TMC3 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMC3
Entrez Gene ID	233424
Full Name	transmembrane channel-like gene family 3
Synonyms	9030203A06
General protein information	Preferred Names transmembrane channel-like gene family 3 Names transmembrane channel-like protein 3 tmc3
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 7 Map Location: 7\|7 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_177695.4	NP_808363.3	transmembrane channel-like protein 3
XM_006507591.3	XP_006507654.1	transmembrane channel-like protein 3 isoform X1
XM_006507591.2	XP_006507654.1	transmembrane channel-like protein 3 isoform X1
XM_011241734.1	XP_011240036.1	transmembrane channel-like protein 3 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tmc3	NP_001163903.1
Xenopus tropicalis (tropical clawed frog)	tmc3	XP_002932314.2
Gallus gallus (chicken)	TMC3	NP_001005820.1
Pan troglodytes (chimpanzee)	TMC3	XP_510545.2
	TMC3	XP_001110155.1
Bos taurus (cattle)	TMC3	XP_003587605.1
Mus musculus (house mouse)	Tmc3	NP_808363.3
Homo sapiens (human)	TMC3	NP_001074001.1
Canis lupus familiaris (dog)	TMC3	XP_545878.3

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Conductance and block of hair-cell mechanotransducer channels in transmembrane channel-like protein mutants.
Beurg M, Kim KX, Fettiplace R
The Journal of general physiology144(1)55-69(2014 Jul)

Characterization of the transmembrane channel-like (TMC) gene family: functional clues from hearing loss and epidermodysplasia verruciformis.
Kurima K, Yang Y, Sorber K, Griffith AJ
Genomics82(3)300-8(2003 Sep)

TMC and EVER genes belong to a larger novel family, the TMC gene family encoding transmembrane proteins.
Keresztes G, Mutai H, Heller S
BMC genomics4(1)24(2003 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TMC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu39457	XM_006507591.3 Latest version!	Mus musculus transmembrane channel-like gene family 3 (Tmc3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu06664	NM_177695.4 Latest version!	Mus musculus transmembrane channel-like gene family 3 (Tmc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu06664	XM_011241734.1 Latest version!	Mus musculus transmembrane channel-like gene family 3 (Tmc3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu39457	XM_006507591.2	Mus musculus transmembrane channel-like gene family 3 (Tmc3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMC3 ( XM_006507591.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006507654.1 Mus musculus transmembrane channel-like gene family 3 (Tmc3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu39457
Clone ID Related Accession (Same CDS sequence)	XM_006507591.3 , XM_006507591.2
Accession Version	XM_006507591.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transmembrane channel-like protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507591.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACCT CAAAGGCATC CCAGCGCTAC AGAAGCATTC GGAGAAACGC CAGCCAGTGT 
TATCTCTACC AGGACTCCCT GCTTCTTGGT AACTCCGATG ACAGCTTTAA TGCTGATGAA 
ACGGGAGACA GCAGTGATCC GGAACAAATC TTCCAGAATA TACAGTTTCA GAAAGATCTC 
ATGGCAAATA TCCGCTGCAG ACCCTGGACC ATGGGGCAGA AACTGCGGGC ACTGAGACGA 
GCCAAGGAGA TTGTGCTGAA GTTTGAAGGG AGGCTGACCA GGACTCGAGG CTACCAGGCA 
GCAGGAGCAG AGCTCTGGCG GAAATTCGCC CGTCTGGCCT GTAACTTTGT GGTCATCTTC 
ATCCCCTGGG AAATGAGGAT AAAGAAAATT GAGAGTCACT TCGGGTCTGG TGTGGCCTCC 
TACTTCATCT TCCTGAGGTG GCTATTTGGG ATTAACATAG TGCTCACAGT GATGACCGGT 
GCCTTTGTTG TCCTTCCCGA GCTGATCGCA GGCCAGCCCT TTGGAAGTAC AGCCAGCAAG 
ACCATTCCCC GGGAGCAGAT CACATCTGCA CAGGATCTGG ACACTGTCTG GTCCCTGGGG 
GGCTACCTTC AATACTCCGT CCTGTTCTAC GGGTACTACG GAAGAGAGAG GCGGATCGGG 
AGAGCCGGCT ACCGCCTGCC CTTGGCGTAT TTCCTGGTGG GGATGGCAGT GTTTGCTTAC 
AGCTTCATCG TTCTCTTAAA AAAGATGGCT AAGAACTCCC GCACCAGCCT AGCCAGTGCT 
TCCAATGAAA ATTACACCTT CTGCTGGCGG GTGTTTTGTG CCTGGGATTA TCTCATTGGG 
AACCCAGAGG CTGCAGAAAG CAAAACAGCT GCCATCTTGA ACAGCATTAG GGAGGCCATA 
CTGGAAGAGC AAGAAAAGAA AAAAAACAAA AACATGGCGG TGACTGTCTG TCTGAGAATC 
ATTGCCAACA TCCTGGTGCT CCTCTCACTG GCTGGAAGCA TCTATCTCAT CTACTTTGTG 
GTGGACCGGT CCCAGAAGCT GGAGCAGTCG AAGAAGGAGC TGACACTTTG GGAAAAGAAT 
GAGGTAAGCG TGGTGGTCTC CCTCGTCACC ATGCTAGCAC CGTCTGCCTT TGACCTCATC 
GCTGCCTTGG AGATGTATCA TCCACGGACG ACACTGCGCT TCCAGTTAGC AAGGGTCCTG 
GTACTGTATT TAGGAAATCT CTACAGCTTG ATCATTGCTC TTCTGGACAA AGTGAACAGC 
ATGAACATTG AGGAAGCTGC TACTAAAAAC ATAACAAGCC ACTGGGCAGA TGCTCCCACC 
TTCTCTGCCA CCAGGACAGT GCCTGAAGAA GGGCAGTGGC CCACACCTGG ATCAGGAGCG 
GAGCTCAGGA GAAACACTAG CACATGGGTC GTAGAAGAGA CAAGCTTTCT GACTTCTATC 
ACGCCACATA CAAAGGCCAA CAAGACAGTG CCCTACATGC AGGGCCCACA AGGCCAGTGC 
TGGGAGACAT ATGTCGGCCA GGAGATGCTG AAGCTGTCTG TCATCGACAT GCTCTTCACT 
GTGGCCAGCA TCCTCCTCAT AGACTTCTTC CGAGGACTTT TTGTTCGCTA CCTAAGTGAC 
TATTGGTGTT GGGACCTTGA AAGCAAGTTT CCTGAATATG GAGAATTCAA GATTGCCGAG 
AATGTGCTGC ACCTTGTCTA CAATCAAGGC ATGATTTGGA TGGGGGCCTT CTTCTCTCCG 
TGTCTGCCAG CATTCAATGT TCTTAAGCTC ATTGGGCTGA TGTACCTGAG GAGCTGGGCG 
GTGCTGACCT GCAATGTACC CCACCAGCAA GTGTTTCGAG CTTCCAGATC CAACAACTTC 
TACCTGGCAA TGCTCCTGTT CATGTTGTTC CTTTGCATGC TGCCCACCAT TTTTGCCATT 
GTCCACTACA AGCCGTCTCT GAACTGTGGT CCCTTCAGCG GACAAGAGAA AATTTATGAC 
ATCGTGTCCG AGACGATAGA GAATGACTTC CCGACCTGGT TTCACGCTGT AGTTGGACAT 
ATCAGCAGCC CCGTGGTGAT ACTTCCAGCT GTGCTGCTTC TGTTCATGCT CATCTACTAC 
CTTCAGAGCA TCGCGAGATC ACTCAAGCTC AGCAGCCAGC AACTCAGGAT GCAGATCCAA 
AATGCGAGAT CTGAAGATAA GAAAAAGGTT GCCCAAATGG TAGAGGCTCG AATCCAGACT 
CATGAGGAAA GCAGCAAGAG GCTTCTAAAG GACAGCGACC TTATCAGCCA GCTGTCCTCA 
GTGTATATGG CAACATCCCC AAACAATGGC CATATGCTTA ACTTCGACTC TCTGAGTAGC 
AAGAGCCTCA GGATGGAGGC AATCACAAGG TCCCTGCCAC AGAGTCCTGG ACAAGGGTCC 
AGGGACCCCT GCTCTCCTCT TCTAGATGGA TCTAGGTCAA GGCCAGAGCA GGACACCAAC 
AGGCATCCAC ATAGGCCATG TTCCAGCACA AGCAACCTCC ACAAGAACAG ATCCTGCTCA 
TCTGTGACAC AGACACAGCC CCTCAAAGAT GTCCGCTCAG AGCCTCTTTC CCGGAAAGAC 
TTTCAGCCAA TCAGTCCTCC TTTCTGTGGA TCTGGGGTCT CTACCTTGAT GACACATGAC 
CACAGTCCCC GTGCTCCCAG GTACTATGTG GTTAATGAAC GTGACTCTCA CAAAAAGACC 
CACCGAGCTT TCTGGCCAGA AAGGCATTTC AAGATAGATG CTTTAGGTGA CATTGTAGAG 
CTGTACCCCA GGAATGTGCA GCAGTACATG TCCTGGGTTC CCAACCAGCC TTGCTCTCCA 
CAGCTGAGTG AAGAAGAGGA GGAGATGCTC AGGAGAGATT TGGTCCAGTG GTCAATCCCT 
GCTAGCTCCC TGACAGACCT GCCTCGGTCT TCTTGTTTTT ACACTGGTGA CAGATCAGAA 
AATAACACCA GAGACCCTAA ATACCAGCGA AGGGTGTACT ACAGATCTGG GGATAATAGT 
TTTGAAGACC AGCTTGAGAG GCCCACATTT GTACACAGAA AGCCACGATC CAGGAACGGC 
CAATACCCAC AGCATGCCCT GAAGGCCAGA GTCAAGGCCA AGTTCGAGCC GTCCTTCACA 
GAATCTGATT CTGTGTCAGC AGCATCCAGC AGTGACCATC AAAACAGCAA CAATGACCAA 
TACCTGCATG TCATGTCCAG CCAGGGCAGA TTCCCAAGGT CTGCCAGCCA GCTGGGCAGG 
AGGAAGGCCA AGTCCAGGCA GGTACTACCC ACAGATCTAA ATGATCTAAT TTGTTCAAAT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507654.1
CDS	188..3493
Translation	MKTSKASQRYRSIRRNASQCYLYQDSLLLGNSDDSFNADETGDSSDPEQIFQNIQFQKDLMANIRCRPWTMGQKLRALRRAKEIVLKFEGRLTRTRGYQAAGAELWRKFARLACNFVVIFIPWEMRIKKIESHFGSGVASYFIFLRWLFGINIVLTVMTGAFVVLPELIAGQPFGSTASKTIPREQITSAQDLDTVWSLGGYLQYSVLFYGYYGRERRIGRAGYRLPLAYFLVGMAVFAYSFIVLLKKMAKNSRTSLASASNENYTFCWRVFCAWDYLIGNPEAAESKTAAILNSIREAILEEQEKKKNKNMAVTVCLRIIANILVLLSLAGSIYLIYFVVDRSQKLEQSKKELTLWEKNEVSVVVSLVTMLAPSAFDLIAALEMYHPRTTLRFQLARVLVLYLGNLYSLIIALLDKVNSMNIEEAATKNITSHWADAPTFSATRTVPEEGQWPTPGSGAELRRNTSTWVVEETSFLTSITPHTKANKTVPYMQGPQGQCWETYVGQEMLKLSVIDMLFTVASILLIDFFRGLFVRYLSDYWCWDLESKFPEYGEFKIAENVLHLVYNQGMIWMGAFFSPCLPAFNVLKLIGLMYLRSWAVLTCNVPHQQVFRASRSNNFYLAMLLFMLFLCMLPTIFAIVHYKPSLNCGPFSGQEKIYDIVSETIENDFPTWFHAVVGHISSPVVILPAVLLLFMLIYYLQSIARSLKLSSQQLRMQIQNARSEDKKKVAQMVEARIQTHEESSKRLLKDSDLISQLSSVYMATSPNNGHMLNFDSLSSKSLRMEAITRSLPQSPGQGSRDPCSPLLDGSRSRPEQDTNRHPHRPCSSTSNLHKNRSCSSVTQTQPLKDVRSEPLSRKDFQPISPPFCGSGVSTLMTHDHSPRAPRYYVVNERDSHKKTHRAFWPERHFKIDALGDIVELYPRNVQQYMSWVPNQPCSPQLSEEEEEMLRRDLVQWSIPASSLTDLPRSSCFYTGDRSENNTRDPKYQRRVYYRSGDNSFEDQLERPTFVHRKPRSRNGQYPQHALKARVKAKFEPSFTESDSVSAASSSDHQNSNNDQYLHVMSSQGRFPRSASQLGRRKAKSRQVLPTDLNDLICSNV

