Ythdc2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ythdc2
Entrez Gene ID	240255
Full Name	YTH domain containing 2
Synonyms	3010002F02Rik,BC037178
General protein information	Preferred Names YTH domain containing 2 Names probable ATP-dependent RNA helicase YTHDC2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001163013.1	NP_001156485.1	3'-5' RNA helicase YTHDC2
XM_030250454.1	XP_030106314.1	3'-5' RNA helicase YTHDC2 isoform X1
XM_006525915.2	XP_006525978.1	probable ATP-dependent RNA helicase YTHDC2 isoform X1
XM_006525915.3	XP_006525978.1	3'-5' RNA helicase YTHDC2 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ythdc2	NP_001156485.1
Arabidopsis thaliana (thale cress)	NIH	NP_172152.1
Homo sapiens (human)	YTHDC2	NP_073739.3
Bos taurus (cattle)	YTHDC2	NP_001179740.1
Rattus norvegicus (Norway rat)	Ythdc2	XP_003749024.1
Gallus gallus (chicken)	YTHDC2	XP_413970.2
Pan troglodytes (chimpanzee)	YTHDC2	XP_517881.2
Canis lupus familiaris (dog)	LOC474641	XP_005626411.1
Arabidopsis thaliana (thale cress)	HVT1	NP_850154.2
Xenopus tropicalis (tropical clawed frog)	ythdc2	XP_002936459.2
Oryza sativa (rice)	Os01g0767700	NP_001044362.1
	YTHDC2	XP_002804526.1
Danio rerio (zebrafish)	LOC567114	XP_695496.6
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007953	XP_555531.3

Gene Ontology
Bibliography

Related articles in PubMed

Jain D, Puno MR, Meydan C, Lailler N, Mason CE, Lima CD, Anderson KV, Keeney S
eLife7(2018 Jan)

The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline.
Bailey AS, Batista PJ, Gold RS, Chen YG, de Rooij DG, Chang HY, Fuller MT
eLife6(2017 Oct)

Regulation of m
Wojtas MN, Pandey RR, Mendel M, Homolka D, Sachidanandam R, Pillai RS
Molecular cell68(2)374-387.e12(2017 Oct)

Ythdc2 is an N
Hsu PJ, Zhu Y, Ma H, Guo Y, Shi X, Liu Y, Qi M, Lu Z, Shi H, Wang J, Cheng Y, Luo G, Dai Q, Liu M, Guo X, Sha J, Shen B, He C
Cell research27(9)1115-1127(2017 Sep)

Meioc maintains an extended meiotic prophase I in mice.
Soh YQS, Mikedis MM, Kojima M, Godfrey AK, de Rooij DG, Page DC
PLoS genetics13(4)e1006704(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

studies reveal a role for YTHDC2 in modulating the levels of m(6)A-modified germline transcripts to maintain a gene expression program that is conducive for progression through meiosis.
Title: Regulation of m

We conclude that when the meiotic chromosomal program is initiated, Meioc is simultaneously induced so as to extend meiotic prophase. Specifically, MEIOC, together with YTHDC2, promotes a meiotic (as opposed to mitotic) cell cycle program via post-transcriptional control of their target transcripts.
Title: Meioc maintains an extended meiotic prophase I in mice.

The following Ythdc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ythdc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu54571	XM_006525915.3 Latest version!	Mus musculus YTH domain containing 2 (Ythdc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu87394	XM_030250454.1 Latest version!	Mus musculus YTH domain containing 2 (Ythdc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu08687	NM_001163013.1 Latest version!	Mus musculus YTH domain containing 2 (Ythdc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OMu54571	XM_006525915.2	Mus musculus YTH domain containing 2 (Ythdc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ythdc2 ( XM_006525915.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525978.1 Mus musculus YTH domain containing 2 (Ythdc2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu54571
Clone ID Related Accession (Same CDS sequence)	XM_006525915.2 , XM_006525915.3
Accession Version	XM_006525915.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase YTHDC2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006525915.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAG ATTGGGCTAA GCACTTTGGA CAGACTGAAA TTGTGGATCT TCTAGAATCT 
TACAGTGCTT CATTGGAATT TGGAAATCTA GATGAAAGTT CCTTGGTTCA GACAAATGGG 
AATGACCTCA GTGCAGAAGA CAGAGAGCTG TTGAAAGCTT ACCATCATAG TTTTGATGAT 
GAGAAAGTAG ACTTGGATTT GATCATGCAT CTCCTATATA ATATCTGCCA TAGCTGTGAT 
GCTGGTGCAA TATTAATTTT TCTACCTGGA TATGATGAAA TTGTTGGTTT GAGGGATCGT 
ATCCTCTTTG ATGACAAGCG GTTTGCTGAC AATACACATA GGTATCAAGT CTTTATGCTT 
CATTCAAATA TGCAGACATC TGATCAAAAG AAGGTATTAA AAAATCCACC TGCAGGTGTC 
CGAAAAATAA TCCTTTCCAC CAATATTGCT GAGACGAGCA TCACTGTCAA TGATGTTGTG 
TTTGTTATTG ATTCTGGTAA GGTGAAAGAG AAATCCTTTG ATGCACTGAA TTTTGTTACA 
ATGTTAAAAA TGGTATGGAT TTCCAAAGCT AGTGCAATAC AGCGCAAAGG CAGAGCGGGA 
CGGTGTAGAC CTGGAATTTG TTTCCGTCTG TTTAGTAGAC TTCGATTTCA GAACATGCTG 
GAATTTCAGA CGCCAGAACT CTTGAGAATG CCATTGCAGG AACTTTGTTT ACATACCAAG 
CTGTTAGCCC CAGTTAACTG CACCATTGCC GACTTCCTTA TGAAAGCCCC TGAACCGCCT 
CCAGCTTTAA TTGTGAGAAA TGCTGTGCAG ATGCTTAAGA CAATAGATGC AATGGATGCA 
TGGGAAGATC TGACTGAACT TGGGTATCAT TTGGCTGATT TGCCAGTAGA ACCGCATCTT 
GGGAAAATGG TCCTGTGTGC AGTGGTTTTG AAGTGTCTGG ACCCCATCCT TACAATTGCC 
TGCACGCTCG CTTACCGTGA CCCTTTTGTC CTGCCTACCC AGGCCTCTCA GAAACGGGCA 
GCTATGCTTT GTAGGAAACG CTTCACTGCA GGGACTTTCA GTGACCACAT GGCACTTCTG 
AGAGCATTTC AGGCATGGCA GAAAGCACGA AGTGATGGGT GGGAGCGAGC CTTTTGTGAA 
AAGAATTTTC TTTCACAAGC TACTATGGAA ATCATTATAG GCATGAGAAC ACAGTTGCTT 
GGTCAGCTTA GAGCATCAGG TTTTGTTAGA GCACGAGGTG GTGGTGACAT TCGAGATGTT 
AATACAAACT CCGAGAACTG GGCTGTTGTT AAAGCTGCAT TGGTAGCAGG CATGTATCCT 
AATTTAGTCC ACGTGGACAG AGAGAATGTA ATATTGACAG GGCCAAAGGA AAAGAAAGTA 
CGCTTTCATC CCACTTCAGT TCTCAGTCAA CCTCAATACA AAAAGATTCC TCCCGCCAAT 
GGTCAGGCTG CAGCGATTCA GGCATTGCCC ACAGATTGGC TTATTTATGA TGAAATGACC 
AGAGCCCATC GAATTGCTAA TATCAGATGT TGTTCAGCAG TGACACCTGT TACTGTCCTG 
GTGTTCTGTG GACCAGCAAG ACTGGCAAGT AACGCTCTTC AGGAACCTTC ATCCTTTAGA 
GCGGATGGTA TTCCCAATGA CAGCAGTGAC AGTGAGATGG AAGACAGAAC TACTGCCAAT 
TTGGCTGCTT TGAAGCTTGA TGAATGGCTT AACTTCAAAC TAGAGCCAGA GGCTGCCAGT 
TTATTGCTAC AGCTCAGACA GAAGTGGCAT AGTTTGTTTT TACGCCGAAT GAGAGCGCCA 
TCTAAACCTT GGTCTCAAGT CGATGAAGCT ACCATACGAG CAATTATAGC TGTTTTAAGC 
ACTGAAGAAC AGTCTGCAGG TTTACAGCAG CCATCTGGAA TTGGCCAGCG GCCAAGACCC 
ATGTCTTCAG AAGAACTTCC TTTGGCTTCA TCTTGGAGAT CCAATAATAG TAGGAAAAGT 
ACAGCTGACA CTGAGTTTGC GGATGGATCT ACTACTGGAG AAAGAGTACT GATGAAATCT 
CCATCTCCAG CCTTACATCC ACCTCAGAAG TACAAAGATA GAGGAATTTT ACATCCTAAG 
CGGAGCACCG ATGACCGGTC CGATCAATCA TCTGTGAAAT CCACAGACAG CAGCAGTTAC 
CCAAGTCCTT GTGCCAGCCC CTCTCCTCCA TCCTCAGGGA AGGGATCAAA ATCTCCTTCA 
CCAAGACCAA ACATGCCTAT TCGTTACTTC ATAATGAAGA GTAGCAATCT GAGAAACCTT 
GAAATTTCTC AACAGAAGGG TATCTGGTCT ACAACCCCTA GTAATGAGCG GAAGCTAAAT 
CGAGCGTTTT GGGAGAGCAG CATGGTTTAC TTGGTATTTT CTGTTCAAGG ATCTGGACAT 
TTCCAGGGAT TTTCTAGGAT GTCTTCTGAG ATAGGAAGGG AGAAGAGCCA GGACTGGGGG 
TCAGCTGGAC TAGGTGGAGT ATTTAAAGTG GAGTGGATAC GCAAAGAAAG CCTTCCCTTT 
CAGTTTGCAC ACCATTTACT CAATCCTTGG AACGACAACA AGAAAGTACA GATAAGCAGA 
GATGGGCAGG AGCTAGAACC TCAGGTTGGG GAACAGTTGC TCCAGTTATG GGAACGCCTT 
CCATTGGGAG AAAAAACAAC TTCTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525978.1
CDS	89..2758
Translation	MALDWAKHFGQTEIVDLLESYSASLEFGNLDESSLVQTNGNDLSAEDRELLKAYHHSFDDEKVDLDLIMHLLYNICHSCDAGAILIFLPGYDEIVGLRDRILFDDKRFADNTHRYQVFMLHSNMQTSDQKKVLKNPPAGVRKIILSTNIAETSITVNDVVFVIDSGKVKEKSFDALNFVTMLKMVWISKASAIQRKGRAGRCRPGICFRLFSRLRFQNMLEFQTPELLRMPLQELCLHTKLLAPVNCTIADFLMKAPEPPPALIVRNAVQMLKTIDAMDAWEDLTELGYHLADLPVEPHLGKMVLCAVVLKCLDPILTIACTLAYRDPFVLPTQASQKRAAMLCRKRFTAGTFSDHMALLRAFQAWQKARSDGWERAFCEKNFLSQATMEIIIGMRTQLLGQLRASGFVRARGGGDIRDVNTNSENWAVVKAALVAGMYPNLVHVDRENVILTGPKEKKVRFHPTSVLSQPQYKKIPPANGQAAAIQALPTDWLIYDEMTRAHRIANIRCCSAVTPVTVLVFCGPARLASNALQEPSSFRADGIPNDSSDSEMEDRTTANLAALKLDEWLNFKLEPEAASLLLQLRQKWHSLFLRRMRAPSKPWSQVDEATIRAIIAVLSTEEQSAGLQQPSGIGQRPRPMSSEELPLASSWRSNNSRKSTADTEFADGSTTGERVLMKSPSPALHPPQKYKDRGILHPKRSTDDRSDQSSVKSTDSSSYPSPCASPSPPSSGKGSKSPSPRPNMPIRYFIMKSSNLRNLEISQQKGIWSTTPSNERKLNRAFWESSMVYLVFSVQGSGHFQGFSRMSSEIGREKSQDWGSAGLGGVFKVEWIRKESLPFQFAHHLLNPWNDNKKVQISRDGQELEPQVGEQLLQLWERLPLGEKTTSD

