Mepce cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mepce
Entrez Gene ID	231803
Full Name	methylphosphate capping enzyme
Synonyms	Bcdin3,D5Wsu46e
General protein information	Preferred Names methylphosphate capping enzyme Names 7SK snRNA methylphosphate capping enzyme bin3, bicoid-interacting 3, homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 G2\|5 76.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_144913.3	NP_659162.3	7SK snRNA methylphosphate capping enzyme
XM_017320873.1	XP_017176362.1	7SK snRNA methylphosphate capping enzyme isoform X1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT5G51130	NP_568752.1
Bos taurus (cattle)	MEPCE	NP_001073752.2
Pan troglodytes (chimpanzee)	MEPCE	XP_527836.2
Oryza sativa (rice)	Os08g0540500	NP_001062384.1
Homo sapiens (human)	MEPCE	NP_062552.2
	MEPCE	XP_001104039.1
Canis lupus familiaris (dog)	MEPCE	XP_546957.2
Mus musculus (house mouse)	Mepce	NP_659162.3

Gene Ontology
Bibliography

Related articles in PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells.
Okamura D, Maeda I, Taniguchi H, Tokitake Y, Ikeda M, Ozato K, Mise N, Abe K, Noce T, Izpisua Belmonte JC, Matsui Y
Genes & development26(22)2477-82(2012 Nov)

Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome.
Ko MS, Threat TA, Wang X, Horton JH, Cui Y, Wang X, Pryor E, Paris J, Wells-Smith J, Kitchen JR, Rowe LB, Eppig J, Satoh T, Brant L, Fujiwara H, Yotsumoto S, Nakashima H
Human molecular genetics7(12)1967-78(1998 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mepce gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mepce cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20778	NM_144913.3 Latest version!	Mus musculus methylphosphate capping enzyme (Mepce), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu81373	XM_017320873.1 Latest version!	Mus musculus methylphosphate capping enzyme (Mepce), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mepce ( NM_144913.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659162.3 Mus musculus methylphosphate capping enzyme (Mepce), mRNA.