Target ORF information:

RefSeq Version	XM_006507591.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane channel-like gene family 3 (Tmc3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507591.3

ORF Insert Sequence:

ATGAAAACCT CAAAGGCATC CCAGCGCTAC AGAAGCATTC GGAGAAACGC CAGCCAGTGT 
TATCTCTACC AGGACTCCCT GCTTCTTGGT AACTCCGATG ACAGCTTTAA TGCTGATGAA 
ACGGGAGACA GCAGTGATCC GGAACAAATC TTCCAGAATA TACAGTTTCA GAAAGATCTC 
ATGGCAAATA TCCGCTGCAG ACCCTGGACC ATGGGGCAGA AACTGCGGGC ACTGAGACGA 
GCCAAGGAGA TTGTGCTGAA GTTTGAAGGG AGGCTGACCA GGACTCGAGG CTACCAGGCA 
GCAGGAGCAG AGCTCTGGCG GAAATTCGCC CGTCTGGCCT GTAACTTTGT GGTCATCTTC 
ATCCCCTGGG AAATGAGGAT AAAGAAAATT GAGAGTCACT TCGGGTCTGG TGTGGCCTCC 
TACTTCATCT TCCTGAGGTG GCTATTTGGG ATTAACATAG TGCTCACAGT GATGACCGGT 
GCCTTTGTTG TCCTTCCCGA GCTGATCGCA GGCCAGCCCT TTGGAAGTAC AGCCAGCAAG 
ACCATTCCCC GGGAGCAGAT CACATCTGCA CAGGATCTGG ACACTGTCTG GTCCCTGGGG 
GGCTACCTTC AATACTCCGT CCTGTTCTAC GGGTACTACG GAAGAGAGAG GCGGATCGGG 
AGAGCCGGCT ACCGCCTGCC CTTGGCGTAT TTCCTGGTGG GGATGGCAGT GTTTGCTTAC 
AGCTTCATCG TTCTCTTAAA AAAGATGGCT AAGAACTCCC GCACCAGCCT AGCCAGTGCT 
TCCAATGAAA ATTACACCTT CTGCTGGCGG GTGTTTTGTG CCTGGGATTA TCTCATTGGG 
AACCCAGAGG CTGCAGAAAG CAAAACAGCT GCCATCTTGA ACAGCATTAG GGAGGCCATA 
CTGGAAGAGC AAGAAAAGAA AAAAAACAAA AACATGGCGG TGACTGTCTG TCTGAGAATC 
ATTGCCAACA TCCTGGTGCT CCTCTCACTG GCTGGAAGCA TCTATCTCAT CTACTTTGTG 
GTGGACCGGT CCCAGAAGCT GGAGCAGTCG AAGAAGGAGC TGACACTTTG GGAAAAGAAT 
GAGGTAAGCG TGGTGGTCTC CCTCGTCACC ATGCTAGCAC CGTCTGCCTT TGACCTCATC 
GCTGCCTTGG AGATGTATCA TCCACGGACG ACACTGCGCT TCCAGTTAGC AAGGGTCCTG 
GTACTGTATT TAGGAAATCT CTACAGCTTG ATCATTGCTC TTCTGGACAA AGTGAACAGC 
ATGAACATTG AGGAAGCTGC TACTAAAAAC ATAACAAGCC ACTGGGCAGA TGCTCCCACC 
TTCTCTGCCA CCAGGACAGT GCCTGAAGAA GGGCAGTGGC CCACACCTGG ATCAGGAGCG 
GAGCTCAGGA GAAACACTAG CACATGGGTC GTAGAAGAGA CAAGCTTTCT GACTTCTATC 
ACGCCACATA CAAAGGCCAA CAAGACAGTG CCCTACATGC AGGGCCCACA AGGCCAGTGC 
TGGGAGACAT ATGTCGGCCA GGAGATGCTG AAGCTGTCTG TCATCGACAT GCTCTTCACT 
GTGGCCAGCA TCCTCCTCAT AGACTTCTTC CGAGGACTTT TTGTTCGCTA CCTAAGTGAC 
TATTGGTGTT GGGACCTTGA AAGCAAGTTT CCTGAATATG GAGAATTCAA GATTGCCGAG 
AATGTGCTGC ACCTTGTCTA CAATCAAGGC ATGATTTGGA TGGGGGCCTT CTTCTCTCCG 
TGTCTGCCAG CATTCAATGT TCTTAAGCTC ATTGGGCTGA TGTACCTGAG GAGCTGGGCG 
GTGCTGACCT GCAATGTACC CCACCAGCAA GTGTTTCGAG CTTCCAGATC CAACAACTTC 
TACCTGGCAA TGCTCCTGTT CATGTTGTTC CTTTGCATGC TGCCCACCAT TTTTGCCATT 
GTCCACTACA AGCCGTCTCT GAACTGTGGT CCCTTCAGCG GACAAGAGAA AATTTATGAC 
ATCGTGTCCG AGACGATAGA GAATGACTTC CCGACCTGGT TTCACGCTGT AGTTGGACAT 
ATCAGCAGCC CCGTGGTGAT ACTTCCAGCT GTGCTGCTTC TGTTCATGCT CATCTACTAC 
CTTCAGAGCA TCGCGAGATC ACTCAAGCTC AGCAGCCAGC AACTCAGGAT GCAGATCCAA 
AATGCGAGAT CTGAAGATAA GAAAAAGGTT GCCCAAATGG TAGAGGCTCG AATCCAGACT 
CATGAGGAAA GCAGCAAGAG GCTTCTAAAG GACAGCGACC TTATCAGCCA GCTGTCCTCA 
GTGTATATGG CAACATCCCC AAACAATGGC CATATGCTTA ACTTCGACTC TCTGAGTAGC 
AAGAGCCTCA GGATGGAGGC AATCACAAGG TCCCTGCCAC AGAGTCCTGG ACAAGGGTCC 
AGGGACCCCT GCTCTCCTCT TCTAGATGGA TCTAGGTCAA GGCCAGAGCA GGACACCAAC 
AGGCATCCAC ATAGGCCATG TTCCAGCACA AGCAACCTCC ACAAGAACAG ATCCTGCTCA 
TCTGTGACAC AGACACAGCC CCTCAAAGAT GTCCGCTCAG AGCCTCTTTC CCGGAAAGAC 
TTTCAGCCAA TCAGTCCTCC TTTCTGTGGA TCTGGGGTCT CTACCTTGAT GACACATGAC 
CACAGTCCCC GTGCTCCCAG GTACTATGTG GTTAATGAAC GTGACTCTCA CAAAAAGACC 
CACCGAGCTT TCTGGCCAGA AAGGCATTTC AAGATAGATG CTTTAGGTGA CATTGTAGAG 
CTGTACCCCA GGAATGTGCA GCAGTACATG TCCTGGGTTC CCAACCAGCC TTGCTCTCCA 
CAGCTGAGTG AAGAAGAGGA GGAGATGCTC AGGAGAGATT TGGTCCAGTG GTCAATCCCT 
GCTAGCTCCC TGACAGACCT GCCTCGGTCT TCTTGTTTTT ACACTGGTGA CAGATCAGAA 
AATAACACCA GAGACCCTAA ATACCAGCGA AGGGTGTACT ACAGATCTGG GGATAATAGT 
TTTGAAGACC AGCTTGAGAG GCCCACATTT GTACACAGAA AGCCACGATC CAGGAACGGC 
CAATACCCAC AGCATGCCCT GAAGGCCAGA GTCAAGGCCA AGTTCGAGCC GTCCTTCACA 
GAATCTGATT CTGTGTCAGC AGCATCCAGC AGTGACCATC AAAACAGCAA CAATGACCAA 
TACCTGCATG TCATGTCCAG CCAGGGCAGA TTCCCAAGGT CTGCCAGCCA GCTGGGCAGG 
AGGAAGGCCA AGTCCAGGCA GGTACTACCC ACAGATCTAA ATGATCTAAT TTGTTCAAAT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMC3 ( NM_177695.4 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_808363.3 Mus musculus transmembrane channel-like gene family 3 (Tmc3), mRNA.