Target ORF information:

RefSeq Version	XM_006525915.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus YTH domain containing 2 (Ythdc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525915.2

ORF Insert Sequence:

ATGGCTTTAG ATTGGGCTAA GCACTTTGGA CAGACTGAAA TTGTGGATCT TCTAGAATCT 
TACAGTGCTT CATTGGAATT TGGAAATCTA GATGAAAGTT CCTTGGTTCA GACAAATGGG 
AATGACCTCA GTGCAGAAGA CAGAGAGCTG TTGAAAGCTT ACCATCATAG TTTTGATGAT 
GAGAAAGTAG ACTTGGATTT GATCATGCAT CTCCTATATA ATATCTGCCA TAGCTGTGAT 
GCTGGTGCAA TATTAATTTT TCTACCTGGA TATGATGAAA TTGTTGGTTT GAGGGATCGT 
ATCCTCTTTG ATGACAAGCG GTTTGCTGAC AATACACATA GGTATCAAGT CTTTATGCTT 
CATTCAAATA TGCAGACATC TGATCAAAAG AAGGTATTAA AAAATCCACC TGCAGGTGTC 
CGAAAAATAA TCCTTTCCAC CAATATTGCT GAGACGAGCA TCACTGTCAA TGATGTTGTG 
TTTGTTATTG ATTCTGGTAA GGTGAAAGAG AAATCCTTTG ATGCACTGAA TTTTGTTACA 
ATGTTAAAAA TGGTATGGAT TTCCAAAGCT AGTGCAATAC AGCGCAAAGG CAGAGCGGGA 
CGGTGTAGAC CTGGAATTTG TTTCCGTCTG TTTAGTAGAC TTCGATTTCA GAACATGCTG 
GAATTTCAGA CGCCAGAACT CTTGAGAATG CCATTGCAGG AACTTTGTTT ACATACCAAG 
CTGTTAGCCC CAGTTAACTG CACCATTGCC GACTTCCTTA TGAAAGCCCC TGAACCGCCT 
CCAGCTTTAA TTGTGAGAAA TGCTGTGCAG ATGCTTAAGA CAATAGATGC AATGGATGCA 
TGGGAAGATC TGACTGAACT TGGGTATCAT TTGGCTGATT TGCCAGTAGA ACCGCATCTT 
GGGAAAATGG TCCTGTGTGC AGTGGTTTTG AAGTGTCTGG ACCCCATCCT TACAATTGCC 
TGCACGCTCG CTTACCGTGA CCCTTTTGTC CTGCCTACCC AGGCCTCTCA GAAACGGGCA 
GCTATGCTTT GTAGGAAACG CTTCACTGCA GGGACTTTCA GTGACCACAT GGCACTTCTG 
AGAGCATTTC AGGCATGGCA GAAAGCACGA AGTGATGGGT GGGAGCGAGC CTTTTGTGAA 
AAGAATTTTC TTTCACAAGC TACTATGGAA ATCATTATAG GCATGAGAAC ACAGTTGCTT 
GGTCAGCTTA GAGCATCAGG TTTTGTTAGA GCACGAGGTG GTGGTGACAT TCGAGATGTT 
AATACAAACT CCGAGAACTG GGCTGTTGTT AAAGCTGCAT TGGTAGCAGG CATGTATCCT 
AATTTAGTCC ACGTGGACAG AGAGAATGTA ATATTGACAG GGCCAAAGGA AAAGAAAGTA 
CGCTTTCATC CCACTTCAGT TCTCAGTCAA CCTCAATACA AAAAGATTCC TCCCGCCAAT 
GGTCAGGCTG CAGCGATTCA GGCATTGCCC ACAGATTGGC TTATTTATGA TGAAATGACC 
AGAGCCCATC GAATTGCTAA TATCAGATGT TGTTCAGCAG TGACACCTGT TACTGTCCTG 
GTGTTCTGTG GACCAGCAAG ACTGGCAAGT AACGCTCTTC AGGAACCTTC ATCCTTTAGA 
GCGGATGGTA TTCCCAATGA CAGCAGTGAC AGTGAGATGG AAGACAGAAC TACTGCCAAT 
TTGGCTGCTT TGAAGCTTGA TGAATGGCTT AACTTCAAAC TAGAGCCAGA GGCTGCCAGT 
TTATTGCTAC AGCTCAGACA GAAGTGGCAT AGTTTGTTTT TACGCCGAAT GAGAGCGCCA 
TCTAAACCTT GGTCTCAAGT CGATGAAGCT ACCATACGAG CAATTATAGC TGTTTTAAGC 
ACTGAAGAAC AGTCTGCAGG TTTACAGCAG CCATCTGGAA TTGGCCAGCG GCCAAGACCC 
ATGTCTTCAG AAGAACTTCC TTTGGCTTCA TCTTGGAGAT CCAATAATAG TAGGAAAAGT 
ACAGCTGACA CTGAGTTTGC GGATGGATCT ACTACTGGAG AAAGAGTACT GATGAAATCT 
CCATCTCCAG CCTTACATCC ACCTCAGAAG TACAAAGATA GAGGAATTTT ACATCCTAAG 
CGGAGCACCG ATGACCGGTC CGATCAATCA TCTGTGAAAT CCACAGACAG CAGCAGTTAC 
CCAAGTCCTT GTGCCAGCCC CTCTCCTCCA TCCTCAGGGA AGGGATCAAA ATCTCCTTCA 
CCAAGACCAA ACATGCCTAT TCGTTACTTC ATAATGAAGA GTAGCAATCT GAGAAACCTT 
GAAATTTCTC AACAGAAGGG TATCTGGTCT ACAACCCCTA GTAATGAGCG GAAGCTAAAT 
CGAGCGTTTT GGGAGAGCAG CATGGTTTAC TTGGTATTTT CTGTTCAAGG ATCTGGACAT 
TTCCAGGGAT TTTCTAGGAT GTCTTCTGAG ATAGGAAGGG AGAAGAGCCA GGACTGGGGG 
TCAGCTGGAC TAGGTGGAGT ATTTAAAGTG GAGTGGATAC GCAAAGAAAG CCTTCCCTTT 
CAGTTTGCAC ACCATTTACT CAATCCTTGG AACGACAACA AGAAAGTACA GATAAGCAGA 
GATGGGCAGG AGCTAGAACC TCAGGTTGGG GAACAGTTGC TCCAGTTATG GGAACGCCTT 
CCATTGGGAG AAAAAACAAC TTCTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ythdc2 ( XM_006525915.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525978.1 Mus musculus YTH domain containing 2 (Ythdc2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu54571
Clone ID Related Accession (Same CDS sequence)	XM_006525915.2 , XM_006525915.3
Accession Version	XM_006525915.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	3'-5' RNA helicase YTHDC2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525915.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAG ATTGGGCTAA GCACTTTGGA CAGACTGAAA TTGTGGATCT TCTAGAATCT 
TACAGTGCTT CATTGGAATT TGGAAATCTA GATGAAAGTT CCTTGGTTCA GACAAATGGG 
AATGACCTCA GTGCAGAAGA CAGAGAGCTG TTGAAAGCTT ACCATCATAG TTTTGATGAT 