Price & Availability↑

CloneID	OMu20778
Clone ID Related Accession (Same CDS sequence)	NM_144913.3
Accession Version	NM_144913.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Mus musculus(house mouse)
Product	7SK snRNA methylphosphate capping enzyme
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125063.4, AK139731.1 and BF323293.1. On Dec 20, 2006 this sequence version replaced NM_144913.2. ##Evidence-Data-START## Transcript exon combination :: BC017157.1, AK154415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGATCGAGA TGGCGGCGGA GAAGGAGCCC TTTCTGGTGC CGGCCCCGCC GCCGCCGCTC 
AAAGATGAGT CGGGCGGAGG GGGCGGCCCC GAGGTGCAAT CGCACCAAGA GGCTGCCTCC 
GGGGAGCTCC GTGATGGGAC AGAACACGGG CCGGGCCCTC GCGCGCACTC GGCCGGGGCC 
GCAGCCAGCG GAGGGGGCGG CCCCCAGGCA CAGGCGCACG GGGAGCCCCA CGGGCGAGCC 
GCCGCTCCTG CGGACGTGGG GGAGGAGCGC CGGGGAGGGG GCGGGACGGA CCTGGGTCCC 
CCAGCCCCTC CTCGACCTCG CAATGGCTAC CAGCCCCACC GACCCCCTGG GGGTGGTGGG 
GGCAAAAGAA GAAATAGCTG TAACGTGGGG GGAGGGAGCG GTGGAAGCTT CAAACATCCG 
GCCTTCAAGA GGCGCCGGCG AGTGAATTCA GACTGCGACT CTGTGTTACC ATCCAATTTC 
CTTCTGGGGG GTAACATATT TGATCCACTG AACCTGAATA GCCTCCTGGA TGAGGAAGTG 
AGTCGCGCAC TCAATGCGGA GACCCCAAAG TCATCCCCAC TTCCGGCCAA GGGGCGAGAC 
CCTGTGGAGA TCCTCATCCC CAAAGATATT ACTGACCCGC TCAGTCTCAA CACTTGCACT 
GACGAGGCCC ATGTAGTGCT TGCCTCACCA CTCAAGATTG GTCGCAAGCG CCATAGACAC 
CGGGGACCGC ACCACCAGCA GCAGCAGCAG GCGTCTGGAG GGAATGATAG CAACGCCGCC 
GTGCTGCCCA CTGACCCTCT CACGCCCTCA CTCCATGGGG AGGGTGCTAC GCAGCAGCAG 
CAAAACAGAG GCCAGAACCG TGATGCCCCT CAACCCTATG AACTCAACAC AGCCATCAAC 
TGCAGGGATG AAGTTGTGTC TCCCCTTCCA TCTGCCCTGC AGGGTTCCTC AGGCTCCCTT 
TCTGCCCCTC CAGCTGCCTC AGTTACCTCT GCACCCTCTA CTTCTTCCTC CTCCCGACAT 
CGCAAACGTC GCAGGACTTC CAGCAAGTCA GAGGCAGGGG CTAGGGGTGG AAGCCAGGGT 
