Pim3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pim3
Entrez Gene ID	223775
Full Name	proviral integration site 3
Synonyms	BC026639,Kid1
General protein information	Preferred Names proviral integration site 3 Names serine/threonine-protein kinase pim-3 KID-1/kinase induced by depolarization
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_145478.2	NP_663453.1	serine/threonine-protein kinase pim-3

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100497723	XP_002932230.1
Rattus norvegicus (Norway rat)	Pim3	NP_072124.1
Gallus gallus (chicken)	PIM3	XP_003640390.1
	PIM3	XP_001116171.2
Mus musculus (house mouse)	Pim3	NP_663453.1
Homo sapiens (human)	PIM3	NP_001001852.2
Bos taurus (cattle)	PIM3	XP_002688056.3

Gene Ontology
Bibliography

Related articles in PubMed

Metabolic dysfunction consistent with premature aging results from deletion of Pim kinases.
Din S, Konstandin MH, Johnson B, Emathinger J, V?lkers M, Toko H, Collins B, Ormachea L, Samse K, Kubli DA, De La Torre A, Kraft AS, Gustafsson AB, Kelly DP, Sussman MA
Circulation research115(3)376-87(2014 Jul)

Pim1 serine/threonine kinase regulates the number and functions of murine hematopoietic stem cells.
An N, Lin YW, Mahajan S, Kellner JN, Wang Y, Li Z, Kraft AS, Kang Y
Stem cells (Dayton, Ohio)31(6)1202-12(2013 Jun)

Abnormal hematopoietic phenotypes in Pim kinase triple knockout mice.
An N, Kraft AS, Kang Y
Journal of hematology & oncology612(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of PIM3 is associated with abnormal hematopoietic phenotypes.
Title: Abnormal hematopoietic phenotypes in Pim kinase triple knockout mice.

Pim-3 has an important role in modulating c-Myc and PGC-1alpha protein levels and cell growth
Title: The Pim protein kinases regulate energy metabolism and cell growth.

investigation of physiological role of Pim3 in beta-cell function: regulates glucose-stimulated insulin secretion; limits ERK signaling; interacts with SOCS6; Pim3 localized to beta-cell; Pim3 knockout mice display increased glucose tolerance
Title: Pim3 negatively regulates glucose-stimulated insulin secretion.

Pim-3 is expressed in endothelial cells and promotes vascular tube formation.
Title: Pim-3 is expressed in endothelial cells and promotes vascular tube formation.

Pim-3 kinase has a novel role in the control of self-renewal of embryonic stem cells.
Title: Self-renewal of murine embryonic stem cells is supported by the serine/threonine kinases Pim-1 and Pim-3.

The following Pim3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pim3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19628	NM_145478.2 Latest version!	Mus musculus proviral integration site 3 (Pim3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pim3 ( NM_145478.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663453.1 Mus musculus proviral integration site 3 (Pim3), mRNA.

Price & Availability↑

CloneID	OMu19628
Clone ID Related Accession (Same CDS sequence)	NM_145478.2
Accession Version	NM_145478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase pim-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC117700.22. On Nov 17, 2006 this sequence version replaced NM_145478.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017621.1, AK168014.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCTGT CCAAGTTCGG CTCCCTGGCG CACCTCTGCG GGCCTGGCGG CGTGGACCAC 
CTCCCAGTGA AGATCCTACA GCCAGCCAAG GCTGACAAGG AGAGCTTCGA GAAGGTGTAC 
CAGGTGGGCG CCGTGCTGGG CAGCGGCGGC TTCGGCACGG TCTACGCGGG CAGCCGCATC 
GCCGACGGAC TCCCGGTGGC TGTGAAGCAC GTGGTGAAGG AGCGGGTGAC CGAGTGGGGC 
AGTCTCGGCG GAGTGGCCGT GCCCCTGGAG GTGGTGCTGC TGCGCAAGGT GGGCGCGGCG 
GGCGGCGCGC GCGGCGTCAT CCGCTTGCTG GACTGGTTCG AGCGGCCCGA CGGCTTCTTG 
TTGGTGCTGG AGCGACCCGA GCCGGCACAG GACCTCTTCG ACTTCATCAC TGAACGAGGC 
GCCCTGGACG AGCCGCTGGC GCGTCGCTTC TTCGCGCAGG TGCTTGCCGC TGTGCGGCAC 
TGCCACAATT GTGGGGTCGT GCACCGCGAC ATCAAGGACG AGAACCTGCT GGTGGACCTG 
CGCTCGGGAG AGCTGAAGCT CATCGACTTC GGCTCGGGCG CGGTGCTCAA GGACACGGTC 
TACACTGACT TTGATGGCAC CCGTGTGTAC AGCCCCCCAG AGTGGATCCG ATATCACCGA 
TATCACGGGC GGTCTGCCAC TGTGTGGTCT CTGGGTGTAC TGCTCTACGA CATGGTGTGT 
GGGGACATTC CCTTTGAGCA GGATGAGGAG ATCTTGCGCG GCAGGCTCTT TTTCCGGAGG 
AGGGTCTCCC CAGAGTGCCA GCAGCTTATT GAGTGGTGTC TCTCCCTGAG GCCCTCAGAG 
AGGCCCTCCC TGGACCAAAT TGCTGCCCAC CCCTGGATGC TGGGGACAGA GGGGAGCGTT 
CCAGAGAACT GTGACCTTCG GCTTTGTGCC CTGGATACTG ACGACGGAGC CAGTACCACT 
TCCAGCAGTG AGAGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663453.1
CDS	475..1455
Translation	MLLSKFGSLAHLCGPGGVDHLPVKILQPAKADKESFEKVYQVGAVLGSGGFGTVYAGSRIADGLPVAVKHVVKERVTEWGSLGGVAVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGFLLVLERPEPAQDLFDFITERGALDEPLARRFFAQVLAAVRHCHNCGVVHRDIKDENLLVDLRSGELKLIDFGSGAVLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLFFRRRVSPECQQLIEWCLSLRPSERPSLDQIAAHPWMLGTEGSVPENCDLRLCALDTDDGASTTSSSESL