GAGAAAGTAG ACTTGGATTT GATCATGCAT CTCCTATATA ATATCTGCCA TAGCTGTGAT 
GCTGGTGCAA TATTAATTTT TCTACCTGGA TATGATGAAA TTGTTGGTTT GAGGGATCGT 
ATCCTCTTTG ATGACAAGCG GTTTGCTGAC AATACACATA GGTATCAAGT CTTTATGCTT 
CATTCAAATA TGCAGACATC TGATCAAAAG AAGGTATTAA AAAATCCACC TGCAGGTGTC 
CGAAAAATAA TCCTTTCCAC CAATATTGCT GAGACGAGCA TCACTGTCAA TGATGTTGTG 
TTTGTTATTG ATTCTGGTAA GGTGAAAGAG AAATCCTTTG ATGCACTGAA TTTTGTTACA 
ATGTTAAAAA TGGTATGGAT TTCCAAAGCT AGTGCAATAC AGCGCAAAGG CAGAGCGGGA 
CGGTGTAGAC CTGGAATTTG TTTCCGTCTG TTTAGTAGAC TTCGATTTCA GAACATGCTG 
GAATTTCAGA CGCCAGAACT CTTGAGAATG CCATTGCAGG AACTTTGTTT ACATACCAAG 
CTGTTAGCCC CAGTTAACTG CACCATTGCC GACTTCCTTA TGAAAGCCCC TGAACCGCCT 
CCAGCTTTAA TTGTGAGAAA TGCTGTGCAG ATGCTTAAGA CAATAGATGC AATGGATGCA 
TGGGAAGATC TGACTGAACT TGGGTATCAT TTGGCTGATT TGCCAGTAGA ACCGCATCTT 
GGGAAAATGG TCCTGTGTGC AGTGGTTTTG AAGTGTCTGG ACCCCATCCT TACAATTGCC 
TGCACGCTCG CTTACCGTGA CCCTTTTGTC CTGCCTACCC AGGCCTCTCA GAAACGGGCA 
GCTATGCTTT GTAGGAAACG CTTCACTGCA GGGACTTTCA GTGACCACAT GGCACTTCTG 
AGAGCATTTC AGGCATGGCA GAAAGCACGA AGTGATGGGT GGGAGCGAGC CTTTTGTGAA 
AAGAATTTTC TTTCACAAGC TACTATGGAA ATCATTATAG GCATGAGAAC ACAGTTGCTT 
GGTCAGCTTA GAGCATCAGG TTTTGTTAGA GCACGAGGTG GTGGTGACAT TCGAGATGTT 
AATACAAACT CCGAGAACTG GGCTGTTGTT AAAGCTGCAT TGGTAGCAGG CATGTATCCT 
AATTTAGTCC ACGTGGACAG AGAGAATGTA ATATTGACAG GGCCAAAGGA AAAGAAAGTA 
CGCTTTCATC CCACTTCAGT TCTCAGTCAA CCTCAATACA AAAAGATTCC TCCCGCCAAT 
GGTCAGGCTG CAGCGATTCA GGCATTGCCC ACAGATTGGC TTATTTATGA TGAAATGACC 
AGAGCCCATC GAATTGCTAA TATCAGATGT TGTTCAGCAG TGACACCTGT TACTGTCCTG 
GTGTTCTGTG GACCAGCAAG ACTGGCAAGT AACGCTCTTC AGGAACCTTC ATCCTTTAGA 
GCGGATGGTA TTCCCAATGA CAGCAGTGAC AGTGAGATGG AAGACAGAAC TACTGCCAAT 
TTGGCTGCTT TGAAGCTTGA TGAATGGCTT AACTTCAAAC TAGAGCCAGA GGCTGCCAGT 
TTATTGCTAC AGCTCAGACA GAAGTGGCAT AGTTTGTTTT TACGCCGAAT GAGAGCGCCA 
TCTAAACCTT GGTCTCAAGT CGATGAAGCT ACCATACGAG CAATTATAGC TGTTTTAAGC 
ACTGAAGAAC AGTCTGCAGG TTTACAGCAG CCATCTGGAA TTGGCCAGCG GCCAAGACCC 
ATGTCTTCAG AAGAACTTCC TTTGGCTTCA TCTTGGAGAT CCAATAATAG TAGGAAAAGT 
ACAGCTGACA CTGAGTTTGC GGATGGATCT ACTACTGGAG AAAGAGTACT GATGAAATCT 
CCATCTCCAG CCTTACATCC ACCTCAGAAG TACAAAGATA GAGGAATTTT ACATCCTAAG 
CGGAGCACCG ATGACCGGTC CGATCAATCA TCTGTGAAAT CCACAGACAG CAGCAGTTAC 
CCAAGTCCTT GTGCCAGCCC CTCTCCTCCA TCCTCAGGGA AGGGATCAAA ATCTCCTTCA 
CCAAGACCAA ACATGCCTAT TCGTTACTTC ATAATGAAGA GTAGCAATCT GAGAAACCTT 
GAAATTTCTC AACAGAAGGG TATCTGGTCT ACAACCCCTA GTAATGAGCG GAAGCTAAAT 
CGAGCGTTTT GGGAGAGCAG CATGGTTTAC TTGGTATTTT CTGTTCAAGG ATCTGGACAT 
TTCCAGGGAT TTTCTAGGAT GTCTTCTGAG ATAGGAAGGG AGAAGAGCCA GGACTGGGGG 
TCAGCTGGAC TAGGTGGAGT ATTTAAAGTG GAGTGGATAC GCAAAGAAAG CCTTCCCTTT 
CAGTTTGCAC ACCATTTACT CAATCCTTGG AACGACAACA AGAAAGTACA GATAAGCAGA 
GATGGGCAGG AGCTAGAACC TCAGGTTGGG GAACAGTTGC TCCAGTTATG GGAACGCCTT 
CCATTGGGAG AAAAAACAAC TTCTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525978.1
CDS	77..2746
Translation	MALDWAKHFGQTEIVDLLESYSASLEFGNLDESSLVQTNGNDLSAEDRELLKAYHHSFDDEKVDLDLIMHLLYNICHSCDAGAILIFLPGYDEIVGLRDRILFDDKRFADNTHRYQVFMLHSNMQTSDQKKVLKNPPAGVRKIILSTNIAETSITVNDVVFVIDSGKVKEKSFDALNFVTMLKMVWISKASAIQRKGRAGRCRPGICFRLFSRLRFQNMLEFQTPELLRMPLQELCLHTKLLAPVNCTIADFLMKAPEPPPALIVRNAVQMLKTIDAMDAWEDLTELGYHLADLPVEPHLGKMVLCAVVLKCLDPILTIACTLAYRDPFVLPTQASQKRAAMLCRKRFTAGTFSDHMALLRAFQAWQKARSDGWERAFCEKNFLSQATMEIIIGMRTQLLGQLRASGFVRARGGGDIRDVNTNSENWAVVKAALVAGMYPNLVHVDRENVILTGPKEKKVRFHPTSVLSQPQYKKIPPANGQAAAIQALPTDWLIYDEMTRAHRIANIRCCSAVTPVTVLVFCGPARLASNALQEPSSFRADGIPNDSSDSEMEDRTTANLAALKLDEWLNFKLEPEAASLLLQLRQKWHSLFLRRMRAPSKPWSQVDEATIRAIIAVLSTEEQSAGLQQPSGIGQRPRPMSSEELPLASSWRSNNSRKSTADTEFADGSTTGERVLMKSPSPALHPPQKYKDRGILHPKRSTDDRSDQSSVKSTDSSSYPSPCASPSPPSSGKGSKSPSPRPNMPIRYFIMKSSNLRNLEISQQKGIWSTTPSNERKLNRAFWESSMVYLVFSVQGSGHFQGFSRMSSEIGREKSQDWGSAGLGGVFKVEWIRKESLPFQFAHHLLNPWNDNKKVQISRDGQELEPQVGEQLLQLWERLPLGEKTTSD

Target ORF information:

RefSeq Version	XM_006525915.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus YTH domain containing 2 (Ythdc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525915.3

ORF Insert Sequence:

ATGGCTTTAG ATTGGGCTAA GCACTTTGGA CAGACTGAAA TTGTGGATCT TCTAGAATCT 
TACAGTGCTT CATTGGAATT TGGAAATCTA GATGAAAGTT CCTTGGTTCA GACAAATGGG 
AATGACCTCA GTGCAGAAGA CAGAGAGCTG TTGAAAGCTT ACCATCATAG TTTTGATGAT 
GAGAAAGTAG ACTTGGATTT GATCATGCAT CTCCTATATA ATATCTGCCA TAGCTGTGAT 
GCTGGTGCAA TATTAATTTT TCTACCTGGA TATGATGAAA TTGTTGGTTT GAGGGATCGT 
ATCCTCTTTG ATGACAAGCG GTTTGCTGAC AATACACATA GGTATCAAGT CTTTATGCTT 
CATTCAAATA TGCAGACATC TGATCAAAAG AAGGTATTAA AAAATCCACC TGCAGGTGTC 
CGAAAAATAA TCCTTTCCAC CAATATTGCT GAGACGAGCA TCACTGTCAA TGATGTTGTG 
TTTGTTATTG ATTCTGGTAA GGTGAAAGAG AAATCCTTTG ATGCACTGAA TTTTGTTACA 
ATGTTAAAAA TGGTATGGAT TTCCAAAGCT AGTGCAATAC AGCGCAAAGG CAGAGCGGGA 
CGGTGTAGAC CTGGAATTTG TTTCCGTCTG TTTAGTAGAC TTCGATTTCA GAACATGCTG 
GAATTTCAGA CGCCAGAACT CTTGAGAATG CCATTGCAGG AACTTTGTTT ACATACCAAG 
CTGTTAGCCC CAGTTAACTG CACCATTGCC GACTTCCTTA TGAAAGCCCC TGAACCGCCT 
CCAGCTTTAA TTGTGAGAAA TGCTGTGCAG ATGCTTAAGA CAATAGATGC AATGGATGCA 
TGGGAAGATC TGACTGAACT TGGGTATCAT TTGGCTGATT TGCCAGTAGA ACCGCATCTT 
GGGAAAATGG TCCTGTGTGC AGTGGTTTTG AAGTGTCTGG ACCCCATCCT TACAATTGCC 
TGCACGCTCG CTTACCGTGA CCCTTTTGTC CTGCCTACCC AGGCCTCTCA GAAACGGGCA 
GCTATGCTTT GTAGGAAACG CTTCACTGCA GGGACTTTCA GTGACCACAT GGCACTTCTG 
AGAGCATTTC AGGCATGGCA GAAAGCACGA AGTGATGGGT GGGAGCGAGC CTTTTGTGAA 
AAGAATTTTC TTTCACAAGC TACTATGGAA ATCATTATAG GCATGAGAAC ACAGTTGCTT 
GGTCAGCTTA GAGCATCAGG TTTTGTTAGA GCACGAGGTG GTGGTGACAT TCGAGATGTT 
AATACAAACT CCGAGAACTG GGCTGTTGTT AAAGCTGCAT TGGTAGCAGG CATGTATCCT 
AATTTAGTCC ACGTGGACAG AGAGAATGTA ATATTGACAG GGCCAAAGGA AAAGAAAGTA 
CGCTTTCATC CCACTTCAGT TCTCAGTCAA CCTCAATACA AAAAGATTCC TCCCGCCAAT 
GGTCAGGCTG CAGCGATTCA GGCATTGCCC ACAGATTGGC TTATTTATGA TGAAATGACC 
AGAGCCCATC GAATTGCTAA TATCAGATGT TGTTCAGCAG TGACACCTGT TACTGTCCTG 
GTGTTCTGTG GACCAGCAAG ACTGGCAAGT AACGCTCTTC AGGAACCTTC ATCCTTTAGA 
GCGGATGGTA TTCCCAATGA CAGCAGTGAC AGTGAGATGG AAGACAGAAC TACTGCCAAT 
TTGGCTGCTT TGAAGCTTGA TGAATGGCTT AACTTCAAAC TAGAGCCAGA GGCTGCCAGT 
TTATTGCTAC AGCTCAGACA GAAGTGGCAT AGTTTGTTTT TACGCCGAAT GAGAGCGCCA 
TCTAAACCTT GGTCTCAAGT CGATGAAGCT ACCATACGAG CAATTATAGC TGTTTTAAGC 
ACTGAAGAAC AGTCTGCAGG TTTACAGCAG CCATCTGGAA TTGGCCAGCG GCCAAGACCC 
ATGTCTTCAG AAGAACTTCC TTTGGCTTCA TCTTGGAGAT CCAATAATAG TAGGAAAAGT 
ACAGCTGACA CTGAGTTTGC GGATGGATCT ACTACTGGAG AAAGAGTACT GATGAAATCT 
CCATCTCCAG CCTTACATCC ACCTCAGAAG TACAAAGATA GAGGAATTTT ACATCCTAAG 
CGGAGCACCG ATGACCGGTC CGATCAATCA TCTGTGAAAT CCACAGACAG CAGCAGTTAC 
CCAAGTCCTT GTGCCAGCCC CTCTCCTCCA TCCTCAGGGA AGGGATCAAA ATCTCCTTCA 
CCAAGACCAA ACATGCCTAT TCGTTACTTC ATAATGAAGA GTAGCAATCT GAGAAACCTT 
GAAATTTCTC AACAGAAGGG TATCTGGTCT ACAACCCCTA GTAATGAGCG GAAGCTAAAT 
CGAGCGTTTT GGGAGAGCAG CATGGTTTAC TTGGTATTTT CTGTTCAAGG ATCTGGACAT 
TTCCAGGGAT TTTCTAGGAT GTCTTCTGAG ATAGGAAGGG AGAAGAGCCA GGACTGGGGG 
TCAGCTGGAC TAGGTGGAGT ATTTAAAGTG GAGTGGATAC GCAAAGAAAG CCTTCCCTTT 
CAGTTTGCAC ACCATTTACT CAATCCTTGG AACGACAACA AGAAAGTACA GATAAGCAGA 
GATGGGCAGG AGCTAGAACC TCAGGTTGGG GAACAGTTGC TCCAGTTATG GGAACGCCTT 
CCATTGGGAG AAAAAACAAC TTCTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ythdc2 ( XM_030250454.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106314.1 Mus musculus YTH domain containing 2 (Ythdc2), transcript variant X2, mRNA.