Price & Availability↑

CloneID	OMu06664
Clone ID Related Accession (Same CDS sequence)	NM_177695.4 , XM_011241734.1
Accession Version	NM_177695.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	transmembrane channel-like protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY236491.1, AY263157.1 and AC158576.3. On Jul 25, 2006 this sequence version replaced NM_177695.3. ##Evidence-Data-START## Transcript exon combination :: AY263157.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01164137, SAMN01164142 [ECO:0000350] ##Evidence-Data-END##

ATGAAAACCT CAAAGGCATC CCAGCGCTAC AGAAGCATTC GGAGAAACGC CAGCCAGTGT 
TATCTCTACC AGGACTCCCT GCTTCTTGGT AACTCCGATG ACAGCTTTAA TGCTGATGAA 
ACGGGAGACA GCAGTGATCC GGAACAAATC TTCCAGAATA TACAGTTTCA GAAAGATCTC 
ATGGCAAATA TCCGCTGCAG ACCCTGGACC ATGGGGCAGA AACTGCGGGC ACTGAGACGA 
GCCAAGGAGA TTGTGCTGAA GTTTGAAGGG AGGCTGACCA GGACTCGAGG CTACCAGGCA 
GCAGGAGCAG AGCTCTGGCG GAAATTCGCC CGTCTGGCCT GTAACTTTGT GGTCATCTTC 
ATCCCCTGGG AAATGAGGAT AAAGAAAATT GAGAGTCACT TCGGGTCTGG TGTGGCCTCC 
TACTTCATCT TCCTGAGGTG GCTATTTGGG ATTAACATAG TGCTCACAGT GATGACCGGT 
GCCTTTGTTG TCCTTCCCGA GCTGATCGCA GGCCAGCCCT TTGGAAGTAC AGCCAGCAAG 
ACCATTCCCC GGGAGCAGAT CACATCTGCA CAGGATCTGG ACACTGTCTG GTCCCTGGGG 
GGCTACCTTC AATACTCCGT CCTGTTCTAC GGGTACTACG GAAGAGAGAG GCGGATCGGG 
AGAGCCGGCT ACCGCCTGCC CTTGGCGTAT TTCCTGGTGG GGATGGCAGT GTTTGCTTAC 
AGCTTCATCG TTCTCTTAAA AAAGATGGCT AAGAACTCCC GCACCAGCCT AGCCAGTGCT 
TCCAATGAAA ATTACACCTT CTGCTGGCGG GTGTTTTGTG CCTGGGATTA TCTCATTGGG 
AACCCAGAGG CTGCAGAAAG CAAAACAGCT GCCATCTTGA ACAGCATTAG GGAGGCCATA 
CTGGAAGAGC AAGAAAAGAA AAAAAACAAA AACATGGCGG TGACTGTCTG TCTGAGAATC 
ATTGCCAACA TCCTGGTGCT CCTCTCACTG GCTGGAAGCA TCTATCTCAT CTACTTTGTG 
GTGGACCGGT CCCAGAAGCT GGAGCAGTCG AAGAAGGAGC TGACACTTTG GGAAAAGAAT 
GAGGTAAGCG TGGTGGTCTC CCTCGTCACC ATGCTAGCAC CGTCTGCCTT TGACCTCATC 
GCTGCCTTGG AGATGTATCA TCCACGGACG ACACTGCGCT TCCAGTTAGC AAGGGTCCTG 
GTACTGTATT TAGGAAATCT CTACAGCTTG ATCATTGCTC TTCTGGACAA AGTGAACAGC 
ATGAACATTG AGGAAGCTGC TACTAAAAAC ATAACAAGCC ACTGGGCAGA TGCTCCCACC 
TTCTCTGCCA CCAGGACAGT GCCTGAAGAA GGGCAGTGGC CCACACCTGG ATCAGGAGCG 
GAGCTCAGGA GAAACACTAG CACATGGGTC GTAGAAGAGA CAAGCTTTCT GACTTCTATC 
ACGCCACATA CAAAGGCCAA CAAGACAGTG CCCTACATGC AGGGCCCACA AGGCCAGTGC 
TGGGAGACAT ATGTCGGCCA GGAGATGCTG AAGCTGTCTG TCATCGACAT GCTCTTCACT 
GTGGCCAGCA TCCTCCTCAT AGACTTCTTC CGAGGACTTT TTGTTCGCTA CCTAAGTGAC 
TATTGGTGTT GGGACCTTGA AAGCAAGTTT CCTGAATATG GAGAATTCAA GATTGCCGAG 
AATGTGCTGC ACCTTGTCTA CAATCAAGGC ATGATTTGGA TGGGGGCCTT CTTCTCTCCG 
TGTCTGCCAG CATTCAATGT TCTTAAGCTC ATTGGGCTGA TGTACCTGAG GAGCTGGGCG 
GTGCTGACCT GCAATGTACC CCACCAGCAA GTGTTTCGAG CTTCCAGATC CAACAACTTC 
TACCTGGCAA TGCTCCTGTT CATGTTGTTC CTTTGCATGC TGCCCACCAT TTTTGCCATT 
GTCCACTACA AGCCGTCTCT GAACTGTGGT CCCTTCAGCG GACAAGAGAA AATTTATGAC 
ATCGTGTCCG AGACGATAGA GAATGACTTC CCGACCTGGT TTCACGCTGT AGTTGGACAT 
ATCAGCAGCC CCGTGGTGAT ACTTCCAGCT GTGCTGCTTC TGTTCATGCT CATCTACTAC 
CTTCAGAGCA TCGCGAGATC ACTCAAGCTC AGCAGCCAGC AACTCAGGAT GCAGATCCAA 
AATGCGAGAT CTGAAGATAA GAAAAAGGTT GCCCAAATGG TAGAGGCCCT AGCCATCCCT 
AGCGATGCCA GGCAGGCAGG CTCAGCCACT GAAGCAGAGT CGAGTGAAAA TTCAAAGCCA 
AAGACTCTCC AAGCTCGAAT CCAGACTCAT GAGGAAAGCA GCAAGAGGCT TCTAAAGGAC 
AGCGACCTTA TCAGCCAGCT GTCCTCAGTG TATATGGCAA CATCCCCAAA CAATGGCCAT 
ATGCTTAACT TCGACTCTCT GAGTAGCAAG AGCCTCAGGA TGGAGGCAAT CACAAGGTCC 
CTGCCACAGA GTCCTGGACA AGGGTCCAGG GACCCCTGCT CTCCTCTTCT AGATGGATCT 
AGGTCAAGGC CAGAGCAGGA CACCAACAGG CATCCACATA GGCCATGTTC CAGCACAAGC 
AACCTCCACA AGAACAGATC CTGCTCATCT GTGACACAGA CACAGCCCCT CAAAGATGTC 
CGCTCAGAGC CTCTTTCCCG GAAAGACTTT CAGCCAATCA GTCCTCCTTT CTGTGGATCT 
GGGGTCTCTA CCTTGATGAC ACATGACCAC AGTCCCCGTG CTCCCAGGTA CTATGTGGTT 
AATGAACGTG ACTCTCACAA AAAGACCCAC CGAGCTTTCT GGCCAGAAAG GCATTTCAAG 
ATAGATGCTT TAGGTGACAT TGTAGAGCTG TACCCCAGGA ATGTGCAGCA GTACATGTCC 
TGGGTTCCCA ACCAGCCTTG CTCTCCACAG CTGAGTGAAG AAGAGGAGGA GATGCTCAGG 
AGAGATTTGG TCCAGTGGTC AATCCCTGCT AGCTCCCTGA CAGACCTGCC TCGGTCTTCT 
TGTTTTTACA CTGGTGACAG ATCAGAAAAT AACACCAGAG ACCCTAAATA CCAGCGAAGG 
GTGTACTACA GATCTGGGGA TAATAGTTTT GAAGACCAGC TTGAGAGGCC CACATTTGTA 
CACAGAAAGC CACGATCCAG GAACGGCCAA TACCCACAGC ATGCCCTGAA GGCCAGAGTC 
AAGGCCAAGT TCGAGCCGTC CTTCACAGAA TCTGATTCTG TGTCAGCAGC ATCCAGCAGT 
GACCATCAAA ACAGCAACAA TGACCAATAC CTGCATGTCA TGTCCAGCCA GGGCAGATTC 
CCAAGGTCTG CCAGCCAGCT GGGCAGGAGG AAGGCCAAGT CCAGGCAGGT ACTACCCACA 
GATCTAAATG ATCTAATTTG TTCAAATGTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808363.3
CDS	162..3554
Translation	MKTSKASQRYRSIRRNASQCYLYQDSLLLGNSDDSFNADETGDSSDPEQIFQNIQFQKDLMANIRCRPWTMGQKLRALRRAKEIVLKFEGRLTRTRGYQAAGAELWRKFARLACNFVVIFIPWEMRIKKIESHFGSGVASYFIFLRWLFGINIVLTVMTGAFVVLPELIAGQPFGSTASKTIPREQITSAQDLDTVWSLGGYLQYSVLFYGYYGRERRIGRAGYRLPLAYFLVGMAVFAYSFIVLLKKMAKNSRTSLASASNENYTFCWRVFCAWDYLIGNPEAAESKTAAILNSIREAILEEQEKKKNKNMAVTVCLRIIANILVLLSLAGSIYLIYFVVDRSQKLEQSKKELTLWEKNEVSVVVSLVTMLAPSAFDLIAALEMYHPRTTLRFQLARVLVLYLGNLYSLIIALLDKVNSMNIEEAATKNITSHWADAPTFSATRTVPEEGQWPTPGSGAELRRNTSTWVVEETSFLTSITPHTKANKTVPYMQGPQGQCWETYVGQEMLKLSVIDMLFTVASILLIDFFRGLFVRYLSDYWCWDLESKFPEYGEFKIAENVLHLVYNQGMIWMGAFFSPCLPAFNVLKLIGLMYLRSWAVLTCNVPHQQVFRASRSNNFYLAMLLFMLFLCMLPTIFAIVHYKPSLNCGPFSGQEKIYDIVSETIENDFPTWFHAVVGHISSPVVILPAVLLLFMLIYYLQSIARSLKLSSQQLRMQIQNARSEDKKKVAQMVEALAIPSDARQAGSATEAESSENSKPKTLQARIQTHEESSKRLLKDSDLISQLSSVYMATSPNNGHMLNFDSLSSKSLRMEAITRSLPQSPGQGSRDPCSPLLDGSRSRPEQDTNRHPHRPCSSTSNLHKNRSCSSVTQTQPLKDVRSEPLSRKDFQPISPPFCGSGVSTLMTHDHSPRAPRYYVVNERDSHKKTHRAFWPERHFKIDALGDIVELYPRNVQQYMSWVPNQPCSPQLSEEEEEMLRRDLVQWSIPASSLTDLPRSSCFYTGDRSENNTRDPKYQRRVYYRSGDNSFEDQLERPTFVHRKPRSRNGQYPQHALKARVKAKFEPSFTESDSVSAASSSDHQNSNNDQYLHVMSSQGRFPRSASQLGRRKAKSRQVLPTDLNDLICSNV