TCCAAGGAAA AGGGCAGAGG GAGTGGAGGA GGCCGTCACC ACCACCACCC ACTACCTGCA 
ACTGGCTTCA AAAAGCAACA GCTAAAGTTC CAATATGGGA ATTACTGCAA GTACTATGGC 
TATCGCAATC CTTCCTGTGA GGACGTGCGC CTTAGGGTGT TGAAGCCCGA GTGGTTTCAA 
GGCCGGGACG TCCTAGATCT GGGCTGCAAC GTTGGCCATC TGACCCTAAG TATTGCCTGC 
AAGTGGGGCC CAGCCCGCAT GGTGGGGCTG GACATTGATC CACGGCTTAT CCACTCTGCT 
CGCCAAAATA TCCGACACTA CCTGTCGGAG GAGCTTCGTC TCCAAGCTCA GACTTCTGAG 
GGGGACCCAG GGACAGAGGG GGAGGAAGGG ACTATCACTG TCCGAAAAAG GAGCTGCTTC 
CCAGCCTCAC TGACAGCCAG CCGGGGTCCC ATCGCTGCAC CCCAAGTGCC CTTGGATGGA 
GCGGACACAT CTGTCTTCCC CAACAATGTC GTCTTCGTCA CGGGTAACTA TGTCCTGGAC 
CGAGATGAGC TGGTGGATGC CCAAAGACCG GAGTATGATG TGGTGCTGTG CTTCAGCCTC 
ACCAAGTGGG TGCACCTGAA CTGGGGTGAT GAGGGGCTGA AGAGAATGTT CCGCCGCATC 
TACCGGCACC TGCGCCCTGG GGGTATTCTG GTCCTGGAGC CCCAACCATG GTCCTCCTAC 
TGCAAGAGAA AGTCTCTCAC GGAAACAATC TATAAGAACT ACTTTCGAAT CCAGTTGAAG 
CCAGAACAGT TCAGTTCCTA CCTGACATCC CCAGAGGTGG GTTTCTCCAG CTATGAGCTT 
GTGGCCACAC CGAATAACAC CTCCAGAGGC TTCCAGCGTC CTGTGTACTT GTTCCACAAG 
GCTCGATCCC CCAGCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659162.3
CDS	640..2640
Translation	MIEMAAEKEPFLVPAPPPPLKDESGGGGGPEVQSHQEAASGELRDGTEHGPGPRAHSAGAAASGGGGPQAQAHGEPHGRAAAPADVGEERRGGGGTDLGPPAPPRPRNGYQPHRPPGGGGGKRRNSCNVGGGSGGSFKHPAFKRRRRVNSDCDSVLPSNFLLGGNIFDPLNLNSLLDEEVSRALNAETPKSSPLPAKGRDPVEILIPKDITDPLSLNTCTDEAHVVLASPLKIGRKRHRHRGPHHQQQQQASGGNDSNAAVLPTDPLTPSLHGEGATQQQQNRGQNRDAPQPYELNTAINCRDEVVSPLPSALQGSSGSLSAPPAASVTSAPSTSSSSRHRKRRRTSSKSEAGARGGSQGSKEKGRGSGGGRHHHHPLPATGFKKQQLKFQYGNYCKYYGYRNPSCEDVRLRVLKPEWFQGRDVLDLGCNVGHLTLSIACKWGPARMVGLDIDPRLIHSARQNIRHYLSEELRLQAQTSEGDPGTEGEEGTITVRKRSCFPASLTASRGPIAAPQVPLDGADTSVFPNNVVFVTGNYVLDRDELVDAQRPEYDVVLCFSLTKWVHLNWGDEGLKRMFRRIYRHLRPGGILVLEPQPWSSYCKRKSLTETIYKNYFRIQLKPEQFSSYLTSPEVGFSSYELVATPNNTSRGFQRPVYLFHKARSPSH