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CloneID	OMu87394
Clone ID Related Accession (Same CDS sequence)	XM_030250454.1
Accession Version	XM_030250454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	3'-5' RNA helicase YTHDC2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGTG ATAGCACATT GTCAACTGTG ACCCATGTTA TTGTGGATGA AGTGCATGAA 
AGGGATCGAT TCAGTGATTT TTTGCTTACT AAGTTAAGAG ATTTGTTGCA AAAGCACCCA 
ACTTTGAAAC TAATTCTTTC GAGTGCTGCC TTGGATGTGA ACCTCTTCAT AAGATATTTT 
GGAAGTTGTC CAGTGATATA TATACAGGGA AGACCATTTG AAGTAAAAGA AATGTTTCTG 
GAAGATATTT TAAGAACTAC AGGATATACC AACAAAGAAA TGTTAAAGTA CAAAAAGGAA 
AAGCAACGAG AGGAGAAACA ACAGACCACC CTCACAGAGT GGTACTCAGC TCAAGAGAAT 
ACCTTCAAGC CTGAGTCTCA GAGGCAGAGA GCTGTTGCTA GTGTGTCTGA AGAGTATGAC 
TTATTGGATG ATGGAGGTGA TGCTGTCTTC AGTCAGCTGA CAGAAAAAGA TGTGAATTGC 
CTTGAACCAT GGTTAATCAA GGAGATGGAT GCCTGCCTTT CTGACATATG GCTGCATAAA 
GATGTTGATG CTTTTGCTCA GGTCTTTCAT CTTATTTTAA CTGAAAATGT TAGTGTTGAT 
TATAGGCATA GTGAGACCAG TGCAACAGCT CTGATGGTTG CTGCAGGACG GGGCTTTACG 
AGCCAAGTAG AGCAGTTAAT TAGTATGGGA GCCAATGTCC ACAGTAAAGC ATCAAATGGC 
TGGATGGCTT TAGATTGGGC TAAGCACTTT GGACAGACTG AAATTGTGGA TCTTCTAGAA 
TCTTACAGTG CTTCATTGGA ATTTGGAAAT CTAGATGAAA GTTCCTTGGT TCAGACAAAT 
GGGAATGACC TCAGTGCAGA AGACAGAGAG CTGTTGAAAG CTTACCATCA TAGTTTTGAT 
GATGAGAAAG TAGACTTGGA TTTGATCATG CATCTCCTAT ATAATATCTG CCATAGCTGT 
GATGCTGGTG CAATATTAAT TTTTCTACCT GGATATGATG AAATTGTTGG TTTGAGGGAT 
CGTATCCTCT TTGATGACAA GCGGTTTGCT GACAATACAC ATAGGTATCA AGTCTTTATG 
CTTCATTCAA ATATGCAGAC ATCTGATCAA AAGAAGGTAT TAAAAAATCC ACCTGCAGGT 
GTCCGAAAAA TAATCCTTTC CACCAATATT GCTGAGACGA GCATCACTGT CAATGATGTT 
GTGTTTGTTA TTGATTCTGG TAAGGTGAAA GAGAAATCCT TTGATGCACT GAATTTTGTT 
ACAATGTTAA AAATGGTATG GATTTCCAAA GCTAGTGCAA TACAGCGCAA AGGCAGAGCG 
GGACGGTGTA GACCTGGAAT TTGTTTCCGT CTGTTTAGTA GACTTCGATT TCAGAACATG 
CTGGAATTTC AGACGCCAGA ACTCTTGAGA ATGCCATTGC AGGAACTTTG TTTACATACC 
AAGCTGTTAG CCCCAGTTAA CTGCACCATT GCCGACTTCC TTATGAAAGC CCCTGAACCG 
CCTCCAGCTT TAATTGTGAG AAATGCTGTG CAGATGCTTA AGACAATAGA TGCAATGGAT 
GCATGGGAAG ATCTGACTGA ACTTGGGTAT CATTTGGCTG ATTTGCCAGT AGAACCGCAT 
CTTGGGAAAA TGGTCCTGTG TGCAGTGGTT TTGAAGTGTC TGGACCCCAT CCTTACAATT 
GCCTGCACGC TCGCTTACCG TGACCCTTTT GTCCTGCCTA CCCAGGCCTC TCAGAAACGG 
GCAGCTATGC TTTGTAGGAA ACGCTTCACT GCAGGGACTT TCAGTGACCA CATGGCACTT 
CTGAGAGCAT TTCAGGCATG GCAGAAAGCA CGAAGTGATG GGTGGGAGCG AGCCTTTTGT 
GAAAAGAATT TTCTTTCACA AGCTACTATG GAAATCATTA TAGGCATGAG AACACAGTTG 
CTTGGTCAGC TTAGAGCATC AGGTTTTGTT AGAGCACGAG GTGGTGGTGA CATTCGAGAT 
GTTAATACAA ACTCCGAGAA CTGGGCTGTT GTTAAAGCTG CATTGGTAGC AGGCATGTAT 
CCTAATTTAG TCCACGTGGA CAGAGAGAAT GTAATATTGA CAGGGCCAAA GGAAAAGAAA 
GTACGCTTTC ATCCCACTTC AGTTCTCAGT CAACCTCAAT ACAAAAAGAT TCCTCCCGCC 
AATGGTCAGG CTGCAGCGAT TCAGGCATTG CCCACAGATT GGCTTATTTA TGATGAAATG 
ACCAGAGCCC ATCGAATTGC TAATATCAGA TGTTGTTCAG CAGTGACACC TGTTACTGTC 
CTGGTGTTCT GTGGACCAGC AAGACTGGCA AGTAACGCTC TTCAGGAACC TTCATCCTTT 
AGAGCGGATG GTATTCCCAA TGACAGCAGT GACAGTGAGA TGGAAGACAG AACTACTGCC 
AATTTGGCTG CTTTGAAGCT TGATGAATGG CTTAACTTCA AACTAGAGCC AGAGGCTGCC 
AGTTTATTGC TACAGCTCAG ACAGAAGTGG CATAGTTTGT TTTTACGCCG AATGAGAGCG 
CCATCTAAAC CTTGGTCTCA AGTCGATGAA GCTACCATAC GAGCAATTAT AGCTGTTTTA 
AGCACTGAAG AACAGTCTGC AGGTTTACAG CAGCCATCTG GAATTGGCCA GCGGCCAAGA 
CCCATGTCTT CAGAAGAACT TCCTTTGGCT TCATCTTGGA GATCCAATAA TAGTAGGAAA 
AGTACAGCTG ACACTGAGTT TGCGGATGGA TCTACTACTG GAGAAAGAGT ACTGATGAAA 
TCTCCATCTC CAGCCTTACA TCCACCTCAG AAGTACAAAG ATAGAGGAAT TTTACATCCT 
AAGCGGAGCA CCGATGACCG GTCCGATCAA TCATCTGTGA AATCCACAGA CAGCAGCAGT 
TACCCAAGTC CTTGTGCCAG CCCCTCTCCT CCATCCTCAG GGAAGGGATC AAAATCTCCT 
TCACCAAGAC CAAACATGCC TATTCGTTAC TTCATAATGA AGAGTAGCAA TCTGAGAAAC 
CTTGAAATTT CTCAACAGAA GGGTATCTGG TCTACAACCC CTAGTAATGA GCGGAAGCTA 
AATCGAGCGT TTTGGGAGAG CAGCATGGTT TACTTGGTAT TTTCTGTTCA AGGATCTGGA 
CATTTCCAGG GATTTTCTAG GATGTCTTCT GAGATAGGAA GGGAGAAGAG CCAGGACTGG 
GGGTCAGCTG GACTAGGTGG AGTATTTAAA GTGGAGTGGA TACGCAAAGA AAGCCTTCCC 
TTTCAGTTTG CACACCATTT ACTCAATCCT TGGAACGACA ACAAGAAAGT ACAGATAAGC 
AGAGATGGGC AGGAGCTAGA ACCTCAGGTT GGGGAACAGT TGCTCCAGTT ATGGGAACGC 
CTTCCATTGG GAGAAAAAAC AACTTCTGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106314.1
CDS	751..4143
Translation	MAGDSTLSTVTHVIVDEVHERDRFSDFLLTKLRDLLQKHPTLKLILSSAALDVNLFIRYFGSCPVIYIQGRPFEVKEMFLEDILRTTGYTNKEMLKYKKEKQREEKQQTTLTEWYSAQENTFKPESQRQRAVASVSEEYDLLDDGGDAVFSQLTEKDVNCLEPWLIKEMDACLSDIWLHKDVDAFAQVFHLILTENVSVDYRHSETSATALMVAAGRGFTSQVEQLISMGANVHSKASNGWMALDWAKHFGQTEIVDLLESYSASLEFGNLDESSLVQTNGNDLSAEDRELLKAYHHSFDDEKVDLDLIMHLLYNICHSCDAGAILIFLPGYDEIVGLRDRILFDDKRFADNTHRYQVFMLHSNMQTSDQKKVLKNPPAGVRKIILSTNIAETSITVNDVVFVIDSGKVKEKSFDALNFVTMLKMVWISKASAIQRKGRAGRCRPGICFRLFSRLRFQNMLEFQTPELLRMPLQELCLHTKLLAPVNCTIADFLMKAPEPPPALIVRNAVQMLKTIDAMDAWEDLTELGYHLADLPVEPHLGKMVLCAVVLKCLDPILTIACTLAYRDPFVLPTQASQKRAAMLCRKRFTAGTFSDHMALLRAFQAWQKARSDGWERAFCEKNFLSQATMEIIIGMRTQLLGQLRASGFVRARGGGDIRDVNTNSENWAVVKAALVAGMYPNLVHVDRENVILTGPKEKKVRFHPTSVLSQPQYKKIPPANGQAAAIQALPTDWLIYDEMTRAHRIANIRCCSAVTPVTVLVFCGPARLASNALQEPSSFRADGIPNDSSDSEMEDRTTANLAALKLDEWLNFKLEPEAASLLLQLRQKWHSLFLRRMRAPSKPWSQVDEATIRAIIAVLSTEEQSAGLQQPSGIGQRPRPMSSEELPLASSWRSNNSRKSTADTEFADGSTTGERVLMKSPSPALHPPQKYKDRGILHPKRSTDDRSDQSSVKSTDSSSYPSPCASPSPPSSGKGSKSPSPRPNMPIRYFIMKSSNLRNLEISQQKGIWSTTPSNERKLNRAFWESSMVYLVFSVQGSGHFQGFSRMSSEIGREKSQDWGSAGLGGVFKVEWIRKESLPFQFAHHLLNPWNDNKKVQISRDGQELEPQVGEQLLQLWERLPLGEKTTSD