Target ORF information:

RefSeq Version	NM_177695.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane channel-like gene family 3 (Tmc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177695.4

ORF Insert Sequence:

ATGAAAACCT CAAAGGCATC CCAGCGCTAC AGAAGCATTC GGAGAAACGC CAGCCAGTGT 
TATCTCTACC AGGACTCCCT GCTTCTTGGT AACTCCGATG ACAGCTTTAA TGCTGATGAA 
ACGGGAGACA GCAGTGATCC GGAACAAATC TTCCAGAATA TACAGTTTCA GAAAGATCTC 
ATGGCAAATA TCCGCTGCAG ACCCTGGACC ATGGGGCAGA AACTGCGGGC ACTGAGACGA 
GCCAAGGAGA TTGTGCTGAA GTTTGAAGGG AGGCTGACCA GGACTCGAGG CTACCAGGCA 
GCAGGAGCAG AGCTCTGGCG GAAATTCGCC CGTCTGGCCT GTAACTTTGT GGTCATCTTC 
ATCCCCTGGG AAATGAGGAT AAAGAAAATT GAGAGTCACT TCGGGTCTGG TGTGGCCTCC 
TACTTCATCT TCCTGAGGTG GCTATTTGGG ATTAACATAG TGCTCACAGT GATGACCGGT 
GCCTTTGTTG TCCTTCCCGA GCTGATCGCA GGCCAGCCCT TTGGAAGTAC AGCCAGCAAG 
ACCATTCCCC GGGAGCAGAT CACATCTGCA CAGGATCTGG ACACTGTCTG GTCCCTGGGG 
GGCTACCTTC AATACTCCGT CCTGTTCTAC GGGTACTACG GAAGAGAGAG GCGGATCGGG 
AGAGCCGGCT ACCGCCTGCC CTTGGCGTAT TTCCTGGTGG GGATGGCAGT GTTTGCTTAC 
AGCTTCATCG TTCTCTTAAA AAAGATGGCT AAGAACTCCC GCACCAGCCT AGCCAGTGCT 
TCCAATGAAA ATTACACCTT CTGCTGGCGG GTGTTTTGTG CCTGGGATTA TCTCATTGGG 
AACCCAGAGG CTGCAGAAAG CAAAACAGCT GCCATCTTGA ACAGCATTAG GGAGGCCATA 
CTGGAAGAGC AAGAAAAGAA AAAAAACAAA AACATGGCGG TGACTGTCTG TCTGAGAATC 
ATTGCCAACA TCCTGGTGCT CCTCTCACTG GCTGGAAGCA TCTATCTCAT CTACTTTGTG 
GTGGACCGGT CCCAGAAGCT GGAGCAGTCG AAGAAGGAGC TGACACTTTG GGAAAAGAAT 
GAGGTAAGCG TGGTGGTCTC CCTCGTCACC ATGCTAGCAC CGTCTGCCTT TGACCTCATC 
GCTGCCTTGG AGATGTATCA TCCACGGACG ACACTGCGCT TCCAGTTAGC AAGGGTCCTG 
GTACTGTATT TAGGAAATCT CTACAGCTTG ATCATTGCTC TTCTGGACAA AGTGAACAGC 
ATGAACATTG AGGAAGCTGC TACTAAAAAC ATAACAAGCC ACTGGGCAGA TGCTCCCACC 
TTCTCTGCCA CCAGGACAGT GCCTGAAGAA GGGCAGTGGC CCACACCTGG ATCAGGAGCG 
GAGCTCAGGA GAAACACTAG CACATGGGTC GTAGAAGAGA CAAGCTTTCT GACTTCTATC 
ACGCCACATA CAAAGGCCAA CAAGACAGTG CCCTACATGC AGGGCCCACA AGGCCAGTGC 
TGGGAGACAT ATGTCGGCCA GGAGATGCTG AAGCTGTCTG TCATCGACAT GCTCTTCACT 
GTGGCCAGCA TCCTCCTCAT AGACTTCTTC CGAGGACTTT TTGTTCGCTA CCTAAGTGAC 
TATTGGTGTT GGGACCTTGA AAGCAAGTTT CCTGAATATG GAGAATTCAA GATTGCCGAG 
AATGTGCTGC ACCTTGTCTA CAATCAAGGC ATGATTTGGA TGGGGGCCTT CTTCTCTCCG 
TGTCTGCCAG CATTCAATGT TCTTAAGCTC ATTGGGCTGA TGTACCTGAG GAGCTGGGCG 
GTGCTGACCT GCAATGTACC CCACCAGCAA GTGTTTCGAG CTTCCAGATC CAACAACTTC 
TACCTGGCAA TGCTCCTGTT CATGTTGTTC CTTTGCATGC TGCCCACCAT TTTTGCCATT 
GTCCACTACA AGCCGTCTCT GAACTGTGGT CCCTTCAGCG GACAAGAGAA AATTTATGAC 
ATCGTGTCCG AGACGATAGA GAATGACTTC CCGACCTGGT TTCACGCTGT AGTTGGACAT 
ATCAGCAGCC CCGTGGTGAT ACTTCCAGCT GTGCTGCTTC TGTTCATGCT CATCTACTAC 
CTTCAGAGCA TCGCGAGATC ACTCAAGCTC AGCAGCCAGC AACTCAGGAT GCAGATCCAA 
AATGCGAGAT CTGAAGATAA GAAAAAGGTT GCCCAAATGG TAGAGGCCCT AGCCATCCCT 
AGCGATGCCA GGCAGGCAGG CTCAGCCACT GAAGCAGAGT CGAGTGAAAA TTCAAAGCCA 
AAGACTCTCC AAGCTCGAAT CCAGACTCAT GAGGAAAGCA GCAAGAGGCT TCTAAAGGAC 
AGCGACCTTA TCAGCCAGCT GTCCTCAGTG TATATGGCAA CATCCCCAAA CAATGGCCAT 
ATGCTTAACT TCGACTCTCT GAGTAGCAAG AGCCTCAGGA TGGAGGCAAT CACAAGGTCC 
CTGCCACAGA GTCCTGGACA AGGGTCCAGG GACCCCTGCT CTCCTCTTCT AGATGGATCT 
AGGTCAAGGC CAGAGCAGGA CACCAACAGG CATCCACATA GGCCATGTTC CAGCACAAGC 
AACCTCCACA AGAACAGATC CTGCTCATCT GTGACACAGA CACAGCCCCT CAAAGATGTC 
CGCTCAGAGC CTCTTTCCCG GAAAGACTTT CAGCCAATCA GTCCTCCTTT CTGTGGATCT 
GGGGTCTCTA CCTTGATGAC ACATGACCAC AGTCCCCGTG CTCCCAGGTA CTATGTGGTT 
AATGAACGTG ACTCTCACAA AAAGACCCAC CGAGCTTTCT GGCCAGAAAG GCATTTCAAG 
ATAGATGCTT TAGGTGACAT TGTAGAGCTG TACCCCAGGA ATGTGCAGCA GTACATGTCC 
TGGGTTCCCA ACCAGCCTTG CTCTCCACAG CTGAGTGAAG AAGAGGAGGA GATGCTCAGG 
AGAGATTTGG TCCAGTGGTC AATCCCTGCT AGCTCCCTGA CAGACCTGCC TCGGTCTTCT 
TGTTTTTACA CTGGTGACAG ATCAGAAAAT AACACCAGAG ACCCTAAATA CCAGCGAAGG 
GTGTACTACA GATCTGGGGA TAATAGTTTT GAAGACCAGC TTGAGAGGCC CACATTTGTA 
CACAGAAAGC CACGATCCAG GAACGGCCAA TACCCACAGC ATGCCCTGAA GGCCAGAGTC 
AAGGCCAAGT TCGAGCCGTC CTTCACAGAA TCTGATTCTG TGTCAGCAGC ATCCAGCAGT 
GACCATCAAA ACAGCAACAA TGACCAATAC CTGCATGTCA TGTCCAGCCA GGGCAGATTC 
CCAAGGTCTG CCAGCCAGCT GGGCAGGAGG AAGGCCAAGT CCAGGCAGGT ACTACCCACA 
GATCTAAATG ATCTAATTTG TTCAAATGTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMC3 ( XM_011241734.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011240036.1 Mus musculus transmembrane channel-like gene family 3 (Tmc3), transcript variant X1, mRNA.