Target ORF information:

RefSeq Version	NM_144913.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus methylphosphate capping enzyme (Mepce), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144913.3

ORF Insert Sequence:

ATGATCGAGA TGGCGGCGGA GAAGGAGCCC TTTCTGGTGC CGGCCCCGCC GCCGCCGCTC 
AAAGATGAGT CGGGCGGAGG GGGCGGCCCC GAGGTGCAAT CGCACCAAGA GGCTGCCTCC 
GGGGAGCTCC GTGATGGGAC AGAACACGGG CCGGGCCCTC GCGCGCACTC GGCCGGGGCC 
GCAGCCAGCG GAGGGGGCGG CCCCCAGGCA CAGGCGCACG GGGAGCCCCA CGGGCGAGCC 
GCCGCTCCTG CGGACGTGGG GGAGGAGCGC CGGGGAGGGG GCGGGACGGA CCTGGGTCCC 
CCAGCCCCTC CTCGACCTCG CAATGGCTAC CAGCCCCACC GACCCCCTGG GGGTGGTGGG 
GGCAAAAGAA GAAATAGCTG TAACGTGGGG GGAGGGAGCG GTGGAAGCTT CAAACATCCG 
GCCTTCAAGA GGCGCCGGCG AGTGAATTCA GACTGCGACT CTGTGTTACC ATCCAATTTC 
CTTCTGGGGG GTAACATATT TGATCCACTG AACCTGAATA GCCTCCTGGA TGAGGAAGTG 
AGTCGCGCAC TCAATGCGGA GACCCCAAAG TCATCCCCAC TTCCGGCCAA GGGGCGAGAC 
CCTGTGGAGA TCCTCATCCC CAAAGATATT ACTGACCCGC TCAGTCTCAA CACTTGCACT 
GACGAGGCCC ATGTAGTGCT TGCCTCACCA CTCAAGATTG GTCGCAAGCG CCATAGACAC 
CGGGGACCGC ACCACCAGCA GCAGCAGCAG GCGTCTGGAG GGAATGATAG CAACGCCGCC 
GTGCTGCCCA CTGACCCTCT CACGCCCTCA CTCCATGGGG AGGGTGCTAC GCAGCAGCAG 
CAAAACAGAG GCCAGAACCG TGATGCCCCT CAACCCTATG AACTCAACAC AGCCATCAAC 
TGCAGGGATG AAGTTGTGTC TCCCCTTCCA TCTGCCCTGC AGGGTTCCTC AGGCTCCCTT 
TCTGCCCCTC CAGCTGCCTC AGTTACCTCT GCACCCTCTA CTTCTTCCTC CTCCCGACAT 
CGCAAACGTC GCAGGACTTC CAGCAAGTCA GAGGCAGGGG CTAGGGGTGG AAGCCAGGGT 
TCCAAGGAAA AGGGCAGAGG GAGTGGAGGA GGCCGTCACC ACCACCACCC ACTACCTGCA 
ACTGGCTTCA AAAAGCAACA GCTAAAGTTC CAATATGGGA ATTACTGCAA GTACTATGGC 
TATCGCAATC CTTCCTGTGA GGACGTGCGC CTTAGGGTGT TGAAGCCCGA GTGGTTTCAA 
GGCCGGGACG TCCTAGATCT GGGCTGCAAC GTTGGCCATC TGACCCTAAG TATTGCCTGC 
AAGTGGGGCC CAGCCCGCAT GGTGGGGCTG GACATTGATC CACGGCTTAT CCACTCTGCT 
CGCCAAAATA TCCGACACTA CCTGTCGGAG GAGCTTCGTC TCCAAGCTCA GACTTCTGAG 
GGGGACCCAG GGACAGAGGG GGAGGAAGGG ACTATCACTG TCCGAAAAAG GAGCTGCTTC 
CCAGCCTCAC TGACAGCCAG CCGGGGTCCC ATCGCTGCAC CCCAAGTGCC CTTGGATGGA 
GCGGACACAT CTGTCTTCCC CAACAATGTC GTCTTCGTCA CGGGTAACTA TGTCCTGGAC 