Target ORF information:

RefSeq Version	XM_030250454.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus YTH domain containing 2 (Ythdc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250454.1

ORF Insert Sequence:

ATGGCAGGTG ATAGCACATT GTCAACTGTG ACCCATGTTA TTGTGGATGA AGTGCATGAA 
AGGGATCGAT TCAGTGATTT TTTGCTTACT AAGTTAAGAG ATTTGTTGCA AAAGCACCCA 
ACTTTGAAAC TAATTCTTTC GAGTGCTGCC TTGGATGTGA ACCTCTTCAT AAGATATTTT 
GGAAGTTGTC CAGTGATATA TATACAGGGA AGACCATTTG AAGTAAAAGA AATGTTTCTG 
GAAGATATTT TAAGAACTAC AGGATATACC AACAAAGAAA TGTTAAAGTA CAAAAAGGAA 
AAGCAACGAG AGGAGAAACA ACAGACCACC CTCACAGAGT GGTACTCAGC TCAAGAGAAT 
ACCTTCAAGC CTGAGTCTCA GAGGCAGAGA GCTGTTGCTA GTGTGTCTGA AGAGTATGAC 
TTATTGGATG ATGGAGGTGA TGCTGTCTTC AGTCAGCTGA CAGAAAAAGA TGTGAATTGC 
CTTGAACCAT GGTTAATCAA GGAGATGGAT GCCTGCCTTT CTGACATATG GCTGCATAAA 
GATGTTGATG CTTTTGCTCA GGTCTTTCAT CTTATTTTAA CTGAAAATGT TAGTGTTGAT 
TATAGGCATA GTGAGACCAG TGCAACAGCT CTGATGGTTG CTGCAGGACG GGGCTTTACG 
AGCCAAGTAG AGCAGTTAAT TAGTATGGGA GCCAATGTCC ACAGTAAAGC ATCAAATGGC 
TGGATGGCTT TAGATTGGGC TAAGCACTTT GGACAGACTG AAATTGTGGA TCTTCTAGAA 
TCTTACAGTG CTTCATTGGA ATTTGGAAAT CTAGATGAAA GTTCCTTGGT TCAGACAAAT 
GGGAATGACC TCAGTGCAGA AGACAGAGAG CTGTTGAAAG CTTACCATCA TAGTTTTGAT 
GATGAGAAAG TAGACTTGGA TTTGATCATG CATCTCCTAT ATAATATCTG CCATAGCTGT 
GATGCTGGTG CAATATTAAT TTTTCTACCT GGATATGATG AAATTGTTGG TTTGAGGGAT 
CGTATCCTCT TTGATGACAA GCGGTTTGCT GACAATACAC ATAGGTATCA AGTCTTTATG 
CTTCATTCAA ATATGCAGAC ATCTGATCAA AAGAAGGTAT TAAAAAATCC ACCTGCAGGT 
GTCCGAAAAA TAATCCTTTC CACCAATATT GCTGAGACGA GCATCACTGT CAATGATGTT 
GTGTTTGTTA TTGATTCTGG TAAGGTGAAA GAGAAATCCT TTGATGCACT GAATTTTGTT 
ACAATGTTAA AAATGGTATG GATTTCCAAA GCTAGTGCAA TACAGCGCAA AGGCAGAGCG 
GGACGGTGTA GACCTGGAAT TTGTTTCCGT CTGTTTAGTA GACTTCGATT TCAGAACATG 
CTGGAATTTC AGACGCCAGA ACTCTTGAGA ATGCCATTGC AGGAACTTTG TTTACATACC 
AAGCTGTTAG CCCCAGTTAA CTGCACCATT GCCGACTTCC TTATGAAAGC CCCTGAACCG 
CCTCCAGCTT TAATTGTGAG AAATGCTGTG CAGATGCTTA AGACAATAGA TGCAATGGAT 
GCATGGGAAG ATCTGACTGA ACTTGGGTAT CATTTGGCTG ATTTGCCAGT AGAACCGCAT 
CTTGGGAAAA TGGTCCTGTG TGCAGTGGTT TTGAAGTGTC TGGACCCCAT CCTTACAATT 
GCCTGCACGC TCGCTTACCG TGACCCTTTT GTCCTGCCTA CCCAGGCCTC TCAGAAACGG 
GCAGCTATGC TTTGTAGGAA ACGCTTCACT GCAGGGACTT TCAGTGACCA CATGGCACTT 
CTGAGAGCAT TTCAGGCATG GCAGAAAGCA CGAAGTGATG GGTGGGAGCG AGCCTTTTGT 
GAAAAGAATT TTCTTTCACA AGCTACTATG GAAATCATTA TAGGCATGAG AACACAGTTG 
CTTGGTCAGC TTAGAGCATC AGGTTTTGTT AGAGCACGAG GTGGTGGTGA CATTCGAGAT 
GTTAATACAA ACTCCGAGAA CTGGGCTGTT GTTAAAGCTG CATTGGTAGC AGGCATGTAT 
CCTAATTTAG TCCACGTGGA CAGAGAGAAT GTAATATTGA CAGGGCCAAA GGAAAAGAAA 
GTACGCTTTC ATCCCACTTC AGTTCTCAGT CAACCTCAAT ACAAAAAGAT TCCTCCCGCC 
AATGGTCAGG CTGCAGCGAT TCAGGCATTG CCCACAGATT GGCTTATTTA TGATGAAATG 
ACCAGAGCCC ATCGAATTGC TAATATCAGA TGTTGTTCAG CAGTGACACC TGTTACTGTC 
CTGGTGTTCT GTGGACCAGC AAGACTGGCA AGTAACGCTC TTCAGGAACC TTCATCCTTT 
AGAGCGGATG GTATTCCCAA TGACAGCAGT GACAGTGAGA TGGAAGACAG AACTACTGCC 
AATTTGGCTG CTTTGAAGCT TGATGAATGG CTTAACTTCA AACTAGAGCC AGAGGCTGCC 
AGTTTATTGC TACAGCTCAG ACAGAAGTGG CATAGTTTGT TTTTACGCCG AATGAGAGCG 
CCATCTAAAC CTTGGTCTCA AGTCGATGAA GCTACCATAC GAGCAATTAT AGCTGTTTTA 
AGCACTGAAG AACAGTCTGC AGGTTTACAG CAGCCATCTG GAATTGGCCA GCGGCCAAGA 
CCCATGTCTT CAGAAGAACT TCCTTTGGCT TCATCTTGGA GATCCAATAA TAGTAGGAAA 
AGTACAGCTG ACACTGAGTT TGCGGATGGA TCTACTACTG GAGAAAGAGT ACTGATGAAA 
TCTCCATCTC CAGCCTTACA TCCACCTCAG AAGTACAAAG ATAGAGGAAT TTTACATCCT 
AAGCGGAGCA CCGATGACCG GTCCGATCAA TCATCTGTGA AATCCACAGA CAGCAGCAGT 
TACCCAAGTC CTTGTGCCAG CCCCTCTCCT CCATCCTCAG GGAAGGGATC AAAATCTCCT 
TCACCAAGAC CAAACATGCC TATTCGTTAC TTCATAATGA AGAGTAGCAA TCTGAGAAAC 
CTTGAAATTT CTCAACAGAA GGGTATCTGG TCTACAACCC CTAGTAATGA GCGGAAGCTA 
AATCGAGCGT TTTGGGAGAG CAGCATGGTT TACTTGGTAT TTTCTGTTCA AGGATCTGGA 
CATTTCCAGG GATTTTCTAG GATGTCTTCT GAGATAGGAA GGGAGAAGAG CCAGGACTGG 
GGGTCAGCTG GACTAGGTGG AGTATTTAAA GTGGAGTGGA TACGCAAAGA AAGCCTTCCC 
TTTCAGTTTG CACACCATTT ACTCAATCCT TGGAACGACA ACAAGAAAGT ACAGATAAGC 
AGAGATGGGC AGGAGCTAGA ACCTCAGGTT GGGGAACAGT TGCTCCAGTT ATGGGAACGC 
CTTCCATTGG GAGAAAAAAC AACTTCTGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ythdc2 ( NM_001163013.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156485.1 Mus musculus YTH domain containing 2 (Ythdc2), mRNA.