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CloneID	OMu06664
Clone ID Related Accession (Same CDS sequence)	NM_177695.4 , XM_011241734.1
Accession Version	XM_011241734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	transmembrane channel-like protein 3 isoform X1
Comment

ATGAAAACCT CAAAGGCATC CCAGCGCTAC AGAAGCATTC GGAGAAACGC CAGCCAGTGT 
TATCTCTACC AGGACTCCCT GCTTCTTGGT AACTCCGATG ACAGCTTTAA TGCTGATGAA 
ACGGGAGACA GCAGTGATCC GGAACAAATC TTCCAGAATA TACAGTTTCA GAAAGATCTC 
ATGGCAAATA TCCGCTGCAG ACCCTGGACC ATGGGGCAGA AACTGCGGGC ACTGAGACGA 
GCCAAGGAGA TTGTGCTGAA GTTTGAAGGG AGGCTGACCA GGACTCGAGG CTACCAGGCA 
GCAGGAGCAG AGCTCTGGCG GAAATTCGCC CGTCTGGCCT GTAACTTTGT GGTCATCTTC 
ATCCCCTGGG AAATGAGGAT AAAGAAAATT GAGAGTCACT TCGGGTCTGG TGTGGCCTCC 
TACTTCATCT TCCTGAGGTG GCTATTTGGG ATTAACATAG TGCTCACAGT GATGACCGGT 
GCCTTTGTTG TCCTTCCCGA GCTGATCGCA GGCCAGCCCT TTGGAAGTAC AGCCAGCAAG 
ACCATTCCCC GGGAGCAGAT CACATCTGCA CAGGATCTGG ACACTGTCTG GTCCCTGGGG 
GGCTACCTTC AATACTCCGT CCTGTTCTAC GGGTACTACG GAAGAGAGAG GCGGATCGGG 
AGAGCCGGCT ACCGCCTGCC CTTGGCGTAT TTCCTGGTGG GGATGGCAGT GTTTGCTTAC 
AGCTTCATCG TTCTCTTAAA AAAGATGGCT AAGAACTCCC GCACCAGCCT AGCCAGTGCT 
TCCAATGAAA ATTACACCTT CTGCTGGCGG GTGTTTTGTG CCTGGGATTA TCTCATTGGG 
AACCCAGAGG CTGCAGAAAG CAAAACAGCT GCCATCTTGA ACAGCATTAG GGAGGCCATA 
CTGGAAGAGC AAGAAAAGAA AAAAAACAAA AACATGGCGG TGACTGTCTG TCTGAGAATC 
ATTGCCAACA TCCTGGTGCT CCTCTCACTG GCTGGAAGCA TCTATCTCAT CTACTTTGTG 
GTGGACCGGT CCCAGAAGCT GGAGCAGTCG AAGAAGGAGC TGACACTTTG GGAAAAGAAT 
GAGGTAAGCG TGGTGGTCTC CCTCGTCACC ATGCTAGCAC CGTCTGCCTT TGACCTCATC 
GCTGCCTTGG AGATGTATCA TCCACGGACG ACACTGCGCT TCCAGTTAGC AAGGGTCCTG 
GTACTGTATT TAGGAAATCT CTACAGCTTG ATCATTGCTC TTCTGGACAA AGTGAACAGC 
ATGAACATTG AGGAAGCTGC TACTAAAAAC ATAACAAGCC ACTGGGCAGA TGCTCCCACC 
TTCTCTGCCA CCAGGACAGT GCCTGAAGAA GGGCAGTGGC CCACACCTGG ATCAGGAGCG 
GAGCTCAGGA GAAACACTAG CACATGGGTC GTAGAAGAGA CAAGCTTTCT GACTTCTATC 
ACGCCACATA CAAAGGCCAA CAAGACAGTG CCCTACATGC AGGGCCCACA AGGCCAGTGC 
TGGGAGACAT ATGTCGGCCA GGAGATGCTG AAGCTGTCTG TCATCGACAT GCTCTTCACT 
GTGGCCAGCA TCCTCCTCAT AGACTTCTTC CGAGGACTTT TTGTTCGCTA CCTAAGTGAC 
TATTGGTGTT GGGACCTTGA AAGCAAGTTT CCTGAATATG GAGAATTCAA GATTGCCGAG 
AATGTGCTGC ACCTTGTCTA CAATCAAGGC ATGATTTGGA TGGGGGCCTT CTTCTCTCCG 
TGTCTGCCAG CATTCAATGT TCTTAAGCTC ATTGGGCTGA TGTACCTGAG GAGCTGGGCG 
GTGCTGACCT GCAATGTACC CCACCAGCAA GTGTTTCGAG CTTCCAGATC CAACAACTTC 
TACCTGGCAA TGCTCCTGTT CATGTTGTTC CTTTGCATGC TGCCCACCAT TTTTGCCATT 
GTCCACTACA AGCCGTCTCT GAACTGTGGT CCCTTCAGCG GACAAGAGAA AATTTATGAC 
ATCGTGTCCG AGACGATAGA GAATGACTTC CCGACCTGGT TTCACGCTGT AGTTGGACAT 
ATCAGCAGCC CCGTGGTGAT ACTTCCAGCT GTGCTGCTTC TGTTCATGCT CATCTACTAC 
CTTCAGAGCA TCGCGAGATC ACTCAAGCTC AGCAGCCAGC AACTCAGGAT GCAGATCCAA 
AATGCGAGAT CTGAAGATAA GAAAAAGGTT GCCCAAATGG TAGAGGCCCT AGCCATCCCT 
AGCGATGCCA GGCAGGCAGG CTCAGCCACT GAAGCAGAGT CGAGTGAAAA TTCAAAGCCA 
AAGACTCTCC AAGCTCGAAT CCAGACTCAT GAGGAAAGCA GCAAGAGGCT TCTAAAGGAC 
AGCGACCTTA TCAGCCAGCT GTCCTCAGTG TATATGGCAA CATCCCCAAA CAATGGCCAT 
ATGCTTAACT TCGACTCTCT GAGTAGCAAG AGCCTCAGGA TGGAGGCAAT CACAAGGTCC 
CTGCCACAGA GTCCTGGACA AGGGTCCAGG GACCCCTGCT CTCCTCTTCT AGATGGATCT 
AGGTCAAGGC CAGAGCAGGA CACCAACAGG CATCCACATA GGCCATGTTC CAGCACAAGC 
AACCTCCACA AGAACAGATC CTGCTCATCT GTGACACAGA CACAGCCCCT CAAAGATGTC 
CGCTCAGAGC CTCTTTCCCG GAAAGACTTT CAGCCAATCA GTCCTCCTTT CTGTGGATCT 
GGGGTCTCTA CCTTGATGAC ACATGACCAC AGTCCCCGTG CTCCCAGGTA CTATGTGGTT 
AATGAACGTG ACTCTCACAA AAAGACCCAC CGAGCTTTCT GGCCAGAAAG GCATTTCAAG 
ATAGATGCTT TAGGTGACAT TGTAGAGCTG TACCCCAGGA ATGTGCAGCA GTACATGTCC 
TGGGTTCCCA ACCAGCCTTG CTCTCCACAG CTGAGTGAAG AAGAGGAGGA GATGCTCAGG 
AGAGATTTGG TCCAGTGGTC AATCCCTGCT AGCTCCCTGA CAGACCTGCC TCGGTCTTCT 
TGTTTTTACA CTGGTGACAG ATCAGAAAAT AACACCAGAG ACCCTAAATA CCAGCGAAGG 
GTGTACTACA GATCTGGGGA TAATAGTTTT GAAGACCAGC TTGAGAGGCC CACATTTGTA 
CACAGAAAGC CACGATCCAG GAACGGCCAA TACCCACAGC ATGCCCTGAA GGCCAGAGTC 
AAGGCCAAGT TCGAGCCGTC CTTCACAGAA TCTGATTCTG TGTCAGCAGC ATCCAGCAGT 
GACCATCAAA ACAGCAACAA TGACCAATAC CTGCATGTCA TGTCCAGCCA GGGCAGATTC 
CCAAGGTCTG CCAGCCAGCT GGGCAGGAGG AAGGCCAAGT CCAGGCAGGT ACTACCCACA 
GATCTAAATG ATCTAATTTG TTCAAATGTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011240036.1