CGAGATGAGC TGGTGGATGC CCAAAGACCG GAGTATGATG TGGTGCTGTG CTTCAGCCTC 
ACCAAGTGGG TGCACCTGAA CTGGGGTGAT GAGGGGCTGA AGAGAATGTT CCGCCGCATC 
TACCGGCACC TGCGCCCTGG GGGTATTCTG GTCCTGGAGC CCCAACCATG GTCCTCCTAC 
TGCAAGAGAA AGTCTCTCAC GGAAACAATC TATAAGAACT ACTTTCGAAT CCAGTTGAAG 
CCAGAACAGT TCAGTTCCTA CCTGACATCC CCAGAGGTGG GTTTCTCCAG CTATGAGCTT 
GTGGCCACAC CGAATAACAC CTCCAGAGGC TTCCAGCGTC CTGTGTACTT GTTCCACAAG 
GCTCGATCCC CCAGCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mepce ( XM_017320873.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176362.1 Mus musculus methylphosphate capping enzyme (Mepce), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81373
Clone ID Related Accession (Same CDS sequence)	XM_017320873.1
Accession Version	XM_017320873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	7SK snRNA methylphosphate capping enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGAGA TGGCGGCGGA GAAGGAGCCC TTTCTGGTGC CGGCCCCGCC GCCGCCGCTC 
AAAGATGAGT CGGGCGGAGG GGGCGGCCCC GAGGTGCAAT CGCACCAAGA GGCTGCCTCC 
GGGGAGCTCC GTGATGGGAC AGAACACGGG CCGGGCCCTC GCGCGCACTC GGCCGGGGCC 
GCAGCCAGCG GAGGGGGCGG CCCCCAGGCA CAGGCGCACG GGGAGCCCCA CGGGCGAGCC 
GCCGCTCCTG CGGACGTGGG GGAGGAGCGC CGGGGAGGGG GCGGGACGGA CCTGGGTCCC 
CCAGCCCCTC CTCGACCTCG CAATGGCTAC CAGCCCCACC GACCCCCTGG GGGTGGTGGG 
GGCAAAAGAA GAAATAGCTG TAACGTGGGG GGAGGGAGCG GTGGAAGCTT CAAACATCCG 
GCCTTCAAGA GGCGCCGGCG AGTGAATTCA GACTGCGACT CTGTGTTACC ATCCAATTTC 
CTTCTGGGGG GTAACATATT TGATCCACTG AACCTGAATA GCCTCCTGGA TGAGGAAGTG 
AGTCGCGCAC TCAATGCGGA GACCCCAAAG TCATCCCCAC TTCCGGCCAA GGGGCGAGAC 
CCTGTGGAGA TCCTCATCCC CAAAGATATT ACTGACCCGC TCAGTCTCAA CACTTGCACT 
GACGAGGCCC ATGTAGTGCT TGCCTCACCA CTCAAGATTG GTCGCAAGCG CCATAGACAC 
CGGGGACCGC ACCACCAGCA GCAGCAGCAG GCGTCTGGAG GGAATGATAG CAACGCCGCC 
GTGCTGCCCA CTGACCCTCT CACGCCCTCA CTCCATGGGG AGGGTGCTAC GCAGCAGCAG 
CAAAACAGAG GCCAGAACCG TGATGCCCCT CAACCCTATG AACTCAACAC AGCCATCAAC 
TGCAGGGATG AAGTTGTGTC TCCCCTTCCA TCTGCCCTGC AGGGTTCCTC AGGCTCCCTT 
TCTGCCCCTC CAGCTGCCTC AGTTACCTCT GCACCCTCTA CTTCTTCCTC CTCCCGACAT 