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CloneID	OMu08687
Clone ID Related Accession (Same CDS sequence)	NM_001163013.1
Accession Version	NM_001163013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Mus musculus(house mouse)
Product	3'-5' RNA helicase YTHDC2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC137150.11 and AC134472.4. On or before Jul 8, 2009 this sequence version replaced XM_910737.2, XM_140310.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC138263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849390, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGGC CGAGCAGCGT CTCGCCGCGT CCGCCGGCTC CTAGCGGAGG CGGCACGGGC 
GGCGGTGGAG GTGGCAGTGG CGGCGGCGGT GGCGGTGGCG GCGGCGGCCC AGCTTCGTGT 
GGTCCTGGAG GCGGTGGCCG GGCCAAGGGC CTCAAAGACA TTCGTATCGA CGAGGAGGTG 
AAGATCGCCG TCAACATCGC TCTGGAGCGC TTCCGCTACG GTGACCAGAG AGAAATGGAA 
TTCCCTTCTT CTTTGACCAG TACGGAAAGA GCCTTTATTC ACCGACTAAG TCAATCTCTT 
GGTTTAGTTT CTAAAAGTAA AGGAAAGGGA GCAAATAGAT ACCTAACTGT GAAGAAGAAA 
GATGGATCAG AAACAGCTCA TGCAATGATG ACCTGTAATT TGACTCATAA CACAAAACAT 
GCTGTTAGGA GCCTAATTCA GAGATTTCCT GTCACCAATA AAGAACGTAC TGAACTCCTA 
CCTAAAACAG AAAGAGGAAA TGTGTTTGCA GTTGAAGCTG AAAACCGAGA AATGAGCAAG 
ACAAGCGGGC GACTCAACAA TGGCATTCCT CAAGTTCCTG TGAAAAGGGG AGAGTCTGAA 
TTTGACTCAT TCAGGCAGTC TTTACCAGTT TTTGAGAAAC AAGAAGAAAT TGTTAAAATA 
ATTAAGGAAA ATAAAGTAGT TTTGATAGTA GGAGAAACTG GGTCTGGAAA GACCACACAG 
ATTCCTCAGT TCCTGTTAGA TGATTGCTTT AAAAATGGCA TCCCCTGCCG AATATTTTGT 
ACTCAACCAA GACGACTAGC AGCGATTGCT GTGGCTGAAA GAGTTGCTGC AGAGAGAAGA 
GAAAGAATTG GTCAAACAAT TGGTTATCAG ATCCGATTAG AAAGCAGGGT TTCGCCAAAG 
ACACTTCTGA CATTTTGCAC TAATGGGGTA TTGCTTCGTA CATTGATGGC AGGTGATAGC 
ACATTGTCAA CTGTGACCCA TGTTATTGTG GATGAAGTGC ATGAAAGGGA TCGATTCAGT 
GATTTTTTGC TTACTAAGTT AAGAGATTTG TTGCAAAAGC ACCCAACTTT GAAACTAATT 
CTTTCGAGTG CTGCCTTGGA TGTGAACCTC TTCATAAGAT ATTTTGGAAG TTGTCCAGTG 
ATATATATAC AGGGAAGACC ATTTGAAGTA AAAGAAATGT TTCTGGAAGA TATTTTAAGA 
ACTACAGGAT ATACCAACAA AGAAATGTTA AAGTACAAAA AGGAAAAGCA ACGAGAGGAG 
AAACAACAGA CCACCCTCAC AGAGTGGTAC TCAGCTCAAG AGAATACCTT CAAGCCTGAG 
TCTCAGAGGC AGAGAGCTGT TGCTAGTGTG TCTGAAGAGT ATGACTTATT GGATGATGGA 
GGTGATGCTG TCTTCAGTCA GCTGACAGAA AAAGATGTGA ATTGCCTTGA ACCATGGTTA 
ATCAAGGAGA TGGATGCCTG CCTTTCTGAC ATATGGCTGC ATAAAGATGT TGATGCTTTT 
GCTCAGGTCT TTCATCTTAT TTTAACTGAA AATGTTAGTG TTGATTATAG GCATAGTGAG 
ACCAGTGCAA CAGCTCTGAT GGTTGCTGCA GGACGGGGCT TTACGAGCCA AGTAGAGCAG 
TTAATTAGTA TGGGAGCCAA TGTCCACAGT AAAGCATCAA ATGGCTGGAT GGCTTTAGAT 
TGGGCTAAGC ACTTTGGACA GACTGAAATT GTGGATCTTC TAGAATCTTA CAGTGCTTCA 
TTGGAATTTG GAAATCTAGA TGAAAGTTCC TTGGTTCAGA CAAATGGGAA TGACCTCAGT 
GCAGAAGACA GAGAGCTGTT GAAAGCTTAC CATCATAGTT TTGATGATGA GAAAGTAGAC 
TTGGATTTGA TCATGCATCT CCTATATAAT ATCTGCCATA GCTGTGATGC TGGTGCAATA 
TTAATTTTTC TACCTGGATA TGATGAAATT GTTGGTTTGA GGGATCGTAT CCTCTTTGAT 
GACAAGCGGT TTGCTGACAA TACACATAGG TATCAAGTCT TTATGCTTCA TTCAAATATG 
CAGACATCTG ATCAAAAGAA GGTATTAAAA AATCCACCTG CAGGTGTCCG AAAAATAATC 
CTTTCCACCA ATATTGCTGA GACGAGCATC ACTGTCAATG ATGTTGTGTT TGTTATTGAT 
TCTGGTAAGG TGAAAGAGAA ATCCTTTGAT GCACTGAATT TTGTTACAAT GTTAAAAATG 
GTATGGATTT CCAAAGCTAG TGCAATACAG CGCAAAGGCA GAGCGGGACG GTGTAGACCT 
GGAATTTGTT TCCGTCTGTT TAGTAGACTT CGATTTCAGA ACATGCTGGA ATTTCAGACG 
CCAGAACTCT TGAGAATGCC ATTGCAGGAA CTTTGTTTAC ATACCAAGCT GTTAGCCCCA 
GTTAACTGCA CCATTGCCGA CTTCCTTATG AAAGCCCCTG AACCGCCTCC AGCTTTAATT 
GTGAGAAATG CTGTGCAGAT GCTTAAGACA ATAGATGCAA TGGATGCATG GGAAGATCTG 
ACTGAACTTG GGTATCATTT GGCTGATTTG CCAGTAGAAC CGCATCTTGG GAAAATGGTC 
CTGTGTGCAG TGGTTTTGAA GTGTCTGGAC CCCATCCTTA CAATTGCCTG CACGCTCGCT 
TACCGTGACC CTTTTGTCCT GCCTACCCAG GCCTCTCAGA AACGGGCAGC TATGCTTTGT 
AGGAAACGCT TCACTGCAGG GACTTTCAGT GACCACATGG CACTTCTGAG AGCATTTCAG 
GCATGGCAGA AAGCACGAAG TGATGGGTGG GAGCGAGCCT TTTGTGAAAA GAATTTTCTT 
TCACAAGCTA CTATGGAAAT CATTATAGGC ATGAGAACAC AGTTGCTTGG TCAGCTTAGA 
GCATCAGGTT TTGTTAGAGC ACGAGGTGGT GGTGACATTC GAGATGTTAA TACAAACTCC 
GAGAACTGGG CTGTTGTTAA AGCTGCATTG GTAGCAGGCA TGTATCCTAA TTTAGTCCAC 
GTGGACAGAG AGAATGTAAT ATTGACAGGG CCAAAGGAAA AGAAAGTACG CTTTCATCCC 
ACTTCAGTTC TCAGTCAACC TCAATACAAA AAGATTCCTC CCGCCAATGG TCAGGCTGCA 
GCGATTCAGG CATTGCCCAC AGATTGGCTT ATTTATGATG AAATGACCAG AGCCCATCGA 
ATTGCTAATA TCAGATGTTG TTCAGCAGTG ACACCTGTTA CTGTCCTGGT GTTCTGTGGA 
CCAGCAAGAC TGGCAAGTAA CGCTCTTCAG GAACCTTCAT CCTTTAGAGC GGATGGTATT 
CCCAATGACA GCAGTGACAG TGAGATGGAA GACAGAACTA CTGCCAATTT GGCTGCTTTG 
AAGCTTGATG AATGGCTTAA CTTCAAACTA GAGCCAGAGG CTGCCAGTTT ATTGCTACAG 
CTCAGACAGA AGTGGCATAG TTTGTTTTTA CGCCGAATGA GAGCGCCATC TAAACCTTGG 
TCTCAAGTCG ATGAAGCTAC CATACGAGCA ATTATAGCTG TTTTAAGCAC TGAAGAACAG 
TCTGCAGGTT TACAGCAGCC ATCTGGAATT GGCCAGCGGC CAAGACCCAT GTCTTCAGAA 
GAACTTCCTT TGGCTTCATC TTGGAGATCC AATAATAGTA GGAAAAGTAC AGCTGACACT 
GAGTTTGCGG ATGGATCTAC TACTGGAGAA AGAGTACTGA TGAAATCTCC ATCTCCAGCC 
TTACATCCAC CTCAGAAGTA CAAAGATAGA GGAATTTTAC ATCCTAAGCG GAGCACCGAT 
GACCGGTCCG ATCAATCATC TGTGAAATCC ACAGACAGCA GCAGTTACCC AAGTCCTTGT 
GCCAGCCCCT CTCCTCCATC CTCAGGGAAG GGATCAAAAT CTCCTTCACC AAGACCAAAC 
ATGCCTATTC GTTACTTCAT AATGAAGAGT AGCAATCTGA GAAACCTTGA AATTTCTCAA 
CAGAAGGGTA TCTGGTCTAC AACCCCTAGT AATGAGCGGA AGCTAAATCG AGCGTTTTGG 
GAGAGCAGCA TGGTTTACTT GGTATTTTCT GTTCAAGGAT CTGGACATTT CCAGGGATTT 
TCTAGGATGT CTTCTGAGAT AGGAAGGGAG AAGAGCCAGG ACTGGGGGTC AGCTGGACTA 
GGTGGAGTAT TTAAAGTGGA GTGGATACGC AAAGAAAGCC TTCCCTTTCA GTTTGCACAC 
CATTTACTCA ATCCTTGGAA CGACAACAAG AAAGTACAGA TAAGCAGAGA TGGGCAGGAG 
CTAGAACCTC AGGTTGGGGA ACAGTTGCTC CAGTTATGGG AACGCCTTCC ATTGGGAGAA 
AAAACAACTT CTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156485.1
CDS	23..4360
Translation	MSRPSSVSPRPPAPSGGGTGGGGGGSGGGGGGGGGGPASCGPGGGGRAKGLKDIRIDEEVKIAVNIALERFRYGDQREMEFPSSLTSTERAFIHRLSQSLGLVSKSKGKGANRYLTVKKKDGSETAHAMMTCNLTHNTKHAVRSLIQRFPVTNKERTELLPKTERGNVFAVEAENREMSKTSGRLNNGIPQVPVKRGESEFDSFRQSLPVFEKQEEIVKIIKENKVVLIVGETGSGKTTQIPQFLLDDCFKNGIPCRIFCTQPRRLAAIAVAERVAAERRERIGQTIGYQIRLESRVSPKTLLTFCTNGVLLRTLMAGDSTLSTVTHVIVDEVHERDRFSDFLLTKLRDLLQKHPTLKLILSSAALDVNLFIRYFGSCPVIYIQGRPFEVKEMFLEDILRTTGYTNKEMLKYKKEKQREEKQQTTLTEWYSAQENTFKPESQRQRAVASVSEEYDLLDDGGDAVFSQLTEKDVNCLEPWLIKEMDACLSDIWLHKDVDAFAQVFHLILTENVSVDYRHSETSATALMVAAGRGFTSQVEQLISMGANVHSKASNGWMALDWAKHFGQTEIVDLLESYSASLEFGNLDESSLVQTNGNDLSAEDRELLKAYHHSFDDEKVDLDLIMHLLYNICHSCDAGAILIFLPGYDEIVGLRDRILFDDKRFADNTHRYQVFMLHSNMQTSDQKKVLKNPPAGVRKIILSTNIAETSITVNDVVFVIDSGKVKEKSFDALNFVTMLKMVWISKASAIQRKGRAGRCRPGICFRLFSRLRFQNMLEFQTPELLRMPLQELCLHTKLLAPVNCTIADFLMKAPEPPPALIVRNAVQMLKTIDAMDAWEDLTELGYHLADLPVEPHLGKMVLCAVVLKCLDPILTIACTLAYRDPFVLPTQASQKRAAMLCRKRFTAGTFSDHMALLRAFQAWQKARSDGWERAFCEKNFLSQATMEIIIGMRTQLLGQLRASGFVRARGGGDIRDVNTNSENWAVVKAALVAGMYPNLVHVDRENVILTGPKEKKVRFHPTSVLSQPQYKKIPPANGQAAAIQALPTDWLIYDEMTRAHRIANIRCCSAVTPVTVLVFCGPARLASNALQEPSSFRADGIPNDSSDSEMEDRTTANLAALKLDEWLNFKLEPEAASLLLQLRQKWHSLFLRRMRAPSKPWSQVDEATIRAIIAVLSTEEQSAGLQQPSGIGQRPRPMSSEELPLASSWRSNNSRKSTADTEFADGSTTGERVLMKSPSPALHPPQKYKDRGILHPKRSTDDRSDQSSVKSTDSSSYPSPCASPSPPSSGKGSKSPSPRPNMPIRYFIMKSSNLRNLEISQQKGIWSTTPSNERKLNRAFWESSMVYLVFSVQGSGHFQGFSRMSSEIGREKSQDWGSAGLGGVFKVEWIRKESLPFQFAHHLLNPWNDNKKVQISRDGQELEPQVGEQLLQLWERLPLGEKTTSD