Translation

MKTSKASQRYRSIRRNASQCYLYQDSLLLGNSDDSFNADETGDSSDPEQIFQNIQFQKDLMANIRCRPWTMGQKLRALRRAKEIVLKFEGRLTRTRGYQAAGAELWRKFARLACNFVVIFIPWEMRIKKIESHFGSGVASYFIFLRWLFGINIVLTVMTGAFVVLPELIAGQPFGSTASKTIPREQITSAQDLDTVWSLGGYLQYSVLFYGYYGRERRIGRAGYRLPLAYFLVGMAVFAYSFIVLLKKMAKNSRTSLASASNENYTFCWRVFCAWDYLIGNPEAAESKTAAILNSIREAILEEQEKKKNKNMAVTVCLRIIANILVLLSLAGSIYLIYFVVDRSQKLEQSKKELTLWEKNEVSVVVSLVTMLAPSAFDLIAALEMYHPRTTLRFQLARVLVLYLGNLYSLIIALLDKVNSMNIEEAATKNITSHWADAPTFSATRTVPEEGQWPTPGSGAELRRNTSTWVVEETSFLTSITPHTKANKTVPYMQGPQGQCWETYVGQEMLKLSVIDMLFTVASILLIDFFRGLFVRYLSDYWCWDLESKFPEYGEFKIAENVLHLVYNQGMIWMGAFFSPCLPAFNVLKLIGLMYLRSWAVLTCNVPHQQVFRASRSNNFYLAMLLFMLFLCMLPTIFAIVHYKPSLNCGPFSGQEKIYDIVSETIENDFPTWFHAVVGHISSPVVILPAVLLLFMLIYYLQSIARSLKLSSQQLRMQIQNARSEDKKKVAQMVEALAIPSDARQAGSATEAESSENSKPKTLQARIQTHEESSKRLLKDSDLISQLSSVYMATSPNNGHMLNFDSLSSKSLRMEAITRSLPQSPGQGSRDPCSPLLDGSRSRPEQDTNRHPHRPCSSTSNLHKNRSCSSVTQTQPLKDVRSEPLSRKDFQPISPPFCGSGVSTLMTHDHSPRAPRYYVVNERDSHKKTHRAFWPERHFKIDALGDIVELYPRNVQQYMSWVPNQPCSPQLSEEEEEMLRRDLVQWSIPASSLTDLPRSSCFYTGDRSENNTRDPKYQRRVYYRSGDNSFEDQLERPTFVHRKPRSRNGQYPQHALKARVKAKFEPSFTESDSVSAASSSDHQNSNNDQYLHVMSSQGRFPRSASQLGRRKAKSRQVLPTDLNDLICSNV

Target ORF information:

RefSeq Version	XM_011241734.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus transmembrane channel-like gene family 3 (Tmc3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241734.1

ORF Insert Sequence:

ATGAAAACCT CAAAGGCATC CCAGCGCTAC AGAAGCATTC GGAGAAACGC CAGCCAGTGT 
TATCTCTACC AGGACTCCCT GCTTCTTGGT AACTCCGATG ACAGCTTTAA TGCTGATGAA 
ACGGGAGACA GCAGTGATCC GGAACAAATC TTCCAGAATA TACAGTTTCA GAAAGATCTC 
ATGGCAAATA TCCGCTGCAG ACCCTGGACC ATGGGGCAGA AACTGCGGGC ACTGAGACGA 
GCCAAGGAGA TTGTGCTGAA GTTTGAAGGG AGGCTGACCA GGACTCGAGG CTACCAGGCA 
GCAGGAGCAG AGCTCTGGCG GAAATTCGCC CGTCTGGCCT GTAACTTTGT GGTCATCTTC 
ATCCCCTGGG AAATGAGGAT AAAGAAAATT GAGAGTCACT TCGGGTCTGG TGTGGCCTCC 
TACTTCATCT TCCTGAGGTG GCTATTTGGG ATTAACATAG TGCTCACAGT GATGACCGGT 
GCCTTTGTTG TCCTTCCCGA GCTGATCGCA GGCCAGCCCT TTGGAAGTAC AGCCAGCAAG 
ACCATTCCCC GGGAGCAGAT CACATCTGCA CAGGATCTGG ACACTGTCTG GTCCCTGGGG 
GGCTACCTTC AATACTCCGT CCTGTTCTAC GGGTACTACG GAAGAGAGAG GCGGATCGGG 
AGAGCCGGCT ACCGCCTGCC CTTGGCGTAT TTCCTGGTGG GGATGGCAGT GTTTGCTTAC 
AGCTTCATCG TTCTCTTAAA AAAGATGGCT AAGAACTCCC GCACCAGCCT AGCCAGTGCT 
TCCAATGAAA ATTACACCTT CTGCTGGCGG GTGTTTTGTG CCTGGGATTA TCTCATTGGG 
AACCCAGAGG CTGCAGAAAG CAAAACAGCT GCCATCTTGA ACAGCATTAG GGAGGCCATA 
CTGGAAGAGC AAGAAAAGAA AAAAAACAAA AACATGGCGG TGACTGTCTG TCTGAGAATC 
ATTGCCAACA TCCTGGTGCT CCTCTCACTG GCTGGAAGCA TCTATCTCAT CTACTTTGTG 
GTGGACCGGT CCCAGAAGCT GGAGCAGTCG AAGAAGGAGC TGACACTTTG GGAAAAGAAT 
GAGGTAAGCG TGGTGGTCTC CCTCGTCACC ATGCTAGCAC CGTCTGCCTT TGACCTCATC 
GCTGCCTTGG AGATGTATCA TCCACGGACG ACACTGCGCT TCCAGTTAGC AAGGGTCCTG 
GTACTGTATT TAGGAAATCT CTACAGCTTG ATCATTGCTC TTCTGGACAA AGTGAACAGC 
ATGAACATTG AGGAAGCTGC TACTAAAAAC ATAACAAGCC ACTGGGCAGA TGCTCCCACC 
TTCTCTGCCA CCAGGACAGT GCCTGAAGAA GGGCAGTGGC CCACACCTGG ATCAGGAGCG 
GAGCTCAGGA GAAACACTAG CACATGGGTC GTAGAAGAGA CAAGCTTTCT GACTTCTATC 
ACGCCACATA CAAAGGCCAA CAAGACAGTG CCCTACATGC AGGGCCCACA AGGCCAGTGC 
TGGGAGACAT ATGTCGGCCA GGAGATGCTG AAGCTGTCTG TCATCGACAT GCTCTTCACT 
GTGGCCAGCA TCCTCCTCAT AGACTTCTTC CGAGGACTTT TTGTTCGCTA CCTAAGTGAC 
TATTGGTGTT GGGACCTTGA AAGCAAGTTT CCTGAATATG GAGAATTCAA GATTGCCGAG 
AATGTGCTGC ACCTTGTCTA CAATCAAGGC ATGATTTGGA TGGGGGCCTT CTTCTCTCCG 
TGTCTGCCAG CATTCAATGT TCTTAAGCTC ATTGGGCTGA TGTACCTGAG GAGCTGGGCG 
GTGCTGACCT GCAATGTACC CCACCAGCAA GTGTTTCGAG CTTCCAGATC CAACAACTTC 
TACCTGGCAA TGCTCCTGTT CATGTTGTTC CTTTGCATGC TGCCCACCAT TTTTGCCATT 
GTCCACTACA AGCCGTCTCT GAACTGTGGT CCCTTCAGCG GACAAGAGAA AATTTATGAC 
ATCGTGTCCG AGACGATAGA GAATGACTTC CCGACCTGGT TTCACGCTGT AGTTGGACAT 
ATCAGCAGCC CCGTGGTGAT ACTTCCAGCT GTGCTGCTTC TGTTCATGCT CATCTACTAC 
CTTCAGAGCA TCGCGAGATC ACTCAAGCTC AGCAGCCAGC AACTCAGGAT GCAGATCCAA 
AATGCGAGAT CTGAAGATAA GAAAAAGGTT GCCCAAATGG TAGAGGCCCT AGCCATCCCT 
AGCGATGCCA GGCAGGCAGG CTCAGCCACT GAAGCAGAGT CGAGTGAAAA TTCAAAGCCA 
AAGACTCTCC AAGCTCGAAT CCAGACTCAT GAGGAAAGCA GCAAGAGGCT TCTAAAGGAC 
AGCGACCTTA TCAGCCAGCT GTCCTCAGTG TATATGGCAA CATCCCCAAA CAATGGCCAT 
ATGCTTAACT TCGACTCTCT GAGTAGCAAG AGCCTCAGGA TGGAGGCAAT CACAAGGTCC 
CTGCCACAGA GTCCTGGACA AGGGTCCAGG GACCCCTGCT CTCCTCTTCT AGATGGATCT 
AGGTCAAGGC CAGAGCAGGA CACCAACAGG CATCCACATA GGCCATGTTC CAGCACAAGC 
AACCTCCACA AGAACAGATC CTGCTCATCT GTGACACAGA CACAGCCCCT CAAAGATGTC 
CGCTCAGAGC CTCTTTCCCG GAAAGACTTT CAGCCAATCA GTCCTCCTTT CTGTGGATCT 
GGGGTCTCTA CCTTGATGAC ACATGACCAC AGTCCCCGTG CTCCCAGGTA CTATGTGGTT 
AATGAACGTG ACTCTCACAA AAAGACCCAC CGAGCTTTCT GGCCAGAAAG GCATTTCAAG 
ATAGATGCTT TAGGTGACAT TGTAGAGCTG TACCCCAGGA ATGTGCAGCA GTACATGTCC 
TGGGTTCCCA ACCAGCCTTG CTCTCCACAG CTGAGTGAAG AAGAGGAGGA GATGCTCAGG 
AGAGATTTGG TCCAGTGGTC AATCCCTGCT AGCTCCCTGA CAGACCTGCC TCGGTCTTCT 
TGTTTTTACA CTGGTGACAG ATCAGAAAAT AACACCAGAG ACCCTAAATA CCAGCGAAGG 
GTGTACTACA GATCTGGGGA TAATAGTTTT GAAGACCAGC TTGAGAGGCC CACATTTGTA 
CACAGAAAGC CACGATCCAG GAACGGCCAA TACCCACAGC ATGCCCTGAA GGCCAGAGTC 
AAGGCCAAGT TCGAGCCGTC CTTCACAGAA TCTGATTCTG TGTCAGCAGC ATCCAGCAGT 
GACCATCAAA ACAGCAACAA TGACCAATAC CTGCATGTCA TGTCCAGCCA GGGCAGATTC 
CCAAGGTCTG CCAGCCAGCT GGGCAGGAGG AAGGCCAAGT CCAGGCAGGT ACTACCCACA 
GATCTAAATG ATCTAATTTG TTCAAATGTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMC3 ( XM_006507591.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006507654.1 Mus musculus transmembrane channel-like gene family 3 (Tmc3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu39457
Clone ID Related Accession (Same CDS sequence)	XM_006507591.3 , XM_006507591.2
Accession Version	XM_006507591.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transmembrane channel-like protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006507591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACCT CAAAGGCATC CCAGCGCTAC AGAAGCATTC GGAGAAACGC CAGCCAGTGT 
TATCTCTACC AGGACTCCCT GCTTCTTGGT AACTCCGATG ACAGCTTTAA TGCTGATGAA 
ACGGGAGACA GCAGTGATCC GGAACAAATC TTCCAGAATA TACAGTTTCA GAAAGATCTC 
ATGGCAAATA TCCGCTGCAG ACCCTGGACC ATGGGGCAGA AACTGCGGGC ACTGAGACGA 
GCCAAGGAGA TTGTGCTGAA GTTTGAAGGG AGGCTGACCA GGACTCGAGG CTACCAGGCA 
GCAGGAGCAG AGCTCTGGCG GAAATTCGCC CGTCTGGCCT GTAACTTTGT GGTCATCTTC 
ATCCCCTGGG AAATGAGGAT AAAGAAAATT GAGAGTCACT TCGGGTCTGG TGTGGCCTCC 
TACTTCATCT TCCTGAGGTG GCTATTTGGG ATTAACATAG TGCTCACAGT GATGACCGGT 
GCCTTTGTTG TCCTTCCCGA GCTGATCGCA GGCCAGCCCT TTGGAAGTAC AGCCAGCAAG 
ACCATTCCCC GGGAGCAGAT CACATCTGCA CAGGATCTGG ACACTGTCTG GTCCCTGGGG 
GGCTACCTTC AATACTCCGT CCTGTTCTAC GGGTACTACG GAAGAGAGAG GCGGATCGGG 
AGAGCCGGCT ACCGCCTGCC CTTGGCGTAT TTCCTGGTGG GGATGGCAGT GTTTGCTTAC 
AGCTTCATCG TTCTCTTAAA AAAGATGGCT AAGAACTCCC GCACCAGCCT AGCCAGTGCT 
TCCAATGAAA ATTACACCTT CTGCTGGCGG GTGTTTTGTG CCTGGGATTA TCTCATTGGG 
AACCCAGAGG CTGCAGAAAG CAAAACAGCT GCCATCTTGA ACAGCATTAG GGAGGCCATA 
CTGGAAGAGC AAGAAAAGAA AAAAAACAAA AACATGGCGG TGACTGTCTG TCTGAGAATC 
ATTGCCAACA TCCTGGTGCT CCTCTCACTG GCTGGAAGCA TCTATCTCAT CTACTTTGTG 
GTGGACCGGT CCCAGAAGCT GGAGCAGTCG AAGAAGGAGC TGACACTTTG GGAAAAGAAT 
GAGGTAAGCG TGGTGGTCTC CCTCGTCACC ATGCTAGCAC CGTCTGCCTT TGACCTCATC 
GCTGCCTTGG AGATGTATCA TCCACGGACG ACACTGCGCT TCCAGTTAGC AAGGGTCCTG 
GTACTGTATT TAGGAAATCT CTACAGCTTG ATCATTGCTC TTCTGGACAA AGTGAACAGC 
ATGAACATTG AGGAAGCTGC TACTAAAAAC ATAACAAGCC ACTGGGCAGA TGCTCCCACC 
TTCTCTGCCA CCAGGACAGT GCCTGAAGAA GGGCAGTGGC CCACACCTGG ATCAGGAGCG 
GAGCTCAGGA GAAACACTAG CACATGGGTC GTAGAAGAGA CAAGCTTTCT GACTTCTATC 
ACGCCACATA CAAAGGCCAA CAAGACAGTG CCCTACATGC AGGGCCCACA AGGCCAGTGC 
TGGGAGACAT ATGTCGGCCA GGAGATGCTG AAGCTGTCTG TCATCGACAT GCTCTTCACT 
GTGGCCAGCA TCCTCCTCAT AGACTTCTTC CGAGGACTTT TTGTTCGCTA CCTAAGTGAC 
TATTGGTGTT GGGACCTTGA AAGCAAGTTT CCTGAATATG GAGAATTCAA GATTGCCGAG 
AATGTGCTGC ACCTTGTCTA CAATCAAGGC ATGATTTGGA TGGGGGCCTT CTTCTCTCCG 
TGTCTGCCAG CATTCAATGT TCTTAAGCTC ATTGGGCTGA TGTACCTGAG GAGCTGGGCG 
GTGCTGACCT GCAATGTACC CCACCAGCAA GTGTTTCGAG CTTCCAGATC CAACAACTTC 
TACCTGGCAA TGCTCCTGTT CATGTTGTTC CTTTGCATGC TGCCCACCAT TTTTGCCATT 
GTCCACTACA AGCCGTCTCT GAACTGTGGT CCCTTCAGCG GACAAGAGAA AATTTATGAC 
ATCGTGTCCG AGACGATAGA GAATGACTTC CCGACCTGGT TTCACGCTGT AGTTGGACAT 
ATCAGCAGCC CCGTGGTGAT ACTTCCAGCT GTGCTGCTTC TGTTCATGCT CATCTACTAC 
CTTCAGAGCA TCGCGAGATC ACTCAAGCTC AGCAGCCAGC AACTCAGGAT GCAGATCCAA 
AATGCGAGAT CTGAAGATAA GAAAAAGGTT GCCCAAATGG TAGAGGCTCG AATCCAGACT 
CATGAGGAAA GCAGCAAGAG GCTTCTAAAG GACAGCGACC TTATCAGCCA GCTGTCCTCA 
GTGTATATGG CAACATCCCC AAACAATGGC CATATGCTTA ACTTCGACTC TCTGAGTAGC 
AAGAGCCTCA GGATGGAGGC AATCACAAGG TCCCTGCCAC AGAGTCCTGG ACAAGGGTCC 
AGGGACCCCT GCTCTCCTCT TCTAGATGGA TCTAGGTCAA GGCCAGAGCA GGACACCAAC 
AGGCATCCAC ATAGGCCATG TTCCAGCACA AGCAACCTCC ACAAGAACAG ATCCTGCTCA 
TCTGTGACAC AGACACAGCC CCTCAAAGAT GTCCGCTCAG AGCCTCTTTC CCGGAAAGAC 
TTTCAGCCAA TCAGTCCTCC TTTCTGTGGA TCTGGGGTCT CTACCTTGAT GACACATGAC 
CACAGTCCCC GTGCTCCCAG GTACTATGTG GTTAATGAAC GTGACTCTCA CAAAAAGACC 
CACCGAGCTT TCTGGCCAGA AAGGCATTTC AAGATAGATG CTTTAGGTGA CATTGTAGAG 
CTGTACCCCA GGAATGTGCA GCAGTACATG TCCTGGGTTC CCAACCAGCC TTGCTCTCCA 
CAGCTGAGTG AAGAAGAGGA GGAGATGCTC AGGAGAGATT TGGTCCAGTG GTCAATCCCT 
GCTAGCTCCC TGACAGACCT GCCTCGGTCT TCTTGTTTTT ACACTGGTGA CAGATCAGAA 
AATAACACCA GAGACCCTAA ATACCAGCGA AGGGTGTACT ACAGATCTGG GGATAATAGT 
TTTGAAGACC AGCTTGAGAG GCCCACATTT GTACACAGAA AGCCACGATC CAGGAACGGC 
CAATACCCAC AGCATGCCCT GAAGGCCAGA GTCAAGGCCA AGTTCGAGCC GTCCTTCACA 
GAATCTGATT CTGTGTCAGC AGCATCCAGC AGTGACCATC AAAACAGCAA CAATGACCAA 
TACCTGCATG TCATGTCCAG CCAGGGCAGA TTCCCAAGGT CTGCCAGCCA GCTGGGCAGG 
AGGAAGGCCA AGTCCAGGCA GGTACTACCC ACAGATCTAA ATGATCTAAT TTGTTCAAAT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507654.1
CDS	792..4097
Translation	MKTSKASQRYRSIRRNASQCYLYQDSLLLGNSDDSFNADETGDSSDPEQIFQNIQFQKDLMANIRCRPWTMGQKLRALRRAKEIVLKFEGRLTRTRGYQAAGAELWRKFARLACNFVVIFIPWEMRIKKIESHFGSGVASYFIFLRWLFGINIVLTVMTGAFVVLPELIAGQPFGSTASKTIPREQITSAQDLDTVWSLGGYLQYSVLFYGYYGRERRIGRAGYRLPLAYFLVGMAVFAYSFIVLLKKMAKNSRTSLASASNENYTFCWRVFCAWDYLIGNPEAAESKTAAILNSIREAILEEQEKKKNKNMAVTVCLRIIANILVLLSLAGSIYLIYFVVDRSQKLEQSKKELTLWEKNEVSVVVSLVTMLAPSAFDLIAALEMYHPRTTLRFQLARVLVLYLGNLYSLIIALLDKVNSMNIEEAATKNITSHWADAPTFSATRTVPEEGQWPTPGSGAELRRNTSTWVVEETSFLTSITPHTKANKTVPYMQGPQGQCWETYVGQEMLKLSVIDMLFTVASILLIDFFRGLFVRYLSDYWCWDLESKFPEYGEFKIAENVLHLVYNQGMIWMGAFFSPCLPAFNVLKLIGLMYLRSWAVLTCNVPHQQVFRASRSNNFYLAMLLFMLFLCMLPTIFAIVHYKPSLNCGPFSGQEKIYDIVSETIENDFPTWFHAVVGHISSPVVILPAVLLLFMLIYYLQSIARSLKLSSQQLRMQIQNARSEDKKKVAQMVEARIQTHEESSKRLLKDSDLISQLSSVYMATSPNNGHMLNFDSLSSKSLRMEAITRSLPQSPGQGSRDPCSPLLDGSRSRPEQDTNRHPHRPCSSTSNLHKNRSCSSVTQTQPLKDVRSEPLSRKDFQPISPPFCGSGVSTLMTHDHSPRAPRYYVVNERDSHKKTHRAFWPERHFKIDALGDIVELYPRNVQQYMSWVPNQPCSPQLSEEEEEMLRRDLVQWSIPASSLTDLPRSSCFYTGDRSENNTRDPKYQRRVYYRSGDNSFEDQLERPTFVHRKPRSRNGQYPQHALKARVKAKFEPSFTESDSVSAASSSDHQNSNNDQYLHVMSSQGRFPRSASQLGRRKAKSRQVLPTDLNDLICSNV

