTC TGCATGATGG ATCTGCCTGC CAAAGAAATC 
ATAGACAGAC TTAAAATGCC TCCCCCTGTG TACAGACCCG TGGTTTCTCC TGAGTATGCC 
CCTGCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCGTCAGA ACAGCGACCA 
ACTTTTGATG AAATATTTAA CCAGTTTAAA ACTTTCAACA AAGGGAAAAA GACTAATATT 
ATTGATTCTA TGCTTCGGAT GTTGGAGCAG TATTCTAGCA ACTTGGAAGA TTTGATCCGG 
GAACGGACTG AAGAATTGGA AATTGAAAAA CAGAAAACGG AAAAGCTTCT AACACAGATG 
CTACCACTAT CAGTTGCTGA ATCCCTCAAG AAGGGCTGTA CAGTTGAACC TGAGGGATTT 
GACTTGGTCA CCTTGTACTT CAGTGACATT GTGGGCTTCA CCACCATCTC AGCCATGAGT 
GAGCCCATTG AAGTTGTGGA CCTTCTGAAT GACCTGTACA CACTCTTTGA TGCCATCATT 
GGCAGTCATG ATGTCTATAA AGTAGAGACC ATTGGAGATG CCTACATGGT GGCCTCAGGC 
CTTCCCAAGA GGAATGGCAG TAGGCATGCA GCTGAGATTG CAAACATGTC CCTGGATATC 
CTGAGTTCTG TGGGTACCTT TAAGATGCGA CACATGCCAG AGGTGCCAGT CCGAATTCGA 
ATAGGCCTGC ACTCGGGGCC TGTTGTTGCT GGAGTGGTGG GCCTCACCAT GCCCAGATAT 
TGCTTGTTTG GGGACACTGT GAACACAGCT TCTCGAATGG AATCAACAGG GCTACCATAC 
CGAATTCATG TCAGTCTCAG CACAGTAACA ATTCTTCAAA CTCTGAGTGA AGGCTATGAA 
GTAGAGCTTC GTGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAGGAGAC CTTTTGGCTT 
GTTGGAAAAA AGGGTTTCAC CAAACCTCTT CCTGTACCCC CACCAGTGGG CAAAGATGGG 
CAAGTGGGCC ATGGCTTGCA ACCAGCAGAG ATTGCAGCAT TCCAAAGGAG AAAAGCAGAG 
AGGCAGTTGG TGCGAAACAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528904.1
CDS	395..3721
Translation	MFLGPWPFSRLLSWFAISSRLSGQHGLPSSKFLRCLCLLALLPLLRWGQALPYKIGVIGPWTCDPFFSKALPEVAAALAIERISRDKTFDRSYSFEYVILNEDCQTSKALASFISHQQMASGFVGPANPGFCEAASLLGTSWDKGIFSWACVNHELDNKHSFPTFSRTLPSPIRVLVTVMKYFQWAHAGVISSDEDIWMHTANRVSSALRSQGLPVGVVLTSGRDSQSIQKALQQIRQADRIRIIIMCMHSALIGGETQTHFLELAHDLKMTDGTYVFVPYDVLLYSLPYKHSPYQVLRNNPKLREAYDAVLTITVESHEKTFYEAYAEAAARGEIPEKPDSNQVSPLFGTIYNSIYFIAQAMNNAMKKNGRASAASLVQHSRNMQFYGFNQLIKTDSNGNGISEYVILDTNGKEWELRGTYTVDMETELLRFRGTPIHFPGGRPTSADAKCWFAERKICQGGIDPALAMMVCFALLIALLSINGFAYFIRRRINKIQLIKGPNRILLTLEDVTFINPHFGSKRGSRASVSFQIISEVQSGRSPRLSFSSGSLTPATYENSNIAIYEGDWVWLKKFPPGDFGDIKSIKSSASDVFEMMKDLRHENVNPLLGFFYDSGMFAIVSEFCSRRSLEDILTNDDVKLDWMFKSSLLLDLIKGMKYLHHREFIHGRLKSRNCVVDGRFVLKVTDYGFNDILEMLRLSEEEPSEEELLWTAPELLRAPGGIRLGSFAGDVYSFAIIMQEVMVRGAPFCMMDLPAKEIIDRLKMPPPVYRPVVSPEYAPAECLQLMKQCWAEASEQRPTFDEIFNQFKTFNKGKKTNIIDSMLRMLEQYSSNLEDLIRERTEELEIEKQKTEKLLTQMLPLSVAESLKKGCTVEPEGFDLVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPKRNGSRHAAEIANMSLDILSSVGTFKMRHMPEVPVRIRIGLHSGPVVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSLSTVTILQTLSEGYEVELRGRTELKGKGTEETFWLVGKKGFTKPLPVPPPVGKDGQVGHGLQPAEIAAFQRRKAERQLVRNKP