CGCAAACGTC GCAGGACTTC CAGCAAGTCA GAGGCAGGGG CTAGGGGTGG AAGCCAGGGT 
TCCAAGGAAA AGGGCAGAGG GAGTGGAGGA GGCCGTCACC ACCACCACCC ACTACCTGCA 
ACTGGCTTCA AAAAGCAACA GCTAAAGTTC CAATATGGGA ATTACTGCAA GTACTATGGC 
TATCGCAATC CTTCCTGTGA GGACGTGCGC CTTAGGGTGT TGAAGCCCGA GTGGTTTCAA 
GGCCGGGACG TCCTAGATCT GGGCTGCAAC GTTGGCCATC TGACCCTAAG TATTGCCTGC 
AAGTGGGGCC CAGCCCGCAT GGTGGGGCTG GACATTGATC CACGGCTTAT CCACTCTGCT 
CGCCAAAATA TCCGACACTA CCTGTCGGAG GAGCTTCGTC TCCAAGCTCA GACTTCTGAG 
GGGGACCCAG GGACAGAGGG GGAGGAAGGG ACTATCACTG TCCGAAAAAG GAGCTGCTTC 
CCAGCCTCAC TGACAGCCAG CCGGGGTCCC ATCGCTGCAC CCCAAGTGCC CTTGGATGGA 
GCGGACACAT CTGTCTTCCC CAACAATGTC GTCTTCGTCA CGGGTAACTA TGTCCTGGAC 
CGAGATGAGC TGGTGGATGC CCAAAGACCG GAGTATGATG TGGTGCTGTG CTTCAGCCTC 
ACCAAGTGGG TGCACCTGAA CTGGGGTGAT GAGGGGCTGA AGAGAATGTT CCGCCGCATC 
TACCGGCACC TGCGCCCTGG GGGTATTCTG GTCCTGGAGC CCCAACCATG GTCCTCCTAC 
TGCAAGAGAA AGTCTCTCAC GGAAACAATC TATAAGAACT ACTTTCGAAT CCAGTTGAAG 
CCAGAACAGT TCAGTTCCTA CCTGACATCC CCAGAGGTGG GTTTCTCCAG CTATGAGCTT 
GTGGCCACAC CGAATAACAC CTCCAGAGGT AAGAAGGGCT CGTTTTTTTG GGCTGGGAGG 
GGATCGTCCT GGCTGCAAGG GTATAGGCCC TGTGGAGAAC CCCAGGGTCC TACCCACCTG 
CCTCTGTCTT CCCTTCAGGC TTCCAGCGTC CTGTGTACTT GTTCCACAAG GCTCGATCCC 
CCAGCCACTA AGTGGCCTTC TGAGAAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176362.1
CDS	526..2658
Translation	MIEMAAEKEPFLVPAPPPPLKDESGGGGGPEVQSHQEAASGELRDGTEHGPGPRAHSAGAAASGGGGPQAQAHGEPHGRAAAPADVGEERRGGGGTDLGPPAPPRPRNGYQPHRPPGGGGGKRRNSCNVGGGSGGSFKHPAFKRRRRVNSDCDSVLPSNFLLGGNIFDPLNLNSLLDEEVSRALNAETPKSSPLPAKGRDPVEILIPKDITDPLSLNTCTDEAHVVLASPLKIGRKRHRHRGPHHQQQQQASGGNDSNAAVLPTDPLTPSLHGEGATQQQQNRGQNRDAPQPYELNTAINCRDEVVSPLPSALQGSSGSLSAPPAASVTSAPSTSSSSRHRKRRRTSSKSEAGARGGSQGSKEKGRGSGGGRHHHHPLPATGFKKQQLKFQYGNYCKYYGYRNPSCEDVRLRVLKPEWFQGRDVLDLGCNVGHLTLSIACKWGPARMVGLDIDPRLIHSARQNIRHYLSEELRLQAQTSEGDPGTEGEEGTITVRKRSCFPASLTASRGPIAAPQVPLDGADTSVFPNNVVFVTGNYVLDRDELVDAQRPEYDVVLCFSLTKWVHLNWGDEGLKRMFRRIYRHLRPGGILVLEPQPWSSYCKRKSLTETIYKNYFRIQLKPEQFSSYLTSPEVGFSSYELVATPNNTSRGKKGSFFWAGRGSSWLQGYRPCGEPQGPTHLPLSSLQASSVLCTCSTRLDPPATKWPSEKL