Target ORF information:

RefSeq Version	NM_145478.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus proviral integration site 3 (Pim3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145478.2

ORF Insert Sequence:

ATGCTGCTGT CCAAGTTCGG CTCCCTGGCG CACCTCTGCG GGCCTGGCGG CGTGGACCAC 
CTCCCAGTGA AGATCCTACA GCCAGCCAAG GCTGACAAGG AGAGCTTCGA GAAGGTGTAC 
CAGGTGGGCG CCGTGCTGGG CAGCGGCGGC TTCGGCACGG TCTACGCGGG CAGCCGCATC 
GCCGACGGAC TCCCGGTGGC TGTGAAGCAC GTGGTGAAGG AGCGGGTGAC CGAGTGGGGC 
AGTCTCGGCG GAGTGGCCGT GCCCCTGGAG GTGGTGCTGC TGCGCAAGGT GGGCGCGGCG 
GGCGGCGCGC GCGGCGTCAT CCGCTTGCTG GACTGGTTCG AGCGGCCCGA CGGCTTCTTG 
TTGGTGCTGG AGCGACCCGA GCCGGCACAG GACCTCTTCG ACTTCATCAC TGAACGAGGC 
GCCCTGGACG AGCCGCTGGC GCGTCGCTTC TTCGCGCAGG TGCTTGCCGC TGTGCGGCAC 
TGCCACAATT GTGGGGTCGT GCACCGCGAC ATCAAGGACG AGAACCTGCT GGTGGACCTG 
CGCTCGGGAG AGCTGAAGCT CATCGACTTC GGCTCGGGCG CGGTGCTCAA GGACACGGTC 
TACACTGACT TTGATGGCAC CCGTGTGTAC AGCCCCCCAG AGTGGATCCG ATATCACCGA 
TATCACGGGC GGTCTGCCAC TGTGTGGTCT CTGGGTGTAC TGCTCTACGA CATGGTGTGT 
GGGGACATTC CCTTTGAGCA GGATGAGGAG ATCTTGCGCG GCAGGCTCTT TTTCCGGAGG 
AGGGTCTCCC CAGAGTGCCA GCAGCTTATT GAGTGGTGTC TCTCCCTGAG GCCCTCAGAG 
AGGCCCTCCC TGGACCAAAT TGCTGCCCAC CCCTGGATGC TGGGGACAGA GGGGAGCGTT 
CCAGAGAACT GTGACCTTCG GCTTTGTGCC CTGGATACTG ACGACGGAGC CAGTACCACT 
TCCAGCAGTG AGAGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Metabolic dysfunction consistent with premature aging results from deletion of Pim kinases.
Circulation research115(3)376-87(2014 Jul)
Din S,Konstandin MH,Johnson B,Emathinger J,V?lkers M,Toko H,Collins B,Ormachea L,Samse K,Kubli DA,De La Torre A,Kraft AS,Gustafsson AB,Kelly DP,Sussman MA

Pim1 serine/threonine kinase regulates the number and functions of murine hematopoietic stem cells.
Stem cells (Dayton, Ohio)31(6)1202-12(2013 Jun)
An N,Lin YW,Mahajan S,Kellner JN,Wang Y,Li Z,Kraft AS,Kang Y

Abnormal hematopoietic phenotypes in Pim kinase triple knockout mice.
Journal of hematology & oncology612(2013 Jan)
An N,Kraft AS,Kang Y