Target ORF information:

RefSeq Version	XM_006528841.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanylate cyclase 2f (Gucy2f), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528841.4

ORF Insert Sequence:

ATGTTCCTCG GACCCTGGCC TTTTTCTCGC CTTCTGTCCT GGTTTGCCAT TTCCAGCAGA 
CTGTCAGGAC AGCATGGCCT CCCTTCCTCC AAGTTCCTGA GATGTTTGTG CCTCTTGGCC 
CTTTTGCCTC TCCTTCGGTG GGGGCAAGCA CTCCCCTACA AGATCGGGGT CATAGGTCCC 
TGGACATGTG ATCCGTTCTT TTCCAAGGCC CTGCCTGAGG TTGCTGCTGC TTTAGCAATT 
GAGCGAATCA GCCGGGACAA GACATTTGAC AGGAGTTACT CCTTCGAATA CGTGATTCTT 
AACGAAGACT GTCAGACTTC AAAGGCCCTC GCCAGTTTCA TTTCCCACCA GCAGATGGCC 
TCAGGATTTG TTGGGCCTGC CAACCCTGGC TTCTGCGAGG CAGCCTCACT CCTGGGAACC 
AGCTGGGACA AAGGGATTTT CTCTTGGGCT TGTGTGAATC ATGAATTAGA TAACAAACAT 
AGCTTCCCAA CCTTCTCTCG GACACTCCCT TCTCCGATAC GGGTGCTTGT AACCGTCATG 
AAGTATTTCC AGTGGGCACA TGCTGGGGTC ATTTCCTCAG ATGAAGACAT TTGGATGCAT 
ACAGCCAATC GAGTCTCAAG TGCTCTTCGG AGCCAGGGCC TACCTGTAGG GGTCGTCCTG 
ACCTCGGGAC GAGACAGCCA GAGCATTCAG AAAGCTCTCC AGCAGATTCG CCAGGCAGAC 
AGAATTCGCA TAATCATAAT GTGTATGCAT TCAGCCTTGA TTGGGGGAGA GACACAAACA 
CACTTCTTGG AATTGGCTCA TGATCTGAAA ATGACAGATG GGACTTACGT CTTTGTCCCC 
TATGATGTAC TGCTCTACAG TTTACCTTAT AAGCACAGCC CCTACCAGGT CCTGAGGAAC 
AATCCAAAGC TCCGGGAAGC CTATGATGCT GTGTTGACTA TTACAGTGGA GTCCCATGAA 
AAGACCTTCT ACGAAGCCTA TGCAGAAGCA GCAGCCCGCG GAGAAATTCC TGAGAAGCCT 
GACTCAAACC AAGTTTCACC GTTGTTTGGA ACCATCTACA ATTCAATTTA TTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAAAAAAT GGACGGGCTA GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CTATGGATTC AACCAGTTGA TAAAGACAGA CTCAAATGGA 
AATGGGATTT CTGAATATGT AATCCTGGAC ACCAATGGGA AAGAATGGGA ACTTCGTGGC 
ACCTACACTG TGGACATGGA AACTGAACTG CTAAGGTTCA GAGGGACCCC AATTCATTTC 
CCTGGCGGCA GACCTACTAG TGCAGATGCA AAATGCTGGT TTGCAGAAAG GAAGATCTGC 
CAAGGAGGTA TTGACCCTGC CTTGGCCATG ATGGTCTGCT TTGCTTTACT TATAGCCTTG 