Target ORF information:

RefSeq Version	XM_006507591.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane channel-like gene family 3 (Tmc3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507591.2

ORF Insert Sequence:

ATGAAAACCT CAAAGGCATC CCAGCGCTAC AGAAGCATTC GGAGAAACGC CAGCCAGTGT 
TATCTCTACC AGGACTCCCT GCTTCTTGGT AACTCCGATG ACAGCTTTAA TGCTGATGAA 
ACGGGAGACA GCAGTGATCC GGAACAAATC TTCCAGAATA TACAGTTTCA GAAAGATCTC 
ATGGCAAATA TCCGCTGCAG ACCCTGGACC ATGGGGCAGA AACTGCGGGC ACTGAGACGA 
GCCAAGGAGA TTGTGCTGAA GTTTGAAGGG AGGCTGACCA GGACTCGAGG CTACCAGGCA 
GCAGGAGCAG AGCTCTGGCG GAAATTCGCC CGTCTGGCCT GTAACTTTGT GGTCATCTTC 
ATCCCCTGGG AAATGAGGAT AAAGAAAATT GAGAGTCACT TCGGGTCTGG TGTGGCCTCC 
TACTTCATCT TCCTGAGGTG GCTATTTGGG ATTAACATAG TGCTCACAGT GATGACCGGT 
GCCTTTGTTG TCCTTCCCGA GCTGATCGCA GGCCAGCCCT TTGGAAGTAC AGCCAGCAAG 
ACCATTCCCC GGGAGCAGAT CACATCTGCA CAGGATCTGG ACACTGTCTG GTCCCTGGGG 
GGCTACCTTC AATACTCCGT CCTGTTCTAC GGGTACTACG GAAGAGAGAG GCGGATCGGG 
AGAGCCGGCT ACCGCCTGCC CTTGGCGTAT TTCCTGGTGG GGATGGCAGT GTTTGCTTAC 
AGCTTCATCG TTCTCTTAAA AAAGATGGCT AAGAACTCCC GCACCAGCCT AGCCAGTGCT 
TCCAATGAAA ATTACACCTT CTGCTGGCGG GTGTTTTGTG CCTGGGATTA TCTCATTGGG 
AACCCAGAGG CTGCAGAAAG CAAAACAGCT GCCATCTTGA ACAGCATTAG GGAGGCCATA 
CTGGAAGAGC AAGAAAAGAA AAAAAACAAA AACATGGCGG TGACTGTCTG TCTGAGAATC 
ATTGCCAACA TCCTGGTGCT CCTCTCACTG GCTGGAAGCA TCTATCTCAT CTACTTTGTG 
GTGGACCGGT CCCAGAAGCT GGAGCAGTCG AAGAAGGAGC TGACACTTTG GGAAAAGAAT 
GAGGTAAGCG TGGTGGTCTC CCTCGTCACC ATGCTAGCAC CGTCTGCCTT TGACCTCATC 
GCTGCCTTGG AGATGTATCA TCCACGGACG ACACTGCGCT TCCAGTTAGC AAGGGTCCTG 
GTACTGTATT TAGGAAATCT CTACAGCTTG ATCATTGCTC TTCTGGACAA AGTGAACAGC 
ATGAACATTG AGGAAGCTGC TACTAAAAAC ATAACAAGCC ACTGGGCAGA TGCTCCCACC 
TTCTCTGCCA CCAGGACAGT GCCTGAAGAA GGGCAGTGGC CCACACCTGG ATCAGGAGCG 
GAGCTCAGGA GAAACACTAG CACATGGGTC GTAGAAGAGA CAAGCTTTCT GACTTCTATC 
ACGCCACATA CAAAGGCCAA CAAGACAGTG CCCTACATGC AGGGCCCACA AGGCCAGTGC 
TGGGAGACAT ATGTCGGCCA GGAGATGCTG AAGCTGTCTG TCATCGACAT GCTCTTCACT 
GTGGCCAGCA TCCTCCTCAT AGACTTCTTC CGAGGACTTT TTGTTCGCTA CCTAAGTGAC 
TATTGGTGTT GGGACCTTGA AAGCAAGTTT CCTGAATATG GAGAATTCAA GATTGCCGAG 
AATGTGCTGC ACCTTGTCTA CAATCAAGGC ATGATTTGGA TGGGGGCCTT CTTCTCTCCG 
TGTCTGCCAG CATTCAATGT TCTTAAGCTC ATTGGGCTGA TGTACCTGAG GAGCTGGGCG 
GTGCTGACCT GCAATGTACC CCACCAGCAA GTGTTTCGAG CTTCCAGATC CAACAACTTC 
TACCTGGCAA TGCTCCTGTT CATGTTGTTC CTTTGCATGC TGCCCACCAT TTTTGCCATT 
GTCCACTACA AGCCGTCTCT GAACTGTGGT CCCTTCAGCG GACAAGAGAA AATTTATGAC 
ATCGTGTCCG AGACGATAGA GAATGACTTC CCGACCTGGT TTCACGCTGT AGTTGGACAT 
ATCAGCAGCC CCGTGGTGAT ACTTCCAGCT GTGCTGCTTC TGTTCATGCT CATCTACTAC 
CTTCAGAGCA TCGCGAGATC ACTCAAGCTC AGCAGCCAGC AACTCAGGAT GCAGATCCAA 
AATGCGAGAT CTGAAGATAA GAAAAAGGTT GCCCAAATGG TAGAGGCTCG AATCCAGACT 
CATGAGGAAA GCAGCAAGAG GCTTCTAAAG GACAGCGACC TTATCAGCCA GCTGTCCTCA 
GTGTATATGG CAACATCCCC AAACAATGGC CATATGCTTA ACTTCGACTC TCTGAGTAGC 
AAGAGCCTCA GGATGGAGGC AATCACAAGG TCCCTGCCAC AGAGTCCTGG ACAAGGGTCC 
AGGGACCCCT GCTCTCCTCT TCTAGATGGA TCTAGGTCAA GGCCAGAGCA GGACACCAAC 
AGGCATCCAC ATAGGCCATG TTCCAGCACA AGCAACCTCC ACAAGAACAG ATCCTGCTCA 
TCTGTGACAC AGACACAGCC CCTCAAAGAT GTCCGCTCAG AGCCTCTTTC CCGGAAAGAC 
TTTCAGCCAA TCAGTCCTCC TTTCTGTGGA TCTGGGGTCT CTACCTTGAT GACACATGAC 
CACAGTCCCC GTGCTCCCAG GTACTATGTG GTTAATGAAC GTGACTCTCA CAAAAAGACC 
CACCGAGCTT TCTGGCCAGA AAGGCATTTC AAGATAGATG CTTTAGGTGA CATTGTAGAG 
CTGTACCCCA GGAATGTGCA GCAGTACATG TCCTGGGTTC CCAACCAGCC TTGCTCTCCA 
CAGCTGAGTG AAGAAGAGGA GGAGATGCTC AGGAGAGATT TGGTCCAGTG GTCAATCCCT 
GCTAGCTCCC TGACAGACCT GCCTCGGTCT TCTTGTTTTT ACACTGGTGA CAGATCAGAA 
AATAACACCA GAGACCCTAA ATACCAGCGA AGGGTGTACT ACAGATCTGG GGATAATAGT 
TTTGAAGACC AGCTTGAGAG GCCCACATTT GTACACAGAA AGCCACGATC CAGGAACGGC 
CAATACCCAC AGCATGCCCT GAAGGCCAGA GTCAAGGCCA AGTTCGAGCC GTCCTTCACA 
GAATCTGATT CTGTGTCAGC AGCATCCAGC AGTGACCATC AAAACAGCAA CAATGACCAA 
TACCTGCATG TCATGTCCAG CCAGGGCAGA TTCCCAAGGT CTGCCAGCCA GCTGGGCAGG 
AGGAAGGCCA AGTCCAGGCA GGTACTACCC ACAGATCTAA ATGATCTAAT TTGTTCAAAT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Conductance and block of hair-cell mechanotransducer channels in transmembrane channel-like protein mutants.
The Journal of general physiology144(1)55-69(2014 Jul)
Beurg M,Kim KX,Fettiplace R

Characterization of the transmembrane channel-like (TMC) gene family: functional clues from hearing loss and epidermodysplasia verruciformis.
Genomics82(3)300-8(2003 Sep)
Kurima K,Yang Y,Sorber K,Griffith AJ

TMC and EVER genes belong to a larger novel family, the TMC gene family encoding transmembrane proteins.
BMC genomics4(1)24(2003 Jun)
Keresztes G,Mutai H,Heller S