Target ORF information:

RefSeq Version	NM_001163013.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus YTH domain containing 2 (Ythdc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163013.1

ORF Insert Sequence:

ATGTCCAGGC CGAGCAGCGT CTCGCCGCGT CCGCCGGCTC CTAGCGGAGG CGGCACGGGC 
GGCGGTGGAG GTGGCAGTGG CGGCGGCGGT GGCGGTGGCG GCGGCGGCCC AGCTTCGTGT 
GGTCCTGGAG GCGGTGGCCG GGCCAAGGGC CTCAAAGACA TTCGTATCGA CGAGGAGGTG 
AAGATCGCCG TCAACATCGC TCTGGAGCGC TTCCGCTACG GTGACCAGAG AGAAATGGAA 
TTCCCTTCTT CTTTGACCAG TACGGAAAGA GCCTTTATTC ACCGACTAAG TCAATCTCTT 
GGTTTAGTTT CTAAAAGTAA AGGAAAGGGA GCAAATAGAT ACCTAACTGT GAAGAAGAAA 
GATGGATCAG AAACAGCTCA TGCAATGATG ACCTGTAATT TGACTCATAA CACAAAACAT 
GCTGTTAGGA GCCTAATTCA GAGATTTCCT GTCACCAATA AAGAACGTAC TGAACTCCTA 
CCTAAAACAG AAAGAGGAAA TGTGTTTGCA GTTGAAGCTG AAAACCGAGA AATGAGCAAG 
ACAAGCGGGC GACTCAACAA TGGCATTCCT CAAGTTCCTG TGAAAAGGGG AGAGTCTGAA 
TTTGACTCAT TCAGGCAGTC TTTACCAGTT TTTGAGAAAC AAGAAGAAAT TGTTAAAATA 
ATTAAGGAAA ATAAAGTAGT TTTGATAGTA GGAGAAACTG GGTCTGGAAA GACCACACAG 
ATTCCTCAGT TCCTGTTAGA TGATTGCTTT AAAAATGGCA TCCCCTGCCG AATATTTTGT 
ACTCAACCAA GACGACTAGC AGCGATTGCT GTGGCTGAAA GAGTTGCTGC AGAGAGAAGA 
GAAAGAATTG GTCAAACAAT TGGTTATCAG ATCCGATTAG AAAGCAGGGT TTCGCCAAAG 
ACACTTCTGA CATTTTGCAC TAATGGGGTA TTGCTTCGTA CATTGATGGC AGGTGATAGC 
ACATTGTCAA CTGTGACCCA TGTTATTGTG GATGAAGTGC ATGAAAGGGA TCGATTCAGT 
GATTTTTTGC TTACTAAGTT AAGAGATTTG TTGCAAAAGC ACCCAACTTT GAAACTAATT 
CTTTCGAGTG CTGCCTTGGA TGTGAACCTC TTCATAAGAT ATTTTGGAAG TTGTCCAGTG 
ATATATATAC AGGGAAGACC ATTTGAAGTA AAAGAAATGT TTCTGGAAGA TATTTTAAGA 
ACTACAGGAT ATACCAACAA AGAAATGTTA AAGTACAAAA AGGAAAAGCA ACGAGAGGAG 
AAACAACAGA CCACCCTCAC AGAGTGGTAC TCAGCTCAAG AGAATACCTT CAAGCCTGAG 
TCTCAGAGGC AGAGAGCTGT TGCTAGTGTG TCTGAAGAGT ATGACTTATT GGATGATGGA 
GGTGATGCTG TCTTCAGTCA GCTGACAGAA AAAGATGTGA ATTGCCTTGA ACCATGGTTA 
ATCAAGGAGA TGGATGCCTG CCTTTCTGAC ATATGGCTGC ATAAAGATGT TGATGCTTTT 
GCTCAGGTCT TTCATCTTAT TTTAACTGAA AATGTTAGTG TTGATTATAG GCATAGTGAG 
ACCAGTGCAA CAGCTCTGAT GGTTGCTGCA GGACGGGGCT TTACGAGCCA AGTAGAGCAG 
TTAATTAGTA TGGGAGCCAA TGTCCACAGT AAAGCATCAA ATGGCTGGAT GGCTTTAGAT 
TGGGCTAAGC ACTTTGGACA GACTGAAATT GTGGATCTTC TAGAATCTTA CAGTGCTTCA 
TTGGAATTTG GAAATCTAGA TGAAAGTTCC TTGGTTCAGA CAAATGGGAA TGACCTCAGT 
GCAGAAGACA GAGAGCTGTT GAAAGCTTAC CATCATAGTT TTGATGATGA GAAAGTAGAC 
TTGGATTTGA TCATGCATCT CCTATATAAT ATCTGCCATA GCTGTGATGC TGGTGCAATA 
TTAATTTTTC TACCTGGATA TGATGAAATT GTTGGTTTGA GGGATCGTAT CCTCTTTGAT 
GACAAGCGGT TTGCTGACAA TACACATAGG TATCAAGTCT TTATGCTTCA TTCAAATATG 
CAGACATCTG ATCAAAAGAA GGTATTAAAA AATCCACCTG CAGGTGTCCG AAAAATAATC 
CTTTCCACCA ATATTGCTGA GACGAGCATC ACTGTCAATG ATGTTGTGTT TGTTATTGAT 
TCTGGTAAGG TGAAAGAGAA ATCCTTTGAT GCACTGAATT TTGTTACAAT GTTAAAAATG 
GTATGGATTT CCAAAGCTAG TGCAATACAG CGCAAAGGCA GAGCGGGACG GTGTAGACCT 
GGAATTTGTT TCCGTCTGTT TAGTAGACTT CGATTTCAGA ACATGCTGGA ATTTCAGACG 
CCAGAACTCT TGAGAATGCC ATTGCAGGAA CTTTGTTTAC ATACCAAGCT GTTAGCCCCA 
GTTAACTGCA CCATTGCCGA CTTCCTTATG AAAGCCCCTG AACCGCCTCC AGCTTTAATT 
GTGAGAAATG CTGTGCAGAT GCTTAAGACA ATAGATGCAA TGGATGCATG GGAAGATCTG 
ACTGAACTTG GGTATCATTT GGCTGATTTG CCAGTAGAAC CGCATCTTGG GAAAATGGTC 
CTGTGTGCAG TGGTTTTGAA GTGTCTGGAC CCCATCCTTA CAATTGCCTG CACGCTCGCT 
TACCGTGACC CTTTTGTCCT GCCTACCCAG GCCTCTCAGA AACGGGCAGC TATGCTTTGT 
AGGAAACGCT TCACTGCAGG GACTTTCAGT GACCACATGG CACTTCTGAG AGCATTTCAG 
GCATGGCAGA AAGCACGAAG TGATGGGTGG GAGCGAGCCT TTTGTGAAAA GAATTTTCTT 
TCACAAGCTA CTATGGAAAT CATTATAGGC ATGAGAACAC AGTTGCTTGG TCAGCTTAGA 
GCATCAGGTT TTGTTAGAGC ACGAGGTGGT GGTGACATTC GAGATGTTAA TACAAACTCC 
GAGAACTGGG CTGTTGTTAA AGCTGCATTG GTAGCAGGCA TGTATCCTAA TTTAGTCCAC 
GTGGACAGAG AGAATGTAAT ATTGACAGGG CCAAAGGAAA AGAAAGTACG CTTTCATCCC 
ACTTCAGTTC TCAGTCAACC TCAATACAAA AAGATTCCTC CCGCCAATGG TCAGGCTGCA 
GCGATTCAGG CATTGCCCAC AGATTGGCTT ATTTATGATG AAATGACCAG AGCCCATCGA 
ATTGCTAATA TCAGATGTTG TTCAGCAGTG ACACCTGTTA CTGTCCTGGT GTTCTGTGGA 
CCAGCAAGAC TGGCAAGTAA CGCTCTTCAG GAACCTTCAT CCTTTAGAGC GGATGGTATT 
CCCAATGACA GCAGTGACAG TGAGATGGAA GACAGAACTA CTGCCAATTT GGCTGCTTTG 
AAGCTTGATG AATGGCTTAA CTTCAAACTA GAGCCAGAGG CTGCCAGTTT ATTGCTACAG 
CTCAGACAGA AGTGGCATAG TTTGTTTTTA CGCCGAATGA GAGCGCCATC TAAACCTTGG 
TCTCAAGTCG ATGAAGCTAC CATACGAGCA ATTATAGCTG TTTTAAGCAC TGAAGAACAG 
TCTGCAGGTT TACAGCAGCC ATCTGGAATT GGCCAGCGGC CAAGACCCAT GTCTTCAGAA 
GAACTTCCTT TGGCTTCATC TTGGAGATCC AATAATAGTA GGAAAAGTAC AGCTGACACT 
GAGTTTGCGG ATGGATCTAC TACTGGAGAA AGAGTACTGA TGAAATCTCC ATCTCCAGCC 
TTACATCCAC CTCAGAAGTA CAAAGATAGA GGAATTTTAC ATCCTAAGCG GAGCACCGAT 
GACCGGTCCG ATCAATCATC TGTGAAATCC ACAGACAGCA GCAGTTACCC AAGTCCTTGT 
GCCAGCCCCT CTCCTCCATC CTCAGGGAAG GGATCAAAAT CTCCTTCACC AAGACCAAAC 
ATGCCTATTC GTTACTTCAT AATGAAGAGT AGCAATCTGA GAAACCTTGA AATTTCTCAA 
CAGAAGGGTA TCTGGTCTAC AACCCCTAGT AATGAGCGGA AGCTAAATCG AGCGTTTTGG 
GAGAGCAGCA TGGTTTACTT GGTATTTTCT GTTCAAGGAT CTGGACATTT CCAGGGATTT 
TCTAGGATGT CTTCTGAGAT AGGAAGGGAG AAGAGCCAGG ACTGGGGGTC AGCTGGACTA 
GGTGGAGTAT TTAAAGTGGA GTGGATACGC AAAGAAAGCC TTCCCTTTCA GTTTGCACAC 
CATTTACTCA ATCCTTGGAA CGACAACAAG AAAGTACAGA TAAGCAGAGA TGGGCAGGAG 
CTAGAACCTC AGGTTGGGGA ACAGTTGCTC CAGTTATGGG AACGCCTTCC ATTGGGAGAA 
AAAACAACTT CTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

eLife7(2018 Jan)
Jain D,Puno MR,Meydan C,Lailler N,Mason CE,Lima CD,Anderson KV,Keeney S

The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline.
eLife6(2017 Oct)
Bailey AS,Batista PJ,Gold RS,Chen YG,de Rooij DG,Chang HY,Fuller MT

Regulation of m
Molecular cell68(2)374-387.e12(2017 Oct)
Wojtas MN,Pandey RR,Mendel M,Homolka D,Sachidanandam R,Pillai RS

Ythdc2 is an N
Cell research27(9)1115-1127(2017 Sep)
Hsu PJ,Zhu Y,Ma H,Guo Y,Shi X,Liu Y,Qi M,Lu Z,Shi H,Wang J,Cheng Y,Luo G,Dai Q,Liu M,Guo X,Sha J,Shen B,He C

Meioc maintains an extended meiotic prophase I in mice.
PLoS genetics13(4)e1006704(2017 Apr)
Soh YQS,Mikedis MM,Kojima M,Godfrey AK,de Rooij DG,Page DC

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Ythdc2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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