CTGTCTATCA ATGGATTTGC TTACTTTATA AGGCGACGTA TAAATAAAAT CCAGTTGATT 
AAAGGACCCA ACAGAATCCT ACTGACTTTG GAAGATGTGA CATTTATTAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGCCG TGCCAGTGTA AGCTTCCAGA TCATCTCAGA AGTTCAAAGT 
GGGAGGTCCC CAAGGCTGTC CTTTTCTTCA GGTAGTCTAA CTCCAGCTAC ATATGAGAAC 
TCCAACATAG CAATCTATGA GGGTGACTGG GTATGGCTGA AAAAGTTCCC TCCTGGAGAT 
TTTGGAGACA TTAAGTCTAT CAAATCGAGT GCAAGTGATG TGTTTGAAAT GATGAAGGAC 
CTGCGGCATG AAAATGTCAA CCCTCTATTG GGTTTCTTCT ATGATTCGGG GATGTTTGCC 
ATCGTGTCAG AATTTTGTTC TCGAAGGAGC CTAGAAGACA TACTTACAAA TGATGATGTG 
AAACTTGATT GGATGTTCAA ATCATCACTA CTGCTTGACC TCATCAAGGG GATGAAATAC 
TTACACCACA GAGAATTTAT TCACGGGAGG CTAAAGTCTC GGAACTGTGT GGTAGATGGG 
CGTTTTGTCC TAAAAGTGAC AGATTATGGC TTTAATGACA TTTTAGAAAT GCTGAGACTC 
TCTGAAGAGG AACCTTCTGA AGAAGAGCTG CTGTGGACGG CCCCTGAACT GTTAAGAGCC 
CCTGGGGGCA TCAGGTTAGG TTCATTTGCA GGAGACGTCT ATAGCTTTGC CATCATCATG 
CAAGAAGTGA TGGTTCGGGG TGCTCCTTTC TGCATGATGG ATCTGCCTGC CAAAGAAATC 
ATAGACAGAC TTAAAATGCC TCCCCCTGTG TACAGACCCG TGGTTTCTCC TGAGTATGCC 
CCTGCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCGTCAGA ACAGCGACCA 
ACTTTTGATG AAATATTTAA CCAGTTTAAA ACTTTCAACA AAGGGAAAAA GACTAATATT 
ATTGATTCTA TGCTTCGGAT GTTGGAGCAG TATTCTAGCA ACTTGGAAGA TTTGATCCGG 
GAACGGACTG AAGAATTGGA AATTGAAAAA CAGAAAACGG AAAAGCTTCT AACACAGATG 
CTACCACTAT CAGTTGCTGA ATCCCTCAAG AAGGGCTGTA CAGTTGAACC TGAGGGATTT 
GACTTGGTCA CCTTGTACTT CAGTGACATT GTGGGCTTCA CCACCATCTC AGCCATGAGT 
GAGCCCATTG AAGTTGTGGA CCTTCTGAAT GACCTGTACA CACTCTTTGA TGCCATCATT 
GGCAGTCATG ATGTCTATAA AGTAGAGACC ATTGGAGATG CCTACATGGT GGCCTCAGGC 
CTTCCCAAGA GGAATGGCAG TAGGCATGCA GCTGAGATTG CAAACATGTC CCTGGATATC 
CTGAGTTCTG TGGGTACCTT TAAGATGCGA CACATGCCAG AGGTGCCAGT CCGAATTCGA 
ATAGGCCTGC ACTCGGGGCC TGTTGTTGCT GGAGTGGTGG GCCTCACCAT GCCCAGATAT 
TGCTTGTTTG GGGACACTGT GAACACAGCT TCTCGAATGG AATCAACAGG GCTACCATAC 
CGAATTCATG TCAGTCTCAG CACAGTAACA ATTCTTCAAA CTCTGAGTGA AGGCTATGAA 
GTAGAGCTTC GTGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAGGAGAC CTTTTGGCTT 
GTTGGAAAAA AGGGTTTCAC CAAACCTCTT CCTGTACCCC CACCAGTGGG CAAAGATGGG 
CAAGTGGGCC ATGGCTTGCA ACCAGCAGAG ATTGCAGCAT TCCAAAGGAG AAAAGCAGAG 
AGGCAGTTGG TGCGAAACAA GCCATAA