Target ORF information:

RefSeq Version	XM_017320873.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus methylphosphate capping enzyme (Mepce), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320873.1

ORF Insert Sequence:

ATGATCGAGA TGGCGGCGGA GAAGGAGCCC TTTCTGGTGC CGGCCCCGCC GCCGCCGCTC 
AAAGATGAGT CGGGCGGAGG GGGCGGCCCC GAGGTGCAAT CGCACCAAGA GGCTGCCTCC 
GGGGAGCTCC GTGATGGGAC AGAACACGGG CCGGGCCCTC GCGCGCACTC GGCCGGGGCC 
GCAGCCAGCG GAGGGGGCGG CCCCCAGGCA CAGGCGCACG GGGAGCCCCA CGGGCGAGCC 
GCCGCTCCTG CGGACGTGGG GGAGGAGCGC CGGGGAGGGG GCGGGACGGA CCTGGGTCCC 
CCAGCCCCTC CTCGACCTCG CAATGGCTAC CAGCCCCACC GACCCCCTGG GGGTGGTGGG 
GGCAAAAGAA GAAATAGCTG TAACGTGGGG GGAGGGAGCG GTGGAAGCTT CAAACATCCG 
GCCTTCAAGA GGCGCCGGCG AGTGAATTCA GACTGCGACT CTGTGTTACC ATCCAATTTC 
CTTCTGGGGG GTAACATATT TGATCCACTG AACCTGAATA GCCTCCTGGA TGAGGAAGTG 
AGTCGCGCAC TCAATGCGGA GACCCCAAAG TCATCCCCAC TTCCGGCCAA GGGGCGAGAC 
CCTGTGGAGA TCCTCATCCC CAAAGATATT ACTGACCCGC TCAGTCTCAA CACTTGCACT 
GACGAGGCCC ATGTAGTGCT TGCCTCACCA CTCAAGATTG GTCGCAAGCG CCATAGACAC 
CGGGGACCGC ACCACCAGCA GCAGCAGCAG GCGTCTGGAG GGAATGATAG CAACGCCGCC 
GTGCTGCCCA CTGACCCTCT CACGCCCTCA CTCCATGGGG AGGGTGCTAC GCAGCAGCAG 
CAAAACAGAG GCCAGAACCG TGATGCCCCT CAACCCTATG AACTCAACAC AGCCATCAAC 
TGCAGGGATG AAGTTGTGTC TCCCCTTCCA TCTGCCCTGC AGGGTTCCTC AGGCTCCCTT 
TCTGCCCCTC CAGCTGCCTC AGTTACCTCT GCACCCTCTA CTTCTTCCTC CTCCCGACAT 
CGCAAACGTC GCAGGACTTC CAGCAAGTCA GAGGCAGGGG CTAGGGGTGG AAGCCAGGGT 
TCCAAGGAAA AGGGCAGAGG GAGTGGAGGA GGCCGTCACC ACCACCACCC ACTACCTGCA 
ACTGGCTTCA AAAAGCAACA GCTAAAGTTC CAATATGGGA ATTACTGCAA GTACTATGGC 
TATCGCAATC CTTCCTGTGA GGACGTGCGC CTTAGGGTGT TGAAGCCCGA GTGGTTTCAA 
GGCCGGGACG TCCTAGATCT GGGCTGCAAC GTTGGCCATC TGACCCTAAG TATTGCCTGC 
AAGTGGGGCC CAGCCCGCAT GGTGGGGCTG GACATTGATC CACGGCTTAT CCACTCTGCT 
CGCCAAAATA TCCGACACTA CCTGTCGGAG GAGCTTCGTC TCCAAGCTCA GACTTCTGAG 
GGGGACCCAG GGACAGAGGG GGAGGAAGGG ACTATCACTG TCCGAAAAAG GAGCTGCTTC 
CCAGCCTCAC TGACAGCCAG CCGGGGTCCC ATCGCTGCAC CCCAAGTGCC CTTGGATGGA 
GCGGACACAT CTGTCTTCCC CAACAATGTC GTCTTCGTCA CGGGTAACTA TGTCCTGGAC 
CGAGATGAGC TGGTGGATGC CCAAAGACCG GAGTATGATG TGGTGCTGTG CTTCAGCCTC 
ACCAAGTGGG TGCACCTGAA CTGGGGTGAT GAGGGGCTGA AGAGAATGTT CCGCCGCATC 
TACCGGCACC TGCGCCCTGG GGGTATTCTG GTCCTGGAGC CCCAACCATG GTCCTCCTAC 
TGCAAGAGAA AGTCTCTCAC GGAAACAATC TATAAGAACT ACTTTCGAAT CCAGTTGAAG 
CCAGAACAGT TCAGTTCCTA CCTGACATCC CCAGAGGTGG GTTTCTCCAG CTATGAGCTT 
GTGGCCACAC CGAATAACAC CTCCAGAGGT AAGAAGGGCT CGTTTTTTTG GGCTGGGAGG 
GGATCGTCCT GGCTGCAAGG GTATAGGCCC TGTGGAGAAC CCCAGGGTCC TACCCACCTG 
CCTCTGTCTT CCCTTCAGGC TTCCAGCGTC CTGTGTACTT GTTCCACAAG GCTCGATCCC 
CCAGCCACTA AGTGGCCTTC TGAGAAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells.
Genes & development26(22)2477-82(2012 Nov)
Okamura D,Maeda I,Taniguchi H,Tokitake Y,Ikeda M,Ozato K,Mise N,Abe K,Noce T,Izpisua Belmonte JC,Matsui Y

Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome.
Human molecular genetics7(12)1967-78(1998 Nov)
Ko MS,Threat TA,Wang X,Horton JH,Cui Y,Wang X,Pryor E,Paris J,Wells-Smith J,Kitchen JR,Rowe LB,Eppig J,Satoh T,Brant L,Fujiwara H,Yotsumoto S,Nakashima H

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Mepce cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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